Journal of Biophotonics最新文献_第4页

Fast Multiphoton Microscopic Imaging Joint Image Super-Resolution for Automated Gleason Grading of Prostate Cancers 用于前列腺癌格里森自动分级的快速多光子显微成像联合图像超级分辨率

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-09-11 DOI: 10.1002/jbio.202400233

Xinpeng Huang, Qianqiong Wang, Jia He, Chaoran Ban, Hua Zheng, Hong Chen, Xiaoqin Zhu

{"title":"Fast Multiphoton Microscopic Imaging Joint Image Super-Resolution for Automated Gleason Grading of Prostate Cancers","authors":"Xinpeng Huang, Qianqiong Wang, Jia He, Chaoran Ban, Hua Zheng, Hong Chen, Xiaoqin Zhu","doi":"10.1002/jbio.202400233","DOIUrl":"10.1002/jbio.202400233","url":null,"abstract":"<div>\u0000 \u0000 <p>Gleason grading system is dependable for quantifying prostate cancer. This paper introduces a fast multiphoton microscopic imaging method via deep learning for automatic Gleason grading. Due to the contradiction between multiphoton microscopy (MPM) imaging speed and quality, a deep learning architecture (SwinIR) is used for image super-resolution to address this issue. The quality of low-resolution image is improved, which increased the acquisition speed from 7.55 s per frame to 0.24 s per frame. A classification network (Swin Transformer) was introduced for automated Gleason grading. The classification accuracy and Macro-F1 achieved by training on high-resolution images are respectively 90.9% and 90.9%. For training on super-resolution images, the classification accuracy and Macro-F1 are respectively 89.9% and 89.9%. It shows that super-resolution image can provide a comparable performance to high-resolution image. Our results suggested that MPM joint image super-resolution and automatic classification methods hold the potential to be a real-time clinical diagnostic tool for prostate cancer diagnosis.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental Study on the Process and Performance of Laser-Assisted Vascular Anastomosis Based on Response Surface Methodology 基于响应面方法的激光辅助血管吻合术过程和性能实验研究

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-09-05 DOI: 10.1002/jbio.202400217

Jun Huang, Mintao Yan, Yanyu Li, Tongyu Xu, Kehong Wang

引用次数: 0

Suction-Based Optical Coherence Elastography for the Biomechanical Characterization of Pathological Skin Conditions: A Pilot Study 基于吸力的光学相干弹性成像技术用于病理皮肤的生物力学特征描述：试点研究

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-09-04 DOI: 10.1002/jbio.202300314

L. van Haasterecht, L. Bartolini, J. M. I. Louter, P. J. González, F. B. Niessen, D. Iannuzzi, M. L. Groot, P. P. M. van Zuijlen

{"title":"Suction-Based Optical Coherence Elastography for the Biomechanical Characterization of Pathological Skin Conditions: A Pilot Study","authors":"L. van Haasterecht, L. Bartolini, J. M. I. Louter, P. J. González, F. B. Niessen, D. Iannuzzi, M. L. Groot, P. P. M. van Zuijlen","doi":"10.1002/jbio.202300314","DOIUrl":"10.1002/jbio.202300314","url":null,"abstract":"<p>Accurate characterization of mechanical properties is crucial in the evaluation of therapeutic effects for problematic skin conditions. A pilot study was carried out using a novel optical coherence elastography (OCE) device, combining mechanical characterization through suction-based deformation and imaging through optical coherence tomography. Using AI-assisted image segmentation and a power-law model, we were able to describe the mechanical behavior, comparing with measurements from the most commonly used commercial instrument (Cutometer) and subjective analyses of stiffness using the Patient and Observer Scar Assessment Scale. Twenty subjects were included with either keloids or hypertrophic scars. Measurements were fast and produced no discomfort. Mechanical and structural (epidermal thickness and rugosity) descriptors in pathologic skin conditions differed significantly from those in control tissue. We showed for the first time, the clinical feasibility of this novel suction-based OCE device in evaluating mechanical and structural properties in pathological skin conditions such as scars.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202300314","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acquisition Time for Resting-State HbO/Hb Coupling Measured by Functional Near-Infrared Spectroscopy in Assessing Autism 用功能性近红外光谱法测量静息状态 HbO/Hb 耦合以评估自闭症的采集时间。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-09-04 DOI: 10.1002/jbio.202400150

