Marine Drugs最新文献_第4页

Selenium-Chondroitin Sulfate Nanoparticles Inhibit Angiogenesis by Regulating the VEGFR2-Mediated PI3K/Akt Pathway.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-01-02 DOI: 10.3390/md23010022

Xia Zheng, Xiaofei Liu, Zhuo Wang, Rui Li, Qiaoli Zhao, Bingbing Song, Kit-Leong Cheong, Jianping Chen, Saiyi Zhong

{"title":"Selenium-Chondroitin Sulfate Nanoparticles Inhibit Angiogenesis by Regulating the VEGFR2-Mediated PI3K/Akt Pathway.","authors":"Xia Zheng, Xiaofei Liu, Zhuo Wang, Rui Li, Qiaoli Zhao, Bingbing Song, Kit-Leong Cheong, Jianping Chen, Saiyi Zhong","doi":"10.3390/md23010022","DOIUrl":"10.3390/md23010022","url":null,"abstract":"Chondroitin sulfate (CS), a class of glycosaminoglycans covalently attached to proteins to form proteoglycans, is widely distributed in the extracellular matrix and cell surface of animal tissues. In our previous study, CS was used as a template for the synthesis of seleno-chondroitin sulfate (SeCS) through the redox reaction of ascorbic acid (Vc) and sodium selenite (Na2SeO3) and we found that SeCS could inhibit tumor cell proliferation and invasion. However, its effect on angiogenesis and its underlying mechanism are unknown. In this study, we analyzed the effect of SeCS on tube formation in vitro, based on the inhibition of tube formation and migration of human umbilical vein endothelial cells (HUVECs), and evaluated the in vivo angiogenic effect of SeCS using the chick embryo chorioallantoic membrane (CAM) assay. The results showed that SeCS significantly inhibited the angiogenesis of chicken embryo urothelium. Further mechanism analysis showed that SeCS had a strong inhibitory effect on VEGFR2 expression and its downstream PI3K/Akt signaling pathway, which contributed to its anti-angiogenic effects. In summary, SeCS showed good anti-angiogenic effects in an HUVEC cell model and a CAM model, suggesting that it may be a potential angiogenesis inhibitor.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11766607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salicylic Acid Improved the Growth of Dunaliella salina and Increased the Proportion of 9-cis-β-Carotene Isomers.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-01-01 DOI: 10.3390/md23010018

Shuaicheng Xiang, Xiaoting Qiu, Xiaojun Yan, Roger Ruan, Pengfei Cheng

{"title":"Salicylic Acid Improved the Growth of Dunaliella salina and Increased the Proportion of 9-cis-β-Carotene Isomers.","authors":"Shuaicheng Xiang, Xiaoting Qiu, Xiaojun Yan, Roger Ruan, Pengfei Cheng","doi":"10.3390/md23010018","DOIUrl":"10.3390/md23010018","url":null,"abstract":"Dunaliella salina is an important source of natural β-carotene (containing 9-cis and all trans isomers) for industrial production. The phytohormone salicylic acid (SA) has been proven to have impacts on the stress resistance of higher plants, but research on microalgae is currently unclear. In this study, the effects of SA on the growth, biochemical composition, antioxidant enzyme activity, key enzymes of β-carotene synthesis, and cis-and trans-isomers of β-carotene in D. salina under different salt concentrations were investigated. The results were shown that at concentrations of 1.5, 2, and 2.5 M NaCl, the antioxidant enzyme activity and key enzymes for β-carotene synthesis in algal cells were significantly increased, but the content and proportion of 9-cis isomer in β-carotene isomers decreased. The addition of SA significantly increased the growth and antioxidant enzyme (SOD, MDA) activity, as well as the synthesis of key enzyme phytoene synthase (PSY), phytoene desaturase (PDS), and lycopene β cyclase (LCYB) of D. salina under high-salinity conditions. It is worth noting that under the treatment of SA, the proportion of 9-cis isomer in the three salt concentrations (1.5, 2, 2.5 M NaCl) significantly increased by 32.09%, 20.30%, and 11.32%, respectively. Moreover, SA can not only improve the salt tolerance of D. salina, but also increase the proportion of 9-cis isomer, with higher physiological activity in β-carotene, thereby enhancing the application value of D. salina.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11766574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wild or Reared? Cassiopea andromeda Jellyfish as a Potential Biofactory.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-01-01 DOI: 10.3390/md23010019

