Journal of Biophotonics最新文献_第3页

A Miniaturized In Vivo Fluorescence Microscopy Method for Monitoring Circulating Tumor Cells in Freely Moving Animals 一种用于监测自由运动动物循环肿瘤细胞的小型体内荧光显微镜方法。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-12-23 DOI: 10.1002/jbio.202400496

Bingchen Yu, Yuxi Zhu, Fan Zhang, Da Sun, Rui Xu, Chenzheng Wang, Kai Pang

{"title":"A Miniaturized In Vivo Fluorescence Microscopy Method for Monitoring Circulating Tumor Cells in Freely Moving Animals","authors":"Bingchen Yu, Yuxi Zhu, Fan Zhang, Da Sun, Rui Xu, Chenzheng Wang, Kai Pang","doi":"10.1002/jbio.202400496","DOIUrl":"10.1002/jbio.202400496","url":null,"abstract":"<div>\u0000 \u0000 <p>Metastasis is the leading cause of death in tumor patients, with circulating tumor cells (CTCs) serving as key biomarkers for tumor progression, metastasis, and recurrence. CTC quantity is closely linked to tumor dynamics, which are influenced by biological rhythms. Studying CTC distribution under various physiological conditions provides insights into metastasis mechanisms. However, due to the low abundance of CTCs, detection accuracy is limited, especially with small blood samples, making continuous data collection challenging. To address this, we developed a dual-channel miniaturized in vivo fluorescence microscopy system for real-time monitoring of CTCs in experimental animals. This system, which can be fixed to the head or back, enables dynamic, quantitative analysis of CTCs in the circulatory system. It offers a valuable tool for investigating tumor metastasis rhythms, drug evaluation, and prognostic assessment in freely moving animals, advancing research in metastasis mechanisms and cancer treatment.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Visible–Near Infrared Projected Multispectral Imaging System for Intraoperative Breast Tumor Margin Assessment 术中乳腺肿瘤边缘评估的可见-近红外投影多光谱成像系统的研制。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-12-16 DOI: 10.1002/jbio.202400370

Hao Wang, Shuyu Chen, Sisi Guo, Jing Gao, Xudong Zhou, Qian Chen, Houpu Yang, Fei Xie, Shu Wang, Kun Gao, Lihui Liu, Ruoyu Zhang, Jianfeng Wang

{"title":"Development of a Visible–Near Infrared Projected Multispectral Imaging System for Intraoperative Breast Tumor Margin Assessment","authors":"Hao Wang, Shuyu Chen, Sisi Guo, Jing Gao, Xudong Zhou, Qian Chen, Houpu Yang, Fei Xie, Shu Wang, Kun Gao, Lihui Liu, Ruoyu Zhang, Jianfeng Wang","doi":"10.1002/jbio.202400370","DOIUrl":"10.1002/jbio.202400370","url":null,"abstract":"<div>\u0000 \u0000 <p>A visible–near infrared (VIS–NIR) projected multispectral imaging (Proj-MSI) system consisting of an MSI subsystem and a compact projector for intraoperative breast tumor margin assessment was developed. MSI with an average spectral resolution of 24 nm was realized through sequential illumination of 26 sets of VIS–NIR light-emitting diodes and synchronized single NIR-sensitive camera image capture. Rapid (~1 min) tumor margin delineation revealed significantly (<i>p</i> < 0.01, Student's <i>t</i>-test) reduced reflectivity associated with breast tumor. Speeded-up robust features algorithm-based field of view calibration allowed the MSI identified tumor margins to be projected directly onto the breast-conserving surgery (BCS) surgical cavity with a projection error of < 1 mm. Besides, the projected tumor margin boundaries were outlined using Indian ink to simulate breast tumor removal, demonstrating the potential of the Proj-MSI system developed for intraoperative tumor margin assessment during BCS.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulsed Red Photobiomodulation Boosts the Inhibition of Oxytocin-Induced Primary Dysmenorrhea in Mice by Suppressing Oxidative Stress and Inflammation 脉冲红色光生物调节通过抑制氧化应激和炎症增强对小鼠催产素诱导的原发性痛经的抑制作用。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-12-15 DOI: 10.1002/jbio.202400398

