Casey J Metoyer, Jonathon R Lever, Valerie Smith-Hale, Cloe Klaus, Cara Ocobock, Alan Huebner, Megan McGinty, Jonathan D Hauenstein, John P Wagle
{"title":"Exploring the Use of Thermography for Monitoring Physiological Responses to Training in Division-I Collegiate Female Soccer Players.","authors":"Casey J Metoyer, Jonathon R Lever, Valerie Smith-Hale, Cloe Klaus, Cara Ocobock, Alan Huebner, Megan McGinty, Jonathan D Hauenstein, John P Wagle","doi":"10.1002/jbio.70016","DOIUrl":"https://doi.org/10.1002/jbio.70016","url":null,"abstract":"<p><p>This study investigated the use of thermography as a non-invasive tool to monitor physiological responses to mechanical workload in Division-I female soccer players. A total of 29 student-athletes (age: 19.8 ± 1.1 years) participated, with thermographic imaging conducted throughout training across the competitive season. A total of 8106 observations were analyzed, revealing a weak but significant negative correlation between total player load (TPL) and changes in body region temperature (∆t) (βTPL = -0.00047; p < 0.0001). For every one unit increase in TPL, skin temperature decreased by 0.00047°C on average. Similar patterns were observed in high-minute players (βTPL = -0.0046; p < 0.0001) and when excluding goalkeepers (βTPL = -0.00027, p < 0.001). Interestingly, cooler temperatures were linked to higher workloads in field players, contrasting with previous research. These findings suggest thermography may reflect physiological responses to external workload, with position-specific demands requiring tailored metrics for accurate monitoring.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70016"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rapid, Label-Free Detection of Primary Central Nervous System Lymphoma Using Multiphoton Microscopy.","authors":"Xingfu Wang, Na Fang, Liwen Hu, Zanyi Wu, Lianhuang Li, Guoping Li, Yupeng Chen, Jianxin Chen, Sheng Zhang, Yueshan Piao","doi":"10.1002/jbio.70014","DOIUrl":"https://doi.org/10.1002/jbio.70014","url":null,"abstract":"<p><p>Accurate diagnosis is vital for treating primary central nervous system lymphoma (PCNSL) and determining patient prognosis in clinical practice. Currently, histological analysis stands as the gold standard for definitively diagnosing PCNSL, yet it is time-consuming and invasive. This study introduces multiphoton microscopy (MPM), utilizing second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), to detect human PCNSL. Several diagnostic features of PCNSL, such as increased cellularity, angiocentric infiltration pattern, geographic necrosis, perivascular reticulin deposits, and apoptosis niche, are captured. Moreover, with image processing, the extent of necrosis and perivascular reticulin deposits can be automatically assessed. These research findings underscore the capability of MPM for PCNSL identification. With the advancements in multiphoton endoscopes, in vivo detection of PCNSL may be achievable.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70014"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Corinna Wegner, Zita I Zarandy, Nico Feiler, Lea Gigou, Timo Halenke, Niklas Leopold-Kerschbaumer, Maik Krusche, Weronika Skibicka, Kosmas V Kepesidis
{"title":"Toward Informative Representations of Blood-Based Infrared Spectra via Unsupervised Deep Learning.","authors":"Corinna Wegner, Zita I Zarandy, Nico Feiler, Lea Gigou, Timo Halenke, Niklas Leopold-Kerschbaumer, Maik Krusche, Weronika Skibicka, Kosmas V Kepesidis","doi":"10.1002/jbio.70011","DOIUrl":"https://doi.org/10.1002/jbio.70011","url":null,"abstract":"<p><p>This study explores using unsupervised deep learning to find a low-dimensional representation of infrared molecular fingerprints of human blood. We developed a fully convolutional denoising autoencoder to process Fourier transform infrared (FTIR) spectroscopy data, aiming to condense the spectra into a set of latent variables. By utilizing the autoencoder's bottleneck architecture and a custom loss function, we effectively reduced noise while retaining essential molecular information. This method improved lung cancer detection accuracy by 2.6 percentage points in a case-control study. The resulting latent space not only compacts spectral data, but also highlights variables linked to disease presence, offering potential for improving diagnostics. Trial Registration: German Clinical Trials Register (DRKS): DRKS00013217.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70011"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huihui Liu, Sunil Kumar, Edwin Garcia, Darren Ennis, Iain A McNeish, Paul M W French
