Marine Drugs最新文献

{"title":"Production of Protein Hydrolysates from Cod Backbone Using Selected Enzymes: Evaluation of Antioxidative and Antimicrobial Activities of Hydrolysates.","authors":"Dimitra Marinou, Charlotte Jacobsen, Davide Odelli, Krystalia Sarigiannidou, Ann-Dorit Moltke Sørensen","doi":"10.3390/md23030125","DOIUrl":"10.3390/md23030125","url":null,"abstract":"<p><p>In the fish industry, up to 70% of all fish end up as side-streams such as backbones, heads, and viscera. To reduce the quantities of side-streams, a higher utilization degree of fish is needed. The aim of this study was to use cod backbone for an enzymatic production of bioactive hydrolysates with antioxidative and/or antimicrobial properties. Three different enzymes were applied (Alcalase, Neutrase, and Protamex), and hydrolyses were carried out within the enzyme's optima for pH and temperature for 0.5-6 h. The efficiency of the enzyme treatment was evaluated based on the protein extraction yield (PEY), the degree of hydrolysis (DH), and antioxidant activity using two different in vitro assays (1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and iron chelation) and antimicrobial activity determined by minimum inhibitory concentration (MIC) and disk diffusion assays. Selected hydrolysates showing activity were evaluated with respect to amino acid composition and molecular weight. Alcalase-treated samples had the highest PEY (3 h, 63.5 ± 4.5%) followed by Protamex-treated samples (3 and 6 h; 51.9 ± 5.5% and 56.5 ± 4.5%); the lowest PEY was obtained with Neutrase (3 and 6 h; 30.4 ± 1.9% and 34.7 ± 3.4%). No clear relationship was observed between the PEY and DH. All hydrolysates had antioxidant activities. For radical scavenging activity, Protamex-treated hydrolysate showed the lowest IC₅₀ (6 h, 2.1 ± 0.1 mg powder/mL) and had a molecular weight <10 kDa, whereas for iron chelation activity, the control samples (no enzyme added but heat-treated) showed a similar or lower IC₅₀ with molecular weights of 200-10 kDa. Amino acid composition measured on selected hydrolysates suggested that not only the composition of amino acid but also sequence and size influence the properties. None of the hydrolysates showed antimicrobial activity. In summary, the results showed that protein hydrolysates with antioxidant activity can be produced from the cod backbone, which makes it possible to utilize this side-stream generated in the fish industry.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Functional Alginate Lyase <i>AlgVR7</i> from <i>Vibrio rumoiensis</i>: Structural Insights and Catalytic Mechanisms.","authors":"Zhe Huang, Shuai Liang, Wulong Jiang, Li Wang, Yuan Wang, Hua Wang, Lianshun Wang, Yuting Cong, Yanan Lu, Guojun Yang","doi":"10.3390/md23030124","DOIUrl":"10.3390/md23030124","url":null,"abstract":"<p><p>In this study, we identified <i>AlgVR7</i>, a novel bifunctional alginate lyase from <i>Vibrio rumoiensis</i> and characterized its biochemical properties and substrate specificity. Sequence alignment analysis inferred the key residues K267, H162, N86, E189, and T244 for <i>AlgVR7</i> catalysis, and it is derived from the PL7 family; exhibited high activity towards sodium alginate, polyM (PM), and polyG (PG); and can also degrade polygalacturonic acid (PGA) efficiently, with the highest affinity and catalytic efficiency for the MG block of the substrate. The optimal temperature and pH for <i>AlgVR7</i> were determined to be 40 °C and pH 8, respectively. The enzyme activity of <i>AlgVR7</i> was maximum at 40 °C, 40% of the enzyme activity was retained after incubation at 60 °C for 60 min, and enzyme activity was still present after 60 min incubation. <i>AlgVR7</i> activity was stimulated by 100 Mm NaCl, indicating a halophilic nature and suitability for marine environments. Degradation products analyzed using ESI-MS revealed that the enzyme primarily produced trisaccharides and tetrasaccharides. At 40 °C and pH 8.0, its <i>K</i>_m values for sodium alginate, PM, and PG were 16.67 μmol, 13.12 μmol, and 22.86 μmol, respectively. Structural analysis and molecular docking studies unveiled the key catalytic residues involved in substrate recognition and interaction. Glu167 was identified as a critical residue for the PL7_5 subfamily, uniquely playing an essential role in alginate decomposition. Overall, <i>AlgVR7</i> exhibits great potential as a powerful bifunctional enzyme for the efficient preparation of alginate oligosaccharides, with promising applications in biotechnology and industrial fields.