Journal of Biophotonics最新文献_第4页

Intensity Variance-Guided Automated Robotic Optical Coherence Tomography System for Ophthalmic Applications 用于眼科应用的强度方差引导自动机器人光学相干断层扫描系统

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-08-19 DOI: 10.1002/jbio.70343

Hang Su, Jie Zhang, Maoyuan Qu, Xiru Gao, Hongqin Chen, Congyu Hu, Pengfei Song, Xingchen Ji, Yikai Su

{"title":"Intensity Variance-Guided Automated Robotic Optical Coherence Tomography System for Ophthalmic Applications","authors":"Hang Su, Jie Zhang, Maoyuan Qu, Xiru Gao, Hongqin Chen, Congyu Hu, Pengfei Song, Xingchen Ji, Yikai Su","doi":"10.1002/jbio.70343","DOIUrl":"https://doi.org/10.1002/jbio.70343","url":null,"abstract":"<div>\u0000 \u0000 <p>Optical coherence tomography (OCT) enables non-invasive volumetric retinal imaging. Conventional tabletop and handheld systems rely on skilled operators and patient cooperation. Mobile robot-assisted OCT (RAOCT) systems can address these challenges, but many depend on complex visual servo modules for low-latency eye tracking. We present an intensity variance-guided RAOCT system integrated on a wheeled mobile platform. The system uses one depth camera for coarse eye localization and a single pupil camera for real-time tracking. By calibrating a lookup table between image variance and distance within the near-eye region, the pupil camera provides indirect depth estimation for robotic servoing. A motorized reference arm, an electrically tunable lens, and an automated polarization controller further enable image-quality optimization. Experiments demonstrated 103.50 μm axial and 20.46 μm lateral tracking accuracy. The pupil-camera response time was 10.53 ms. Automated retinal OCT imaging was achieved, demonstrating the system's potential for point-of-care diagnostics in resource-limited environments.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multispectral Photoacoustic Imaging Detects Altered Hepatic Hemodynamics and Lipid in Early STZ-Induced Type 1 Diabetic Liver Injury 多光谱光声成像检测早期stz诱导的1型糖尿病肝损伤的肝脏血流动力学和脂质改变

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-08-18 DOI: 10.1002/jbio.70344

Tingting Qiu, Jiazhi Cao, Xijing Yang, Yan Luo, Hong Wang, Wenwu Ling

引用次数: 0

In Vivo Monitoring of Facial Acne Development by Using Multimodal Functional OCT 利用多模态功能OCT在体内监测面部痤疮的发展

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-08-13 DOI: 10.1002/jbio.70337

Zhengshuyi Feng, Jinpeng Liao, Tianyu Zhang, Guangyu Zhang, Alison M. Layton, Tarl Prow, Chunhui Li, Zhihong Huang

{"title":"In Vivo Monitoring of Facial Acne Development by Using Multimodal Functional OCT","authors":"Zhengshuyi Feng, Jinpeng Liao, Tianyu Zhang, Guangyu Zhang, Alison M. Layton, Tarl Prow, Chunhui Li, Zhihong Huang","doi":"10.1002/jbio.70337","DOIUrl":"https://doi.org/10.1002/jbio.70337","url":null,"abstract":"<div>\u0000 \u0000 <p>Acne is a common inflammatory skin condition and frequently leads to long-term adverse clinical outcomes including scarring and pigmentation, negatively impacting Health Related Quality of Life (HRQoL). Monitoring of acne progression can improve understanding of lesion evolution, support evaluation of treatment response and provide potential biomarkers for future investigation of acne sequelae. This study presents a multi-functional optical coherence tomography (OCT) protocol integrating structural imaging, OCT-based angiography (OCTA), and optical coherence elastography (OCE) for longitudinal in vivo monitoring of acne. Thirteen lesions were evaluated across eight time points over 18 days. Quantitative parameters, including Young's modulus and vascular metrics, captured biomechanical and microvascular changes during acne evolution. The system enables continuous multimodal assessment, showing that stiffness and vascular alterations reflect lesion progression. This pilot longitudinal study establishes a quantitative foundation for dermatological research and suggests that multimodal OCT monitoring may provide biomarkers for assessing lesion progression and long-term clinical outcomes.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linear Variable Filter-Based Wide-Field Hyperspectral Camera for Multiplex Immunoplasmonics Imaging 基于线性可变滤光片的多路免疫等离子成像宽视场高光谱相机。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-08-11 DOI: 10.1002/jbio.70341

