Journal of Biophotonics最新文献_第5页

Multi-Parameter Deep Learning Combined With Fluorescence Lifetime Imaging Microscopy for Non-Invasive and Label-Free Endometrial Cancer Screening 多参数深度学习结合荧光寿命成像显微镜用于无创无标签子宫内膜癌筛查。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-01-18 DOI: 10.1002/jbio.70228

Wenjia Zhao, Yonghui Xie, Jiacheng Zhou, Duantao Hou, Yulan Wang, Yuzhe Liu, Yiyan Fei, Jiong Ma, Lan Mi

{"title":"Multi-Parameter Deep Learning Combined With Fluorescence Lifetime Imaging Microscopy for Non-Invasive and Label-Free Endometrial Cancer Screening","authors":"Wenjia Zhao, Yonghui Xie, Jiacheng Zhou, Duantao Hou, Yulan Wang, Yuzhe Liu, Yiyan Fei, Jiong Ma, Lan Mi","doi":"10.1002/jbio.70228","DOIUrl":"10.1002/jbio.70228","url":null,"abstract":"<div>\u0000 \u0000 <p>This study presents a noninvasive and label-free endometrial cancer screening approach combining fluorescence lifetime imaging microscopy (FLIM) with a multi-parameter deep learning algorithm. The autofluorescence signals of NAD(P)H in cervical exfoliated cells of participants (<i>n</i> = 71) were detected by FLIM. Two key metabolic parameters—mean fluorescence lifetime (<i>t</i><sub><i>m</i></sub>) and protein-bound fraction (<i>a</i><sub>2</sub>)—were extracted using FLIM data, which reflect malignancy-associated biochemical changes. Single-parameter models based on individual parameters (<i>t</i><sub><i>m</i></sub> or <i>a</i><sub>2</sub>) and a multi-parameter model integrating both parameters were constructed. The results showed that single-parameter models suffered from sensitivity-specificity imbalance while the multi-parameter model leveraging feature complementarity significantly improved predictive performance—achieving 100% sensitivity and 92% specificity in external testing, with an AUC of 0.92, representing an improvement of 0.17–0.31 over single-parameter models. These findings suggest that combining multi-parameter deep learning strategies with FLIM holds strong potential for risk prediction of endometrial cancer, offering a new approach for noninvasive clinical screening.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elesclomol Diminishes Redox Imbalance in Peripheral Tissues of Mblac1 Knockout Mice 埃来氯莫尔降低Mblac1基因敲除小鼠外周组织氧化还原失衡。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-01-18 DOI: 10.1002/jbio.70224

Mehrnoosh Neghabi, Parisa Nategh, Anna M. Stauffer, Maureen K. Hahn, Randy D. Blakely, Mahsa Ranji

{"title":"Elesclomol Diminishes Redox Imbalance in Peripheral Tissues of Mblac1 Knockout Mice","authors":"Mehrnoosh Neghabi, Parisa Nategh, Anna M. Stauffer, Maureen K. Hahn, Randy D. Blakely, Mahsa Ranji","doi":"10.1002/jbio.70224","DOIUrl":"10.1002/jbio.70224","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To determine whether loss of <i>Mblac1</i>, a gene implicated in copper metabolism and Alzheimer's disease, causes systemic mitochondrial redox imbalance and whether treatment with the copper chaperone elesclomol (ES) can restore this balance.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We employed 3D cryoimaging to quantify redox ratio (RR) in kidneys and livers of <i>Mblac1</i> knockout (KO) mice and their wildtype (WT) littermates. Mice of both sexes were administered either vehicle control (C) or ES (10 mg/kg, i.p.).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>KO tissues exhibited reduced RR, indicating an oxidized metabolic state. ES treatment recovered 77% of the RR deficit in kidneys and 48% in livers, restoring RR to WT levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p><i>Mblac1</i> deletion disrupts mitochondrial redox homeostasis in peripheral tissues, while ES partially reverses this imbalance by restoring Cu(I)-dependent metabolism.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Significance</h3>\u0000 \u0000 <p>Optical metabolic imaging reveals <i>Mblac1</i> as a key regulator of systemic redox balance and supports ES as a potential therapy for Cu-linked metabolic dysfunctions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confocal Raman Microscopy to Study Silicone Breast Implant's Early-Stage Degradation in Reconstructive Surgery 用共聚焦拉曼显微镜研究硅胶乳房假体在乳房再造手术中的早期退化。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-01-18 DOI: 10.1002/jbio.202500510

