{"title":"An overview of current assessment techniques for evaluating cutaneous perfusion in reconstructive surgery","authors":"Lukas Sebastian Fiedler, Houda Daaloul","doi":"10.1002/jbio.202400002","DOIUrl":"10.1002/jbio.202400002","url":null,"abstract":"<p>This article provides a comprehensive analysis of modern techniques used in the assessment of cutaneous flaps in reconstructive surgery. It emphasizes the importance of preoperative planning and intra- and perioperative assessment of flap perfusion to ensure successful outcomes. Despite technological advancements, direct clinical assessment remains the gold standard. We categorized assessment techniques into non-invasive and invasive modalities, discussing their strengths and weaknesses. Non-invasive methods, such as acoustic Doppler sonography, near-infrared spectroscopy, hyperspectral imaging thermal imaging, and remote-photoplethysmography, offer accessibility and safety but may sacrifice specificity. Invasive techniques, including contrast-enhanced ultrasound, computed tomography angiography, near-infrared fluorescence angiography with indocyanine green, and implantable Doppler probe, provide high accuracy but introduce additional risks. We emphasize the need for a tailored decision-making process based on specific clinical scenarios, patient characteristics, procedural requirements, and surgeon expertise. It also discusses potential future advancements in flap assessment, including the integration of artificial intelligence and emerging technologies.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140570386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wilmer Carrión-Roca, Annette M. Colón-Mercado, John R. Castro-Suarez, Edwin R. Caballero-Agosto, Francheska M. Colón-González, José A. Centeno-Ortiz, Carlos Ríos-Velázquez, Samuel P. Hernández-Rivera
{"title":"Chemical sensing of common microorganisms found in biopharmaceutical industries using MIR laser spectroscopy and multivariate analysis","authors":"Wilmer Carrión-Roca, Annette M. Colón-Mercado, John R. Castro-Suarez, Edwin R. Caballero-Agosto, Francheska M. Colón-González, José A. Centeno-Ortiz, Carlos Ríos-Velázquez, Samuel P. Hernández-Rivera","doi":"10.1002/jbio.202300391","DOIUrl":"10.1002/jbio.202300391","url":null,"abstract":"<p>Mid-infrared laser spectroscopy was used to investigate common bacteria encountered in biopharmaceutical industries. The study involved the detection of bacteria using quantum cascade laser spectroscopy coupled to a grazing angle probe (QCL-GAP). Substrates similar to surfaces commonly used in biopharmaceutical industries were used as support media for the samples. Reflectance measurements were assisted by Multivariate Analysis (MVA) to assemble a powerful spectroscopic technique with classification and identification resources. The species analyzed, <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, and <i>Micrococcus luteus</i>, were used to challenge the technique's capability to discriminate from microorganisms of the same family. Principal Components Analysis and Partial Least Squares-Discriminant Analysis differentiated between the bacterial species, using QCL-GAP-MVA as the reference. Spectral differences in the bacterial membrane were used to determine if these microorganisms were present in the samples analyzed. Results herein provided effective discrimination for the bacteria under study with high sensitivity and specificity.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140570393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Angze Li, Xiaoling Wei, Yajia Xie, Yi Ren, Xi Zhu, Muqing Liu, Shangfeng Liu
{"title":"Light exposure and its applications in human health","authors":"Angze Li, Xiaoling Wei, Yajia Xie, Yi Ren, Xi Zhu, Muqing Liu, Shangfeng Liu","doi":"10.1002/jbio.202400023","DOIUrl":"10.1002/jbio.202400023","url":null,"abstract":"<p>Light exposure has been proven to have a significant impact on human health. As a result, researchers are increasingly exploring its potential benefits and drawbacks. With advancements in understanding light and the manufacturing of light sources, modern health lighting has become widely utilized in daily life and plays a critical role in the prevention and treatment of various illnesses. The use of light in healthcare is a global trend, with many countries actively promoting the development and application of relevant scientific research and medical technology. This field has gained worldwide attention and support from scientists and doctors alike. In this review, we examine the application of lighting in human health and recent breakthroughs in light exposure related to pathology, therapeutic strategies, molecular changes, and more. Finally, we also discuss potential future developments and areas of application.