Journal of Biophotonics最新文献_第10页

5-ALA mediated photodynamic therapy increases natural killer cytotoxicity against oral squamous cell carcinoma cell lines 5-ALA 介导的光动力疗法可提高自然杀伤细胞对口腔鳞状细胞癌细胞株的细胞毒性。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-07-18 DOI: 10.1002/jbio.202400176

Angela Cristina Molon, Daniele Heguedusch, Fabio Daumas Nunes, Rebeca Boltes Cecatto, Adriana Lino dos Santos Franco, Camila de Oliveira Rodini Pegoraro, Maria Fernanda Setúbal Destro Rodrigues

{"title":"5-ALA mediated photodynamic therapy increases natural killer cytotoxicity against oral squamous cell carcinoma cell lines","authors":"Angela Cristina Molon, Daniele Heguedusch, Fabio Daumas Nunes, Rebeca Boltes Cecatto, Adriana Lino dos Santos Franco, Camila de Oliveira Rodini Pegoraro, Maria Fernanda Setúbal Destro Rodrigues","doi":"10.1002/jbio.202400176","DOIUrl":"10.1002/jbio.202400176","url":null,"abstract":"Oral squamous cell carcinoma (OSCC) constitutes over 90% of oral cancers, known for its aggressiveness and poor prognosis. Photodynamic therapy (PDT) has emerged as a promising adjuvant therapy and is linked to immunogenic cell death, activating innate and adaptive anti-tumor responses. Natural Killer (NK) cells, key players in malignant cell elimination, have not been extensively studied in PDT. This study evaluates whether PDT increases OSCC cell lines' susceptibility to NK cell cytotoxicity. PDT, using 5-aminolevulinic acid (5-ALA) and LED irradiation, was applied to Ca1 and Luc4 cell lines. Results showed a dose-dependent viability decrease post-PDT. Gene expression analysis revealed upregulation of NK cell-activating ligands (ULBP1-4, MICA/B) and decreased MHC class I expression in Ca1, suggesting increased NK cell susceptibility. Enhanced NK cell cytotoxicity was confirmed in Ca1 but not in Luc4 cells. These findings indicate that PDT may enhance NK cell-mediated cytotoxicity in OSCC, offering potential for improved treatment strategies.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The synergistic effect of photodynamic and sonodynamic inactivation against Candida albicans biofilm 光动力和声动力灭活对白色念珠菌生物膜的协同效应。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-07-17 DOI: 10.1002/jbio.202400190

Gabriela Gomes Guimarães, Fernanda Alves, Isabella Gonçalves, Iago Silva e Carvalho, Erika Toneth Ponce Ayala, Sebastião Pratavieira, Vanderlei Salvador Bagnato

{"title":"The synergistic effect of photodynamic and sonodynamic inactivation against Candida albicans biofilm","authors":"Gabriela Gomes Guimarães, Fernanda Alves, Isabella Gonçalves, Iago Silva e Carvalho, Erika Toneth Ponce Ayala, Sebastião Pratavieira, Vanderlei Salvador Bagnato","doi":"10.1002/jbio.202400190","DOIUrl":"10.1002/jbio.202400190","url":null,"abstract":"Candida albicans biofilm can cause diseases that are resistant to conventional antifungal agents. Photodynamic (PDI), sonodynamic (SDI), and sonophotodynamic (SPDI) inactivation have arisen as promising antimicrobial strategies. This study evaluated these treatments mediated by curcumin against C. albicans biofilms. For this, C. albicans biofilms were submitted to PDI, SDI, or SPDI with different light and ultrasound doses, then, the viability assay was performed to measure the effectiveness. Finally, a mathematical model was suggested to fit acquired experimental data and understand the synergistic effect of light and ultrasound in different conditions. The results showed that SPDI, PDI, and SDI reduced the viability in 6 ± 1; 1 ± 1; and 2 ± 1 log, respectively, using light at 60 J/cm2, ultrasound at 3 W/cm2, and 80 μM of curcumin. The viability reduction was proportional to the ultrasound and light doses delivered. These results encourage the use of SPDI for the control of microbial biofilm.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A practical solution to improve the field of view in circular scanning-based photoacoustic tomography 改善基于环形扫描的光声断层扫描视野的实用解决方案。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-07-12 DOI: 10.1002/jbio.202400125