Qiqi Fu, Jiali Yang, Hui Jiang, Yi Ren, Longfei Huo, Muqing Liu

{"title":"Pulsed Red Photobiomodulation Boosts the Inhibition of Oxytocin-Induced Primary Dysmenorrhea in Mice by Suppressing Oxidative Stress and Inflammation","authors":"Qiqi Fu, Jiali Yang, Hui Jiang, Yi Ren, Longfei Huo, Muqing Liu","doi":"10.1002/jbio.202400398","DOIUrl":"10.1002/jbio.202400398","url":null,"abstract":"<div>\u0000 \u0000 <p>Increasing evidence has underscored the pivotal role of red photobiomodulation (R-PBM) in analgesic and anti-inflammatory processes; nonetheless, research concerning the effects of pulsed wave on primary dysmenorrhea (PD) remains sparse. This study found that pulsed R-PBM significantly diminished pain responses and levels of PGF<sub>2α</sub>/PGE<sub>2</sub>, mitigated uterine swelling, augmented antioxidant capacity, and lowered MDA concentrations, which outperformed continuous wave at the same average irradiance. Furthermore, PW treatment substantially reduced ROS levels and enhanced cell viability in PGF<sub>2α</sub> induced HUSM cells. NOS levels, especially iNOS, were markedly diminished in the uteri of PD mice, accompanied by significant alterations in inflammation-related genes (<i>Jun</i>, <i>Fos</i>, <i>IL1rn</i>, <i>IL17b</i>) and protein levels, along with pronounced downregulation of calcium ion concentrations after pulsed R-PBM intervention. These findings indicated that pulsed R-PBM may mitigate pain by modulating ROS and NO/NOS, mediated oxidative stress and inflammatory responses. Consequently, pulsed R-PBM emerges as a promising therapeutic strategy for PD.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Theranostic-Guided UV-A Light Corneal Wavefront Photo-Reshaping for Presbyopia Correction: A Preclinical Study 治疗导向UV-A光角膜波前整形治疗老花眼的临床前研究。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-12-12 DOI: 10.1002/jbio.202400462

Marco Lombardo, Giuseppe Massimo Bernava, Sebastiano Serrao, Giuseppe Lombardo

{"title":"Theranostic-Guided UV-A Light Corneal Wavefront Photo-Reshaping for Presbyopia Correction: A Preclinical Study","authors":"Marco Lombardo, Giuseppe Massimo Bernava, Sebastiano Serrao, Giuseppe Lombardo","doi":"10.1002/jbio.202400462","DOIUrl":"10.1002/jbio.202400462","url":null,"abstract":"<p>This study investigated the effect of a theranostic-guided UV-A light corneal photo-reshaping technique on corneal elevation and wavefront aberration (WA) in human donor eyes. A specialized platform, combining UV-A light with corneal iontophoresis for controlled, patterned, riboflavin delivery, was used for both distribution assessment and concentration-driven photopolymerization of corneal proteins. In all cases, a consistent riboflavin concentration gradient, with lower levels in the central prepupillary zone, was recorded. Corneal topography and WA measurements showed significant corneal steepening and smooth wavefront shaping, respectively, with a delay in the central 2.0 mm of the WA and advancement in the surrounding zone, as well as a 50% reduction in corneal spherical aberration over a 5.0 mm pupil size. Notably, the corneal optical quality, measured via modulation transfer function (MTF), remained stable. This incision-free approach demonstrated the potential to extend focal range without compromising distance vision, presenting a new solution for presbyopia correction.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400462","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Wavelength LEDs Induce Reactive Oxygen Species and Nitric Oxide That Inhibit the Production of Dihydrotestosterone by 5-α Reductase 双波长led诱导活性氧和一氧化氮抑制5-α还原酶产生双氢睾酮。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-12-12 DOI: 10.1002/jbio.202400388

Jacob Kocher, Nicole Jandick, Derry Spragion, P. Joseph DeSena Jr, T. Matthew Womble, Katelyn Crizer, Nathan Stasko

{"title":"Dual Wavelength LEDs Induce Reactive Oxygen Species and Nitric Oxide That Inhibit the Production of Dihydrotestosterone by 5-α Reductase","authors":"Jacob Kocher, Nicole Jandick, Derry Spragion, P. Joseph DeSena Jr, T. Matthew Womble, Katelyn Crizer, Nathan Stasko","doi":"10.1002/jbio.202400388","DOIUrl":"10.1002/jbio.202400388","url":null,"abstract":"<div>\u0000 \u0000 <p>Androgenetic alopecia (AGA) causes balding in approximately 50% of adults. One primary cause of AGA is synthesis of dihydrotestosterone from testosterone by 5-α reductase. Systemic pharmaceutical interventions have potentially serious side effects, necessitating development of localized interventions. One such approach is administration of red light via low level light therapy (LLLT), which has promising clinical data. However, the LLLT mechanism of action remains unclear. We investigated the ability of LLLT to stimulate nitric oxide (NO) and the role of NO in inhibition of DHT synthesis. Our results show that red and red-orange light induce NO release in a cell-free platform. In A549 and HEK293T cells, we demonstrate 620 and 660 nm LED-emitted light stimulates the production of NO, reactive oxygen species (ROS), and decreases DHT synthesis. These results provide a plausible mechanism of action for LLLT employing LED-emitted red and red-orange wavelengths of light to treat AGA.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental Study on the Process and Performance of Pre-Dressing-Assisted Laser Joining of Vascular Tissues 预敷料辅助激光血管组织连接工艺及性能的实验研究。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-12-09 DOI: 10.1002/jbio.202400480

