Marine Drugs最新文献_第4页

New Strategy for the Degradation of High-Concentration Sodium Alginate with Recombinant Enzyme 102C300C-Vgb and the Beneficial Effects of Its Degradation Products on the Gut Health of Stichopus japonicus. 重组酶102C300C-Vgb降解高浓度海藻酸钠的新策略及其降解产物对日本刺参肠道健康的有益影响

IF 5.4 2区医学

Marine Drugs Pub Date : 2025-08-25 DOI: 10.3390/md23090339

Ziqiang Gu, Feiyu Niu, Peng Yang, Wenling Gong, Hina Mukhtar, Siyu Li, Yanwen Zheng, Yiling Zhong, Hanyi Cui, Jichao Li, Haijin Mou, Dongyu Li

{"title":"New Strategy for the Degradation of High-Concentration Sodium Alginate with Recombinant Enzyme 102C300C-Vgb and the Beneficial Effects of Its Degradation Products on the Gut Health of Stichopus japonicus.","authors":"Ziqiang Gu, Feiyu Niu, Peng Yang, Wenling Gong, Hina Mukhtar, Siyu Li, Yanwen Zheng, Yiling Zhong, Hanyi Cui, Jichao Li, Haijin Mou, Dongyu Li","doi":"10.3390/md23090339","DOIUrl":"10.3390/md23090339","url":null,"abstract":"High viscosity of alginate means a relatively low substrate concentration, which limits the efficiency of hydrolysis, resulting in one of the main challenges for the large-scale production of alginate oligosaccharides (AOS). In this study, a pilot-scale degradation product (PSDP) of the recombinant enzyme 102C300C-Vgb was produced for the first time at a substrate concentration of up to 20% sodium alginate. The optimal conditions for SA digestion were: enzyme dosage of 25 U/g, enzymatic temperature of 45 °C, enzymatic pH of 7.0, and enzymatic time of 24 h. Under these conditions, the yield of enzymatic hydrolysis was consistently in the range of 69% to 70%. The average molecular weight (Mw) of PSDP was 1496.36 Da, mainly containing oligosaccharides with degrees of polymerization ranging from 2 to 4. The low-Mw PSDP was subsequently applied in the diet of sea cucumber Stichopus japonicus. The results showed that the body wall weight of S. japonicus increased significantly after 40 days of feeding with a 0.09% PSDP-supplemented diet. Furthermore, PSDP-supplemented diet significantly increased the thickness of the serosal and submucosal layers and the width folds of mucosa of the sea cucumber gut. The abundance of pathogenic bacteria was reduced effectively, and that of beneficial bacteria increased significantly after being fed with PSDP. The results demonstrated that PSDP can serve as a digestive health enhancer for sea cucumbers, promoting their healthy growth.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marine Algal Metabolites as Cellular Antioxidants: A Study of Caulerpin and Caulerpinic Acid in Saccharomyces cerevisiae. 海洋藻类代谢物作为细胞抗氧化剂：酿酒酵母中茎木素和茎木素酸的研究。

IF 5.4 2区医学

Marine Drugs Pub Date : 2025-08-25 DOI: 10.3390/md23090338

Graziana Assalve, Paola Lunetti, Annalisa Fai, Antonio Terlizzi, Vincenzo Zara, Alessandra Ferramosca

