Rapid, Label-Free Detection of Primary Central Nervous System Lymphoma Using Multiphoton Microscopy

IF 2 3区物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of Biophotonics Pub Date : 2025-03-24 DOI:10.1002/jbio.70014

Xingfu Wang, Na Fang, Liwen Hu, Zanyi Wu, Lianhuang Li, Guoping Li, Yupeng Chen, Jianxin Chen, Sheng Zhang, Yueshan Piao

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引用次数: 0

Abstract

Accurate diagnosis is vital for treating primary central nervous system lymphoma (PCNSL) and determining patient prognosis in clinical practice. Currently, histological analysis stands as the gold standard for definitively diagnosing PCNSL, yet it is time-consuming and invasive. This study introduces multiphoton microscopy (MPM), utilizing second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), to detect human PCNSL. Several diagnostic features of PCNSL, such as increased cellularity, angiocentric infiltration pattern, geographic necrosis, perivascular reticulin deposits, and apoptosis niche, are captured. Moreover, with image processing, the extent of necrosis and perivascular reticulin deposits can be automatically assessed. These research findings underscore the capability of MPM for PCNSL identification. With the advancements in multiphoton endoscopes, in vivo detection of PCNSL may be achievable.

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多光子显微镜快速、无标记检测原发性中枢神经系统淋巴瘤。

在临床实践中，准确诊断对于治疗原发性中枢神经系统淋巴瘤（PCNSL）和确定患者预后至关重要。目前，组织学分析是明确诊断 PCNSL 的金标准，但这种方法耗时且具有侵入性。本研究介绍了利用二次谐波发生（SHG）和双光子激发荧光（TPEF）的多光子显微镜（MPM）来检测人类 PCNSL。它捕捉到了 PCNSL 的几个诊断特征，如细胞增多、血管中心浸润模式、地域性坏死、血管周围网织蛋白沉积和凋亡龛。此外，通过图像处理，还能自动评估坏死和血管周围网织红蛋白沉积的程度。这些研究成果凸显了 MPM 在 PCNSL 鉴定方面的能力。随着多光子内窥镜的发展，PCNSL 的活体检测也许可以实现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biophotonics 生物-生化研究方法

CiteScore

5.70

自引率

7.10%

发文量

248

审稿时长

1 months

期刊介绍： The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.