Marine Drugs最新文献

{"title":"Single-Cell Transcriptomic Analysis Reveals Cell Heterogeneity and Altered Signaling Pathways in Jellyfish Sting Patients.","authors":"Zhen Qin, Zhengfeng Hao, Chun Wang, Ning Lu, Peiju Qiu, Su Wang, Rilei Yu","doi":"10.3390/md23090358","DOIUrl":"10.3390/md23090358","url":null,"abstract":"<p><p>Jellyfish stings induce a range of symptoms, from localized irritation to life-threatening systemic reactions, yet the underlying immune mechanisms remain poorly understood. This study employed single-cell RNA sequencing (scRNA-seq) to analyze peripheral blood mononuclear cells (PBMCs) from a severely affected patient and healthy controls, uncovering the immune landscape at single-cell resolution and identifying the signaling pathways. We identified 11 major immune cell types, with a marked increase in CD14+ monocytes (81.86% of total cells) and significant reductions in T cells, B cells, and CD16+ monocytes in the envenomated patient. Subclustering revealed six monocyte and four neutrophil subsets, each displaying distinct functional profiles. Patient monocytes were enriched for MMP9+ and RETN+ subsets, associated with leukocyte migration and inflammation, whereas healthy controls exhibited CD74+ monocytes linked to oxidative phosphorylation. Neutrophils in the patient were predominantly LTF+ and S100A12+, implicating inflammatory and immune regulatory pathways. These findings provide a detailed single-cell atlas of immune dysregulation post-jellyfish sting, highlighting the pivotal roles of MMP9+ monocytes and S100A12+ neutrophils in driving inflammation. This study offers potential therapeutic targets for mitigating severe immune responses in jellyfish envenomation.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Synthesis of Marine-Inspired Itampolin A Derivatives to Overcome Chemoresistance in NSCLC via Cholesterol Homeostasis Modulation.","authors":"Hai-Ying Zhang, Shun-Chang Ji, Si-Hua Xie, Yu Chen, Cai-Xia Lin, Xu Huang, Yi-Qiao Wang, Jing-Wei Liang, Yan Liu","doi":"10.3390/md23090357","DOIUrl":"10.3390/md23090357","url":null,"abstract":"<p><p>Recent studies on brominated tyrosine-derived marine natural products have significantly expanded the library of known structures and revealed their potent and diverse antitumor mechanisms. Building upon our previous research on the natural product itampolin A isolated from marine sponges, we conducted structural optimizations and explored the structure--activity relationships (SARs) of novel scaffold derivatives concerning their inhibitory activities against lung cancer cells. In the present study, we further synthesized 15 novel derivatives, and compound <b>4l</b> demonstrated selective anti-proliferative activity against gefitinib-resistant PC9/GR cells, showing 4-fold greater potency compared to parental PC9 cells. Building on this finding, the present study aims to investigate the molecular mechanisms underlying the anti-proliferative effects of 4l in drug-resistant NSCLC models. Through cell cycle analysis, apoptosis assays, and signaling pathway evaluation, we seek to establish a theoretical foundation for developing novel therapeutic agents against chemotherapy-resistant lung cancer.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement Effect and Regulation Mechanism of Oyster Peptide on Dexamethasone-Induced Osteoporotic Rats.","authors":"Wei Yang, Wenyu Ma, Xiaoming Qin, Wenhong Cao, Haisheng Lin","doi":"10.3390/md23090356","DOIUrl":"10.3390/md23090356","url":null,"abstract":"<p><p>The increasing global population of the elderly and rising life expectancy have made osteoporosis a more severe public health issue, necessitating the development of safer and more effective therapeutic strategies. This study investigated the osteoprotective effects of low, medium, and high doses of oyster peptide (OP) in dexamethasone (DEX)-induced osteoporotic rats. Pathological analysis showed that OP treatment effectively mitigated bone loss and repaired bone microarchitecture deterioration caused by DEX administration. In the OP groups, levels of the osteogenic markers osteocalcin (OCN) and osteoprotegerin (OPG) were significantly higher than in the DEX group. Moreover, levels of the osteoclastic markers RANKL, Cathepsin K (Cath-K), MMP-9, C-terminal telopeptide of type I collagen (CTX-1), and Deoxypyridine (DPD) were significantly lower. Bone proteomic analysis of the DEX and OP groups revealed that differentially expressed proteins were significantly enriched in pathways related to extracellular matrix and structural reorganization, ECM-receptor interaction, and PI3K-Akt signaling. Furthermore, virtual screening simulations indicated that peptides with lengths ranging from 11 to 20 amino acid residues were involved in modulating the activity of key receptors in these pathways, including Integrins α5β1, Integrins αvβ3, and EGFR. Collectively, these results demonstrate the significant potential of OP as a novel therapeutic agent for osteoporosis.