Journal of biophotonics最新文献_第3页

Urine-Based FTIR Spectroscopy and Machine Learning Enable Non-Invasive Kidney Cancer Detection. 基于尿液的FTIR光谱和机器学习使无创肾癌检测成为可能。

IF 2.3

Journal of biophotonics Pub Date : 2026-04-01 DOI: 10.1002/jbio.70267

Przemysław Mitura, Adrian Odrzywolski, Olga Szyszkowska, Ewelina Gumbarewicz, Iga Kuliniec, Michał Godzisz, Andrzej Jadłoś, Joanna Depciuch

{"title":"Urine-Based FTIR Spectroscopy and Machine Learning Enable Non-Invasive Kidney Cancer Detection.","authors":"Przemysław Mitura, Adrian Odrzywolski, Olga Szyszkowska, Ewelina Gumbarewicz, Iga Kuliniec, Michał Godzisz, Andrzej Jadłoś, Joanna Depciuch","doi":"10.1002/jbio.70267","DOIUrl":"10.1002/jbio.70267","url":null,"abstract":"This study investigates the potential of Fourier transform infrared (FTIR) spectroscopy combined with multivariate and machine-learning analysis for kidney cancer detection using urine samples. FTIR analysis revealed distinct biochemical differences between urine from healthy controls and kidney cancer patients, with significant alterations observed in bands related to NH stretching, CH stretching, protein and urea-associated vibrations, phosphates, and carbohydrate-related regions. Principal component analysis showing group separation primarily along PC1 (33.9% variance), while uniform manifold approximation and projection provided enhanced nonlinear discrimination. Multiple models achieving high classification performance. With first-derivative preprocessing, both random forest and support vector machines reached 100% accuracy (sensitivity: 100%, 95% CI: 92.7%-100%; specificity: 100%, 95% CI: 91.2%-100%; AUC: 1.000 (95% CI: 1.000-1.000)). With raw spectral data, random forest achieved 98.9% accuracy (sensitivity: 100%, 95% CI: 92.7%-100%; specificity: 97.5%, 95% CI: 87.1%-99.6%; AUC: 1.000). Feature stability identified reproducible wavenumbers as cancer biomarkers.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 4","pages":"e70267"},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147694533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Second Harmonic Generation-Based Collagen Analysis and Automated Grading of Myelofibrosis. 基于二次谐波生成的胶原蛋白分析和骨髓纤维化自动分级。

IF 2.3

Journal of biophotonics Pub Date : 2026-04-01 DOI: 10.1002/jbio.70269

Xunbin Yu, Guodong Guo, Xia Zhang, Xin Chen, Xingxin Huang, Lianhuang Li

引用次数: 0

Therapeutic Effect of Photobiomodulation and Single Intratendinous Injection at the Lesion Site of Platelet-Rich Fibrin in Inducted Tendinopathy. 光生物调节和单次肌腱内注射富血小板纤维蛋白对诱导性肌腱病变的治疗作用。

IF 2.3

Journal of biophotonics Pub Date : 2026-04-01 DOI: 10.1002/jbio.70271

Caroline Hammerschmitt Wild, Pâmela Andressa Pauletto, Fransael Franklyn Araújo da Silva, Maria Eduarda Luckner, Gladson Ricardo Flor Bertolini

{"title":"Therapeutic Effect of Photobiomodulation and Single Intratendinous Injection at the Lesion Site of Platelet-Rich Fibrin in Inducted Tendinopathy.","authors":"Caroline Hammerschmitt Wild, Pâmela Andressa Pauletto, Fransael Franklyn Araújo da Silva, Maria Eduarda Luckner, Gladson Ricardo Flor Bertolini","doi":"10.1002/jbio.70271","DOIUrl":"10.1002/jbio.70271","url":null,"abstract":"Tendinopathy is a multifactorial condition characterized by collagen disorganization, inflammation, and impaired tendon function. Photobiomodulation (PBM) and platelet-rich fibrin (PRF) have emerged as therapeutic approaches due to their regenerative potential, although their mechanisms during tendon healing remain unclear. This study compared the effects of PBM and PRF, alone or combined, on experimentally induced calcaneal tendinopathy in rats. A total of 75 animals were divided into five groups: control, injury, PRF, PBM, and PRF + PBM. Treatments began 48 h after injury. Functional performance, nociception, and histological features were evaluated up to 21 days. Combined PRF and PBM promoted superior early functional recovery, enhanced collagen synthesis, and increased angiogenesis, despite greater initial inflammation. PBM alone resulted in gradual recovery with better fiber organization, while PRF accelerated function but sustained inflammation. Combined therapy shows promise for tendon repair, supporting stage-specific treatment strategies.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 4","pages":"e70271"},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13092645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147725108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functionalized Quantum Dot-Based Portable Sensor for Dual Detection of Stress Biomarkers in Blood Serum and Saliva. 基于功能化量子点的便携式传感器在血清和唾液中双重检测应激生物标志物。

