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ElectroFluor Voltage-Sensitive Dyes: Comprehensive Analysis of Wavelength-Dependent Sensitivity and Cross-Channel Bleed-Through.
Journal of biophotonics Pub Date : 2025-03-18 DOI: 10.1002/jbio.70008
Srdjan D Antic, Ping Yan, Corey D Acker, Olivia T Spagnola, Zehra Y Erol, Ozge Baser, Leslie M Loew
{"title":"ElectroFluor Voltage-Sensitive Dyes: Comprehensive Analysis of Wavelength-Dependent Sensitivity and Cross-Channel Bleed-Through.","authors":"Srdjan D Antic, Ping Yan, Corey D Acker, Olivia T Spagnola, Zehra Y Erol, Ozge Baser, Leslie M Loew","doi":"10.1002/jbio.70008","DOIUrl":"https://doi.org/10.1002/jbio.70008","url":null,"abstract":"<p><p>New voltage-sensitive ElectroFluor (EF) dyes that emit across the visible and near-infrared spectrum (e.g., 730 nm) were recently developed. We evaluated EF-530, EF-630, and EF-730p-dyes spectrally orthogonal to green fluorescent protein (GFP)-at excitation wavelengths outside the conventional 470 nm range used for GFP-based indicators. Although previously applied in cardiac voltage imaging, their performance in neuronal tissue remains untested. We performed side-by-side comparisons using population voltage imaging in mouse cerebral cortex slices at optimal excitation wavelengths (530, 630, and 730 nm) and assessed cross-channel signal bleed-through across four excitation wavelengths (475, 530, 630, and 730 nm). All dyes produced robust optical signals at their optimal wavelengths, though non-preferred channels exhibited bleed-through with distinct amplitudes, polarities, and photobleaching patterns. These results provide detailed quantifications of EF dye performance for neuronal population imaging.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70008"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Method for Selecting the Down-Sampling Factor of Photoacoustic Image by Using Cumulative Power Difference in Frequency Domain.
Journal of biophotonics Pub Date : 2025-03-18 DOI: 10.1002/jbio.70013
Shihao Tang, Min Wan, Yameng Zhang, Jiani Li, Ling Tao, Weitao Li
{"title":"Method for Selecting the Down-Sampling Factor of Photoacoustic Image by Using Cumulative Power Difference in Frequency Domain.","authors":"Shihao Tang, Min Wan, Yameng Zhang, Jiani Li, Ling Tao, Weitao Li","doi":"10.1002/jbio.70013","DOIUrl":"https://doi.org/10.1002/jbio.70013","url":null,"abstract":"<p><p>As a novel non-invasive imaging technology, a constraint of photoacoustic microscopy (PAM) is its imaging speed. Often, PAM utilizes sparse spatial sampling, which necessitates extensive prior experimentation to accurately select the down-sampling factors. To overcome this limitation, this study proposes a frequency-domain evaluation index, cumulative power difference (CPD), for rapid selection of the optimal down-sampling factor. We apply the proposed CPD to photoacoustic images of the ear and brain of the mouse. The result shows that as the down-sampling factor increases, there is a similar decreasing trend in the quality of the 20 images. CPD was significantly correlated with PCC/MSE/SSIM (p < 0.001). The findings suggest that CPD, with its ability to evaluate the quality of photoacoustic images and quickly quantify the quality loss of down-sampled images without prior inspection. This study contributes to expanding the application range of PAM and supporting its clinical prospects.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70013"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Combination of Active-Targeted Photodynamic Therapy and Photoactivated Chemotherapy for Enhanced Cancer Treatment. 将主动靶向光动力疗法和光激活化疗相结合，加强癌症治疗。
Journal of biophotonics Pub Date : 2025-03-14 DOI: 10.1002/jbio.70005
Nkune Williams Nkune, Heidi Abrahamse
