{"title":"Microcirculatory Dysfunction in Patients With Diabetes Mellitus Detected by a Distributed System of Wearable Laser Doppler Flowmetry Analysers","authors":"Elena Zharkikh, Yulia Loktionova, Andrey Dunaev","doi":"10.1002/jbio.202400297","DOIUrl":"10.1002/jbio.202400297","url":null,"abstract":"<div>\u0000 \u0000 <p>The paper is devoted to the study of perfusion and amplitude-frequency spectra of laser Doppler flowmetry (LDF) signals in patients with diabetes mellitus (DM) in different skin areas of the upper and lower extremities using a distributed system of wearable LDF analysers. LDF measurements were performed in the areas of the fingers, toes, wrists and shins. The mean perfusion values, the amplitudes of blood flow oscillations in endothelial, neurogenic, myogenic, respiratory and cardiac frequency ranges, and the values of nutritive blood flow were analysed. The results revealed a decrease in tissue perfusion and nutritive blood flow in the lower extremities and an increase in these parameters in the upper extremities in patients with DM. A decrease in the amplitudes of endothelial and neurogenic oscillations was observed. The obtained results confirm the possibility of using wearable LDF analysers to detect differences in the blood flow regulation in normal and pathological conditions.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ryan Dimmock, Yilong Zhang, Gibran F. Butt, Saaeha Rauz, Zhihong Huang, Ying Yang
{"title":"Characterizing Biomechanics of Limbal Niche Using Vibrational Optical Coherence Elastography","authors":"Ryan Dimmock, Yilong Zhang, Gibran F. Butt, Saaeha Rauz, Zhihong Huang, Ying Yang","doi":"10.1002/jbio.202400172","DOIUrl":"10.1002/jbio.202400172","url":null,"abstract":"<p>The limbal niche is an adult source of epithelial stem cells which regenerate the cornea epithelium. The architecture and biomechanical properties of the limbus have previously been demonstrated to change due to aging and disease. This study aims to non-destructively and simultaneously quantify these limbal niche properties, along with their age-related changes. A lab-built vibrational optical coherence elastography (OCE) device consisting of a phase-sensitive optical coherence tomography (OCT) with a vibrational stimulator has been used to collect structural images and the depth-resolved elasticity of human corneoscleral tissues (aged 4–96 years old). The limbal palisades of Vogt (POV) were delineated well in OCT images which were validated by histology. The POVs have been spatially mapped with simultaneous elasticity measurements in cross-sections, showing tissue stiffness distributions across the undulations. A significant influence of age on the dimensions of the POVs was explored. The elastic modulus within the limbal niches for the ≥65-year-old group was significantly higher than that of the <65-year-old group.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400172","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexander Ushenko, Natalia Pavlyukovich, Oksana Khukhlina, Olexander Pavlyukovich, Iryna Soltys, Alexander Dubolazov, Yurii Ushenko, Ivan Gordey, Jun Zheng, Zhebo Chen, Lin Bin
{"title":"Blood Plasma Film Multifractal Scanning in COVID-19 Consequences Diagnostics","authors":"Alexander Ushenko, Natalia Pavlyukovich, Oksana Khukhlina, Olexander Pavlyukovich, Iryna Soltys, Alexander Dubolazov, Yurii Ushenko, Ivan Gordey, Jun Zheng, Zhebo Chen, Lin Bin","doi":"10.1002/jbio.202400356","DOIUrl":"10.1002/jbio.202400356","url":null,"abstract":"<div>\u0000 \u0000 <p>A 3D phase scanning method was applied to study blood plasma facies, generating layered polarization maps of the object field. The most sensitive parameters to changes in birefringence distribution were identified. Multifractal analysis using wavelet transforms and fractal dimension spectra provided specific insights into the scale self-similarity of the polarization maps. The multifractal spectra of ellipticity distributions were algorithmically derived, revealing that the third- and fourth-order statistical moments were most sensitive to changes in the supramolecular networks of the facies. These findings were successfully applied to differentiate post-COVID-19 effects with high accuracy.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quantitative Characterization of Zebrafish Caudal Fin Regeneration Based on Mueller Matrix OCT In Vivo","authors":"Zaifan Wang, Ke Li, Hui Chen, Zhifang Li, Wangbiao Li, Hui Lin, Liqin Zheng, Xiaoman Zhang, Shulian Wu","doi":"10.1002/jbio.202400376","DOIUrl":"10.1002/jbio.202400376","url":null,"abstract":"<div>\u0000 \u0000 <p>Zebrafish serves as a valuable model for studying tissue regeneration due to their comprehensive regenerative abilities, particularly in bone tissue. In this study, a Mueller matrix optical coherence tomography (OCT) system was applied to monitor the regenerative processes of zebrafish caudal fins in vivo. The analysis focused on evaluating the thickness of the caudal fin tip and the distribution of internal bone tissue during the regenerative process. Subsequently, the effect of ectoine solution on the regeneration process was observed and discussed. Our findings revealed that the caudal fin blastema did not exhibit phase-induced polarization characteristics in the Mueller matrix OCT images. Statistical analyses indicated that the caudal fins did not fully regenerate to their original state within 21 days. Furthermore, the results suggested that ectoine solution could enhance tissue regeneration. This approach provides a method for quantifying zebrafish caudal fin regeneration and advances observation techniques for biomedical and clinical applications.