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Journal of biophotonics最新文献

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Digital Cytometry: Extraction of Forward and Side Scattering Signals From Holotomography. 数字细胞测量：从 Holotomography 提取正向和侧向散射信号。
Journal of biophotonics Pub Date : 2025-02-05 DOI: 10.1002/jbio.202400387
Jaepil Jo, Herve Hugonnet, Mahn Jae Lee, YongKeun Park
{"title":"Digital Cytometry: Extraction of Forward and Side Scattering Signals From Holotomography.","authors":"Jaepil Jo, Herve Hugonnet, Mahn Jae Lee, YongKeun Park","doi":"10.1002/jbio.202400387","DOIUrl":"https://doi.org/10.1002/jbio.202400387","url":null,"abstract":"<p><p>Flow cytometry is a cornerstone technique in medical and biological research, providing crucial information about cell size and granularity through forward scatter (FSC) and side scatter (SSC) signals. Despite its widespread use, the precise relationship between these scatter signals and corresponding microscopic images remains underexplored. Here, we investigate this intrinsic relationship by utilizing scattering theory and holotomography, a three-dimensional quantitative phase imaging (QPI) technique. We demonstrate the extraction of FSC and SSC signals from individual, unlabeled cells by analyzing their three-dimensional refractive index distributions obtained through holotomography. Additionally, we introduce a method for digital windowing of SSC signals to facilitate effective segmentation and morphology-based cell type classification. Our approach bridges the gap between flow cytometry and microscopic imaging, offering a new perspective on analyzing cellular characteristics with high accuracy and without the need for labeling.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400387"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced Fluorescence Imaging of Implants Based on Polyester Copolymers in Combination With MRI. 基于聚酯共聚物的植入物与MRI联合增强荧光成像。
Journal of biophotonics Pub Date : 2025-02-03 DOI: 10.1002/jbio.202400147
Victoria V Zherdeva, Astemir R Likhov, Ulugbek A Saidvaliev, Dror Fixler, Dmitry Demin, Veronika N Volodina, Uliana A Apukhtina, Shweta Pawar, Bar Atuar, Valery V Tuchin
{"title":"Enhanced Fluorescence Imaging of Implants Based on Polyester Copolymers in Combination With MRI.","authors":"Victoria V Zherdeva, Astemir R Likhov, Ulugbek A Saidvaliev, Dror Fixler, Dmitry Demin, Veronika N Volodina, Uliana A Apukhtina, Shweta Pawar, Bar Atuar, Valery V Tuchin","doi":"10.1002/jbio.202400147","DOIUrl":"https://doi.org/10.1002/jbio.202400147","url":null,"abstract":"<p><p>Nowadays, many biodegradable materials are offered for biomedical applications, but there are only a few in vivo methods for their detection and monitoring. In this work, implants based on biodegradable polyester copolymers were labeled with indocyanine green (ICG) for fluorescence imaging in combination with tissue optical clearing (TOC) and magnetic resonance imaging (MRI). The results include in vitro degradation modeling followed by in vivo imaging of copolymer samples that were subcutaneously implanted in BALB/c mice. TOC with 70% glycerol has been demonstrated to significantly improve sample visualization. The TOC efficiency parameter Q demonstrated the variability of effects correlating with the timing of follow-up in the postimplantation period. It has been shown that nonhealing wounds, peri-implantation inflammation, or fibrosis, confirmed by MRI, affect the effectiveness of TOC in the range from Q = -30% to 70%.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400147"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diabetes Mellitus Impairs Blood-Brain Barrier Integrality and Microglial Reactivity. 糖尿病损害血脑屏障完整性和小胶质细胞反应性。
Journal of biophotonics Pub Date : 2025-01-27 DOI: 10.1002/jbio.202400482
Shaojun Liu, Jie Hao, Tingting Yu, Valery V Tuchin, Junming Li, Dongyu Li, Dan Zhu