{"title":"PolSpec: Polarisation-Based Detection for Versatile, Cost-Effective Rapid Hyperspectral Imaging.","authors":"Huihui Liu, Sunil Kumar, Edwin Garcia, Darren Ennis, Iain A McNeish, Paul M W French","doi":"10.1002/jbio.70012","DOIUrl":"https://doi.org/10.1002/jbio.70012","url":null,"abstract":"<p><p>\"PolSpec\" is a flexible, cost-effective approach for rapid (including single-shot) spectrally resolved imaging. While established approaches, e.g., using cascades of dichroic beamsplitters, diffractive image splitters, or mosaic filters, typically have pre-determined spectral detection bands with cost and experimental complexity scaling with the number of spectral channels, PolSpec uses polarisation optics to provide continuously varying transmission across a configurable spectral range to generate \"spectral modulation vectors\" that can represent specific spectral signatures with lower data volumes than full spectral profiles. It can be implemented with almost any detector. Here we demonstrate low-cost single-shot widefield PolSpec-based hyperspectral imaging using a polarisation-resolving camera.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70012"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aline Knab, Caroline Giardina, Shane T Grey, Ewa M Goldys, Jared M Campbell
{"title":"Illuminating Immunity: A Systematic Review of Immune Cell Autofluorescence.","authors":"Aline Knab, Caroline Giardina, Shane T Grey, Ewa M Goldys, Jared M Campbell","doi":"10.1002/jbio.202400576","DOIUrl":"https://doi.org/10.1002/jbio.202400576","url":null,"abstract":"<p><p>Immunophenotyping provides valuable prognostic and diagnostic information, but is technically complex and expensive. The assessment of autofluorescence is label-free and provides complex information on cell identity. However, research on its application to immunophenotyping has been heterogenous. This systematic review was carried out to identify and synthesise all available evidence on the use of autofluorescence for immunophenotyping. Eighty three full texts were included. There was a focus on neutrophils (20 papers) and macrophages (22 papers) with alveolar macrophages (13 papers) forming a subcategory. Seven studies investigated monocytes, three focused on microglia, two on dendritic cells, five on mast cells, nine on granulocytes, thirteen on eosinophils, one on erythrophagocytic cells, and one on natural killer cells. Eleven studies investigated uncategorised immune cell populations. Translation of findings into clinical immunophenotyping requires the application of reproducible methods, along with clear reporting of excitation and emission parameters, and a greater focus on clinical and primary samples.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400576"},"PeriodicalIF":0.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W Anto Win Shalini, T Rajalakshmi, S Vasanthadev Suryakala
{"title":"Enhancing the Diagnostic Evaluation of Thyroid Functionality Using Diffuse Reflectance Spectroscopy and Regression Models.","authors":"W Anto Win Shalini, T Rajalakshmi, S Vasanthadev Suryakala","doi":"10.1002/jbio.70010","DOIUrl":"https://doi.org/10.1002/jbio.70010","url":null,"abstract":"<p><p>Thyroid dysfunction is a prevalent global health concern that necessitates the development of effective and non-invasive screening methods to enable early detection. The application of Diffuse Reflectance Spectroscopy (DRS) in conjunction with preprocessing and predictive models for thyroid dysfunction diagnosis is investigated. The raw spectral data captured from 31 individuals with thyroid dysfunction are subjected to spectral preprocessing techniques like, Standard Normal Variate (SNV), Multiplicative Scatter Correction (MSC), and Baseline Correction. The preprocessed data subjected to regression models like Partial Least Squares Regression (PLSR), Principal Component Regression (PCR), LASSO, Random Forest, Ridge Regression, Gaussian Process Regression (GPR), and Bayesian Regression were employed to analyse the efficacy of the models. The PLSR model in concurrence with SNV outperforms other regression models by achieving an R<sup>2</sup> of 0.93, RMSE of 0.29, and MSE of 0.08, indicating low predictive error. The goodness of fit was also evaluated using Pearson's chi-squared test.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70010"},"PeriodicalIF":0.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ni He, Chenrui Li, Jiali Liu, Xiaolong Zhang, Liming Li, Cuixia Dai
