Marine Drugs最新文献_第2页

2-Acetamidophenol (2-AAP) Suppresses the Progression of Atherosclerosis by Alleviating Hyperlipidemia and Attenuating the Ferroptosis Pathway. 2-乙酰氨基苯酚（2-AAP）通过缓解高脂血症和减弱铁蛋白沉积途径抑制动脉粥样硬化的进展

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-11-13 DOI: 10.3390/md22110513

Xiaohan Zang, Yongcheng Wang, Cong Han, Lishuang Cui, Haojie Liu, Shuimiao Tian, Kechun Liu, Peihai Li, Chen Sun, Qing Xia, Yun Zhang

{"title":"2-Acetamidophenol (2-AAP) Suppresses the Progression of Atherosclerosis by Alleviating Hyperlipidemia and Attenuating the Ferroptosis Pathway.","authors":"Xiaohan Zang, Yongcheng Wang, Cong Han, Lishuang Cui, Haojie Liu, Shuimiao Tian, Kechun Liu, Peihai Li, Chen Sun, Qing Xia, Yun Zhang","doi":"10.3390/md22110513","DOIUrl":"10.3390/md22110513","url":null,"abstract":"Hyperlipidemia and consequent endothelial inflammation, along with foam cell generation, promote the progression of atherosclerosis (AS). Here, we aimed to investigate the effects of 2-acetamidophenol (2-AAP), which was selected by zebrafish phenotypic screening, in alleviating AS by relieving hyperlipidemia and inhibiting foam cell formation, as well as the underlying mechanisms. In a zebrafish hyperlipidemia model, 2-AAP increased lipid-lowering efficacy; alleviated TC, TG, LDL-C, and MDA levels; elevated HDL-C and T-SOD levels; significantly improved intravascular macrophage aggregation; and improved blood flow. In an ox-LDL-induced RAW264.7 model, 2-AAP inhibited lipid phagocytosis in RAW264.7 cells; reduced the intracellular TC, TG, FC, and CE contents; and decreased the CE/TC ratio, thus slowing foam cell generation. In addition, 2-AAP alleviated intracellular ROS and ferrous ion accumulation in RAW264.7 cells, reduced the MDA content, and increased GPX4 viability. Furthermore, transcriptome analyses and gene expression validation showed 2-AAP treatment upregulates genes related to GSH synthesis and transport, such as gclc, gclm, gss, and gpx4a, and enhanced the expression levels of genes involved in the storage and transportation of iron ions, such as fpn1, fth, and g6pd, indicating that 2-AAP dramatically regulated the ferroptosis and glutathione metabolic pathways. Overall, our study demonstrated that 2-AAP demonstrated potential in AS by alleviating hyperlipidemia and attenuating the ferroptosis pathway and provided evidence supporting the future application of 2-AAP in AS treatment.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hot-Melt Extrusion Drug Delivery System-Formulated Haematococcus pluvialis Extracts Regulate Inflammation and Oxidative Stress in Lipopolysaccharide-Stimulated Macrophages. 热熔挤压给药系统配制的血球菌提取物可调节脂多糖刺激的巨噬细胞的炎症和氧化应激。

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-11-13 DOI: 10.3390/md22110512

Tae-Young Gil, Ha-Yeon Sim, Ha-Yeon Lee, Suji Ryu, Jong-Suep Baek, Dae Geun Kim, Jaehoon Sim, Hyo-Jin An

