Yujie Zheng, Limin Zhang, Weijie Song, Feng Gao, Dongyuan Liu
{"title":"Enhancing Functional Breast Imaging: A sCMOS Camera-Based Lock-in Implementation for Dynamic Tomography","authors":"Yujie Zheng, Limin Zhang, Weijie Song, Feng Gao, Dongyuan Liu","doi":"10.1002/jbio.202400473","DOIUrl":"10.1002/jbio.202400473","url":null,"abstract":"<p>Diffuse optical tomography (DOT) enables the in vivo quantification of tissue chromophores, specifically the discernment of oxy- and deoxy-hemoglobin (HbO and HbR, correspondingly). This specific criterion is useful in detecting and predicting early-stage neoadjuvant breast cancer treatment response. To address the issues of the limited channels in the fiber-dependent breast DOT system and limited signal-to-noise ratio in the camera-dependent systems, we hereby present a camera-based lock-in detection scheme to achieve dynamic DOT with improved SNR, which adopted orthogonal frequency division multiplexing technology. The evaluation of the system performance was conducted on tissue phantoms and neoplastic rats, and the results show that this system boasts the capability of executing parallel measurement utilizing a camera detector, enabling the achievement of highly sensitive, and dynamic tomography for breast screening applications.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Photobiomodulation Combined With Human Umbilical Cord Mesenchymal Stem Cells Modulates the Polarization of Microglia","authors":"Chunyan Ma, Hongyu Zhu, Yuanhao Cai, Na Li, Zhibo Han, Huancheng Wu, Hongli Chen","doi":"10.1002/jbio.202400468","DOIUrl":"10.1002/jbio.202400468","url":null,"abstract":"<div>\u0000 \u0000 <p>Neuroinflammation plays a key role in the development of neurodegenerative diseases, with microglia regulating this process through pro-inflammatory M1 and anti-inflammatory M2 phenotypes. Studies have shown that human umbilical cord mesenchymal stem cells (hUCMSCs) modulate neuroinflammation by secreting anti-inflammatory cytokines. Photobiomodulation (PBM), a non-invasive therapy, has demonstrated significant potential in alleviating neuroinflammation. This study examines the combined effects of PBM and hUCMSCs in an in vitro microglial inflammation model and an LPS-induced mouse model. The results show that PBM-pretreated hUCMSCs promoted M2 polarization and improved cognitive function in mice by downregulating the Notch signaling pathway, suggesting a promising new approach for treating neurodegenerative diseases.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weihang Geng, Qiming Xia, Mubin He, Yi Wang, Zheng Zheng, Haiyan Qin, Yalun Wang, Jian Feng, Jun Qian
{"title":"A Clinical Drug as the Three-Photon Fluorescence Probe for In Vivo Microscopic Imaging of Mouse Kidney","authors":"Weihang Geng, Qiming Xia, Mubin He, Yi Wang, Zheng Zheng, Haiyan Qin, Yalun Wang, Jian Feng, Jun Qian","doi":"10.1002/jbio.202400441","DOIUrl":"10.1002/jbio.202400441","url":null,"abstract":"<div>\u0000 \u0000 <p>Three-photon fluorescence (3PF) microscopy encounters significant challenges in biological research and clinical applications, primarily due to the limited availability of high-performance probes. We took a shortcut by exploring the excellent 3PF property of berberine hydrochloride (BH), a clinically utilized drug derived from the traditional Chinese medicine, Coptis. Capitalizing on its renal metabolism characteristics, we employed BH for in vivo 3PF microscopic imaging of the mouse kidney. This approach enabled high-resolution structural observation of renal tubules and facilitated the diagnosis of drug-induced acute kidney injury (AKI) based on renal tubule morphology. The analytical capabilities of 3PF microscopy for renal physiology and pathological diagnosis suggest its potential for future clinical applications.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José Ronildo Lins do Carmo Filho, Iásly Costa Lima, Paulo Goberlânio de Barros Silva, Ana Paula Negreiros Nunes Alves, Fabricio Bitu Sousa, Ana Maria Sampaio Assreuy, Mário Rogério Lima Mota
