Journal of Reproduction and Development最新文献_第7页

Controlled internal drug release (CIDR) removal before luteolysis prolongs estrus interval and increases silent heat risk in Japanese Black cows. 黄体溶解前控释内源性药物（CIDR）延长了日本黑牛的发情间隔，增加了静热风险。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-04-15 Epub Date: 2026-02-19 DOI: 10.1262/jrd.2025-076

Takuo Hojo, Kei Horihata, Naoki Takenouchi

{"title":"Controlled internal drug release (CIDR) removal before luteolysis prolongs estrus interval and increases silent heat risk in Japanese Black cows.","authors":"Takuo Hojo, Kei Horihata, Naoki Takenouchi","doi":"10.1262/jrd.2025-076","DOIUrl":"10.1262/jrd.2025-076","url":null,"abstract":"This study aimed to improve estrus management in Japanese Black cows by investigating the effects of controlled internal drug release (CIDR) treatment on luteal function including luteolysis and estrous characteristics. Cows with normal estrous cycles were divided into three groups, and CIDR treatment was initiated on day 5 (Group 1), 10 (Group 2), or 16 (Group 3) of the estrous cycle and continued for 12 days. Plasma progesterone (P4) levels, luteal diameter, and luteal blood flow (LBF) were monitored during treatment, and correlations among these parameters were analyzed. Additionally, the interval from CIDR removal to estrus, and the incidence of standing (ST) behavior during estrus were evaluated. All luteal parameters changed in a cycle-dependent manner, irrespective of CIDR treatment. Significant correlations were observed between P4 levels and both luteal diameter and LBF. The interval from treatment withdrawal to estrus was significantly longer in Group 1 (4.6 ± 2.2 days) than in Groups 2 (2.1 ± 0.4 days) and 3 (2.2 ± 0.7 days). Silent heat, defined as estrus without ST behavior, occurred in 27.3% of cows in group 1 and 10.0% in Group 2, but not in Group 3. These findings suggest that initiating CIDR treatment in the early luteal stage and removing it just before or during luteolysis, causes variability in estrus timing after treatment and increases the risk of silent heat. When implementing estrus synchronization using CIDR in the field, both the timing of treatment initiation and removal should be carefully considered.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"65-72"},"PeriodicalIF":2.1,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13078928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of timing and morphology of blastomere cleavage on gene expression profiles of bovine in vitro fertilized embryos. 卵裂时间和形态对牛体外受精胚胎基因表达谱的影响。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2026-02-17 Epub Date: 2025-12-13 DOI: 10.1262/jrd.2025-035

Shunki Ono, Hinano Kozuka, Kota Okubo, Takashi Fujii, Ken Sawai

引用次数: 0

Transgenic Bax gene efficiently induces lethality in mouse early embryos. 转Bax基因可有效诱导小鼠早期胚胎致死性。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2026-02-17 Epub Date: 2025-12-20 DOI: 10.1262/jrd.2025-054

Yuzuki Goto, Takuto Yamamoto, Masahiro Sakata, Satoshi Mashiko, Daiki Shikata, Shinnosuke Honda, Naojiro Minami, Shuntaro Ikeda

{"title":"Transgenic Bax gene efficiently induces lethality in mouse early embryos.","authors":"Yuzuki Goto, Takuto Yamamoto, Masahiro Sakata, Satoshi Mashiko, Daiki Shikata, Shinnosuke Honda, Naojiro Minami, Shuntaro Ikeda","doi":"10.1262/jrd.2025-054","DOIUrl":"10.1262/jrd.2025-054","url":null,"abstract":"Apoptosis is an essential physiological process involved in embryonic development, immune responses, and tissue homeostasis. Despite many studies on pro-apoptotic genes, few reports have directly compared the lethality-inducing potential between them under comparable conditions. In this study, we evaluated the lethality-inducing potential of three representative pro-apoptotic genes, Bax, Casp3, and Casp9, in mouse early embryos under defined conditions using the doxycycline (Dox)-inducible tetracycline-regulated gene expression system in combination with the PiggyBac transposon system. All genes were transcriptionally induced by Dox, and Bax showed the strongest lethal effect, followed by Casp9, while Casp3 did not show any effects. Notably, Bax expression severely impaired blastocyst formation and led to the intense accumulation of the DNA damage marker γH2AX, along with a pronounced increase in the apoptotic cells. These findings suggest that introducing upstream apoptotic regulators leads to the more efficient and widespread activation of the apoptotic cascade. Overall, this study is expected to contribute to a deeper understanding of apoptotic mechanisms and future advancements in regenerative medicine, reproductive engineering, and cancer research.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"16-23"},"PeriodicalIF":2.2,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATAD2 deficiency leads to subfertility by impairing spermatogenesis in mice. 在小鼠中，ATAD2缺乏通过损害精子发生导致生育能力低下。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2026-02-17 Epub Date: 2025-12-25 DOI: 10.1262/jrd.2025-060

