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Scattering Reduction and Axial Resolution Enhancement in Light-Sheet Fluorescence Microscopy.
Journal of biophotonics Pub Date : 2025-02-23 DOI: 10.1002/jbio.202400556
Yang Zhang, Runze Li, Xianghua Yu, Hao Miao, Ruiwen Yang, Xing Li, Junwei Min, Yanlong Yang, Dan Dan, Taiqiang Dai, Liang Kong, Baoli Yao
{"title":"Scattering Reduction and Axial Resolution Enhancement in Light-Sheet Fluorescence Microscopy.","authors":"Yang Zhang, Runze Li, Xianghua Yu, Hao Miao, Ruiwen Yang, Xing Li, Junwei Min, Yanlong Yang, Dan Dan, Taiqiang Dai, Liang Kong, Baoli Yao","doi":"10.1002/jbio.202400556","DOIUrl":"https://doi.org/10.1002/jbio.202400556","url":null,"abstract":"<p><p>Light-sheet fluorescence microscopy (LSFM) provides an ideal tool for long-term observation of live specimens due to its low photodamage and fast volumetric imaging speed. The wavefront distortions in the illumination path of LSFM will reduce the intensity and broaden the light-sheet thickness, thereby degrading the image quality. We propose to use the wavefront shaping technique to reduce the scattering effect and shrink the light-sheet thickness. Scanning the refocused laser beam to generate LS improves both the fluorescence intensity and the axial resolution. The axial resolution can be further enhanced by subtracting the two images captured via double scanning the samples with the refocused beam and the uncorrected scattered beam for each slice. The axial resolution is improved from 2.2 ± 0.3 to 1.5 ± 0.2 μm across the field of view of 270 μm × 270 μm. The effectiveness of the wavefront shaping subtraction method is demonstrated by imaging fluorescent beads and Aspergillus conidiophores behind a scattering medium.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400556"},"PeriodicalIF":0.0,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spectral Differentiation of Esophageal Precancerous Lesion Staging and an Improved Feature Wavelength Selection Method Based on Enhanced Fox Algorithm.
Journal of biophotonics Pub Date : 2025-02-23 DOI: 10.1002/jbio.202400518
Jinbao Zhang, Shuangli Liu, Fanrong Wang, Li Wang, Jiamin Qin, Liming Wen, Weijia Wan
{"title":"Spectral Differentiation of Esophageal Precancerous Lesion Staging and an Improved Feature Wavelength Selection Method Based on Enhanced Fox Algorithm.","authors":"Jinbao Zhang, Shuangli Liu, Fanrong Wang, Li Wang, Jiamin Qin, Liming Wen, Weijia Wan","doi":"10.1002/jbio.202400518","DOIUrl":"https://doi.org/10.1002/jbio.202400518","url":null,"abstract":"<p><p>Near-infrared (NIR) spectroscopy, known for its non-destructive, rapid, and precise nature, captures spectral responses to chemical bond changes in cancerous tissues. This provides a promising approach for accurate cancer staging and identifying spectral differences between cancerous and healthy tissues. In this study, NIR data from esophageal lesions excised via endoscopic submucosal dissection were analyzed using partial least squares discriminant analysis (PLS-DA) to classify normal tissues, low-grade, and high-grade intraepithelial neoplasia, confirming its feasibility for staging diagnosis. To enhance wavelength selection, the FOX algorithm, a swarm intelligence optimization method, is improved with two modifications: a nonlinear time-varying sigmoid transfer function and mirror selection. These enhancements are combined to form an improved FOX algorithm (iFOX) for wavelength selection. iFOX effectively enhances the algorithm's stability while enhancing classification performance.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400518"},"PeriodicalIF":0.0,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
External Validation of Raman Spectroscopy for Lyme Disease Diagnostics.
