Journal of Biophotonics最新文献_第10页

Quantitative Detection of Orthotopic Liver Cancer in Mice Using Indocyanine Green and Dynamic Diffuse Fluorescence Tomography Imaging 吲哚菁绿和动态漫射荧光断层成像定量检测小鼠原位肝癌。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.70003

Zhuanxia He, Limin Zhang, Zishuo Li, Xiujun Gao, Yanqi Zhang, Feng Gao

{"title":"Quantitative Detection of Orthotopic Liver Cancer in Mice Using Indocyanine Green and Dynamic Diffuse Fluorescence Tomography Imaging","authors":"Zhuanxia He, Limin Zhang, Zishuo Li, Xiujun Gao, Yanqi Zhang, Feng Gao","doi":"10.1002/jbio.70003","DOIUrl":"10.1002/jbio.70003","url":null,"abstract":"<div>\u0000 \u0000 <p>Orthotopic tumor model has become an essential tool for studying drug biodistribution and tumor progression over time owing to the rapid development of in vivo imaging and immunological science. Dynamic diffuse fluorescence tomography (DFT) is a promising imaging modality that can map the three-dimensional distribution of a fluorophore within the object and capture the metabolic parameters of fluorophores in vivo. It has been widely applied in tumor detection, drug development, and efficacy evaluation. To detect orthotopic liver tumors, we combined indocyanine green (ICG) and a DFT system to perform fluorescence imaging and quantitative analysis for orthotopic liver tumors in mice. The orthotopic liver models were first established, and the liver fluorescence yields were detected in pre- and post-cancerous liver using the DFT system. The results showed that there was higher liver uptake and prolonged retention in orthotopic tumor liver compared to normal liver. Furthermore, the pharmacokinetic parameters suggested the uptake coefficient of tumor liver was above twice that of normal liver, while the excretion rates were similar. Additionally, to intuitively assess tumor occurrence, we propose using normalized fluorescence ratios of 5 and maximum fluorescence values of 0.01 mm<sup>−1</sup> as evaluation criteria. The study demonstrates DFT imaging is a promising tool in orthotopic tumor model detection and drug or agent metabolic evaluation.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Compact Monocular Video-Ophthalmoscope to Measure Retinal Reflectance Changes to Flicker Light Stimuli 紧凑型单眼视频检眼镜测量视网膜对闪烁光刺激的反射变化。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.202400494

Radim Kolar, Tomas Vicar, Jiri Chmelik, Roman Jakubicek, Jan Odstrcilik, Michal Nohel, Karolina Skorkovska, Ralf P. Tornow

{"title":"Compact Monocular Video-Ophthalmoscope to Measure Retinal Reflectance Changes to Flicker Light Stimuli","authors":"Radim Kolar, Tomas Vicar, Jiri Chmelik, Roman Jakubicek, Jan Odstrcilik, Michal Nohel, Karolina Skorkovska, Ralf P. Tornow","doi":"10.1002/jbio.202400494","DOIUrl":"10.1002/jbio.202400494","url":null,"abstract":"<p>This paper describes a compact video-ophthalmoscope (VO) designed for capturing retinal video sequences of the optic nerve head (ONH) under flicker light stimulation. The device uses an OLED display and a fiber optic-coupled LED light source, enabling high-frame-rate video at low illumination intensity (12 μW/cm<sup>2</sup>). Retinal responses were recorded in 10 healthy subjects during flicker light exposure with a pupil irradiance of 2 μW/cm<sup>2</sup>. Following 20 s of stimulation, all subjects displayed changes in retinal reflectance and pulsation attenuation, linked to blood flow and volume variations. These findings suggest that increased blood volume leads to decreased retinal reflectance. Temporal analysis confirmed the ability to capture flicker-induced retinal reflectance changes, indicating its potential for spatial and temporal analysis. Overall, this device offers a portable approach for investigating dynamic retinal responses to light stimuli, which can aid the diagnosis of retinal diseases like diabetic retinopathy, glaucoma, or neurodegenerative diseases affecting retinal blood circulation.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensing Dog Brain Reactions to Smell by AI Speckle Pattern Analysis 通过人工智能斑点模式分析感知狗的大脑对气味的反应。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.202400501

Ilana Propp, Marianna Beiderman, Sergey Agdarov, Yafim Beiderman, Maxi Blum, Zeev Zalevsky

引用次数: 0

Noncontact Detection of Blood Coagulation Dynamics Based on Speckle Deviation Analysis Using Optical Coherence Tomography 基于光学相干断层成像散斑偏差分析的非接触血液凝固动力学检测。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.70002