{"title":"Diabetes Mellitus Impairs Blood-Brain Barrier Integrality and Microglial Reactivity.","authors":"Shaojun Liu, Jie Hao, Tingting Yu, Valery V Tuchin, Junming Li, Dongyu Li, Dan Zhu","doi":"10.1002/jbio.202400482","DOIUrl":"https://doi.org/10.1002/jbio.202400482","url":null,"abstract":"<p><p>Diabetes mellitus (DM), a chronic metabolic disorder that adversely affects the blood-brain barrier (BBB) and microglial function in the central nervous system (CNS), contributing to neuronal damage and neurodegenerative diseases. However, the underlying molecular mechanisms linking diabetes to BBB dysfunction and microglial dysregulation remain poorly understood. Here, we assessed the impacts of diabetes on BBB and microglial reactivity and investigated its mechanisms. We found diabetes severely disrupted the BBB integrity and microglial response to vascular injury. We also revealed a potential relationship between BBB disruption and impaired microglial function, whereby increasing BBB permeability led to a downregulation of microglial P2RY12 expression, thereby impairing microglial protection against cerebrovascular injury. Understanding these mechanisms may contribute to the developing of therapeutic strategies for diabetes-related neurological complications.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400482"},"PeriodicalIF":0.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optical Based Methods for Water Monitoring in Biological Tissue. 生物组织中水分监测的光学方法。
Journal of biophotonics Pub Date : 2025-01-24 DOI: 10.1002/jbio.202400438
Sergei Perkov, Julijana Cvjetinovic, Altynai Sydygalieva, Sergey Gorodkov, Guoqiang Li, Dmitry Gorin
{"title":"Optical Based Methods for Water Monitoring in Biological Tissue.","authors":"Sergei Perkov, Julijana Cvjetinovic, Altynai Sydygalieva, Sergey Gorodkov, Guoqiang Li, Dmitry Gorin","doi":"10.1002/jbio.202400438","DOIUrl":"https://doi.org/10.1002/jbio.202400438","url":null,"abstract":"<p><p>Skin homeostasis is strongly dependent on its hydration levels, making skin water content measurement vital across various fields, including medicine, cosmetology, and sports science. Noninvasive diagnostic techniques are particularly relevant for clinical applications due to their minimal risk of side effects. A range of optical methods have been developed for this purpose, each with unique physical principles, advantages, and limitations. This review provides an in-depth examination of optical techniques such as diffuse reflectance spectroscopy, optoacoustic spectroscopy, optoacoustic tomography, hyperspectral imaging, and Raman spectroscopy. We explore their efficacy in noninvasive monitoring of skin hydration and edema, which is characterized by an increase in interstitial fluid. By comparing the parameters, sensitivity, and clinical applications of these techniques, this review offers a comprehensive understanding of their potential to enhance diagnostic precision and improve patient care.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400438"},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of Microcirculation and Microrheological Parameters of Blood in Patients With Type 2 Diabetes Mellitus Using Biophotonics Techniques. 生物光子学技术评价2型糖尿病患者血液微循环和微流变参数。
Journal of biophotonics Pub Date : 2024-12-16 DOI: 10.1002/jbio.202400485
D A Umerenkov, P B Ermolinskiy, A E Lugovtsov, A A Fabrichnova, Yu I Gurfinkel, L I Dyachuk, A V Priezzhev
{"title":"Assessment of Microcirculation and Microrheological Parameters of Blood in Patients With Type 2 Diabetes Mellitus Using Biophotonics Techniques.","authors":"D A Umerenkov, P B Ermolinskiy, A E Lugovtsov, A A Fabrichnova, Yu I Gurfinkel, L I Dyachuk, A V Priezzhev","doi":"10.1002/jbio.202400485","DOIUrl":"https://doi.org/10.1002/jbio.202400485","url":null,"abstract":"<p><p>In this study, the parameters of blood microcirculation and microrheology were measured using the methods of laser aggregometry and optical tweezers in vitro, as well as the method of digital capillaroscopy in vivo. It was shown that in patients suffering from type 2 diabetes mellitus, an increase in the number of RBC aggregates passing through the narrow capillaries leads to a significant decrease in the velocity of the capillary blood flow, which can be explained by the increased viscosity of the whole blood and decreased deformability of RBCs. Also, for the group of patients, a statistically significant increase in the rate of RBC aggregation and the hydrodynamic strength of aggregates, RBC aggregation and disaggregation forces were observed compared to the control group. We have demonstrated the possibility of using these methods to assess changes in microrheological and microcirculatory parameters of the blood.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400485"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Shear Wave Optical Coherence Elastography and Structural Analysis of the Postnatal Mouse Cornea Through Development. 出生后小鼠角膜发育的剪切波光学相干弹性成像及结构分析。