{"title":"Hot-Melt Extrusion Drug Delivery System-Formulated Haematococcus pluvialis Extracts Regulate Inflammation and Oxidative Stress in Lipopolysaccharide-Stimulated Macrophages.","authors":"Tae-Young Gil, Ha-Yeon Sim, Ha-Yeon Lee, Suji Ryu, Jong-Suep Baek, Dae Geun Kim, Jaehoon Sim, Hyo-Jin An","doi":"10.3390/md22110512","DOIUrl":"10.3390/md22110512","url":null,"abstract":"Haematococcus pluvialis contains valuable bioactive compounds, including astaxanthin, proteins, and fatty acids. Astaxanthin is known for its various health benefits, such as preserving the redox balance and reducing inflammation. However, its low stability and poor water solubility present challenges for various applications. Hot-melt extrusion (HME) technology enhances the aqueous solubility of H. pluvialis extracts, increasing the usable astaxanthin content through nanoencapsulation (HME-DDS-applied extracts, ASX-60F and ASX-100F). This study compared the effects of HME-DDS-derived extracts (ASX-60F and ASX-100F) and the non-applied extract (ASX-C) under inflammatory and oxidative stress conditions. In animal models of sepsis, 60F and 100F treatment exhibited higher survival rates and a lower expression of pro-inflammatory biomarkers compared to those treated with C. In lipopolysaccharide-stimulated RAW 264.7 macrophages, nitric oxide (NO) production and the expression of pro-inflammatory mediators such as cyclooxygenase-2 and inducible NO synthase were reduced by 60F or 100F treatments via ERK/p-38 mitogen-activated protein kinase (MAPK) signaling. Moreover, 60F or 100F inhibited reactive oxygen species production regulated by nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling. Collectively, these findings suggest that HME-DDS-derived H. pluvialis extracts exert anti-inflammatory and antioxidant effects by inhibiting MAPK phosphorylation and activating Nrf2/HO-1 expression.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chaetomorpha linum Extract as a Source of Antimicrobial Compounds: A Circular Bioeconomy Approach. 作为抗菌化合物来源的亚麻茶提取物：循环生物经济方法。

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-11-13 DOI: 10.3390/md22110511

Roberta Barletta, Alfonso Trezza, Michela Geminiani, Luisa Frusciante, Tommaso Olmastroni, Filomena Sannio, Jean-Denis Docquier, Annalisa Santucci

{"title":"Chaetomorpha linum Extract as a Source of Antimicrobial Compounds: A Circular Bioeconomy Approach.","authors":"Roberta Barletta, Alfonso Trezza, Michela Geminiani, Luisa Frusciante, Tommaso Olmastroni, Filomena Sannio, Jean-Denis Docquier, Annalisa Santucci","doi":"10.3390/md22110511","DOIUrl":"10.3390/md22110511","url":null,"abstract":"The circular bioeconomy is currently a promising model for repurposing natural sources; these sources include plants due to their abundance of bioactive compounds. This study evaluated the antimicrobial properties of a Chaetomorpha linum extract. Chaetomorpha linum is an invasive macroalga from the Orbetello Lagoon (Tuscany, Italy), which grows in nutrient-rich environments and has been forming extended mats since 2005. The biomass is mechanically harvested and treated as waste, consuming considerable manpower and financial resources. As a potential way to increase the value of such waste, this study found that C. linum extract (CLE) is a source of antimicrobial compounds. The phytochemical characterization of the extract revealed the predominant presence of palmitic acid, a fatty acid with known antimicrobial activity. Based on such findings, four bacterial species of high clinical relevance (Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli) were tested, revealing a notable antibacterial activity of the extract on Enterococcus faecalis (MIC, 32 μg/mL). Computational analyses identified a potential Enterococcus faecalis molecular target for palmitic acid, offering molecular insights on the interaction. This study presents a comprehensive in vitro and in silico approach for drug and target discovery studies by repurposing C. linum as a source of antimicrobial bioactive compounds.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Microalgae Nannochloropsis oceanica and Chlorococcum amblystomatis Lipid Extracts Effects on UVA-Induced Changes in Human Skin Fibroblasts Proteome. 比较微藻 Nannochloropsis oceanica 和 Chlorococcum amblystomatis 脂质提取物对 UVA 诱导的人类皮肤成纤维细胞蛋白质组变化的影响

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-11-10 DOI: 10.3390/md22110509

Sinemyiz Atalay Ekiner, Agnieszka Gęgotek, Pedro Domingues, Maria Rosário Domingues, Elżbieta Skrzydlewska

