Marine Drugs最新文献_第2页

Marmaricines A-C: Antimicrobial Brominated Pyrrole Alkaloids from the Red Sea Marine Sponge Agelas sp. aff. marmarica.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-12 DOI: 10.3390/md23020080

Diaa T A Youssef, Areej S Alqarni, Ameen M Almohammadi, Turki Abujamel, Lamiaa A Shaala

{"title":"Marmaricines A-C: Antimicrobial Brominated Pyrrole Alkaloids from the Red Sea Marine Sponge Agelas sp. aff. marmarica.","authors":"Diaa T A Youssef, Areej S Alqarni, Ameen M Almohammadi, Turki Abujamel, Lamiaa A Shaala","doi":"10.3390/md23020080","DOIUrl":"10.3390/md23020080","url":null,"abstract":"The Red Sea is the home of a rich diversity of sponge species with unique ecological adaptations that thrive in its saline, warm, and nutrient-poor waters. Red Sea sponges offer potential as sources of bioactive compounds and novel drugs. The organic extract of the Red Sea sponge Agelas sp. aff. marmarica was investigated for its antimicrobial constituents. Through bioassay-guided fractionation of the antimicrobial fraction of the extract on SiO2 and Sephadex LH-20, as well as HPLC purification, three bioactive compounds, marmaricines A-C (1-3), were isolated. Structural elucidation of the compounds was performed using 1D (1H and 13C) and 2D (COSY, HSQC, HMBC, and NOESY) NMR, as well as (+)-HRESIMS, leading to the identification of the compounds. The antimicrobial activities of the compounds were assessed through evaluation of their inhibition zones, MIC, MBC, and MFC, against Methicillin-Resistant Staphylococcus aureus (MRSA), Escherichia coli, and Candida albicans. Marmaricines A and B exhibited the strongest antibacterial effects against MRSA, with inhibition zones ranging from 14.00 to 15.00 mm, MIC values of 8 µg/mL, and MBC values of 16 µg/mL. In comparison, marmaracine C showed slightly weaker activity (inhibition zone: 12 mm, MIC: 16 µg/mL, MBC: 32 µg/mL). In terms of antifungal activity, marmaricines B and C demonstrated the greatest effect against C. albicans, with inhibition zones of 14-15 mm, MIC values of 8 µg/mL, and MFCs of 16 µg/mL. Interestingly, none of the compounds showed any inhibitory effect against E. coli. The results indicate that marmaricines A-C are selectively active against MRSA, and marmaricines B and C demonstrate potential against C. albicans, making them promising candidates for the development of novel antimicrobial agents targeting resistant pathogens.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic Diversity of the Sea Anemone Actinia fragacea: Comparative Analysis of Nematocyst Venom, Mucus, and Tissue-Specific Profiles.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-11 DOI: 10.3390/md23020079

Ricardo Alexandre Barroso, Tomás Rodrigues, Alexandre Campos, Daniela Almeida, Francisco A Guardiola, Maria V Turkina, Agostinho Antunes

{"title":"Proteomic Diversity of the Sea Anemone Actinia fragacea: Comparative Analysis of Nematocyst Venom, Mucus, and Tissue-Specific Profiles.","authors":"Ricardo Alexandre Barroso, Tomás Rodrigues, Alexandre Campos, Daniela Almeida, Francisco A Guardiola, Maria V Turkina, Agostinho Antunes","doi":"10.3390/md23020079","DOIUrl":"10.3390/md23020079","url":null,"abstract":"Sea anemones (Actiniaria, Cnidaria) are promising targets for biomedical research, as they produce unique bioactive compounds, including toxins and antimicrobial peptides (AMPs). However, the diversity and mechanisms underlying their chemical defenses remain poorly understood. In this study, we investigate the proteomic profiles of the unexplored sea anemone Actinia fragacea by analyzing its venom nematocyst extract, tissues, and mucus secretion. A total of 4011 different proteins were identified, clustered into 3383 protein groups. Among the 83 putative toxins detected, actinoporins, neurotoxins, and phospholipase A2 were uncovered, as well as two novel zinc metalloproteinases with two specific domains (ShK) associated with potassium channel inhibition. Common Gene Ontology (GO) terms were related to immune responses, cell adhesion, protease inhibition, and tissue regeneration. Furthermore, 1406 of the 13,276 distinct peptides identified were predicted as potential AMPs, including a putative Aurelin-like AMP localized within the nematocysts. This discovery highlights and strengthens the evidence for a cnidarian-exclusive Aurelin peptide family. Several other bioactive compounds with distinctive defense functions were also detected, including enzymes, pattern recognition proteins (PRPs), and neuropeptides. This study provides the first proteome map of A. fragacea, offering a critical foundation for exploring novel bioactive compounds and valuable insights into its molecular complexity.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geliboluols A-D: Kaurane-Type Diterpenoids from the Marine-Derived Rare Actinomycete Actinomadura geliboluensis.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-10 DOI: 10.3390/md23020078