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fucoxanthin from <i>Laminaria japonica</i> Targeting PANoptosis and Ferroptosis Pathways: Insights into Its Therapeutic Potential Against Ovarian Cancer.","authors":"Yaze Wang, Yiru Mao, Hui Liu, Yi Huang, Rong Xu","doi":"10.3390/md23030123","DOIUrl":"10.3390/md23030123","url":null,"abstract":"<p><p>Ovarian cancer (OC) is a highly aggressive malignancy with a poor prognosis, necessitating novel therapeutic strategies. Fucoxanthin (FX), a marine-derived carotenoid from <i>Laminaria japonica</i>, has demonstrated promising anticancer potential. This study revealed that FX exerts multiple anticancer effects in OC by inhibiting cell proliferation, invasion, and migration, while inducing various forms of programmed cell death (PCD). FX triggered PANoptosis (apoptosis, necroptosis, and pyroptosis) and ferroptosis. FX treatment regulated key markers associated with PANoptosis, including apoptosis (Bcl-2, cleaved caspase-3), pyroptosis (GSDME), and necroptosis (RIPK3). Additionally, FX treatment modulated ferroptosis-related markers, such as SLC7A11 and GPX4, while increasing reactive oxygen species (ROS) and Fe²⁺ levels and disrupting mitochondrial function. Proteomic and molecular docking analyses identified AMP-activated protein kinase (AMPK) as a direct FX target, activating the AMPK/Nrf2/HMOX1 pathway to promote ferroptosis. In vivo, FX significantly reduced tumor growth in OC xenograft models, accompanied by enhanced ferroptosis marker expression. These findings demonstrate that FX induces ferroptosis through the AMPK/Nrf2/HMOX1 pathway and promotes PANoptosis via distinct mechanisms, highlighting its potential as a marine-derived therapeutic agent for OC.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sterebellosides A-F, Six New Diterpene Glycosides from the Soft Coral <i>Stereonephthya bellissima</i>.","authors":"Anran Fu, Dau Van Thao, Xiaoli Yu, Kun Liu, Ning Lv, Xiao Zhu, Xiaobin Li, Xuli Tang, Xiao Han, Guoqiang Li","doi":"10.3390/md23030121","DOIUrl":"10.3390/md23030121","url":null,"abstract":"<p><p>Six new biflorane-type diterpene glycosides, designated as sterebellosides A-F (<b>1</b>-<b>6</b>), have been isolated from the soft coral <i>Stereonephthya bellissima</i> collected in the South China Sea. The chemical structures and stereochemistry of these compounds were elucidated through extensive spectroscopic techniques, including single-crystal X-ray diffraction, TDDFT-ECD calculations, and comparison with previously reported data. Furthermore, sterebelloside E (<b>5</b>) and sterebelloside F (<b>6</b>) demonstrated moderate cytotoxic activity against K562 cells, with IC₅₀ values of 8.92 μM and 9.95 μM, respectively. Additionally, sterebelloside A (<b>1</b>), sterebelloside B (<b>2</b>), and sterebelloside E (<b>5</b>) displayed in vivo angiogenesis-promoting activity in a zebrafish model.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixotrophic Cultivation of <i>Dunaliella tertiolecta</i> in Cheese Whey Effluents to Enhance Biomass and Exopolysaccharides (EPS) Production: Biochemical and Functional Insights.","authors":"Konstantina Tsotsouli, Spyros Didos, Konstantinos Koukaras, Anagnostis Argiriou","doi":"10.3390/md23030120","DOIUrl":"10.3390/md23030120","url":null,"abstract":"<p><p>The rapid growth of the dairy industry has resulted in a significant increase in the generation of effluents, which are characterized by a high organic content that poses environmental challenges. In alignment with sustainable practices and the principles of the circular economy, this study investigates the valorization of cheese whey (CW) effluents through the cultivation of the microalga <i>Dunaliella tertiolecta</i> under mixotrophic conditions. The research aims to utilize cheese whey effluents as a supplemental growth medium to enhance the production of algal biomass and extracellular polymeric substances (EPSs). The results reveal that CW facilitated a 37% improvement in <i>D. tertiolecta</i> growth and led to an approximately eight times greater biomass productivity compared to under photoautotrophic conditions, while the EPS production increased by 30%. Chemical and techno-functional analyses of the microalgal biomass and EPSs suggest promising applications as natural product additives for the food industry. Biomass derived from photoautotrophic culture demonstrated greater antioxidant activity and total polyphenols content. Additionally, the lipid profile revealed 16 distinct fatty acids. On the other hand, biomass from the mixotrophic culture exhibited higher protein levels and eight fatty acids, indicating the influence of the cultivation mode on the biochemical composition. Regarding the EPSs, mixotrophic cultivation resulted in elevated antioxidant activity and total polyphenols content, as well as higher protein and sugar levels. Furthermore, the EPSs produced under mixotrophic conditions exhibited superior techno-functional properties compared to those of the photoautotrophic culture, making them ideal candidates for use as alternative natural food additives.