Simone Sleep, Deanne H. Hryciw, Laurence J. Walsh, Eliza Ranjit, Nifty Tomy, Praveen R. Arany, Roy George
{"title":"多种近红外 LED（700、850 和 980 纳米）CW-PBM 对 MG63 骨母细胞线粒体呼吸和基因表达的影响。","authors":"Simone Sleep, Deanne H. Hryciw, Laurence J. Walsh, Eliza Ranjit, Nifty Tomy, Praveen R. Arany, Roy George","doi":"10.1002/jbio.70015","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background and Aim</h3>\n \n <p>This study evaluated mitochondrial and osteogenic activity in MG-63 pre-osteoblastic cells after photobiomodulation (PBM) using multiple near-infrared LED sources (Nuralyte) emitting wavelengths from 700 to 1100 nm.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>MG-63 cells were irradiated daily for 3, 5, or 7 days with energy densities of 5.3 J/cm<sup>2</sup> (30 s, optimal dose) and 10.6 J/cm<sup>2</sup> (60 s, high dose). Mitochondrial function was assessed using the XF Seahorse analyzer, and gene expression of osteogenic markers was analyzed.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Maximal mitochondrial oxygen consumption rate (OCR) significantly decreased at the optimal dose but increased at the high dose (<i>p</i> < 0.001) in 5-day irradiated cultures. Upregulation of osteogenic markers (OCN, OPN, BMP-2, COL-1, RUNX2) occurred after 3–5 consecutive days of irradiation, with greater activation at the optimal dose.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>MG-63 cells respond to PBM using MNI-LEDs (700, 850, 980 nm) by modulating mitochondrial respiration and boosting bone-related gene expression in a dose- and time-dependent manner.</p>\n </section>\n </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70015","citationCount":"0","resultStr":"{\"title\":\"Effects of Multiple Near-Infrared LEDs (700, 850, and 980 nm) CW-PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts\",\"authors\":\"Simone Sleep, Deanne H. Hryciw, Laurence J. Walsh, Eliza Ranjit, Nifty Tomy, Praveen R. Arany, Roy George\",\"doi\":\"10.1002/jbio.70015\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background and Aim</h3>\\n \\n <p>This study evaluated mitochondrial and osteogenic activity in MG-63 pre-osteoblastic cells after photobiomodulation (PBM) using multiple near-infrared LED sources (Nuralyte) emitting wavelengths from 700 to 1100 nm.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Materials and Methods</h3>\\n \\n <p>MG-63 cells were irradiated daily for 3, 5, or 7 days with energy densities of 5.3 J/cm<sup>2</sup> (30 s, optimal dose) and 10.6 J/cm<sup>2</sup> (60 s, high dose). Mitochondrial function was assessed using the XF Seahorse analyzer, and gene expression of osteogenic markers was analyzed.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Maximal mitochondrial oxygen consumption rate (OCR) significantly decreased at the optimal dose but increased at the high dose (<i>p</i> < 0.001) in 5-day irradiated cultures. Upregulation of osteogenic markers (OCN, OPN, BMP-2, COL-1, RUNX2) occurred after 3–5 consecutive days of irradiation, with greater activation at the optimal dose.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>MG-63 cells respond to PBM using MNI-LEDs (700, 850, 980 nm) by modulating mitochondrial respiration and boosting bone-related gene expression in a dose- and time-dependent manner.</p>\\n </section>\\n </div>\",\"PeriodicalId\":184,\"journal\":{\"name\":\"Journal of Biophotonics\",\"volume\":\"18 8\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-04-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70015\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biophotonics\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jbio.70015\",\"RegionNum\":3,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biophotonics","FirstCategoryId":"101","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jbio.70015","RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Effects of Multiple Near-Infrared LEDs (700, 850, and 980 nm) CW-PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts
Background and Aim
This study evaluated mitochondrial and osteogenic activity in MG-63 pre-osteoblastic cells after photobiomodulation (PBM) using multiple near-infrared LED sources (Nuralyte) emitting wavelengths from 700 to 1100 nm.
Materials and Methods
MG-63 cells were irradiated daily for 3, 5, or 7 days with energy densities of 5.3 J/cm2 (30 s, optimal dose) and 10.6 J/cm2 (60 s, high dose). Mitochondrial function was assessed using the XF Seahorse analyzer, and gene expression of osteogenic markers was analyzed.
Results
Maximal mitochondrial oxygen consumption rate (OCR) significantly decreased at the optimal dose but increased at the high dose (p < 0.001) in 5-day irradiated cultures. Upregulation of osteogenic markers (OCN, OPN, BMP-2, COL-1, RUNX2) occurred after 3–5 consecutive days of irradiation, with greater activation at the optimal dose.
Conclusion
MG-63 cells respond to PBM using MNI-LEDs (700, 850, 980 nm) by modulating mitochondrial respiration and boosting bone-related gene expression in a dose- and time-dependent manner.
期刊介绍：
The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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