Journal of biophotonics最新文献_第3页

The Combination of Active-Targeted Photodynamic Therapy and Photoactivated Chemotherapy for Enhanced Cancer Treatment. 将主动靶向光动力疗法和光激活化疗相结合，加强癌症治疗。

Journal of biophotonics Pub Date : 2025-03-14 DOI: 10.1002/jbio.70005

Nkune Williams Nkune, Heidi Abrahamse

引用次数: 0

Diagnosis and Post-Treatment Follow-Up Evaluation of Melasma Using Optical Coherence Tomography and Deep Learning. 利用光学相干断层扫描和深度学习对黄褐斑进行诊断和治疗后随访评估。

Journal of biophotonics Pub Date : 2025-03-14 DOI: 10.1002/jbio.70006

Xinyuan Cao, Yifeng Lu, Tingting Zhu, Zhilong Yan, Ke Li, Jianhua Mo

引用次数: 0

Optomechanical Properties of Swine Skin Tissue Treated With a Nontoxic Optical Clearing Agent. 无毒光清除剂处理猪皮组织的光力学性能。

Journal of biophotonics Pub Date : 2025-03-13 DOI: 10.1002/jbio.70007

Noemi Correa, Minji Suh, Abdul Mohizin, Jinu Sudhakaran, Kee Sung Lee, Jung Kyung Kim

引用次数: 0

Quantitative Detection of Orthotopic Liver Cancer in Mice Using Indocyanine Green and Dynamic Diffuse Fluorescence Tomography Imaging. 吲哚菁绿和动态漫射荧光断层成像定量检测小鼠原位肝癌。

Journal of biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.70003

Zhuanxia He, Limin Zhang, Zishuo Li, Xiujun Gao, Yanqi Zhang, Feng Gao

{"title":"Quantitative Detection of Orthotopic Liver Cancer in Mice Using Indocyanine Green and Dynamic Diffuse Fluorescence Tomography Imaging.","authors":"Zhuanxia He, Limin Zhang, Zishuo Li, Xiujun Gao, Yanqi Zhang, Feng Gao","doi":"10.1002/jbio.70003","DOIUrl":"https://doi.org/10.1002/jbio.70003","url":null,"abstract":"Orthotopic tumor model has become an essential tool for studying drug biodistribution and tumor progression over time owing to the rapid development of in vivo imaging and immunological science. Dynamic diffuse fluorescence tomography (DFT) is a promising imaging modality that can map the three-dimensional distribution of a fluorophore within the object and capture the metabolic parameters of fluorophores in vivo. It has been widely applied in tumor detection, drug development, and efficacy evaluation. To detect orthotopic liver tumors, we combined indocyanine green (ICG) and a DFT system to perform fluorescence imaging and quantitative analysis for orthotopic liver tumors in mice. The orthotopic liver models were first established, and the liver fluorescence yields were detected in pre- and post-cancerous liver using the DFT system. The results showed that there was higher liver uptake and prolonged retention in orthotopic tumor liver compared to normal liver. Furthermore, the pharmacokinetic parameters suggested the uptake coefficient of tumor liver was above twice that of normal liver, while the excretion rates were similar. Additionally, to intuitively assess tumor occurrence, we propose using normalized fluorescence ratios of 5 and maximum fluorescence values of 0.01 mm-1 as evaluation criteria. The study demonstrates DFT imaging is a promising tool in orthotopic tumor model detection and drug or agent metabolic evaluation.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70003"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Compact Monocular Video-Ophthalmoscope to Measure Retinal Reflectance Changes to Flicker Light Stimuli. 紧凑型单眼视频检眼镜测量视网膜对闪烁光刺激的反射变化。

Journal of biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.202400494

Radim Kolar, Tomas Vicar, Jiri Chmelik, Roman Jakubicek, Jan Odstrcilik, Michal Nohel, Karolina Skorkovska, Ralf P Tornow

{"title":"Compact Monocular Video-Ophthalmoscope to Measure Retinal Reflectance Changes to Flicker Light Stimuli.","authors":"Radim Kolar, Tomas Vicar, Jiri Chmelik, Roman Jakubicek, Jan Odstrcilik, Michal Nohel, Karolina Skorkovska, Ralf P Tornow","doi":"10.1002/jbio.202400494","DOIUrl":"https://doi.org/10.1002/jbio.202400494","url":null,"abstract":"This paper describes a compact video-ophthalmoscope (VO) designed for capturing retinal video sequences of the optic nerve head (ONH) under flicker light stimulation. The device uses an OLED display and a fiber optic-coupled LED light source, enabling high-frame-rate video at low illumination intensity (12 μW/cm2). Retinal responses were recorded in 10 healthy subjects during flicker light exposure with a pupil irradiance of 2 μW/cm2. Following 20 s of stimulation, all subjects displayed changes in retinal reflectance and pulsation attenuation, linked to blood flow and volume variations. These findings suggest that increased blood volume leads to decreased retinal reflectance. Temporal analysis confirmed the ability to capture flicker-induced retinal reflectance changes, indicating its potential for spatial and temporal analysis. Overall, this device offers a portable approach for investigating dynamic retinal responses to light stimuli, which can aid the diagnosis of retinal diseases like diabetic retinopathy, glaucoma, or neurodegenerative diseases affecting retinal blood circulation.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400494"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensing Dog Brain Reactions to Smell by AI Speckle Pattern Analysis. 通过人工智能斑点模式分析感知狗的大脑对气味的反应。