Seyed Mohsen Ranjbaran, Mohsin Zafar, Loïc Saint-Martin, Md Tarikuls Islam, Kamran Avanaki

引用次数: 0

Ultrabroadband two-beam coherent anti-Stokes Raman scattering and spontaneous Raman spectroscopy of organic fluids: A comparative study 有机流体的超宽带双光束相干反斯托克斯拉曼散射和自发拉曼光谱：比较研究。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-07-09 DOI: 10.1002/jbio.202300505

Timea Koch, Roland Ackermann, Axel Stoecker, Tobias Meyer-Zedler, Thomas Gabler, Tom Lippoldt, Jeannine Missbach-Guentner, Christoph Russmann, Jürgen Popp, Stefan Nolte

{"title":"Ultrabroadband two-beam coherent anti-Stokes Raman scattering and spontaneous Raman spectroscopy of organic fluids: A comparative study","authors":"Timea Koch, Roland Ackermann, Axel Stoecker, Tobias Meyer-Zedler, Thomas Gabler, Tom Lippoldt, Jeannine Missbach-Guentner, Christoph Russmann, Jürgen Popp, Stefan Nolte","doi":"10.1002/jbio.202300505","DOIUrl":"10.1002/jbio.202300505","url":null,"abstract":"Spontaneous Raman spectroscopy is a well-established diagnostic tool, allowing for the identification of all Raman active species with a single measurement. Yet, it may suffer from low-signal intensity and fluorescent background. In contrast, coherent anti-Stokes Raman scattering (CARS) offers laser-like signals, but the traditional approach lacks the multiplex capability of spontaneous Raman spectroscopy. We present an ultrabroadband CARS setup which aims at exciting the full spectrum (300–3700 cm−1) of biological molecules. A dual-output optical parametric amplifier provides a ~7 fs pump/Stokes and a ~700 fs probe pulse. CARS spectra of DMSO, ethanol, and methanol show great agreement with spontaneous Raman spectroscopy and superiority in fluorescent environments. The spectral resolution proves sufficient to differentiate between the complex spectra of L-proline and hydroxyproline. Moreover, decay constants in the sub picosecond range are determined for individual Raman transitions, providing an additional approach for sample characterization.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202300505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between amide ratio assessed by Fourier-transform infrared spectroscopy: A biomarker candidate for polycythemia vera disease 傅立叶变换红外光谱法评估的酰胺比率之间的关系：多发性红细胞症的候选生物标志物

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-07-08 DOI: 10.1002/jbio.202400162

Zozan Guleken, Aynur Aday, Ayşe Gül Bayrak, İpek Yönal Hindilerden, Meliha Nalçacı, Jozef Cebulski, Joanna Depciuch

引用次数: 0

Toward noncontact macroscopic imaging of multiple cancers using multi-spectral inelastic scattering detection 利用多光谱非弹性散射检测技术实现多发性癌症的非接触式宏观成像。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-07-04 DOI: 10.1002/jbio.202400087

Sandryne David, Nassim Ksantini, Frédérick Dallaire, Katherine Ember, François Daoust, Guillaume Sheehy, Costas G. Hadjipanayis, Kevin Petrecca, Brian C. Wilson, Frédéric Leblond