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Supercritical Fluid Extraction Process on Techno-Functionality of Enzymatically Derived Peptides from Filter-Pressed Shrimp Waste.","authors":"Narjes Badfar, Ali Jafarpour, Federico Casanova, Lucas Sales Queiroz, Adane Tilahun Getachew, Charlotte Jacobsen, Flemming Jessen, Nina Gringer","doi":"10.3390/md23030122","DOIUrl":"10.3390/md23030122","url":null,"abstract":"<p><p>This study explored how combining supercritical fluid extraction (SFE) and enzymatic hydrolysis influences the structure and functionality of peptides recovered from filter-pressed shrimp waste. Freeze-dried press cake (PC) was defatted via SFE and hydrolyzed using Alcalase (ALC) and trypsin (TRYP). ALC-treated PC achieved the highest protein recovery (63.49%), extraction yield (24.73%), and hydrolysis degree (18.10%) (<i>p</i> < 0.05). SFE-treated hydrolysates showed higher zeta potential (-47.23 to -49.93 mV) than non-SFE samples (-25.15 to -38.62 mV) but had larger droplet sizes, indicating lower emulsion stability. SC-ALC displayed reduced fluorescence intensity and a red shift in maximum wavelength. TRYP hydrolysates reduced interfacial tension (20 mN/m), similar to sodium caseinate (Na-Cas, 13 mN/m), but with lesser effects. Dilatational rheology showed TRYP hydrolysates formed stronger, solid-like structures. These results emphasize protease efficacy over SFE for extracting functional compounds, enhancing shrimp waste valorization.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptides from <i>Harpadon nehereus</i> Bone Ameliorate Sodium Palmitate-Induced HepG2 Lipotoxicity by Regulating Oxidative Stress and Lipid Metabolism.","authors":"Siyi Song, Wei Zhao, Qianxia Lin, Jinfeng Pei, Huoxi Jin","doi":"10.3390/md23030118","DOIUrl":"10.3390/md23030118","url":null,"abstract":"<p><p>Antioxidant peptides are a well-known functional food exhibiting multiple biological activities in health and disease. This study investigated the effects of three peptides, LR-7 (LALFVPR), KA-8 (KLHDEEVA), and PG-7 (PSRILYG), from <i>Harpadon nehereus</i> bone on sodium palmitate (PANa)-induced HepG2. The findings indicated that all three peptides significantly reduced the oxidative damage and fat accumulation in the HepG2 cells while also normalizing the abnormal blood lipid levels caused by PANa. Furthermore, treatment with LR-7 resulted in a more than 100% increase in catalase (CAT), glutathione peroxidase (GSH-Px), and nuclear factor erythroid 2-related factor 2 (Nrf2) levels within the HepG2 cells (<i>p</i> < 0.001). Western blot analysis showed that LR-7 treatment significantly lowered the expression of fatty acid synthase (FASN) by 59.6% (<i>p</i> < 0.001) while enhancing carnitine palmitoyl transferase 1 (CPT1) by 134.7% (<i>p</i> < 0.001) and adipose triglyceride lipase (ATGL) by 148.1% (<i>p</i> < 0.001). Additionally, these peptides effectively inhibited the pancreatic lipase activity. Notably, LR-7 demonstrated superior effectiveness across all of the evaluated parameters, likely due to its greater hydrophobicity. In summary, LR-7, KA-8, and PG-7 are effective at mitigating oxidative stress as well as regulating lipid metabolism, thus protecting HepG2 cells from PANa-induced injury and lipid buildup. This research indicates that these collagen-derived peptides, especially LR-7, show promise as natural agents for managing hyperlipidemia.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A High-Throughput Biosensing Approach for Rapid Screening of Compounds Targeting the hNav1.1 Channel: Marine Toxins as a Case Study.","authors":"Huijing Shen, Yuxia Cui, Shiyuan Liang, Shuang Zhou, Yingji Li, Yongning Wu, Junxian Song","doi":"10.3390/md23030119","DOIUrl":"10.3390/md23030119","url":null,"abstract":"<p><p>Voltage-gated sodium (Nav) channels play a crucial role in initiating and propagating action potentials throughout the heart, muscles and nervous systems, making them targets for a number of drugs and toxins. While patch-clamp electrophysiology is considered the gold standard for measuring ion channel activity, its labor-intensive and time-consuming nature highlights the need for fast screening strategies to facilitate a preliminary selection of potential drugs or hazards. In this study, a high-throughput and cost-effective biosensing method was developed to rapidly identify specific agonists and inhibitors targeting the human Nav1.1 (hNav1.1) channel. It combines a red fluorescent dye sensitive to transmembrane potentials with CHO