Duong Phu Le, Richard Younes, Frédéric Cuisinier, Frédéric Bodin, Nadia Bahlouli, Simon Chatelin, Hamideh Salehi

{"title":"Confocal Raman Microscopy to Study Silicone Breast Implant's Early-Stage Degradation in Reconstructive Surgery","authors":"Duong Phu Le, Richard Younes, Frédéric Cuisinier, Frédéric Bodin, Nadia Bahlouli, Simon Chatelin, Hamideh Salehi","doi":"10.1002/jbio.202500510","DOIUrl":"10.1002/jbio.202500510","url":null,"abstract":"<div>\u0000 \u0000 <p>The adverse effects of breast implants, especially silicone breast implants, are increasingly being shown. In addition to complications related to surgical technique and individual patient factors, the implant aging is suspected of being one of the main causes. This study employs the Raman technique, a label-free, non-destructive method that provides chemical composition information, to clarify the structure of the membrane and its gel-bleeding prevention function. The results identified two important markers—diphenylsiloxane and CH<sub>3</sub> vibration—that can distinguish the degradation state of silicone breast implants before and after implantation. In addition, two Raman bands at 1159 and 1524 cm<sup>−1</sup>, indicative of gel yellowing, were identified, providing potential insights into the permeation of external substances into the gel through the membrane. The results are consistent with previous observations and highlight the potential of deep learning models to determine degradation time thresholds based on these identified markers.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility of Depth-in-Color En Face Optical Coherence Tomography for Colorectal Polyp Classification Using Ensemble Learning and Score-Level Fusion 基于集成学习和分数级融合的深度彩色人脸光学相干断层扫描在结肠直肠息肉分类中的可行性。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-01-18 DOI: 10.1002/jbio.202500292

Andrew D. Thrapp, Sean D'Mello, Constantinos Pitris, Christos Photiou, Genevieve Lamphier, Erica Villareyna-Lopez, Anita Chung, Catriona Grant, Hinnerk Schulz-Hildenbrandt, Oscar Caravaca-Mora, Tiffany Miller, Du-Ri Song, Hamed Khalili, Norman S. Nishioka, Guillermo Tearney

{"title":"Feasibility of Depth-in-Color En Face Optical Coherence Tomography for Colorectal Polyp Classification Using Ensemble Learning and Score-Level Fusion","authors":"Andrew D. Thrapp, Sean D'Mello, Constantinos Pitris, Christos Photiou, Genevieve Lamphier, Erica Villareyna-Lopez, Anita Chung, Catriona Grant, Hinnerk Schulz-Hildenbrandt, Oscar Caravaca-Mora, Tiffany Miller, Du-Ri Song, Hamed Khalili, Norman S. Nishioka, Guillermo Tearney","doi":"10.1002/jbio.202500292","DOIUrl":"10.1002/jbio.202500292","url":null,"abstract":"<p>In colorectal cancer screening, accurate detection of precursor lesions is challenging due to their ambiguous surface appearance. Depth-sensitive optical coherence tomography (OCT) with deep learning may improve accuracy. OCT imaging was performed on polyps from 300 subjects. Depth was encoded (surface, mid, deep) in color to generate en face OCT projections. En face projections were then annotated. The projections were then used to train an ensemble network based on the malignant potential of polyps. The area under the curve (AUC) for detecting malignant potential of all polyps was 0.90, and for diminutive polyps (≤ 5 mm), it was 0.88. These results demonstrate a high degree of accuracy in classifying malignant potential ex vivo. Should these results hold in vivo, this algorithm would meet the ASGE's PIVI criteria for NPV, supporting clinical use of OCT for either a lower colon ‘diagnose and leave’ strategy and/or ‘resect and discard’ strategy for diminutive polyps.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12812504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomolecular Fingerprint of Crohn's Disease: A Comparative Raman Spectroscopic Study of Blood and Tissue Samples 克罗恩病的生物分子指纹：血液和组织样本的拉曼光谱比较研究。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-01-18 DOI: 10.1002/jbio.70219

