Journal of biophotonics最新文献_第5页

The Quantitative In Vivo Assessment of Diabetic and Non-Diabetic Skin Wound Healing Using Phasor-FLIM Approach. 利用 Phasor-FLIM 方法对糖尿病和非糖尿病皮肤伤口愈合进行体内定量评估

Journal of biophotonics Pub Date : 2024-10-29 DOI: 10.1002/jbio.202400227

Hala Zuhayri, Tatiana B Lepekhina, Viktor V Nikolaev, Houssain Baalbaki, Anastasia I Knyazkova, Alexey V Borisov, Natalya A Krivova, Yury V Kistenev

{"title":"The Quantitative In Vivo Assessment of Diabetic and Non-Diabetic Skin Wound Healing Using Phasor-FLIM Approach.","authors":"Hala Zuhayri, Tatiana B Lepekhina, Viktor V Nikolaev, Houssain Baalbaki, Anastasia I Knyazkova, Alexey V Borisov, Natalya A Krivova, Yury V Kistenev","doi":"10.1002/jbio.202400227","DOIUrl":"https://doi.org/10.1002/jbio.202400227","url":null,"abstract":"A quantitative assessment of wound status in a murine model was developed using phasor plot presentation of fluorescence lifetime imaging microscopy (FLIM) data. The quantitative assessment is based on calculating Bhattacharyya distance between g coordinates of FLIM data phasor plot density distributions of wound and healthy skin. The approach was validated for both diabetic and non-diabetic mice wounds, including during low-dose photodynamic therapy (LDPDT). Analysis revealed a shift in the FLIM data phasor plot g coordinates, suggesting altered metabolic processes involved in wound healing. Bhattacharyya distances in the LDPDT groups were closer to zero compared to the control group, which was not treated by LDPDT. Bhattacharyya distances in the non-diabetic LDPDT groups were closer to zero compared to the diabetic LDPDT groups that is consistent with the literature regarding the positive role of LDPDT in accelerating wound healing and the role of diabetes mellitus in impairing wound healing.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400227"},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tutorial on the Use of the Photon Diffusion Approximation for Fast Calculation of Tissue Optical Properties. 关于使用光子扩散近似法快速计算组织光学特性的教程。

Journal of biophotonics Pub Date : 2024-10-26 DOI: 10.1002/jbio.202400257

Maria R Pinheiro, Maria I Carvalho, Luís M Oliveira

引用次数: 0

Investigation of Changes in the Reflection Spectrum of Human Blood When Exposed to Laser Radiation With Wavelengths of 450 or 980 nm. 波长为 450 或 980 纳米的激光照射下人体血液反射光谱变化的研究。

Journal of biophotonics Pub Date : 2024-10-20 DOI: 10.1002/jbio.202400251

V Yu Chuchin, A A Masharskaya, A V Belikov

引用次数: 0

Raman Microspectroscopy of Hair: Low-Frequency Markers of Protein Secondary Structure. 头发的拉曼微光谱学：蛋白质二级结构的低频标记。

Journal of biophotonics Pub Date : 2024-10-13 DOI: 10.1002/jbio.202400265

E I Travkina, A Yu Chikishev, N N Brandt

引用次数: 0

Novel Diagnostic Approach for Acute Pharyngitis: Combining Machine Learning With Thermal Imaging. 急性咽炎的新型诊断方法：将机器学习与热成像技术相结合。

Journal of biophotonics Pub Date : 2024-10-13 DOI: 10.1002/jbio.202400219

Oshrit Hoffer, Moriya Cohen, Maya Gerstein, Vered Shkalim Zemer, Yael Reichenberg, Dima Bykhovsky, Moshe Hoshen, Herman Avner Cohen

引用次数: 0

High-Resolution Terahertz Spectroscopy for Medical Diagnostics. 用于医学诊断的高分辨率太赫兹光谱学。