Chang-Su Heo, Jong Soon Kang, Jeong-Wook Yang, Min Ah Lee, Hwa-Sun Lee, Chang Hwan Kim, Hee Jae Shin

引用次数: 0

Wound Healing, Antioxidant, and Antiviral Properties of Bioactive Polysaccharides of Microalgae Strains Isolated from Greek Coastal Lagoons.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-10 DOI: 10.3390/md23020077

Gabriel Vasilakis, Sofia Marka, Alexandros Ntzouvaras, Maria-Eleftheria Zografaki, Eirini Kyriakopoulou, Katerina I Kalliampakou, Georgios Bekiaris, Evangelos Korakidis, Niki Papageorgiou, Stefania Christofi, Niki Vassilaki, Georgia Moschopoulou, Ioannis Tzovenis, Athena Economou-Amilli, Seraphim Papanikolaou, Emmanouil Flemetakis

{"title":"Wound Healing, Antioxidant, and Antiviral Properties of Bioactive Polysaccharides of Microalgae Strains Isolated from Greek Coastal Lagoons.","authors":"Gabriel Vasilakis, Sofia Marka, Alexandros Ntzouvaras, Maria-Eleftheria Zografaki, Eirini Kyriakopoulou, Katerina I Kalliampakou, Georgios Bekiaris, Evangelos Korakidis, Niki Papageorgiou, Stefania Christofi, Niki Vassilaki, Georgia Moschopoulou, Ioannis Tzovenis, Athena Economou-Amilli, Seraphim Papanikolaou, Emmanouil Flemetakis","doi":"10.3390/md23020077","DOIUrl":"10.3390/md23020077","url":null,"abstract":"Microalgae have gained significant attention as sustainable sources of high value compounds, such as bioactive polysaccharides that are usually rich in sulfated groups and exhibit antioxidant properties. Here, 14 new microalgae strains of the genera Tetraselmis, Dunaliella, and Nannochloropsis, isolated from Greek coastal lagoons were analyzed to quantify and characterize their polysaccharide content. Heterogeneity was observed regarding the content of their total sugars (5.5-40.9 g/100 g dry biomass). The strains with a total sugar content above 20% were analyzed concerning the content of total, α- and β-glucans. Tetraselmis verrucosa f. rubens PLA1-2 and T. suecica T3-1 were rich in β-glucans (11%, and 8.1%, respectively). The polysaccharides of the two Tetraselmis strains were isolated and they were mainly composed of glucose and galactose. The isolated polysaccharides were fractionated using ion-exchange-chromatography. The anionic fraction from T. verrucosa f. rubens PLA1-2 was rich in sulfated polysaccharides, had antioxidant capacity, and exhibited healing properties. The anionic polysaccharides from the two Tetraselmis strains did not negatively influence the viability of human cells, while exhibiting antiviral properties against the replication of Hepatitis C Virus (HCV), with median efficient concentrations (EC50) at a range of 210-258 μg/mL.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of Transcriptomics and Proteomics to Elucidate Inhibitory Effect and Mechanism of Antifungalmycin B from Marine Streptomyces hiroshimensis in Treating Talaromyces marneffei.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-10 DOI: 10.3390/md23020076

Qiqi Li, Zhou Wang, Cuiping Jiang, Jianglin Yin, Yonghong Liu, Xinjian Qu, Xiangxi Yi, Chenghai Gao

