Marine Drugs最新文献

{"title":"Metabolic Responses of <i>Pyropia haitanensis</i> to Dehydration-Rehydration Cycles Revealed by Metabolomics.","authors":"Jian Wen, Jianzhi Shi, Muhan Meng, Kai Xu, Yan Xu, Dehua Ji, Wenlei Wang, Chaotian Xie","doi":"10.3390/md23050203","DOIUrl":"10.3390/md23050203","url":null,"abstract":"<p><p><i>Pyropia haitanensis</i> (T.J. Chang and B.F. Zheng) undergoes periodic dehydration and rehydration cycles, necessitating robust adaptive mechanisms. Despite extensive research on its physiological responses to desiccation stress, the comprehensive metabolic pathways and recovery mechanisms post-rehydration remain poorly understood. This study investigated the metabolic responses of <i>P. haitanensis</i> to varying degrees of desiccation stress using LC-MS and UPLC-MS/MS. Under mild dehydration, the thallus primarily accumulated sugars and proline, while moderate and severe dehydration triggered the accumulation of additional osmoprotectants like alanine betaine and trehalose to maintain turgor pressure and water retention. Concurrently, the alga activated a potent antioxidant system, including enzymes and non-enzymatic antioxidants, to counteract the increased reactive oxygen species levels and prevent oxidative damage. Hormonal regulation also plays a crucial role in stress adaptation, with salicylic acid and jasmonic acid upregulating under mild dehydration and cytokinins and gibberellin GA₁₅ accumulating under severe stress. Rehydration triggered the recovery process, with indole acetic acid, abscisic acid, and jasmonic acid promoting rapid cell recovery. Additionally, arachidonic acid, acting as a signaling molecule, induced general stress resistance, facilitating the adaptation of the thallus to the dynamic intertidal environment. These findings reveal <i>P. haitanensis</i>' metabolic adaptation strategies in intertidal environments, with implications for enhancing cultivation and stress resistance in this economically important seaweed.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Genome Sequence Analysis, Probiotic Potential, and Safety Assessment of the Marine Bacterium <i>Paraliobacillus zengyii</i> CGMCC1.16464.","authors":"Qianjin Fan, Mengqi Jiao, Haoyue Huangfu, Lan Chen, Beijie Li, Zhijie Cao, Xuelian Luo, Jianguo Xu","doi":"10.3390/md23050202","DOIUrl":"10.3390/md23050202","url":null,"abstract":"<p><p><i>Paraliobacillus zengyii</i> CGMCC1.16464 (<i>P. zengyii</i>) is a novel antiviral probiotic candidate strain. To ensure its safety as a potential probiotic, a safety evaluation was conducted in this study. The safety and functional potential of <i>P. zengyii</i> were systematically assessed through genomic bioinformatics analysis, in vitro experiments, and acute oral toxicity tests in mice. Genomic analysis revealed that <i>P. zengyii</i> is rich in genes related to carbohydrate and amino acid metabolisms and carries genes encoding antimicrobial and antiviral agents (such as ectoine, type III polyketide synthase, and lasso peptides). It also expresses gastrointestinal tolerance-related proteins (ClpC, GroEL, and ClpP). Its resistance to polymyxins is an inherent trait with no risk of plasmid-mediated transfer. In vitro experiments confirmed that <i>P. zengyii</i> is somewhat tolerant to bile salts and acidic environments and does not exhibit hemolytic or gelatinase activity. Importantly, an acute oral toxicity test in mice revealed that after intervention with high, medium, or low doses, no significant abnormalities in the body weight, organ index, or tissue morphology of the mice were observed. In conclusion, <i>P. zengyii</i> exhibited good safety and probiotic potential in terms of genomic safety, metabolic function, and in vitro and in vivo toxicities, providing a theoretical basis for the development of novel functional probiotics.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12112907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phycobilins Versatile Pigments with Wide-Ranging Applications: Exploring Their Uses, Biological Activities, Extraction Methods and Future Perspectives.","authors":"Celestino García-Gómez, Diana E Aguirre-Cavazos, Abelardo Chávez-Montes, Juan M Ballesteros-Torres, Alonso A Orozco-Flores, Raúl Reyna-Martínez, Ángel D Torres-Hernández, Georgia M González-Meza, Sandra L Castillo-Hernández, Marcela A Gloria-Garza, Miroslava Kačániová, Maciej Ireneusz-Kluz, Joel H Elizondo-Luevano","doi":"10.3390/md23050201","DOIUrl":"10.3390/md23050201","url":null,"abstract":"<p><p>Phycobiliproteins (PBPs), captivating water-soluble proteins found in cyanobacteria, red algae, and cryptophytes, continue to fascinate researchers and industries due to their unique properties and multifaceted applications. These proteins consist of chromophores called phycobilins (PBs), covalently linked to specific protein subunits. Major phycobiliproteins include phycocyanin (PC), allophycocyanin (APC), and phycoerythrin (PE), each distinguished by distinct absorption and emission spectra. Beyond their colorful properties, PBs exhibit a broad spectrum of biological activities, including antibacterial, antifungal, antiviral, and antidiabetic effects, making them valuable for pharmaceutical, biotechnological, and medical purposes. The extraction and purification methods for PBs have been optimized to enhance their bioavailability and stability, opening new avenues for industrial production. For this review, a comprehensive literature search was conducted using scientific databases such as PubMed, Scopus, and Web of Science, prioritizing peer-reviewed articles published between 2000 and 2025, with an emphasis on recent advances from the last five years, using keywords such as \"phycobiliproteins\", \"phycobilins\", \"bioactivities\", \"therapeutic applications\", and \"industrial use\". Studies were selected based on their relevance to the biological, technological, and pharmacological applications of PBPs and PBs. This review explores the diverse applications of PBs in therapeutic, diagnostic, and environmental fields, highlighting their potential as natural alternatives in the treatment of various diseases. The future perspectives for PBs focus on their incorporation into innovative drug delivery systems, biocompatible materials, and functional foods, presenting exciting opportunities for advancing human health and well-being.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jellyfish Collagen in the Mediterranean Spotlight: Transforming Challenges into Opportunities.","authors":"Ainara Ballesteros, Raquel Torres, Maria Pascual-Torner, Francisco Revert-Ros, Jose Tena-Medialdea, José Rafael García-March, Josep Lloret, Josep-Maria Gili","doi":"10.3390/md23050200","DOIUrl":"10.3390/md23050200","url":null,"abstract":"<p><p>Research increasingly highlights jellyfish as a sustainable alternative to other animal species, particularly for its collagen, which has versatile applications in blue biotechnology. This review explores the properties of jellyfish-derived collagen, extraction techniques, and its diverse industrial applications based on the current scientific literature. With a particular focus on research in the Mediterranean Sea, we underscore the role of the order Rhizostomeae as jellyfish species with high collagen content and provide an overview of the main sources for jellyfish harvesting, including active fishing, by-catch, and aquaculture. In the Mediterranean basin, the blooming species <i>Rhizostoma pulmo</i>, <i>Cotylorhiza tuberculata</i>, and <i>Rhopilema nomadica</i> represent a valuable opportunity to harness their nutraceutical benefits, as well as their potential for the development of biomaterials in tissue engineering and regenerative medicine. Although jellyfish fishing is not yet well-established in the region, ongoing collaborative projects with fishermen's guilds are focused on promoting circular and blue economy strategies to valorize jellyfish as an innovative resource. Additionally, jellyfish aquaculture emerges as a promising alternative for ensuring a sustainable supply, with the Rhizostomeae <i>Cassiopea</i> spp. demonstrating significant potential for biotechnological applications.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of Catalytic Activity and Thermostability of Alginate Lyase VxAly7B-CM via Rational Computational Design Strategies.","authors":"Xin Ma, Ke Zhu, Kaiyang Wang, Wenhui Liao, Xiaohan Yang, Wengong Yu, Weishan Wang, Feng Han","doi":"10.3390/md23050198","DOIUrl":"10.3390/md23050198","url":null,"abstract":"<p><p>Alginate lyase degrades alginate through the β-elimination mechanism to produce alginate oligosaccharides (AOS) with notable biochemical properties and diverse biological activities. However, its poor thermostability limits large-scale industrial production. In this study, we employed a rational computational design strategy combining computer-aided evolutionary coupling analysis and ΔΔG_fold evaluation to enhance both the thermostability and catalytic activity of the alginate lyase VxAly7B-CM. Among ten single-point mutants, the E188N and S204G mutants exhibited increases in <i>T</i>_m from 47.0 °C to 48.9 °C and 50.2 °C, respectively, with specific activities of 3701.02 U/mg and 2812.01 U/mg at 45 °C. Notably, the combinatorial mutant E188N/S204G demonstrated a Δ<i>T</i>_m of 5 °C and an optimal reaction temperature up to 50 °C, where its specific activity reached 3823.80 U/mg-a 31% increase. Moreover, its half-life at 50 °C was 38.4 h, which is 7.0 times that of the wild-type enzyme. Protein structural analysis and molecular dynamics simulations suggested that the enhanced catalytic performance and thermostability of the E188N/S204G mutant may be attributed to optimized surface charge distribution, strengthened hydrophobic interactions, and increased tertiary structure stability. Overall, our findings provided valuable insights into enzyme stabilization strategies and supported the industrial production of functional AOS.