Na Fang, Linjing Shi, Xiaoli Su, Rong Chen, Liwen Hu, Lianhuang Li, Xingfu Wang, Zanyi Wu, Jianxin Chen
{"title":"Texture Analysis of Fibrous Meningioma Using Label-Free Multiphoton Microscopy","authors":"Na Fang, Linjing Shi, Xiaoli Su, Rong Chen, Liwen Hu, Lianhuang Li, Xingfu Wang, Zanyi Wu, Jianxin Chen","doi":"10.1002/jbio.202400241","DOIUrl":"10.1002/jbio.202400241","url":null,"abstract":"<div>\u0000 \u0000 <p>Fibrous meningiomas, a common type of brain tumor, present surgical challenges due to their variable hardness, which is crucial for complete resection and patient prognosis. This study explores the use of label-free multiphoton microscopy (MPM) for the objective assessment of the texture of fibrous meningiomas. Fresh tumor samples from 20 patients were analyzed using both multichannel and lambda mode MPM, with quantitative image analysis algorithms determining collagen content and multi-peak spectral fitting providing additional optical collagen metrics. The study compared medium and hard fibrous meningiomas, utilizing receiver operating characteristic analysis to evaluate predictive performance. Microstructural features were clearly visualized, enabling accurate diagnosis. Collagen-related parameters significantly differentiated between moderate and hard tumors (<i>p</i> < 0.05). High predictive values were observed for collagen content, collagen-to-NADH-free ratio, and collagen-to-FAD ratio (AUC = 0.748–0.839). A multivariate logistic model combining these biomarkers significantly improved diagnostic accuracy (AUC = 0.907). The findings suggest that MPM, with its ability to visualize and quantify microstructures such as collagen and cells without the need for staining, holds strong potential for rapid, objective, and accurate assessment of tumor texture during neurosurgery. The integration of MPM with multiphoton endoscopy paves the way for potential in vivo applications in the future.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142741870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detecting Collagen by Machine Learning Improved Photoacoustic Spectral Analysis for Breast Cancer Diagnostics: Feasibility Studies With Murine Models","authors":"Jiayan Li, Lu Bai, Yingna Chen, Junmei Cao, Jingtao Zhu, Wenxiang Zhi, Qian Cheng","doi":"10.1002/jbio.202400371","DOIUrl":"10.1002/jbio.202400371","url":null,"abstract":"<p>Collagen, a key structural component of the extracellular matrix, undergoes significant remodeling during carcinogenesis. However, the important role of collagen levels in breast cancer diagnostics still lacks effective in vivo detection techniques to provide a deeper understanding. This study presents photoacoustic spectral analysis improved by machine learning as a promising non-invasive diagnostic method, focusing on exploring collagen as a salient biomarker. Murine model experiments revealed more profound associations of collagen with other cancer components than in normal tissues. Moreover, an optimal set of feature wavelengths was identified by a genetic algorithm for enhanced diagnostic performance, among which 75% were from collagen-dominated absorption wavebands. Using optimal spectra, the diagnostic algorithm achieved 72% accuracy, 66% sensitivity, and 78% specificity, surpassing full-range spectra by 6%, 4%, and 8%, respectively. The proposed photoacoustic methods examine the feasibility of offering valuable biochemical insights into existing techniques, showing great potential for early-stage cancer detection.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400371","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiaxin Zhang, Horst Wallrabe, Karsten Siller, Brian Mbogo, Thomas Cassidy, Shagufta Rehman Alam, Ammasi Periasamy
{"title":"Measuring Metabolic Changes in Cancer Cells Using Two-Photon Fluorescence Lifetime Imaging Microscopy and Machine-Learning Analysis","authors":"Jiaxin Zhang, Horst Wallrabe, Karsten Siller, Brian Mbogo, Thomas Cassidy, Shagufta Rehman Alam, Ammasi Periasamy","doi":"10.1002/jbio.202400426","DOIUrl":"10.1002/jbio.202400426","url":null,"abstract":"<p>Two-photon (2P) fluorescence lifetime imaging microscopy (FLIM) was used to track cellular metabolism with drug treatment of auto-fluorescent coenzymes NAD(P)H and FAD in living cancer cells. Simultaneous excitation at 800 nm of both coenzymes was compared with traditional sequential 740/890 nm plus another alternative of 740/800 nm, before and after adding doxorubicin in an imaging time course. Changes of redox states at single cell resolution were compared by three analysis methods: our recently introduced fluorescence lifetime redox ratio (FLIRR: NAD(P)H-<i>a</i>\u0000 <sub>2</sub>%/FAD-<i>a</i>\u0000 <sub>1</sub>%), machine-learning (ML) algorithms using principal component (PCA) and non-linear multi-Feature autoencoder (AE) analysis. While all three led to similar biological conclusions (early drug response), the ML models provided statistically the most robust significant results. The advantage of the single 800 nm excitation of both coenzymes for metabolic imaging in above mentioned analysis is demonstrated.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400426","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kevin Saruni Tipatet, Katie Hanna, Liam Davison-Gates, Mario Kerst, Andrew Downes
