Journal of biophotonicsPub Date : 2026-03-01Epub Date: 2025-12-14DOI: 10.1002/jbio.202500418
V Andreev, M Yakovleva, A Kostyukov, V Sokolova, V Shcheslavskiy, G Goltsman, T Feldman, V Kuzmin, M Ostrovsky, Pavel Morozov
{"title":"Interrogation of Retinal Lipofuscin by Fluorescence Lifetime Imaging Microscopy.","authors":"V Andreev, M Yakovleva, A Kostyukov, V Sokolova, V Shcheslavskiy, G Goltsman, T Feldman, V Kuzmin, M Ostrovsky, Pavel Morozov","doi":"10.1002/jbio.202500418","DOIUrl":"10.1002/jbio.202500418","url":null,"abstract":"<p><p>Age-related macular degeneration is a disease that affects the middle part of the vision and involves pathological alterations in the retinal pigment epithelium. Accurate and timely evaluation of the retinal pigment epithelium is a cornerstone of effective treatment planning. In this study, we present the development of a preclinical method for early diagnostics of age-related macular degeneration using time and spectral characteristics of fluorescence of lipofuscin granules from the retinal pigment epithelium. Using the unique system based on a superconducting single-photon detector and time-correlated single-photon counting electronics integrated in the confocal laser scanning microscope we determined the parameters of fluorescence (distribution long and short fluorescence lifetime components and their contribution to the total fluorescence signal as well as fluorescence spectral shift) that have a diagnostic value for differentiation of the normal and pathological states in the degenerative diseases of the retina and retinal pigment epithelium.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500418"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145759085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Monitoring of Tissue Perfusion During Arterial Occlusion With Remote Photoplethysmographic Imaging.","authors":"Leah DeVos, Gennadi Saiko, Vanja Dvekar, Alexandre Douplik","doi":"10.1002/jbio.70247","DOIUrl":"10.1002/jbio.70247","url":null,"abstract":"<p><strong>Background: </strong>Arterial occlusion is commonly used clinically, yet no standardized protocol exists for cuff pressure or placement. Different pressures and locations may elicit varying physiological responses. This study investigates whether local and systemic reactions vary with pressure and cuff location.</p><p><strong>Methods: </strong>Healthy volunteers underwent arterial occlusion at the wrist or upper arm using a cuff inflated to 150 or 200 mmHg. Remote photoplethysmography (rPPG) was used to monitor the occluded and control hands. We analyzed AC components (heart rate, respiratory rate, low-frequency activity) and signal amplitude changes in red and green channels during occlusion and reperfusion.</p><p><strong>Results: </strong>No significant differences were observed in local responses between 150 and 200 mmHg occlusions or between cuff positions.</p><p><strong>Conclusions: </strong>These findings establish 150 mmHg cuff pressure on the lower arm as a suitable arterial occlusion protocol and support rPPG for detecting perfusion changes. Minor systemic differences were noted compared to the control hand.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70247"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13022742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147523288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mehmood Pirzada, Sachini H Ekanayake, Avash Kattel, Supriya Dhakal, Hemendra Ghimire, Michael Nelson, Mathes K Dayananda, Didier Merlin, A G Unil Perera
{"title":"Monitoring Biomolecular Changes During Colitis Progression Using Infrared Spectroscopy in Dextran Sodium Sulfate-Treated Mouse Serum.","authors":"Mehmood Pirzada, Sachini H Ekanayake, Avash Kattel, Supriya Dhakal, Hemendra Ghimire, Michael Nelson, Mathes K Dayananda, Didier Merlin, A G Unil Perera","doi":"10.1002/jbio.70235","DOIUrl":"10.1002/jbio.70235","url":null,"abstract":"<p><p>Analysis of sequential alterations in body fluid constituents, such as serum, is vital for assessing inflammation. Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy was used to monitor biomolecular changes in a Dextran Sodium Sulfate (DSS) mouse colitis model. Serum samples collected on days 0, 3, and 7 post- (3% DSS) administration showed stepwise changes in lipid, protein, and carbohydrate regions. Statistically significant (SS) changes (p < 0.05) were detected at the initial stage of colitis (day 0 to 3) in six spectral biomarkers (SBMs), including the integral ratio of α-helix to β-sheet (Amide I) and the β-sheet (Amide I) to Tyrosine (Amide II). Changes intensified from day 3 to 7, with the most prominent differences from day 0 to 7, even when the initial changes (day 0 to 3) were not SS. These results highlight the potential of ATR-FTIR as a minimally invasive pre-screening tool for monitoring colitis progression.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70235"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147346105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joël Le Galudec, Mathieu Dupoy, Boris Taurel, Joris Baraillon, Pierre R Marcoux, Laurent Duraffourg
