Marine Drugs最新文献

{"title":"Genome-Based Mining of Carpatamides I-M and Their Candidate Biosynthetic Gene Cluster.","authors":"Shu-Mei Shen, Yun-Chang Xie, Li-Rong Tu, Miao-Er Wu, Yan-Min Wang, Chun-Hui Song, Yu-Hui Sun, Ming-He Luo","doi":"10.3390/md22110521","DOIUrl":"10.3390/md22110521","url":null,"abstract":"<p><p>Chemically investigating the marine-derived <i>Streptomyces parvus</i> 1268 led to the isolation of a new compound of carpatamide I (<b>1</b>). Subsequent genomic analysis identified its candidate biosynthetic gene cluster <i>ctd</i> of approximately 44 kb. In order to obtain more carpatamide derivatives, we conducted the upregulation of Ctd14, which is a positive regulator, and obtained improvement of carpatamide I and four new compounds of carpatamides J-M (<b>2</b>-<b>5</b>). The structures of the aforementioned five new isolates were identified by a combination of ESI-HRMS as well as one-dimensional (1D) and two-dimensional (2D) spectral NMR datasets. Bioassay results showed that compounds <b>1</b>-<b>5</b> displayed anti-inflammatory activity and weak cytotoxicity against cell lines of A549, HT-29, and HepG2.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Investigation of the Mediterranean Sponge <i>Crambe crambe</i> by UHPLC-HRMS/MS via Manual and Computational Dereplication Approaches.","authors":"Pinelopi Vlachou, Nikolaos Tsafantakis, Nikola Milic, Alexandros Polyzois, Eirini Baira, Aikaterini Termentzi, Géraldine Le Goff, Jamal Ouazzani, Nikolas Fokialakis","doi":"10.3390/md22110522","DOIUrl":"10.3390/md22110522","url":null,"abstract":"<p><p>The CH₂Cl₂-MeOH extract of the Mediterranean sponge <i>Crambe crambe</i> was investigated via UHPLC-HRMS/MS employing manual dereplication and in silico mass spectrometry tools. A deconvolution approach was implemented for the extensive metabolic characterization of the sample, resulting in the annotation of 53 compounds. The analysis of data-dependent HRMS/MS scans was conducted to establish fragmentation patterns characteristic of each crambescin A, B, and C sub-families. Among the 39 compounds identified from these groups, 22 analogues were reported for the first time including 4 new homologous series that differed by the ratio of methylene units in the upper (<i>n</i> + 2) and lower (<i>m</i> + 2) alkyl side chains. More specifically, crambescins presenting <i>m</i> = 5 or 6 and <i>n</i> = 5 (compounds <b>7</b>, <b>11</b>, <b>22</b> and <b>24</b>) as well as <i>m</i> = 5 or 6 and <i>n</i> = 4 (compounds <b>5</b>, <b>6</b>, <b>8</b>, <b>9</b>, 12 and 14) were characterized. Additionally, four new features, potentially corresponding to new crambescidin analogues (compounds <b>13</b>, <b>15</b>, <b>35</b>, and <b>39</b>), were also reported. The identity of the dereplicated features was further validated by studying crambescins' spectral similarities through a feature-based molecular networking approach. Overall, this study suggests UHPLC-HRMS/MS-through the integration of manual and computational dereplication approaches-as a valuable tool for the investigation and high-throughput characterization of the <i>C. crambe</i> metabolome.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Marine-Derived Components on Cardiovascular Disease Risk Factors and Gut Microbiota Diversity.","authors":"Ingrid Lamminpää, Amedeo Amedei, Cinzia Parolini","doi":"10.3390/md22110523","DOIUrl":"10.3390/md22110523","url":null,"abstract":"<p><p>Cardiovascular diseases (CVDs), which comprise coronary heart disease, hypertension, and stroke, collectively represent the number one cause of death globally. Atherosclerosis is the dominant cause of CVDs, and its risk factors are elevated levels of low-density lipoprotein cholesterol and triglycerides, hypertension, cigarette smoking, obesity, and diabetes mellitus. In addition, diverse evidence highlights the role played by inflammation and clonal haematopoiesis, eventually leading to immunity involvement. The human microbiota project and subsequent studies using next-generation sequencing technology have indicated that thousands of different microbial species are present in the human gut. Disturbances in the gut microbiota (GM) composition, i.e., gut dysbiosis, have been associated with diseases ranging from localised gastrointestinal disorders to metabolic and cardiovascular illnesses. Of note, experimental studies suggested that GM, host immune cells, and marine-derived ingredients work together to ensure intestinal wall integrity. This review discusses current evidence concerning the links among GM, marine-derived ingredients, and human inflammatory disease. In detail, we summarise the impact of fish-derived proteins/peptides and algae components on CVD risk factors and gut microbiome. Furthermore, we describe the interplay among these dietary components, probiotics/prebiotics, and CVDs.