Stefania De Domenico, Andrea Toso, Gianluca De Rinaldis, Marta Mammone, Lara M Fumarola, Stefano Piraino, Antonella Leone

{"title":"Wild or Reared? Cassiopea andromeda Jellyfish as a Potential Biofactory.","authors":"Stefania De Domenico, Andrea Toso, Gianluca De Rinaldis, Marta Mammone, Lara M Fumarola, Stefano Piraino, Antonella Leone","doi":"10.3390/md23010019","DOIUrl":"10.3390/md23010019","url":null,"abstract":"The zooxanthellate jellyfish Cassiopea andromeda (Forsskål, 1775), a Lessepsian species increasingly common in the western and central Mediterranean Sea, was investigated here to assess its potential as a source of bioactive compounds from medusa specimens both collected in the wild (the harbor of Palermo, NW Sicily) and reared under laboratory-controlled conditions. A standardized extraction protocol was used to analyze the biochemical composition of the two sampled populations in terms of protein, lipid, and pigment contents, as well as for their relative concentrations of dinoflagellate symbionts. The total extracts and their fractions were also biochemically characterized and analyzed for their in vitro antioxidant activity to quantify differences in functional compounds between wild and reared jellyfish. The two populations were similar in terms of extract yield, but with substantial differences in biomass, the number of zooxanthellae, protein and lipid contents, and fatty acid composition. The hydroalcoholic extracts obtained from jellyfish grown under controlled conditions showed greater antioxidant activity due to the presence of a higher content of bioactive compounds compared to wild jellyfish. This study could be the basis for considering the sustainable breeding of this holobiont or other similar organisms as a source of valuable compounds that can be used in the food, nutraceutical, or pharmaceutical sectors.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11767235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep-Sea Ecosystems as an Unexpected Source of Antibiotic Resistance Genes.

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-12-31 DOI: 10.3390/md23010017

Wei Zhang, Yingdong Li, Yunmeng Chu, Hao Liu, Hongmei Jing, Qianfeng Xia

{"title":"Deep-Sea Ecosystems as an Unexpected Source of Antibiotic Resistance Genes.","authors":"Wei Zhang, Yingdong Li, Yunmeng Chu, Hao Liu, Hongmei Jing, Qianfeng Xia","doi":"10.3390/md23010017","DOIUrl":"10.3390/md23010017","url":null,"abstract":"The deep-sea ecosystem, a less-contaminated reservoir of antibiotic resistance genes (ARGs), has evolved antibiotic resistance for microbes to survive and utilize scarce resources. Research on the diversity and distribution of these genes in deep-sea environments is limited. Our metagenomics study employed short-read-based (SRB) and assembled-contig-based (ACB) methods to identify ARGs in deep-sea waters and sediments and assess their potential pathogenicity. SRB prediction was found to be more effective for studying the abundance and diversity of these genes, while combining both methods better illustrated the relationship of ARGs with the hosts. Deep-sea waters (DSW) and trenches had the highest diversity of ARGs, including β-lactams, multidrug resistance genes, and rifamycins. Mobile genetic elements, such as IncQ and RP4 plasmids, were also identified. The ratio of nonsynonymous to synonymous substitutions (pN/pS) values of these genes suggest different evolutionary strategies in response to deep-sea conditions and possible human impacts. These resistome profiles provide valuable insights into their natural origins as well as the ecological and evolutionary implications of antibiotic resistance in deep-sea ecosystems. The exploration of the global distribution of ARGs in diverse deep-sea environments is a novel approach that will assist in understanding their potential reservoirs and evolutionary mechanisms. Therefore, employing a comprehensive approach to studying ARGs is particularly necessary. Unique microbial life in deep-sea ecosystems, especially in deep-sea cold seeps sediments (DSCSS), deep-sea waters (DSW), and trench waters (TW), could be a valuable source of new antibiotics and resistance discovery.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11766751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Sesterterpenoid, Petrosaspongin and γ-Lactone Sesterterpenoids with Leishmanicidal Activity from Okinawan Marine Invertebrates.

