Journal of biophotonics最新文献_第3页

Differentiation of Bacterial Species in Liquid Culture Using Laser Speckle Contrast Imaging.

Journal of biophotonics Pub Date : 2025-03-05 DOI: 10.1002/jbio.202400565

Priya Krishnamurthy, Roshni Shetty, Kiran Philip Isaac, Sujatha Narayanan Unni, Sudhanarayani S Rao, Krupakar Parthasarathy

引用次数: 0

Label-Free Multiphoton Microscopy for Diagnosis of Psoriasis.

Journal of biophotonics Pub Date : 2025-03-05 DOI: 10.1002/jbio.202500025

Liqin Zheng, Liuzhi Wu, Wenwen Zhong, Shengrong Du, Zhen Li, Xi Chen, Jianping Huang, Shulian Wu, Lina Liu, Jianxin Chen, Yelin Wu

引用次数: 0

Visible Blue Light Does Not Induce DNA Damage in Human Dermal Fibroblasts.

Journal of biophotonics Pub Date : 2025-03-04 DOI: 10.1002/jbio.202400510

Margaret Kabakova, Jennifer Wang, Julia Stolyar, Evan Austin, Jared Jagdeo

引用次数: 0

Effect of Sequential Repetitive Transcranial Magnetic Stimulation With Bilateral Arm Training on the Brain Effective Connectivity in Chronic Stroke.

Journal of biophotonics Pub Date : 2025-03-04 DOI: 10.1002/jbio.202400508

Wenhao Li, Ping Cao, Ran Wei, Duo Wai-Chi Wong

{"title":"Effect of Sequential Repetitive Transcranial Magnetic Stimulation With Bilateral Arm Training on the Brain Effective Connectivity in Chronic Stroke.","authors":"Wenhao Li, Ping Cao, Ran Wei, Duo Wai-Chi Wong","doi":"10.1002/jbio.202400508","DOIUrl":"https://doi.org/10.1002/jbio.202400508","url":null,"abstract":"This study investigated the effects of combining repetitive transcranial magnetic stimulation (rTMS) with bilateral arm training (BAT) on effective brain connectivity in chronic stroke patients using functional near-infrared spectroscopy. Fifteen post-stroke patients and fifteen healthy individuals were enrolled. Coupling function analysis was performed to evaluate the effective connectivity inflow, outflow, and the dominant information flow (DIF) during standalone BAT and combined rTMS-BAT therapy. Significant task-related alterations were observed in the ipsilesional supplementary motor area and occipital lobe (OL) of stroke patients undergoing rTMS-BAT. During BAT, stroke patients exhibited more pronounced DIF from the OL to motor areas compared to healthy controls. Furthermore, in the rTMS-BAT condition, patients demonstrated enhanced DIF from the ipsilesional OL and contralesional prefrontal cortex to ipsilesional motor areas. These findings suggested a potential synergistic effect on cortical reorganization through the sequential combination of task-related training and TMS in chronic stroke patients, offering insights into rehabilitation strategies.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400508"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the Impact of Long-Term Alcohol Consumption on Serum Chemical Changes: Fourier Transform Infrared Spectroscopy for Human Blood Serum.

Journal of biophotonics Pub Date : 2025-03-04 DOI: 10.1002/jbio.202400550

Zozan Guleken, Devrim Sarıbal, Hasan Mırsal, Jozef Cebulski, Zeynep Ceylan, Joanna Depciuch

{"title":"Investigating the Impact of Long-Term Alcohol Consumption on Serum Chemical Changes: Fourier Transform Infrared Spectroscopy for Human Blood Serum.","authors":"Zozan Guleken, Devrim Sarıbal, Hasan Mırsal, Jozef Cebulski, Zeynep Ceylan, Joanna Depciuch","doi":"10.1002/jbio.202400550","DOIUrl":"https://doi.org/10.1002/jbio.202400550","url":null,"abstract":"Chronic alcohol consumption significantly impacts physiological and neurological functions. This study aimed to investigate the biochemical alterations in serum associated with long-term alcohol use using Fourier Transform Infrared (FTIR) spectroscopy. Serum samples from control and alcohol use disorder (AUD) were analyzed, and their spectra were compared. Multivariate analysis techniques, including Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA), were employed to differentiate the groups. A machine learning model, Grid Search-Support Vector Machine Discriminant Analysis (GS-SVMDA), was developed to classify samples with high accuracy. Significant differences in the absorbance values of specific functional groups, particularly those associated with phospholipids, amides, and fatty acids revealed. The AUD exhibited lower levels of these biomolecules. The models achieved perfect classification, demonstrating the potential ofFTIR spectroscopy as a non-invasive tool for diagnosing AUD. Findings contribute to a better understanding of the biochemical mechanisms underlying alcohol addiction and may aid in the development of novel diagnostic and therapeutic strategies.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400550"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Throughput and Stain-Free Morphological Analysis of Sperm Using Optofluidic Time-Stretch Imaging Flow Cytometry. 利用光流体时间拉伸成像流式细胞术对精子进行高通量和无染色形态学分析

Journal of biophotonics Pub Date : 2025-03-01 DOI: 10.1002/jbio.202400560

Yueyun Weng, Yujie Zou, Yan Jin, Shubin Wei, Feng Zhang, Rubing Li, Liye Mei, Dongyong Yang, Zhimin Deng, Rui Qu, Dongdong Tang, Du Wang, Fuling Zhou, Sheng Liu, Tailang Yin, Cheng Lei

