Journal of Biophotonics最新文献

{"title":"Corundum Aggregates With Microplastics Revealed by Raman Scattering and Fluorescence Study of Children's Urine in Hospital Environment","authors":"Dmitri V. Voronine,&nbsp;Beder Nourachi,&nbsp;Tien Dao,&nbsp;Humberto R. Gutierrez,&nbsp;Raymond Heromin,&nbsp;JoAnn DeRosa,&nbsp;Antonika Ford,&nbsp;Steven Bruzek,&nbsp;Vera Ignjatovic,&nbsp;William Schleif,&nbsp;Christopher W. Snyder","doi":"10.1002/jbio.70357","DOIUrl":"10.1002/jbio.70357","url":null,"abstract":"<div>\u0000 \u0000 <p>Microplastics (MPs) have been of growing concern in recent years due to their potential adverse health effects and their role as carriers of hazardous substances. Various spectroscopic methods have been developed for the detection of MPs in human samples. However, the interaction of MPs with other environmental pollutants such as corundum dust (CD) has been less explored. This pilot study investigated the correlation between MPs and CD contamination in the urine of pediatric hospital patients using simultaneous Raman spectroscopy and fluorescence imaging. CD particles (CDPs) were detected in both patient urine samples and procedural controls from the pediatric hospital environment with high levels of CD/MP aggregates. In addition, we identified the association of certain MP types with CDPs, supporting the hypothesis that CD may act as a carrier of or scaffold for MPs. Our results suggest that CD might have a potential adverse health effect as a carrier of MPs, inspiring further research into CD pollution in humans and the environment.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148932263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoacoustic Imaging Reveals Early Hemodynamic Changes in Diabetic Nephropathy: Hemoglobin Rise Prior to Oxygen Saturation Drop","authors":"Hong Wang,&nbsp;Jiazhi Cao,&nbsp;Jie Yang,&nbsp;Xijing Yang,&nbsp;Wenwu Ling","doi":"10.1002/jbio.70355","DOIUrl":"10.1002/jbio.70355","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study employed photoacoustic imaging (PAI) to dynamically monitor renal oxygen saturation (sO₂) and hemoglobin concentration (HbT) in a spontaneous Type 2 diabetes model (<i>db/db</i> mice) for early detection of diabetic nephropathy (DN).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p><i>db/db</i> mice with fasting blood glucose (FBG) levels above 11.1 mmol/L (the model group) and C57BL/6 mice (the control group) were assessed at 8, 16, and 32 weeks of age. Evaluations included physiology, biochemistry, histology, and PAI.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Histology confirmed progressive renal injury in <i>db/db</i> mice from 16 weeks. PAI quantification revealed a significant increase in HbT in <i>db/db</i> mice as early as 8 weeks, prior to a significant sO₂ decrease observed at 16 weeks.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The rise in renal HbT precedes the fall in sO₂, identifying it as an early hemodynamic biomarker. PAI demonstrates significant potential for the noninvasive early diagnosis of DN.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148914666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polarization Biophotonics of Hidden Multifractal Structures in Soft Matter Layers for Biomedical Diagnostics","authors":"Yuriy Ushenko,&nbsp;Dou Weidong,&nbsp;Olexander Ushenko,&nbsp;Olexander Dubolazov,&nbsp;Irina Soltys,&nbsp;Mykhaylo Gorsky,&nbsp;Oleg Wanchuliak,&nbsp;Vitaliy Rozhko,&nbsp;Godovanets Oksana,&nbsp;Olexander Olar,&nbsp;Yuriy Tomka,&nbsp;Vladyslava Sholota,&nbsp;Diana Olar,&nbsp;Vasyl Prysyazhyuk","doi":"10.1002/jbio.70349","DOIUrl":"10.1002/jbio.70349","url":null,"abstract":"<div>\u0000 \u0000 <p>This study develops and substantiates a polarization-interferometric Jones-matrix approach for revealing hidden multifractal optical anisotropy in dehydrated biological soft-matter films. The method integrates Mach–Zehnder interferometry, digital holographic reconstruction of complex amplitudes, phase-selective speckle-field analysis, and statistical–multifractal processing of Jones-matrix images. Unlike intensity-based Stokes polarimetry, it provides amplitude and phase information and enables selective analysis of the coherent ballistic or weakly scattered polarization-preserving field component. Analytical relationships between real and imaginary Jones-matrix components and linear/circular birefringence mechanisms establish their interpretation as markers of spatial heterogeneity, orientational consistency, and phase organization of supramolecular polycrystalline networks. Using bile films, phase-selective mapping revealed distinct anisotropy manifestations in dendritic and spherulitic structures. Higher-order statistical moments and multifractal spectra described coordinate- and scale-dependent transformations. Primary validation on bile, whole blood, and dental canal irrigation films showed comparable integral-field accuracy but higher Jones-matrix informativeness after minimally scattered component isolation, supporting label-free biomedical diagnostics requiring further clinical validation.