Journal of biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.202400501

Ilana Propp, Marianna Beiderman, Sergey Agdarov, Yafim Beiderman, Maxi Blum, Zeev Zalevsky

引用次数: 0

Noncontact Detection of Blood Coagulation Dynamics Based on Speckle Deviation Analysis Using Optical Coherence Tomography. 基于光学相干断层成像散斑偏差分析的非接触血液凝固动力学检测。

Journal of biophotonics Pub Date : 2025-03-11 DOI: 10.1002/jbio.70002

Yun Tang, Zongqing Ma, Yuxian Zhang, Fan Fan, Fan Zhang, Jiang Zhu

引用次数: 0

Confocal Laser Scanning Platform Combined With In Situ High-Resolution Quantitative Phase Imaging. 结合原位高分辨率定量相位成像的共聚焦激光扫描平台。

Journal of biophotonics Pub Date : 2025-03-07 DOI: 10.1002/jbio.202500010

Wenjing Feng, Hongfei Suo, Ying Ma, Hanwei Cheng, Peng Gao, Yunze Lei, Sha An, Juanjuan Zheng, Min Liu

{"title":"Confocal Laser Scanning Platform Combined With In Situ High-Resolution Quantitative Phase Imaging.","authors":"Wenjing Feng, Hongfei Suo, Ying Ma, Hanwei Cheng, Peng Gao, Yunze Lei, Sha An, Juanjuan Zheng, Min Liu","doi":"10.1002/jbio.202500010","DOIUrl":"https://doi.org/10.1002/jbio.202500010","url":null,"abstract":"This paper introduces an advanced dual-modality imaging platform by integrating a confocal laser scanning/imaging module with flat-fielding quantitative phase contrast microscopy (FF-QPCM). The platform enables phase/fluorescence dual-modality imaging of the same samples with high resolution and contrast. Using this platform, we observed that confocal fluorescence excitation causes minimal damage to cells, whereas wide-field fluorescence excitation (WFE) results in significant damage, particularly mitochondrial fragmentation and notable alterations in phase values. Additionally, in situ quantitative phase imaging was conducted on live COS7 cells with a specific region irradiated by a confocal laser. The results demonstrated that 5-min continuous confocal laser irradiation affects only COS7 cells with fluorescently labeled mitochondria, causing mitochondrial dysfunction throughout the cell. These findings suggest that integrating FF-QPCM with a confocal laser scanning/imaging module offers a highly efficient, sensitive, and information-rich tool for cell biology research, with broad application potential.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500010"},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Full-Field Assessment of Geometry and Collagenous Architecture of Porcine Valve Leaflets via Laser Micrometry and Quantitative Polarized Light Imaging. 利用激光显微测量和定量偏振光成像技术对猪瓣膜小叶的几何结构和胶原结构进行全方位评估。

Journal of biophotonics Pub Date : 2025-03-06 DOI: 10.1002/jbio.202400511

Shreya Sreedhar, Daniel P Pearce, Colleen M Witzenburg

{"title":"Full-Field Assessment of Geometry and Collagenous Architecture of Porcine Valve Leaflets via Laser Micrometry and Quantitative Polarized Light Imaging.","authors":"Shreya Sreedhar, Daniel P Pearce, Colleen M Witzenburg","doi":"10.1002/jbio.202400511","DOIUrl":"https://doi.org/10.1002/jbio.202400511","url":null,"abstract":"Despite the frequent failure of aortic valves and pediatric usage of pulmonary valves as a replacement, comparative studies on their full-field collagenous architecture and macroscale geometries are limited. We applied laser micrometry and quantitative polarized light imaging (QPLI), a novel technique for assessing collagen fiber organization, to porcine aortic (n = 8) and pulmonary (n = 8) valve leaflets to non-destructively compare thickness and anisotropy. We confirmed (1) light intensity and sample thickness are inversely related and (2) aortic valve leaflets are thicker with decreased fiber organization when unloaded. To demonstrate the ability of QPLI to capture dynamic collagen fiber alignment, we imaged leaflets during equibiaxial loading. There was an increase in the aortic leaflet's degree of alignment throughout loading, whereas the pulmonary valve leaflet exhibited relatively unchanged alignment. Ultimately, understanding the full-field organization of a leaflet's heterogeneous ECM and how it is altered by pathology can inform therapy development.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400511"},"PeriodicalIF":0.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance. 荧光寿命成像技术的原理、应用及临床意义综述

Journal of biophotonics Pub Date : 2025-02-19 DOI: 10.1002/jbio.202400450

V I Shcheslavskiy, M V Shirmanova, K S Yashin, A C Rück, M C Skala, W Becker

引用次数: 0