Journal of biophotonics最新文献

High-Throughput and Stain-Free Morphological Analysis of Sperm Using Optofluidic Time-Stretch Imaging Flow Cytometry.

Journal of biophotonics Pub Date : 2025-03-01 DOI: 10.1002/jbio.202400560

Yueyun Weng, Yujie Zou, Yan Jin, Shubin Wei, Feng Zhang, Rubing Li, Liye Mei, Dongyong Yang, Zhimin Deng, Rui Qu, Dongdong Tang, Du Wang, Fuling Zhou, Sheng Liu, Tailang Yin, Cheng Lei

{"title":"High-Throughput and Stain-Free Morphological Analysis of Sperm Using Optofluidic Time-Stretch Imaging Flow Cytometry.","authors":"Yueyun Weng, Yujie Zou, Yan Jin, Shubin Wei, Feng Zhang, Rubing Li, Liye Mei, Dongyong Yang, Zhimin Deng, Rui Qu, Dongdong Tang, Du Wang, Fuling Zhou, Sheng Liu, Tailang Yin, Cheng Lei","doi":"10.1002/jbio.202400560","DOIUrl":"https://doi.org/10.1002/jbio.202400560","url":null,"abstract":"The incidence of male infertility has kept increasing year by year, severely affecting the sustainability of society. Sperm morphological analysis plays an important role in assessing sperm fertility. However, current sperm morphological analysis is usually performed using microscopic images of stained sperm, which requires complex staining operations, making it difficult to achieve a comprehensive and efficient diagnosis. In this work, we propose and experimentally demonstrate sperm morphological analysis with optofluidic time-stretch (OTS) imaging flow cytometry. Specifically, we capture the stain-free sperm images with high-throughput OTS imaging flow cytometry and analyze the morphological features using a U-Net network. The analysis of 40,000 images from 20 clinical semen samples indicates that our method achieves higher accuracy than the smear test and shows high concordance with the results using comprehensive methods of routine semen examination. This work provides a high-throughput, stain-free sperm morphological analysis method, which holds promise for a comprehensive evaluation of semen.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400560"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accurate Estimation of Methemoglobin and Oxygen Saturation in Skin Tissue Using Diffuse Reflectance Spectroscopy and Artificial Intelligence.

Journal of biophotonics Pub Date : 2025-02-26 DOI: 10.1002/jbio.202400413

Isra Sahli, Wesam Bachir, Moustafa Sayem El-Daher

引用次数: 0

Hyperspectral Imaging of Uterine Fibroids.

Journal of biophotonics Pub Date : 2025-02-25 DOI: 10.1002/jbio.202400499

Aidan M Therien, Jonah A Majumder, Arielle S Joasil, Daniella M Fodera, Kristin M Myers, Xiaowei Chen, Christine P Hendon

{"title":"Hyperspectral Imaging of Uterine Fibroids.","authors":"Aidan M Therien, Jonah A Majumder, Arielle S Joasil, Daniella M Fodera, Kristin M Myers, Xiaowei Chen, Christine P Hendon","doi":"10.1002/jbio.202400499","DOIUrl":"https://doi.org/10.1002/jbio.202400499","url":null,"abstract":"Uterine fibroids are non-cancerous growths of the uterus that affect nearly 70%-80% of women in their lifetimes. Fibroids can cause severe pain, bleeding, and infertility. The main risk of recurrence is smaller fibroids, which are notoriously hard to detect, being missed during a surgical removal procedure, only to enlarge afterwards. In this work, hyperspectral imaging (HSI) datasets were acquired from samples from 10 patients after receiving a hysterectomy. Optical properties including absorption, scattering, and spectral morphology were extracted and fed into machine learning to classify regions as fibroid and myometrium. Top extracted optical features had significant contrast between fibroid and myometrium (p < 0.0001) and were used to train Random Forest (AUC: 0.9985 ± 0.001, Sensitivity: 0.9534 ± 0.019, Specificity: 0.9936 ± 0.009) and Logistic Regression (AUC: 0.9397 ± 0.013, Sensitivity: 0.8405 ± 0.023, Specificity: 0.8895 ± 0.032) with strong performance across testing splits. With HSI, there is contrast between fibroid and myometrium in the human uterus.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400499"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Resolution Oil-Free Imaging of Chromosomes Using Fourier Ptychographic Microscopy. 利用傅立叶平片显微镜对染色体进行高分辨率无油成像。

Journal of biophotonics Pub Date : 2025-02-24 DOI: 10.1002/jbio.202400534

Changliang Yu, Yuguo Tang

引用次数: 0

Scattering Reduction and Axial Resolution Enhancement in Light-Sheet Fluorescence Microscopy.