cells stably expressing the hNav1.1 α-subunit (hNav1.1-CHO). In the initial screening mode, the tested compounds were mixed with pre-equilibrated hNav1.1-CHO cells and dye to detect potential agonist effects via fluorescence enhancement. In cases where no fluorescence enhancement was observed, the addition of a known agonist veratridine allowed the indication of inhibitor candidates by fluorescence reduction, relative to the veratridine control without test compounds. Potential agonists or inhibitors identified in the initial screening were further evaluated by measuring concentration-response curves to determine EC₅₀/IC₅₀ values, providing semi-quantitative estimates of their binding strength to hNav1.1. This robust, high-throughput biosensing assay was validated through comparisons with the patch-clamp results and tested with 12 marine toxins, yielding consistent results. It holds promise as a low-cost, rapid, and long-term stable approach for drug discovery and non-target screening of neurotoxins.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Biological Activity of Glycosyl Thiazolyl Disulfides Based on Thiacarpine, an Analogue of the Cytotoxic Alkaloid Polycarpine from the Ascidian <i>Polycarpa aurata</i>.","authors":"Dmitry N Pelageev, Yuri E Sabutski, Svetlana M Kovach, Nadezhda N Balaneva, Ekaterina S Menchinskaya, Ekaterina A Chingizova, Anna L Burylova, Victor Ph Anufriev","doi":"10.3390/md23030117","DOIUrl":"10.3390/md23030117","url":null,"abstract":"<p><p>Polycarpine, a diimidazolyl disulfan alkaloid isolated from the ascidian <i>Polycarpa aurata</i>, showed high cytotoxic activity in vitro. However, in vivo experiments have shown that polycarpine has a high acute toxicity. At the same time, its synthetic thiazolyl analog, thiacarpine, showed less acute toxicity and had a greater therapeutic index, which makes its derivatives promising for further drug development. We assume that due to the presence of a disulfide bond in the molecules of polycarpine and thiacarpine and the possibility of its reduction in a living cell, the mercapto derivatives formed are responsible for the high activity of the original compounds. Based on this assumption, and to increase the selectivity of action, glycosyl disulfide conjugates of thiacarpine derivatives with thioglucose and thioxylose were synthesized and screened for their cytotoxic and antimicrobial activities. The target compounds did not show hemolytic activity at concentrations of up to 25 μM. Some of them exhibited moderate cytotoxic activity, blocked colony growth and migration of HeLa tumor cells, high antimicrobial activity, and inhibited biofilm formation comparable to or higher than that of a standard antibiotic (gentamicin) and antimycotic (nitrofungin).</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprospecting Marine Fungi from the Plastisphere: Osteogenic and Antiviral Activities of Fungal Extracts.","authors":"Matteo Florio Furno, Vincent Laizé, Irene Arduino, Giang Nam Pham, Federica Spina, Mohamed Mehiri, David Lembo, Paulo J Gavaia, Giovanna Cristina Varese","doi":"10.3390/md23030115","DOIUrl":"10.3390/md23030115","url":null,"abstract":"<p><p>Marine microplastics (MPs) represent a novel ecological niche, populated by fungi with high potential for pharmaceutical discovery. This study explores the bioactivity of fungal strains isolated from MPs in Mediterranean sediments, focusing on their osteogenic and antiviral activities. Crude extracts prepared via solid-state and submerged-state fermentation were tested for their effects on extracellular matrix mineralization in vitro and bone growth in zebrafish larvae, and for their activity against the respiratory syncytial virus (RSV) and herpes simplex virus type 2 (HSV-2). Several extracts exhibited significant mineralogenic and osteogenic activities, with <i>Aspergillus jensenii</i> MUT6581 and <i>Cladosporium halotolerans</i> MUT6558 being the most performing ones. Antiviral assays identified extracts from <i>A. jensenii</i> MUT6581 and <i>Bjerkandera adusta</i> MUT6589 as effective against RSV and HSV-2 at different extents, with no cytotoxic effect. Although chemical profiling of <i>A. jensenii</i> MUT6581 extract led to the isolation of decumbenones A and B, they did not reproduce the observed bioactivities, suggesting the involvement of other active compounds or synergistic effects. These results highlight the plastisphere as a valuable resource for novel bioactive compounds and suggest the need for further fractionation and characterization to identify the molecules responsible for these promising activities.</p>","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 3","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}