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optical Transparency Windows in Near-Infrared and Short-Wave Infrared for the Skin, Skull, and Brain: Fluorescence Bioimaging Using PbS Quantum Dots","authors":"Jinghan Qu, Iuliia Golovynska, Jiantao Liu, Junle Qu, Sergii Golovynskyi","doi":"10.1002/jbio.202400171","DOIUrl":"10.1002/jbio.202400171","url":null,"abstract":"<div>\u0000 \u0000 <p>Fluorescence imaging (FI) employing near-infrared (NIR) light within the range of ~750–1350 nm enables biomedical imaging several millimeters beneath the tissue surface. More recent investigations into the short-wave IR (SWIR) transparency windows between ~1550–1870 and 2100–2300 nm highlight their superior capabilities. This research presents a comparison of IR-FI of PbS quantum dots, emitting at 990, 1310, and 1580 nm, through the mouse scalp skin, skull, and brain. The SWIR fluorescence is the most effectively transmitted signal, showing particularly significant enhancement when passing through the skull, which causes high light scattering. For the analysis of the imaging results and light propagation through the organs, their spectra of attenuation, absorption, and scattering coefficients are measured. In view of biomedical imaging, attenuation due to light scattering is a more destructive factor. Hence, the spatial resolution and imaging contrast can be improved by operating in SWIR due to decreased light scattering.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Noninvasive Detection of the Skin Structure and Inversed Retrieval of Chromophore Information Based on Diffuse Reflectance Spectroscopy","authors":"Jinyao Wang, Dong Li, Bin Chen","doi":"10.1002/jbio.202400118","DOIUrl":"10.1002/jbio.202400118","url":null,"abstract":"<div>\u0000 \u0000 <p>The detection of skin's structure lays the foundation for personalized laser surgery of vascular skin disease, which can be noninvasively achieved by diffuse reflectance spectroscopy (DRS). A two-step inverse Monte Carlo radiation method based on DRS under two source-detector separations was proposed to quantify the skin structure, including chromophore concentration (melanin <i>f</i>\u0000 <sub>m</sub> and hemoglobin <i>f</i>\u0000 <sub>b</sub>), epidermal thickness <i>t</i>\u0000 <sub>epi</sub>, average vessel diameter <i>D</i>\u0000 <sub>ves</sub>, depth <i>d</i>\u0000 <sub>pws</sub> and thickness <i>t</i>\u0000 <sub>pws</sub> of the vascular layer for diseased skin. The method fitted the simulated DRS to the measured DRS iteratively, differences between which were described by a specific objective function to amplify blood absorption at 500–600 nm, and <i>D</i>\u0000 <sub>ves</sub>, <i>d</i>\u0000 <sub>pws</sub>, and <i>t</i>\u0000 <sub>pws</sub> were estimated based on <i>f</i>\u0000 <sub>m</sub>, <i>f</i>\u0000 <sub>b</sub>, and <i>t</i>\u0000 <sub>pws</sub> fitted in the first step. The results showed that the two-step method dramatically improve the inversion accuracy with mean errors of <i>f</i>\u0000 <sub>m</sub>, <i>f</i>\u0000 <sub>b</sub>, <i>t</i>\u0000 <sub>pws</sub>, and <i>d</i>\u0000 <sub>pws</sub> less than 5%.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yong Cheng, Shuqing Wang, Fei Chen, Jiahui Liang, Yan Zhang, Lei Zhang, Wangbao Yin, Suotang Jia, Liantuan Xiao
{"title":"A Stand-Off Laser-Induced Breakdown Spectroscopy (LIBS) System for Remote Bacteria Identification","authors":"Yong Cheng, Shuqing Wang, Fei Chen, Jiahui Liang, Yan Zhang, Lei Zhang, Wangbao Yin, Suotang Jia, Liantuan Xiao","doi":"10.1002/jbio.202400332","DOIUrl":"10.1002/jbio.202400332","url":null,"abstract":"<div>\u0000 \u0000 <p>Bacteria are the primary cause of infectious diseases, making rapid and accurate identification crucial for timely pathogen diagnosis and disease control. However, traditional identification techniques such as polymerase chain reaction and loop-mediated isothermal amplification are complex, time-consuming, and pose infection risks. This study explores remote (~3 m) bacterial identification using laser-induced breakdown spectroscopy (LIBS) with a Cassegrain reflective telescope. Principal component analysis (PCA) was employed to reduce the dimensionality of the LIBS spectral data, and the accuracy of support vector machine (SVM) and Random Forest (RF) algorithms was compared. Multiple repeated experiments showed that the RF model achieved a classification accuracy, recall, precision, and <i>F</i>1-score of 99.81%, 99.80%, 99.79%, and 0.9979, respectively, outperforming the SVM model and providing more accurate remote bacterial identification. The method based on laser-induced plasma spectroscopy and machine learning has broad application prospects, supporting noncontact disease diagnosis, improving public health, and advancing medical research and technological development.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anton A. Plekhanov, Nidjat A. Guseynov, Elena B. Kiseleva, Saddam V. Bopkhoev, Arseniy L. Potapov, Ashrf B. I. Ali, Alexander A. Moiseev, Valentina M. Ryabova, Sergey Y. Ivanov, Alexander A. Muraev, Natalia D. Gladkova, Marina A. Sirotkina