{"title":"Marine Algal Metabolites as Cellular Antioxidants: A Study of Caulerpin and Caulerpinic Acid in Saccharomyces cerevisiae.","authors":"Graziana Assalve, Paola Lunetti, Annalisa Fai, Antonio Terlizzi, Vincenzo Zara, Alessandra Ferramosca","doi":"10.3390/md23090338","DOIUrl":"10.3390/md23090338","url":null,"abstract":"Oxidative stress caused by excessive reactive oxygen species (ROS) contributes to numerous chronic diseases. Marine green algae of the Caulerpa genus are rich in bioactive compounds with potential antioxidant activity.Objective: This study aimed to evaluate the intracellular antioxidant effects of caulerpin (CAU) and its derivative caulerpinic acid (CA) using Saccharomyces cerevisiae as a eukaryotic model.Methods: Yeast cells were pretreated with 1 μM of CAU or CA, or with 1 μM of resveratrol (RESV) as a positive control, then exposed to 2 mM of H2O2. Growth, ROS levels, oxidative damage markers, and antioxidant defenses were assessed.Results: Both CAU and CA significantly improved cell survival under oxidative stress, restoring growth rates (CAU: 0.129 h-1, CA: 0.137 h-1) and doubling times (CAU: 5.38 h, CA: 5.07 h) close to control values. Intracellular ROS accumulation, protein carbonylation, and lipid peroxidation were reduced to near-baseline levels. While catalase (Cat) and superoxide dismutase (Sod) activity remained unchanged, CAU and CA elevated intracellular glutathione (GSH) levels (1.6-1.8 fold) and preserved glutathione peroxidase (GPx) activity, compared to stressed cells without antioxidant pretreatment.Conclusions: CAU and CA act as effective intracellular antioxidants, primarily via ROS scavenging and GSH-dependent pathways. These findings support their potential as natural candidates for developing antioxidant-based therapies against ROS-related disorders.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postbiotics of Marine Origin and Their Therapeutic Application. 海洋生物制剂及其治疗应用。

IF 5.4 2区医学

Marine Drugs Pub Date : 2025-08-24 DOI: 10.3390/md23090335

Isabel M Cerezo, Olivia Pérez-Gómez, Sonia Rohra-Benítez, Marta Domínguez-Maqueda, Jorge García-Márquez, Salvador Arijo

{"title":"Postbiotics of Marine Origin and Their Therapeutic Application.","authors":"Isabel M Cerezo, Olivia Pérez-Gómez, Sonia Rohra-Benítez, Marta Domínguez-Maqueda, Jorge García-Márquez, Salvador Arijo","doi":"10.3390/md23090335","DOIUrl":"10.3390/md23090335","url":null,"abstract":"The increase in antibiotic-resistant pathogens has prompted the search for alternative therapies. One such alternative is the use of probiotic microorganisms. However, growing interest is now turning toward postbiotics-non-viable microbial cells and/or their components or metabolites-that can confer health benefits without the risks associated with administering live microbes. Marine ecosystems, characterized by extreme and diverse environmental conditions, are a promising yet underexplored source of microorganisms capable of producing unique postbiotic compounds. These include bioactive peptides, polysaccharides, lipoteichoic acids, and short-chain fatty acids produced by marine bacteria. Such compounds often exhibit enhanced stability and potent biological activity, offering therapeutic potential across a wide range of applications. This review explores the current knowledge on postbiotics of marine origin, highlighting their antimicrobial, anti-inflammatory, immunomodulatory, and anticancer properties. We also examine recent in vitro and in vivo studies that demonstrate their efficacy in human and animal health. Some marine bacteria that have been studied for use as postbiotics belong to the genera Bacillus, Halobacillus, Halomonas, Mameliella, Shewanella, Streptomyces, Pseudoalteromonas, Ruegeria, Vibrio, and Weissella. In conclusion, although the use of the marine environment as a source of postbiotics is currently limited compared to other environments, studies conducted to date demonstrate its potential.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Alginate Utilization Strategy in a Novel Marine Bacteroidetes: Insights from Roseihalotalea indica gen. nov. sp. nov. TK19036^T. 一种新型海洋拟杆菌门海藻酸盐利用策略的表征：来自Roseihalotalea indica的见解。

