Journal of Biophotonics最新文献

{"title":"fNIRS-Based Network Signatures of Sensory-Primed Bilateral Arm Training in Subacute Stroke Recovery","authors":"Wenhao Li,&nbsp;Bing Wang,&nbsp;Qiang Zhang,&nbsp;Ran Wei,&nbsp;Congcong Huo","doi":"10.1002/jbio.70353","DOIUrl":"https://doi.org/10.1002/jbio.70353","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Significance</h3>\u0000 \u0000 <p>This study leverages functional near-infrared spectroscopy (fNIRS) to elucidate optically derived cortical network signatures underlying combined sensory–motor interventions in stroke rehabilitation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To characterize the synergistic effects of peripheral electrical stimulation (PES) and bilateral arm training (BAT) on cortical hemodynamics and functional network architecture using fNIRS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Approach</h3>\u0000 \u0000 <p>fNIRS signals were acquired from 24 subacute stroke patients. Wavelet amplitude and wavelet phase coherence were employed to quantify task-related hemodynamic activation and interregional synchronization within the 0.01–0.08 Hz frequency band.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Compared with BAT alone, the PES-BAT paradigm elicited augmented hemodynamic activation in ipsilesional prefrontal and motor regions. The combined intervention further enhanced phase synchronization across distributed cortical networks and preserved interhemispheric coherence, counteracting maladaptive contralesional dominance.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The fNIRS-based wavelet metrics effectively detect intervention-dependent modulation of cortical hemodynamics and network organization, highlighting the value of optical neuroimaging in assessing neuroplasticity during stroke rehabilitation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autofluorescence Imaging and Methylene Blue-Mediated Photodynamic Therapy for Early Detection and Management of Subclinical Actinic Cheilitis","authors":"Juliana Cristina Oliverio de Araújo,&nbsp;Og Fray Junior,&nbsp;Hermano Camelo Paiva,&nbsp;Alyne Simões Gonçalves,&nbsp;Martha Simões Ribeiro","doi":"10.1002/jbio.70346","DOIUrl":"https://doi.org/10.1002/jbio.70346","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Optical autofluorescence imaging is a noninvasive adjunct for detecting potentially malignant oral disorders, such as actinic cheilitis (AC). This study evaluated its use for early detection and for monitoring tissue response following methylene blue-mediated photodynamic therapy (MB-PDT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Lip autofluorescence was assessed using a handheld device (405 ± 20 nm). Areas with altered fluorescence underwent biopsy for confirmation. Patients with AC received five MB-PDT sessions (660 nm, 100 mW, 120 s/point) after topical 1% MB. Grayscale intensity was quantified before treatment and at 30 days.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of 25 screened individuals, 19 had confirmed AC, and 17 completed follow-up. Lesions showed higher fluorescence intensity than healthy tissue. After MB-PDT, mean intensity and variability significantly decreased, indicating tissue response. Smokers showed a smaller reduction.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Autofluorescence imaging enables early detection and noninvasive monitoring of AC. Combined with MB-PDT, it represents a practical strategy for managing high-risk patients and supporting early intervention.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70346","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Does the Photoplethysmography Add to Laser Doppler Flowmetry During the Brachial Artery Occlusion Test?","authors":"Vasily Karpov,&nbsp;Alexey Glazkov,&nbsp;Denis Lapitan,&nbsp;Aleksandra Ivlieva,&nbsp;Dariya Selivanova,&nbsp;Dmitry Rogatkin","doi":"10.1002/jbio.70347","DOIUrl":"https://doi.org/10.1002/jbio.70347","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, the difference in behavior of the raw photoplethysmography (PPG) signal and the laser Doppler flowmetry (LDF) signal reflecting hemodynamics in the skin of upper extremities at brachial arterial occlusion is recorded and discussed. It is shown that at various locations on the hand, the raw PPG signal provides richer and previously little-known information on the redistribution of blood between the arterial, microcirculatory, and venous beds, while the conventional blood flow (BF) parameter recorded by LDF technique is less sensitive to it and largely does not capture this redistribution.