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-12-30 DOI: 10.3390/md23010016

Takahiro Jomori, Nanami Higa, Shogo Hokama, Trianda Ayuning Tyas, Natsuki Matsuura, Yudai Ueda, Ryo Kimura, Sei Arizono, Nicole Joy de Voogd, Yasuhiro Hayashi, Mina Yasumoto-Hirose, Junichi Tanaka, Kanami Mori-Yasumoto

{"title":"A Novel Sesterterpenoid, Petrosaspongin and γ-Lactone Sesterterpenoids with Leishmanicidal Activity from Okinawan Marine Invertebrates.","authors":"Takahiro Jomori, Nanami Higa, Shogo Hokama, Trianda Ayuning Tyas, Natsuki Matsuura, Yudai Ueda, Ryo Kimura, Sei Arizono, Nicole Joy de Voogd, Yasuhiro Hayashi, Mina Yasumoto-Hirose, Junichi Tanaka, Kanami Mori-Yasumoto","doi":"10.3390/md23010016","DOIUrl":"10.3390/md23010016","url":null,"abstract":"Leishmaniasis is a major public health problem, especially affecting vulnerable populations in tropical and subtropical regions. The disease is endemic in 90 countries, and with millions of people at risk, it is seen as one of the ten most neglected tropical diseases. Current treatments face challenges such as high toxicity, side effects, cost, and growing drug resistance. There is an urgent need for safer, affordable treatments, especially for cutaneous leishmaniasis (CL), the most common form. Marine invertebrates have long been resources for discovering bioactive compounds such as sesterterpenoids. Using bioassay-guided fractionations against cutaneous-type leishmaniasis promastigotes, we identified a novel furanosesterterpenoid, petrosaspongin from Okinawan marine sponges and a nudibranch, along with eight known sesterterpenoids, hippospongins and manoalides. The elucidated structure of petrosaspongin features a β-substituted furane ring, a tetronic acid, and a conjugated triene. The sesterterpenoids with a γ-butenolide group exhibited leishmanicidal activity against Leishmania major promastigotes, with IC50 values ranging from 0.69 to 53 μM. The structure-activity relationship and molecular docking simulation suggest that γ-lactone is a key functional group for leishmanicidal activity. These findings contribute to the ongoing search for more effective treatments against CL.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11766837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral Activity of the Marine Haptophyta Diacronema lutheri.

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-12-28 DOI: 10.3390/md23010012

Eleonora Montuori, Annalisa Ambrosino, Gerardo Della Sala, Costanza Ragozzino, Gianluigi Franci, Carla Zannella, Anna De Filippis, Donatella de Pascale, Massimiliano Galdiero, Chiara Lauritano

{"title":"Antiviral Activity of the Marine Haptophyta Diacronema lutheri.","authors":"Eleonora Montuori, Annalisa Ambrosino, Gerardo Della Sala, Costanza Ragozzino, Gianluigi Franci, Carla Zannella, Anna De Filippis, Donatella de Pascale, Massimiliano Galdiero, Chiara Lauritano","doi":"10.3390/md23010012","DOIUrl":"10.3390/md23010012","url":null,"abstract":"There are still several viral infections affecting a considerable number of the world's population, causing thousands of deaths each year. There are no drugs available for most viral infections and for many not even a vaccine. The marine kingdom is characterized by a huge chemical diversity; however, there is currently on the market only one drug derived from the sea with antiviral properties, called Ara-A. In the current study, we used a solid phase extraction method (SPE) to obtain pre-purified fractions from Diacronema lutheri raw extracts. We tested both raw extracts and fractions against enveloped and non-enveloped viruses. Results showed an antiviral activity of fraction C of D. lutheri against the herpes simplex virus type 1 (HSV-1 strain SC16). Liquid chromatography coupled with untargeted high-resolution tandem mass spectrometry (LC-HRMS2) were employed to chart the metabolite distribution in all SPE fractions and pinpoint molecular families unique (or almost unique) to the bioactive fraction. Sulfoquinovosyl di- and monoacylglycerols (SQDGs and SQMGs) and di- and monogalactosyl monoacylglycerols (DGMGs and MGMGs) represent the largest groups of compounds in fraction C and they are likely responsible for the antiviral properties of this fraction.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11766726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic Identification and Nutritional Analysis of Pterocladiella capillacea in Zhanjiang.

