A polysaccharide gel made of gellan gum improves oocyte maturation and embryonic development in pigs

IF 1.9 4区生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Reproduction and Development Pub Date : 2024-07-13 DOI:10.1262/jrd.2024-017

Shunsuke HARA, Koumei SHIRASUNA, Hisataka IWATA

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Abstract

Gellan gum (GG) is a soft, tractable,　and natural polysaccharide substrate used for cell incubation. In this study, we examined the effects of GG on porcine oocyte maturation. Cumulus cells and oocyte complexes (COCs) were collected from slaughterhouse-derived porcine ovaries and cultured on plastic plates containing 0.05% or 0.1% GG gels. The 0.1% GG gel improved the maturation rate and quality of blastocysts, as determined by the total cell number and the rate of abnormally condensed nuclei. GG gels have antioxidant abilities and oocytes cultured on GG gels (0.05% and 0.1%) have reduced reactive oxygen species (ROS) content. Furthermore, GG gels (0.05% and 0.1%) increased F-actin formation, whereas treatment of oocytes with H₂O₂ reduced F-actin levels. GG gels increased the ATP content in oocytes but did not affect the mitochondrial DNA copy number or mitochondrial membrane potential. In addition, the medium cultured on 0.05% GG increased the glucose consumption of COCs. In conclusion, GG gel reduced ROS content, increased energy content, and improved subsequent embryonic development in pigs.

Graphical Abstract Fullsize Image Graphical Abstract

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由结冷胶制成的多糖凝胶可改善猪的卵母细胞成熟和胚胎发育

结冷胶（GG）是一种用于细胞培养的天然多糖基质，质地柔软，易于处理。在这项研究中，我们考察了结冷胶对猪卵母细胞成熟的影响。我们从屠宰场采集了猪卵巢中的积层细胞和卵母细胞复合体（COCs），并在含有 0.05% 或 0.1% GG 凝胶的塑料板上进行培养。0.1% GG 凝胶提高了囊胚的成熟率和质量，这是由细胞总数和异常凝聚核的比率决定的。GG凝胶具有抗氧化能力，在GG凝胶（0.05% 和 0.1%）上培养的卵母细胞活性氧（ROS）含量降低。此外，麦芽糖凝胶（0.05% 和 0.1%）增加了 F-肌动蛋白的形成，而用 H2O2 处理卵母细胞则降低了 F-肌动蛋白的水平。GG 凝胶增加了卵母细胞中的 ATP 含量，但不影响线粒体 DNA 拷贝数或线粒体膜电位。此外，0.05% GG 培养基增加了 COC 的葡萄糖消耗。总之，GG 凝胶降低了 ROS 含量，增加了能量含量，并改善了猪的后续胚胎发育。图文摘要全尺寸图片图文摘要

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来源期刊

Journal of Reproduction and Development 生物-奶制品与动物科学

CiteScore

3.70

自引率

11.10%

发文量

审稿时长

2 months

期刊介绍： Journal of Reproduction and Development (JRD) is the official journal of the Society for Reproduction and Development, published bimonthly, and welcomes original articles. JRD provides free full-text access of all the published articles on the web. The functions of the journal are managed by Editorial Board Members, such as the Editor-in-Chief, Co-Editor-inChief, Managing Editors and Editors. All manuscripts are peer-reviewed critically by two or more reviewers. Acceptance is based on scientific content and presentation of the materials. The Editors select reviewers and correspond with authors. Final decisions about acceptance or rejection of manuscripts are made by the Editor-in-Chief and Co-Editor-in-Chief.