Journal of biophotonics Pub Date : 2024-10-12 DOI: 10.1002/jbio.202400316

Vladimir Vaks, Elena Domracheva, Maria Chernyaeva, Vladimir Anfertev, Andrey Ayzenshtadt, Kseniya Glushkova, Aleksandra Cherniaeva

{"title":"High-Resolution Terahertz Spectroscopy for Medical Diagnostics.","authors":"Vladimir Vaks, Elena Domracheva, Maria Chernyaeva, Vladimir Anfertev, Andrey Ayzenshtadt, Kseniya Glushkova, Aleksandra Cherniaeva","doi":"10.1002/jbio.202400316","DOIUrl":"https://doi.org/10.1002/jbio.202400316","url":null,"abstract":"The metabolomics-based approach to diagnostics and therapy monitoring is a fast-emerging trend in modern medicine. Terahertz nonstationary spectroscopy based on the induction and disintegration of freely decaying polarization in the gas mixture during the interaction of radiation with molecules at resonance frequencies is a high-sensitivity method for studying multicomponent gas mixtures, which is promising for identifying metabolites in the thermal decomposition products of biological samples. The paper presents the results of the application of high-resolution terahertz spectroscopy to the study of biological samples taken from patients with certain diseases (pathologically changed tissues of ear-nose-throat organs and similar pathologic tissues formed in other life support systems) to search for characteristic sets of metabolites characterizing the pathology. The world's first measurements of the spectra of pathologic samples of cysts, formed in different life support systems, were carried out, which made it possible to identify similar substances in tissues having the same pathology.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400316"},"PeriodicalIF":0.0,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simple and Fast Optical Clearing Method for Whole-Mount Fluorescence In Situ Hybridization (FISH) Imaging. 用于整装荧光原位杂交 (FISH) 成像的简单快速光学清除方法。

Journal of biophotonics Pub Date : 2024-10-10 DOI: 10.1002/jbio.202400258

Junwoo Suh, Yehe Liu, Jordan Smith, Michiko Watanabe, Andrew M Rollins, Michael W Jenkins

{"title":"A Simple and Fast Optical Clearing Method for Whole-Mount Fluorescence In Situ Hybridization (FISH) Imaging.","authors":"Junwoo Suh, Yehe Liu, Jordan Smith, Michiko Watanabe, Andrew M Rollins, Michael W Jenkins","doi":"10.1002/jbio.202400258","DOIUrl":"10.1002/jbio.202400258","url":null,"abstract":"We report a single-step optical clearing method that is compatible with RNA fluorescence in situ hybridization (FISH) imaging. We previously demonstrated microscopy imaging with immunohistochemistry and genetic reporters using a technique called lipid-preserving refractive index matching for prolonged imaging depth (LIMPID). Our protocol reliably produces high-resolution three-dimensional (3D) images with minimal aberrations using high magnification objectives, captures large field-of-view images of whole-mount tissues, and supports co-labeling with antibody and FISH probes. We also custom-designed FISH probes for quail embryos, demonstrating the ease of fabricating probes for use with less common animal models. Furthermore, we show high-quality 3D images using a conventional fluorescence microscope, without using more advanced depth sectioning instruments such as confocal or light-sheet microscopy. For broader adoption, we simplified and optimized 3D-LIMPID-FISH to minimize the barrier to entry, and we provide a detailed protocol to aid users with navigating the thick and thin of 3D microscopy.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400258"},"PeriodicalIF":0.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Graphene Oxide Nanoparticles on In Vitro Development of a Mouse Preimplantation Embryo and Interaction With the Zona Pellucida. 氧化石墨烯纳米粒子对小鼠植入前胚胎体外发育的影响以及与透明带的相互作用

Journal of biophotonics Pub Date : 2024-10-01 DOI: 10.1002/jbio.202400268

Artashes V Karmenyan, Alexander S Krivokharchenko, Micahella N Sarmiento, Eviyona L Barus, Elena V Perevedentseva, Chia-Liang Cheng