IF 2.3

Journal of biophotonics Pub Date : 2026-04-01 DOI: 10.1002/jbio.70268

Anusha Kishore, Arun Mathew Varughese, Bernhard Roth, Carsten Zeilinger

{"title":"Functionalized Quantum Dot-Based Portable Sensor for Dual Detection of Stress Biomarkers in Blood Serum and Saliva.","authors":"Anusha Kishore, Arun Mathew Varughese, Bernhard Roth, Carsten Zeilinger","doi":"10.1002/jbio.70268","DOIUrl":"10.1002/jbio.70268","url":null,"abstract":"This work reports on the simultaneous detection of cellular stress biomarkers in blood and saliva, using a portable sensor. We expanded the application of our optical measurement system, the Transilluminated Quantum Dot (TIQD) imager, paired with a low-cost biochip for minimally invasive biomolecule detection. Streptavidin functionalized quantum dots (Sav-QDs) were conjugated with biotinylated antibodies (Ab) against stress biomarkers heat shock protein (Hsp). The presence of biomarkers in biological samples was indicated by the fluorescence masking of Sav-QDs, based on their binding to the Abs conjugated to Sav-QDs, resulting in reduced fluorescence. The decrease in fluorescence directly correlates with biomarker concentration. We have successfully detected Hsp90 and Hsp70 spiked in artificial blood serum. The limit of detection (LoD) was around 50 nM for Hsp90 and 96 nM for Hsp70. Our portable sensor addresses the need for reliable point-of-care (PoC) stress diagnosis by enabling robust, multiple biomarkers detection in diverse biological samples.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 4","pages":"e70268"},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13092650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147725078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding Bioenergetics of Myogenic Differentiation of Human Mesenchymal Stem Cells Using Label-Free Imaging. 利用无标记成像技术了解人间充质干细胞肌源性分化的生物能量学。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70256

Vrushali Khedekar, Gayathri S Kamath, Indrani Talukdar, P Nandakumar, Geetha K Varier

引用次数: 0

Precise Multiscale Registration and Anti-Shaking Algorithm for Stable Nailfold Vascular Area Visualization. 精确多尺度配准及抗震动甲襞血管区域稳定可视化算法。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70257

Jianan Lin, Bin Zhou, Yanxiong Wu, Peiqing Guo, Jiaxiong Luo, Yaozhang Huang, Shou Feng, Qirui Sun, Xiaosong Li, Wei Bin, Zhiming Yang

{"title":"Precise Multiscale Registration and Anti-Shaking Algorithm for Stable Nailfold Vascular Area Visualization.","authors":"Jianan Lin, Bin Zhou, Yanxiong Wu, Peiqing Guo, Jiaxiong Luo, Yaozhang Huang, Shou Feng, Qirui Sun, Xiaosong Li, Wei Bin, Zhiming Yang","doi":"10.1002/jbio.70257","DOIUrl":"10.1002/jbio.70257","url":null,"abstract":"In nailfold capillary imaging, tremors cause image blurring and prevent accurate measurement, while capillary bundle distributions can diminish vascular information. As a comprehensive solution, we combined the YOLOv8 detection model with a Laplacian clarity evaluation function for real-time tracking of focus changes during capillary imaging. Normalized cross-correlation template matching was used to calculate the optimal matching position of adjacent frames, determine the position with the highest vascular similarity, and obtain the optimal offset between frames for accurate registration. Compared with the original video, mean squared error (MSE) decreased by 64.5%, peak signal-to-noise ratio (PSNR) increased by 3.88 dB, and structural similarity index (SSIM) increased by 4.7% in zooming experiments. MSE decreased by 57.9%, PSNR increased by 3.99 dB, and SSIM increased by 3.19% in fixed-focus experiments. Compared with state-of-the-art registration algorithms, our proposal provides better overall performance across three evaluation indicators, achieving the best registration effect for capillary images.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70257"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147523243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative Treatment for Reducing Persistent Populations of Escherichia coli Under Amoxicillin and Gentamicin Pressure. 氧化处理在阿莫西林和庆大霉素压力下减少大肠杆菌的持久性种群。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 Epub Date: 2025-12-23 DOI: 10.1002/jbio.70212