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12112969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental Resin Composites Modified with Chitosan: A Systematic Review.","authors":"Wojciech Dobrzyński, Paweł J Piszko, Jan Kiryk, Sylwia Kiryk, Mateusz Michalak, Agnieszka Kotela, Julia Kensy, Witold Świenc, Natalia Grychowska, Jacek Matys, Maciej Dobrzyński","doi":"10.3390/md23050199","DOIUrl":"10.3390/md23050199","url":null,"abstract":"<p><strong>Objective: </strong>This systematic review aims to evaluate the impact of incorporating chitosan into dental resin composites on their mechanical, antibacterial, and physicochemical properties.</p><p><strong>Methods: </strong>A comprehensive search of PubMed, Scopus, and Web of Science databases was conducted in March 2025 using the following keywords: resin, composite, and chitosan. The inclusion criteria comprised in vitro studies in dentistry evaluating chitosan as a composite additive with full-text availability in English. Data extraction and quality assessment were performed independently by multiple reviewers using standardized tools, and study quality was assessed based on predefined criteria.</p><p><strong>Results: </strong>Seventeen studies met the inclusion criteria. Chitosan concentrations in the composites ranged from 0.25 wt% to 20 wt%. Antibacterial activity was confirmed in six studies, especially against <i>S. mutans</i>, <i>S. sanguinis</i>, and <i>L. acidophilus</i>. Mechanical properties such as fracture toughness, hardness, and compressive strength were generally improved at lower concentrations of chitosan. However, increased chitosan levels were associated with decreased flexural strength and increased microleakage. Shear bond strength (SBS) was unaffected by chitosan in low concentrations (up to 0.25%), while higher concentrations reduced SBS. Fluoride release capacity was assessed in one study, with no significant differences observed.</p><p><strong>Conclusion: </strong>Chitosan-modified dental resin composites exhibit promising antibacterial and mechanical enhancements at low concentrations. However, higher chitosan levels may compromise certain mechanical and adhesive properties. These findings suggest a need for standardized methodologies and further research on long-term clinical implications and fluoride release.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Cytotoxic Activity of Secondary Metabolites from Marine-Derived <i>Penicillium</i> spp.: A Review (2018-2024).","authors":"Shuncun Zhang, Huannan Wang, Chunmei Sai, Yan Wang, Zhongbin Cheng, Zhen Zhang","doi":"10.3390/md23050197","DOIUrl":"10.3390/md23050197","url":null,"abstract":"<p><p>Marine-derived <i>Penicillium</i> spp., including <i>Penicillium citrinum</i>, <i>Penicillium chrysogenum</i>, and <i>Penicillium sclerotiorum</i>, have emerged as prolific producers of structurally diverse secondary metabolites with cytotoxic activity. This review systematically categorizes 177 bioactive compounds isolated from marine <i>Penicillium</i> spp. between 2018 and 2024, derived from diverse marine environments such as sediments, animals, plants, and mangroves. These compounds, classified into polyketides, alkaloids, terpenoids, and steroids, exhibit a wide range of cytotoxic activities. Their potency is categorized as potent (<1 μM or <0.5 μg/mL), notable (1-10 μM or 0.5-5 μg/mL), moderate (10-30 μM or 5-15 μg/mL), mild (30-50 μM or 15-25 μg/mL), and negligible (>50 μM or >25 μg/mL). The current review highlights the promising role of marine <i>Penicillium</i> spp. as a rich repository for the discovery of anticancer agents and the advancement of marine-inspired drug development.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite Profiling and Antioxidant Activities in Seagrass Biomass.","authors":"Pilar Garcia-Jimenez, Milagros Rico, Diana Del Rosario-Santana, Vicent Arbona, Marina Carrasco-Acosta, David Osca","doi":"10.3390/md23050193","DOIUrl":"10.3390/md23050193","url":null,"abstract":"<p><p>In this work, metabolite profiling of seeds and antioxidant analysis of fragments of two marine seagrasses, <i>Posidonia oceanica</i> and <i>Cymodocea nodosa,</i> were carried out to identify metabolite signature involved in seed viability and to evaluate the potential of fragments as a source of bioactive compounds. Using HILIC/QTOF-MS, UHPLC-MS and spectrophotometric analysis, seed metabolites and polyphenols and antioxidant activities, such as those of radical scavenging (RSA), reduction (FRAP, CUPRAC) and complexation (CCA), of rhizome fragments were evaluated. Metabolite comparison between seeds revealed differences across development stages (germinated and non-germinated) and seed types (dormant and non-dormant), providing