Christian A. Iriarte-Valdez, Johannes Wenzel, Emilie Baron, Alexandra Y. Claus, Stefan Kalies, Karsten Sperlich, Oliver Stachs, Maria Leilani Torres-Mapa, Alexander Heisterkamp
{"title":"Assessing UVA and Laser-Induced Crosslinking via Brillouin Microscopy","authors":"Christian A. Iriarte-Valdez, Johannes Wenzel, Emilie Baron, Alexandra Y. Claus, Stefan Kalies, Karsten Sperlich, Oliver Stachs, Maria Leilani Torres-Mapa, Alexander Heisterkamp","doi":"10.1002/jbio.202400401","DOIUrl":"10.1002/jbio.202400401","url":null,"abstract":"<p>Keratoconus and other corneal ectatic disorders involve the degradation of collagen fibers, which compromises the corneal biomechanical properties. Ultraviolet-A (UVA) crosslinking has emerged as the primary treatment to slow down collagen degradation. This treatment is limited in both penetration depth and spatial precision, potentially leading to unwanted side effects. This study compares the changes in biomechanical properties of corneas crosslinked with UVA irradiation and a near-infrared femtosecond laser, using Brillouin microscopy. The biomechanical properties of the crosslinked regions were mapped in terms of Brillouin frequency shift in three dimensions. UVA crosslinking showed an average increase in Brillouin frequency shift of ~100 MHz. We demonstrate targeted spatial and axial corneal femtosecond crosslinking, with similar Brillouin frequency shift values to UVA in crosslinked regions.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400401","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jianming Yu, Yuchen He, Bin Li, Hui Chen, Huaibin Zheng, Jianbin Liu, Lifan Zhou, Yi Niu, Haodong Wu, Zhuo Xu
{"title":"Non-Contact Heart Rate Measurement Using Ghost Imaging System","authors":"Jianming Yu, Yuchen He, Bin Li, Hui Chen, Huaibin Zheng, Jianbin Liu, Lifan Zhou, Yi Niu, Haodong Wu, Zhuo Xu","doi":"10.1002/jbio.202400517","DOIUrl":"10.1002/jbio.202400517","url":null,"abstract":"<div>\u0000 \u0000 <p>Remote heart rate measurement is an increasingly concerned research field, usually using remote photoplethysmography to collect heart rate information through video data collection. However, in certain specific scenarios (such as low light conditions, intense lighting, and non-line-of-sight situations), traditional methods fail to capture image information effectively, that may lead to difficulty or inability in measuring heart rate. To address these limitations, this study proposes non-contact heart rate detection based on ghost imaging architecture. The mean absolute error between experimental measurements and reference true values is 4.24 bpm. Additionally, the bucket signals obtained by the ghost imaging system can be directly processed using digital signal processing techniques, thereby enhancing personal privacy protection.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Priyanka A. Jadhav, Arti Hole, Panchali Saha, Kshama Pansare, Aishwarya Ghanwat, Poonam Gera, Kiran Bendale, C. Murali Krishna, Pradip Chaudhari
{"title":"Exploratory Raman Spectroscopic Studies of Canine Oral Tumour Types","authors":"Priyanka A. Jadhav, Arti Hole, Panchali Saha, Kshama Pansare, Aishwarya Ghanwat, Poonam Gera, Kiran Bendale, C. Murali Krishna, Pradip Chaudhari","doi":"10.1002/jbio.202400372","DOIUrl":"10.1002/jbio.202400372","url":null,"abstract":"<div>\u0000 \u0000 <p>Canine cancers are becoming increasingly significant due to their natural occurrence, similar to the spontaneous cancers in humans as well as their histological and biological similarities to human cancers. Several oral cancer types have been witnessed in dogs, based on the cell type from which the tumour originates. The type of oral tumour dictates severity of the disease, treatment options and prognoses. The current tissue-based Raman Spectroscopy (RS) study explores stratification of canine cancers. Raman spectra of histopathologically confirmed normal and oral tumour types namely Epulis, Spindle cell sarcoma (SCS), and Squamous cell carcinoma (SCC) were acquired using a Raman confocal microscope with 532 nm laser, pre-processed and multivariate analyses performed. PC-LDA achieved overall classification accuracy of > 70%. Thus, the present study evaluates potential of RS to identify the tumour type based on the identification of characteristic spectral features. Findings warrant large scale in vivo RS explorations in canine cancer subjects.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
He Gao, Yao Liu, Zhaiyi Liu, Pengquan Wang, Zhaoyin Qin, Shuanglin Liao, Jia Mo, Lin Wang, Hsiang-Chen Chui