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-12-28 DOI: 10.3390/md23010011

Zhengwen Lv, Hongyan Cai, Nenghui Li, Hang Li, Jun Zeng, Kefeng Wu, Luming Deng, Huaqiang Tan, Hua Ye

{"title":"Taxonomic Identification and Nutritional Analysis of Pterocladiella capillacea in Zhanjiang.","authors":"Zhengwen Lv, Hongyan Cai, Nenghui Li, Hang Li, Jun Zeng, Kefeng Wu, Luming Deng, Huaqiang Tan, Hua Ye","doi":"10.3390/md23010011","DOIUrl":"10.3390/md23010011","url":null,"abstract":"To evaluate the nutritional value and development potential of Pterocladiella capillacea in the marine environment of Naozhou Island, Zhanjiang, this study conducted species classification and identification, followed by an analysis of key nutritional components. The combination of morphological and molecular results confirmed the identification of the collected samples as P. capillacea. Further analysis showed that P. capillacea in Zhanjiang had a moisture content of 74.9% and a protein content of 24%. In comparison, the fat (0.4%) and carbohydrate (15.4%) contents were relatively low, with moderate ash (14.3%) and crude fiber (9.1%) content. It contains a diverse range of fatty acids, with saturated fatty acids accounting for 51.82% and unsaturated fatty acids accounting for 48.18% of the total. The amino acid composition was also diverse, with essential amino acids comprising 31.58% and flavor-enhancing amino acids constituting 54.85%. The minerals contained four major elements and four trace elements, while heavy metal levels were within safety limits, ensuring their edibility. In conclusion, P. capillacea is a high-protein, low-fat economic seaweed with a favorable amino acid and fatty acid composition, rich in minerals, and with significant nutritional and developmental potential. This study provides important data to support future research and utilization of this seaweed.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11766499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Changes Under Heat Stress and Identification of Dendrillolactone, a New Diterpene Derivative with a Rare Rearranged Spongiane Skeleton from the Antarctic Marine Sponge Dendrilla antarctica.

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-12-28 DOI: 10.3390/md23010010

Andrea Prófumo, Conxita Avila, Adele Cutignano

{"title":"Chemical Changes Under Heat Stress and Identification of Dendrillolactone, a New Diterpene Derivative with a Rare Rearranged Spongiane Skeleton from the Antarctic Marine Sponge Dendrilla antarctica.","authors":"Andrea Prófumo, Conxita Avila, Adele Cutignano","doi":"10.3390/md23010010","DOIUrl":"10.3390/md23010010","url":null,"abstract":"The waters around the western Antarctic Peninsula are experiencing fast warming due to global change, being among the most affected regions on the planet. This polar area is home to a large and rich community of benthic marine invertebrates, such as sponges, tunicates, corals, and many other animals. Among the sponges, the bright yellow Dendrilla antarctica is commonly known for using secondary diterpenoids as a defensive mechanism against local potential predators. From the dichloromethane extract of sponge samples from Deception Island collected in January 2023, we isolated a novel derivative with an unusual β-lactone diterpene skeleton here named dendrillolactone (1), along with seven previously described diterpenes, including deceptionin (2), a gracilane norditerpene (3), cadlinolide C (4), a glaciolane norditerpene (5), membranolide (6), aplysulphurin (7), and tetrahydroaplysulphurine-1 (8). Here, we also report our studies on the changes in the chemical arsenal of this sponge by slow temperature increase in aquaria experiments. Despite being a species capable of inhabiting volcanically active areas, with frequent water temperature fluctuations due to the existing fumaroles, the results show that diterpenes such as deceptionin, cadlinolide C, membranolide, and tetrahydroaplysulphurin-1 seem to be susceptible to the temperature increase, resulting in a trend to higher concentrations. However, temperatures above 4 °C severely affected sponge metabolism, causing its death much earlier than expected. Further research on the roles of these natural products in D. antarctica and their relationship to the sponge's resilience to environmental changes should help to better understand the defensive mechanisms of Antarctic marine benthos in the context of global change.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11767012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-Friendly Extraction of Phlorotannins from Padina pavonica: Identification Related to Purification Methods Towards Innovative Cosmetic Applications.