M. Daniyal Ghauri, Maria Victoria Fernandez, Giovanni Santacroce, Sumedha Chanda, Irene Zammarchi, Ivan Capobianco, Sanathana Konugolu Venkata Sekar, Subrata Ghosh, Katarzyna Komolibus, Stefan Andersson-Engels, Marietta Iacucci, Rekha Gautam

{"title":"Biomolecular Fingerprint of Crohn's Disease: A Comparative Raman Spectroscopic Study of Blood and Tissue Samples","authors":"M. Daniyal Ghauri, Maria Victoria Fernandez, Giovanni Santacroce, Sumedha Chanda, Irene Zammarchi, Ivan Capobianco, Sanathana Konugolu Venkata Sekar, Subrata Ghosh, Katarzyna Komolibus, Stefan Andersson-Engels, Marietta Iacucci, Rekha Gautam","doi":"10.1002/jbio.70219","DOIUrl":"10.1002/jbio.70219","url":null,"abstract":"<p>Crohn's disease (CD) is typically diagnosed through endoscopy and histological analysis of biopsies. Currently, specific noninvasive biomarkers for accurate diagnosis and monitoring of CD remain unavailable. This study investigates Raman spectroscopy (RS) as a noninvasive diagnostic and monitoring tool for CD. Plasma and biopsy samples from 66 participants (55—CD, 11—controls) were analyzed to identify disease-specific molecular alterations. RS revealed significant spectral differences (<i>p</i> < 0.05) in amino acids, proteins, carbohydrates, and carotenoids. Decreased valine and arginine levels were consistent with mucosal damage and inflammation, supported by reduced carbohydrate signals indicating barrier impairment. Carotenoid depletion reflected oxidative stress-related inflammation. Correlations between plasma and tissue spectra suggested that plasma RS can reflect local tissue inflammation. Despite the small and heterogeneous cohort, RS detected biochemical shifts toward healthy profiles following biologic therapy. These findings support RS as a minimally invasive approach for detecting inflammation-associated molecular alterations, holding potential to guide personalized disease management.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12812440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photochemical Crosslinking of Eyelid Tissue by Trans-Conjunctival Exposure to UV-A Radiation: Implications for Biological Safety 经结膜暴露于UV-A辐射后眼睑组织的光化学交联：对生物安全的影响。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-01-18 DOI: 10.1002/jbio.202500458

Nadja E. Pop, Shuko Suzuki, Robert G. Tripon, Alexandra I. Manta, Kah Meng Lee, Marc Parisot, Andrei G. Cocisiu, Mark H. B. Radford, Damien G. Harkin, Traian V. Chirila