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine Fungi Bioactives with Anti-Inflammatory, Antithrombotic and Antioxidant Health-Promoting Properties Against Inflammation-Related Chronic Diseases.","authors":"Maria-Aliki Papikinou, Konstantinos Pavlidis, Paschalis Cholidis, Dimitrios Kranas, Theodora Adamantidi, Chryssa Anastasiadou, Alexandros Tsoupras","doi":"10.3390/md22110520","DOIUrl":"10.3390/md22110520","url":null,"abstract":"<p><p>Fungi play a fundamental role in the marine environment, being promising producers of bioactive molecules in the pharmacological and industrial fields, which have demonstrated potential health benefits against cardiovascular and other chronic diseases. This review pertains to the analysis of the lipid compositions across various species of marine fungi and their constantly discovered substances, as well as their anti-inflammatory, antioxidant, and antithrombotic effects. The health-promoting aspects of these microorganisms will be explored, through the investigation of several mechanisms of action and interference of their bioactives in biochemical pathways. Despite exceptional results in this field, the potential of marine microorganisms remains largely unexplored due to the limited number of specialists in marine microbiology and mycology, a relatively recent science with significant contributions and potential in biodiversity and biotechnology.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Marine Peptide from <i>Netunea arthritica cumingii</i> Against Gentamicin-Induced Hair Cell Damage in Zebrafish.","authors":"Hongbao Zheng, Ranran Zhu, Yun Zhang, Kechun Liu, Qing Xia, Peihai Li, Xiaoyue Sun, Chen Sun, Shanshan Zhang","doi":"10.3390/md22110519","DOIUrl":"10.3390/md22110519","url":null,"abstract":"<p><p>Auditory hair cell damage induced by aminoglycoside antibiotics (AmAn) leads to hearing loss, which has a serious effect on people's mental and physical health. This ototoxicity is thought to be related with the excessive accumulation of reactive oxygen species (ROS) in hair cells. However, therapeutic agents that protect hair cells are limited. Marine peptides have been shown to have excellent potential applications in disease prevention and treatment. Therefore, this study investigated the protective effects of an active peptide from <i>Neptunea arthritica cumingii</i> against AmAn-induced hair cell damage using the model of hair cell damage zebrafish. We identified the number, ultrastructure, and function of hair cells using fluorescence probes and scanning electron microscopy. The uptake of AmAn, ROS level, mitochondrial permeability transition pore, and apoptosis in hair cells were also tested by fluorescence labeling and TUNEL assay. The molecular mechanism for hair cell protection exerted by the peptide was detected by a real-time quantitative PCR assay. The results indicated that the peptide suppressed the uptake of AmAn but did not damage the function of hair cells mediating hearing. It also prevented ROS accumulation, decreased the occurrence of apoptosis, and rescued the abnormal opening and expressions of mitochondrial permeability transition pore and genes related to antioxidants. The peptide may be an effective therapeutic agent for AmAn-induced ototoxicity. In the future, we plan to use mammalian models to further investigate the otoprotective effect of the peptide.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Lipid Features and Valorization Potential of Atlantic Salmon (<i>Salmo salar</i>) Heads.","authors":"João Pedro Monteiro, Tiago Sousa, Tânia Melo, Carla Pires, António Marques, Maria Leonor Nunes, Ricardo Calado, M Rosário Domingues","doi":"10.3390/md22110518","DOIUrl":"10.3390/md22110518","url":null,"abstract":"<p><p>The sustainable utilization of co-products derived from the salmon processing industry is crucial for enhancing the viability and decreasing the environmental footprint of both capture and aquaculture operations. Salmon (<i>Salmo salar</i>) is one of the most consumed fish worldwide and a major species produced in aquaculture. As such, significant quantities of salmon