Bruna C Correa, Maria Vitória S Pereira, Vanderlei S Bagnato, Kate C Blanco

{"title":"Oxidative Treatment for Reducing Persistent Populations of Escherichia coli Under Amoxicillin and Gentamicin Pressure.","authors":"Bruna C Correa, Maria Vitória S Pereira, Vanderlei S Bagnato, Kate C Blanco","doi":"10.1002/jbio.70212","DOIUrl":"10.1002/jbio.70212","url":null,"abstract":"Bacterial persistence is a transient phenotypic state in which a subpopulation of cells survives antibiotic exposure without genetic resistance. These dormant, low-metabolic cells are linked to recurrent infections and reduced antibiotic efficacy. Photodynamic inactivation (PDI), which generates reactive oxygen species (ROS) through photosensitizer activation under visible light, is a promising strategy to counteract persistence. This study evaluated PDI effects on Escherichia coli under amoxicillin and gentamicin pressure, using time-kill assays and MDK99/MDK99.99 metrics. PDI, optimized with methylene blue (10 μM) and 10 J/cm2 light at 660 nm, was applied to antibiotic-exposed cells and progeny. For amoxicillin, PDI reduced MDK99 from > 3 to 2 h; for gentamicin, it suppressed regrowth in progeny and reduced MDK99.99 from 3 to 2 h. Scanning electron microscopy showed morphological damage consistent with persistence. PDI enhanced antibiotic efficacy and shortened treatment time, supporting further investigation of PDI-antibiotic combinations for chronic infections.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70212"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12980568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145822547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparative Study on Signal Decomposition Techniques for Stimulated Raman Photoacoustic Microscopy. 受激拉曼光声显微镜信号分解技术的比较研究。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 Epub Date: 2025-12-15 DOI: 10.1002/jbio.202500388

Amir Khansari, Hossein Khodavirdi, Seyed Mohsen Ranjbaran, Mohsin Zafar, Mohammad Javad Beirami, Kamran Avanaki

{"title":"A Comparative Study on Signal Decomposition Techniques for Stimulated Raman Photoacoustic Microscopy.","authors":"Amir Khansari, Hossein Khodavirdi, Seyed Mohsen Ranjbaran, Mohsin Zafar, Mohammad Javad Beirami, Kamran Avanaki","doi":"10.1002/jbio.202500388","DOIUrl":"10.1002/jbio.202500388","url":null,"abstract":"In this paper, we present a comparative study of signal decomposition techniques in multispectral photoacoustic microscopy (MS-PAM) systems that are based on the use of stimulated Raman spectroscopy (SRS). The signal decomposition algorithms leverage the inherent delay between the arrival of two photoacoustic (PA) signals, one from 532 nm and the other from 558 nm. We evaluate four signal decomposition methods: fast Fourier transform (FFT), least squares (LSQ), cross-correlation (XCorr), and a deep learning (DL) approach based on a convolutional neural network (CNN) coupled with an autoencoder architecture. These methods are tested against ground truth PA signals generated separately at 532 and 558 nm wavelengths from the mouse brain. Results show that the FFT method consistently outperforms the others in terms of accuracy, while LSQ, XCorr, and DL's performance exhibits greater variability, especially at longer time delays. Finally, the performance of oxygen saturation estimation is evaluated using the best method (FFT).","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70184"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12976986/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145764852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Potential of Photobiomodulation in Early Recovery After Experimental Spinal Cord Injury in Rats: Histological and Biomechanical Analysis. 光生物调节在实验性脊髓损伤早期恢复中的治疗潜力：组织学和生物力学分析。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 Epub Date: 2025-12-15 DOI: 10.1002/jbio.202500348