{"title":"Photobiomodulation Exerts Anti-Inflammatory and Antioxidant Effects Reducing the Development of Tumors Elicited by 4-NQO in the Mice Tongue","authors":"José Ronildo Lins do Carmo Filho, Iásly Costa Lima, Paulo Goberlânio de Barros Silva, Ana Paula Negreiros Nunes Alves, Fabricio Bitu Sousa, Ana Maria Sampaio Assreuy, Mário Rogério Lima Mota","doi":"10.1002/jbio.202400390","DOIUrl":"10.1002/jbio.202400390","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Evaluate the influence of photobiomodulation in a model of oral carcinogenesis induced by 4-nitroquinoline-n-oxide (4-NQO).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Subjective</h3>\u0000 \u0000 <p>Ninety-six Swiss mice received topical application of 1% 4-NQO on tongue dorsum, for 20 weeks. The tongue was subjected to photobiomodulation with red (71.4 J/cm<sup>2</sup>) and infrared laser (142.8 J/cm<sup>2</sup>) starting at week 0, 12, and 16. After 20 weeks, tongues were removed for the following analyzes: histological assessment, immunohistochemical reactions (cyclin D1/Ki-67/TGF-β1), quantification of MPO, n-AG, MDA, GSH, total proteins, TNF-α, IL-1β, and IL-6 levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>4-NQO showed significant increase in the frequency of carcinoma (<i>p</i> < 0.001), and in the immunostaining for cyclin D1/Ki-67/TGF-β1 (<i>p</i> < 0.005), along with increased levels of TNF- α, IL-1β, IL-6, MPO, n-AG, MDA, and total proteins (<i>p</i> < 0.001), that were reduced by photobiomodulation with red and infrared lasers (<i>p</i> < 0.005).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Photobiomodulation reduces tumor development, accompanied by reduced inflammatory cells and content of cytokines and oxidative markers associated with carcinogenesis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dynamic Neural Mechanisms Underlying Sustained Motor Training Post-Stroke: An fNIRS Investigation Employing Time-Varying ALFF and Functional Connectivity Analysis","authors":"Guanghu Zhang, Guangyue Zhu, Fan Hu, Yichen Jiang, Wenxi Li, Dongsheng Xu","doi":"10.1002/jbio.202400491","DOIUrl":"10.1002/jbio.202400491","url":null,"abstract":"<div>\u0000 \u0000 <p>Motor dysfunction of the upper limbs following a stroke predominantly arises from abnormal motor patterning caused by the disrupted balance of inter-cortical communication within motor-associated cortical regions. Temporal analysis offers a more precise reflection of the cortical functional state in affected patients. This study employed fNIRS to capture hemodynamic responses among 20 stroke patients and 19 healthy controls in both resting and Baduanjin task state. Including computing the coefficient of variation of fractional amplitude of low-frequency fluctuations (fALFF) for each channel, alongside extracting dynamic state metrics. Findings indicate that, during sustained motor tasks, stroke patients exhibit a diminished fALFF variability in targeted cortical regions; these individuals display a higher prevalence of low-intensity states and a lower prevalence of high-intensity states during task execution. These dynamic state attributes are significantly associated with scores on the upper limb motor function scale (FMA-UE), thereby proposing a time-domain perspective for investigating the underlying mechanisms of stroke-induced motor dysfunction.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ryan Muddiman, Sarah Harkin, Marion Butler, Bryan Hennelly
{"title":"Broadband CARS Hyperspectral Classification of Single Immune Cells","authors":"Ryan Muddiman, Sarah Harkin, Marion Butler, Bryan Hennelly","doi":"10.1002/jbio.202400382","DOIUrl":"10.1002/jbio.202400382","url":null,"abstract":"<p>Broadband CARS is a coherent Raman scattering technique that provides access to the full biological vibrational spectrum within milliseconds, facilitating the recording of widefield hyperspectral Raman images. In this work, BCARS hyperspectral images of unstained cells from two different cell lines of immune lineage (T cell [Jurkat] and pDCs [CAL-1]) were recorded and analyzed using multivariate statistical algorithms in order to determine the spectral differences between the cells. A classifier was trained which could distinguish the known cells with a 97% out-of-bag accuracy. The classifier was then applied to unlabeled samples containing a mixture of the two cell types on the same coverslip. This work demonstrates single-cell analysis of pDCs (CAL-1) and T cells (Jurkat) using BCARS. This approach enables an initial validation of cellular classification. We further demonstrate the capability of BCARS cell classification using single spectra of 5 ms acquisition time.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400382","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jonah A. Majumder, Danyang Cheng, Haiqiu Yang, Kenneth Laurita, Andrew M. Rollins, Deepak Saluja, Christine P. Hendon