Cécile Darviot, Michel Meunier

{"title":"Linear Variable Filter-Based Wide-Field Hyperspectral Camera for Multiplex Immunoplasmonics Imaging","authors":"Cécile Darviot, Michel Meunier","doi":"10.1002/jbio.70341","DOIUrl":"10.1002/jbio.70341","url":null,"abstract":"<p>Hyperspectral imaging combined with plasmonic nanoparticles offers powerful opportunities for nanoscale biological diagnostics, yet conventional push-broom systems are limited by slow acquisition and reduced spatial resolution. We report a wide-field hyperspectral imaging platform integrating a custom linear variable tunable filter (LVTF) with reflected light microscopy (RLM) for multiplexed plasmonic nanoparticle (NP) imaging. While LVTF-based systems have been used in industrial contexts, their adaptation to RLM microscopy enables high-resolution, high-contrast spectral imaging of NPs on biological samples. The system acquires data across 380–720 nm with 7–20 nm spectral resolution while preserving spatial detail. We demonstrate imaging of NPs bound to cancer cell membranes and classification of four distinct NP types. A trained support vector machine achieves an overall <i>F</i>-score of ~97%, significantly outperforming RGB-based approaches. Compared to push-broom systems, this platform provides faster acquisition, improved spatial resolution, and a compact, cost-effective solution for multiplex NP detection in bioimaging and immunoplasmonic applications.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70341","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148714929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Path Analysis of the Cancer Mueller Matrix: From Physical Decomposition to High-Dimensional Vector Mapping 癌症穆勒矩阵的路径分析：从物理分解到高维向量映射。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-08-11 DOI: 10.1002/jbio.70331

ChenChen Wang, Danfei Huang, ZhiYing Liu, RongWei Dai, Xiang Li, Yi Xie, Dong Song, Jinghui Hong

{"title":"Path Analysis of the Cancer Mueller Matrix: From Physical Decomposition to High-Dimensional Vector Mapping","authors":"ChenChen Wang, Danfei Huang, ZhiYing Liu, RongWei Dai, Xiang Li, Yi Xie, Dong Song, Jinghui Hong","doi":"10.1002/jbio.70331","DOIUrl":"10.1002/jbio.70331","url":null,"abstract":"<div><p>Subtle differences between cancerous and normal regions in unstained tissue sections limit the performance of conventional diagnostic methods. Mueller matrix polarimetry provides comprehensive information on tissue polarization responses; however, the intrinsic coupling of optical effects in the original matrix elements complicates direct histological interpretation. In this study, a pixel-level polarization dataset from clinical lung cancer and basal cell carcinoma sections is established to systematically compare low-dimensional physical decomposition and high-dimensional combination mapping for cancer-region identification. Conventional decomposition approaches exhibit limited discriminative capability and significant class overlap in complex tissues. To address this limitation, a vectorial metric norm spectrum incorporating multi-order features is developed, enabling enhanced representation of polarization characteristics. The proposed high-dimensional mapping framework achieves accurate differentiation between cancerous and normal regions and demonstrates strong performance in cross-validated evaluations. This work establishes a progressive strategy from physical decomposition to high-dimensional representation for optical-assisted pathological analysis.</p></div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148709251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic Therapy for Facial Rejuvenation Combined Amber LED and Infrared Laser Irradiation: A Pilot Study Comparing ALA and MAL 琥珀色LED和红外激光联合光动力治疗面部年轻化：ALA和MAL比较的初步研究。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-08-10 DOI: 10.1002/jbio.70335

Tassia Joi Martins, Juliana Teixeira Pedroso, Bruno Henrique Godoi, Juliana Guerra Pinto, Juliana Ferreira-Strixino