{"title":"Impact of Graphene Oxide Nanoparticles on In Vitro Development of a Mouse Preimplantation Embryo and Interaction With the Zona Pellucida.","authors":"Artashes V Karmenyan, Alexander S Krivokharchenko, Micahella N Sarmiento, Eviyona L Barus, Elena V Perevedentseva, Chia-Liang Cheng","doi":"10.1002/jbio.202400268","DOIUrl":"https://doi.org/10.1002/jbio.202400268","url":null,"abstract":"The development of assisted reproductive technologies increases the likelihood of nanoparticles' (NPs) direct contact with gametes and embryos in in vitro conditions. Analyzing the influence of nanomaterials on the early mammalian embryo becomes increasingly relevant. This work is devoted to the effect of graphene oxide (GO) NPs on the in vitro development of mammalian embryos. Mouse 2-cell embryos were preincubated with GO NPs. The interaction of GO with the Zona Pellucida (ZP) of the embryo was investigated using fluorescence lifetime imaging with two-photon excitation (2p-FLIM). During embryo development, the NPs penetration into ZP (blastocyst stage) and perivitelline space (blastocyst hatching stage) was observed. Despite this, GO did not affect the embryo's ability to develop till late and hatching blastocysts. The mechanism of the NPs getting into the perivitelline space and the consequences of NP-embryo direct contact are discussed. The 2p-FLIM efficiency for studying NP interaction with mammalian embryos is evaluated.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400268"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoplethysmography-Based Angiography of Skin Tumors in Arbitrary Areas of Human Body. 基于光速摄影的人体任意部位皮肤肿瘤血管造影。

Journal of biophotonics Pub Date : 2024-09-26 DOI: 10.1002/jbio.202400242

Anastasia Guryleva, Alexander Machikhin, Ekaterina Orlova, Evgeniya Kulikova, Michail Volkov, Gaiane Gabrielian, Ludmila Smirnova, Marina Sekacheva, Olga Olisova, Ekaterina Rudenko, Olga Lobanova, Vera Smolyannikova, Tatiana Demura

{"title":"Photoplethysmography-Based Angiography of Skin Tumors in Arbitrary Areas of Human Body.","authors":"Anastasia Guryleva, Alexander Machikhin, Ekaterina Orlova, Evgeniya Kulikova, Michail Volkov, Gaiane Gabrielian, Ludmila Smirnova, Marina Sekacheva, Olga Olisova, Ekaterina Rudenko, Olga Lobanova, Vera Smolyannikova, Tatiana Demura","doi":"10.1002/jbio.202400242","DOIUrl":"https://doi.org/10.1002/jbio.202400242","url":null,"abstract":"Noninvasive, rapid, and robust diagnostic techniques for clinical screening of tumors located in arbitrary areas of the human body are in demand. To address this challenge, we analyzed the feasibility of photoplethysmography-based angiography for assessing vascular structures within malignant and benign tumors. The proposed hardware and software were approved in a clinical study involving 30 patients with tumors located in the legs, torso, arms, and head. High-contrast and detailed vessel maps within both benign and malignant tumors were obtained. We demonstrated that capillary maps are consistent and can be interpreted using well-established dermoscopic criteria for vascular morphology. Vessel mapping provides valuable details, which may not be available in dermoscopic images and can aid in determining whether a tumor is benign or malignant. We believe that the proposed approach may become a valuable tool in the preliminary cancer diagnosis and is suitable for large-scale screening.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400242"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microsaccades Tracking by Secondary Speckle Pattern Analysis. 通过二次斑点模式分析追踪微注视

Journal of biophotonics Pub Date : 2024-09-09 DOI: 10.1002/jbio.202400184

Ola Shteinberg, Sergey Agdarov, Yafim Beiderman, Yoram S Bonneh, Inbal Ziv, Zeev Zalevsky

引用次数: 0