Journal of biophotonics最新文献_第3页

Optical Coherence Tomography and Angiography Image Enhancement Using Optical Clearing Agent Tartrazine. 光学相干断层扫描和血管造影图像增强使用光学清除剂酒黄石。

IF 2.3

Journal of biophotonics Pub Date : 2025-09-01 DOI: 10.1002/jbio.202500297

Yilin Liang, Xiaochen Meng, Chongyang Wang, Jiawei Ma, Xuanye Zhang, Fan Fan, Jiang Zhu

引用次数: 0

Photonics Detection of Molecular-Specific Spatial Structural Alterations of Cells due to Chronic Alcoholism and Probiotics Treatments in Colon Cancer. 结肠癌慢性酒精中毒和益生菌治疗引起的细胞分子特异性空间结构改变的光子检测。

IF 2.3

Journal of biophotonics Pub Date : 2025-08-28 DOI: 10.1002/jbio.202500221

Ishmael Apachigawo, Pradeep K Shukla, Dhruvil Solanki, Santanu Maity, Radhakrishna Rao, Prabhakar Pradhan

{"title":"Photonics Detection of Molecular-Specific Spatial Structural Alterations of Cells due to Chronic Alcoholism and Probiotics Treatments in Colon Cancer.","authors":"Ishmael Apachigawo, Pradeep K Shukla, Dhruvil Solanki, Santanu Maity, Radhakrishna Rao, Prabhakar Pradhan","doi":"10.1002/jbio.202500221","DOIUrl":"https://doi.org/10.1002/jbio.202500221","url":null,"abstract":"Photonics/light localization techniques are essential in understanding the structural changes in biological cells/tissues at the nano- to sub-micron scale. It is now known that structural alterations begin at the nanoscale, marking the onset of cancer progression. This photonics study examines the molecular-specific nano-structural alterations of chronic alcoholism and probiotic effects on colon cancer using a mouse model of colon cancer. We assessed alcohol-treated and azoxymethane (AOM) with dextran sulfate sodium (DSS)-induced colitis models, including ethanol (EtOH) and probiotic (L.casei) treatments separately and together. The confocal images were analyzed using the mesoscopic physics-based Inverse Participation Ratio (IPR) technique to quantify structural alterations at nano- to submicron scales. Significant enhancement of cancer progression was observed in the EtOH-treated group, and probiotic treatment with EtOH showed substantial reversal of these changes in colon cancer, underscoring the potential of the IPR technique in detecting and monitoring cancer progression.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500221"},"PeriodicalIF":2.3,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retinal Fluorescence Features in Alzheimer's Disease: In Vivo Study Using Synchronous Scan and Emission Mapping. 阿尔茨海默病视网膜荧光特征：使用同步扫描和发射作图的体内研究。

IF 2.3

Journal of biophotonics Pub Date : 2025-08-27 DOI: 10.1002/jbio.202500271

Sherif S Mahmoud, Rehab A Fouad, Eman M Aly

引用次数: 0

Hyperspectral Imaging for Distinguishing Granulomatous Mastitis From Normal Breast Tissue in Core Needle Biopsies. 高光谱成像在核心穿刺活检中鉴别肉芽肿性乳腺炎与正常乳腺组织。

IF 2.3

Journal of biophotonics Pub Date : 2025-08-27 DOI: 10.1002/jbio.202500240

Minmin Yu, Yukun Yin, Guangshang Zhong, Chanchan Zhu, Cuilei Wei, Mengqiu Zhang, Mengdi Zhang, Haiyue Lv, Xinqian Dong, Jingwei Li

引用次数: 0

Prognostic Value of Tumor Infiltrating Lymphocytes in Luminal B Breast Cancer Based on Multiphoton Microscopy. 基于多光子显微镜的B腔乳腺癌肿瘤浸润淋巴细胞的预后价值。

IF 2.3

Journal of biophotonics Pub Date : 2025-08-26 DOI: 10.1002/jbio.202500281

Xi Chen, Deyong Kang, Zhonghua Han, Jianping Huang, Zhen Lu, Jiajia He, Zhijun Li, Yuxuan Luo, Xingfu Wang, Lianhuang Li, Xiahui Han, Jianxin Chen, Liqin Zheng, Junzhen Jiang