IF 5.4 2区医学

Marine Drugs Pub Date : 2025-08-24 DOI: 10.3390/md23090334

Zheng Fu, Shunqin You, Defang Wu, Runying Zeng, Kai Tang, Zhuhua Chan

{"title":"Characterization of Alginate Utilization Strategy in a Novel Marine Bacteroidetes: Insights from Roseihalotalea indica gen. nov. sp. nov. TK19036T.","authors":"Zheng Fu, Shunqin You, Defang Wu, Runying Zeng, Kai Tang, Zhuhua Chan","doi":"10.3390/md23090334","DOIUrl":"10.3390/md23090334","url":null,"abstract":"Alginate, a major polysaccharide in brown algae, is vital for the carbon cycling of the ocean ecosystem and holds promise for biotechnological applications. Marine Bacteroidetes, known for the ability to degrade complex polysaccharides, play an important role in the ocean carbon cycle; however, the detailed alginate degradation pattern remains to be further explored. In this study, an alginate utilization locus was identified in the genome of a new marine Bacteroidetes, Roseihalotalea indica gen. nov. sp. nov. TK19036T, and encodes two new alginate lyases, RiAlyPL6 and RiAlyPL17, which play potential roles in the degradation and utilization of alginate. RiAlyPL6 and RiAlyPL17 have distinct degradation products and substrate preferences, revealing the adaptation of the strain to utilize alginate with different M/G ratios. Based on the results in this paper, we have proposed a model for the degradation and utilization mechanism of alginate in Roseihalotalea indica gen. nov. sp. nov. TK19036T. All in all, our research provides a new insight into the alginate mechanisms within marine Roseihalotalea, and the two novel alginate lyases are excellent candidates for preparation and application.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Democratized Discovery of Microsclerodermin F as an Immunophilin Ligand. Microsclerodermin F作为一种亲免疫蛋白配体的民主化发现。

IF 5.4 2区医学

Marine Drugs Pub Date : 2025-08-24 DOI: 10.3390/md23090336

Manfred Auer, Malcolm D Walkinshaw, Jacqueline Dornan, Nhan T Pham, Xinru Xue, Miaomiao Liu, Ronald J Quinn, Eric M Ross, Abimael D Rodríguez, James J La Clair

引用次数: 0

Sea Anemone Stichodactyla Haddoni Venom: Extraction Method Dictates Composition and Functional Potency. 海葵毒：提取方法决定成分及功能效价。

IF 5.4 2区医学

Marine Drugs Pub Date : 2025-08-23 DOI: 10.3390/md23090333

Meiling Huang, Ming Li, Rong Zhu, Kailin Mao, Kun Pan, Xuefeidan Liu, Bingmiao Gao

{"title":"Sea Anemone Stichodactyla Haddoni Venom: Extraction Method Dictates Composition and Functional Potency.","authors":"Meiling Huang, Ming Li, Rong Zhu, Kailin Mao, Kun Pan, Xuefeidan Liu, Bingmiao Gao","doi":"10.3390/md23090333","DOIUrl":"10.3390/md23090333","url":null,"abstract":"Sea anemone venoms contain diverse toxins that have significant pharmacological potential, including anticancer, ecticidal, and immunotherapeutic properties. However, critically, the extraction methodology influences venom composition and bioactivity. This study characterized venom from Stichodactyla haddoni obtained via homogenization, electrical stimulation, and milking. Extraction yields varied significantly between methods: the homogenization, electrical stimulation, and milking of healthy sea anemones yielded crude venoms at rates of 17.8%, 3.4%, and 1.5%, respectively. SDS-PAGE revealed distinct protein banding patterns and concentrations, while RP-HPLC demonstrated method-dependent compositional differences. Comprehensive proteomic profiling identified 2370 proteins, encompassing both unique and shared components across extraction techniques. Label-free quantitative analysis confirmed significant variations in protein abundance that was attributable to the extraction method. Cytotoxicity assays against cancer cell lines revealed concentration-dependent inhibition, with milking-derived venom exhibiting the highest potency. Insecticidal activity against Tenebrio molitor was also method-dependent, with milking venom inducing the highest mortality rate. These findings elucidate the profound impact of extraction methodology on the protein composition and functional activities of S. haddoni venom, providing crucial insights for its optimized exploitation in pharmacological development.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Inter- and Intraspecific Variability in Amphidinol Content and Toxicity of Amphidinium Strains. 两种间和种内两种酚含量和毒性的高度变异性。

IF 5.4 2区医学

Marine Drugs Pub Date : 2025-08-22 DOI: 10.3390/md23090332

Catharina Alves-de-Souza, Jannik Weber, Mathew Schmitt, Robert York, Sarah Karafas, Carmelo Tomas, Bernd Krock