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phasor-Based Spatio-Spectral Segmentation for Hyperspectral Fluorescence Microscopy","authors":"Maciej Barna,&nbsp;Grazyna Palczewska,&nbsp;Grzegorz Soboń,&nbsp;Krzysztof Palczewski,&nbsp;Jakub Bogusławski","doi":"10.1002/jbio.70351","DOIUrl":"10.1002/jbio.70351","url":null,"abstract":"<p>Fluorescence microscopy is essential for visualizing cellular and tissue structures. When combined with a hyperspectral modality, it enables the acquisition of emission spectra that provide insight into the composition of fluorophores. These spectra can be used for fluorophore-based image segmentation; however, accurate spectral separation becomes challenging when fluorophore emission spectra overlap and mix within individual pixels. We present a spatially-corrected density peak clustering (SC-DPC) method that combines phasor transformation with histogram-based density-peak detection and additional spatial correction to achieve accurate and fast clustering. SC-DPC effectively handles large datasets and clusters that are not separable linearly in phasor space while maintaining high clustering accuracy and computational efficiency. Results with synthetic data and experimental multiphoton microscopic data from the mouse retina demonstrate that SC-DPC provides a robust, high-performance approach for hyperspectral fluorescence data segmentation.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13541396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"fNIRS-Based Network Signatures of Sensory-Primed Bilateral Arm Training in Subacute Stroke Recovery","authors":"Wenhao Li,&nbsp;Bing Wang,&nbsp;Qiang Zhang,&nbsp;Ran Wei,&nbsp;Congcong Huo","doi":"10.1002/jbio.70353","DOIUrl":"https://doi.org/10.1002/jbio.70353","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Significance</h3>\u0000 \u0000 <p>This study leverages functional near-infrared spectroscopy (fNIRS) to elucidate optically derived cortical network signatures underlying combined sensory–motor interventions in stroke rehabilitation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To characterize the synergistic effects of peripheral electrical stimulation (PES) and bilateral arm training (BAT) on cortical hemodynamics and functional network architecture using fNIRS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Approach</h3>\u0000 \u0000 <p>fNIRS signals were acquired from 24 subacute stroke patients. Wavelet amplitude and wavelet phase coherence were employed to quantify task-related hemodynamic activation and interregional synchronization within the 0.01–0.08 Hz frequency band.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Compared with BAT alone, the PES-BAT paradigm elicited augmented hemodynamic activation in ipsilesional prefrontal and motor regions. The combined intervention further enhanced phase synchronization across distributed cortical networks and preserved interhemispheric coherence, counteracting maladaptive contralesional dominance.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The fNIRS-based wavelet metrics effectively detect intervention-dependent modulation of cortical hemodynamics and network organization, highlighting the value of optical neuroimaging in assessing neuroplasticity during stroke rehabilitation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autofluorescence Imaging and Methylene Blue-Mediated Photodynamic Therapy for Early Detection and Management of Subclinical Actinic Cheilitis","authors":"Juliana Cristina Oliverio de Araújo,&nbsp;Og Fray Junior,&nbsp;Hermano Camelo Paiva,&nbsp;Alyne Simões Gonçalves,&nbsp;Martha Simões Ribeiro","doi":"10.1002/jbio.70346","DOIUrl":"https://doi.org/10.1002/jbio.70346","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Optical autofluorescence imaging is a noninvasive adjunct for detecting potentially malignant oral disorders, such as actinic cheilitis (AC). This study evaluated its use for early detection and for monitoring tissue response following methylene blue-mediated photodynamic therapy (MB-PDT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Lip autofluorescence was assessed using a handheld device (405 ± 20 nm). Areas with altered fluorescence underwent biopsy for confirmation. Patients with AC received five MB-PDT sessions (660 nm, 100 mW, 120 s/point) after topical 1% MB. Grayscale intensity was quantified before treatment and at 30 days.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of 25 screened individuals, 19 had confirmed AC, and 17 completed follow-up. Lesions showed higher fluorescence intensity than healthy tissue. After MB-PDT, mean intensity and variability significantly decreased, indicating tissue response. Smokers showed a smaller reduction.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Autofluorescence imaging enables early detection and noninvasive monitoring of AC. Combined with MB-PDT, it represents a practical strategy for managing high-risk patients and supporting early intervention.