{"title":"Toward noncontact macroscopic imaging of multiple cancers using multi-spectral inelastic scattering detection","authors":"Sandryne David, Nassim Ksantini, Frédérick Dallaire, Katherine Ember, François Daoust, Guillaume Sheehy, Costas G. Hadjipanayis, Kevin Petrecca, Brian C. Wilson, Frédéric Leblond","doi":"10.1002/jbio.202400087","DOIUrl":"10.1002/jbio.202400087","url":null,"abstract":"Here we introduce a Raman spectroscopy approach combining multi-spectral imaging and a new fluorescence background subtraction technique to image individual Raman peaks in less than 5 seconds over a square field-of-view of 1-centimeter sides with 350 micrometers resolution. First, human data is presented supporting the feasibility of achieving cancer detection with high sensitivity and specificity – in brain, breast, lung, and ovarian/endometrium tissue – using no more than three biochemically interpretable biomarkers associated with the inelastic scattering signal from specific Raman peaks. Second, a proof-of-principle study in biological tissue is presented demonstrating the feasibility of detecting a single Raman band – here the CH2/CH3 deformation bands from proteins and lipids – using a conventional multi-spectral imaging system in combination with the new background removal method. This study paves the way for the development of a new Raman imaging technique that is rapid, label-free, and wide field.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of low-dose photodynamic therapy using the photosensitizer chloroaluminum phthalocyanine on a scratch wound model in skin fibroblasts 使用光敏剂氯铝酞菁的低剂量光动力疗法对皮肤成纤维细胞划痕伤口模型的影响。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-07-04 DOI: 10.1002/jbio.202400033

Efstathios Giannakopoulos, Annita Katopodi, Michail Rallis, Konstantinos Politopoulos, Eleni Alexandratou

{"title":"The effect of low-dose photodynamic therapy using the photosensitizer chloroaluminum phthalocyanine on a scratch wound model in skin fibroblasts","authors":"Efstathios Giannakopoulos, Annita Katopodi, Michail Rallis, Konstantinos Politopoulos, Eleni Alexandratou","doi":"10.1002/jbio.202400033","DOIUrl":"10.1002/jbio.202400033","url":null,"abstract":"Different approaches on wound healing have been developed over the years but they suffer from high costs and adverse effects for the patients. The current paper was designed to study low dose PDT, a novel healing approach, in an in vitro fibroblasts wound healing model. Chloroaluminum phthalocyanine (AlClPc) was used as photosensitizer and was activated by a red diode laser at 661 nm. After PDT optimization, wound closure rate and reactive oxygen species were quantified by image processing and analysis. Our results revealed that wound healing rates were significantly higher in PDT treated groups than in the control. Additionally, the study revealed that a prolonged ROS increase did not promote wound closure, while a small increase acted as a trigger, resulting in faster wound closure. Concluding, low dose PDT using AlClPc enhances wound healing in vitro in a ROS dependent manner, allowing the assumption of similar positive effects in vivo.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unpaired fundus image enhancement based on constrained generative adversarial networks 基于约束生成式对抗网络的非配对眼底图像增强。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-07-04 DOI: 10.1002/jbio.202400168

Luyao Yang, Shenglan Yao, Pengyu Chen, Mei Shen, Suzhong Fu, Jiwei Xing, Yuxin Xue, Xin Chen, Xiaofei Wen, Yang Zhao, Wei Li, Heng Ma, Shiying Li, Valery V. Tuchin, Qingliang Zhao

{"title":"Unpaired fundus image enhancement based on constrained generative adversarial networks","authors":"Luyao Yang, Shenglan Yao, Pengyu Chen, Mei Shen, Suzhong Fu, Jiwei Xing, Yuxin Xue, Xin Chen, Xiaofei Wen, Yang Zhao, Wei Li, Heng Ma, Shiying Li, Valery V. Tuchin, Qingliang Zhao","doi":"10.1002/jbio.202400168","DOIUrl":"10.1002/jbio.202400168","url":null,"abstract":"Fundus photography (FP) is a crucial technique for diagnosing the progression of ocular and systemic diseases in clinical studies, with wide applications in early clinical screening and diagnosis. However, due to the nonuniform illumination and imbalanced intensity caused by various reasons, the quality of fundus images is often severely weakened, brings challenges for automated screening, analysis, and diagnosis of diseases. To resolve this problem, we developed strongly constrained generative adversarial networks (SCGAN). The results demonstrate that the quality of various datasets were more significantly enhanced based on SCGAN, simultaneously more effectively retaining tissue and vascular information under various experimental conditions. Furthermore, the clinical effectiveness and robustness of this model were validated by showing its improved ability in vascular segmentation as well as disease diagnosis. Our study provides a new comprehensive approach for FP and also possesses the potential capacity to advance artificial intelligence-assisted ophthalmic examination.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400168","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subpixel motion artifacts correction and motion estimation for 3D-OCT 用于 3D-OCT 的亚像素运动伪影校正和运动估算。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-07-02 DOI: 10.1002/jbio.202400104

