{"title":"Prostate Cancer Characterization Based on Rapid SHG Imaging of Collagen Fiber Combined With Denoising Algorithm","authors":"Jia He, Xinpeng Huang, Yating Fang, Hong Chen, Chaoran Ban, Hua Zheng, Yuanying Zhang, Xiaoqin Zhu","doi":"10.1002/jbio.70233","DOIUrl":"10.1002/jbio.70233","url":null,"abstract":"<div>\u0000 \u0000 <p>Second harmonic generation (SHG) imaging technique can specifically image components such as collagen fibers, which provides diagnostic information for prostate cancer (PCa). Gleason grading serves as the primary criterion for assessing PCa. However, obtaining high signal-to-noise ratio (SNR) SHG images requires prolonged acquisition, limiting clinical feasibility. In this study, rapid SHG imaging was combined with a deep-learning-based denoising network, Selective Residual M-Net (SRMNet), to reconstruct high-fidelity images from low-SNR inputs. The denoising procedure markedly enhanced image quality while preserving collagen alignment features essential for quantitative assessment. The enhanced image fidelity enabled more robust extraction of collagen orientation metrics under low-SNR conditions, facilitating accurate assessment of stromal organization with reduced acquisition time. These results highlight that deep-learning-assisted SHG imaging provides an effective strategy for reliable collagen orientation analysis in PCa, supporting the development of rapid and objective optical histopathology.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146101083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ismail Erbas, Vikas Pandey, Navid Ibtehaj Nizam, Nanxue Yuan, Amit Verma, Tynan Young, John Williams, Margarida Barroso, Xavier Intes
{"title":"Enhancing Fluorescence Lifetime Imaging With Differential Transformer","authors":"Ismail Erbas, Vikas Pandey, Navid Ibtehaj Nizam, Nanxue Yuan, Amit Verma, Tynan Young, John Williams, Margarida Barroso, Xavier Intes","doi":"10.1002/jbio.70231","DOIUrl":"10.1002/jbio.70231","url":null,"abstract":"<div>\u0000 \u0000 <p>Conventional analytical methods for fluorescence lifetime imaging (FLI) require the instrument response function (IRF) alongside temporal fluorescence decay to accurately estimate lifetime parameters. While the IRF remains spatially and temporally invariant in microscopy, it exhibits significant pixel-wise spatial variation and temporal offsets at mesoscopic and macroscopic scales due to sample size and surface topography. Existing deep learning approaches, trained on limited synthetic or simplified experimental datasets, often fail to generalize to complex biological or in vivo imaging. Here, we introduce MFliNet, a deep learning framework working in accordance with the physics of photon time-of-flight deconvolution for accurate multi-exponential decay parameter estimation. Utilizing a Differential Transformer encoder–decoder architecture, MFliNet jointly processes temporal fluorescence decay and corresponding IRF inputs to correct topographical distortions in photon arrival distributions. Validation with tissue-mimicking phantoms and preclinical tumor models demonstrates exceptional robustness and precision, enabling reliable, real-time macroscopic FLI for complex biological and intraoperative applications.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146101129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jigar Lad, Erica Dao, Gabriella Gohla, Peter Lovrics, Qiyin Fang, Thomas J. Farrell, Michael J. Farquharson
{"title":"Time-Resolved Fluorescence and Diffuse Reflectance (TRF-DR) Spectroscopy for Heterogeneous Breast Tissue Classification and Tumor Margin Assessment","authors":"Jigar Lad, Erica Dao, Gabriella Gohla, Peter Lovrics, Qiyin Fang, Thomas J. Farrell, Michael J. Farquharson","doi":"10.1002/jbio.70230","DOIUrl":"10.1002/jbio.70230","url":null,"abstract":"<p>Re-excision rates remain high for early-stage breast cancer patients due to challenges in margin delineation during surgery, such as poorly defined tumor boundaries. Our group has developed a time-resolved fluorescence and diffuse reflectance (TRF-DR) spectroscopy system to assess tumor boundaries where tissue composition is heterogeneous. This study assesses system feasibility for