co-products are produced in pre-commercialization processing/steaking procedures. The present study characterized a specific co-product derived from the processing of salmon: minced salmon heads. More specifically, this work aimed to reveal the nutritional profile of this co-product, with a special focus on its lipid content, including thoroughly profiling fatty acids and fully appraising the composition in complex lipids (polar lipids and triglycerides) for the first time. The antioxidant potential of lipid extracts from this salmon co-product was also studied in order to bioprospect lipid functional properties and possibly unveil new pathways for added-value applications. Our analysis indicated that these minced salmon heads are exceptionally rich in lipids. Oleic acid is the most prevalent fatty acid in this co-product, followed by palmitic acid, stearic acid, and linoleic acid. Moreover, relevant lipid indexes inferred from the fatty acid composition of this co-product revealed good nutritional traits. Lipidome analysis revealed that triglycerides were clearly the predominant lipid class present in this co-product while phospholipids, as well as ceramides, were also present, although in minimal quantities. The bioprospecting of antioxidant activity in the lipid extracts of the minced salmon heads revealed limited results. Given the high concentration of triglycerides, minced salmon heads can constitute a valuable resource for industrial applications from the production of fish oil to biodiesel (as triglycerides can be easily converted into fatty acid methyl esters), as well as possible ingredients for cosmetics, capitalizing on their alluring emollient properties. Overall, the valorization of minced salmon heads, major co-products derived from the processing of one of the most intensively farmed fish in the world, not only offers economic benefits but also contributes to the sustainability of the salmon processing industry by reducing waste and promoting a more efficient use of marine bioresources.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterocycles and a Sorbicillinoid from the Coral-Derived Fungus <i>Penicillium chrysogenum</i>.","authors":"Junjie Yang, Yuan Zong, Cili Wang, Kai Li, Yue Zhang, Pinglin Li","doi":"10.3390/md22110517","DOIUrl":"10.3390/md22110517","url":null,"abstract":"<p><p>A detailed chemical study of the culture of a coral-derived fungus <i>Penicillium chrysogenum</i> resulted in the isolation and identification of four new aromatic heterocycles chrysoquinazolinones A-B (<b>1</b>-<b>2</b>) and chrysobenzothiazoles A-B (<b>3</b>-<b>4</b>), along with a new sorbicillinoid 4-carboxylsorbicillin (<b>5</b>). Chrysoquinazolinones A-B (<b>1</b>-<b>2</b>) combine a quinazolinone fragment with a bicyclo[2.2.2]octane or a pyrrolidone moiety, respectively, demonstrating the unexpected structures of marine natural products. Chrysobenzothiazoles A-B (<b>3</b>-<b>4</b>) possess a benzothiazole system and are the second isolation of this class of skeleton compounds from marine organisms. The existence of the pair of enantiomers (±<b>3</b>) was deduced by chiral HPLC analysis. Their structures and absolute configurations were elucidated by detailed spectroscopic analysis, comparison with the literature data, single-crystal X-ray crystallographic analysis and TDDFT-ECD calculations. Compound <b>5</b> exhibited moderate cytotoxicity against K562 and NCI-H446 cell lines, with IC₅₀ values of 15.00 μM and 16.87 μM, respectively.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodiversity and Hemolytic Toxicity of the Genus <i>Heterocapsa</i> (Dinophyceae) in the Beibu Gulf, China.","authors":"Yixiao Xu, Nina Dzhembekova, Kirsty F Smith, Haifeng Gu, Uwe John, Huanda Xie, Yujuan Wen, Miao Wu","doi":"10.3390/md22110514","DOIUrl":"10.3390/md22110514","url":null,"abstract":"<p><p>The dinoflagellate genus <i>Heterocapsa</i> includes several widely distributed and potentially toxic species associated with Harmful Algal Blooms (HABs), particularly affecting the Western Pacific Ocean. To reveal the biodiversity of <i>Heterocapsa</i> in Beibu Gulf, six strains were morphologically characterized using light and scanning electron microscopy, while large subunit rDNA (LSU rDNA) and internal transcribed spacer (ITS) were sequenced for phylogenetic analysis through maximum likelihood and Bayesian inferences. Two strains (BGERL169, BGERL170) were identified as <i>Heterocapsa philippinensis</i> ribotype I, three (BGERL171-BGERL173) as a new <i>Heterocapsa philippinensis</i> ribotype II, and one strain (BGERL174) as <i>Heterocapsa pseudotriquetra</i>. Cells of <i>H. philippinensis</i> were ovoid to spherical, yellowish-brown, with reticulate chloroplasts, and had a sausage-shaped nucleus positioned longitudinally along the dorsal side of the cell, and the theca was arranged in Po, cp, X, 5', 3a, 7″, 6c, 5s, 5‴, 2⁗. Additionally, BGERL169 and BGERL171 showed no hemolytic toxicity in rabbit erythrocyte lysis assays. To the best of our knowledge, this research provides the first morphological and phylogenetic analysis of <i>H. philippinensis</i>, including the identification of a new ribotype, as well as the discovery of <i>H. pseudotriquetra</i> in Chinese waters. The findings contribute to the understanding of <i>Heterocapsa</i> species biogeography and toxicity in Chinese waters, offering valuable data for future HAB monitoring in Beibu Gulf.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Agaro-Oligosaccharides Produced by Marine Bacteria (<i>Rheinheimera</i> sp. (HY)) Possessing Agarose-Degrading Enzymes on Myotube Function.","authors":"Youshi Huang, Takuya Hirose, Jyh-Ming Tsai, Katsuya Hirasaka","doi":"10.3390/md22110515","DOIUrl":"10.3390/md22110515","url":null,"abstract":"<p><p>Agarase and its metabolites are reported to have applications in a variety of fields, but there have been few studies of the effects of agaro-oligosaccharide hydrolysate on muscle function. In this study, we analyzed the functionality of agarase and its metabolites in bacteria isolated from seawater. A bacterium with agar-degrading activity was isolated from Shimabara, Nagasaki, Japan. Through 16S rRNA sequence alignment, it was identified as being closely related to <i>Rheinheimera</i> sp. WMF-1 and was provisionally named <i>Rheinheimera</i> sp. (HY). Crude enzymes derived from this bacterium demonstrated an ability to hydrolyze various polysaccharides, including agar, agarose, and starch, with the highest specificity observed for agarose. The optimum pH and temperature were pH 10 and 50 °C. A glycoside bond specificity analysis of enzymatic activity indicated the cleavage of the α-linkage. Next, we investigated the functional effects of agaro-oligosaccharides on C2C12 myotubes. Treatment with 10-30 kDa oligosaccharides significantly increased the hypertrophy rate, diameter, and expression of myosin heavy-chain genes in C2C12 myotubes. These results indicate that the agaro-oligosaccharides produced by the enzymes identified in this study improve muscle mass, suggesting their potential contribution to muscle function.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-Assisted Extraction of Alginate from <i>Fucus vesiculosus</i> Seaweed By-Product Post-Fucoidan Extraction.","authors":"Viruja Ummat, Ming Zhao, Saravana Periaswamy Sivagnanam, Shanmugapriya Karuppusamy, Henry Lyons, Stephen Fitzpatrick, Shaba Noore, Dilip K Rai, Laura G Gómez-Mascaraque, Colm O'Donnell, Anet Režek Jambark, Brijesh Kumar Tiwari","doi":"10.3390/md22110516","DOIUrl":"10.3390/md22110516","url":null,"abstract":"<p><p>The solid phase byproduct obtained after conventional fucoidan extraction from the brown seaweed <i>Fucus vesiculosus</i> can be used as a source containing alginate. This study involves ultrasound-assisted extraction (UAE) of alginate from the byproduct using sodium bicarbonate. Response surface methodology (RSM) was applied to obtain the optimum conditions for alginate extraction. The ultrasound (US) treatments included 20 kHz of frequency, 20-91% of amplitude, and an extraction time of 6-34 min. The studied investigated the crude alginate yield (%), molecular weight, and alginate content (%) of the extracts. The optimum conditions for obtaining alginate with low molecular weight were found to be 69% US amplitude and sonication time of 30 min. The alginate extracts obtained were characterized using Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Ultrasound-assisted extraction involving a short treatment lasting 6-34 min was found to be effective in extracting alginate from the byproduct compared to the conventional extraction of alginate using stirring at 415 rpm and 60 °C for 24 h. The US treatments did not adversely impact the alginate obtained, and the extracted alginates were found to have similar characteristics to the alginate obtained from conventional extraction and commercial sodium alginate.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}