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140570902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"High-resolution cell imaging using white light phase shifting interferometry and iterative phase deconvolution","authors":"Shubham Tiwari, Shilpa Tayal, Shivam Trivedi, Harpreet Kaur, Dalip Singh Mehta","doi":"10.1002/jbio.202300499","DOIUrl":"10.1002/jbio.202300499","url":null,"abstract":"<p>An optimization algorithm is presented for the deconvolution of a complex field to improve the resolution and accuracy of quantitative phase imaging (QPI). A high-resolution phase map can be recovered by solving a constrained optimization problem of deconvolution using a complex gradient operator. The method is demonstrated on phase measurements of samples using a white light based phase shifting interferometry (WLPSI) method. The application of the algorithm on real and simulated objects shows a significant resolution and contrast improvement. Experiments performed on <i>Escherichia coli</i> bacterium have revealed its sub-cellular structures that were not visible in the raw WLPSI images obtained using a five phase shifting method. These features can give valuable insights into the structures and functioning of biological cells. The algorithm is simple in implementation and can be incorporated into other QPI modalities .</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ammonia measurement in human breath of subjects with Helicobacter pylori using photoacoustic spectroscopy","authors":"Cristina Popa, Mioara Petrus, Ana Maria Bratu","doi":"10.1002/jbio.202400074","DOIUrl":"10.1002/jbio.202400074","url":null,"abstract":"<p><i>Helicobacter pylori</i> (<i>H. pylori</i>) is a type of bacteria that infects the stomach. The detection of <i>H. pylori</i> is an essential part of current clinical practices because this disease can cause peptic ulcers, chronic inflammation of the stomach lining but also stomach cancer. <i>Helicobacter pylori</i> has a naturally occurring enzyme that hydrolyzes urea into ammonium carbonate called urease. Many methods exist for the detection of <i>H. pylori</i> infection, but an innovative approach is to detect the ammonia in the breath (ABT, Ammonia Breath Test). In this research study, using photoacoustic spectroscopy method, the ammonia concentration in the breathing zone of people with <i>H. pylori</i> were measured and were compared with ammonia concentration from the respiration of healthy people. From the ABT determinations of this study, the ammonia was established to be increased with 498 ppb at people with <i>H. pylori</i> when we compare with ABT of healthy people.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Synovial fluid research based on SERS and SERRS for enhanced detection of biomarkers in staged osteoarthritis","authors":"Jinjin Wu, Jiachun Dong, Yilin Bao, Linwei Shang, Qingxia Wu, Zichun Yang, Huijie Wang, Jianhua Yin","doi":"10.1002/jbio.202400024","DOIUrl":"10.1002/jbio.202400024","url":null,"abstract":"<p>Surface-enhanced (resonance) Raman scattering (SER(R)S) can extremely enhance Raman intensity of samples, which is helpful for detecting synovial fluid (SF) that does not show Raman activity under normal conditions. In this study, SER(R)S spectra of SF from three different osteoarthritis (OA) stages were collected and analyzed for OA progress, finding that the content of collagen increased throughout the disease, while non-collagen proteins and polysaccharides decreased sharply at advanced OA stage accompanied by the increase of phospholipid. The spectral features and differences were enhanced by salting-out and centrifugation. Much more information on biomolecules at different OA stages was disclosed by using SERRS for the first time, these main trace components (β-carotene, collagen, hyaluronic acid, nucleotide, and phospholipid) can be used as potential biomarkers. It indicates that SERRS has a more comprehensive ability to assist SERS in seeking micro(trace) biomolecules as biomarkers and facilitating accurate and efficient diagnosis and mechanism research of OA.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arash Aghigh, Gaëtan Jargot, Charlotte Zaouter, Samuel E. J. Preston, Melika Saadat Mohammadi, Heide Ibrahim, Sonia V. Del Rincón, Kessen Patten, François Légaré
{"title":"A comparative study of CARE 2D and N2V 2D for tissue-specific denoising in second harmonic generation imaging","authors":"Arash Aghigh, Gaëtan Jargot, Charlotte Zaouter, Samuel E. J. Preston, Melika Saadat Mohammadi, Heide Ibrahim, Sonia V. Del Rincón, Kessen Patten, François Légaré","doi":"10.1002/jbio.202300565","DOIUrl":"10.1002/jbio.202300565","url":null,"abstract":"<p>This study explored the application of deep learning in second harmonic generation (SHG) microscopy, a rapidly growing area. This study focuses on the impact of glycerol concentration on image noise in SHG microscopy and compares two image restoration techniques: Noise-to-Void 2D (N2V 2D, no reference image restoration) and content-aware image restoration (CARE 2D, full reference image restoration). We demonstrated that N2V 2D effectively restored the images affected by high glycerol concentrations. To reduce sample exposure and damage, this study further addresses low-power SHG imaging by reducing the laser power by 70% using deep learning techniques. CARE 2D excels in preserving detailed structures, whereas N2V 2D maintains natural muscle structure. This study highlights the strengths and limitations of these models in specific SHG microscopy applications, offering valuable insights and potential advancements in the field .