Journal of Biophotonics最新文献_第6页

Experimental Study on the Process and Performance of Laser Repair of Esophageal Tissue Adding ICG and CNT 添加ICG和碳纳米管激光修复食管组织的过程和性能的实验研究。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-07-23 DOI: 10.1002/jbio.70324

Jun Huang, Xijing Yan, Hui Jiang, Mintao Yan, Yanyu Li, Jincheng Li, Kehong Wang

引用次数: 0

Multi-Class and Multi-Level Classification for Common Wound Bacteria Based on Reflectance Hyperspectral Imaging 基于反射率高光谱成像的常见伤口细菌多类别多层次分类。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-07-23 DOI: 10.1002/jbio.70318

Decheng Wu, Xudong Fu, Wendan Liu, Sheng Yin, Chang Liu, Jiaqi Zhao, Hailin Cao, Lianyang Zhang, Zhen Wang, Hao Tang

{"title":"Multi-Class and Multi-Level Classification for Common Wound Bacteria Based on Reflectance Hyperspectral Imaging","authors":"Decheng Wu, Xudong Fu, Wendan Liu, Sheng Yin, Chang Liu, Jiaqi Zhao, Hailin Cao, Lianyang Zhang, Zhen Wang, Hao Tang","doi":"10.1002/jbio.70318","DOIUrl":"10.1002/jbio.70318","url":null,"abstract":"<div>\u0000 \u0000 <p>Hyperspectral imaging is an emerging non-destructive technique for rapid bacterial detection. However, existing methods often inadequately exploit spatial–spectral correlations and lack datasets tailored to clinically relevant scenarios. This work presents a systematic framework integrating a near-infrared hyperspectral imaging system with a deep learning for accurate classification of common wound bacteria. The multi-class and multi-level dataset is first established using a 400–2500 nm halogen-illuminated system under controlled conditions, comprising 26 056 image patches covering seven bacterial species at five concentration levels, totaling 31 classes. To fully harness spatial and spectral information, the Spatial–Spectral Interactive Selection Network (SSIS-Net) is proposed. Evaluated on the self-constructed dataset, SSIS-Net achieves an overall accuracy of 97.40%, an average accuracy of 97.37%, and a Kappa coefficient of 97.31%, consistently outperforming state-of-the-art methods. These results demonstrate that the proposed system offers a reliable, rapid, and non-destructive solution for bacterial detection, with strong potential for clinical integration.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148581970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imaging Transmembrane Kinetics: An Asymmetric Phthalocyanine Enables Fast Cellular Entry and Golgi Targeting for Enhanced Photodynamic Therapy 成像跨膜动力学：不对称酞菁使细胞快速进入和高尔基靶向增强光动力治疗。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-07-08 DOI: 10.1002/jbio.70322

Bingyang Liu, Yuanyuan Han, Kuizhi Chen, Guowei Liu, Jianling Chen, Kun Wang, Yiru Peng, Hongqin Yang

{"title":"Imaging Transmembrane Kinetics: An Asymmetric Phthalocyanine Enables Fast Cellular Entry and Golgi Targeting for Enhanced Photodynamic Therapy","authors":"Bingyang Liu, Yuanyuan Han, Kuizhi Chen, Guowei Liu, Jianling Chen, Kun Wang, Yiru Peng, Hongqin Yang","doi":"10.1002/jbio.70322","DOIUrl":"10.1002/jbio.70322","url":null,"abstract":"<div>\u0000 \u0000 <p>Photodynamic therapy (PDT) is fundamentally limited by the difficulty of concurrently achieving efficient cellular uptake and precise organelle-specific localization of photosensitizers (PSs). To address this challenge, we designed an asymmetric silicon phthalocyanine, Chol–SiPc–PIP, through axial conjugation with cholesterol and piperazine groups, aiming to optimize its cellular pharmacokinetics. Combining advanced optical microscopy techniques, we investigated the cellular behavior of Chol–SiPc–PIP at high spatiotemporal resolution. Two-photon CLSM showed rapid uptake and Golgi accumulation in MCF-7 cells, while FCS revealed that the piperazine moiety accelerates membrane translocation, driving fast cellular entry. Benefiting from enhanced cellular uptake kinetics, precise Golgi targeting, and favorable photophysical properties, Chol–SiPc–PIP demonstrates superior in vitro photodynamic performance, showing an IC<sub>50</sub> value as low as 98 nM with excellent biocompatibility. This study highlights how asymmetric molecules can be strategically engineered to control subcellular pharmacokinetics and organelle-level photodynamic activity, offering a cell-focused design paradigm for developing advanced PSs with translational potential.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148414478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward Clinical Translation of a Bimodal Fluorescence Tool for Oral Cancer Detection and Biopsy Guidance 用于口腔癌检测和活检指导的双峰荧光工具的临床翻译。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-07-07 DOI: 10.1002/jbio.70321

Nemichand, Bhaswati Singha Deo, Shivam Shukla, Rachna Rath, Asima Pradhan

引用次数: 0

Evaluation of the Usefulness of Machine Learning and Artificial Intelligence on Hyperspectral Images in the Diagnosis of Myelodysplastic Syndrome 评估机器学习和人工智能在高光谱图像诊断骨髓增生异常综合征中的有用性。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-07-06 DOI: 10.1002/jbio.70319

Yasuo Ueda, Takafumi Ogawa, Akane Wada, Genshu Tate, Yuta Baba, Tetsuya Fukuda, Toshiko Yamochi

