Journal of Biophotonics最新文献_第6页

Development of a High-Throughput Microscope for the Analysis of Peripheral Blood Smears for Anemia Screening 高通量显微镜外周血涂片分析贫血筛查的研制。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-04-06 DOI: 10.1002/jbio.70024

A. M. Arunnagiri, M. Sasikala, N. Ramadass, S. Mullai Venthan

{"title":"Development of a High-Throughput Microscope for the Analysis of Peripheral Blood Smears for Anemia Screening","authors":"A. M. Arunnagiri, M. Sasikala, N. Ramadass, S. Mullai Venthan","doi":"10.1002/jbio.70024","DOIUrl":"10.1002/jbio.70024","url":null,"abstract":"<p>The conventional method of screening for anemia requires pathologists to manually examine slides via microscope, a tedious process during health emergencies. This study presents an automated high-throughput optical digital microscope system capable of sequentially scanning and analyzing 10 blood smear slides per batch in under 15 min using a Laplacian-based autofocusing algorithm at 40x magnification. The acquired images are segmented via the YOLOv5 algorithm, and morphological features of red blood cells (RBCs) are classified using a multilayer perceptron (MLP) model. The system achieved 90.6% accuracy, 95% precision, 91% sensitivity, and 94% specificity in classifying anemia subtypes (macrocytic, microcytic, normocytic) and healthy samples. The trained model is integrated into an Android application for real-time geographic mapping of anemic clusters, enabling healthcare workers to prioritize interventions efficiently. This high-throughput approach eliminates the need for immersion oil and manual slide handling, demonstrating significant potential for rapid, scalable anemia screening in resource-limited settings.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the Intensity of Muscle Contraction by Near-Infrared Spectroscopy, a Potential Application for Scaling Muscle Spasm 用近红外光谱技术评估肌肉收缩强度，在肌肉痉挛测量中的潜在应用。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-04-03 DOI: 10.1002/jbio.70020

Mehdi Nourizadeh, Yekta Saremi, Amir Parham Pirhadi Rad, Sepideh Mortezanezhad, Iman Amani Tehrani, Jocelyn Bégin, Maria Juricic, Kishore Mulpuri, Babak Shadgan

{"title":"Evaluating the Intensity of Muscle Contraction by Near-Infrared Spectroscopy, a Potential Application for Scaling Muscle Spasm","authors":"Mehdi Nourizadeh, Yekta Saremi, Amir Parham Pirhadi Rad, Sepideh Mortezanezhad, Iman Amani Tehrani, Jocelyn Bégin, Maria Juricic, Kishore Mulpuri, Babak Shadgan","doi":"10.1002/jbio.70020","DOIUrl":"10.1002/jbio.70020","url":null,"abstract":"<p>Muscle spasticity, common in conditions such as cerebral palsy, spinal cord injury, and multiple sclerosis, is traditionally assessed using the Modified Ashworth Scale, which lacks consistency. This study evaluates near-infrared spectroscopy (NIRS) as a non-invasive tool for measuring muscle contraction intensity. Thirty-seven healthy adults performed isometric contractions at varying intensities (15%, 30%, 45%, and 60% of maximal voluntary contraction), with NIRS sensors monitoring changes in the Tissue Oxygenation Index (TOI) and electromyography (EMG) measuring muscle activity. Results demonstrated a significant negative correlation between contraction intensity and ΔTOI, indicating that higher contraction levels resulted in greater reductions in muscle oxygenation. Additionally, a multinomial logistic regression model confirmed that TOI could reliably predict contraction intensity (<i>p</i> < 0.001). This technique could provide real-time, objective data for spasticity assessment, potentially improving treatment plans.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Classification Model Based on Hyperspectral Imaging for Predicting Response to Tacrolimus in Patients With Primary Membranous Nephropathy 一种基于高光谱成像预测原发性膜性肾病患者他克莫司反应的新分类模型。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-04-03 DOI: 10.1002/jbio.70025

Meijuan Sun, Wenqiang Zhang, Chongxuan Tian, Ruiyang Wang, Wen Liu, Yang Li, Yang Lv, Zunsong Wang

