Journal of biophotonics最新文献_第5页

Label-Free Intraoperative Diagnosis of Breast Cancer Based on Multi-Angle Orthogonal Polarization Microscopy and Multimodal Fusion. 基于多角度正交偏振显微镜和多模态融合的无标记乳腺癌术中诊断。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70259

Jianbo Zhu, Wenhui Sun, Jinjin Wu, Sheng Ding, Chongyan Liu, Zichen Ling, Linwei Shang, Huijie Wang, Jing Pei, Jianhua Yin

{"title":"Label-Free Intraoperative Diagnosis of Breast Cancer Based on Multi-Angle Orthogonal Polarization Microscopy and Multimodal Fusion.","authors":"Jianbo Zhu, Wenhui Sun, Jinjin Wu, Sheng Ding, Chongyan Liu, Zichen Ling, Linwei Shang, Huijie Wang, Jing Pei, Jianhua Yin","doi":"10.1002/jbio.70259","DOIUrl":"10.1002/jbio.70259","url":null,"abstract":"Breast cancer surgery urgently requires a rapid and objective intraoperative diagnostic strategy, as conventional frozen-section pathology depends on time-consuming staining and subjective interpretation, limiting timely surgical decision-making. However, existing label-free microscopic imaging approaches exclude conventional pathological information and lack diagnostic power. To overcome these limitations, a multimodal fusion framework based on unstained multi-angle orthogonal polarization micro-imaging (OPMI) and bright-field micro-imaging (BFMI) was proposed for rapid intraoperative breast cancer diagnosis. By superimposing and differencing multi-angle polarized images, optical contrast of anisotropic collagen fibers was markedly enhanced without staining, enabling objective visualization of collagen morphology and spatial alterations associated with malignancy. Local radial alignment and increased density of collagen fibers corresponding to invasive progression were identified. A dual-encoder fusion network integrating global and local feature representation improved lesion structure and boundary delineation. The method achieved 91.83% accuracy and area under the curve (AUC) of 0.9295, outperforming unimodal and bimodal approaches, offering a promising label-free intraoperative decision-support strategy.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70259"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147517919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical Molecular Signatures of IDH Genotype for Classification of Human Glioma Using Visible Resonance Raman Spectroscopy. IDH基因型的光学分子特征用于人类胶质瘤的可见共振拉曼光谱分类

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70239

Liang Zhang, Yan Zhou, Binlin Wu, Shengjia Zhang, Ke Zhu, Cheng-Hui Liu, Robert Alfano, Xinguang Yu

引用次数: 0

LED Photobiomodulation for Pain Reduction in Temporomandibular Disorder: A Randomized, Controlled, and Blinded Clinical Trial. LED光生物调节减轻颞下颌紊乱的疼痛：一项随机、对照和盲法临床试验。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70243

Lucía Píriz Trindade, Maria Jose Urruty, Laura Hermida Bruno, Federico Todeschini, Luis Eduardo Pascuali Moya, Valentina Lestido, Tainá Caroline Dos Santos Malavazzi, Lara Jansiski Motta, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes, Anna Carolina Ratto Tempestini Horliana, Raquel Agnelli Mesquita-Ferrari

{"title":"LED Photobiomodulation for Pain Reduction in Temporomandibular Disorder: A Randomized, Controlled, and Blinded Clinical Trial.","authors":"Lucía Píriz Trindade, Maria Jose Urruty, Laura Hermida Bruno, Federico Todeschini, Luis Eduardo Pascuali Moya, Valentina Lestido, Tainá Caroline Dos Santos Malavazzi, Lara Jansiski Motta, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes, Anna Carolina Ratto Tempestini Horliana, Raquel Agnelli Mesquita-Ferrari","doi":"10.1002/jbio.70243","DOIUrl":"10.1002/jbio.70243","url":null,"abstract":"This clinical, randomized, controlled, and blinded trial evaluated photobiomodulation (PBM) using 850 nm (infrared) and 660 nm (red) LED clusters on cervical and mandibular mobility and masticatory muscles in temporomandibular disorder (TMD) patients. PBM was applied to the temporomandibular joint (TMJ) region, masseter, temporal, scalene, and trapezius muscles over six non-consecutive sessions over 2 weeks. The Research Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) were used to determine the presence of TMD. A caliper and goniometer were used to measure the mandibular and cervical range of motion, respectively. Pain was assessed with the visual analog scale (VAS) before and after intervention. Results showed pain reduction only in the LED group post-intervention; the placebo group showed no difference. No differences were observed in mouth opening, right laterality, flexion, or extension between groups, but left laterality increased in the LED group. In conclusion, PBM with LEDs effectively reduced pain in TMD patients. Trial Registration: ClinicalTrials.gov identifier: NCT06068959.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70243"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12966631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147370838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective Laser Ablation of Malignant Solid Tumors Tuned to the Resonant Wavelength of Collagen. 调谐到胶原蛋白共振波长的恶性实体瘤的选择性激光消融。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70250

