Label-Free Multiphoton Microscopy for Diagnosis of Psoriasis

IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS
Liqin Zheng, Liuzhi Wu, Wenwen Zhong, Shengrong Du, Zhen Li, Xi Chen, Jianping Huang, Shulian Wu, Lina Liu, Jianxin Chen, Yelin Wu
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引用次数: 0

Abstract

Psoriasis is a global problem that significantly affects patients both physically and mentally. Accurate and rapid detection of psoriasis is crucial for diagnosis and treatment. Herein, we utilized multiphoton microscopy (MPM) to rapidly image psoriasis in imiquimod-induced mouse and human psoriatic samples. Our results showed that MPM accurately monitored the thickening of stratum corneum and epidermis in psoriatic tissues, similar to traditional H&E staining. Notably, we observed an increased alignment and number of collagen fibers in both mouse models and human psoriatic samples. Furthermore, a decrease in the proportionate area, length, width, and cross-linking gap of collagen fibers, as well as an increase in cross-linking density was also obtained in mouse psoriatic models. These findings suggest that collagen can be a biomarker for evaluating the progress of psoriasis. Overall, our study presents a rapid diagnostic method for psoriasis using MPM.

Abstract Image

无标记多光子显微镜诊断银屑病。
牛皮癣是一个全球性的问题,严重影响患者的身体和精神。准确、快速的检测牛皮癣是诊断和治疗的关键。在此,我们利用多光子显微镜(MPM)对吡喹莫德诱导的小鼠和人银屑病样品进行快速成像。结果表明,MPM能准确监测银屑病组织角质层和表皮的增厚,与传统的H&E染色相似。值得注意的是,我们在小鼠模型和人类银屑病样本中观察到胶原纤维的排列和数量增加。此外,在小鼠银屑病模型中,胶原纤维的比例面积、长度、宽度和交联间隙减少,交联密度增加。这些发现表明胶原蛋白可以作为评估牛皮癣进展的生物标志物。总之,我们的研究提出了一种使用MPM快速诊断银屑病的方法。
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来源期刊
Journal of Biophotonics
Journal of Biophotonics 生物-生化研究方法
CiteScore
5.70
自引率
7.10%
发文量
248
审稿时长
1 months
期刊介绍: The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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