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phycobiliprotein Extract from <i>Arthrospira platensis</i> Boosts Immune Function in Pacific Oysters (<i>Magallana gigas</i>).","authors":"Aleksandra Andreyeva, Tatyana Kukhareva, Anastasiya Tkachuk, Maria Podolskaya, Elina Chelebieva, Andrey Borovkov","doi":"10.3390/md23090355","DOIUrl":"10.3390/md23090355","url":null,"abstract":"<p><p>The utilization of functional feeds in oyster hatcheries to reduce disease-related issues and improve health in the prespawning period is expected to become essential in the near future. In the present study, an aqueous extract of phycobiliproteins (CBPs) sourced from the cyanobacterium <i>Arthrospira platensis</i> was tested as an immunomodulatory agent in the Pacific oyster (<i>Magallana gigas</i>). Adult oysters were given three distinct treatments with the aqueous extract of CBPs (2, 20 or 80 μg/mL) for 24-96 h. In vivo analysis demonstrated that the extract of CBPs enhanced phagocytosis, lysosomal content and mitochondrial membrane potential levels, but inhibited the production of reactive oxygen species in hemocytes of oysters. Higher concentrations of the extract (80 μg/mL) had a more rapid effect on phagocytosis, with significant differences found after the first 24 h of the experiment. Lower concentrations of the extract (2 μg/mL) enhanced the phagocytic activity of hemocytes at later stages of its administration. Additionally, the expression profiles of the <i>hsp70</i> and <i>hsp90</i> genes were monitored in gills from oysters exposed to the extract at concentrations of 2, 20 and 200 μg/mL for 48 h, considering their roles in regulating the innate immune system in bivalves. The results show that <i>hsp70</i> expression was down-regulated during the first 24 h of administration, whereas it recovered to control levels after 48 h. In contrast, the expression levels of <i>hsp90</i> were up-regulated throughout the entire period of extract administration. Combined, the results of the present study show that the aqueous extract of CBPs from <i>A. platensis</i> can rapidly enhance the cellular immune response in Pacific oysters, and could potentially be used as an immunomodulator in bivalve hatcheries.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salinity Modulates Carbon Flux to Promote Squalene and PUFA Biosynthesis in the Marine Protist <i>Thraustochytrium</i>.","authors":"Yuetong Zhao, Xingyu Zhu, Nimra Riaz, Xiuping Liu, Jiaqian Li, Guangyi Wang","doi":"10.3390/md23090354","DOIUrl":"10.3390/md23090354","url":null,"abstract":"<p><p>Salinity is a key environmental factor regulating lipid metabolism in marine oleaginous protists. This study examined the impact of NaCl concentration on growth, glucose utilization, and lipid biosynthesis in <i>Thraustochytrium</i> sp. ATCC 26185. Moderate salinity (20 g/L) enhanced biomass and glucose uptake, while high salinity (45 g/L) induced osmotic stress yet significantly promoted squalene accumulation (17.27 mg/g), a 3.26-fold increase compared with 0 g/L NaCl (5.29 mg/g). Integrated transcriptomic and metabolomic analyses revealed that salinity-dependent activation of glycolysis, the TCA cycle, and the pentose phosphate pathway increased cellular ATP, NADH, and NADPH levels. Under salt stress, the mevalonate (MVA) pathway was transcriptionally upregulated, with key enzymes, including ACAT, HMGR, and IDI, showing marked induction, which supports enhanced carbon flux toward squalene biosynthesis. Despite SQS downregulation, squalene accumulation increased, likely due to elevated precursor availability and reduced flux to downstream sterol pathways. Concurrently, high salinity repressed expression of ACC, FAS-α, and FAS-β, reducing saturated fatty acid levels, while upregulation of PKSB-favored polyunsaturated fatty acid (PUFA) synthesis. These findings suggest that high-salt stress triggers transcriptional reprogramming, redirecting acetyl-CoA from fatty acid synthesis toward squalene and PUFA production. This study offers new insights into the metabolic plasticity of thraustochytrids and highlights salinity modulation as a promising strategy for enhancing high-value lipid yields in marine biotechnology.