{"title":"Photodynamic Therapy for Facial Rejuvenation Combined Amber LED and Infrared Laser Irradiation: A Pilot Study Comparing ALA and MAL","authors":"Tassia Joi Martins, Juliana Teixeira Pedroso, Bruno Henrique Godoi, Juliana Guerra Pinto, Juliana Ferreira-Strixino","doi":"10.1002/jbio.70335","DOIUrl":"10.1002/jbio.70335","url":null,"abstract":"<p>The skin performs essential functions between the internal and external environments of the human body, such as protection against microorganisms, substances and radiation, maintenance of body temperature, prevention of excessive water loss, and production of vitamin D. However, many of these functions are reduced with aging and can be accentuated in photoaged skin. Photodynamic therapy (PDT) is a noninvasive technique used in the treatment of cancer, microbial infections, precancerous changes and for cosmetic purposes. Research on topical PDT suggests antibacterial, anti-inflammatory and immunomodulatory effects on keratinocytes, fibroblasts, sebaceous glands and hair follicles. Therefore, this pilot study evaluated PDT on the face using 5-aminolevulinic acid (ALA) and methyl 5-aminolevulinate (MAL), combined with amber LED and laser irradiation in women aged 40–55 years. PDT was effective in rejuvenating photoaged skin, reducing fine lines, smoothing wrinkles, and improving skin softness, firmness and sagging, with minimal side effects.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70335","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148709178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polarized Light Microscopy for Quantitative Assessment of Sarcomere-Scale Structural Order in Cardiac Tissue 偏振光显微镜定量评价心肌组织肌级结构秩序。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-08-10 DOI: 10.1002/jbio.70338

Gennadii Piavchenko, Artem Venediktov, Zoya Shamitko, Karina Urazova, Mariia Obelchakova, Evelina Khabarova, Anton Ershov, Natalia Kartashkina, Viktoria Grikh, Valerii Smirnov, Galina Ramenskaya, Alexander Yatskovskiy, Igor Meglinski

{"title":"Polarized Light Microscopy for Quantitative Assessment of Sarcomere-Scale Structural Order in Cardiac Tissue","authors":"Gennadii Piavchenko, Artem Venediktov, Zoya Shamitko, Karina Urazova, Mariia Obelchakova, Evelina Khabarova, Anton Ershov, Natalia Kartashkina, Viktoria Grikh, Valerii Smirnov, Galina Ramenskaya, Alexander Yatskovskiy, Igor Meglinski","doi":"10.1002/jbio.70338","DOIUrl":"10.1002/jbio.70338","url":null,"abstract":"<p>Polarization-resolved imaging provides a powerful, label-free means of visualizing anisotropic structural order in biological tissue, yet its quantitative exploitation remains limited in routine microscopy. Here, we present a polarization-resolved imaging approach based on polarized light microscopy (PLM) that enables automated, quantitative extraction of sarcomere-scale structural metrics from unstained cardiac tissue sections. Using experimental rat models of acute cardiorespiratory arrest as controlled test systems, we demonstrate that polarization-derived intensity profiles encode reproducible information on sarcomere length and band composition, with distinct patterns of structural compression observed across experimental conditions. Image-derived measurements were validated against conventional histological and immunohistochemical staining, highlighting the complementary value of polarization contrast for rapid, staining-free assessment of tissue anisotropy. Rather than addressing diagnostic specificity, this proof-of-concept study establishes PLM as a scalable quantitative imaging modality for polarization-resolved analysis of hierarchical structural order in biological tissue, with potential integration into multimodal imaging workflows in biomedical research.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70338","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148709254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Refractive Index Engineering for Human Corneal Tissue by Femtosecond Laser Two-Photon Cross-Linking 飞秒激光双光子交联用于人眼角膜组织折射率工程。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-08-06 DOI: 10.1002/jbio.70336

Pengfei Qi, Ziyan Zhou, Jinshi An, Haiyi Liu, Yifan Zhang, Hai Liu, Le Chang, Yanan Wu, Yan Wang, Olga Kosareva, Andrei Savel'ev, Lie Lin, Weiwei Liu