{"title":"Subtype-Specific Detection in Stage Ia Breast Cancer: Integrating Raman Spectroscopy, Machine Learning, and Liquid Biopsy for Personalised Diagnostics","authors":"Kevin Saruni Tipatet, Katie Hanna, Liam Davison-Gates, Mario Kerst, Andrew Downes","doi":"10.1002/jbio.202400427","DOIUrl":"10.1002/jbio.202400427","url":null,"abstract":"<p>This study explores the integration of Raman spectroscopy (RS) with machine learning for the early detection and subtyping of breast cancer using blood plasma samples. We performed detailed spectral analyses, identifying significant spectral patterns associated with cancer biomarkers. Our findings demonstrate the potential for classifying the four major subtypes of breast cancer at stage Ia with an average sensitivity and specificity of 90% and 95%, respectively, and a cross-validated macro-averaged area under the curve (AUC) of 0.98. This research highlights efforts to integrate vibrational spectroscopy with machine learning, enhancing cancer diagnostics through a non-invasive, personalised approach for early detection and monitoring disease progression. This study is the first of its kind to utilise RS and machine learning to classify the four major breast cancer subtypes at stage Ia.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400427","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enhancing Spatial Resolution of Three-Dimensional Pulse Waves Through Advanced Photometric Stereo Techniques","authors":"Jiuai Sun, Kai Li, Zengkai Li, Mingji Zhang, Zhonghang Wu, Dida Zhang","doi":"10.1002/jbio.202400448","DOIUrl":"10.1002/jbio.202400448","url":null,"abstract":"<div>\u0000 \u0000 <p>Three-dimensional pulse wave's morphologies are essential biomarkers for assessing cardiovascular functionality. However, existing methods only provide sparse amplitude representations, limiting their diagnostic potential. This study employs a photometric stereo approach to enhance the spatial resolution of pulse waves by capturing video footage of skin surface micro-vibrations induced by blood volume fluctuations in underlying arteries. This non-invasive imaging modality enables the reconstruction of three-dimensional pulse waves and enriches our understanding of their spatial and temporal characteristics. By visualizing and analyzing the captured data, we gained new insights into the physiological origins of the optical signals reflected from the skin surface and their dynamic features, which are critical for evaluating cardiovascular health. This study has potential to advance new biomarkers for cardiovascular function assessment and improve the accuracy of diagnostic tools.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Przemysław Mitura, Wiesław Paja, Bartosz Klebowski, Paweł Płaza, Krzyszof Bar, Grzegorz Młynarczyk, Joanna Depciuch
{"title":"Urine Analysed by FTIR, Chemometrics and Machine Learning Methods in Determination Spectroscopy Marker of Prostate Cancer in Urine","authors":"Przemysław Mitura, Wiesław Paja, Bartosz Klebowski, Paweł Płaza, Krzyszof Bar, Grzegorz Młynarczyk, Joanna Depciuch","doi":"10.1002/jbio.202400278","DOIUrl":"10.1002/jbio.202400278","url":null,"abstract":"<div>\u0000 \u0000 <p>Prostate-specific antigen (PSA) is the most commonly used marker of prostate cancer. However, nearly 25% of men with elevated PSA levels do not have cancer and nearly 20% of patients with prostate cancer have normal serum PSA levels. Therefore, in this study, Fourier transform infrared (FTIR) spectroscopy was investigated as a new tool for detection of prostate cancer from urine. Obtained results showed higher levels of glucose, urea and creatinine in urine collected from patients with prostate cancer than that in control. Principal component analysis (PCA) was not noticed possibility of differentiation urine collected from healthy and nonhealthy patients. However, machine learning algorithms showed 0.90 accuracy and precision of FTIR in detection of prostate cancer from urine. We showed that wavenumbers at 1614 cm<sup>−1</sup> and 2972 cm<sup>−1</sup> were candidates for prostate cancer spectroscopy markers. Importantly, these FTIR markers correlated with Gleason score, PSA and mpMRI PI-RADS category.