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of Primary and Secondary Metabolites of <i>Rugulopteryx okamurae</i> Seaweed: Assessing Bioactivity, Scalability, and Molecular Mechanisms.","authors":"Ana Minerva García-Cervantes, José A M Prates, José Luis Guil-Guerrero","doi":"10.3390/md23090351","DOIUrl":"10.3390/md23090351","url":null,"abstract":"<p><p><i>Rugulopteryx okamurae</i> is an invasive brown alga that has colonised Mediterranean and northeastern Atlantic coastlines, posing significant ecological and economic challenges. Its biomass is rich in structurally diverse metabolites-including polysaccharides (alginate, fucoidan, laminaran), phlorotannins, diterpenoids, fatty acids, and peptides-many of which exhibit notable antioxidant, anti-inflammatory, antimicrobial, and anticancer activities. Comparative assessment of extraction yields, structural features, and bioactivity data highlights phlorotannins and diterpenoids as particularly promising, demonstrating low-micromolar potencies and favourable predicted interactions with key inflammatory and apoptotic targets. Algal polysaccharides exhibit various bioactivities but hold strong potential for scalable and sustainable industrial applications. Emerging compound classes such as fatty acids and peptides display niche bioactivities; however, their structural diversity and mechanisms of action remain insufficiently explored. Insights from in vitro and in silico studies suggest that phlorotannins may modulate NF-<i>κ</i>B and MAPK signalling pathways, while diterpenoids are implicated in the induction of mitochondrial apoptosis. Despite these findings, inconsistent extraction methodologies and a lack of in vivo pharmacokinetic and efficacy data limit translational potential. To overcome these limitations, standardized extraction protocols, detailed structure-activity relationship (SAR) and pharmacokinetic studies, and robust in vivo models are urgently needed. Bioactivity-guided valorisation strategies, aligned with ecological management, could transform <i>R. okamurae</i> biomass into a sustainable source for functional foods, cosmetics, and pharmaceuticals applications.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical Approaches to the Rapid Characterisation of Marine Glycolipids in Bioproduct Discovery.","authors":"Sudarshan Dhakal, Tim D Nalder, Susan N Marshall, Colin J Barrow","doi":"10.3390/md23090352","DOIUrl":"10.3390/md23090352","url":null,"abstract":"<p><p>Glycolipids are structurally diverse amphiphilic molecules with potential as non-petrochemical-derived bioproducts, including surfactants, emulsifiers, and antioxidants. The different bioactivities associated with this range of glycolipid structures also present opportunities for dietary supplements, cosmetics, and pharmaceuticals. Marine glycolipids are underexplored due to challenges with purification and structural characterisation. Analytical approaches enabling efficient sample purification, isolation, and identification of target glycolipids are crucial to determining the bioactivity and functions of organisms such as shellfish and seaweed. This review summarises advances in analytical methods applicable to marine glycolipids, including extraction and enrichment methods tailored to specific subclasses. Thin-layer chromatography (TLC)-based rapid detection techniques developed for specific subclasses in complex biological samples are discussed, alongside structure identification methods based on liquid chromatography (LC)-electrospray ionisation (ESI)-tandem mass spectrometry (MS/MS). Hydrophilic interaction liquid chromatography (HILIC), reverse-phase liquid chromatography (RPLC), and supercritical fluid chromatography (SFC) coupled with MS detection are reviewed for their application to glycolipids. The application of two-dimensional liquid chromatography (2D-LC) and advanced MS-based approaches that facilitate both the rapid resolution and comprehensive characterisation of molecular species are also reviewed.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secondary Metabolites of the Marine Sponge-Derived Fungus <i>Aspergillus subramanianii</i> 1901NT-1.40.2 and Their Antimicrobial and Anticancer Activities.","authors":"Olga O Khmel, Anton N Yurchenko, Phan Thi Hoai Trinh, Ngo Thi Duy Ngoc, Vo Thi Dieu Trang, Huynh Hoang Nhu Khanh, Alexandr S Antonov, Konstantin A Drozdov, Roman S Popov, Natalya Y Kim, Dmitrii V Berdyshev, Ekaterina A Chingizova, Ekaterina S Menchinskaya, Ekaterina A Yurchenko","doi":"10.3390/md23090353","DOIUrl":"10.3390/md23090353","url":null,"abstract":"<p><p>The aim of this study was to investigate the metabolites in <i>Aspergillus subramanianii</i> 1901NT-1.40.2 extract using UPLC-MS, isolate and elucidate the structure of individual compounds, and study the antimicrobial and cytotoxic activities of the isolated compounds. The structures of two previously unreported ergostane triterpenoid aspersubrin A (<b>1</b>) and pyrazine alkaloid ochramide E (<b>2</b>) were established using NMR and HR ESI-MS. The absolute configuration of <b>1</b> was determined using quantum chemical calculations. Moreover, the known polyketides sclerolide (<b>3</b>) and sclerin (<b>4</b>); the indolediterpene alkaloid 10,23-dihydro-24,25-dehydroaflavinine (<b>5</b>); the bis-indolyl benzenoid alkaloids kumbicin D (<b>6</b>), asterriquinol D dimethyl