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vivo Monitoring of Pressure Ulcer Model Development and Recoverability Under Multimodal Parameters Within a Unified Field of View","authors":"Qimeng Liu,&nbsp;Yixin Wang,&nbsp;Mu Chen,&nbsp;Shihao Tang,&nbsp;Yamin Yang,&nbsp;Xiaofei Jin,&nbsp;Jie Dai,&nbsp;Yameng Zhang,&nbsp;Weitao Li","doi":"10.1002/jbio.70348","DOIUrl":"https://doi.org/10.1002/jbio.70348","url":null,"abstract":"<div>\u0000 \u0000 <p>This study utilizes an integrated laser speckle (LSCI) and intrinsic optical signal imaging (IOSI) system to evaluate microvascular dynamics in murine pressure ulcer (PU) models. We compared a single prolonged compression (6 h) against repeated compressions (1.5 h/day for 7 days) using an enhanced spatiotemporal gradient analysis of LSCI data. In the repeated compression model, gradient analysis revealed a progressive perfusion decline by Day 4. By Day 7, perfusion gradients indicated near-complete flow loss, corresponding to severe hypoxia detected by IOSI, signifying sustained microvascular impairment. Conversely, the single compression model exhibited reversible alterations; LSCI gradients showed initial disruption followed by gradual recovery, with perfusion nearing baseline by Day 7. The LSCI-IOSI platform, emphasizing advanced gradient processing, systematically delineates microcirculatory compromise. This approach highlights the critical role of repeated ischemia–reperfusion in driving persistent dysfunction and demonstrates the utility of quantitative optical imaging for early detection and targeted intervention in PU progression.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep Learning-Based Inclusion Boundary Identification Using Wave Propagation in Optical Coherence Elastography","authors":"Guangyu Zhang,&nbsp;Jinpeng Liao,&nbsp;Zhengshuyi Feng,&nbsp;Alison M. Layton,&nbsp;Chunhui Li,&nbsp;Zhihong Huang","doi":"10.1002/jbio.70345","DOIUrl":"10.1002/jbio.70345","url":null,"abstract":"<p>Elastography is widely used to estimate tissue mechanical properties based on wave propagation characteristics. However, a major limitation is that elastography maps often fail to accurately delineate internal boundaries within heterogeneous tissues. To address this issue, we propose a novel deep learning–based framework, termed Boundary Detection Network (BDNet). Depth-resolved phase images capturing wave propagation are acquired using Optical Coherence Elastography (OCE). The proposed method is first evaluated on agar phantoms. Experimental results show that all evaluated models achieve comparable performance on single-boundary samples, with a mean absolute error (MAE) of 2 pixels. For dual-boundary phantoms, BDNet achieves an overall MAE of 4.33 pixels. Furthermore, on a human acne dataset, the proposed method provides the best performance, with an MAE of 19.11 pixels. The proposed BDNet demonstrates high accuracy and computational efficiency, providing a promising tool for assisting abnormal tissue morphology identification in clinical elastography applications.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13500869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148809727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Assessment of Composition-Dependent Mechanical Properties of Atherosclerotic Plaques by Photoacoustic Elasticity and Viscosity Imaging","authors":"Ao Jiang,&nbsp;Yuxuan Liang,&nbsp;Yiting Tan,&nbsp;Heng Zhang,&nbsp;Pingping Wang","doi":"10.1002/jbio.70342","DOIUrl":"https://doi.org/10.1002/jbio.70342","url":null,"abstract":"<div>\u0000 \u0000 <p>Evaluating the mechanical properties of atherosclerotic plaques is crucial for precisely identifying rupture-prone lesions, as different plaque constituents dictate local biomechanical instability and directly inform targeted therapeutic intervention. In this study, we further achieved quantitative photoacoustic elasticity imaging (PAEI) and photoacoustic viscosity imaging (PAVI) by analyzing the rise time of the thermoelastic displacement and by measuring the phase delay of the photoacoustic signal relative to the laser pulse, respectively. Experiments on mice aortas have shown that PAVI is more sensitive to the changes in lipid core area during lipid plaque stage, whereas PAEI is more effective at detecting changes in fibrous cap thickness during fibrous plaque stage. Furthermore, PAEI and PAVI can effectively distinguish lipid plaques and fibrous plaques from normal vascular tissue. All results indicate that the PAEI–PAVI imaging system enables label-free, high-resolution visualization of the component-based biomechanical properties of atherosclerotic plaques, facilitating early detection and rupture risk assessment.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intensity Variance-Guided Automated Robotic Optical Coherence Tomography System for Ophthalmic Applications","authors":"Hang Su,&nbsp;Jie Zhang,&nbsp;Maoyuan Qu,&nbsp;Xiru Gao,&nbsp;Hongqin Chen,&nbsp;Congyu Hu,&nbsp;Pengfei Song,&nbsp;Xingchen Ji,&nbsp;Yikai Su","doi":"10.1002/jbio.70343","DOIUrl":"https://doi.org/10.1002/jbio.70343","url":null,"abstract":"<div>\u0000 \u0000 <p>Optical coherence tomography (OCT) enables non-invasive volumetric retinal imaging. Conventional tabletop and handheld systems rely on skilled operators and patient cooperation. Mobile robot-assisted OCT (RAOCT) systems can address these challenges, but many depend on complex visual servo modules for low-latency eye tracking. We present an intensity variance-guided RAOCT system integrated on a wheeled mobile platform. The system uses one depth camera for coarse eye localization and a single pupil camera for real-time tracking. By calibrating a lookup table between image variance and distance within the near-eye region, the pupil camera provides indirect depth estimation for robotic servoing. A motorized reference arm, an electrically tunable lens, and an automated polarization controller further enable image-quality optimization. Experiments demonstrated 103.50 μm axial and 20.46 μm lateral tracking accuracy. The pupil-camera response time was 10.53 ms. Automated retinal OCT imaging was achieved, demonstrating the system's potential for point-of-care diagnostics in resource-limited environments.