classifying tumor in heterogeneous regions using weighted logistic regression and principal component analysis (PCA). A total of 4818 measurements from 73 frozen patient ex vivo samples were evaluated. Tissue measurement areas (1 mm × 1 mm) were pathologist-assigned percentages of low (< 25%), medium (25%–< 75%), and high (≥ 75%) for tumor, fibroglandular, or adipose composition. The model trained with four principal components (PCs) achieved sensitivity of 77% and specificity of 68%, comparable to current clinical techniques while being fast, portable, and economical. The TRF-DR system with weighted logistic regression demonstrates potential as an intraoperative margin assessment tool.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12862050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146101149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. R. Il'enkova, D. D. Rybnikov, A. I. Alekseeva, A. S. Kucheryavenko, S. V. Garnov, S. O. Yurchenko, V. V. Tuchin, K. I. Zaytsev, N. V. Chernomyrdin
{"title":"Heterogeneity and Birefringence of Soft Tissues Probed by the Polarization-Sensitive Terahertz Solid Immersion Microscopy","authors":"D. R. Il'enkova, D. D. Rybnikov, A. I. Alekseeva, A. S. Kucheryavenko, S. V. Garnov, S. O. Yurchenko, V. V. Tuchin, K. I. Zaytsev, N. V. Chernomyrdin","doi":"10.1002/jbio.70232","DOIUrl":"10.1002/jbio.70232","url":null,"abstract":"<div>\u0000 \u0000 <p>The polarization-sensitive THz solid immersion (SI) microscope is applied to quantify the heterogeneity and birefringence of freshly-excised rat tissues: the abdominal and tongue muscles, tendons of the flexor digitorum and extensor digitorum of the forelimb, Achilles tendon, abdominal aorta, and <i>Corpus callosum</i> of the brain. THz refractive indices of tissues are retrieved for the two orthogonal linear polarizations of the incident beam, which are collinear and transverse toward the fibrous structures. The observed THz birefringence, as high as a few percent, is attributed to tissue morphology relying on the hematoxylin and eosin (H&E)-stained histology. Expectedly, the THz refractive index is higher for the incident field collinear to the fibrous structures. The muscle tissues and <i>Corpus callosum</i> manifest the most pronounced birefringence, while that of tendon is sufficiently lower. Our findings uncover notable heterogeneity and birefringence of tissues; those should be accounted for when developing novel methods of THz biophotonics.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146047575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jae Yoon Park, Cha Kyung Youn, Sang-Mi Kang, Gisoon Choi, Seo Won Jang, Hea Ik Chung, Jae Min Kim, Ju Yeong Myeong, Ji Hye Yang, Cong Duc Nguyen, Yanghee You, Chang-Su Na, Kijoon Lee
{"title":"Local Microcirculation Level Changes During Laser Acupuncture Treatment of Rats With Osteoarthritis","authors":"Jae Yoon Park, Cha Kyung Youn, Sang-Mi Kang, Gisoon Choi, Seo Won Jang, Hea Ik Chung, Jae Min Kim, Ju Yeong Myeong, Ji Hye Yang, Cong Duc Nguyen, Yanghee You, Chang-Su Na, Kijoon Lee","doi":"10.1002/jbio.70234","DOIUrl":"10.1002/jbio.70234","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluates whether a deep-tissue microcirculation measurement can serve as an indicator of laser acupuncture treatment efficacy in an osteoarthritis (OA) animal model. Four groups of rats (normal, OA, OA10, and OA20, <i>n</i> = 6 each) were prepared where OA10 and OA20 mean treated groups with different light doses. Blood flow index (BFI) was measured at four time points (T0–T3) near acupoints GB34 and GB39 using a custom-made diffuse speckle contrast analysis (DSCA) system. BFI measurement showed an increase after OA induction and it remained elevated, whereas treatment groups exhibited a decrease in BFI. Spectral analysis revealed meaningful changes in the myogenic wave band, and a support vector machine classification demonstrated that incorporating BFI at T1 along with anatomical features significantly improved group separation accuracy, indicating a strong correlation between acute inflammatory response and anatomical indices.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146042430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amir Khansari, Seyed Mohsen Ranjbaran, Mohsin Zafar, Nafiseh Ghaffar Nia, Hossein Khodavirdi, Maria Tsoukas, Kamran Avanaki