Rui Wu, Shuyun Zhao, Qi Yu, Yili Zhao, Yan Han, Rong Liu, ChaoQun Duan

{"title":"ATAD2 deficiency leads to subfertility by impairing spermatogenesis in mice.","authors":"Rui Wu, Shuyun Zhao, Qi Yu, Yili Zhao, Yan Han, Rong Liu, ChaoQun Duan","doi":"10.1262/jrd.2025-060","DOIUrl":"10.1262/jrd.2025-060","url":null,"abstract":"During spermatogenesis, chromatin remodeling regulated by histone modification is essential for spermatogenic cell development, and multiple epigenetic regulators are involved in this process. Recent studies reported that ATAD2 was a newly discovered cancer/testis factor that functioned in chromatin remodeling in somatic cells by binding acetylated histone. However, the physiological role of ATAD2 in spermatogenesis is largely unknown. In this study, we characterized the expression pattern of ATAD2 in mouse testes and found that the highly expressed ATAD2 in the gonads was lowly expressed in meiotic spermatocytes with distinct localization in nucleus, but highly expressed in round spermatids. By generating Atad2 knockout (KO) mice using CRISPR/Cas9 technology, we revealed that ATAD2 deletion leads to failure of DSB repair and chromosome synapsis in spermatocytes and impairs spermiogenesis in spermatids. Atad2 KO mice were subfertile, as characterized by reduced sperm count, impaired motility, and abnormal morphology. RNA-Seq analysis showed that hundreds of genes were dysregulated in Atad2-KO round spermatids. As revealed by GSEA analysis, the gene set related to spermatid development was downregulated, while gene sets related to chromatin binding and positive and negative DNA-templated transcription were upregulated. In conclusion, our results indicate that ATAD2 contributes to meiotic progression and participates in spermiogenesis by regulating RNA transcription in spermatids.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"24-33"},"PeriodicalIF":2.2,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145827926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of a comprehensive metabolomic analysis system for characterizing metabolic alterations in the cauda epididymis of mature and juvenile mice. 成熟和幼年小鼠附睾尾代谢变化的综合代谢组学分析系统的优化。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2026-02-17 Epub Date: 2026-01-03 DOI: 10.1262/jrd.2025-063

Ryusei Maeda, Satohiro Nakao, Yuichiro Arima, Ayana Shitashimizu, Keisuke Masuda, Naomi Nakagata, Toru Takeo

{"title":"Optimization of a comprehensive metabolomic analysis system for characterizing metabolic alterations in the cauda epididymis of mature and juvenile mice.","authors":"Ryusei Maeda, Satohiro Nakao, Yuichiro Arima, Ayana Shitashimizu, Keisuke Masuda, Naomi Nakagata, Toru Takeo","doi":"10.1262/jrd.2025-063","DOIUrl":"10.1262/jrd.2025-063","url":null,"abstract":"The cauda epididymis protects and stores mature sperm in mammals. Recently, comprehensive transcriptomic and proteomic analyses have been conducted to understand its molecular functions; however, fundamental information on its metabolites has not been reported. In this study, we optimized a system for the comprehensive metabolic analysis of the cauda epididymis in mature and juvenile mice. This system identified 116 and 92 metabolites in mature and juvenile mice, respectively. Comparative analysis revealed that 44 and 13 metabolites were upregulated and downregulated, respectively, in the cauda epididymis of mature and juvenile mice. Based on the identified metabolites, 34 metabolic and unique pathways (mature: four pathways and juvenile: one pathway) were determined. In conclusion, the levels of certain metabolites in the cauda epididymis differed between mature and juvenile mice. These results contribute to understanding of the unique functions of the cauda epididymis based on dynamic changes in metabolites.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"8-15"},"PeriodicalIF":2.2,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of omentin-1 in female reproduction: a critical review. 网膜蛋白-1在女性生殖中的作用综述。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-01-01 DOI: 10.1262/jrd.2025-107