{"title":"The Combination of Active-Targeted Photodynamic Therapy and Photoactivated Chemotherapy for Enhanced Cancer Treatment.","authors":"Nkune Williams Nkune, Heidi Abrahamse","doi":"10.1002/jbio.70005","DOIUrl":"https://doi.org/10.1002/jbio.70005","url":null,"abstract":"<p><p>Scientists have been actively investigating novel therapies that can effectively eradicate cancer cells with negligible side effects in normal tissues when used alone or in a combinatorial approach. Photodynamic therapy has emerged as a promising non-invasive therapy that integrates photosensitizer, oxygen, and a specific wavelength of light for the treatment of cancer. Despite encouraging outcomes yielded by PDT, conventional PSs are faced with longstanding challenges such as poor water solubility, a short half-life, and off-target toxicity. Development of nanotherapeutics has shown great potential in overcoming this issue. The tumor microenvironment is inherently hypoxic, and this promotes tumor resistance to PDT, as it is oxygen-dependent. Photoactivated chemotherapy, an oxygen-independent light-based therapy, utilizes chemotherapeutic regimens that remain inert until exposed to light, allowing target-specific activation while minimizing off-target toxicity. Integration of these techniques can improve selectivity and yield synergistic cytotoxic effects that could improve cancer treatment.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70005"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnosis and Post-Treatment Follow-Up Evaluation of Melasma Using Optical Coherence Tomography and Deep Learning. 利用光学相干断层扫描和深度学习对黄褐斑进行诊断和治疗后随访评估。
Journal of biophotonics Pub Date : 2025-03-14 DOI: 10.1002/jbio.70006
Xinyuan Cao, Yifeng Lu, Tingting Zhu, Zhilong Yan, Ke Li, Jianhua Mo
{"title":"Diagnosis and Post-Treatment Follow-Up Evaluation of Melasma Using Optical Coherence Tomography and Deep Learning.","authors":"Xinyuan Cao, Yifeng Lu, Tingting Zhu, Zhilong Yan, Ke Li, Jianhua Mo","doi":"10.1002/jbio.70006","DOIUrl":"https://doi.org/10.1002/jbio.70006","url":null,"abstract":"<p><p>Melasma is a common pigmentary disorder accompanied by tissue changes in composition and structure through the epidermis and dermis. In this study, we propose to employ optical coherence tomography (OCT) combined with deep learning techniques for melasma diagnostics. Specifically, a portable spectral domain OCT system with a handheld probe was developed for clinical skin imaging. Then, a diagnostic model was built based on the VGG16 neural network by adding a spatial attention mechanism. The results show that a good differentiation with an accuracy of 94.2% can be achieved among health datasets from healthy volunteers, and melasma and tissue-around-melasma datasets from melasma patients. Moreover, the same trained model was applied to treatment evaluation, showing a good capability to assess antivascular medicine treatment. Thus, it can be concluded that OCT combined with deep learning techniques has a good potential to aid in clinical diagnosis and treatment evaluation of melasma.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70006"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optomechanical Properties of Swine Skin Tissue Treated With a Nontoxic Optical Clearing Agent.
Journal of biophotonics Pub Date : 2025-03-13 DOI: 10.1002/jbio.70007
Noemi Correa, Minji Suh, Abdul Mohizin, Jinu Sudhakaran, Kee Sung Lee, Jung Kyung Kim
{"title":"Optomechanical Properties of Swine Skin Tissue Treated With a Nontoxic Optical Clearing Agent.","authors":"Noemi Correa, Minji Suh, Abdul Mohizin, Jinu Sudhakaran, Kee Sung Lee, Jung Kyung Kim","doi":"10.1002/jbio.70007","DOIUrl":"https://doi.org/10.1002/jbio.70007","url":null,"abstract":"<p><p>This study evaluated the optomechanical and structural properties of individual macroscopic layers in swine skin tissues treated with a nontoxic optical clearing agent. The clearing agent was prepared by dissolving 2,2'-thiodiethanol in a phosphate-buffered solution and applied for up to 6 days. Prolonged clearing increased both the total and unscattered transmittance. Peaks associated with deoxygenated hemoglobin, oxygenated hemoglobin, and water showed marked reduction with extended clearing times. Histopathological examination revealed no significant structural alterations. A clearing time of approximately 4-5 days is recommended for optimal imaging, as it minimizes changes in mechanical properties. These findings support the use of optical clearing in deep tissue imaging, particularly when preserving macroscopic mechanical properties is essential, despite the inherent limitation of sample opacity.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70007"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantitative Detection of Orthotopic Liver Cancer in Mice Using Indocyanine Green and Dynamic Diffuse Fluorescence Tomography Imaging.