Xiao Zhang, Haozhe Zhong, Sainan Wang, Bin He, Liangqi Cao, Ming Li, Miaowen Jiang, Qin Li

{"title":"Subpixel motion artifacts correction and motion estimation for 3D-OCT","authors":"Xiao Zhang, Haozhe Zhong, Sainan Wang, Bin He, Liangqi Cao, Ming Li, Miaowen Jiang, Qin Li","doi":"10.1002/jbio.202400104","DOIUrl":"10.1002/jbio.202400104","url":null,"abstract":"A number of hardware-based and software-based strategies have been suggested to eliminate motion artifacts for improvement of 3D-optical coherence tomography (OCT) image quality. However, the hardware-based strategies have to employ additional hardware to record motion compensation information. Many software-based strategies have to need additional scanning for motion correction at the expense of longer acquisition time. To address this issue, we propose a motion artifacts correction and motion estimation method for OCT volumetric imaging of anterior segment, without requirements of additional hardware and redundant scanning. The motion correction effect with subpixel accuracy for in vivo 3D-OCT has been demonstrated in experiments. Moreover, the physiological information of imaging object, including respiratory curve and respiratory rate, has been experimentally extracted using the proposed method. The proposed method offers a powerful tool for scientific research and clinical diagnosis in ophthalmology and may be further extended for other biomedical volumetric imaging applications.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stitching method for panoramic nail fold images based on capillary contour enhancement 基于毛细血管轮廓增强的指甲褶皱全景图像拼接方法。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-07-02 DOI: 10.1002/jbio.202400105

Jianan Lin, Hao Yin, Yanxiong Wu, Jiaxiong Luo, Qianyao Ye, Bin Zhou, Mugui Xie, Cong Ye, Junzhao Liang, Xiaosong Li, Wei Bin, Zhimin Yang

{"title":"Stitching method for panoramic nail fold images based on capillary contour enhancement","authors":"Jianan Lin, Hao Yin, Yanxiong Wu, Jiaxiong Luo, Qianyao Ye, Bin Zhou, Mugui Xie, Cong Ye, Junzhao Liang, Xiaosong Li, Wei Bin, Zhimin Yang","doi":"10.1002/jbio.202400105","DOIUrl":"10.1002/jbio.202400105","url":null,"abstract":"Nail fold capillaroscopy is an important means of monitoring human health. Panoramic nail fold images improve the efficiency and accuracy of examinations. However, the acquisition of panoramic nail fold images is seldom studied and the problem manifests of few matching feature points when image stitching is used for such images. Therefore, this paper presents a method for panoramic nail fold image stitching based on vascular contour enhancement, which first solves the problem of few matching feature points by pre-processing the image with contrast-constrained adaptive histogram equalization (CLAHE), bilateral filtering (BF), and sharpening algorithms. The panoramic images of the nail fold blood vessels are then successfully stitched using the fast robust feature (SURF), fast library of approximate nearest neighbors (FLANN) and random sample agreement (RANSAC) algorithms. The experimental results show that the panoramic image stitched by this paper's algorithm has a field of view width of 7.43 mm, which improves the efficiency and accuracy of diagnosis.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0