{"title":"Integration of Transcriptomics and Proteomics to Elucidate Inhibitory Effect and Mechanism of Antifungalmycin B from Marine Streptomyces hiroshimensis in Treating Talaromyces marneffei.","authors":"Qiqi Li, Zhou Wang, Cuiping Jiang, Jianglin Yin, Yonghong Liu, Xinjian Qu, Xiangxi Yi, Chenghai Gao","doi":"10.3390/md23020076","DOIUrl":"10.3390/md23020076","url":null,"abstract":"Talaromyces marneffei (TM) is an opportunistic pathogenic fungus that mainly infects immunocompromised patients. Currently, the global prevalence of talaromycosis caused by TM is increasing, leading to an increased demand for anti-TM drugs. In our previous study, a novel 28-membered macrolide compound, antifungalmycin B (ANB), was isolated from Streptomyces hiroshimensis GXIMD 06359, exhibiting significant antifungal properties. However, its in vivo mechanisms and direct antifungal effects warrant further investigation. In this study, we employed a mouse model in conjunction with transcriptomic and proteomic approaches to explore the antifungal activity of ANB against T. marneffei. In an in vivo mouse model infected with T. marneffei infection, ANB significantly reduced fungal burdens in the liver, spleen, lungs, and kidneys. Additionally, it markedly decreased the levels of reactive oxygen species (ROS) and cytokines, including interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. Proteomic and transcriptomic studies, complemented by parallel reaction monitoring (PRM) analysis, revealed that ANB effectively disrupted acid biosynthesis and cellular energy metabolism, thereby impairing mitochondrial functions in T. marneffei. These effects were exerted through multiple pathways. These findings highlight the potential of ANB as a versatile inhibitor of polyene macrolide-resistant fungi, offering a promising therapeutic avenue for the treatment of talaromycosis.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of Acetylcholinesterase Inhibitory Peptides from Yellowfin Tuna Pancreas Using Moderate Ultrasound-Assisted Enzymatic Hydrolysis.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-09 DOI: 10.3390/md23020075

Pai Peng, Hui Yu, Meiting Xian, Caiye Qu, Zhiqiang Guo, Shuyi Li, Zhenzhou Zhu, Juan Xiao

{"title":"Preparation of Acetylcholinesterase Inhibitory Peptides from Yellowfin Tuna Pancreas Using Moderate Ultrasound-Assisted Enzymatic Hydrolysis.","authors":"Pai Peng, Hui Yu, Meiting Xian, Caiye Qu, Zhiqiang Guo, Shuyi Li, Zhenzhou Zhu, Juan Xiao","doi":"10.3390/md23020075","DOIUrl":"10.3390/md23020075","url":null,"abstract":"Bioactive peptides represent a promising therapeutic approach for Alzheimer's disease (AD) by maintaining cholinergic system homeostasis through the inhibition of acetylcholinesterase (AChE) activity. This study focused on extracting AChE inhibitory peptides from yellowfin tuna pancreas using moderate ultrasound-assisted enzymatic hydrolysis (MUE). Firstly, papain and MUE stood out from five enzymes and four enzymatic hydrolysis methods, respectively, by comparing the degree of hydrolysis and AChE inhibitory activity of different pancreatic protein hydrolysates. Subsequently, the optimal MUE conditions were obtained by single-factor, Plackett-Burman, and response surface methodologies. The pancreatic protein hydrolysate prepared under optimal MUE conditions was then purified by ultrafiltration followed by RP-HPLC, from which a novel AChE inhibitory peptide (LLDF) was identified by LC-MS/MS and virtual screening. LLDF effectively inhibited AChE activity by a competitive inhibition mechanism, with an IC50 of 18.44 ± 0.24 μM. Molecular docking and molecular dynamic simulation revealed that LLDF bound robustly to the active site of AChE via hydrogen bonds. These findings provided a theoretical basis for the valuable use of yellowfin tuna pancreas and introduced a new viewpoint on the potential therapeutic advantages of AChE inhibitory peptides for future AD treatment.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced Technologies for Large Scale Supply of Marine Drugs.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-07 DOI: 10.3390/md23020069