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multispectral Photoacoustic Imaging Detects Altered Hepatic Hemodynamics and Lipid in Early STZ-Induced Type 1 Diabetic Liver Injury","authors":"Tingting Qiu,&nbsp;Jiazhi Cao,&nbsp;Xijing Yang,&nbsp;Yan Luo,&nbsp;Hong Wang,&nbsp;Wenwu Ling","doi":"10.1002/jbio.70344","DOIUrl":"https://doi.org/10.1002/jbio.70344","url":null,"abstract":"<div>\u0000 \u0000 <p>Diabetic liver injury (DLI) is a major complication of diabetes mellitus (DM), disrupting hepatic glycogen and lipid metabolism. This study adopted photoacoustic imaging (PAI) to detect hepatic blood oxygen saturation, hemoglobin content, lipid deposition, and liver function reserve (LFR) in type 1 diabetic rodent models. Compared with the control group, the T1DM group presented significantly higher average hepatic oxygen saturation (<i>p</i> = 0.014), lower total hemoglobin (<i>p</i> &lt; 0.001), and decreased lipid photoacoustic signal intensity (<i>p</i> = 0.045). No obvious difference was found in LFR between the two groups. In summary, PAI holds promising potential for reflection of altered hepatic hemodynamics and lipid in early STZ-induced type 1 diabetic liver injury.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vivo Monitoring of Facial Acne Development by Using Multimodal Functional OCT","authors":"Zhengshuyi Feng,&nbsp;Jinpeng Liao,&nbsp;Tianyu Zhang,&nbsp;Guangyu Zhang,&nbsp;Alison M. Layton,&nbsp;Tarl Prow,&nbsp;Chunhui Li,&nbsp;Zhihong Huang","doi":"10.1002/jbio.70337","DOIUrl":"https://doi.org/10.1002/jbio.70337","url":null,"abstract":"<div>\u0000 \u0000 <p>Acne is a common inflammatory skin condition and frequently leads to long-term adverse clinical outcomes including scarring and pigmentation, negatively impacting Health Related Quality of Life (HRQoL). Monitoring of acne progression can improve understanding of lesion evolution, support evaluation of treatment response and provide potential biomarkers for future investigation of acne sequelae. This study presents a multi-functional optical coherence tomography (OCT) protocol integrating structural imaging, OCT-based angiography (OCTA), and optical coherence elastography (OCE) for longitudinal in vivo monitoring of acne. Thirteen lesions were evaluated across eight time points over 18 days. Quantitative parameters, including Young's modulus and vascular metrics, captured biomechanical and microvascular changes during acne evolution. The system enables continuous multimodal assessment, showing that stiffness and vascular alterations reflect lesion progression. This pilot longitudinal study establishes a quantitative foundation for dermatological research and suggests that multimodal OCT monitoring may provide biomarkers for assessing lesion progression and long-term clinical outcomes.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linear Variable Filter-Based Wide-Field Hyperspectral Camera for Multiplex Immunoplasmonics Imaging","authors":"Cécile Darviot,&nbsp;Michel Meunier","doi":"10.1002/jbio.70341","DOIUrl":"10.1002/jbio.70341","url":null,"abstract":"<p>Hyperspectral imaging combined with plasmonic nanoparticles offers powerful opportunities for nanoscale biological diagnostics, yet conventional push-broom systems are limited by slow acquisition and reduced spatial resolution. We report a wide-field hyperspectral imaging platform integrating a custom linear variable tunable filter (LVTF) with reflected light microscopy (RLM) for multiplexed plasmonic nanoparticle (NP) imaging. While LVTF-based systems have been used in industrial contexts, their adaptation to RLM microscopy enables high-resolution, high-contrast spectral imaging of NPs on biological samples. The system acquires data across 380–720 nm with 7–20 nm spectral resolution while preserving spatial detail. We demonstrate imaging of NPs bound to cancer cell membranes and classification of four distinct NP types. A trained support vector machine achieves an overall <i>F</i>-score of ~97%, significantly outperforming RGB-based approaches. Compared to push-broom systems, this platform provides faster acquisition, improved spatial resolution, and a compact, cost-effective solution for multiplex NP detection in bioimaging and immunoplasmonic applications.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70341","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148714929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}