Débora Campos Chaves Correia, Leonardo Borges de Lima, Mário Oliveira Lima, Luis Filipe Karatanasov Beloni, Raduan Hage, Emilia Angela Lo Schiavo Arisawa

{"title":"Therapeutic Potential of Photobiomodulation in Early Recovery After Experimental Spinal Cord Injury in Rats: Histological and Biomechanical Analysis.","authors":"Débora Campos Chaves Correia, Leonardo Borges de Lima, Mário Oliveira Lima, Luis Filipe Karatanasov Beloni, Raduan Hage, Emilia Angela Lo Schiavo Arisawa","doi":"10.1002/jbio.202500348","DOIUrl":"10.1002/jbio.202500348","url":null,"abstract":"Spinal cord injury (SCI) leads to severe functional deficits, underscoring the critical need for new therapies. This study evaluated the efficacy of photobiomodulation (PBM) as an early, noninvasive treatment for induced SCI, using specific parameters (808 nm, 72 J/cm2, 100 mW). A total of 15 rats were divided into Control (C), SCI, and PBM groups. Efficacy was determined by an integrated approach, correlating ground reaction force (GRF) with quantitative histological assessment. The PBM group showed a significant reduction of secondary damage (33.3 ± 5.5 vs. 53.9 ± 7.0 in the SCI group; p = 0.0002) and preserved neural structure. This tissue preservation aligns with the GRF analysis, which demonstrated that the PBM group recovered gait patterns similar to the Control group. In conclusion, PBM effectively mitigates necrosis aerea, maintaining tissue integrity, improving functional recovery, and reinforcing the PBM's therapeutic potential as a promising translatable strategy for outcomes after SCI. Further research should include immunostaining of cells and larger samples.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500348"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12980696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145764837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical Heterogeneity of Endometriosis Phenotypes Revealed by FTIR Analysis. FTIR分析揭示子宫内膜异位症表型的生化异质性。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 Epub Date: 2025-11-30 DOI: 10.1002/jbio.202500511

Piotr Olcha, Wiesław Paja, Michał Kępski, Krzysztof Pancerz, Bartosz Klebowski, Łukasz Nowakowski, Krzysztof Gałczyński, Joanna Depciuch

{"title":"Biochemical Heterogeneity of Endometriosis Phenotypes Revealed by FTIR Analysis.","authors":"Piotr Olcha, Wiesław Paja, Michał Kępski, Krzysztof Pancerz, Bartosz Klebowski, Łukasz Nowakowski, Krzysztof Gałczyński, Joanna Depciuch","doi":"10.1002/jbio.202500511","DOIUrl":"10.1002/jbio.202500511","url":null,"abstract":"Fourier-transform infrared (FTIR) spectroscopy was used to investigate molecular differences among peritoneal, ovarian, bowel endometriosis phenotypes and control tissues. Peritoneal lesions showed the most pronounced spectral changes in CH-stretching (2800-3000 cm-1) and 1000-1500 cm-1 regions, indicating protein and lipid alterations. Bowel lesions exhibited moderate but significant deviations, particularly in protein- and nucleic acid-associated bands, while ovarian lesions displayed subtler differences with increased lipid-related CH-stretching. Principal component analysis distinguished all phenotypes from controls, with peritoneal clustering most distinct, bowel intermediate, and ovarian partially overlapping. Key discriminative regions included phosphate vibrations (1080-1100, 1240-1250 cm-1), CH2 bending (~1450 cm-1), amide I (~1650 cm-1), and amide II (~1540 cm-1). Decision tree analysis identified phenotype-specific markers: 988 cm-1 (ovarian), 1101 cm-1 (bowel), 1544 cm-1 (peritoneal). Marker intensity correlated with tumor size and clinical scores, strongest in ovarian lesions. These findings highlight phenotype-specific FTIR fingerprints, offering diagnostic and prognostic potential in endometriosis.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500511"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145650621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0