Effects of Multiple Near-Infrared LEDs (700, 850, and 980 nm) CW-PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts

IF 2 3区物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of Biophotonics Pub Date : 2025-04-03 DOI:10.1002/jbio.70015

Simone Sleep, Deanne H. Hryciw, Laurence J. Walsh, Eliza Ranjit, Nifty Tomy, Praveen R. Arany, Roy George

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Abstract

Background and Aim

This study evaluated mitochondrial and osteogenic activity in MG-63 pre-osteoblastic cells after photobiomodulation (PBM) using multiple near-infrared LED sources (Nuralyte) emitting wavelengths from 700 to 1100 nm.

Materials and Methods

MG-63 cells were irradiated daily for 3, 5, or 7 days with energy densities of 5.3 J/cm² (30 s, optimal dose) and 10.6 J/cm² (60 s, high dose). Mitochondrial function was assessed using the XF Seahorse analyzer, and gene expression of osteogenic markers was analyzed.

Results

Maximal mitochondrial oxygen consumption rate (OCR) significantly decreased at the optimal dose but increased at the high dose (p < 0.001) in 5-day irradiated cultures. Upregulation of osteogenic markers (OCN, OPN, BMP-2, COL-1, RUNX2) occurred after 3–5 consecutive days of irradiation, with greater activation at the optimal dose.

Conclusion

MG-63 cells respond to PBM using MNI-LEDs (700, 850, 980 nm) by modulating mitochondrial respiration and boosting bone-related gene expression in a dose- and time-dependent manner.

Abstract Image

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多种近红外 LED（700、850 和 980 纳米）CW-PBM 对 MG63 骨母细胞线粒体呼吸和基因表达的影响。

背景与目的：本研究利用多个波长为700 ~ 1100nm的近红外LED光源（Nuralyte）进行光生物调节（PBM）后，评估MG-63成骨前细胞的线粒体和成骨活性。材料和方法：MG-63细胞每天以5.3 J/cm2 （30 s，最佳剂量）和10.6 J/cm2 （60 s，高剂量）的能量密度照射3、5或7天。使用XF海马分析仪评估线粒体功能，并分析成骨标志物的基因表达。结果：最大线粒体耗氧率（OCR）在最佳剂量下显著降低，而在高剂量下显著升高(p)。结论：MG-63细胞通过MNI-LEDs（700、850、980 nm）通过调节线粒体呼吸和促进骨相关基因表达对PBM产生反应，并呈剂量和时间依赖性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biophotonics 生物-生化研究方法

CiteScore

5.70

自引率

7.10%

发文量

248

审稿时长

1 months

期刊介绍： The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.