Journal of Reproduction and Development最新文献

Reduced HSP70 expression under mild hypothermia is involved in bovine embryo growth suppression. 亚低温条件下HSP70表达的降低与牛胚胎生长抑制有关。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-05-11 DOI: 10.1262/jrd.2024-087

Toshimichi Ishii, Sho Nakamura, Satoshi Ohkura, Shuichi Matsuyama

{"title":"Reduced HSP70 expression under mild hypothermia is involved in bovine embryo growth suppression.","authors":"Toshimichi Ishii, Sho Nakamura, Satoshi Ohkura, Shuichi Matsuyama","doi":"10.1262/jrd.2024-087","DOIUrl":"https://doi.org/10.1262/jrd.2024-087","url":null,"abstract":"Bovine blastocysts cultured under mild hypothermia (MH) can be maintained with non-hatching viable embryos compared to normothermic controls (38.5°C). However, the mechanism by which mildly hypothermic culture delays embryonic growth has not yet been elucidated. This study evaluated the number of cells in embryos cultured under MH conditions and the expression of genes involved in embryonic differentiation. Bovine blastocysts cultured under MH conditions exhibited reduced cell numbers and interferon-tau mRNA expression. Both forkhead box O3 (FOXO3) mRNA expression and FOXO3 protein level in blastocysts cultured under MH conditions were higher than those in normothermic controls (P < 0.05). On the phosphorylated FOXO3 protein level, there was no significant difference between blastocysts cultured under MH and normothermic conditions. In contrast, no significant difference was observed in the ATP content of blastocysts between the MH and normothermic groups. In blastocysts cultured under MH conditions, cold-inducible RNA-binding protein (CIRP) and RNA-binding motif protein 3 (RBM3) mRNA expression increased, and heat shock protein 70 (HSP70) mRNA expression decreased compared to that in normothermic controls (P < 0.05). Considering that HSP70 is involved in preventing apoptosis, these results suggest that MH retards embryonic development via apoptosis induced by HSP70 downregulation during the culture period.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro embryo production via ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) in pure and crossbred Japanese Hokkaido native ponies. 通过取卵（OPU）和卵浆内单精子注射（ICSI）在纯种和杂交日本北海道本地小马的体外胚胎生产。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-05-10 DOI: 10.1262/jrd.2025-011

M A Hannan, Hiroyuki Watanabe, Akiko Takeyama, Sakura Yoshida, Dorb Wudamu, Narangerel Lkhagvasuren, Anthony Claes, Tom A E Stout, Soon Hon Cheong, Shingo Haneda, Yasuo Nambo

{"title":"In vitro embryo production via ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) in pure and crossbred Japanese Hokkaido native ponies.","authors":"M A Hannan, Hiroyuki Watanabe, Akiko Takeyama, Sakura Yoshida, Dorb Wudamu, Narangerel Lkhagvasuren, Anthony Claes, Tom A E Stout, Soon Hon Cheong, Shingo Haneda, Yasuo Nambo","doi":"10.1262/jrd.2025-011","DOIUrl":"https://doi.org/10.1262/jrd.2025-011","url":null,"abstract":"This study evaluated the viability of in vitro embryo production using ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) as breeding techniques for pure and crossbred Hokkaido native ponies (n = 9). Oocytes were collected using transvaginal ultrasound-guided follicle aspiration. ICSI was performed on in vitro matured oocytes using frozen semen. Embryonic cultures were monitored using time-lapse cinematography. Blastocysts were cryopreserved and, after thawing, were transferred non-surgically into recipient mares. Over nine OPU sessions, the mean number of aspirated follicles was 23.9 (range, 13-49). The oocyte recovery and maturation rates were 35.3% (76/215) and 61.5% (40/65), respectively. The cleavage rate was 57.5% (23/40). Of cleaved embryos, 56.5% (13/23) were arrested at the 4-cell to 8-cell stage, and five developed into early-blastocyst. Three embryos were transferred, resulting in a successful pregnancy. In conclusion, OPU-ICSI is a viable assisted reproductive technology for enhancing the population of Japanese native horses.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Percentage of follicle number by size over the antral follicle count and its association with subsequent reproductive performance in beef cattle. 肉牛卵泡大小数与窦卵泡数之比及其与后续繁殖性能的关系