{"title":"Enhanced Hair Regrowth Through Dual-Wavelength Low-Level Laser Therapy: A Comparative Study on Mice","authors":"He Gao, Yao Liu, Zhaiyi Liu, Pengquan Wang, Zhaoyin Qin, Shuanglin Liao, Jia Mo, Lin Wang, Hsiang-Chen Chui","doi":"10.1002/jbio.202400523","DOIUrl":"10.1002/jbio.202400523","url":null,"abstract":"<div>\u0000 \u0000 <p>Low-level laser therapy (LLLT) is a non-invasive treatment that uses low-power lasers or light sources to stimulate biological responses. Previous studies focused on single-wavelength effects on hair growth in mice. In this study, we investigated dual-wavelength combinations to enhance hair regrowth. Using light-emitting diodes (LEDs) at 670, 680, 780, and 880 nm, we assessed the correlation between different wavelength pairs and hair regrowth after shaving in mice. Phototherapy was conducted over 14 days, with results analyzed through surface image analysis and histological examination. Our findings indicate that dual-wavelength therapy is superior to single-wavelength treatment and significantly more effective than the untreated control, demonstrating its potential for improved hair regrowth.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jilan S. Ibrahim, Neamat Hanafi, Mahmoud A. Sliem, Tarek A. El-Tayeb
{"title":"Enhanced Photothermal Tumor Ablation Using Polypyrrole-Gold Nanocomposites Activated by Polarized Polychromatic Low-Energy Light: An In Vivo Study","authors":"Jilan S. Ibrahim, Neamat Hanafi, Mahmoud A. Sliem, Tarek A. El-Tayeb","doi":"10.1002/jbio.202400488","DOIUrl":"10.1002/jbio.202400488","url":null,"abstract":"<div>\u0000 \u0000 <p>Photothermal therapy (PTT) offers a minimally invasive approach for cancer treatment, using light energy to selectively heat and destroy cancer cells. Success in PTT depends on efficient, stable, and biocompatible photothermal agents. This study investigates polypyrrole@gold nanocomposites (PPy@Au NCs) as photothermal agents combined with polarized polychromatic low-energy light (PPLEL) to target tumors and limit disease progression. In vivo experiments on Ehrlich carcinoma-bearing female Swiss albino mice demonstrated that PPy@Au NCs selectively accumulated in tumor tissue and, when activated by PPLEL, generated sufficient heat for effective tumor ablation. This approach enhanced treatment efficacy and presented a cost-effective solution due to the affordability of both the nanocomposite and light source. Histopathological analysis confirmed significant tumor reduction, suggesting that this synergistic combination offers a promising cancer treatment strategy. Findings support further research and potential clinical applications in photothermal cancer therapy.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lyudmila A. Bratchenko, Yulia A. Khristoforova, Irina A. Pimenova, Mikhail S. Snegerev, Vitaly I. Kupaev, Petr A. Lebedev, Yuri V. Kistenev, Ivan A. Bratchenko
{"title":"Comparative Study Into the Effect of Detector Noises and Sensitivity on the Serum SERS Analysis: Example of Non-Communicable Diseases Discrimination","authors":"Lyudmila A. Bratchenko, Yulia A. Khristoforova, Irina A. Pimenova, Mikhail S. Snegerev, Vitaly I. Kupaev, Petr A. Lebedev, Yuri V. Kistenev, Ivan A. Bratchenko","doi":"10.1002/jbio.202400475","DOIUrl":"10.1002/jbio.202400475","url":null,"abstract":"<div>\u0000 \u0000 <p>The aim of the study is to compare the performance of surface-enhanced Raman spectroscopy (SERS) analysis of serum using a non-cooled detector (<i>EnSpectr R785</i>) and a high spectral resolution detector (<i>Renishaw</i>) in the task of discrimination between the patients with chronic heart failure and the patients with chronic obstructive pulmonary disease. The SERS-based solution of the classification problem demonstrates an insignificant relationship between the disease classification accuracy and detector quality (classification accuracy for the high-resolution setup is 0.84 and for the low-cost setup is 0.81). In the data recorded on <i>Renishaw</i> setup, the most significant bands are 611, 675, 720, 804, 1187, 1495, and 1847 cm<sup>−1</sup>; for the <i>EnSpectr</i> setup—721, 1051, and 1665 cm<sup>−1</sup>. The results have revealed equal discrimination capabilities in the <i>EnSpectr</i> and the <i>Renishaw</i> setups; however, the <i>Renishaw</i> setup has more prospects for identifying the spectral contribution of pathologically associated analytes.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Da Sun, Bingchen Yu, Rui Xu, Chenzheng Wang, Kai Pang
{"title":"Laser-Induced Minimization of Circulating Tumor Cells to Suppress Tumor Metastasis","authors":"Da Sun, Bingchen Yu, Rui Xu, Chenzheng Wang, Kai Pang","doi":"10.1002/jbio.202400569","DOIUrl":"10.1002/jbio.202400569","url":null,"abstract":"<div>\u0000 \u0000 <p>Circulating tumor cells (CTCs) are key indicators of tumor metastasis. Effective clearance of CTCs can reduce the probability of metastasis. We designed a system for the real-time dynamic monitoring and clearance of CTCs, capable of monitoring and clearing CTCs in the living circulatory system. Experimental results showed that pulsed laser treatment significantly affects the clearance of melanoma CTCs. Through in vivo imaging of small animals and survival analysis of mice, we observed that CTC clearance could reduce the size of distant metastatic lesions and prolong the lifespan of the mice. Additionally, we set up a hemolysis experiment to demonstrate that the laser energy used does not cause damage to red blood cells. This study is based on the physical and mechanical destruction of CTCs, meaning there is no issue of drug resistance. This provides a novel approach and technical means for suppressing tumor metastasis and extending the lifespan of patients in clinical settings.