{"title":"A Novel Classification Model Based on Hyperspectral Imaging for Predicting Response to Tacrolimus in Patients With Primary Membranous Nephropathy","authors":"Meijuan Sun, Wenqiang Zhang, Chongxuan Tian, Ruiyang Wang, Wen Liu, Yang Li, Yang Lv, Zunsong Wang","doi":"10.1002/jbio.70025","DOIUrl":"10.1002/jbio.70025","url":null,"abstract":"<div>\u0000 \u0000 <p>At present, the research to predict the efficacy of tacrolimus (TAC) mainly focuses on serological indexes and urine analysis. Because these indicators are affected by many factors, they cannot accurately predict the therapeutic effect of primary membranous nephropathy (PMN) patients. In this study, a novel classification model (RCN) based on hyperspectral imaging combined with one-dimensional convolutional neural networks (1D CNN) and relevance vector machine (RVM) was proposed for predicting patients' response to TAC. Based on the treatment outcomes of corticosteroids combined with TAC, the patients were divided into a remission group and a nonremission group. Through the analysis of hyperspectral data of pathological slices of patients in both the remission group and the nonremission group, the research results show that the model can effectively extract key features from the spectral data and achieve high classification performance, and it can predict the therapeutic effect of TAC in PMN patients.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facial Skin Aging: Effect of Aging in Different Layers of the Skin on Wrinkles and Sagging 面部皮肤老化：不同皮肤层的老化对皱纹和松弛的影响。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-04-03 DOI: 10.1002/jbio.70028

Dangdang Cheng, Qin Zhang, Wanwen Shan, Feifei Wang

{"title":"Facial Skin Aging: Effect of Aging in Different Layers of the Skin on Wrinkles and Sagging","authors":"Dangdang Cheng, Qin Zhang, Wanwen Shan, Feifei Wang","doi":"10.1002/jbio.70028","DOIUrl":"10.1002/jbio.70028","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To investigate the impact of aging in different skin layers on the formation of wrinkles and sagging.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In a single-center clinical study, the skin micromorphology, wrinkles, and sagging of 34 participants aged 42–60 years who use an antiaging product for 56 days are discussed retrospectively and observationally. The variation trend of each parameter and the correlation between micromorphology parameters and wrinkles and sagging are analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Parameters related to epidermal aging show a strong correlation with wrinkles and sagging, whereas parameters related to dermal aging show no significant correlation with wrinkles and sagging; in addition, parameters related to the dermal–epidermal junction (DEJ) show a moderate correlation with wrinkles and sagging.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>For individuals in the rapid and stable aging phases, aging of the epidermis has a greater impact on wrinkles and sagging than aging of the dermis and DEJ.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Multiple Near-Infrared LEDs (700, 850, and 980 nm) CW-PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts 多种近红外 LED（700、850 和 980 纳米）CW-PBM 对 MG63 骨母细胞线粒体呼吸和基因表达的影响。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-04-03 DOI: 10.1002/jbio.70015

Simone Sleep, Deanne H. Hryciw, Laurence J. Walsh, Eliza Ranjit, Nifty Tomy, Praveen R. Arany, Roy George

{"title":"Effects of Multiple Near-Infrared LEDs (700, 850, and 980 nm) CW-PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts","authors":"Simone Sleep, Deanne H. Hryciw, Laurence J. Walsh, Eliza Ranjit, Nifty Tomy, Praveen R. Arany, Roy George","doi":"10.1002/jbio.70015","DOIUrl":"10.1002/jbio.70015","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Aim</h3>\u0000 \u0000 <p>This study evaluated mitochondrial and osteogenic activity in MG-63 pre-osteoblastic cells after photobiomodulation (PBM) using multiple near-infrared LED sources (Nuralyte) emitting wavelengths from 700 to 1100 nm.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>MG-63 cells were irradiated daily for 3, 5, or 7 days with energy densities of 5.3 J/cm<sup>2</sup> (30 s, optimal dose) and 10.6 J/cm<sup>2</sup> (60 s, high dose). Mitochondrial function was assessed using the XF Seahorse analyzer, and gene expression of osteogenic markers was analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Maximal mitochondrial oxygen consumption rate (OCR) significantly decreased at the optimal dose but increased at the high dose (<i>p</i> < 0.001) in 5-day irradiated cultures. Upregulation of osteogenic markers (OCN, OPN, BMP-2, COL-1, RUNX2) occurred after 3–5 consecutive days of irradiation, with greater activation at the optimal dose.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>MG-63 cells respond to PBM using MNI-LEDs (700, 850, 980 nm) by modulating mitochondrial respiration and boosting bone-related gene expression in a dose- and time-dependent manner.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Instant Diagnosis Using Raman Spectroscopy and Generative Adversarial Networks: A Blood-Based Study on Seasonal Flu, COVID-19, and Dengue 基于拉曼光谱和生成对抗网络的即时诊断：季节性流感、COVID-19和登革热的血液研究

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-04-01 DOI: 10.1002/jbio.70017

Rekha Puthenkaleekkal Thankappan, Dhanya Reghu, Dipak Kumbhar, Ashwin Kotnis, Rashmi Choudhary, Jitendra Singh, A. Raj Kumar Patro, Sarman Singh, Dipankar Nandi, Siva Umapathy