Journal of biophotonics Pub Date : 2025-02-20 DOI: 10.1002/jbio.202400520
Isaac D Juárez, Aidan P Holman, Elizabeth J Horn, Artem S Rogovskyy, Dmitry Kurouski
{"title":"External Validation of Raman Spectroscopy for Lyme Disease Diagnostics.","authors":"Isaac D Juárez, Aidan P Holman, Elizabeth J Horn, Artem S Rogovskyy, Dmitry Kurouski","doi":"10.1002/jbio.202400520","DOIUrl":"https://doi.org/10.1002/jbio.202400520","url":null,"abstract":"<p><p>Lyme disease (LD), caused by Borreliella burgdorferi, is the most common tick-borne illness in the United States, yet early-stage diagnosis remains challenging due to the limitations of current serological diagnostics. Raman spectroscopy (RS), paired with partial least squares discriminant analysis (PLS-DA), showed promise as an alternative diagnostic tool. Using RS, we analyzed 107 coded human blood samples (42 LD-positive and 65 LD-negative) obtained from the Lyme Disease Biobank. PLS-DA models showed nearly perfect internal validation performance with a sensitivity and specificity of 97.1% and 100.0%, respectively, indicating robust predictive capabilities. External validation of the developed chemometrics model with 80/20 training/validation split of all spectra gave true positive rates of 92.7% and 87.3% for serological positive and negative spectra, respectively. These findings highlight the potential of RS as a rapid and noninvasive diagnostic platform for LD, particularly when integrated with machine learning.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400520"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143470437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and Validation of a Noninvasive Diffuse Reflectance Spectroscopic Method for Bilirubin Estimation in Neonates.
Journal of biophotonics Pub Date : 2025-02-20 DOI: 10.1002/jbio.202400505
Chitra R, Shanthi Prince, Ashok Chandrasekaran, Subash Sundar
{"title":"Development and Validation of a Noninvasive Diffuse Reflectance Spectroscopic Method for Bilirubin Estimation in Neonates.","authors":"Chitra R, Shanthi Prince, Ashok Chandrasekaran, Subash Sundar","doi":"10.1002/jbio.202400505","DOIUrl":"https://doi.org/10.1002/jbio.202400505","url":null,"abstract":"<p><p>Jaundice due to excess bilirubin buildup in neonates remains an alarming problem, especially during the very first week of postnatal life. The common method of measuring total serum bilirubin (TSB) levels involves invasive blood testing, which is time-consuming and uncomfortable for neonates. While noninvasive transcutaneous bilirubinometers (TcB) are available, that often lack accuracy due to interference from other biological chromophores. This study aims to improve bilirubin assessment by developing a noninvasive system that uses diffuse reflectance spectroscopy and a novel algorithm accounting for chromophores like melanin and hemoglobin. Focusing on the sternum as the measurement site, spectra are collected from 285 newborns. Using the proposed algorithm, the bilirubin concentration is calculated at 450, 460, and 470 nm. Compared to TSB levels, the proposed method demonstrated strong accuracy with an average bias of -0.10 mg/dL and narrower limits of agreement of 4.85 mg/dL at 470 nm.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400505"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143470436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.
Journal of biophotonics Pub Date : 2025-02-19 DOI: 10.1002/jbio.202400450
V I Shcheslavskiy, M V Shirmanova, K S Yashin, A C Rück, M C Skala, W Becker
{"title":"Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.","authors":"V I Shcheslavskiy, M V Shirmanova, K S Yashin, A C Rück, M C Skala, W Becker","doi":"10.1002/jbio.202400450","DOIUrl":"https://doi.org/10.1002/jbio.202400450","url":null,"abstract":"<p><p>This article gives an overview of the most frequently used fluorescence-lifetime imaging (FLIM) techniques, their capabilities, and typical applications. Starting from a general introduction to fluorescence and phosphorescence lifetime, we will show that the fluorescence lifetime or, more accurately, the fluorescence decay function of a fluorophore is a direct indicator of the interaction with its molecular environment. FLIM is therefore more than a simple contrast technique in microscopy-it is a technique of molecular imaging. FLIM techniques can be classified into time-domain and frequency-domain techniques, analogue and photon counting techniques, and scanning and wide-field techniques. Starting from an overview of these general technical principles we will describe the features and peculiarities of the different FLIM techniques in use. An extended section is dedicated to TCSPC FLIM, addressing unique capabilities that make the technique especially interesting to FLIM of biological systems.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400450"},"PeriodicalIF":0.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photobiomodulation of Blue Light Enhance Melanoma Cellular Recovery Following Viral Infection via Mitochondrial Pathways.