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fast In Vivo Two-Photon Fluorescence Imaging via Lateral and Axial Resolution Restoration With Self-Supervised Learning","authors":"Zhengyuan Pan, Man Lei, Hongen Liao, Bobo Gu","doi":"10.1002/jbio.202400489","DOIUrl":"10.1002/jbio.202400489","url":null,"abstract":"<div>\u0000 \u0000 <p>Two-photon fluorescence (TPF) imaging opens a new avenue to achieve high resolution at extended penetration depths. However, it is difficult for conventional TPF imaging systems to simultaneously achieve high resolution and speed. In this work, we develop an innovative deep learning framework of Lateral and Axial Resolution Restoration (LARR) to break the contradiction between imaging resolution and speed. LARR employs a self-supervised training scheme to computationally restore the sparsely sampled TPF images to resolution isotropic images by 4-fold axial and 16-fold lateral resolution enhancement. The simulation studies and experimental results demonstrate the excellent performance of LARR to preserve fine structural features with improved signal-to-noise ratio and structure similarity index. Moreover, the TPF imaging system with the LARR is able to achieve 60-fold improved imaging speed and comparable resolution as compared with the conventional TPF system. The outstanding performance makes LARR a potential tool for fast TPF imaging with high resolution.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laura Perego, Caterina Dallari, Chiara Falciani, Alessandro Pini, Lucia Gardini, Caterina Credi, Francesco Saverio Pavone
{"title":"A Compact Prism-Based Microscope for Highly Sensitive Measurements in Fluid Biopsy","authors":"Laura Perego, Caterina Dallari, Chiara Falciani, Alessandro Pini, Lucia Gardini, Caterina Credi, Francesco Saverio Pavone","doi":"10.1002/jbio.202400519","DOIUrl":"10.1002/jbio.202400519","url":null,"abstract":"<p>The increasing demand for sensitive, portable, and affordable disease detection methods has spurred the development of advanced biosensors for rapid early-stage diagnosis, population mass screening, and bed-monitoring. Current high-sensitivity devices face hurdles such as high production costs and challenges in multiplexed signal detection. To address these, we developed a prism-based total internal reflection system which, in combination with surface functionalization techniques of gold nanoparticles, enables evanescent wave scattering for highly sensitive and rapid detection of specific analytes in both synthetic and human liquid samples. To validate its efficacy, we conducted scattering experiments in synthetic and human serum samples, exploiting functionalized AuNPs to recognize bacterial lipopolysaccharides as biomarkers for sepsis disease. We demonstrate a remarkable sensitivity in the femtogram per mL concentration range for this specific pathological biomarker. Based on this result we envisage the potential adoption of our technique for liquid biopsy in the clinical scenario.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400519","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction of Non-Uniform Rotational Distortion in the Proximally Controlled Endoscopic OCTA","authors":"Zehua Guan, Qiang Li, Chen Niu, Shuhao Fan, Huanhuan Yu, Wenjuan Wu, Xiangfei Feng, Cuixia Dai","doi":"10.1002/jbio.202400467","DOIUrl":"10.1002/jbio.202400467","url":null,"abstract":"<div>\u0000 \u0000 <p>Endoscopic Optical Coherence Tomography (OCT) can provide high-resolution cross-sectional images for internal organ tissues. Combining the endoscopic imaging with Optical Coherence Tomography Angiography (OCTA), information of blood vessels in superficial lumen tissues is expected to be acquired. However, in endoscopic OCT systems using proximal scanning probes, performance of OCTA encounters significant challenges due to non-uniform rotational distortion (NURD) caused by the non-constant rotation of the distal imaging unit. In this study, we proposed a registration method for endoscopic OCTA imaging in a proximally controlled OCT System. Global registration and A-line registration were employed to correct the distortion caused by mechanical friction between the catheter sheath and torque coil. Experimental performances in both microfluidic channel and rat rectum show significant correction of NURD. Our study achieved the first implementation of endoscopic OCTA under a spiral B-scan rotation scheme in a proximally controlled OCT system, facilitating clear visualization of blood flow.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}