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Deep Learning-Based Approach to Characterize Skull Physical Properties: A Phantom Study","authors":"Deepika Aggrawal, Loïc Saint-Martin, Rayyan Manwar, Amanda Siegel, Dan Schonfeld, Kamran Avanaki","doi":"10.1002/jbio.202400131","DOIUrl":"10.1002/jbio.202400131","url":null,"abstract":"<p>Transcranial ultrasound imaging is a popular method to study cerebral functionality and diagnose brain injuries. However, the detected ultrasound signal is greatly distorted due to the aberration caused by the skull bone. The aberration mechanism mainly depends on thickness and porosity, two important skull physical characteristics. Although skull bone thickness and porosity can be estimated from CT or MRI scans, there is significant value in developing methods for obtaining thickness and porosity information from ultrasound itself. Here, we extracted various features from ultrasound signals using physical skull-mimicking phantoms of a range of thicknesses with embedded porosity-mimicking acoustic mismatches and analyzed them using machine learning (ML) and deep learning (DL) models. The performance evaluation demonstrated that both ML- and DL-trained models could predict the physical characteristics of a variety of skull phantoms with reasonable accuracy. The proposed approach could be expanded upon and utilized for the development of effective skull aberration correction methods.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Letícia C. S. Santos, Landulfo Silveira Jr, Marcos T. T. Pacheco
{"title":"Raman Spectroscopic Analysis of Urinary Creatine and Phosphate in Athletes: Pre- and Post-Training Assessment","authors":"Letícia C. S. Santos, Landulfo Silveira Jr, Marcos T. T. Pacheco","doi":"10.1002/jbio.202400210","DOIUrl":"10.1002/jbio.202400210","url":null,"abstract":"<div>\u0000 \u0000 <p>The aim of this study was to detect biochemical components in the urine of bodybuilders who ingested creatine pretraining compared to individuals who did not ingest creatine after physical exercise using Raman spectroscopy. Twenty volunteers practicing bodybuilding were selected to collect pre- and post-training urine samples, where 10 volunteers ingested creatine 30 min before pretraining urine collection (creatine group), and 10 did not (control group). The samples were subjected to Raman spectroscopy, and the spectra of both creatine and control groups and the difference (post—pre) for both groups were analyzed. Principal component analysis (PCA) technique was applied to the samples. The results showed peaks of creatine and phosphate in urine after training (creatine post-training group), suggesting that part of the creatine was absorbed and metabolized, and part was excreted. Raman spectroscopy could be applied to detect biocompounds in urine, such as unmetabolized creatine and phosphate.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hao Wang, Sisi Guo, Ruoyu Zhang, Jing Yao, Wen Tian, Jianfeng Wang
{"title":"Feasibility Study of Label-Free Raman Spectroscopy for Parathyroid Gland Identification","authors":"Hao Wang, Sisi Guo, Ruoyu Zhang, Jing Yao, Wen Tian, Jianfeng Wang","doi":"10.1002/jbio.202400220","DOIUrl":"10.1002/jbio.202400220","url":null,"abstract":"<div>\u0000 \u0000 <p>We aim to evaluate the feasibility of Raman spectroscopy for parathyroid gland (PG) identification during thyroidectomy. Using a novel side-viewing handheld Raman probe, a total of 324 Raman spectra of four tissue types (i.e., thyroid, lymph node, PG, and lipid) commonly encountered during thyroidectomy were rapidly (< 3 s) acquired from 80 tissue sites (thyroid [<i>n</i> = 10], lymph node [<i>n</i> = 10], PG [<i>n</i> = 40], lipid [<i>n</i> = 20]) of 10 euthanized Wistar rats. Two partial least-squares (PLS)-discriminant analysis (DA) detection models were developed, differentiating the lipid and nonlipid (i.e., thyroid, lymph node, and PG) tissues with an accuracy of 100%, and PG, lymph node, and thyroid could be detected with an accuracy of 98.4%, 93.9%, and 95.4% respectively. This work demonstrates the feasibility of Raman spectroscopy technique for PG identification and protection during thyroidectomy at the molecular level.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sien Li, Fei Ma, Fen Yan, Xiwei Dong, Yanfei Guo, Jing Meng, Hongjuan Liu
{"title":"SFNet: Spatial and Frequency Domain Networks for Wide-Field OCT Angiography Retinal Vessel Segmentation","authors":"Sien Li, Fei Ma, Fen Yan, Xiwei Dong, Yanfei Guo, Jing Meng, Hongjuan Liu","doi":"10.1002/jbio.202400420","DOIUrl":"10.1002/jbio.202400420","url":null,"abstract":"<div>\u0000 \u0000 <p>Automatic segmentation of blood vessels in fundus images is important to assist ophthalmologists in diagnosis. However, automatic segmentation for Optical Coherence Tomography Angiography (OCTA) blood vessels has not been fully investigated due to various difficulties, such as vessel complexity. In addition, there are only a few publicly available OCTA image data sets for training and validating segmentation algorithms. To address these issues, we constructed a wild-field retinal OCTA segmentation data set, the Retinal Vessels Images in OCTA (REVIO) dataset. Second, we propose a new retinal vessel segmentation network based on spatial and frequency domain networks (SFNet). The proposed model are tested on three benchmark data sets including REVIO, ROSE and OCTA-500. The experimental results show superior performance on segmentation tasks compared to the representative methods.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}