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Variation of Structures and Blood Vessels of Visual Nervous System With Age. 视觉神经系统结构及血管随年龄的变化。
Journal of biophotonics Pub Date : 2025-03-18 DOI: 10.1002/jbio.70009
Ni He, Chenrui Li, Jiali Liu, Xiaolong Zhang, Liming Li, Cuixia Dai
{"title":"Variation of Structures and Blood Vessels of Visual Nervous System With Age.","authors":"Ni He, Chenrui Li, Jiali Liu, Xiaolong Zhang, Liming Li, Cuixia Dai","doi":"10.1002/jbio.70009","DOIUrl":"https://doi.org/10.1002/jbio.70009","url":null,"abstract":"<p><p>Aging is a process of progressive functional decline associated with increasing age. The process and mechanism of aging have long been widely concerned, but long-term in vivo evaluations of the visual nervous system have not been previously reported. In this study, naturally aging mouse models were used for long-term serial evaluation, and the changes in structure and blood flow of the retina and cerebral cortex were systematically analyzed. Optical Coherence Tomography (OCT) and Optical Coherence Tomography Angiography (OCTA) were performed on mice at 3, 6, 9, and 12 months of age, respectively. Structural and vascular changes with age were quantitatively evaluated. Results show that the reduction of structural thickness and vascular density of the retinal and cerebral cortex is observed. A significant correlation is also found between structural and vascular changes in the retina and cerebral cortex, indicating a consistent impact of aging on the visual nervous system.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70009"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Method for Selecting the Down-Sampling Factor of Photoacoustic Image by Using Cumulative Power Difference in Frequency Domain. 利用频域累积功率差选择光声图像下采样因子的方法。
Journal of biophotonics Pub Date : 2025-03-18 DOI: 10.1002/jbio.70013
Shihao Tang, Min Wan, Yameng Zhang, Jiani Li, Ling Tao, Weitao Li
{"title":"Method for Selecting the Down-Sampling Factor of Photoacoustic Image by Using Cumulative Power Difference in Frequency Domain.","authors":"Shihao Tang, Min Wan, Yameng Zhang, Jiani Li, Ling Tao, Weitao Li","doi":"10.1002/jbio.70013","DOIUrl":"https://doi.org/10.1002/jbio.70013","url":null,"abstract":"<p><p>As a novel non-invasive imaging technology, a constraint of photoacoustic microscopy (PAM) is its imaging speed. Often, PAM utilizes sparse spatial sampling, which necessitates extensive prior experimentation to accurately select the down-sampling factors. To overcome this limitation, this study proposes a frequency-domain evaluation index, cumulative power difference (CPD), for rapid selection of the optimal down-sampling factor. We apply the proposed CPD to photoacoustic images of the ear and brain of the mouse. The result shows that as the down-sampling factor increases, there is a similar decreasing trend in the quality of the 20 images. CPD was significantly correlated with PCC/MSE/SSIM (p < 0.001). The findings suggest that CPD, with its ability to evaluate the quality of photoacoustic images and quickly quantify the quality loss of down-sampled images without prior inspection. This study contributes to expanding the application range of PAM and supporting its clinical prospects.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70013"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ElectroFluor Voltage-Sensitive Dyes: Comprehensive Analysis of Wavelength-Dependent Sensitivity and Cross-Channel Bleed-Through. 电荧光电压敏感染料：波长依赖性灵敏度和跨通道透光的综合分析。
Journal of biophotonics Pub Date : 2025-03-18 DOI: 10.1002/jbio.70008
Srdjan D Antic, Ping Yan, Corey D Acker, Olivia T Spagnola, Zehra Y Erol, Ozge Baser, Leslie M Loew