{"title":"High-Throughput and Stain-Free Morphological Analysis of Sperm Using Optofluidic Time-Stretch Imaging Flow Cytometry.","authors":"Yueyun Weng, Yujie Zou, Yan Jin, Shubin Wei, Feng Zhang, Rubing Li, Liye Mei, Dongyong Yang, Zhimin Deng, Rui Qu, Dongdong Tang, Du Wang, Fuling Zhou, Sheng Liu, Tailang Yin, Cheng Lei","doi":"10.1002/jbio.202400560","DOIUrl":"https://doi.org/10.1002/jbio.202400560","url":null,"abstract":"The incidence of male infertility has kept increasing year by year, severely affecting the sustainability of society. Sperm morphological analysis plays an important role in assessing sperm fertility. However, current sperm morphological analysis is usually performed using microscopic images of stained sperm, which requires complex staining operations, making it difficult to achieve a comprehensive and efficient diagnosis. In this work, we propose and experimentally demonstrate sperm morphological analysis with optofluidic time-stretch (OTS) imaging flow cytometry. Specifically, we capture the stain-free sperm images with high-throughput OTS imaging flow cytometry and analyze the morphological features using a U-Net network. The analysis of 40,000 images from 20 clinical semen samples indicates that our method achieves higher accuracy than the smear test and shows high concordance with the results using comprehensive methods of routine semen examination. This work provides a high-throughput, stain-free sperm morphological analysis method, which holds promise for a comprehensive evaluation of semen.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400560"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accurate Estimation of Methemoglobin and Oxygen Saturation in Skin Tissue Using Diffuse Reflectance Spectroscopy and Artificial Intelligence.

Journal of biophotonics Pub Date : 2025-02-26 DOI: 10.1002/jbio.202400413

Isra Sahli, Wesam Bachir, Moustafa Sayem El-Daher

引用次数: 0

Hyperspectral Imaging of Uterine Fibroids.

Journal of biophotonics Pub Date : 2025-02-25 DOI: 10.1002/jbio.202400499

Aidan M Therien, Jonah A Majumder, Arielle S Joasil, Daniella M Fodera, Kristin M Myers, Xiaowei Chen, Christine P Hendon

{"title":"Hyperspectral Imaging of Uterine Fibroids.","authors":"Aidan M Therien, Jonah A Majumder, Arielle S Joasil, Daniella M Fodera, Kristin M Myers, Xiaowei Chen, Christine P Hendon","doi":"10.1002/jbio.202400499","DOIUrl":"https://doi.org/10.1002/jbio.202400499","url":null,"abstract":"Uterine fibroids are non-cancerous growths of the uterus that affect nearly 70%-80% of women in their lifetimes. Fibroids can cause severe pain, bleeding, and infertility. The main risk of recurrence is smaller fibroids, which are notoriously hard to detect, being missed during a surgical removal procedure, only to enlarge afterwards. In this work, hyperspectral imaging (HSI) datasets were acquired from samples from 10 patients after receiving a hysterectomy. Optical properties including absorption, scattering, and spectral morphology were extracted and fed into machine learning to classify regions as fibroid and myometrium. Top extracted optical features had significant contrast between fibroid and myometrium (p < 0.0001) and were used to train Random Forest (AUC: 0.9985 ± 0.001, Sensitivity: 0.9534 ± 0.019, Specificity: 0.9936 ± 0.009) and Logistic Regression (AUC: 0.9397 ± 0.013, Sensitivity: 0.8405 ± 0.023, Specificity: 0.8895 ± 0.032) with strong performance across testing splits. With HSI, there is contrast between fibroid and myometrium in the human uterus.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400499"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Resolution Oil-Free Imaging of Chromosomes Using Fourier Ptychographic Microscopy. 利用傅立叶平片显微镜对染色体进行高分辨率无油成像。

Journal of biophotonics Pub Date : 2025-02-24 DOI: 10.1002/jbio.202400534

Changliang Yu, Yuguo Tang

引用次数: 0

Scattering Reduction and Axial Resolution Enhancement in Light-Sheet Fluorescence Microscopy.

Journal of biophotonics Pub Date : 2025-02-23 DOI: 10.1002/jbio.202400556

Yang Zhang, Runze Li, Xianghua Yu, Hao Miao, Ruiwen Yang, Xing Li, Junwei Min, Yanlong Yang, Dan Dan, Taiqiang Dai, Liang Kong, Baoli Yao

{"title":"Scattering Reduction and Axial Resolution Enhancement in Light-Sheet Fluorescence Microscopy.","authors":"Yang Zhang, Runze Li, Xianghua Yu, Hao Miao, Ruiwen Yang, Xing Li, Junwei Min, Yanlong Yang, Dan Dan, Taiqiang Dai, Liang Kong, Baoli Yao","doi":"10.1002/jbio.202400556","DOIUrl":"https://doi.org/10.1002/jbio.202400556","url":null,"abstract":"Light-sheet fluorescence microscopy (LSFM) provides an ideal tool for long-term observation of live specimens due to its low photodamage and fast volumetric imaging speed. The wavefront distortions in the illumination path of LSFM will reduce the intensity and broaden the light-sheet thickness, thereby degrading the image quality. We propose to use the wavefront shaping technique to reduce the scattering effect and shrink the light-sheet thickness. Scanning the refocused laser beam to generate LS improves both the fluorescence intensity and the axial resolution. The axial resolution can be further enhanced by subtracting the two images captured via double scanning the samples with the refocused beam and the uncorrected scattered beam for each slice. The axial resolution is improved from 2.2 ± 0.3 to 1.5 ± 0.2 μm across the field of view of 270 μm × 270 μm. The effectiveness of the wavefront shaping subtraction method is demonstrated by imaging fluorescent beads and Aspergillus conidiophores behind a scattering medium.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400556"},"PeriodicalIF":0.0,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0