{"title":"Instant Diagnosis Using Raman Spectroscopy and Generative Adversarial Networks: A Blood-Based Study on Seasonal Flu, COVID-19, and Dengue","authors":"Rekha Puthenkaleekkal Thankappan, Dhanya Reghu, Dipak Kumbhar, Ashwin Kotnis, Rashmi Choudhary, Jitendra Singh, A. Raj Kumar Patro, Sarman Singh, Dipankar Nandi, Siva Umapathy","doi":"10.1002/jbio.70017","DOIUrl":"10.1002/jbio.70017","url":null,"abstract":"<div>\u0000 \u0000 <p>Rapid detection of infectious diseases like COVID-19, flu, and dengue is crucial for healthcare professionals preparing for contagious outbreaks. Given the constant mutations in viruses and the recurring emergence of threats like Nipah and Zika, there is an urgent demand for a technology capable of distinguishing between infections that share similar symptoms. In this paper, we utilize laser-based Raman scattered signals from a drop of dried blood plasma, combined with generative artificial intelligence, to provide a rapid and precise diagnosis. Our optimized model exhibits exceptional performance, yielding high predictive scores of 96%, 98%, and 100% for flu, COVID-19, and dengue, respectively. The proposed Raman spectroscopic analysis, with a rapid turnaround time, can ensure a near-accurate diagnosis and proper quarantining of highly infectious cases. Furthermore, the potential extension of our method to include other viral diseases offers an alternative to the challenge of developing different diagnostic kits for each disease.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Detection of SARS-CoV-2 in Clinical Samples Combining a Paper-Based Immunoassay With SERS-Based Read out and Machine Learning 将纸质免疫测定与基于 SERS 的读出和机器学习相结合，快速检测临床样本中的 SARS-CoV-2

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-29 DOI: 10.1002/jbio.70018

Daojian Qi, Yan Wu, Wenbo Mo, Jiaxing Wen, Shuang Ni, Jinglin Huang, Wei Le, Yudan He, Jia Li, Minjie Zhou

引用次数: 0

Exploring the Use of Thermography for Monitoring Physiological Responses to Training in Division-I Collegiate Female Soccer Players 探索利用热成像技术监测高校女足甲级运动员训练后的生理反应。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-25 DOI: 10.1002/jbio.70016

Casey J. Metoyer, Jonathon R. Lever, Valerie Smith-Hale, Cloe Klaus, Cara Ocobock, Alan Huebner, Megan McGinty, Jonathan D. Hauenstein, John P. Wagle

{"title":"Exploring the Use of Thermography for Monitoring Physiological Responses to Training in Division-I Collegiate Female Soccer Players","authors":"Casey J. Metoyer, Jonathon R. Lever, Valerie Smith-Hale, Cloe Klaus, Cara Ocobock, Alan Huebner, Megan McGinty, Jonathan D. Hauenstein, John P. Wagle","doi":"10.1002/jbio.70016","DOIUrl":"10.1002/jbio.70016","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigated the use of thermography as a non-invasive tool to monitor physiological responses to mechanical workload in Division-I female soccer players. A total of 29 student-athletes (age: 19.8 ± 1.1 years) participated, with thermographic imaging conducted throughout training across the competitive season. A total of 8106 observations were analyzed, revealing a weak but significant negative correlation between total player load (TPL) and changes in body region temperature (∆<i>t</i>) (βTPL = −0.00047; <i>p</i> < 0.0001). For every one unit increase in TPL, skin temperature decreased by 0.00047°C on average. Similar patterns were observed in high-minute players (βTPL = −0.0046; <i>p</i> < 0.0001) and when excluding goalkeepers (βTPL = −0.00027, <i>p</i> < 0.001). Interestingly, cooler temperatures were linked to higher workloads in field players, contrasting with previous research. These findings suggest thermography may reflect physiological responses to external workload, with position-specific demands requiring tailored metrics for accurate monitoring.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid, Label-Free Detection of Primary Central Nervous System Lymphoma Using Multiphoton Microscopy 多光子显微镜快速、无标记检测原发性中枢神经系统淋巴瘤。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-24 DOI: 10.1002/jbio.70014

Xingfu Wang, Na Fang, Liwen Hu, Zanyi Wu, Lianhuang Li, Guoping Li, Yupeng Chen, Jianxin Chen, Sheng Zhang, Yueshan Piao

引用次数: 0

Toward Informative Representations of Blood-Based Infrared Spectra via Unsupervised Deep Learning 基于血液的红外光谱的无监督深度学习信息表示。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2025-03-24 DOI: 10.1002/jbio.70011

Corinna Wegner, Zita I. Zarandy, Nico Feiler, Lea Gigou, Timo Halenke, Niklas Leopold-Kerschbaumer, Maik Krusche, Weronika Skibicka, Kosmas V. Kepesidis

引用次数: 0