Yun Tang, Zongqing Ma, Yuxian Zhang, Fan Fan, Fan Zhang, Jiang Zhu

{"title":"Noncontact Detection of Blood Coagulation Dynamics Based on Speckle Deviation Analysis Using Optical Coherence Tomography","authors":"Yun Tang, Zongqing Ma, Yuxian Zhang, Fan Fan, Fan Zhang, Jiang Zhu","doi":"10.1002/jbio.70002","DOIUrl":"10.1002/jbio.70002","url":null,"abstract":"<div>\u0000 \u0000 <p>Red blood cells aggregate from individual cells into larger aggregates during blood coagulation. This process causes the light-scattering particles to enlarge and their motion to become restricted. The size and motion of these light-scattering particles during coagulation provide valuable information on the progress of blood coagulation. This paper proposes an optical coherence tomography speckle deviation (OCT-SD) method for the noncontact assessment of blood coagulation dynamics by detecting changes in the size and motion of scattering particles. The coagulation characteristics of static and flowing blood were quantitatively evaluated through coagulation reaction time and clot formation duration by monitoring the blood coagulation process. The OCT imaging, incorporating SD analysis, enables noncontact coagulation assessment. Experimental results demonstrate that the proposed method can efficiently and accurately assess coagulation dynamics, offering significant potential for in situ coagulation detection.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confocal Laser Scanning Platform Combined With In Situ High-Resolution Quantitative Phase Imaging 结合原位高分辨率定量相位成像的共聚焦激光扫描平台。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-07 DOI: 10.1002/jbio.202500010

Wenjing Feng, Hongfei Suo, Ying Ma, Hanwei Cheng, Peng Gao, Yunze Lei, Sha An, Juanjuan Zheng, Min Liu

{"title":"Confocal Laser Scanning Platform Combined With In Situ High-Resolution Quantitative Phase Imaging","authors":"Wenjing Feng, Hongfei Suo, Ying Ma, Hanwei Cheng, Peng Gao, Yunze Lei, Sha An, Juanjuan Zheng, Min Liu","doi":"10.1002/jbio.202500010","DOIUrl":"10.1002/jbio.202500010","url":null,"abstract":"<div>\u0000 \u0000 <p>This paper introduces an advanced dual-modality imaging platform by integrating a confocal laser scanning/imaging module with flat-fielding quantitative phase contrast microscopy (FF-QPCM). The platform enables phase/fluorescence dual-modality imaging of the same samples with high resolution and contrast. Using this platform, we observed that confocal fluorescence excitation causes minimal damage to cells, whereas wide-field fluorescence excitation (WFE) results in significant damage, particularly mitochondrial fragmentation and notable alterations in phase values. Additionally, in situ quantitative phase imaging was conducted on live COS7 cells with a specific region irradiated by a confocal laser. The results demonstrated that 5-min continuous confocal laser irradiation affects only COS7 cells with fluorescently labeled mitochondria, causing mitochondrial dysfunction throughout the cell. These findings suggest that integrating FF-QPCM with a confocal laser scanning/imaging module offers a highly efficient, sensitive, and information-rich tool for cell biology research, with broad application potential.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Full-Field Assessment of Geometry and Collagenous Architecture of Porcine Valve Leaflets via Laser Micrometry and Quantitative Polarized Light Imaging 利用激光显微测量和定量偏振光成像技术对猪瓣膜小叶的几何结构和胶原结构进行全方位评估。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-06 DOI: 10.1002/jbio.202400511

Shreya Sreedhar, Daniel P. Pearce, Colleen M. Witzenburg

{"title":"Full-Field Assessment of Geometry and Collagenous Architecture of Porcine Valve Leaflets via Laser Micrometry and Quantitative Polarized Light Imaging","authors":"Shreya Sreedhar, Daniel P. Pearce, Colleen M. Witzenburg","doi":"10.1002/jbio.202400511","DOIUrl":"10.1002/jbio.202400511","url":null,"abstract":"<p>Despite the frequent failure of aortic valves and pediatric usage of pulmonary valves as a replacement, comparative studies on their full-field collagenous architecture and macroscale geometries are limited. We applied laser micrometry and quantitative polarized light imaging (QPLI), a novel technique for assessing collagen fiber organization, to porcine aortic (<i>n</i> = 8) and pulmonary (<i>n</i> = 8) valve leaflets to non-destructively compare thickness and anisotropy. We confirmed (1) light intensity and sample thickness are inversely related and (2) aortic valve leaflets are thicker with decreased fiber organization when unloaded. To demonstrate the ability of QPLI to capture dynamic collagen fiber alignment, we imaged leaflets during equibiaxial loading. There was an increase in the aortic leaflet's degree of alignment throughout loading, whereas the pulmonary valve leaflet exhibited relatively unchanged alignment. Ultimately, understanding the full-field organization of a leaflet's heterogeneous ECM and how it is altered by pathology can inform therapy development.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400511","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiation of Bacterial Species in Liquid Culture Using Laser Speckle Contrast Imaging 激光散斑对比成像技术在液体培养细菌种类鉴别中的应用。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-05 DOI: 10.1002/jbio.202400565