Journal of biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.70003
Zhuanxia He, Limin Zhang, Zishuo Li, Xiujun Gao, Yanqi Zhang, Feng Gao
{"title":"Quantitative Detection of Orthotopic Liver Cancer in Mice Using Indocyanine Green and Dynamic Diffuse Fluorescence Tomography Imaging.","authors":"Zhuanxia He, Limin Zhang, Zishuo Li, Xiujun Gao, Yanqi Zhang, Feng Gao","doi":"10.1002/jbio.70003","DOIUrl":"https://doi.org/10.1002/jbio.70003","url":null,"abstract":"<p><p>Orthotopic tumor model has become an essential tool for studying drug biodistribution and tumor progression over time owing to the rapid development of in vivo imaging and immunological science. Dynamic diffuse fluorescence tomography (DFT) is a promising imaging modality that can map the three-dimensional distribution of a fluorophore within the object and capture the metabolic parameters of fluorophores in vivo. It has been widely applied in tumor detection, drug development, and efficacy evaluation. To detect orthotopic liver tumors, we combined indocyanine green (ICG) and a DFT system to perform fluorescence imaging and quantitative analysis for orthotopic liver tumors in mice. The orthotopic liver models were first established, and the liver fluorescence yields were detected in pre- and post-cancerous liver using the DFT system. The results showed that there was higher liver uptake and prolonged retention in orthotopic tumor liver compared to normal liver. Furthermore, the pharmacokinetic parameters suggested the uptake coefficient of tumor liver was above twice that of normal liver, while the excretion rates were similar. Additionally, to intuitively assess tumor occurrence, we propose using normalized fluorescence ratios of 5 and maximum fluorescence values of 0.01 mm<sup>-1</sup> as evaluation criteria. The study demonstrates DFT imaging is a promising tool in orthotopic tumor model detection and drug or agent metabolic evaluation.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70003"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Compact Monocular Video-Ophthalmoscope to Measure Retinal Reflectance Changes to Flicker Light Stimuli.
Journal of biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.202400494
Radim Kolar, Tomas Vicar, Jiri Chmelik, Roman Jakubicek, Jan Odstrcilik, Michal Nohel, Karolina Skorkovska, Ralf P Tornow
{"title":"Compact Monocular Video-Ophthalmoscope to Measure Retinal Reflectance Changes to Flicker Light Stimuli.","authors":"Radim Kolar, Tomas Vicar, Jiri Chmelik, Roman Jakubicek, Jan Odstrcilik, Michal Nohel, Karolina Skorkovska, Ralf P Tornow","doi":"10.1002/jbio.202400494","DOIUrl":"https://doi.org/10.1002/jbio.202400494","url":null,"abstract":"<p><p>This paper describes a compact video-ophthalmoscope (VO) designed for capturing retinal video sequences of the optic nerve head (ONH) under flicker light stimulation. The device uses an OLED display and a fiber optic-coupled LED light source, enabling high-frame-rate video at low illumination intensity (12 μW/cm<sup>2</sup>). Retinal responses were recorded in 10 healthy subjects during flicker light exposure with a pupil irradiance of 2 μW/cm<sup>2</sup>. Following 20 s of stimulation, all subjects displayed changes in retinal reflectance and pulsation attenuation, linked to blood flow and volume variations. These findings suggest that increased blood volume leads to decreased retinal reflectance. Temporal analysis confirmed the ability to capture flicker-induced retinal reflectance changes, indicating its potential for spatial and temporal analysis. Overall, this device offers a portable approach for investigating dynamic retinal responses to light stimuli, which can aid the diagnosis of retinal diseases like diabetic retinopathy, glaucoma, or neurodegenerative diseases affecting retinal blood circulation.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400494"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensing Dog Brain Reactions to Smell by AI Speckle Pattern Analysis.