insights into metabolic activity potentially associated with germination processes and seed viability. Furthermore, polyphenol analysis showed the highest content of caffeic acid in mature leaves (17.00 ± 0.02 μg g^-1 dw for <i>P. oceanica</i> and 98.00 ± 0.03 μg g^-1 dw for <i>C. nodosa</i>). Total phenolic content was correlated with flavonoids and with reduction and complexation activities. The combination of radical scavenging activity and t_1/2 was higher in <i>P. oceanica</i> than <i>C. nodosa</i> and also surpassed the commercial synthetic antioxidant BHA. We conclude <i>P. oceanica</i> and <i>C. nodosa</i> exhibit distinct seed metabolite profiles related to germination and type of seeds, and that fragments are rich in antioxidants, with potential as sustainable sources of bioactive compounds.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of L-Asparaginase-Producing Bacteria from the Arabian-Persian Gulf Region: First Report on <i>Bacillus xiamenensis</i> ASP-J1-4 as a Producer and Its Potential Application.","authors":"Ghofran M Al-Harbi, Essam Kotb, Abeer A Almiman, Mahmoud M Berekaa, Salwa Alhamad, Nada F Alahmady, Meneerah A Aljafary, Nadiyah M Alqazlan, Reem I Alyami, Joud M Alqarni, Ebtesam Abdullah Al-Suhaimi","doi":"10.3390/md23050194","DOIUrl":"10.3390/md23050194","url":null,"abstract":"<p><p>L-asparaginase (L-ASNase) functions as a chemotherapeutic enzyme with antitumor properties. It facilitates the degradation of L-asparagine (L-ASN), a vital amino acid required for the proliferation of tumor cells. In this study, we have isolated 177 L-ASNase-producing strains from the aquatic environment of the Arabian-Persian Gulf. The most potent isolate, ASP-J1-4, was an endophyte recovered from the seablite <i>Suaeda maritima</i> and was molecularly identified as <i>B. xiamenensis</i> (accession number PQ593941). The enzyme purified through DEAE-Sepharose displayed a molecular weight of 37 kDa based on the SDS-PAGE profile and lacked detectable L-glutaminase (L-GTNase) activity. Optimal enzyme activity was at 40 °C and pH 9.0, with stability at pH 7-9. The maximum stimulation effect was found in the presence of Fe³⁺, Mn²⁺, and Na⁺ ions, respectively. The enzyme demonstrated a <i>V</i>_max of 35.71 U/mL and a <i>K</i>_m of 0.15 mM. Interestingly, ASP-J1-4 L-ASNase showed a dose-dependent inhibition against human colon carcinoma (HCT-116) and cervical Henrietta Lacks (HeLa) cell lines, with IC₅₀ values of 15.42 µg/mL and 12.13 µg/mL, respectively. These findings collectively suggest a biocompatible, efficient, and robust enzyme for potential applications in tumor therapy after validation of in vivo studies and clinical trials. This study introduces the first deep screening program for L-ASNase-producing bacteria harboring in the Arabian-Persian Gulf region. In addition, it launches <i>B. xiamenensis</i> and other species as new sources of L-ASNase.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of TRPV1-Inhibitory Peptides from <i>Takifugu fasciatus</i> Skin Hydrolysate and Their Skin-Soothing Mechanisms.","authors":"Haiyan Tang, Bei Chen, Dong Zhang, Ruowen Wu, Kun Qiao, Kang Chen, Yongchang Su, Shuilin Cai, Min Xu, Shuji Liu, Zhiyu Liu","doi":"10.3390/md23050196","DOIUrl":"10.3390/md23050196","url":null,"abstract":"<p><p>Skin sensitivity is increasingly prevalent, necessitating new therapeutic agents. This study screened multifunctional peptides from <i>Takifugu fasciatus</i> skin for transient receptor potential vanilloid 1 (TRPV1)-inhibitory and anti-inflammatory activities and investigated their mechanisms in alleviating sensitive skin (SS). A low-molecular-weight hydrolysate was prepared through enzymatic hydrolysis of <i>T. fasciatus</i> skin, followed by ultrafiltration, with subsequent peptide identification performed using nano-HPLC-MS/MS and molecular docking-based virtual screening. Among 20 TRPV1-antagonistic peptides (TFTIPs), QFF (T10), LDIF (T14), and FFR (T18) exhibited potent anti-inflammatory effects in (lipopolysaccharide) LPS-induced RAW 264.7 macrophages. T14 showed the strongest TRPV1 inhibition; T14 (200 μM) inhibited Ca²⁺ in capsaicin-stimulated HaCaT cells by 73.1% and showed stable binding in molecular docking, warranting further analysis. Mechanistic studies revealed that T14 suppressed NF-κB signaling by downregulating p65 protein expression, thereby reducing pro-inflammatory cytokine secretion (G-CSF, GM-CSF, ICAM-1, IL-6, TNF-α) in RAW 264.7 cells. Additionally, T14 (400 μM) inhibited ET-1 in LPS-stimulated endothelial cells by 75.0%; ICAM-1 reached 49.0%. Network pharmacology predicted STAT3, MAPK3, SPHK1, and CTSB as key targets mediating T14's effects. These study findings suggest that T14 may be a promising candidate for skincare applications targeting SS.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 5","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}