{"title":"Multispectral Laser-Scanning Photoacoustic Microscopy With SRS-Generated Wavelengths for Skin Chromophore Characterization","authors":"Amir Khansari, Seyed Mohsen Ranjbaran, Mohsin Zafar, Nafiseh Ghaffar Nia, Hossein Khodavirdi, Maria Tsoukas, Kamran Avanaki","doi":"10.1002/jbio.70225","DOIUrl":"10.1002/jbio.70225","url":null,"abstract":"<p>We present a single pump-source, multispectral laser-scanning photoacoustic microscopy (MS-LS-PAM) platform that integrates stimulated Raman scattering (SRS)-based wavelength generation for high-resolution, label-free skin imaging. The system uses a compact, fiber-based light source to produce tunable excitation wavelengths from 532 to 571 nm, enabling precise spectral discrimination of key endogenous chromophores, oxyhemoglobin (HbO<sub>2</sub>), deoxyhemoglobin (Hb), melanin, and collagen, without external contrast agents. Spectral unmixing is performed using non-negative least squares (NNLS), facilitating quantitative analysis of chromophore distributions. In vivo experiments on B6 (pigmented) and SKH1 hairless (melanin deficient) mice demonstrated accurate chromophore separation, spatially resolved oxygen saturation (sO<sub>2</sub>) mapping, and clear visualization of collagen and melanin architectures.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12830072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146042416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"RETRACTION: Exploring the Use of Thermography for Monitoring Physiological Responses to Training in Division-I Collegiate Female Soccer Players","authors":"","doi":"10.1002/jbio.70229","DOIUrl":"10.1002/jbio.70229","url":null,"abstract":"<p>\u0000 <b>RETRACTION</b>: <span>C. J. Metoyer</span>, <span>J. R. Lever</span>, <span>V. Smith-Hale</span>, <span>C. Klaus</span>, <span>C. Ocobock</span>, <span>A. Huebner</span>, <span>M. McGinty</span>, <span>J. D. Hauenstein</span>, and <span>J. P. Wagle</span>, “ <span>Exploring the Use of Thermography for Monitoring Physiological Responses to Training in Division-I Collegiate Female Soccer Players</span>,” <i>Journal of Biophotonics</i> <span>18</span>, no. <span>8</span> (<span>2025</span>): e70016. https://doi.org/10.1002/jbio.70016.\u0000 </p><p>The above article, published online on 25 March 2025 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors; the <i>Journal of Biophotonics</i> editorial team under the leadership of the Editor-in-Chief, Prof. Jurgen Popp; and Wiley-VCH GmbH. Following publication, concerns were raised regarding errors in the data analysis and the numbering of citations. An investigation by the journal confirmed that errors in the statistical analysis and physiological findings fundamentally compromise the conclusions of the article. The retraction has been agreed to because the editors no longer have confidence in the results and conclusions presented.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70229","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146031960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rosane de Fátima Zanirato Lizarelli, Vitória Rocha Felca, Fabrizio dos Santos Cardoso, Ana Carolina Ribeiro de Oliveira, Simone Cecílio Hallak Regalo, Vanderlei Salvador Bagnato
{"title":"Protocol-Dependent Effects of Transcutaneous Blood Systemic Photobiomodulation on Inflammatory, Metabolic, and Hemostatic Markers","authors":"Rosane de Fátima Zanirato Lizarelli, Vitória Rocha Felca, Fabrizio dos Santos Cardoso, Ana Carolina Ribeiro de Oliveira, Simone Cecílio Hallak Regalo, Vanderlei Salvador Bagnato","doi":"10.1002/jbio.202500525","DOIUrl":"10.1002/jbio.202500525","url":null,"abstract":"<div>\u0000 \u0000 <p>Blood systemic photobiomodulation (BSP) is an integrative approach using low-intensity lasers or LEDs. This is a therapeutic strategy to modulate inflammatory, metabolic, and hemostatic biomarkers, promoting potentially beneficial systemic effects. Fifty-six female volunteers participated in this study that investigated the effects of different transcutaneous photobiomodulation protocols applied