</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202300565","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The investigation of the molecular changes during lipopolysaccharide-induced systemic inflammation on rat hippocampus by using FTIR spectroscopy","authors":"Gulgun Cakmak-Arslan, Yildiray Kaya, Soner Mamuk, Eyup Sabri Akarsu, Feride Severcan","doi":"10.1002/jbio.202300541","DOIUrl":"10.1002/jbio.202300541","url":null,"abstract":"<p>The aim of this study is to reveal the molecular changes accompanying the neuronal hyper-excitability during lipopolysaccharide (LPS)-induced systemic inflammation on rat hippocampus using Fourier transform infrared (FTIR) spectroscopy. For this aim, the body temperature of Wistar albino rats administered LPS or saline was recorded by radiotelemetry. The animals were decapitated when their body temperature began to decrease by 0.5°C after LPS treatment and the hippocampi of them were examined by FTIR spectroscopy. The results indicated that systemic inflammation caused lipid peroxidation, an increase in the amounts of lipids, proteins and nucleic acids, a decrease in membrane order, an increase in membrane dynamics and changes in the secondary structure of proteins. Principal component analysis successfully separated control and LPS-treated groups. In conclusion, significant structural, compositional and functional alterations occur in the hippocampus during systemic inflammation and these changes may have specific characteristics which can lead to neuronal hyper-excitability.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202300541","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Saliency enhancement method for photoacoustic molecular imaging based on Grüneisen relaxation nonlinear effect","authors":"Xiatian Wang, Zhihua Xie, Riqiang Lin, Chengyou Shu, Shengmiao Lv, Pengkun Guo, Haoxing Xu, Jinke Zhang, Liquan Dong, Xiaojing Gong","doi":"10.1002/jbio.202400004","DOIUrl":"10.1002/jbio.202400004","url":null,"abstract":"<p>Photoacoustic molecular imaging technology has a wide range of applications in biomedical research. In practical scenarios, both the probes and blood generate signals, resulting in the saliency of the probes in the blood environment being diminished, impacting imaging quality. Although several methods have been proposed for saliency enhancement, they inevitably suffer from moderate generality and detection speed. The Grüneisen relaxation (GR) nonlinear effect offers an alternative for enhancing saliency and can improve generality and speed. In this article, the excitation and detection efficiencies are optimized to enhance the GR signal amplitude. Experimental studies show that the saliency of the probe is enhanced. Moreover, the issue of signal aliasing is studied to ensure the accuracy of enhancement results in the tissues. In a word, the feasibility of the GR-based imaging method in saliency enhancement is successfully demonstrated in the study, showing the superiorities of good generality and detection speed.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Brunner, S. H. Unterberger, H. Auer, T. Hautz, S. Schneeberger, R. Stalder, J. Badzoka, C. Kappacher, C. W. Huck, B. Zelger, J. D. Pallua
{"title":"Suitability of Fourier transform infrared microscopy for the diagnosis of cystic echinococcosis in human tissue sections","authors":"A. Brunner, S. H. Unterberger, H. Auer, T. Hautz, S. Schneeberger, R. Stalder, J. Badzoka, C. Kappacher, C. W. Huck, B. Zelger, J. D. Pallua","doi":"10.1002/jbio.202300513","DOIUrl":"10.1002/jbio.202300513","url":null,"abstract":"<p>Cystic echinococcosis (CE) is a global health concern caused by cestodes, posing diagnostic challenges due to nonspecific symptoms and inconclusive radiographic results. Diagnosis relies on histopathological evaluation of affected tissue, demanding comprehensive tools. In this retrospective case study, Fourier transform infrared microscopy was explored for detecting and identifying CE through biochemical changes in human tissue sections. Tissue samples from 11 confirmed CE patients were analyzed. Archived FFPE blocks were cut and stained, and then CE-positive unstained sections were examined using Fourier transform infrared microscopy post-deparaffinization. Results revealed the method's ability to distinguish echinococcus elements from human tissue, irrespective of organ type. This research showcases the potential of mid-infrared microscopy as a valuable diagnostic tool for CE, offering promise in enhancing diagnostic precision in the face of the disease's complexities.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202300513","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}