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-12-28 DOI: 10.3390/md23010015

Moustapha Nour, Valérie Stiger-Pouvreau, Alain Guenneguez, Laurence Meslet-Cladière, Stéphane Cérantola, Ahmed Ali, Gaelle Simon, Abdourahman Daher, Sylvain Petek

{"title":"Eco-Friendly Extraction of Phlorotannins from Padina pavonica: Identification Related to Purification Methods Towards Innovative Cosmetic Applications.","authors":"Moustapha Nour, Valérie Stiger-Pouvreau, Alain Guenneguez, Laurence Meslet-Cladière, Stéphane Cérantola, Ahmed Ali, Gaelle Simon, Abdourahman Daher, Sylvain Petek","doi":"10.3390/md23010015","DOIUrl":"10.3390/md23010015","url":null,"abstract":"This study focuses on developing innovative and eco-friendly purification methods for the isolation of bioactive compounds derived from Padina pavonica, a brown abundant macroalga in Djibouti. Three distinct fractions, obtained via liquid-liquid extraction (LLE_FAE), solid-phase extraction (SPE_WE50), and flash chromatography (FC_EtOH20), were selected based on their high phenolic content and antioxidant activities. All fractions were also evaluated for their anti-ageing potential by assessing their ability to inhibit two vital skin-ageing enzymes, tyrosinase and elastase. Structural analysis by 1H-13C HMBC NMR and LC-MS revealed a selectivity of phlorotannins depending on the purification methods. The LLE_FAE fraction exhibited greater structural complexity, including compounds such as phloroglucinol, diphlorethol/difucol, fucophlorethol and bifuhalol, which likely contribute to its enhanced bioactivity compared to the fractions obtained by FC_EtOH20 and SPE_WE50, which were also active and enriched only in phloroglucinol and fucophlorethol. These findings highlight the impact of purification techniques on the selective enrichment of specific bioactive compounds and demonstrated the interest of FC or SPE in producing active phlorotannin-enriched fractions. These two purification methods hold strong potential for innovative cosmeceutical applications. Results are discussed regarding the use of P. pavonica as a promising marine resource in Djibouti to be used for the development of cosmetic industry.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11766817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic Hydrolysis Systems Enhance the Efficiency and Biological Properties of Hydrolysates from Frozen Fish Processing Co-Products.

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-12-28 DOI: 10.3390/md23010014

Maria Sapatinha, Carolina Camacho, Antónia Juliana Pais-Costa, Ana Luísa Fernando, António Marques, Carla Pires

{"title":"Enzymatic Hydrolysis Systems Enhance the Efficiency and Biological Properties of Hydrolysates from Frozen Fish Processing Co-Products.","authors":"Maria Sapatinha, Carolina Camacho, Antónia Juliana Pais-Costa, Ana Luísa Fernando, António Marques, Carla Pires","doi":"10.3390/md23010014","DOIUrl":"10.3390/md23010014","url":null,"abstract":"Co-products from the frozen fish processing industry often lead to financial losses. Therefore, it is essential to transform these co-products into profitable goods. This study explores the production of fish protein hydrolysates (FPH) from three co-products: the heads and bones of black scabbardfish (Aphanopus carbo), the carcasses of gilthead seabream (Sparus aurata), and the trimmings of Nile perch (Lates niloticus). Four enzymatic hydrolysis systems were tested: an endopeptidase (Alcalase, A), an exopeptidase (Protana, P), two-stage hydrolysis with an endopeptidase followed by an exopeptidase (A + P), and a single stage with endo- and exopeptidase (AP). The results show that combined enzymatic treatments, especially single-stage Alcalase and Protana (AP), achieved high protein yields (80%) and enhanced degrees of hydrolysis (34 to 49%), producing peptides with lower molecular weights. FPH exhibited significant antioxidant activity, in 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, with EC50 values below 5 mg/mL. Additionally, AP hydrolysates demonstrated over 60% angiotensin-converting enzyme (ACE) inhibition at 5 mg/mL, indicating potential antihypertensive applications. Antidiabetic and anti-Alzheimer activities were present, but at relatively low levels. AP hydrolysates, especially from gilthead seabream, proved to be the most promising. This study highlights the value of fish co-products as sources of functional peptides, contributing to waste reduction, and their potential applications in food, agriculture, and nutraceuticals.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11766955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0