{"title":"Multispectral Infrared Colony Phenotyping for High-Throughput Microbiological Control of Waters.","authors":"Joël Le Galudec, Mathieu Dupoy, Boris Taurel, Joris Baraillon, Pierre R Marcoux, Laurent Duraffourg","doi":"10.1002/jbio.70252","DOIUrl":"10.1002/jbio.70252","url":null,"abstract":"<p><p>Microbiological water quality assessment relies on culture-based methods that are time-consuming, resource-intensive, and often lack specificity. To address these limitations, we developed a prototype for automated, label-free, and nondestructive microbial identification based on discrete frequency infrared (DFIR) multispectral imaging. By combining monochromatic quantum cascade lasers (QCLs) with an uncooled bolometer array, this prototype captures spectral and morphological fingerprints of colonies directly on filtration membranes. A demonstration database of 3230 colonies from 11 strains across 7 genera was acquired. In average, deep-learning based classification achieved a 96.5% ± 1.3% correct identification rate. Overall, this prototype brings DFIR imaging one step closer to an industry-ready microbial identification tool.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70252"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12987706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147461573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linjing Shi, Liwen Hu, Linghan You, Xiaoli Su, Shuoyu Xu, Rong Chen, Xingfu Wang, Jianxin Chen, Na Fang
{"title":"Label-Free Identification of Choroidal Melanoma Infiltration Boundaries Using Multiphoton Microscopy Combined With Image Analysis.","authors":"Linjing Shi, Liwen Hu, Linghan You, Xiaoli Su, Shuoyu Xu, Rong Chen, Xingfu Wang, Jianxin Chen, Na Fang","doi":"10.1002/jbio.70241","DOIUrl":"10.1002/jbio.70241","url":null,"abstract":"<p><p>Currently, local excision of choroidal melanoma primarily depends on the intraoperative visual assessment. To improve surgical precision, we employed multiphoton microscopy (MPM) to characterize the microstructural and spectral features of both normal tissue and tumor boundary. The spectral differences between normal choroid and tumor boundary were systematically analyzed using lambda mode MPM. Collagen parameters were quantified by combining with image processing techniques to elucidate the structural alterations of normal tissues and choroidal melanomas. The results of the study demonstrated that the MPM enables label-free identification of tumor boundary, with NADH molecular changes serving as a critical basis for qualitative differentiation. Furthermore, utilizing self-developed image processing algorithms, the alteration of collagen fiber provides a potential quantitative index for identifying the tumor boundary. This study confirms the capability of MPM in identifying tumor boundary. With the advancement of multiphoton endoscopes, it holds promise for providing precise intraoperative guidance during choroidal melanoma resection.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":"19 3","pages":"e70241"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147438804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of biophotonicsPub Date : 2026-03-01Epub Date: 2025-12-15DOI: 10.1002/jbio.202500422
Julia Grundmann, Christian Hannig, Svea Steuer, Tobias Rosenauer, Lars Kirsten, Edmund Koch, Jonas Golde, Julia Walther
{"title":"Depolarization-Based Multimodal Optical Imaging of Carious Lesions.","authors":"Julia Grundmann, Christian Hannig, Svea Steuer, Tobias Rosenauer, Lars Kirsten, Edmund Koch, Jonas Golde, Julia Walther","doi":"10.1002/jbio.202500422","DOIUrl":"10.1002/jbio.202500422","url":null,"abstract":"<p><p>A comprehensive image catalog from an in vitro investigation of teeth with occlusal carious lesions is provided. The aim was to visualize carious lesions using various imaging techniques in order to present different stages of caries progression and to provide reference data for the development and validation of polarization-sensitive optical coherence tomography (PS-OCT) as a non-ionizing diagnostic method for dental caries. The study covers a variety of approaches, bridging the gap from basic histopathological analysis of thin sections to future optical imaging of occlusal carious lesions in vivo. All measurements were performed to support the interpretation and validation of PS-OCT imaging based on the degree of polarization (DOP).</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500422"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12980561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145764863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of biophotonicsPub Date : 2026-03-01Epub Date: 2025-12-14DOI: 10.1002/jbio.202500380
Adam Wax, Haoran Zhang, Evan T Jelly, Hillel B Price, Tengfei Sun, Kengyeh K Chu, Cary C Cotton, Swathi Eluri, John R Goldblum, Nicholas J Shaheen
{"title":"Prospective Identification of Dysplasia in Barrett's Esophagus With Combined Optical Coherence Tomography and Light Scattering Measurements.","authors":"Adam Wax, Haoran Zhang, Evan T Jelly, Hillel B Price, Tengfei Sun, Kengyeh K Chu, Cary C Cotton, Swathi Eluri, John R Goldblum, Nicholas J Shaheen","doi":"10.1002/jbio.202500380","DOIUrl":"10.1002/jbio.202500380","url":null,"abstract":"<p><p>Accurate identification of dysplasia in Barrett's esophagus (BE) remains a challenge. Advanced optical imaging techniques may allow for better localization of dysplasia in BE. Here, we have assessed the potential clinical utility of a previously described multimodal imaging probe combining optical coherence tomography (OCT) with angle-resolved low coherence interferometry (a/LCI) to prospectively identify dysplasia in BE. Imaging was conducted on 37 patients undergoing endoscopic surveillance of BE, yielding co-registered biopsies of 50 esophageal sites. The a/LCI nuclear morphology data were compared to a previous decision line to prospectively predict dysplasia, demonstrating 100% sensitivity, 93% specificity, and 94% overall accuracy. The NPV was 100%, comparable to previous a/LCI studies. The addition of OCT imaging markedly improved PPV and specificity, compared to previous studies with a/LCI alone, illustrating the clinical utility of the combined platform. These findings suggest that combining OCT and a/LCI enables better detection of dysplasia by providing better guidance.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500380"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12880523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145759092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}