Fang Lin

引用次数: 0

Noncontact Diffuse Reflectance Spectroscopy of Synovial Fluid Samples for Rapid Identification of Infections 用于快速鉴定感染的滑膜液样本非接触式漫反射光谱仪

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-09-04 DOI: 10.1002/jbio.202400213

Erin E. Drewke, Robert L. Brand, Caroline G. Geels, Hanna K. Jensen, Kevin Wong, Jarret D. Sanders, Narasimhan Rajaram

{"title":"Noncontact Diffuse Reflectance Spectroscopy of Synovial Fluid Samples for Rapid Identification of Infections","authors":"Erin E. Drewke, Robert L. Brand, Caroline G. Geels, Hanna K. Jensen, Kevin Wong, Jarret D. Sanders, Narasimhan Rajaram","doi":"10.1002/jbio.202400213","DOIUrl":"10.1002/jbio.202400213","url":null,"abstract":"<p>Severe joint infections, such as septic arthritis, require rapid diagnostic testing of the synovial fluid aspirated from joints level so that a surgical team can be assembled quickly. We present a diffuse reflectance spectroscopy (DRS) system for noncontact determination of infection. Using a light-tight syringe holder and fiber optic probe, diffusely reflected light from 475 to 655 nm was acquired from 18 patient samples through the wall of a syringe in a noncontact and sterile manner. We determined the reflectance ratios at two different wavelengths—<i>R</i>\u0000 <sub>490</sub>/<i>R</i>\u0000 <sub>600</sub> and <i>R</i>\u0000 <sub>580</sub>/<i>R</i>\u0000 <sub>600</sub> and found statistically significant differences (<i>p</i> < 0.05) in both ratios between the infected and noninfected groups. Critically, the <i>R</i>\u0000 <sub>490</sub>/<i>R</i>\u0000 <sub>600</sub> and <i>R</i>\u0000 <sub>580</sub>/<i>R</i>\u0000 <sub>600</sub> ratios were significantly correlated with clinical biomarkers—the white blood cell (WBC) and red blood cell (RBC) counts, respectively. This study demonstrates the potential of DRS as a rapid diagnostic tool for joint infections.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400213","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Limbus Biomechanical Properties Using Optical Coherence Elastography 利用光学相干弹性成像技术表征韧带生物力学特性

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-09-03 DOI: 10.1002/jbio.202400275

Yubao Zhang, Yue Wang, Xiao Han, Jiahui Luo, Chuanqi Lin, Qin Zhang, Xingdao He

引用次数: 0

The Effect of Repeated Capillary Refill Tests on the Cutaneous Microcirculation 重复毛细血管再充盈试验对皮肤微循环的影响

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-09-03 DOI: 10.1002/jbio.202400098

Frida Meyer, Joakim Henricson, Chris D. Anderson, Daniel B. Wilhelms

{"title":"The Effect of Repeated Capillary Refill Tests on the Cutaneous Microcirculation","authors":"Frida Meyer, Joakim Henricson, Chris D. Anderson, Daniel B. Wilhelms","doi":"10.1002/jbio.202400098","DOIUrl":"10.1002/jbio.202400098","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The capillary refill test (CR test) is often used in emergency care, and the capillary refill time (CR time) is used to assess a patient's circulatory condition. The objective of this study was to investigate if repeated CR tests affect CR time.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Thirteen healthy volunteers had repeated CR tests performed on the sternum, forehead, and fingers. The tests were filmed using polarized reflectance spectroscopy and dedicated software for objective quantification of the CR time.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There were no statistical differences between the first CR test in a series and the following. However, there were statistically significant differences in CR time between the different anatomical sites.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Repeated CR tests, separated by a minimum of 2 min, do not affect CR time in healthy volunteers. The site where the test is performed is of importance for CR time.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142127756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of REFIX Technology on the Remineralization of Artificial Early Enamel Caries Lesion by Laser Speckle Tracking Analysis 通过激光斑点跟踪分析评估 REFIX 技术对人工早期釉质龋损的再矿化作用。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-09-02 DOI: 10.1002/jbio.202400144