Jun Huang, Yanyu Li, Mintao Yan, Tongyu Xu, Kehong Wang

引用次数: 0

Label-Free Surface-Enhanced Raman Spectroscopy Detection of Amyloid Beta on Silver Nanostructured Substrates for Alzheimer's Diagnosis 无标记表面增强拉曼光谱检测银纳米结构底物上的淀粉样蛋白用于阿尔茨海默病的诊断。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-12-04 DOI: 10.1002/jbio.202400314

Navami Sunil, Ashma Shikkandar, Baby Roselin Rajan Durai, Rajesh Unnathpadi, Veintramuthu Sankar, Biji Pullithadathil

{"title":"Label-Free Surface-Enhanced Raman Spectroscopy Detection of Amyloid Beta on Silver Nanostructured Substrates for Alzheimer's Diagnosis","authors":"Navami Sunil, Ashma Shikkandar, Baby Roselin Rajan Durai, Rajesh Unnathpadi, Veintramuthu Sankar, Biji Pullithadathil","doi":"10.1002/jbio.202400314","DOIUrl":"10.1002/jbio.202400314","url":null,"abstract":"<div>\u0000 \u0000 <p>Alzheimer's disease (AD) is a public health concern, for which an early diagnosis is essential. Biomass-derived carbon fibres with surface-decorated silver nanoparticles (Ag@CFs) have been utilized as surface enhanced raman spectroscopy (SERS) sensor platform for the detection of the amyloid beta Aβ (25–35) for the pre-diagnosis of Alzheimer's disease. Structural and morphological characterizations confirmed the distribution of plasmonic silver nanoparticles over the surface of carbon fibres. The SERS sensor performance of Ag@CFs was evaluated using rhodamine 6G, which showed an enhancement of the order of 10<sup>6</sup> which proved the effectiveness of the developed SERS sensor towards trace level detection of analyte. A range of amyloid beta concentrations, from 100uM to 10pM, have been analyzed as a proof-of concept for this study, showcasing the efficacy of the Ag@CFs based SERS sensor to detect trace level concentrations of amyloid beta, even as low as 10 pM. This investigation is a promising development in the field of AD diagnostics since it may turn out to be a non-invasive, economical and early diagnostic tool.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the Impact of Chronical Prenatal Alcohol Exposure on Fetal Vascular Development Across Pregnancy Stages Using Photoacoustic Tomography 利用光声断层扫描研究慢性产前酒精暴露对妊娠期胎儿血管发育的影响

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-12-03 DOI: 10.1002/jbio.202400410

Hao Yang, Md Farhan Tanvir, Huabei Jiang

引用次数: 0

1550 Nm Optical Coherence Tomography for In Vivo Deep Brain Cerebral Blood Flow Imaging 用于体内脑深部脑血流成像的 1550 牛米光学相干断层扫描。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-11-28 DOI: 10.1002/jbio.202400306

Wei Chen, Xiangsen Guo, Junxiong Zhou, Yanjun Zhang, Yuerong Bao, Yongchao Wang, Fen Yang, Jianbo Tang

引用次数: 0

Clinical Effect Analysis and Prognostic Factors of Photodynamic Therapy for Cervical Precancerous Lesions 光动力疗法治疗宫颈癌前病变的临床效果分析和预后因素

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-11-28 DOI: 10.1002/jbio.202400454

Hong Zhu, Xiangrui Guo, Xu Li, Mengran Chang, Zhongyin Zhang, Junjun Dai

{"title":"Clinical Effect Analysis and Prognostic Factors of Photodynamic Therapy for Cervical Precancerous Lesions","authors":"Hong Zhu, Xiangrui Guo, Xu Li, Mengran Chang, Zhongyin Zhang, Junjun Dai","doi":"10.1002/jbio.202400454","DOIUrl":"10.1002/jbio.202400454","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To evaluate the therapeutic effect of photodynamic therapy (PDT) on cervical precancerous lesions and prognostic factors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>173 patients diagnosed with cervical precancerous lesions and treated with PDT were included. Univariate analysis was performed on human papillomavirus (HPV) infection, patient age, number of treatments and number of medications before and after treatment, to evaluate the efficacy, adverse reactions and patient satisfaction.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>112 patients (64.5%) showed HPV clearance afrer PDT. Age ≥ 40 years was positively correlated with poor prognosis and number of treatments ≥ 6 was negatively correlated with poor prognosis. The satisfaction rate reached 94.79%, with 6 patients experiencing tolerable pain and no treatment discontinuations due to adverse reactions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>PDT is effective and safe for treating cervical precancerous lesions, with patient age and treatment frequency impacting outcomes. High patient satisfaction and tolerable adverse reactions support the application of PDT in clinical practice.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142741868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0