Henar Martínez, Mercedes Santos, Lucía Pedraza, Ana M Testera

{"title":"Advanced Technologies for Large Scale Supply of Marine Drugs.","authors":"Henar Martínez, Mercedes Santos, Lucía Pedraza, Ana M Testera","doi":"10.3390/md23020069","DOIUrl":"10.3390/md23020069","url":null,"abstract":"Marine organisms represent a source of unique chemical entities with valuable biomedical potentialities, broad diversity, and complexity. It is essential to ensure a reliable and sustainable supply of marine natural products (MNPs) for their translation into commercial drugs and other valuable products. From a structural point of view and with few exceptions, MNPs of pharmaceutical importance derive from the so-called secondary metabolism of marine organisms. When production strategies rely on marine macroorganisms, harvesting or culturing coupled with extraction procedures frequently remain the only alternative to producing these compounds on an industrial scale. Their supply can often be implemented with laboratory scale cultures for bacterial, fungal, or microalgal sources. However, a diverse approach, combining traditional methods with modern synthetic biology and biosynthesis strategies, must be considered for invertebrate MNPs, as they are usually naturally accumulated in only very small quantities. This review offers a comprehensive examination of various production strategies for MNPs, addressing the challenges related to supply, synthesis, and scalability. It also underscores recent biotechnological advancements that are likely to transform the current industrial-scale manufacturing methods for pharmaceuticals derived from marine sources.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer Properties of Macroalgae: A Comprehensive Review.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-07 DOI: 10.3390/md23020070

Sara Frazzini, Luciana Rossi

{"title":"Anticancer Properties of Macroalgae: A Comprehensive Review.","authors":"Sara Frazzini, Luciana Rossi","doi":"10.3390/md23020070","DOIUrl":"10.3390/md23020070","url":null,"abstract":"In recent years, the exploration of bioactive molecules derived from natural sources has gained interest in several application fields. Among these, macroalgae have garnered significant attention due to their functional properties, which make them interesting in therapeutic applications, including cancer treatment. Cancer constitutes a significant global health burden, and the side effects of existing treatment modalities underscore the necessity for the exploration of novel therapeutic models that, in line with the goal of reducing drug treatments, take advantage of natural compounds. This review explores the anticancer properties of macroalgae, focusing on their bioactive compounds and mechanisms of action. The key findings suggest that macroalgae possess a rich array of bioactive compounds, including polysaccharides (e.g., fucoidans and alginates), polyphenols (e.g., phlorotannins), and terpenoids, which exhibit diverse anticancer activities, such as the inhibition of cell proliferation, angiogenesis, induction of apoptosis, and modulation of the immune system. This review provides an overview of the current understanding of macroalgae's anticancer potential, highlighting the most promising compounds and their mechanisms of action. While preclinical studies have shown promising results, further research is necessary to translate these findings into effective clinical applications.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TMF Attenuates Cognitive Impairment and Neuroinflammation by Inhibiting the MAPK/NF-κB Pathway in Alzheimer's Disease: A Multi-Omics Analysis.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-07 DOI: 10.3390/md23020074

Yonglin Liu, Xi Xu, Xiaoming Wu, Guodong Yang, Jiaxin Luo, Xinli Liang, Jie Chen, Yiguang Li

引用次数: 0

Marine-Derived Diterpenes from 2019 to 2024: Structures, Biological Activities, Synthesis and Potential Applications.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-07 DOI: 10.3390/md23020072

Lin Zhang, Debao Li, Xuan Chen, Feng Zhao

{"title":"Marine-Derived Diterpenes from 2019 to 2024: Structures, Biological Activities, Synthesis and Potential Applications.","authors":"Lin Zhang, Debao Li, Xuan Chen, Feng Zhao","doi":"10.3390/md23020072","DOIUrl":"10.3390/md23020072","url":null,"abstract":"Marine diterpenes are an important category of secondary metabolites derived from marine sources, found in a variety of marine animals, plants, and fungi. The increasing diversity of diterpene compounds, along with their unique chemical structures and specific biological activities, have attracted widespread attention. These activities include anti-inflammatory, antiviral, antitumor, antibacterial effects, and therapeutic actions against cancer, with many already developed into clinical drugs. Additionally, as marine medicinal resources continue to be exploited over the long term, the natural resources of many marine diterpene compounds are diminishing, and the biosynthesis of key active components has become a hot topic of research. In this review, we summarize diterpene compounds discovered in the ocean over the past five years, reclassify these compounds, and summarize their structures, biological activities, biosynthesis, and potential applications of marine diterpenoids from 2019 to 2024. This review can provide a reference for the basic research and potential applications of marine-derived diterpene compounds.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0