{"title":"Comparison of Microalgae Nannochloropsis oceanica and Chlorococcum amblystomatis Lipid Extracts Effects on UVA-Induced Changes in Human Skin Fibroblasts Proteome.","authors":"Sinemyiz Atalay Ekiner, Agnieszka Gęgotek, Pedro Domingues, Maria Rosário Domingues, Elżbieta Skrzydlewska","doi":"10.3390/md22110509","DOIUrl":"10.3390/md22110509","url":null,"abstract":"Lipid extracts from the microalgae Nannochloropsis oceanica and Chlorococcum amblystomatis have great potential to prevent ultraviolet A (UVA)-induced metabolic disorders. Therefore, the aim of this study has been to analyze their cytoprotective effect, focused on maintaining intracellular redox balance and inflammation in UVA-irradiated skin fibroblasts, at the proteome level. The above lipid extracts reversed the suppression of the antioxidant response caused by UVA radiation, which was more visible in the case of C. amblystomatis. Modulations of interactions between heme oxygenase-1 and matrix metalloproteinase 1/Parkinson's disease protein 7/transcript1-α/β, as well as thioredoxin and migration inhibitory factor/Parkinson's disease protein 7/calnexin/ATPase p97, created key molecular signaling underlying their cytoprotective actions. Moreover, they reduced pro-inflammatory processes in the control group but they also showed the potential to regulate the cellular inflammatory response by changing inflammasome signaling associated with the changes in the caspase-1 interaction area, including heat shock proteins HSP90, HSPA8, and vimentin. Therefore, lipid extracts from N. oceanica and C. amblystomatis protect skin fibroblast metabolism from UVA-induced damage by restoring the redox balance and regulating inflammatory signaling pathways. Thus, those extracts have proven to have great potential to be used in cosmetic or cosmeceutical products to protect the skin against the effects of solar radiation. However, the possibility of their use requires the evaluation of their effects at the skin level in in vivo and clinical studies.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marine Algal Toxins and Public Health: Insights from Shellfish and Fish, the Main Biological Vectors. 海洋藻类毒素与公共健康：主要生物媒介贝类和鱼类的启示》。

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-11-10 DOI: 10.3390/md22110510

Kuan-Kuan Yuan, Hong-Ye Li, Wei-Dong Yang

{"title":"Marine Algal Toxins and Public Health: Insights from Shellfish and Fish, the Main Biological Vectors.","authors":"Kuan-Kuan Yuan, Hong-Ye Li, Wei-Dong Yang","doi":"10.3390/md22110510","DOIUrl":"10.3390/md22110510","url":null,"abstract":"Exposure to toxigenic harmful algal blooms (HABs) can result in widely recognized acute poisoning in humans. The five most commonly recognized HAB-related illnesses are diarrhetic shellfish poisoning (DSP), paralytic shellfish poisoning (PSP), amnesic shellfish poisoning (ASP), neurotoxic shellfish poisoning (NSP), and ciguatera poisoning (CP). Despite being caused by exposure to various toxins or toxin analogs, these clinical syndromes share numerous similarities. Humans are exposed to these toxins mainly through the consumption of fish and shellfish, which serve as the main biological vectors. However, the risk of human diseases linked to toxigenic HABs is on the rise, corresponding to a dramatic increase in the occurrence, frequency, and intensity of toxigenic HABs in coastal regions worldwide. Although a growing body of studies have focused on the toxicological assessment of HAB-related species and their toxins on aquatic organisms, the organization of this information is lacking. Consequently, a comprehensive review of the adverse effects of HAB-associated species and their toxins on those organisms could deepen our understanding of the mechanisms behind their toxic effects, which is crucial to minimizing the risks of toxigenic HABs to human and public health. To this end, this paper summarizes the effects of the five most common HAB toxins on fish, shellfish, and humans and discusses the possible mechanisms.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Polyhydroxysteroid Glycosides with Antioxidant Activity from the Far Eastern Sea Star Ceramaster patagonicus. 来自远东海星 Ceramaster patagonicus 的具有抗氧化活性的新型多羟基类固醇苷。

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-11-10 DOI: 10.3390/md22110508

Timofey V Malyarenko, Viktor M Zakharenko, Alla A Kicha, Arina I Ponomarenko, Igor V Manzhulo, Anatoly I Kalinovsky, Roman S Popov, Pavel S Dmitrenok, Natalia V Ivanchina