Journal of biophotonics Pub Date : 2024-12-02 DOI: 10.1002/jbio.202400331
Andrew L Lopez, Mohammad Dehshiri, Alexander W Schill, Salavat Aglyamov, Kirill Larin
{"title":"Shear Wave Optical Coherence Elastography and Structural Analysis of the Postnatal Mouse Cornea Through Development.","authors":"Andrew L Lopez, Mohammad Dehshiri, Alexander W Schill, Salavat Aglyamov, Kirill Larin","doi":"10.1002/jbio.202400331","DOIUrl":"10.1002/jbio.202400331","url":null,"abstract":"<p><p>The cornea is a transparent lens at the forefront of the eye, serving as a structural barrier that protects the eye and provides the majority of the eye's refractive power. In this study, we utilized the postnatal mouse eye to characterize corneal structure and biomechanics. Between postnatal day (PN) 6 and PN24, we observed that elastic wave speeds are highest at PN6, gradually decrease through PN24, and then start to rise in adulthood (6 months). We found that corneal thickness is uncoupled from elastic wave speed and that the content and organization of the cornea primarily influence its mechanical properties. To the best of our knowledge, this work is the first detailed assessment of the postnatal mouse cornea's structure and biomechanics and warrants further investigation into the dynamic properties of the postnatal eye.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400331"},"PeriodicalIF":0.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of 1267 nm Illumination on Microcirculation Regulatory Mechanisms. 1267 纳米光照对微循环调节机制的影响
Journal of biophotonics Pub Date : 2024-11-26 DOI: 10.1002/jbio.202400296
Lyubov Eratova, Irina Makovik, Viktor Dremin
{"title":"Effects of 1267 nm Illumination on Microcirculation Regulatory Mechanisms.","authors":"Lyubov Eratova, Irina Makovik, Viktor Dremin","doi":"10.1002/jbio.202400296","DOIUrl":"https://doi.org/10.1002/jbio.202400296","url":null,"abstract":"<p><p>This study explored the effects of 1267 nm laser irradiation on changes in blood flow parameters and activation of the regulatory mechanisms of the microcirculatory bed (MCB). Using laser Doppler flowmetry (LDF) technique and time-frequency analysis of perfusion signals, changes in the MCB of 16 healthy volunteers, targeting the distal phalanx of the third finger with 1267 nm laser irradiation were evaluated. Results indicated no significant differences in perfusion between control and target measurements, likely due to blood flow redistribution caused by vessel dilation/constriction. However, differences in oscillation amplitudes in endothelial and myogenic ranges were observed, suggesting microcirculation self-regulation. Detailed analysis revealed characteristic peaks in the endothelial range during and after irradiation, indicating endothelial mediator release. It is assumed that the identified effects may be related to the singlet oxygen generated by 1267 nm laser irradiation, which directly affects the MCB, manifesting as endothelium-dependent vascular activity.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400296"},"PeriodicalIF":0.0,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Estimation of Scattering Properties Modifications Caused by In Vivo Human Skin Optical Clearing Using Line-Field Confocal Optical Coherence Tomography. 利用线场共焦光学相干断层成像技术估算体内人体皮肤光学清除造成的散射特性改变。
Journal of biophotonics Pub Date : 2024-11-11 DOI: 10.1002/jbio.202400264
Sergey M Zaytsev, Léna Waszczuk, Jonas Ogien, Arnaud Dubois, Walter Blondel, Marine Amouroux
{"title":"Estimation of Scattering Properties Modifications Caused by In Vivo Human Skin Optical Clearing Using Line-Field Confocal Optical Coherence Tomography.","authors":"Sergey M Zaytsev, Léna Waszczuk, Jonas Ogien, Arnaud Dubois, Walter Blondel, Marine Amouroux","doi":"10.1002/jbio.202400264","DOIUrl":"https://doi.org/10.1002/jbio.202400264","url":null,"abstract":"<p><p>The image contrast and probing depth of optical methods applied to in vivo skin could be improved by reducing skin scattering using the optical clearing method. The aim of this study was to quantify, from line-field confocal optical coherence tomography (LC-OCT) 3D images, the modifications of skin scattering properties in vivo during optical clearing. Nine mixtures of optical clearing agents were used in combination with physical and chemical permeation enhancers on the human skin of three healthy volunteers. Scattering coefficient and anisotropy factor of the epidermis and the upper dermis were estimated from the 3D LC-OCT images of skin using an exponential decay model of the in-depth intensity profile. We were able to demonstrate a decrease in epidermal scattering (down to 33%) related to optical clearing, with the best results obtained by a mixture of polyethylene glycol, oleic acid, and propylene glycol.