{"title":"Near-Infrared Spectroscopic Mapping of the Human Epicardium","authors":"Jonah A. Majumder, Danyang Cheng, Haiqiu Yang, Kenneth Laurita, Andrew M. Rollins, Deepak Saluja, Christine P. Hendon","doi":"10.1002/jbio.202400464","DOIUrl":"10.1002/jbio.202400464","url":null,"abstract":"<div>\u0000 \u0000 <p>Epicardial catheter ablation is necessary to address ventricular tachycardia targets located far from the endocardium, but epicardial adipose tissue and coronary blood vessels can complicate ablation. We demonstrate that catheter-based near-infrared spectroscopy (NIRS) can identify these obstacles to guide ablation. Eighteen human ventricles were mapped ex vivo using NIRS catheters with optical source-detector separations (SDSs) of 0.6 and 0.9 mm. A logistic regression model trained from manually labeled spectra achieved mean area under the receiver operating characteristic curve (AUROC) of 0.907 (0.6 mm SDS) and 0.911 (0.9 mm SDS) in binary adipose detection. Novel optical indices for adipose detection were also proposed, achieving AUROCs of 0.881 (0.6 mm SDS) and 0.873 (0.9 mm SDS), while a blood-specific optical index achieved AUROC of 0.859 for vessel detection (0.9 mm SDS). These results suggest that catheter-based NIRS can detect adipose tissue and coronary vessels to improve efficacy and safety of epicardial ablation.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Measurement and Analysis of Optical Transmission Characteristics of the Human Skull","authors":"Peiquan Chen, Liang Zhou, Zhaohui Liu, Shuang Liu","doi":"10.1002/jbio.202400414","DOIUrl":"10.1002/jbio.202400414","url":null,"abstract":"<div>\u0000 \u0000 <p>The brain, as a vital part of central nervous system, receives approximately 25% of body's blood supply, making accurate monitoring of cerebral blood flow essential. While fNIRS is widely used for measuring brain physiology, complex tissue structure affects light intensity, spot size, and detection accuracy. Many studies rely on simulations with limited experimental validation. In this study, we used real adult skulls and agar to create a mimic model, building a transmission optical system with 13 wavelength filters and varying agar thicknesses. Peak intensity of transmitted light and size of scattered spot were measured at different wavelengths, and transmittance, total attenuation coefficient, and spot diameter enlargement of cranial mimics at different wavelengths were obtained. Results showed wavelengths below 550 nm struggled to penetrate the skull, while those above 700 nm penetrated deeper and diffused more. This suggests that short wavelengths capture epidermal PPG signals, whereas longer wavelengths detect both epidermal and intracranial signals.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khan Mohammad Khan, Hemant Krishna, Khageswar Sahu, Shovan K. Majumder
{"title":"An Artefact-Minimized Raman Probe for Analyzing Biological Tissues","authors":"Khan Mohammad Khan, Hemant Krishna, Khageswar Sahu, Shovan K. Majumder","doi":"10.1002/jbio.202400374","DOIUrl":"10.1002/jbio.202400374","url":null,"abstract":"<div>\u0000 \u0000 <p>Availability of a suitable tool for carrying out non-invasive measurement of Raman signatures <i>in situ</i>, from biological tissues having low Raman cross section is a clinically unmet need faced with manifold challenges. A Raman probe can prove to be an invaluable component of clinical Raman diagnostic systems. We present development of a Raman probe capable of measuring artefact free Raman spectra of biological tissues <i>in situ</i>. The developed probe uses a single lens for simultaneous illumination and collection of the Raman signal backscattered from the sample surface. This configuration ensures not only maximum overlapping of the illumination and collection volumes, ultimately leading to optimal throughput but also reduces the fiber-induced artefacts. The results show a superior performance of the developed Raman probe in measuring the Raman signatures from biological samples having lower Raman cross-sections, compared to that of the two commercially available Raman probes.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linda B. Neubrand, Xavier Attendu, Ton G. van Leeuwen
{"title":"Achieving High-Precision Attenuation Coefficient Measurement in Optical Coherence Tomography","authors":"Linda B. Neubrand, Xavier Attendu, Ton G. van Leeuwen","doi":"10.1002/jbio.202400395","DOIUrl":"10.1002/jbio.202400395","url":null,"abstract":"<p>In this study, we aim to validate the analytical Cramer-Rao lower bound (CRLB) equation for determining attenuation coefficients using a 1310 nm Optical Coherence Tomography (OCT) system. Our experimental results successfully confirm the validity of the equation, achieving unprecedented precision with a standard deviation below 0.01 mm<sup>−1</sup> for intralipid samples. Furthermore, we introduce a systematic framework for attaining high precision in OCT attenuation measurements.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400395","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}