over the radial artery, using red and infrared light under different dosimetry. Volunteers were allocated into experimental groups, and biomarkers were evaluated. Results show that the IR-V group exhibited a significant increase in HDL levels after treatment. Regarding insulin, a significant reduction was observed in the IR-LD group after treatment; ferritin levels were significantly reduced in the LED group. No significant differences were found for the other biomarkers analyzed. These findings reinforce the importance of personalizing photobiomodulation protocols and suggest that specific wavelength- and dose-dependent parameters may lead to distinct systemic biochemical responses.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Johannes Pittrich, Georg von Köller, Christoph Dillitzer, Daniel Sandner, Ellen Emken, Julia Sistermanns, Zsuzsanna Wolf, Martin Schlegel, Gregor Weirich, Reinhard Kienberger, Oliver Hayden, Hristo Iglev
{"title":"Fabry-Pérot Microscopy for Improved Contrast Enhancement and Quantitative Phase Imaging","authors":"Johannes Pittrich, Georg von Köller, Christoph Dillitzer, Daniel Sandner, Ellen Emken, Julia Sistermanns, Zsuzsanna Wolf, Martin Schlegel, Gregor Weirich, Reinhard Kienberger, Oliver Hayden, Hristo Iglev","doi":"10.1002/jbio.70217","DOIUrl":"10.1002/jbio.70217","url":null,"abstract":"<p>Fabry-Pérot microscopy (FPM) is a label-free imaging technique that combines a microfluidic optical cavity with a tunable light source to enhance contrast and enable quantitative phase imaging. It integrates a fixed-length Fabry-Pérot cavity into a coated microfluidic channel and utilizes resonance-based interference to selectively highlight structures with specific optical thickness. This design preserves lateral resolution while providing > 20-fold contrast enhancement compared to conventional wide-field microscopy. Demonstrations with human oral epithelial cells, blood components, and <i>E. coli</i> show clear visualization of subcellular features and differentiation of cell types. Notably, FPM achieves imaging through spectral tuning rather than mechanical scanning, ensuring faster and more stable operation. The system is compatible with standard microscope optics and works under both static and flow conditions, offering significant potential for high-throughput cytometry, biological research, and in vitro diagnostics. These results establish FPM as a versatile extension of wide-field microscopy, enabling contrast-tunable, quantitative imaging of biomedical samples.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12812438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Use of High-Power Diode Laser in Neonatal Lingual Frenulum Release Surgery: Clinical Approach and Assessment of Healing in a Case Series","authors":"Adriana Cátia Mazzoni, Amanda Rafaelly Honório Mandetta, Manoela Domingues Martins, Cinthya Cosme Gutierrez Duran, Raquel Agnelli Mesquita-Ferrari, Anna Carolina Ratto Tempestini Horliana, Kristianne Porta Santos Fernandes, Lara Jansiski Motta, Sandra Kalil Bussadori","doi":"10.1002/jbio.70218","DOIUrl":"10.1002/jbio.70218","url":null,"abstract":"<div>\u0000 \u0000 <p>Ankyloglossia is a congenital condition characterized by restricted tongue mobility due to an abnormal lingual frenum, which may impair breastfeeding in newborns and infants. Early diagnosis and treatment are essential to prevent functional complications. This case series describes 35 newborns and infants with ankyloglossia who underwent frenectomy using a super-pulsed dual-wavelength diode laser. The procedures were performed using a diode laser operating at wavelengths of 810 nm (1.2 W) and 980 nm (1.4 W), delivering a final output power of 1.1 W, in super-pulsed mode (20 Hz frequency, 0.05 ms pulse width), with a 400-μm fiber optic tip. Healing and tongue mobility were evaluated immediately after surgery and at seven and 15 days. Most cases showed balanced tissue repair, absence of fibrosis, and improved tongue mobility, resulting in effective breastfeeding. Unfavorable outcomes were associated with external factors such as pathological healing history and poor adherence to postoperative care.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}