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400264"},"PeriodicalIF":0.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Developmental Imaging of Radish Sprouts Using Dynamic Optical Coherence Tomography. 利用动态光学相干断层扫描技术对萝卜芽进行发育成像。
Journal of biophotonics Pub Date : 2024-11-05 DOI: 10.1002/jbio.202400254
Yiheng Lim, Shumpei Kojima, Pradipta Mukherjee, Ibrahim Abd El-Sadek, Shuichi Makita, Yoshiaki Yasuno
{"title":"Developmental Imaging of Radish Sprouts Using Dynamic Optical Coherence Tomography.","authors":"Yiheng Lim, Shumpei Kojima, Pradipta Mukherjee, Ibrahim Abd El-Sadek, Shuichi Makita, Yoshiaki Yasuno","doi":"10.1002/jbio.202400254","DOIUrl":"https://doi.org/10.1002/jbio.202400254","url":null,"abstract":"<p><p>The germination process of radish sprouts was investigated in detail using volumetric dynamic optical coherence tomography (OCT). Dynamic OCT involves the sequential acquisition of 16 OCT images and subsequent temporal variance analysis of each pixel, enabling non-invasive visualization of the cellular and tissue activities of plants. The radish sprouts were longitudinally investigated for up to 12 days, and changes in morphology and dynamic OCT image patterns were observed as the plants developed. The dynamic OCT signals in the vessels and growing roots were relatively high in the early stage of germination and decreased as the tissue matured. These results suggest that dynamic OCT is sensitive to water and nutrient transport as well as cellular activities associated with plant growth.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400254"},"PeriodicalIF":0.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of FOLFOX Chemotherapy on Tumor Oxygenation and Perfused Vasculature: An In Vivo Study by Optical Techniques. FOLFOX 化疗对肿瘤氧合和灌注血管的影响：利用光学技术进行的体内研究。
Journal of biophotonics Pub Date : 2024-10-29 DOI: 10.1002/jbio.202400339
Irina Druzhkova, Kseniya Bylinskaya, Anton Plekhanov, Alexey Kostyuk, Mikhail Kirillin, Valeriya Perekatova, Aleksandr Khilov, Anna Orlova, Anastasiya Polozova, Anastasiya Komarova, Uliyana Lisitsa, Marina Sirotkina, Marina Shirmanova, Ilya Turchin
{"title":"Effects of FOLFOX Chemotherapy on Tumor Oxygenation and Perfused Vasculature: An In Vivo Study by Optical Techniques.","authors":"Irina Druzhkova, Kseniya Bylinskaya, Anton Plekhanov, Alexey Kostyuk, Mikhail Kirillin, Valeriya Perekatova, Aleksandr Khilov, Anna Orlova, Anastasiya Polozova, Anastasiya Komarova, Uliyana Lisitsa, Marina Sirotkina, Marina Shirmanova, Ilya Turchin","doi":"10.1002/jbio.202400339","DOIUrl":"https://doi.org/10.1002/jbio.202400339","url":null,"abstract":"<p><p>The effects of cytotoxic chemotherapy on tumor vasculature and oxygenation are in the focus of modern investigations because vascular structure and distribution of oxygen influence tumor behavior and treatment response. The aim of our study was to monitor changes in the vascular component of colorectal tumor xenografts induced by a clinical combination of chemotherapy drugs FOLFOX in vivo using two complementary techniques: diffuse reflectance spectroscopy (DRS) and optical coherence tomography-based microangiography (OCT-MA). These techniques revealed a slower decrease in tumor blood oxygenation in treated tumors as compared to untreated ones, faster suppression of tumor vasculature perfusion and increase in water content as a result of treatment, and decrease in total hemoglobin in untreated tumors. Immunohistochemical analysis of hypoxia-inducible factor HIF-2α detected tissue hypoxia as a consequence of inappropriate oxygen supply in the treated tumors. The obtained results show the prospects for monitoring of treatment efficacy using DRS and OCT-MA.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400339"},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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