{"title":"New Polyhydroxysteroid Glycosides with Antioxidant Activity from the Far Eastern Sea Star Ceramaster patagonicus.","authors":"Timofey V Malyarenko, Viktor M Zakharenko, Alla A Kicha, Arina I Ponomarenko, Igor V Manzhulo, Anatoly I Kalinovsky, Roman S Popov, Pavel S Dmitrenok, Natalia V Ivanchina","doi":"10.3390/md22110508","DOIUrl":"10.3390/md22110508","url":null,"abstract":"Four new glycosides of polyhydroxysteroids, ceramasterosides A, B, D, and E (1-4), and two previously known compounds, ceramasteroside C1 (5) and attenuatoside B-I (6), were isolated from an extract of a deep-sea sea star species, the orange cookie star Ceramaster patagonicus. The structures of 1-4 were elucidated by the extensive NMR and ESIMS methods. Steroid monoglycosides 1 and 2 had a common 3β,6α,8,15β,16β-pentahydroxysteroid nucleus and a C-29 oxidized stigmastane side chain and differed from each other only in monosaccharide residues. Ceramasteroside A (1) contained 3-O-methyl-4-O-sulfated β-D-xylopyranose, while ceramasteroside B (2) had 3-O-methyl-4-O-sulfated β-D-glucopyranose, recorded from starfish-derived steroid glycosides for the first time. Their biological activity was studied using a model of lipopolysaccharide-induced (LPS) inflammation in a SIM-A9 murine microglial cell line. During the LPS-induced activation of microglial cells, 1, 3, and 5, at a non-toxic concentration of 1 µM, showed the highest efficiency in reducing the production of intracellular NO, while 4 proved to be most efficient in reducing the extracellular nitrite production. All the test compounds reduced the LPS-induced malondialdehyde (MDA) production. The in vitro experiments have demonstrated, for the first time, the antioxidant activity of the compounds under study.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salmon Nasal Cartilage-Derived Proteoglycans Alleviate Monosodium Iodoacetate-Induced Osteoarthritis in Rats. 鲑鱼鼻软骨衍生蛋白多糖可缓解碘乙酸钠诱发的大鼠骨关节炎

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-11-08 DOI: 10.3390/md22110507

Inae Jeong, Jinbum Park, Shinjung Park, Tatuya Wada, Dae Soo Lim, Ok-Kyung Kim

{"title":"Salmon Nasal Cartilage-Derived Proteoglycans Alleviate Monosodium Iodoacetate-Induced Osteoarthritis in Rats.","authors":"Inae Jeong, Jinbum Park, Shinjung Park, Tatuya Wada, Dae Soo Lim, Ok-Kyung Kim","doi":"10.3390/md22110507","DOIUrl":"10.3390/md22110507","url":null,"abstract":"Osteoarthritis is a chronic inflammatory condition characterized by the degeneration of joint cartilage and underlying bone, resulting in pain, swelling, and reduced mobility. This study evaluates the efficacy of salmon nasal cartilage-derived proteoglycans in mitigating osteoarthritis symptoms and investigates the underlying molecular mechanisms. This study employed a rat model of osteoarthritis induced by monosodium iodoacetate (MIA) injection. The rats were orally administered salmon nasal cartilage-derived proteoglycans or ibuprofen. Key aspects of osteoarthritis pathology, including impaired exercise ability, inflammation, extracellular matrix degradation, and chondrocyte apoptosis, were assessed using histological analysis, micro-CT, treadmill testing, serum assays, and mRNA/protein expression studies. The MIA injection caused significant cartilage damage, reduced bone mineral density, and impaired exercise ability. Additionally, it elevated serum levels of prostaglandin E2 and nitric oxide, increased the mRNA and protein levels of inflammation-related factors, and activated apoptosis signaling pathways in cartilage. Treatment with salmon nasal cartilage-derived proteoglycans significantly improved cartilage morphology and mineralization, reduced inflammation, and inhibited apoptosis signaling pathways, with effects comparable to those observed with ibuprofen treatment. These findings highlight the potential of salmon nasal cartilage-derived proteoglycans as a therapeutic agent for managing osteoarthritis by effectively reducing inflammation, preventing cartilage degradation, and inhibiting chondrocyte apoptosis.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aeruginosin 525 (AER525) from Cyanobacterium Aphanizomenon Sp. (KUCC C2): A New Serine Proteases Inhibitor. Aeruginosin 525 (AER525) from Cyanobacterium Aphanizomenon Sp. (KUCC C2)：一种新的丝氨酸蛋白酶抑制剂。

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-11-08 DOI: 10.3390/md22110506

Donata Overlingė, Marta Cegłowska, Robert Konkel, Hanna Mazur-Marzec

引用次数: 0

Influence of Silicate Concentrations on Growth, Carotenoid, and Fatty Acid Profiles of the Marine Diatom Conticribra weissflogii. 硅酸盐浓度对海洋硅藻 Conticribra weissflogii 的生长、类胡萝卜素和脂肪酸特征的影响。

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-11-06 DOI: 10.3390/md22110504