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70346","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Does the Photoplethysmography Add to Laser Doppler Flowmetry During the Brachial Artery Occlusion Test?","authors":"Vasily Karpov,&nbsp;Alexey Glazkov,&nbsp;Denis Lapitan,&nbsp;Aleksandra Ivlieva,&nbsp;Dariya Selivanova,&nbsp;Dmitry Rogatkin","doi":"10.1002/jbio.70347","DOIUrl":"https://doi.org/10.1002/jbio.70347","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, the difference in behavior of the raw photoplethysmography (PPG) signal and the laser Doppler flowmetry (LDF) signal reflecting hemodynamics in the skin of upper extremities at brachial arterial occlusion is recorded and discussed. It is shown that at various locations on the hand, the raw PPG signal provides richer and previously little-known information on the redistribution of blood between the arterial, microcirculatory, and venous beds, while the conventional blood flow (BF) parameter recorded by LDF technique is less sensitive to it and largely does not capture this redistribution.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vivo Monitoring of Pressure Ulcer Model Development and Recoverability Under Multimodal Parameters Within a Unified Field of View","authors":"Qimeng Liu,&nbsp;Yixin Wang,&nbsp;Mu Chen,&nbsp;Shihao Tang,&nbsp;Yamin Yang,&nbsp;Xiaofei Jin,&nbsp;Jie Dai,&nbsp;Yameng Zhang,&nbsp;Weitao Li","doi":"10.1002/jbio.70348","DOIUrl":"https://doi.org/10.1002/jbio.70348","url":null,"abstract":"<div>\u0000 \u0000 <p>This study utilizes an integrated laser speckle (LSCI) and intrinsic optical signal imaging (IOSI) system to evaluate microvascular dynamics in murine pressure ulcer (PU) models. We compared a single prolonged compression (6 h) against repeated compressions (1.5 h/day for 7 days) using an enhanced spatiotemporal gradient analysis of LSCI data. In the repeated compression model, gradient analysis revealed a progressive perfusion decline by Day 4. By Day 7, perfusion gradients indicated near-complete flow loss, corresponding to severe hypoxia detected by IOSI, signifying sustained microvascular impairment. Conversely, the single compression model exhibited reversible alterations; LSCI gradients showed initial disruption followed by gradual recovery, with perfusion nearing baseline by Day 7. The LSCI-IOSI platform, emphasizing advanced gradient processing, systematically delineates microcirculatory compromise. This approach highlights the critical role of repeated ischemia–reperfusion in driving persistent dysfunction and demonstrates the utility of quantitative optical imaging for early detection and targeted intervention in PU progression.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep Learning-Based Inclusion Boundary Identification Using Wave Propagation in Optical Coherence Elastography","authors":"Guangyu Zhang,&nbsp;Jinpeng Liao,&nbsp;Zhengshuyi Feng,&nbsp;Alison M. Layton,&nbsp;Chunhui Li,&nbsp;Zhihong Huang","doi":"10.1002/jbio.70345","DOIUrl":"10.1002/jbio.70345","url":null,"abstract":"<p>Elastography is widely used to estimate tissue mechanical properties based on wave propagation characteristics. However, a major limitation is that elastography maps often fail to accurately delineate internal boundaries within heterogeneous tissues. To address this issue, we propose a novel deep learning–based framework, termed Boundary Detection Network (BDNet). Depth-resolved phase images capturing wave propagation are acquired using Optical Coherence Elastography (OCE). The proposed method is first evaluated on agar phantoms. Experimental results show that all evaluated models achieve comparable performance on single-boundary samples, with a mean absolute error (MAE) of 2 pixels. For dual-boundary phantoms, BDNet achieves an overall MAE of 4.33 pixels. Furthermore, on a human acne dataset, the proposed method provides the best performance, with an MAE of 19.11 pixels. The proposed BDNet demonstrates high accuracy and computational efficiency, providing a promising tool for assisting abnormal tissue morphology identification in clinical elastography applications.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13500869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148809727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Assessment of Composition-Dependent Mechanical Properties of Atherosclerotic Plaques by Photoacoustic Elasticity and Viscosity Imaging","authors":"Ao Jiang,&nbsp;Yuxuan Liang,&nbsp;Yiting Tan,&nbsp;Heng Zhang,&nbsp;Pingping Wang","doi":"10.1002/jbio.70342","DOIUrl":"https://doi.org/10.1002/jbio.70342","url":null,"abstract":"<div>\u0000 \u0000 <p>Evaluating the mechanical properties of atherosclerotic plaques is crucial for precisely identifying rupture-prone lesions, as different plaque constituents dictate local biomechanical instability and directly inform targeted therapeutic intervention. In this study, we further achieved quantitative photoacoustic elasticity imaging (PAEI) and photoacoustic viscosity imaging (PAVI) by analyzing the rise time of the thermoelastic displacement and by measuring the phase delay of the photoacoustic signal relative to the laser pulse, respectively. Experiments on mice aortas have shown that PAVI is more sensitive to the changes in lipid core area during lipid plaque stage, whereas PAEI is more effective at detecting changes in fibrous cap thickness during fibrous plaque stage. Furthermore, PAEI and PAVI can effectively distinguish lipid plaques and fibrous plaques from normal vascular tissue. All results indicate that the PAEI–PAVI imaging system enables label-free, high-resolution visualization of the component-based biomechanical properties of atherosclerotic plaques, facilitating early detection and rupture risk assessment.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}