{"title":"High Inter- and Intraspecific Variability in Amphidinol Content and Toxicity of Amphidinium Strains.","authors":"Catharina Alves-de-Souza, Jannik Weber, Mathew Schmitt, Robert York, Sarah Karafas, Carmelo Tomas, Bernd Krock","doi":"10.3390/md23090332","DOIUrl":"10.3390/md23090332","url":null,"abstract":"Amphidinols (AM) are a diverse group of bioactive polyketides produced by dinoflagellates of the genus Amphidinium, known for their hemolytic, antifungal, and cytotoxic activities. This work presents the assessment of AM profiles in a comprehensive number of strains, whose species boundaries were previously established through detailed taxonomic analysis. Using UHPLC-MS/MS, we characterized the spectrum of AM analogs in 54 Amphidinium strains isolated from diverse geographical locations. In addition, toxicity was assessed using brine shrimp assays, which revealed significant inter- and intraspecific variability. Despite the broad diversity in AM content, no clear correlation was observed between total AM levels and toxicity across all strains. Multivariate analysis grouped the strains into clusters distinguished by distinct AM profiles and toxicity levels, suggesting that AM production alone does not predict toxicity. Our findings highlight the complexity of Amphidinium bioactivity, emphasizing the influence of strain-specific factors and other bioactive compounds. This work highlights the importance of integrating chemical, genetic, and biological assessments to understand better the factors that govern toxicity in this genus, with implications for ecological studies and the monitoring of harmful dinoflagellates.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and Assessment of Hypoglycemic Active Peptide from Natural Edible Pigment Phycobiliprotein Based on Molecular Docking, Network Pharmacology, Enzyme Inhibition Assay Analyses, and Cell Experiments. 基于分子对接、网络药理学、酶抑制分析和细胞实验的天然食用色素藻胆蛋白降糖活性肽筛选与评价

IF 5.4 2区医学

Marine Drugs Pub Date : 2025-08-17 DOI: 10.3390/md23080331

Zhimin Zhu, Yan Zhang, Bingbing He, Limin He, Guihong Fang, Yi Ning, Pengcheng Fu, Jing Liu

{"title":"Screening and Assessment of Hypoglycemic Active Peptide from Natural Edible Pigment Phycobiliprotein Based on Molecular Docking, Network Pharmacology, Enzyme Inhibition Assay Analyses, and Cell Experiments.","authors":"Zhimin Zhu, Yan Zhang, Bingbing He, Limin He, Guihong Fang, Yi Ning, Pengcheng Fu, Jing Liu","doi":"10.3390/md23080331","DOIUrl":"https://doi.org/10.3390/md23080331","url":null,"abstract":"Phycobiliproteins have gained increasing attention for their antidiabetic potential, yet the specific bioactive peptides and their targets and molecular mechanisms have remained unclear. In this study, four peptides with potential hypoglycemic activity were identified through virtual screening. Network pharmacology was employed to elucidate their hypoglycemic mechanism in the treatment of T2DM. A subsequent in vitro assay confirmed that the synthesized peptides, GR-5, SA-6, VF-6, and IR-7, exhibited significant inhibitory activity against α-glucosidase and DPP-IV. In insulin-resistant HepG2 models, all four peptides exhibited no cytotoxicity. Among them, GR-5 demonstrated the most promising therapeutic potential by remarkably enhancing cellular glucose consumption capacity. Furthermore, GR-5 administration substantially increased glycogen synthesis and enzymatic activities of hexokinase and pyruvate kinase with statistically significant improvements compared to the control groups. This study provides novel peptide candidates for T2DM treatment and validates an integrative strategy for targeted bioactive peptide discovery, advancing the development of algal protein-based therapeutics.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 8","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12387383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous Expression and Antimicrobial Targets of a Novel Glycine-Rich Antimicrobial Peptide from Artemia franciscana. 一种新型富甘氨酸抗菌肽的异源表达及抑菌靶点研究。