{"title":"ElectroFluor Voltage-Sensitive Dyes: Comprehensive Analysis of Wavelength-Dependent Sensitivity and Cross-Channel Bleed-Through.","authors":"Srdjan D Antic, Ping Yan, Corey D Acker, Olivia T Spagnola, Zehra Y Erol, Ozge Baser, Leslie M Loew","doi":"10.1002/jbio.70008","DOIUrl":"10.1002/jbio.70008","url":null,"abstract":"<p><p>New voltage-sensitive ElectroFluor (EF) dyes that emit across the visible and near-infrared spectrum (e.g., 730 nm) were recently developed. We evaluated EF-530, EF-630, and EF-730p-dyes spectrally orthogonal to green fluorescent protein (GFP)-at excitation wavelengths outside the conventional 470 nm range used for GFP-based indicators. Although previously applied in cardiac voltage imaging, their performance in neuronal tissue remains untested. We performed side-by-side comparisons using population voltage imaging in mouse cerebral cortex slices at optimal excitation wavelengths (530, 630, and 730 nm) and assessed cross-channel signal bleed-through across four excitation wavelengths (475, 530, 630, and 730 nm). All dyes produced robust optical signals at their optimal wavelengths, though non-preferred channels exhibited bleed-through with distinct amplitudes, polarities, and photobleaching patterns. These results provide detailed quantifications of EF dye performance for neuronal population imaging.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70008"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12284816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optomechanical Properties of Swine Skin Tissue Treated With a Nontoxic Optical Clearing Agent. 无毒光清除剂处理猪皮组织的光力学性能。
Journal of biophotonics Pub Date : 2025-03-13 DOI: 10.1002/jbio.70007
Noemi Correa, Minji Suh, Abdul Mohizin, Jinu Sudhakaran, Kee Sung Lee, Jung Kyung Kim
{"title":"Optomechanical Properties of Swine Skin Tissue Treated With a Nontoxic Optical Clearing Agent.","authors":"Noemi Correa, Minji Suh, Abdul Mohizin, Jinu Sudhakaran, Kee Sung Lee, Jung Kyung Kim","doi":"10.1002/jbio.70007","DOIUrl":"https://doi.org/10.1002/jbio.70007","url":null,"abstract":"<p><p>This study evaluated the optomechanical and structural properties of individual macroscopic layers in swine skin tissues treated with a nontoxic optical clearing agent. The clearing agent was prepared by dissolving 2,2'-thiodiethanol in a phosphate-buffered solution and applied for up to 6 days. Prolonged clearing increased both the total and unscattered transmittance. Peaks associated with deoxygenated hemoglobin, oxygenated hemoglobin, and water showed marked reduction with extended clearing times. Histopathological examination revealed no significant structural alterations. A clearing time of approximately 4-5 days is recommended for optimal imaging, as it minimizes changes in mechanical properties. These findings support the use of optical clearing in deep tissue imaging, particularly when preserving macroscopic mechanical properties is essential, despite the inherent limitation of sample opacity.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e70007"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance. 荧光寿命成像技术的原理、应用及临床意义综述
Journal of biophotonics Pub Date : 2025-02-19 DOI: 10.1002/jbio.202400450
V I Shcheslavskiy, M V Shirmanova, K S Yashin, A C Rück, M C Skala, W Becker
{"title":"Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.","authors":"V I Shcheslavskiy, M V Shirmanova, K S Yashin, A C Rück, M C Skala, W Becker","doi":"10.1002/jbio.202400450","DOIUrl":"https://doi.org/10.1002/jbio.202400450","url":null,"abstract":"<p><p>This article gives an overview of the most frequently used fluorescence-lifetime imaging (FLIM) techniques, their capabilities, and typical applications. Starting from a general introduction to fluorescence and phosphorescence lifetime, we will show that the fluorescence lifetime or, more accurately, the fluorescence decay function of a fluorophore is a direct indicator of the interaction with its molecular environment. FLIM is therefore more than a simple contrast technique in microscopy-it is a technique of molecular imaging. FLIM techniques can be classified into time-domain and frequency-domain techniques, analogue and photon counting techniques, and scanning and wide-field techniques. Starting from an overview of these general technical principles we will describe the features and peculiarities of the different FLIM techniques in use. An extended section is dedicated to TCSPC FLIM, addressing unique capabilities that make the technique especially interesting to FLIM of biological systems.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400450"},"PeriodicalIF":0.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Digital Cytometry: Extraction of Forward and Side Scattering Signals From Holotomography. 数字细胞测量：从 Holotomography 提取正向和侧向散射信号。