{"title":"Evaluation of the Usefulness of Machine Learning and Artificial Intelligence on Hyperspectral Images in the Diagnosis of Myelodysplastic Syndrome","authors":"Yasuo Ueda, Takafumi Ogawa, Akane Wada, Genshu Tate, Yuta Baba, Tetsuya Fukuda, Toshiko Yamochi","doi":"10.1002/jbio.70319","DOIUrl":"10.1002/jbio.70319","url":null,"abstract":"<p>Hyperspectral imaging (HSI) has gained increasing use in pathological diagnosis in recent years. HSI captures spectral information at wavelengths beyond the visible range. We analyzed specimens from 36 cases diagnosed with myelodysplastic syndrome (MDS) based on bone marrow biopsy or clot specimens at Showa Medical University Fujigaoka Hospital and classified them into three groups based on dysplastic lineage. HSIs of the biopsy specimens were acquired using a pushbroom hyperspectral camera. Spectral data were extracted from annotated target cells and used to train machine learning classifiers. Pixel-level data were divided into training and evaluation sets at the sample level and cross-validated. Our HSI-based artificial intelligence system achieved high accuracy (up to 97%) in bone marrow pathology, outperforming previous RGB-based studies and demonstrating the feasibility of HSI for MDS diagnosis. To the best of our knowledge, this study is the first such evaluation on bone marrow specimens.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70319","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148400258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Characterization of Cerebrovascular Changes in Mice Treated With Alcohol by Photoacoustic Imaging” 修正“用光声成像表征酒精治疗小鼠的脑血管变化”。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-07-02 DOI: 10.1002/jbio.70310

引用次数: 0

Review of the SWIR Windows to Study Osteoarthritis SWIR窗口研究骨关节炎的综述

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-06-30 DOI: 10.1002/jbio.70312

Laura A. Sordillo, Peter P. Sordillo

引用次数: 0

Defining Safe Light Intensity Limits of Near-Infrared Illumination Avoiding Skin Heating in Medical Optical Diagnostic Methods 确定医学光学诊断方法中避免皮肤发热的近红外照明的安全光强极限

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-06-30 DOI: 10.1002/jbio.70320

Anna-Lena Sahlberg, Sophie Helene von der Sahle, Sara Bergsten, Henrik Palme, Emilie Krite Svanberg

{"title":"Defining Safe Light Intensity Limits of Near-Infrared Illumination Avoiding Skin Heating in Medical Optical Diagnostic Methods","authors":"Anna-Lena Sahlberg, Sophie Helene von der Sahle, Sara Bergsten, Henrik Palme, Emilie Krite Svanberg","doi":"10.1002/jbio.70320","DOIUrl":"https://doi.org/10.1002/jbio.70320","url":null,"abstract":"<p>This study examined the thermal response of human skin in vivo to near-infrared illumination (764 nm) delivered either as a free laser beam or through direct contact using a dermal diffusive probe. The aim was to quantify skin heating under increasing illumination intensities and to identify safe exposure thresholds for different skin tones and illumination methods. Research participants with skin tones 1–5 (ranging from light to brown) were included. The results show that skin tone significantly influenced heating under free-beam illumination, whereas temperature increases with dermal probe illumination showed no significant dependence on skin tone. The maximum safe irradiance was determined to be 7.6 mW/mm<sup>2</sup> for free-beam illumination and 2.3 mW/mm<sup>2</sup> for dermal probe illumination, corresponding to a maximum skin temperature of 43°C. These limits apply to skin tones 1–5, and further research is required to establish safety thresholds for skin tone 6.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70320","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reply to “On the Misinterpretation of Fluorescence-Based Quantification in Theranostic Corneal Cross-Linking” by Caruso and Barbaro 回复Caruso和Barbaro的“关于治疗性角膜交联中荧光定量的误解”。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-06-14 DOI: 10.1002/jbio.70292

Giuseppe Lombardo, Marco Lombardo

引用次数: 0

Evaluating Coronavirus Stability: Insights From Raman Spectroscopy and Multivariate Analysis 评估冠状病毒稳定性：来自拉曼光谱和多变量分析的见解

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-06-12 DOI: 10.1002/jbio.70305

Ali Haneen Issmer, Carolina Guerrero-Amelin, Pedro Antonio Mateos-Gomez, Gemma Montalvo, Fernando E. Ortega-Ojeda, Pablo Navarro Caceres, Carmen García-Ruiz

{"title":"Evaluating Coronavirus Stability: Insights From Raman Spectroscopy and Multivariate Analysis","authors":"Ali Haneen Issmer, Carolina Guerrero-Amelin, Pedro Antonio Mateos-Gomez, Gemma Montalvo, Fernando E. Ortega-Ojeda, Pablo Navarro Caceres, Carmen García-Ruiz","doi":"10.1002/jbio.70305","DOIUrl":"https://doi.org/10.1002/jbio.70305","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to evaluate viral stability by probing molecular and structural changes in human coronavirus HCoV-229E under different conditions using Raman spectroscopy coupled with multivariate analysis. Wild-type and GFP-tagged HCoV-229E samples were stored at −20°C for 24, 168, and 504 h, and subjected to thermal treatments at 37°C for 120 min and 95°C for 10 min. Multivariate analyses, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), enabled clear discrimination between virus types, concentrations, and environmental conditions. The observed spectral variations reflect underlying molecular alterations associated with environmental stress. The OPLS-DA models demonstrated strong performance, explaining up to 97% of the variance (<i>R</i><sup>2</sup><i>Y</i>) with a predictive capacity of up to 95% (<i>Q</i><sup>2</sup>). These findings highlight the potential of Raman spectroscopy combined with multivariate analysis as a rapid and non-destructive tool for investigating stability-related molecular changes in coronaviruses under varying conditions.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0