Xing Huang, Dunxiang Zhang, Ning Xia, Jinmiao Guo, Ang Deng, Xuemei Yang, Han Wu, Bole Tian, Junjie Xiong, Houkun Liang

{"title":"Selective Laser Ablation of Malignant Solid Tumors Tuned to the Resonant Wavelength of Collagen.","authors":"Xing Huang, Dunxiang Zhang, Ning Xia, Jinmiao Guo, Ang Deng, Xuemei Yang, Han Wu, Bole Tian, Junjie Xiong, Houkun Liang","doi":"10.1002/jbio.70250","DOIUrl":"10.1002/jbio.70250","url":null,"abstract":"Laser ablation is progressively introducing novel therapeutic approaches for treatment of solid tumors. Recently, our previous research demonstrated that mid-infrared femtosecond lasers tuned to the collagen resonance wavelength can selectively ablate solid tumors. However, the mechanisms underlying selectivity, as well as its potential clinical relevance, remain insufficiently understood. In this study, systematic investigations have been conducted on three orthotopic mice models of solid tumors: glioma, pancreatic ductal adenocarcinoma (PDAC), and colorectal cancer, employing a 6.1 μm femtosecond laser resonant with collagen. Selective ablation is observed in glioma and PDAC, but not in colorectal cancer, which is attributed to the markedly increased collagen content, particularly Collagen I, relative to their corresponding normal tissues, a disparity that is not present in colorectal cancer. Further analysis of clinical specimens through multiplex immunofluorescence and gene expression supports the translational potential of this collagen-mediated selective laser ablation mechanism, suggesting its applicability for tumor-targeted therapeutic interventions.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70250"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147438466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated LC-OCT/Raman Microspectroscopy: A Novel Diagnostic Approach in Ophthalmology Demonstrated in a Case Study. 集成LC-OCT/拉曼显微光谱学：一种新的眼科诊断方法。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70245

Ayadh Meriem, Dorado Cortez Christian, Ogien Jonas, Vanden-Bossche Arnaud, Camy Florian, Cinotti Elisa, Grivet Damien, Dubois Arnaud, Perrot Jean-Luc

引用次数: 0

Infrared Photobiomodulation Modulates PpIX Production After Topical Methyl Aminolevulinate in Normal Human Skin: An Exploratory Study. 红外线光生物调节在正常人体皮肤局部使用甲基氨基乙酰丙酸后PpIX的产生：一项探索性研究。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70262

Letícia Palombo Martinelli, Natasha Ferreira Mezzacappo, Ana Paula Silva, Priscila Menezes, Vanderlei Salvador Bagnato

{"title":"Infrared Photobiomodulation Modulates PpIX Production After Topical Methyl Aminolevulinate in Normal Human Skin: An Exploratory Study.","authors":"Letícia Palombo Martinelli, Natasha Ferreira Mezzacappo, Ana Paula Silva, Priscila Menezes, Vanderlei Salvador Bagnato","doi":"10.1002/jbio.70262","DOIUrl":"10.1002/jbio.70262","url":null,"abstract":"Photodynamic therapy (PDT) for skin lesions requires optimized photosensitizer accumulation. Since protoporphyrin IX (PpIX) synthesis is linked to mitochondrial activity, photobiomodulation (PBM) may modulate this process. This exploratory study examined the effects of PBM at 780 or 808 nm on MAL-induced PpIX in normal human skin. Eleven volunteers received paired-arm treatments (5, 10, or 15 J/cm2). Following 30 min of MAL, PBM was applied, with total incubation lasting 210 min. PpIX was assessed via spectroscopy and widefield imaging. Although mean PBM/no-PBM ratios were nominally above 1, no statistically significant differences were found among fluences or against unity, primarily due to high interindividual variability (CV up to 35%). These findings suggest that while PBM shows a trend toward modulating photosensitizer accumulation, the response is highly heterogeneous. This study provides a rationale for further investigation into personalized PBM-assisted PDT protocols to manage biological variability and refine clinical outcomes.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70262"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13022744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147523217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation on Effects of Process Parameters on Tissue Fusion Performance and Thermal Damage in Laser-Assisted Vascular Anastomosis. 工艺参数对激光辅助血管吻合组织融合性能及热损伤影响的研究。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 Epub Date: 2025-12-19 DOI: 10.1002/jbio.202500515