{"title":"Variation of Structures and Blood Vessels of Visual Nervous System With Age.","authors":"Ni He, Chenrui Li, Jiali Liu, Xiaolong Zhang, Liming Li, Cuixia Dai","doi":"10.1002/jbio.70009","DOIUrl":"https://doi.org/10.1002/jbio.70009","url":null,"abstract":"<p><p>Aging is a process of progressive functional decline associated with increasing age. The process and mechanism of aging have long been widely concerned, but long-term in vivo evaluations of the visual nervous system have not been previously reported. In this study, naturally aging mouse models were used for long-term serial evaluation, and the changes in structure and blood flow of the retina and cerebral cortex were systematically analyzed. Optical Coherence Tomography (OCT) and Optical Coherence Tomography Angiography (OCTA) were performed on mice at 3, 6, 9, and 12 months of age, respectively. Structural and vascular changes with age were quantitatively evaluated. Results show that the reduction of structural thickness and vascular density of the retinal and cerebral cortex is observed. A significant correlation is also found between structural and vascular changes in the retina and cerebral cortex, indicating a consistent impact of aging on the visual nervous system.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70009"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shihao Tang, Min Wan, Yameng Zhang, Jiani Li, Ling Tao, Weitao Li
{"title":"Method for Selecting the Down-Sampling Factor of Photoacoustic Image by Using Cumulative Power Difference in Frequency Domain.","authors":"Shihao Tang, Min Wan, Yameng Zhang, Jiani Li, Ling Tao, Weitao Li","doi":"10.1002/jbio.70013","DOIUrl":"https://doi.org/10.1002/jbio.70013","url":null,"abstract":"<p><p>As a novel non-invasive imaging technology, a constraint of photoacoustic microscopy (PAM) is its imaging speed. Often, PAM utilizes sparse spatial sampling, which necessitates extensive prior experimentation to accurately select the down-sampling factors. To overcome this limitation, this study proposes a frequency-domain evaluation index, cumulative power difference (CPD), for rapid selection of the optimal down-sampling factor. We apply the proposed CPD to photoacoustic images of the ear and brain of the mouse. The result shows that as the down-sampling factor increases, there is a similar decreasing trend in the quality of the 20 images. CPD was significantly correlated with PCC/MSE/SSIM (p < 0.001). The findings suggest that CPD, with its ability to evaluate the quality of photoacoustic images and quickly quantify the quality loss of down-sampled images without prior inspection. This study contributes to expanding the application range of PAM and supporting its clinical prospects.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70013"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Srdjan D Antic, Ping Yan, Corey D Acker, Olivia T Spagnola, Zehra Y Erol, Ozge Baser, Leslie M Loew
{"title":"ElectroFluor Voltage-Sensitive Dyes: Comprehensive Analysis of Wavelength-Dependent Sensitivity and Cross-Channel Bleed-Through.","authors":"Srdjan D Antic, Ping Yan, Corey D Acker, Olivia T Spagnola, Zehra Y Erol, Ozge Baser, Leslie M Loew","doi":"10.1002/jbio.70008","DOIUrl":"https://doi.org/10.1002/jbio.70008","url":null,"abstract":"<p><p>New voltage-sensitive ElectroFluor (EF) dyes that emit across the visible and near-infrared spectrum (e.g., 730 nm) were recently developed. We evaluated EF-530, EF-630, and EF-730p-dyes spectrally orthogonal to green fluorescent protein (GFP)-at excitation wavelengths outside the conventional 470 nm range used for GFP-based indicators. Although previously applied in cardiac voltage imaging, their performance in neuronal tissue remains untested. We performed side-by-side comparisons using population voltage imaging in mouse cerebral cortex slices at optimal excitation wavelengths (530, 630, and 730 nm) and assessed cross-channel signal bleed-through across four excitation wavelengths (475, 530, 630, and 730 nm). All dyes produced robust optical signals at their optimal wavelengths, though non-preferred channels exhibited bleed-through with distinct amplitudes, polarities, and photobleaching patterns. These results provide detailed quantifications of EF dye performance for neuronal population imaging.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70008"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}