{"title":"The Effect of Cryotherapy on Buccal Blood Vessels Evaluated by Optical Coherence Tomography Angiography: A Pilot Study","authors":"Anton A. Plekhanov, Nidjat A. Guseynov, Elena B. Kiseleva, Saddam V. Bopkhoev, Arseniy L. Potapov, Ashrf B. I. Ali, Alexander A. Moiseev, Valentina M. Ryabova, Sergey Y. Ivanov, Alexander A. Muraev, Natalia D. Gladkova, Marina A. Sirotkina","doi":"10.1002/jbio.202400318","DOIUrl":"10.1002/jbio.202400318","url":null,"abstract":"<p>While cryotherapy is one of the traditional ways to reduce postoperative complications in maxillofacial surgery, the cooling degree is not regulated in most cases and the achieved effect is not properly controlled. Therefore, to develop optimal cooling modes, we propose to study the buccal vascular response to cooling, which has not been previously shown. To evaluate the effect of cooling, we analyzed vessel networks using optical coherence tomography angiography (OCT-A). The cheek vessels were OCT-A monitored using cooling by an ice bag/cooling mask. We found the advantages of using a cooling mask over an ice bag consist of a statistically significant decrease in the perfused vessel density (PVD) of the papillary layer at the oral mucosa. The absence of the reticular layer vessel reaction to any type of cooling was noted. We argue for the necessity to develop optimal modes of cryotherapy, which will contribute to blood perfusion reduction and reduction of PVD recovery.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Regulation of Cerebral Lymphatic Drainage in the Transverse Sinus Region of the Mouse Brain","authors":"Zengjun Xie, Zhihui He, Zhen Yuan, Miao Wang, Feifan Zhou","doi":"10.1002/jbio.202400250","DOIUrl":"https://doi.org/10.1002/jbio.202400250","url":null,"abstract":"Cerebral lymphatic drainage is an important pathway for metabolic waste clearance in the brain, which plays a crucial role in the progression of central nervous system diseases. Recent studies have shown that norepinephrine (NE) is involved in the regulation of cerebral lymphatic drainage function, but the modulation mechanism remains unknown. In this study, we confirmed that NE rapidly reduced glymphatic influx and enhanced meningeal lymphatic clearance. Moreover, the transverse sinus (TS) was the vital region of cerebral lymphatic drainage regulation by NE. Further analysis revealed that NE inhibition could simultaneously enhance glymphatic drainage and dorsal meningeal lymphatic drainage, mainly acting on the TS region. This study demonstrated that the cerebral lymphatic drainage system can be regulated by NE, with the TS region serving as the primary modulating site. The findings provide a potential regulatory target for the amelioration of neurological diseases associated with cerebral lymphatic drainage function.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"56 1","pages":"e202400250"},"PeriodicalIF":2.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142269918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sensitivity of Frequency Domain Near Infrared Spectroscopy for Neurovascular Structure Detection in Biotissue Volume: Numerical Modeling Results","authors":"Mariia Belsheva, Larisa Safonova, Alexey Shkarubo","doi":"10.1002/jbio.202400291","DOIUrl":"https://doi.org/10.1002/jbio.202400291","url":null,"abstract":"Through numerical modeling, it has been determined that near infrared spectroscopy with a frequency domain approach can detect neurovascular structures with diameters from 0.5 mm at source‐detector distances of 5–8 mm, depending on optical parameters and technical implementation of the method. Among the five classical machine learning methods considered, quadratic discriminant analysis is the most effective for detection. Furthermore, it has been demonstrated that the use of a photomultiplier tube and the registration of both amplitude and phase signal components exhibit the highest sensitivity. Spectroscopy can rival modern ultrasound for detecting arterial vessels. A cross‐shaped probe configuration improves sensitivity, and the ratio of reduced scattering coefficient values at different wavelengths is informative for blood‐filled vessel detection. These findings are consistent with and significantly extend previous experimental in vivo and in situ studies and could be valuable for intraoperative diagnostic tasks, particularly in neurosurgery.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"45 1","pages":"e202400291"},"PeriodicalIF":2.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}