ether (<b>7</b>), petromurin C (<b>8</b>); and the cyclopentenedione asterredione (<b>9</b>) were isolated. The effects of compounds <b>3</b>-<b>9</b> on the growth and biofilm formation of the yeast-like fungus <i>Candida albicans</i> and the bacteria <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> were investigated. Compounds <b>5</b> and <b>6</b> inhibited <i>C. albicans</i> growth and biofilm formation at an IC₅₀ of 7-10 µM. Moreover, the effects of compounds <b>3</b>-<b>9</b> on non-cancerous H9c2 cardiomyocytes, HaCaT keratinocytes, MCF-10A breast epithelial cells, and breast cancer MCF-7 and MDA-MB-231 cells were also investigated. Compound <b>8</b> (10 µM) significantly decreased the viability of MCF-7 cells, inhibited colony formation, and arrested cell cycle progression and proliferation in monolayer culture. Moreover, <b>8</b> significantly decreased the area of MCF-7 3D spheroids by approximately 30%. A competitive test with 4-hydroxytamoxyfen and molecular docking showed that estrogen receptors (ERβ more than ERα) were involved in the anticancer effect of petromurin C (<b>8</b>).</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Utilization of Polyguluronate by the Human Gut <i>Bacteroides</i> Species.","authors":"Nuo Liu, Ming Li, Xiangting Yuan, Tianyu Fu, Youjing Lv, Qingsen Shang","doi":"10.3390/md23090348","DOIUrl":"10.3390/md23090348","url":null,"abstract":"<p><p>Human gut <i>Bacteroides</i> species play crucial roles in the metabolism of dietary polysaccharides. Polyguluronate (PG), a major component of alginate, has been widely used in the food and medical industries. However, how PG is utilized by human gut <i>Bacteroides</i> species has not been fully elucidated. Here, using a combination of culturomics, genomics, and state-of-the-art analytical techniques, we elucidated in detail the utilization profiles of PG by 17 different human gut <i>Bacteroides</i> species. Our results indicated that each <i>Bacteroides</i> species exhibited a unique capability for PG utilization. Among all species tested, <i>Bacteroides xylanisolvens</i> consumed the highest amount of PG and produced the greatest quantity of short-chain fatty acids, suggesting that it may be a keystone bacterium in PG utilization. Mass spectrometry showed that PG was degraded by <i>B. xylanisolvens</i> into a series of oligosaccharides. Genomic analyses confirmed that <i>B. xylanisolvens</i> possesses a large and divergent repertoire of carbohydrate-active enzymes. Moreover, genomic annotation identified two enzymes, PL17_2 and PL6_1, in <i>B. xylanisolvens</i> that are potentially responsible for PG degradation. Altogether, our study provides novel insights into PG utilization by human gut <i>Bacteroides</i> species, which has important implications for the development of carbohydrate-based drugs from marine resources.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haemostatic and Biocompatibility Evaluation of Alginate-Functionalized Polylactide Composite Containing Zinc Sulphide and Hardystonite.","authors":"Anna Kaczmarek, Zdzisława Mrozińska, Jerzy J Chruściel, Michał Juszczak, Katarzyna Woźniak, Marcin H Kudzin","doi":"10.3390/md23090349","DOIUrl":"10.3390/md23090349","url":null,"abstract":"<p><p>The aim of this study was to evaluate the haemostatic potential and biocompatibility of a newly developed composite material for its use in blood-contacting applications. Based on promising reports on polylactide (PLA), sodium alginate (ALG), and bioactive additives such as hardystonite (HT) and zinc sulphide (ZnS), a melt-blown PLA nonwoven was modified via dip-coating using an ALG solution as a matrix for incorporating HT and ZnS particles, resulting in the PLA-ALG-ZnS-HT composite. The material was characterised in terms of surface morphology, specific surface area, pore volume, average pore size, and zeta potential (pH~7.4). Haemostatic activity was assessed by measuring blood coagulation parameters, while biocompatibility was evaluated through the viability of human peripheral blood mononuclear (PBM) cells and human foreskin fibroblasts (Hs68). Genotoxicity was analysed using the comet assay and plasmid relaxation test. Results confirmed a uniform alginate coating with dispersed HT and ZnS particles on PLA fibres. The modification increased the surface area and pore volume and caused a shift toward less negative zeta potential. Haemostatic testing showed prolonged activated partial thromboplastin time (aPTT), likely due to Zn²⁺ interactions with clotting factors. Biocompatibility tests showed high cell viability and no genotoxic effects. Our findings suggest that the PLA-ALG-ZnS-HT composite is safe for blood and skin cells and may serve as an anticoagulant material.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 9","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}