Journal of biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.202400501
Ilana Propp, Marianna Beiderman, Sergey Agdarov, Yafim Beiderman, Maxi Blum, Zeev Zalevsky
{"title":"Sensing Dog Brain Reactions to Smell by AI Speckle Pattern Analysis.","authors":"Ilana Propp, Marianna Beiderman, Sergey Agdarov, Yafim Beiderman, Maxi Blum, Zeev Zalevsky","doi":"10.1002/jbio.202400501","DOIUrl":"https://doi.org/10.1002/jbio.202400501","url":null,"abstract":"<p><p>Investigation of a dog's brain activity related to their outstanding olfactory capabilities has been a topic of interest among researchers. For this specific study, we identified three areas of the brain that have been shown in previous studies to be relevant during the process of smell discrimination in dogs: the olfactory bulb, hippocampus, and amygdala. We set up a detection structure system based on laser and a camera to capture speckle patterns on the three regions in four dog breeds for smell stimuli: garlic, menthol, alcohol, and marijuana. The results were analyzed using an XGBoost model. Our analysis revealed that the amygdala plays a crucial role in scent differentiation. Our work offers insight into leveraging the features that characterize distinct scents in the canine brain, paving the way for developing a compact device that can interpret and translate a dog's olfactory perceptions for human understanding.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400501"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Noncontact Detection of Blood Coagulation Dynamics Based on Speckle Deviation Analysis Using Optical Coherence Tomography.
Journal of biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.70002
Yun Tang, Zongqing Ma, Yuxian Zhang, Fan Fan, Fan Zhang, Jiang Zhu
{"title":"Noncontact Detection of Blood Coagulation Dynamics Based on Speckle Deviation Analysis Using Optical Coherence Tomography.","authors":"Yun Tang, Zongqing Ma, Yuxian Zhang, Fan Fan, Fan Zhang, Jiang Zhu","doi":"10.1002/jbio.70002","DOIUrl":"https://doi.org/10.1002/jbio.70002","url":null,"abstract":"<p><p>Red blood cells aggregate from individual cells into larger aggregates during blood coagulation. This process causes the light-scattering particles to enlarge and their motion to become restricted. The size and motion of these light-scattering particles during coagulation provide valuable information on the progress of blood coagulation. This paper proposes an optical coherence tomography speckle deviation (OCT-SD) method for the noncontact assessment of blood coagulation dynamics by detecting changes in the size and motion of scattering particles. The coagulation characteristics of static and flowing blood were quantitatively evaluated through coagulation reaction time and clot formation duration by monitoring the blood coagulation process. The OCT imaging, incorporating SD analysis, enables noncontact coagulation assessment. Experimental results demonstrate that the proposed method can efficiently and accurately assess coagulation dynamics, offering significant potential for in situ coagulation detection.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70002"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Confocal Laser Scanning Platform Combined With In Situ High-Resolution Quantitative Phase Imaging.
Journal of biophotonics Pub Date : 2025-03-07 DOI: 10.1002/jbio.202500010
Wenjing Feng, Hongfei Suo, Ying Ma, Hanwei Cheng, Peng Gao, Yunze Lei, Sha An, Juanjuan Zheng, Min Liu
{"title":"Confocal Laser Scanning Platform Combined With In Situ High-Resolution Quantitative Phase Imaging.","authors":"Wenjing Feng, Hongfei Suo, Ying Ma, Hanwei Cheng, Peng Gao, Yunze Lei, Sha An, Juanjuan Zheng, Min Liu","doi":"10.1002/jbio.202500010","DOIUrl":"https://doi.org/10.1002/jbio.202500010","url":null,"abstract":"<p><p>This paper introduces an advanced dual-modality imaging platform by integrating a confocal laser scanning/imaging module with flat-fielding quantitative phase contrast microscopy (FF-QPCM). The platform enables phase/fluorescence dual-modality imaging of the same samples with high resolution and contrast. Using this platform, we observed that confocal fluorescence excitation causes minimal damage to cells, whereas wide-field fluorescence excitation (WFE) results in significant damage, particularly mitochondrial fragmentation and notable alterations in phase values. Additionally, in situ quantitative phase imaging was conducted on live COS7 cells with a specific region irradiated by a confocal laser. The results demonstrated that 5-min continuous confocal laser irradiation affects only COS7 cells with fluorescently labeled mitochondria, causing mitochondrial dysfunction throughout the cell. These findings suggest that integrating FF-QPCM with a confocal laser scanning/imaging module offers a highly efficient, sensitive, and information-rich tool for cell biology research, with broad application potential.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500010"},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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