Journal of biophotonics Pub Date : 2025-02-17 DOI: 10.1002/jbio.202400525
Haokuan Qin, Hui Jiang, Shijie Huang, Jiali Yang, Qiqi Fu, Ruixiao Zhang, Zeqing Chen, Muqing Liu
{"title":"Photobiomodulation of Blue Light Enhance Melanoma Cellular Recovery Following Viral Infection via Mitochondrial Pathways.","authors":"Haokuan Qin, Hui Jiang, Shijie Huang, Jiali Yang, Qiqi Fu, Ruixiao Zhang, Zeqing Chen, Muqing Liu","doi":"10.1002/jbio.202400525","DOIUrl":"https://doi.org/10.1002/jbio.202400525","url":null,"abstract":"<p><p>This study aimed to elucidate the response of B16F10 cells to blue light following infection with an empty adenoviral vector (Adv). Twenty-four hours post-infection, the B16F10 cells underwent phototherapy, and a range of cellular parameters were assessed, including cell viability, melanin content, cell cycle progression, reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP), apoptosis, necrosis, lactate dehydrogenase (LDH) release, and autophagy. The findings indicated that Adv infection elevated melanin content and autophagy, resulting in G1 phase cell cycle arrest. In comparison to the control group, light-treated, Adv-infected cells exhibited reduced inhibition rates, LDH release, apoptosis, and necrosis, while MMP and ROS levels were elevated. The study concluded that although Adv, as a gene delivery vector in conjunction with light therapy, significantly modulated melanin content and autophagy. Moreover, while melanin's light absorption capacity mitigated MMP reduction, the resultant excitation molecules generated ROS, enhancing oxidative stress.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400525"},"PeriodicalIF":0.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing UVA and Laser-Induced Crosslinking via Brillouin Microscopy.
Journal of biophotonics Pub Date : 2025-02-16 DOI: 10.1002/jbio.202400401
Christian A Iriarte-Valdez, Johannes Wenzel, Emilie Baron, Alexandra Y Claus, Stefan Kalies, Karsten Sperlich, Oliver Stachs, Maria Leilani Torres-Mapa, Alexander Heisterkamp
{"title":"Assessing UVA and Laser-Induced Crosslinking via Brillouin Microscopy.","authors":"Christian A Iriarte-Valdez, Johannes Wenzel, Emilie Baron, Alexandra Y Claus, Stefan Kalies, Karsten Sperlich, Oliver Stachs, Maria Leilani Torres-Mapa, Alexander Heisterkamp","doi":"10.1002/jbio.202400401","DOIUrl":"https://doi.org/10.1002/jbio.202400401","url":null,"abstract":"<p><p>Keratoconus and other corneal ectatic disorders involve the degradation of collagen fibers, which compromises the corneal biomechanical properties. Ultraviolet-A (UVA) crosslinking has emerged as the primary treatment to slow down collagen degradation. This treatment is limited in both penetration depth and spatial precision, potentially leading to unwanted side effects. This study compares the changes in biomechanical properties of corneas crosslinked with UVA irradiation and a near-infrared femtosecond laser, using Brillouin microscopy. The biomechanical properties of the crosslinked regions were mapped in terms of Brillouin frequency shift in three dimensions. UVA crosslinking showed an average increase in Brillouin frequency shift of ~100 MHz. We demonstrate targeted spatial and axial corneal femtosecond crosslinking, with similar Brillouin frequency shift values to UVA in crosslinked regions.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400401"},"PeriodicalIF":0.0,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Non-Contact Heart Rate Measurement Using Ghost Imaging System.