{"title":"Photochemical Crosslinking of Eyelid Tissue by Trans-Conjunctival Exposure to UV-A Radiation: Implications for Biological Safety","authors":"Nadja E. Pop, Shuko Suzuki, Robert G. Tripon, Alexandra I. Manta, Kah Meng Lee, Marc Parisot, Andrei G. Cocisiu, Mark H. B. Radford, Damien G. Harkin, Traian V. Chirila","doi":"10.1002/jbio.202500458","DOIUrl":"10.1002/jbio.202500458","url":null,"abstract":"<div>\u0000 \u0000 <p>A procedure based on exposure to ultraviolet-A (UV-A) radiation is in current development for treating pathological conditions of the eyelid generated through the laxity of the tissues. By irradiating the eyelid in the presence of a photosensitizer, photochemical crosslinking of tarsal collagen is induced, leading to its mechanical reinforcement, thus preventing further laxity increase. Here, we investigated potential radiation-induced damage to the target tissues. Ex vivo specimens of ovine tarsal plates were irradiated trans-conjunctivally with UV-A radiation (365 nm) in the presence of riboflavin at increasing irradiances. The specimens were processed histologically and analyzed. Except for episodic minor conjunctival epithelial damage, there was no evidence of injury to resident cells, connective tissue, meibomian glands, or muscles. Based on in vitro histological analysis, the study demonstrated negligible tissue damage induced by irradiation with UV-A, thereby suggesting the crosslinking of tarsal collagen as a possible therapeutic approach for treating eyelid laxity.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ex Vivo Assessment of Keratinocytic Carcinomas Using a Fast Mid-Infrared Probe 使用快速中红外探针对角化细胞癌的体外评估。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-01-15 DOI: 10.1002/jbio.70226

Maria G. Vazquez de Vasquez, Rebecca C. Bradley, Charles L. Hitchcock, Angela S. Casey, Austin Cusick, James V. Coe, Ronald Siegle

引用次数: 0

Development of a Multi-Wavelength Photobiomodulation System for Soothing Skincare 多波长舒缓护肤光生物调节系统的研制

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-01-14 DOI: 10.1002/jbio.70220

Jinyu Chen, Zihao Zheng, Shanduan Zhang, Jianbo Sun

引用次数: 0

Misassignment of Fourier-Transform Infrared Spectra for Monitoring the Crosslinking of Collagen: A Critical Analysis 用于监测胶原交联的傅里叶变换红外光谱的错配：一个关键分析

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-01-14 DOI: 10.1002/jbio.70216

Traian V. Chirila, Shuko Suzuki, Idriss Blakey

引用次数: 0

High-Sensitivity SPR Biosensor for Tumor Cell Exosome Detection Based on Ge-Doped Fiber Core PCF 基于掺锗光纤芯PCF的肿瘤细胞外泌体检测高灵敏度SPR生物传感器

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-01-14 DOI: 10.1002/jbio.70222

Licui Ji, Honggang Pan, Hongli Dai, Qingcheng You, Yukun Zhu, Xing Wang, Xu Gao

{"title":"High-Sensitivity SPR Biosensor for Tumor Cell Exosome Detection Based on Ge-Doped Fiber Core PCF","authors":"Licui Ji, Honggang Pan, Hongli Dai, Qingcheng You, Yukun Zhu, Xing Wang, Xu Gao","doi":"10.1002/jbio.70222","DOIUrl":"https://doi.org/10.1002/jbio.70222","url":null,"abstract":"<div>\u0000 \u0000 <p>Current exosome detection technologies are constrained by limited sensitivity and refractive index (RI) detection ranges. To address this, this study proposes a biosensor based on germanium-doped photonic crystal fiber (PCF) and surface plasmon resonance (SPR) technology. Featuring a D-shaped structure with three layers of periodic pores, a germanium-doped core, and a gold film coating, this sensor extends the effective RI detection range to 1.00–1.45 RIU and achieves a peak wavelength sensitivity of 30 000 nm/RIU at 1500 nm. Simulation and optimization results demonstrate that this sensor can distinguish normal exosomes (1.35–1.38 RIU), tumor-derived exosomes (1.39–1.42 RIU), and pro-metastatic exosomes (1.43–1.45 RIU). Sensitivity significantly increases in the high-RI range (> 1.42 RIU), with corresponding maximum detection accuracies of 5 × 10<sup>−5</sup>, 1.6 × 10<sup>−5</sup>, and 3.33 × 10<sup>−6</sup> RIU, respectively. This sensor provides a label-free, high-performance detection tool for early postoperative micrometastasis surveillance, thereby enhancing cancer management efficacy.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145964416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0