Karolina Kubicka, Natalia Respekta-Długosz, Oliwia Szkraba, Patrycja Kurowska, Agnieszka Rak

{"title":"The role of omentin-1 in female reproduction: a critical review.","authors":"Karolina Kubicka, Natalia Respekta-Długosz, Oliwia Szkraba, Patrycja Kurowska, Agnieszka Rak","doi":"10.1262/jrd.2025-107","DOIUrl":"10.1262/jrd.2025-107","url":null,"abstract":"White adipose tissue is now recognized as an active endocrine organ that secretes numerous bioactive molecules known as adipokines. These proteins regulate essential physiological processes, including energy metabolism, inflammation, and reproduction, while disturbances in their secretion are linked to conditions such as infertility, obesity, and diabetes. Omentin-1, also termed intelectin-1, is a secreted trimeric lectin conserved across mammals. In addition to systemic actions that improve insulin sensitivity, enhance glucose uptake, and suppress oxidative stress and inflammation, omentin-1 exerts important functions within the female reproductive system. It is expressed in the hypothalamus, pituitary gland, ovary, oocyte, and placenta, where it influences cell proliferation, apoptosis, and hormone production. Through these actions, omentin-1 contributes to hormonal homeostasis, follicular development, oocyte maturation, luteal function - integrating energy balance with female fertility. Altered circulating or local levels of omentin-1 have been observed in polycystic ovary syndrome, endometriosis, ovarian and endometrial cancers, preeclampsia, and gestational diabetes. This review aims to summarize current knowledge on omentin-1 expression and function in the female reproductive system within hypothalamus - pituitary - ovary axis and its role in reproductive pathologies.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"72 3","pages":"410-420"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13286659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148271994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biobanking biodiversity: recent strategies, challenges, and opportunities. 生物银行生物多样性：最近的战略、挑战和机遇。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-01-01 DOI: 10.1262/jrd.2025-114

Pei-Chih Lee, Pierre Comizzoli

{"title":"Biobanking biodiversity: recent strategies, challenges, and opportunities.","authors":"Pei-Chih Lee, Pierre Comizzoli","doi":"10.1262/jrd.2025-114","DOIUrl":"10.1262/jrd.2025-114","url":null,"abstract":"Earth is undergoing accelerated loss of biodiversity. As wild animal populations decline, the resulting erosion of genetic diversity threatens species' adaptive potential and long-term survival. Wildlife biobanking, defined as the systematic collection, preservation, and use of biological materials and their associated metadata, has emerged as a critical conservation strategy. Importantly, building on decades of progress in cryobiology and reproductive biotechnologies, gonadal and somatic tissues and cells from thousands of wild species have been preserved worldwide. These biobanks serve as genetic insurance policies, supporting animal care and management, research, assisted reproduction, and eventual population recovery. This review discusses current strategies, challenges, and future opportunities in wildlife biobanking. Cryopreservation approaches for different sample types and taxa also are reviewed, emphasizing the challenges of sample availability and species-specific cryotolerance. The objective is also to integrate increasingly large amount of data associated with biological samples (digital biobanking) into conservation efforts. New frontiers in biobanking include innovative ambient-temperature preservation, precision biobanking informed by omics technologies, data interpretation using artificial intelligence, and establishment of global networks. These promising developments provide more opportunities to enhance and expand wildlife biobanking more quickly and broadly.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"72 3","pages":"210-224"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13286723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148271953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examining a central mechanism whereby undernutrition inhibits GnRH secretion via KNDy neurons. 研究营养不良通过KNDy神经元抑制GnRH分泌的中枢机制。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-01-01 DOI: 10.1262/jrd.2026-007

Casey C Nestor, Arion Kennedy

引用次数: 0

The role of epigenetic alterations in canine mammary cancer. 表观遗传改变在犬乳腺癌中的作用。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-01-01 DOI: 10.1262/jrd.2025-132

Danilo DO Rosário Pinheiro, Débora Mendes DE Andrade, Nayara Cristina Lima DE Oliveira, Bárbara DO Nascimento Borges

引用次数: 0

Manipulating primordial germ cells from chicken and other bird species. 操纵鸡和其他鸟类的原始生殖细胞。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-01-01 DOI: 10.1262/jrd.2025-085

Marcel H Blank, Kennosuke Ichikawa, Mike J McGrew

引用次数: 0