{"title":"Refractive Index Engineering for Human Corneal Tissue by Femtosecond Laser Two-Photon Cross-Linking","authors":"Pengfei Qi, Ziyan Zhou, Jinshi An, Haiyi Liu, Yifan Zhang, Hai Liu, Le Chang, Yanan Wu, Yan Wang, Olga Kosareva, Andrei Savel'ev, Lie Lin, Weiwei Liu","doi":"10.1002/jbio.70336","DOIUrl":"10.1002/jbio.70336","url":null,"abstract":"<div>\u0000 \u0000 <p>Clinical refractive surgeries for myopia correction generally entail stromal tissue removal with risks of postoperative complications. NIR femtosecond laser-induced corneal two-photon collagen cross-linking (two-photon CXL) through photochemical effects can lead to localized refractive index (RI) changes in the cornea, promising for non-ablative and non-incisional visual correction. However, previous studies mainly focused on hydrogels and animal corneal tissues, adopting a diffraction grating method that is difficult for biological tissue. Here we systematically investigate femtosecond laser-induced RI changes in human corneal tissue, demonstrate two-photon excitation of riboflavin leading to cross-linking, and employ direct interferometry to quantify RI changes. The maximum RI change is 3.80 ± 0.45 × 10<sup>−3</sup> in collagen hydrogel and 5.14 ± 0.75 × 10<sup>−3</sup> in corneal tissue. These findings illustrate that RI engineering by femtosecond laser two-photon CXL is promising for noninvasive refractive surgery.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148681589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile Optical Interrogation for Random Pulse Measurement: Characterization and Analysis of PPG Waveforms Using a Reflection-Based Sensor 随机脉冲测量的简易光学询问：使用基于反射的传感器表征和分析PPG波形。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-08-05 DOI: 10.1002/jbio.70340

Rajib Biswas, Subrata Sarkar

引用次数: 0

Blue Light Aminolevulinic Acid Photodynamic Therapy Did Not Induce Detectable CPD or 6–4PP Photolesions in Human Dermal Fibroblasts 蓝光氨基乙酰丙酸光动力疗法未诱导人皮肤成纤维细胞检测到CPD或6-4PP光损伤。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2026-08-05 DOI: 10.1002/jbio.70328

Julia Stolyar, Evan Austin, Olumide M. Arigbede, Jared Jagdeo

{"title":"Blue Light Aminolevulinic Acid Photodynamic Therapy Did Not Induce Detectable CPD or 6–4PP Photolesions in Human Dermal Fibroblasts","authors":"Julia Stolyar, Evan Austin, Olumide M. Arigbede, Jared Jagdeo","doi":"10.1002/jbio.70328","DOIUrl":"10.1002/jbio.70328","url":null,"abstract":"<p>Photodynamic therapy (PDT) is an established dermatologic therapeutic modality used to induce apoptosis within targeted tissue. Despite increasing clinical use, the genotoxic safety of blue light (BL) PDT has not yet been established. We examined whether combination BL (417 ± 5 nm) and 5-aminolevulinic acid (5-ALA) PDT induces cyclobutane pyrimidine dimers (CPDs) and 6–4 photoproducts (6–4PPs) in human dermal fibroblasts. CRL-2617 and AG-13145 fibroblasts were treated with 0, 0.5, or 1 mM 5-ALA, then irradiated with BL at 10, 30, or 45 J/cm<sup>2</sup>. Unlike robust photolesion formation in the UVB-irradiated positive controls, no BL PDT experimental condition produced CPD or 6–4PP signal exceeding the empirically validated assay lower limit of quantification (LLOQ; 1.5625 ng/mL). However, sub-LLOQ DNA damage cannot be excluded. The minimum detectable effect was 2.42 ng/mL, ~1.55-fold the LLOQ. Linear mixed-effects sensitivity analysis confirmed assay responsiveness, demonstrating clear separation between UVB and BL PDT signals.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13440324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148681573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0