Journal of biophotonics Pub Date : 2025-02-05 DOI: 10.1002/jbio.202400387
Jaepil Jo, Herve Hugonnet, Mahn Jae Lee, YongKeun Park
{"title":"Digital Cytometry: Extraction of Forward and Side Scattering Signals From Holotomography.","authors":"Jaepil Jo, Herve Hugonnet, Mahn Jae Lee, YongKeun Park","doi":"10.1002/jbio.202400387","DOIUrl":"https://doi.org/10.1002/jbio.202400387","url":null,"abstract":"<p><p>Flow cytometry is a cornerstone technique in medical and biological research, providing crucial information about cell size and granularity through forward scatter (FSC) and side scatter (SSC) signals. Despite its widespread use, the precise relationship between these scatter signals and corresponding microscopic images remains underexplored. Here, we investigate this intrinsic relationship by utilizing scattering theory and holotomography, a three-dimensional quantitative phase imaging (QPI) technique. We demonstrate the extraction of FSC and SSC signals from individual, unlabeled cells by analyzing their three-dimensional refractive index distributions obtained through holotomography. Additionally, we introduce a method for digital windowing of SSC signals to facilitate effective segmentation and morphology-based cell type classification. Our approach bridges the gap between flow cytometry and microscopic imaging, offering a new perspective on analyzing cellular characteristics with high accuracy and without the need for labeling.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400387"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced Fluorescence Imaging of Implants Based on Polyester Copolymers in Combination With MRI. 基于聚酯共聚物的植入物与MRI联合增强荧光成像。
Journal of biophotonics Pub Date : 2025-02-03 DOI: 10.1002/jbio.202400147
Victoria V Zherdeva, Astemir R Likhov, Ulugbek A Saidvaliev, Dror Fixler, Dmitry Demin, Veronika N Volodina, Uliana A Apukhtina, Shweta Pawar, Bar Atuar, Valery V Tuchin
{"title":"Enhanced Fluorescence Imaging of Implants Based on Polyester Copolymers in Combination With MRI.","authors":"Victoria V Zherdeva, Astemir R Likhov, Ulugbek A Saidvaliev, Dror Fixler, Dmitry Demin, Veronika N Volodina, Uliana A Apukhtina, Shweta Pawar, Bar Atuar, Valery V Tuchin","doi":"10.1002/jbio.202400147","DOIUrl":"https://doi.org/10.1002/jbio.202400147","url":null,"abstract":"<p><p>Nowadays, many biodegradable materials are offered for biomedical applications, but there are only a few in vivo methods for their detection and monitoring. In this work, implants based on biodegradable polyester copolymers were labeled with indocyanine green (ICG) for fluorescence imaging in combination with tissue optical clearing (TOC) and magnetic resonance imaging (MRI). The results include in vitro degradation modeling followed by in vivo imaging of copolymer samples that were subcutaneously implanted in BALB/c mice. TOC with 70% glycerol has been demonstrated to significantly improve sample visualization. The TOC efficiency parameter Q demonstrated the variability of effects correlating with the timing of follow-up in the postimplantation period. It has been shown that nonhealing wounds, peri-implantation inflammation, or fibrosis, confirmed by MRI, affect the effectiveness of TOC in the range from Q = -30% to 70%.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400147"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diabetes Mellitus Impairs Blood-Brain Barrier Integrality and Microglial Reactivity. 糖尿病损害血脑屏障完整性和小胶质细胞反应性。
Journal of biophotonics Pub Date : 2025-01-27 DOI: 10.1002/jbio.202400482
Shaojun Liu, Jie Hao, Tingting Yu, Valery V Tuchin, Junming Li, Dongyu Li, Dan Zhu
