Endothelial c-Myc and Doxorubicin-Induced Metabolic Alterations: A Multi-Organ Optical Imaging Study

IF 2 3区物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of Biophotonics Pub Date : 2025-04-21 DOI:10.1002/jbio.70037

Parisa Nategh, Mehrnoosh Neghabi, Busenur Ceyhan, Jacqueline F. Machi, Homan A. Rahbar, Maya S. Rodriguez, Aline G. Santana, Claudia O. Rodrigues, Mahsa Ranji

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引用次数: 0

Abstract

Significance

Endothelial c-Myc plays a critical role in redox homeostasis, with its deletion potentially exacerbating oxidative stress, particularly, during chemotherapy.

Aim

To assess the metabolic impact of endothelial c-Myc knockout (KO) and Doxorubicin (DOXO) treatment on kidneys, hearts, and livers using 3D optical cryo-imaging.

Approach

Redox ratios (NADH/FAD) were analyzed in control (CT) and KO mice treated with DOXO or saline to evaluate mitochondrial function and oxidative states.

Results

KO tissues showed significant reductions in redox ratios, indicating an oxidized state, with kidneys exhibiting up to a 51.42% decrease. DOXO treatment further exacerbated oxidative stress in KO tissues, while CT groups demonstrated protective effects.

Conclusions

Endothelial c-Myc is crucial for redox balance and protection against chemotherapy-induced oxidative damage, offering insights for targeted therapeutic strategies.

查看原文本刊更多论文

内皮c-Myc和阿霉素诱导的代谢改变：一项多器官光学成像研究。

意义：内皮c-Myc在氧化还原稳态中起关键作用，其缺失可能会加剧氧化应激，特别是在化疗期间。目的：利用三维光学冷冻成像技术评估内皮细胞c-Myc敲除（KO）和多柔比星（DOXO）治疗对肾脏、心脏和肝脏代谢的影响。方法：分析对照组（CT）和经DOXO或生理盐水处理的KO小鼠的氧化还原比（NADH/FAD），以评估线粒体功能和氧化状态。结果：KO组织显示氧化还原比显著降低，表明氧化状态，肾脏显示高达51.42%的下降。DOXO处理进一步加重了KO组织的氧化应激，而CT组显示出保护作用。结论：内皮c-Myc对于氧化还原平衡和防止化疗诱导的氧化损伤至关重要，为靶向治疗策略提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biophotonics 生物-生化研究方法

CiteScore

5.70

自引率

7.10%

发文量

248

审稿时长

1 months

期刊介绍： The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.