{"title":"Prognostic Value of Tumor Infiltrating Lymphocytes in Luminal B Breast Cancer Based on Multiphoton Microscopy.","authors":"Xi Chen, Deyong Kang, Zhonghua Han, Jianping Huang, Zhen Lu, Jiajia He, Zhijun Li, Yuxuan Luo, Xingfu Wang, Lianhuang Li, Xiahui Han, Jianxin Chen, Liqin Zheng, Junzhen Jiang","doi":"10.1002/jbio.202500281","DOIUrl":"https://doi.org/10.1002/jbio.202500281","url":null,"abstract":"This study investigates the prognostic value of tumor-infiltrating lymphocytes (TILs) in luminal B breast cancer patients using multiphoton microscopy (MPM) and evaluates their association with 5-year disease-free survival (DFS). We obtained unlabeled MPM images from 213 patients, quantified the frequency of TILs, and assigned a TILs-score to each patient using ridge regression analysis. In addition, compared with single CLI-score model, the nomogram model, which integrated the TILs-score model with CLI-score model, further enhanced the predictive power for the luminal B subgroup. Specifically, the area under the curve (AUC) for 5-year DFS increased from 0.740 to 0.830 and hazard ratio (HR) increased from 5.31 to 7.24 in the training cohort; in the validation cohort, AUC increased from 0.716 to 0.822 and HR increased from 3.08 to 5.01. These findings suggest that the MPM-based TILs-score is a robust prognostic factor for luminal B breast cancer, potentially guiding more tailored treatment protocols.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500281"},"PeriodicalIF":2.3,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical Detection of the Spatial Structural Alteration in the Human Brain Tissues/Cells and DNA/Chromatin due to Parkinson's Disease. 帕金森病引起的人脑组织/细胞和DNA/染色质空间结构改变的光学检测

IF 2.3

Journal of biophotonics Pub Date : 2025-08-25 DOI: 10.1002/jbio.202500114

Fatemah Alharthi, Dhruvil Solanki, Ishmael Apachigawo, Santanu Maity, Jianfeng Xiao, Mohammad Moshahid Khan, Prabhakar Pradhan

{"title":"Optical Detection of the Spatial Structural Alteration in the Human Brain Tissues/Cells and DNA/Chromatin due to Parkinson's Disease.","authors":"Fatemah Alharthi, Dhruvil Solanki, Ishmael Apachigawo, Santanu Maity, Jianfeng Xiao, Mohammad Moshahid Khan, Prabhakar Pradhan","doi":"10.1002/jbio.202500114","DOIUrl":"10.1002/jbio.202500114","url":null,"abstract":"Parkinson's disease (PD) is one of the most common neurodegenerative disorders, highlighting the urgent need for early, reliable biomarkers. Structural disorder at the subcellular level, particularly in nuclear components such as DNA/chromatin, offers a promising diagnostic target. In this study, we applied two mesoscopic physics-based optical techniques-partial wave spectroscopy (PWS) and inverse participation ratio (IPR)-to quantify nanoscale structural alterations in postmortem human brain tissues and nuclei. Both PWS and IPR revealed a significant increase in structural disorder and mass density fluctuations in DNA/chromatin of PD samples. These abnormalities are potentially linked to the pathological aggregation of alpha-synuclein in the substantia nigra, a hallmark of PD-related neurodegeneration. Complementary histological analyses supported the optical findings, validating the presence of disrupted microarchitecture. Our results establish PWS and IPR as sensitive optical/photonics tools for detecting early nanoscale changes in PD, offering a novel path toward improved diagnosis and understanding of disease progression.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70131"},"PeriodicalIF":2.3,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light-Mediated Insulin and Amylin Secretion: Influence of Irradiation Parameters in Optogenetically Modified Mouse-Pancreatic β Cells. 光介导的胰岛素和胰淀素分泌：光基因修饰小鼠胰腺β细胞辐照参数的影响