David Kwame Amenorfenyo, Feng Li, Xiangyu Rui, Xianghu Huang, Changling Li

{"title":"Influence of Silicate Concentrations on Growth, Carotenoid, and Fatty Acid Profiles of the Marine Diatom Conticribra weissflogii.","authors":"David Kwame Amenorfenyo, Feng Li, Xiangyu Rui, Xianghu Huang, Changling Li","doi":"10.3390/md22110504","DOIUrl":"10.3390/md22110504","url":null,"abstract":"Enhancing microalgal growth and bioactive compound production is becoming a duty for improving photosynthetic microorganisms. In this study, the growth, carotenoid, and fatty acid profiles of Conticribra weissflogii were studied under four different silicate concentrations and silicate-deficient conditions in an f/2 medium with continuous aeration, light intensity (30 ± 2 µmol m-2 s-1), salinity (25 ± 2‱), pH (8), and temperature (25 ± 2 °C). At the end of the experiment, we observed that a silicate concentration of 120 mg L-1 produced the maximum biomass dry weight (0.86 g L-1), carotenoid content (1.63 µg mL-1), and fucoxanthin content (1.23 mg g-1) in C. weissflogii. The eicosapentaenoic acid (EPA) (11,354.37 µg g-1), docosahexaenoic acid (DHA) (2516.16 µg g-1), gamma-linolenic acid (C18:3n6) (533.51 µg g-1), and arachidonic acid (C20:4n6) (1261.83 µg g-1) contents were significantly higher at Si 120 mg L-1. The results further showed the maximum fatty acid content in C. weissflogii at Si 120 mg L-1. However, the silicate-deficient conditions (Si 0 mg L-1) resulted in higher levels of saturated fatty acids (38,038.62 µg g-1). This study presents a practical approach for the large-scale optimization of biomass, carotenoid, fucoxanthin, and fatty acid production in C. weissflogii for commercial purposes.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moisturizing Effects of Alcalase Hydrolysate Fractions from Haliotis discus Viscera, a Marine Organism, on Human Dermal Fibroblasts, HaCaT Keratinocytes, and Reconstructed Human Skin Tissues. 海洋生物 Haliotis discus Viscera 的钙化酶水解物馏分对人体真皮成纤维细胞、HaCaT 角质细胞和重建人体皮肤组织的保湿作用

IF 4.9 2区医学

Marine Drugs Pub Date : 2024-11-06 DOI: 10.3390/md22110503

Nalae Kang, Eun-A Kim, Seong-Yeong Heo, Jun-Ho Heo, Ginnae Ahn, Soo-Jin Heo

{"title":"Moisturizing Effects of Alcalase Hydrolysate Fractions from Haliotis discus Viscera, a Marine Organism, on Human Dermal Fibroblasts, HaCaT Keratinocytes, and Reconstructed Human Skin Tissues.","authors":"Nalae Kang, Eun-A Kim, Seong-Yeong Heo, Jun-Ho Heo, Ginnae Ahn, Soo-Jin Heo","doi":"10.3390/md22110503","DOIUrl":"10.3390/md22110503","url":null,"abstract":"Haliotis discus, an abalone, is a marine gastropod mollusk that has been cultivated globally owing to its nutritional value and high market demand. However, the visceral parts of H. discus are typically discarded as by-products, highlighting the need to explore their potential value in developing cosmeceuticals and pharmaceuticals. This study investigated the potential moisturizing effects of H. discus visceral tissues. Various hydrolysates from H. discus viscera tissue were evaluated for proximate composition, radical scavenging, and hyaluronidase inhibition activities. Alcalase hydrolysate was isolated using gel filtration chromatography (GFC), and its moisturizing effects were tested on human dermal fibroblasts (HDF), HaCaT keratinocytes, and reconstructed human skin tissue. The Alcalase hydrolysate showed the highest extraction yield, radical scavenging, and hyaluronidase inhibition activities. The Alcalase hydrolysate GFC fraction 1 increased collagen synthesis-related molecules, including procollagen type 1 in HDF and hyaluronic acid-related molecules in HaCaT cells. These moisturizing effects were confirmed in reconstructed human skin tissues by increased levels of aquaporin 3 and filaggrin. Fraction 1 consisted of two main peptides: DNPLLPGPPF and SADNPLLPGPPF. In conclusion, H. discus Alcalase hydrolysate and its fractions have potential moisturizing properties and can be used as cosmeceuticals.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"22 11","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0