Jun Huang, Mingkun Luo, Letian Wang, Hao Cui, Yiru Xia, JingJing Nan

引用次数: 0

Leveraging Laser-Induced Breakdown Spectroscopy and Machine Learning Methods for Rapid Detection of AMR Profiles in Pathogenic Bacterial Isolates. 利用激光诱导击穿光谱和机器学习方法快速检测病原菌分离物的AMR谱。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70249

Vivek Sivakumar, Sujatha N Unni, Nilesh J Vasa

{"title":"Leveraging Laser-Induced Breakdown Spectroscopy and Machine Learning Methods for Rapid Detection of AMR Profiles in Pathogenic Bacterial Isolates.","authors":"Vivek Sivakumar, Sujatha N Unni, Nilesh J Vasa","doi":"10.1002/jbio.70249","DOIUrl":"10.1002/jbio.70249","url":null,"abstract":"Rapid antimicrobial resistance (AMR) detection is critical for clinical treatment efficacy and infection control. We present a Laser-Induced Breakdown Spectroscopy (LIBS) method for antimicrobial susceptibility testing and detection of various resistance profiles in pathogenic bacteria. A controlled growth environment is utilized for regulated culture of different bacterial strains which induces strain-specific adaptation under controlled nutrient stress and also minimizes spectral variability due to differences in media composition and bacterial growth phases. By leveraging minimal yet robust spectral features (emission lines from Sodium, Potassium and Calcium), this method enables accurate AMR detection by employing machine learning algorithms. An accuracy of 94.7% and ROC-AUC > 0.99 was achieved using a Support Vector Machine model for classifying seven bacterial strains (3 susceptible and 4 resistant), without requiring outlier filtering or averaging of spectra. This tailored LIBS framework establishes a quick and cost-effective diagnostic tool for rapid AMR profiling compared to conventional culture-based susceptibility testing.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70249"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147370787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep Learning-Based Super-Resolution for Vessel Enhancement in Photoacoustic Microscopy Imaging. 基于深度学习的超分辨率光声显微镜血管增强技术。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70221

Thi Thu Ha Vu, Soonhyuk Tak, Tan Hung Vo, Van Bang Nguyen, Dinh Tuan Nguyen, Le Khuong Phan, Jaeyeop Choi, Sudip Mondal, Junghwan Oh

{"title":"Deep Learning-Based Super-Resolution for Vessel Enhancement in Photoacoustic Microscopy Imaging.","authors":"Thi Thu Ha Vu, Soonhyuk Tak, Tan Hung Vo, Van Bang Nguyen, Dinh Tuan Nguyen, Le Khuong Phan, Jaeyeop Choi, Sudip Mondal, Junghwan Oh","doi":"10.1002/jbio.70221","DOIUrl":"10.1002/jbio.70221","url":null,"abstract":"Photoacoustic imaging (PAI) is an advanced imaging technique for high-resolution (HR), non-invasive visualization of vascular networks, offering distinct advantages in functional and structural imaging. However, its performance is often constrained by trade-offs between spatial resolution and imaging depth, as well as noise and artifacts caused by system limitations and tissue properties. Here, we introduce GDSU-Net, a fine-tuned neural network designed for super-resolution (SR) reconstruction of PAI. GDSU-Net builds on the U-Net architecture and incorporates four key components: group normalization, depthwise separable convolutions, squeeze-and-excitation (SE) blocks, and a pixelshuffle-based decoder. Experimental results demonstrate that GDSU-Net achieves a structural similarity index of 0.889 and a peak signal-to-noise ratio (PSNR) of 31.979 dB, while reducing the root mean square error (RMSE) to 0.032 and the mean absolute error (MAE) to 0.025. Visual evaluations confirm its effectiveness in restoring vascular details with preserved anatomical fidelity. These findings highlight GDSU-Net as a computationally efficient solution for super-resolution enhancement in PAI.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70221"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147358196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Situ 3D Super Resolution Nanoscale Imaging of Cell Spheroids by Expansion Microscopy. 细胞球体的原位三维超分辨率纳米级扩增显微镜成像。

IF 2.3

Journal of biophotonics Pub Date : 2026-03-01 DOI: 10.1002/jbio.70253

Junfang Xie, Yifei Zhan, Jin Wang, Zhichao Deng, Jian-Guo Tian, Qing Ye

引用次数: 0