IF 2.3

Journal of biophotonics Pub Date : 2025-08-24 DOI: 10.1002/jbio.202500232

Nidhi Maharwal, Rashmi Shrivastava, Khageswar Sahu, Shovan Kumar Majumder

{"title":"Light-Mediated Insulin and Amylin Secretion: Influence of Irradiation Parameters in Optogenetically Modified Mouse-Pancreatic β Cells.","authors":"Nidhi Maharwal, Rashmi Shrivastava, Khageswar Sahu, Shovan Kumar Majumder","doi":"10.1002/jbio.202500232","DOIUrl":"https://doi.org/10.1002/jbio.202500232","url":null,"abstract":"Optogenetics offers a promising avenue for controlled insulin secretion in diabetes management using genetically modified cells. However, the influence of irradiation parameters on the co-secreted hormone amylin remains largely unexplored. This study examined how blue light power densities (50, 100, and 200 mW/cm2) under continuous and intermittent exposure affect hormonal secretion in channelrhodopsin-2 expressing mouse β cells. Results showed that 10 s of continuous exposure at 50 mW/cm2 increased insulin and amylin secretion by ~25% and 40%, respectively. Introducing intermittent dark intervals further enhanced secretion by ~35% for insulin and 70% for amylin at 50 and 100 mW/cm2 respectively, correlating with intracellular calcium changes. Notably, light modulated the amylin-to-insulin molar ratio, peaking with a 34% increase during 5 s 'on'-10s 'off' exposure at 100 mW/cm2. This study provides the first evidence that light irradiation parameters can differentially influence hormonal secretion from engineered cells, offering a potential for tailored cell-based diabetes therapies.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500232"},"PeriodicalIF":2.3,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic Value of Intravascular Optical Coherence Tomography in Patients With Coronary Heart Disease Undergoing Percutaneous Coronary Intervention. 血管内光学相干断层扫描对冠心病患者经皮冠状动脉介入治疗的预后价值。

IF 2.3

Journal of biophotonics Pub Date : 2025-08-23 DOI: 10.1002/jbio.202500184

Zhengya Fang, Suwen Zhu, Shaodong Zhang, Yingchun Liu, Yueyun Shen, Ye Zhang

引用次数: 0

Comparative Analysis of Reflectance Confocal Microscopy and Multiphoton Microscopy in Basal Cell Carcinoma Diagnosis. 反射共聚焦显微镜与多光子显微镜诊断基底细胞癌的比较分析。

IF 2.3

Journal of biophotonics Pub Date : 2025-08-19 DOI: 10.1002/jbio.202500216

Wenwen Zhong, Lingyang Long, Ying Feng, Xiahui Han, Jianxin Chen

引用次数: 0

Motion Artifact Correction in Deep-Tissue Three-Photon Fluorescence Microscopy Using Adaptive Optical Flow Learning With Transformer. 基于变压器自适应光流学习的深层三光子荧光显微镜运动伪影校正。

IF 2.3

Journal of biophotonics Pub Date : 2025-08-17 DOI: 10.1002/jbio.202500407

Yifei Li, Runnan Zhang, Keying Li, Yalun Wang, Mubin He, Jun Qian

{"title":"Motion Artifact Correction in Deep-Tissue Three-Photon Fluorescence Microscopy Using Adaptive Optical Flow Learning With Transformer.","authors":"Yifei Li, Runnan Zhang, Keying Li, Yalun Wang, Mubin He, Jun Qian","doi":"10.1002/jbio.202500407","DOIUrl":"https://doi.org/10.1002/jbio.202500407","url":null,"abstract":"Three-photon fluorescence microscopy (3PFM) enables high-resolution volumetric imaging in deep tissues but is often hindered by motion artifacts in dynamic physiological environments. Existing solutions, including surgical fixation and conventional image registration algorithms, frequently fail under intense and nonuniform motions, particularly in low-texture or highly deformed regions. To overcome these problems, we propose StabiFormer, a transformer-based optical flow learning network designed for robust motion correction. Central to StabiFormer is the stable-dynamic feature extractor, which captures interlayer dynamics to facilitate accurate image registration. Our validation across cerebrovascular and intestinal 3PFM datasets demonstrates that StabiFormer achieves near-zero displacement error relative to ground truth in brain vasculature. Furthermore, it enables artifact-free 3D visualization of intestinal macrophages and vasculature at 300 μm depth, a physiologically relevant depth for studying intestinal immune microvasculature. These results establish a noninvasive computational solution for motion-artifact-free volumetric imaging, paving the way for quantitative investigations in previously inaccessible dynamic organ systems.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500407"},"PeriodicalIF":2.3,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0