Journal of biophotonics Pub Date : 2025-02-10 DOI: 10.1002/jbio.202400517
Jianming Yu, Yuchen He, Bin Li, Hui Chen, Huaibin Zheng, Jianbin Liu, Lifan Zhou, Yi Niu, Haodong Wu, Zhuo Xu
{"title":"Non-Contact Heart Rate Measurement Using Ghost Imaging System.","authors":"Jianming Yu, Yuchen He, Bin Li, Hui Chen, Huaibin Zheng, Jianbin Liu, Lifan Zhou, Yi Niu, Haodong Wu, Zhuo Xu","doi":"10.1002/jbio.202400517","DOIUrl":"https://doi.org/10.1002/jbio.202400517","url":null,"abstract":"<p><p>Remote heart rate measurement is an increasingly concerned research field, usually using remote photoplethysmography to collect heart rate information through video data collection. However, in certain specific scenarios (such as low light conditions, intense lighting, and non-line-of-sight situations), traditional methods fail to capture image information effectively, that may lead to difficulty or inability in measuring heart rate. To address these limitations, this study proposes non-contact heart rate detection based on ghost imaging architecture. The mean absolute error between experimental measurements and reference true values is 4.24 bpm. Additionally, the bucket signals obtained by the ghost imaging system can be directly processed using digital signal processing techniques, thereby enhancing personal privacy protection.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400517"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploratory Raman Spectroscopic Studies of Canine Oral Tumour Types.
Journal of biophotonics Pub Date : 2025-02-09 DOI: 10.1002/jbio.202400372
Priyanka A Jadhav, Arti Hole, Panchali Saha, Kshama Pansare, Aishwarya Ghanwat, Poonam Gera, Kiran Bendale, C Murali Krishna, Pradip Chaudhari
{"title":"Exploratory Raman Spectroscopic Studies of Canine Oral Tumour Types.","authors":"Priyanka A Jadhav, Arti Hole, Panchali Saha, Kshama Pansare, Aishwarya Ghanwat, Poonam Gera, Kiran Bendale, C Murali Krishna, Pradip Chaudhari","doi":"10.1002/jbio.202400372","DOIUrl":"https://doi.org/10.1002/jbio.202400372","url":null,"abstract":"<p><p>Canine cancers are becoming increasingly significant due to their natural occurrence, similar to the spontaneous cancers in humans as well as their histological and biological similarities to human cancers. Several oral cancer types have been witnessed in dogs, based on the cell type from which the tumour originates. The type of oral tumour dictates severity of the disease, treatment options and prognoses. The current tissue-based Raman Spectroscopy (RS) study explores stratification of canine cancers. Raman spectra of histopathologically confirmed normal and oral tumour types namely Epulis, Spindle cell sarcoma (SCS), and Squamous cell carcinoma (SCC) were acquired using a Raman confocal microscope with 532 nm laser, pre-processed and multivariate analyses performed. PC-LDA achieved overall classification accuracy of > 70%. Thus, the present study evaluates potential of RS to identify the tumour type based on the identification of characteristic spectral features. Findings warrant large scale in vivo RS explorations in canine cancer subjects.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400372"},"PeriodicalIF":0.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced Hair Regrowth Through Dual-Wavelength Low-Level Laser Therapy: A Comparative Study on Mice.
Journal of biophotonics Pub Date : 2025-02-09 DOI: 10.1002/jbio.202400523
He Gao, Yao Liu, Zhaiyi Liu, Pengquan Wang, Zhaoyin Qin, Shuanglin Liao, Jia Mo, Lin Wang, Hsiang-Chen Chui
{"title":"Enhanced Hair Regrowth Through Dual-Wavelength Low-Level Laser Therapy: A Comparative Study on Mice.","authors":"He Gao, Yao Liu, Zhaiyi Liu, Pengquan Wang, Zhaoyin Qin, Shuanglin Liao, Jia Mo, Lin Wang, Hsiang-Chen Chui","doi":"10.1002/jbio.202400523","DOIUrl":"https://doi.org/10.1002/jbio.202400523","url":null,"abstract":"<p><p>Low-level laser therapy (LLLT) is a non-invasive treatment that uses low-power lasers or light sources to stimulate biological responses. Previous studies focused on single-wavelength effects on hair growth in mice. In this study, we investigated dual-wavelength combinations to enhance hair regrowth. Using light-emitting diodes (LEDs) at 670, 680, 780, and 880 nm, we assessed the correlation between different wavelength pairs and hair regrowth after shaving in mice. Phototherapy was conducted over 14 days, with results analyzed through surface image analysis and histological examination. Our findings indicate that dual-wavelength therapy is superior to single-wavelength treatment and significantly more effective than the untreated control, demonstrating its potential for improved hair regrowth.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400523"},"PeriodicalIF":0.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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