{"title":"Diabetes Mellitus Impairs Blood-Brain Barrier Integrality and Microglial Reactivity.","authors":"Shaojun Liu, Jie Hao, Tingting Yu, Valery V Tuchin, Junming Li, Dongyu Li, Dan Zhu","doi":"10.1002/jbio.202400482","DOIUrl":"https://doi.org/10.1002/jbio.202400482","url":null,"abstract":"<p><p>Diabetes mellitus (DM), a chronic metabolic disorder that adversely affects the blood-brain barrier (BBB) and microglial function in the central nervous system (CNS), contributing to neuronal damage and neurodegenerative diseases. However, the underlying molecular mechanisms linking diabetes to BBB dysfunction and microglial dysregulation remain poorly understood. Here, we assessed the impacts of diabetes on BBB and microglial reactivity and investigated its mechanisms. We found diabetes severely disrupted the BBB integrity and microglial response to vascular injury. We also revealed a potential relationship between BBB disruption and impaired microglial function, whereby increasing BBB permeability led to a downregulation of microglial P2RY12 expression, thereby impairing microglial protection against cerebrovascular injury. Understanding these mechanisms may contribute to the developing of therapeutic strategies for diabetes-related neurological complications.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400482"},"PeriodicalIF":0.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optical Based Methods for Water Monitoring in Biological Tissue. 生物组织中水分监测的光学方法。
Journal of biophotonics Pub Date : 2025-01-24 DOI: 10.1002/jbio.202400438
Sergei Perkov, Julijana Cvjetinovic, Altynai Sydygalieva, Sergey Gorodkov, Guoqiang Li, Dmitry Gorin
{"title":"Optical Based Methods for Water Monitoring in Biological Tissue.","authors":"Sergei Perkov, Julijana Cvjetinovic, Altynai Sydygalieva, Sergey Gorodkov, Guoqiang Li, Dmitry Gorin","doi":"10.1002/jbio.202400438","DOIUrl":"https://doi.org/10.1002/jbio.202400438","url":null,"abstract":"<p><p>Skin homeostasis is strongly dependent on its hydration levels, making skin water content measurement vital across various fields, including medicine, cosmetology, and sports science. Noninvasive diagnostic techniques are particularly relevant for clinical applications due to their minimal risk of side effects. A range of optical methods have been developed for this purpose, each with unique physical principles, advantages, and limitations. This review provides an in-depth examination of optical techniques such as diffuse reflectance spectroscopy, optoacoustic spectroscopy, optoacoustic tomography, hyperspectral imaging, and Raman spectroscopy. We explore their efficacy in noninvasive monitoring of skin hydration and edema, which is characterized by an increase in interstitial fluid. By comparing the parameters, sensitivity, and clinical applications of these techniques, this review offers a comprehensive understanding of their potential to enhance diagnostic precision and improve patient care.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400438"},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of Microcirculation and Microrheological Parameters of Blood in Patients With Type 2 Diabetes Mellitus Using Biophotonics Techniques. 生物光子学技术评价2型糖尿病患者血液微循环和微流变参数。
Journal of biophotonics Pub Date : 2024-12-16 DOI: 10.1002/jbio.202400485
D A Umerenkov, P B Ermolinskiy, A E Lugovtsov, A A Fabrichnova, Yu I Gurfinkel, L I Dyachuk, A V Priezzhev
{"title":"Assessment of Microcirculation and Microrheological Parameters of Blood in Patients With Type 2 Diabetes Mellitus Using Biophotonics Techniques.","authors":"D A Umerenkov, P B Ermolinskiy, A E Lugovtsov, A A Fabrichnova, Yu I Gurfinkel, L I Dyachuk, A V Priezzhev","doi":"10.1002/jbio.202400485","DOIUrl":"https://doi.org/10.1002/jbio.202400485","url":null,"abstract":"<p><p>In this study, the parameters of blood microcirculation and microrheology were measured using the methods of laser aggregometry and optical tweezers in vitro, as well as the method of digital capillaroscopy in vivo. It was shown that in patients suffering from type 2 diabetes mellitus, an increase in the number of RBC aggregates passing through the narrow capillaries leads to a significant decrease in the velocity of the capillary blood flow, which can be explained by the increased viscosity of the whole blood and decreased deformability of RBCs. Also, for the group of patients, a statistically significant increase in the rate of RBC aggregation and the hydrodynamic strength of aggregates, RBC aggregation and disaggregation forces were observed compared to the control group. We have demonstrated the possibility of using these methods to assess changes in microrheological and microcirculatory parameters of the blood.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400485"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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