Journal of biophotonics Pub Date : 2025-02-23 DOI: 10.1002/jbio.202400556

Yang Zhang, Runze Li, Xianghua Yu, Hao Miao, Ruiwen Yang, Xing Li, Junwei Min, Yanlong Yang, Dan Dan, Taiqiang Dai, Liang Kong, Baoli Yao

{"title":"Scattering Reduction and Axial Resolution Enhancement in Light-Sheet Fluorescence Microscopy.","authors":"Yang Zhang, Runze Li, Xianghua Yu, Hao Miao, Ruiwen Yang, Xing Li, Junwei Min, Yanlong Yang, Dan Dan, Taiqiang Dai, Liang Kong, Baoli Yao","doi":"10.1002/jbio.202400556","DOIUrl":"https://doi.org/10.1002/jbio.202400556","url":null,"abstract":"Light-sheet fluorescence microscopy (LSFM) provides an ideal tool for long-term observation of live specimens due to its low photodamage and fast volumetric imaging speed. The wavefront distortions in the illumination path of LSFM will reduce the intensity and broaden the light-sheet thickness, thereby degrading the image quality. We propose to use the wavefront shaping technique to reduce the scattering effect and shrink the light-sheet thickness. Scanning the refocused laser beam to generate LS improves both the fluorescence intensity and the axial resolution. The axial resolution can be further enhanced by subtracting the two images captured via double scanning the samples with the refocused beam and the uncorrected scattered beam for each slice. The axial resolution is improved from 2.2 ± 0.3 to 1.5 ± 0.2 μm across the field of view of 270 μm × 270 μm. The effectiveness of the wavefront shaping subtraction method is demonstrated by imaging fluorescent beads and Aspergillus conidiophores behind a scattering medium.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400556"},"PeriodicalIF":0.0,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectral Differentiation of Esophageal Precancerous Lesion Staging and an Improved Feature Wavelength Selection Method Based on Enhanced Fox Algorithm.

Journal of biophotonics Pub Date : 2025-02-23 DOI: 10.1002/jbio.202400518

Jinbao Zhang, Shuangli Liu, Fanrong Wang, Li Wang, Jiamin Qin, Liming Wen, Weijia Wan

引用次数: 0

External Validation of Raman Spectroscopy for Lyme Disease Diagnostics.

Journal of biophotonics Pub Date : 2025-02-20 DOI: 10.1002/jbio.202400520

Isaac D Juárez, Aidan P Holman, Elizabeth J Horn, Artem S Rogovskyy, Dmitry Kurouski

引用次数: 0

Development and Validation of a Noninvasive Diffuse Reflectance Spectroscopic Method for Bilirubin Estimation in Neonates.

Journal of biophotonics Pub Date : 2025-02-20 DOI: 10.1002/jbio.202400505

Chitra R, Shanthi Prince, Ashok Chandrasekaran, Subash Sundar

引用次数: 0

Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.

Journal of biophotonics Pub Date : 2025-02-19 DOI: 10.1002/jbio.202400450

V I Shcheslavskiy, M V Shirmanova, K S Yashin, A C Rück, M C Skala, W Becker

引用次数: 0

Photobiomodulation of Blue Light Enhance Melanoma Cellular Recovery Following Viral Infection via Mitochondrial Pathways.

Journal of biophotonics Pub Date : 2025-02-17 DOI: 10.1002/jbio.202400525

Haokuan Qin, Hui Jiang, Shijie Huang, Jiali Yang, Qiqi Fu, Ruixiao Zhang, Zeqing Chen, Muqing Liu

{"title":"Photobiomodulation of Blue Light Enhance Melanoma Cellular Recovery Following Viral Infection via Mitochondrial Pathways.","authors":"Haokuan Qin, Hui Jiang, Shijie Huang, Jiali Yang, Qiqi Fu, Ruixiao Zhang, Zeqing Chen, Muqing Liu","doi":"10.1002/jbio.202400525","DOIUrl":"https://doi.org/10.1002/jbio.202400525","url":null,"abstract":"This study aimed to elucidate the response of B16F10 cells to blue light following infection with an empty adenoviral vector (Adv). Twenty-four hours post-infection, the B16F10 cells underwent phototherapy, and a range of cellular parameters were assessed, including cell viability, melanin content, cell cycle progression, reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP), apoptosis, necrosis, lactate dehydrogenase (LDH) release, and autophagy. The findings indicated that Adv infection elevated melanin content and autophagy, resulting in G1 phase cell cycle arrest. In comparison to the control group, light-treated, Adv-infected cells exhibited reduced inhibition rates, LDH release, apoptosis, and necrosis, while MMP and ROS levels were elevated. The study concluded that although Adv, as a gene delivery vector in conjunction with light therapy, significantly modulated melanin content and autophagy. Moreover, while melanin's light absorption capacity mitigated MMP reduction, the resultant excitation molecules generated ROS, enhancing oxidative stress.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400525"},"PeriodicalIF":0.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0