IF 5.4 2区医学

Marine Drugs Pub Date : 2025-08-17 DOI: 10.3390/md23080330

Ming Tao, Aobo Sun, Huishi Shao, Huaiyuan Ye, Guangming Yu, Daigeng Chen, Wei Zhang

{"title":"Heterologous Expression and Antimicrobial Targets of a Novel Glycine-Rich Antimicrobial Peptide from Artemia franciscana.","authors":"Ming Tao, Aobo Sun, Huishi Shao, Huaiyuan Ye, Guangming Yu, Daigeng Chen, Wei Zhang","doi":"10.3390/md23080330","DOIUrl":"https://doi.org/10.3390/md23080330","url":null,"abstract":"The growing problem of antimicrobial resistance in aquaculture, caused by the excessive and unregulated use of antibiotics, highlights the critical necessity for developing new anti-infective solutions. Based on the characteristics of glycine-rich antimicrobial peptides (AMPs) and transcriptomic data, an antimicrobial peptide, namely AfRgly1, was discovered in this study. Subsequently, the peptide was obtained through heterologous expression in E. coli, and its antibacterial spectrum was determined. Molecular dynamics simulation and molecular biology experiments were conducted to explore the antibacterial target of AfRgly1. Results showed that the mRNA expression level of AfRgly1 was significantly upregulated after Vibrio alginolyticus infection. AfRgly1 has broad-spectrum antibacterial activity targeting on bacterial cell membrane, and it may also interact with bacterial DNA. AfRgly1 displayed low selectivity for fish red blood cells. These results indicate that AfRgly1 is an antimicrobial peptide with considerable potential for application in the development of therapeutic agents.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 8","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12387859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of a Hepatoprotective Trinor-Sesterterpenoid from the Marine Fungus Talaromyces sp. Against Hepatic Ischemia-Reperfusion Injury. 海洋真菌Talaromyces sp.中抗肝缺血再灌注损伤的三酯萜类化合物的发现。

IF 5.4 2区医学

Marine Drugs Pub Date : 2025-08-16 DOI: 10.3390/md23080329

Wenxun Lan, Jian Cai, Liyan Yan, Xinyi Wu, Lisha Zhang, Chunmei Chen, Zhongqiu Liu, Xuefeng Zhou, Lan Tang

{"title":"Discovery of a Hepatoprotective Trinor-Sesterterpenoid from the Marine Fungus Talaromyces sp. Against Hepatic Ischemia-Reperfusion Injury.","authors":"Wenxun Lan, Jian Cai, Liyan Yan, Xinyi Wu, Lisha Zhang, Chunmei Chen, Zhongqiu Liu, Xuefeng Zhou, Lan Tang","doi":"10.3390/md23080329","DOIUrl":"https://doi.org/10.3390/md23080329","url":null,"abstract":"A new trinor-sesterterpenoid penitalarin D (1), with a 3,6-dioxabicyclo[3.1.0]hexane moiety, as well as two known compounds, penitalarin C (2) and nafuredin A (3), were obtained from the mangrove sediment-derived Talaromyces sp. SCSIO 41412. Their structures were determined by detailed NMR, MS spectroscopic analyses, and ECD calculations. Penitalarin D (1) and nafuredin A (3) showed toxicity or no toxicity against HepG2 cells at a concentration of 200 μM. The transcriptome sequencing and bioinformatics analysis revealed that 3 could be effective by regulating ferroptosis pathways in HepG2 cells, which was subsequently validated by RT-qPCR, demonstrating significant upregulation of ferroptosis-related genes. Pre-treatment with 3 could mitigate hypoxia-reoxygenation-induced damage in the oxygen glucose deprivation/reperfusion (OGD/R) cell model. Given the structural similarity of compounds 1, 2, and 3, we also screened compounds 1 and 2 in an AML12 OGD/R model. As no significant activity was observed, compound 3 was selected for subsequent in vivo studies. Subsequently, in vivo experiments demonstrated that 3 could significantly decrease pro-inflammatory cytokines and display the hepatoprotective effects against hepatic ischemia-reperfusion injury (HIRI). These findings identified nafuredin A (3) as a promising hepatoprotective agent for new drug development.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 8","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12387297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0