一种临床药物作为三光子荧光探针用于小鼠肾脏的体内显微成像。

Weihang Geng, Qiming Xia, Mubin He, Yi Wang, Zheng Zheng, Haiyan Qin, Yalun Wang, Jian Feng, Jun Qian
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引用次数: 0

摘要

三光子荧光(3PF)显微镜在生物学研究和临床应用中遇到了重大挑战,主要是由于高性能探针的可用性有限。我们从中药黄连中提取的一种临床常用药物盐酸小檗碱(berberine hydrochloride, BH),探索其优异的3PF特性,走了一条捷径。利用其肾脏代谢特性,我们采用BH对小鼠肾脏进行体内3PF显微成像。该方法能够对肾小管进行高分辨率的结构观察,有助于基于肾小管形态学诊断药物性急性肾损伤(AKI)。3PF显微镜对肾脏生理和病理诊断的分析能力表明其在未来临床应用的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Clinical Drug as the Three-Photon Fluorescence Probe for In Vivo Microscopic Imaging of Mouse Kidney.

Three-photon fluorescence (3PF) microscopy encounters significant challenges in biological research and clinical applications, primarily due to the limited availability of high-performance probes. We took a shortcut by exploring the excellent 3PF property of berberine hydrochloride (BH), a clinically utilized drug derived from the traditional Chinese medicine, Coptis. Capitalizing on its renal metabolism characteristics, we employed BH for in vivo 3PF microscopic imaging of the mouse kidney. This approach enabled high-resolution structural observation of renal tubules and facilitated the diagnosis of drug-induced acute kidney injury (AKI) based on renal tubule morphology. The analytical capabilities of 3PF microscopy for renal physiology and pathological diagnosis suggest its potential for future clinical applications.

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