Priya Krishnamurthy, Roshni Shetty, Kiran Philip Isaac, Sujatha Narayanan Unni, Sudhanarayani S Rao, Krupakar Parthasarathy

{"title":"Differentiation of Bacterial Species in Liquid Culture Using Laser Speckle Contrast Imaging","authors":"Priya Krishnamurthy, Roshni Shetty, Kiran Philip Isaac, Sujatha Narayanan Unni, Sudhanarayani S Rao, Krupakar Parthasarathy","doi":"10.1002/jbio.202400565","DOIUrl":"10.1002/jbio.202400565","url":null,"abstract":"<div>\u0000 \u0000 <p>Bacterial identification is vital for healthcare and environmental quality control. Traditional bacterial identification methods require extensive sample preparation, including cultivation, staining, and microscopy, making them time-consuming and labor-intensive. This study proposes the application of Laser Speckle Contrast Imaging (LSCI) as a novel approach to capture variations in speckle patterns between the start and end of the lag caused by changes in the shape and arrangement of bacterial cells during cell division in liquid cultures at lower cell concentrations, such as in the lag phase. Our approach offers an efficient alternative to traditional methods of bacterial species identification demonstrated with \u0000 <i>Escherichia coli</i>\u0000 and \u0000 <i>Micrococcus luteus</i>\u0000 pairs. Also, the differentiation of strains (\u0000 <i>E. coli</i>\u0000 ATCC25922 and DH5α) is carried out based on the percentage change in speckle contrast between the end of lag and mid-log phase of their growth curve.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143560486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-Free Multiphoton Microscopy for Diagnosis of Psoriasis 无标记多光子显微镜诊断银屑病。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-05 DOI: 10.1002/jbio.202500025

Liqin Zheng, Liuzhi Wu, Wenwen Zhong, Shengrong Du, Zhen Li, Xi Chen, Jianping Huang, Shulian Wu, Lina Liu, Jianxin Chen, Yelin Wu

引用次数: 0

Visible Blue Light Does Not Induce DNA Damage in Human Dermal Fibroblasts 可见蓝光不会诱导人皮肤成纤维细胞DNA损伤。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-04 DOI: 10.1002/jbio.202400510

Margaret Kabakova, Jennifer Wang, Julia Stolyar, Evan Austin, Jared Jagdeo

引用次数: 0

Effect of Sequential Repetitive Transcranial Magnetic Stimulation With Bilateral Arm Training on the Brain Effective Connectivity in Chronic Stroke 连续重复经颅磁刺激联合双侧手臂训练对慢性脑卒中患者脑有效连通性的影响。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-04 DOI: 10.1002/jbio.202400508

Wenhao Li, Ping Cao, Ran Wei, Duo Wai-Chi Wong

{"title":"Effect of Sequential Repetitive Transcranial Magnetic Stimulation With Bilateral Arm Training on the Brain Effective Connectivity in Chronic Stroke","authors":"Wenhao Li, Ping Cao, Ran Wei, Duo Wai-Chi Wong","doi":"10.1002/jbio.202400508","DOIUrl":"10.1002/jbio.202400508","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigated the effects of combining repetitive transcranial magnetic stimulation (rTMS) with bilateral arm training (BAT) on effective brain connectivity in chronic stroke patients using functional near-infrared spectroscopy. Fifteen post-stroke patients and fifteen healthy individuals were enrolled. Coupling function analysis was performed to evaluate the effective connectivity inflow, outflow, and the dominant information flow (DIF) during standalone BAT and combined rTMS-BAT therapy. Significant task-related alterations were observed in the ipsilesional supplementary motor area and occipital lobe (OL) of stroke patients undergoing rTMS-BAT. During BAT, stroke patients exhibited more pronounced DIF from the OL to motor areas compared to healthy controls. Furthermore, in the rTMS-BAT condition, patients demonstrated enhanced DIF from the ipsilesional OL and contralesional prefrontal cortex to ipsilesional motor areas. These findings suggested a potential synergistic effect on cortical reorganization through the sequential combination of task-related training and TMS in chronic stroke patients, offering insights into rehabilitation strategies.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0