Amanda Rafaelly Honório Mandetta, Maria Aparecida de Andrade Moreira Machado, Patrícia Bella Costa, Elaine Marcílio Santos, Marcela Letícia Leal Gonçalves, Ana Paula Taboada Sobral, João Vagner Pereira da Silva, Renato Araujo Prates, Alessandro Melo Deana, Sandra Kalil Bussadori

{"title":"Evaluation of REFIX Technology on the Remineralization of Artificial Early Enamel Caries Lesion by Laser Speckle Tracking Analysis","authors":"Amanda Rafaelly Honório Mandetta, Maria Aparecida de Andrade Moreira Machado, Patrícia Bella Costa, Elaine Marcílio Santos, Marcela Letícia Leal Gonçalves, Ana Paula Taboada Sobral, João Vagner Pereira da Silva, Renato Araujo Prates, Alessandro Melo Deana, Sandra Kalil Bussadori","doi":"10.1002/jbio.202400144","DOIUrl":"10.1002/jbio.202400144","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To assess the effectiveness of the REFIX technology in the remineralization process of initial caries simulated on bovine enamel. The assessment involved the analysis of backscatter intensity, which was determined from laser speckle images.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>Twenty-one bovine teeth were divided into three groups: G1 and G7 were submitted to treatment with the REFIX technology for 1 and 7 days, respectively. The control group was treated with deionized water.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A significant difference in backscatter was found between the carious and sound areas in all groups (<i>p</i> = 0.0038, <i>p</i> < 0.0001, and <i>p</i> = 0.0002 for the control group, G1, and G7, respectively). The intergroup comparison revealed no significant difference among the groups studied.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>REFIX technology did not alter the optical properties of the samples of bovine teeth with simulated initial caries lesions after 1 and 7 days of treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of Raman-Reflectance Combination in Quantifying Liver Steatosis and Fat Droplet Size: Evidence From Monte Carlo Simulations and Phantom Studies 拉曼-反射组合在量化肝脏脂肪变性和脂肪滴大小方面的潜力：来自蒙特卡罗模拟和模型研究的证据。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-09-02 DOI: 10.1002/jbio.202400156

Hao Guo, Vanessa S. Zions, Brent A. Law, Kevin C. Hewitt

引用次数: 0

Shot-Noise Limited Nonlinear Optical Imaging Excited With GHz Femtosecond Pulses and Denoised by Deep-Learning 用 GHz 飞秒脉冲激发并通过深度学习去噪的镜头噪声受限非线性光学成像。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-09-01 DOI: 10.1002/jbio.202400186

Wenlong Wang, Junpeng Wen, Yuke Sheng, Chiyi Wei, Cihang Kong, Yalong Liu, Xiaoming Wei, Zhongmin Yang

{"title":"Shot-Noise Limited Nonlinear Optical Imaging Excited With GHz Femtosecond Pulses and Denoised by Deep-Learning","authors":"Wenlong Wang, Junpeng Wen, Yuke Sheng, Chiyi Wei, Cihang Kong, Yalong Liu, Xiaoming Wei, Zhongmin Yang","doi":"10.1002/jbio.202400186","DOIUrl":"10.1002/jbio.202400186","url":null,"abstract":"<div>\u0000 \u0000 <p>Multiphoton fluorescence microscopy excited with femtosecond pulses at high repetition rates, particularly in the range of 100's MHz to GHz, offers an alternative solution to suppress photoinduced damage to biological samples, for example, photobleaching. Here, we demonstrate the use of a U-Net-based deep-learning algorithm for suppressing the inherent shot noise of the two-photon fluorescence images excited with GHz femtosecond pulses. With the trained denoising neural network, the image quality of the representative two-photon fluorescence images of the biological samples is shown to be significantly improved. Moreover, for input raw images with even SNR reduced to −4.76 dB, the trained denoising network can recover the main image structure from noise floor with acceptable fidelity and spatial resolution. It is anticipated that the combination of GHz femtosecond pulses and deep-learning denoising algorithm can be a promising solution for eliminating the trade-off between photoinduced damage and image quality in nonlinear optical imaging platforms.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0