Label-Free Multiphoton Microscopy for Diagnosis of Psoriasis.

Liqin Zheng, Liuzhi Wu, Wenwen Zhong, Shengrong Du, Zhen Li, Xi Chen, Jianping Huang, Shulian Wu, Lina Liu, Jianxin Chen, Yelin Wu
{"title":"Label-Free Multiphoton Microscopy for Diagnosis of Psoriasis.","authors":"Liqin Zheng, Liuzhi Wu, Wenwen Zhong, Shengrong Du, Zhen Li, Xi Chen, Jianping Huang, Shulian Wu, Lina Liu, Jianxin Chen, Yelin Wu","doi":"10.1002/jbio.202500025","DOIUrl":null,"url":null,"abstract":"<p><p>Psoriasis is a global problem that significantly affects patients both physically and mentally. Accurate and rapid detection of psoriasis is crucial for diagnosis and treatment. Herein, we utilized multiphoton microscopy (MPM) to rapidly image psoriasis in imiquimod-induced mouse and human psoriatic samples. Our results showed that MPM accurately monitored the thickening of stratum corneum and epidermis in psoriatic tissues, similar to traditional H&E staining. Notably, we observed an increased alignment and number of collagen fibers in both mouse models and human psoriatic samples. Furthermore, a decrease in the proportionate area, length, width, and cross-linking gap of collagen fibers, as well as an increase in cross-linking density was also obtained in mouse psoriatic models. These findings suggest that collagen can be a biomarker for evaluating the progress of psoriasis. Overall, our study presents a rapid diagnostic method for psoriasis using MPM.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500025"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biophotonics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/jbio.202500025","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Psoriasis is a global problem that significantly affects patients both physically and mentally. Accurate and rapid detection of psoriasis is crucial for diagnosis and treatment. Herein, we utilized multiphoton microscopy (MPM) to rapidly image psoriasis in imiquimod-induced mouse and human psoriatic samples. Our results showed that MPM accurately monitored the thickening of stratum corneum and epidermis in psoriatic tissues, similar to traditional H&E staining. Notably, we observed an increased alignment and number of collagen fibers in both mouse models and human psoriatic samples. Furthermore, a decrease in the proportionate area, length, width, and cross-linking gap of collagen fibers, as well as an increase in cross-linking density was also obtained in mouse psoriatic models. These findings suggest that collagen can be a biomarker for evaluating the progress of psoriasis. Overall, our study presents a rapid diagnostic method for psoriasis using MPM.

求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信