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-05-08 DOI: 10.1262/jrd.2024-075

Shiori Saito Kohigashi, Mizuho Uematsu, Go Kitahara, Takeshi Osawa

{"title":"Percentage of follicle number by size over the antral follicle count and its association with subsequent reproductive performance in beef cattle.","authors":"Shiori Saito Kohigashi, Mizuho Uematsu, Go Kitahara, Takeshi Osawa","doi":"10.1262/jrd.2024-075","DOIUrl":"https://doi.org/10.1262/jrd.2024-075","url":null,"abstract":"This study aimed to clarify the association between the percentage of follicle number by size over antral follicle count (AFC) and subsequent reproductive performance. A total of 306 Japanese Black cattle underwent timed artificial insemination (TAI) 41-62 days postpartum; the AFC and numbers of small, medium, and large follicles were recorded 10 days before TAI. The cross-sectional and blood flow areas of the dominant follicle (DF) on the day of TAI and the corpus luteum (CL) six days after TAI were recorded. The total number of follicles ≥ 2 mm was defined as the AFC, and the percentages of follicle number by each size defined as small (S-AFC%; 2-2.9 mm), medium (M-AFC%; 3-8.4 mm), and large (L-AFC%; ≥ 8.5 mm) follicles. The AFC and S-, M-, and L-AFC% were further grouped into low, medium, and high tertiles, and the subsequent reproductive performance compared among the groups. Plasma anti-Müllerian hormone (AMH) levels were quantified on the day of AFC measurement. No differences were observed in reproductive performance between the AFC and L-AFC% groups. The high-S-AFC% group showed a 20.6% lower conception rate, 0.58 more AI numbers, and 21.9 longer days open than those of the low-S-AFC% group (P < 0.05). The low-M-AFC% group showed an 18.0% lower conception rate after TAI and 0.54 more AI numbers than those of the high-M-AFC% group (P < 0.05). DF and CL parameters did not differ among the AFC, S-, M-, and L-AFC% groups. Plasma AMH levels in the low-AFC group were the lowest in the tertile. In conclusion, the percentage of follicles by size could be used to estimate subsequent reproductive performance.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pwp1 inhibition impairs the development and early lineage commitment of mouse preimplantation embryos. Pwp1抑制会损害小鼠植入前胚胎的发育和早期谱系承诺。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-05-03 DOI: 10.1262/jrd.2024-111

Takuto Yamamoto, Atsushi Takasu, Yasuhiro Isumi, Satoshi Mashiko, Daiki Shikata, Shinnosuke Honda, Naojiro Minami, Shuntaro Ikeda

{"title":"Pwp1 inhibition impairs the development and early lineage commitment of mouse preimplantation embryos.","authors":"Takuto Yamamoto, Atsushi Takasu, Yasuhiro Isumi, Satoshi Mashiko, Daiki Shikata, Shinnosuke Honda, Naojiro Minami, Shuntaro Ikeda","doi":"10.1262/jrd.2024-111","DOIUrl":"https://doi.org/10.1262/jrd.2024-111","url":null,"abstract":"During mouse preimplantation development, zygotic genome activation (ZGA), which synthesizes new transcripts in the embryo, occurs during the 1-cell to 2-cell stage. Embryos at the 1- and 2-cell stages are totipotent, and as embryonic development progresses, their differentiation potential decreases, and the embryos become pluripotent. However, the roles of genes expressed during ZGA in mouse embryonic differentiation remain incompletely understood. Here, we show that periodic tryptophan protein 1 (Pwp1), a WD-repeat protein, is expressed from the ZGA and controls embryonic differentiation at later stages. Developmental potential was reduced when siRNAs or antisense oligonucleotides targeting Pwp1 were introduced into 1-cell stage mouse embryos. Further, Pwp1 knockdown resulted in irregular localization of YAP1 at the morula stage, upregulation of the inner cell mass marker Nanog, and downregulation of the trophectoderm marker Cdx2 at the blastocyst stage. Transcriptome analysis showed that Pwp1 knockdown upregulated ZGA gene expression at the morula stage. Because Pwp1 contributes to H4K20me3 histone modification, these results suggest that Pwp1 is required for mouse preimplantation development to control differentiation-associated genes via H4K20me3 modification. Elucidating the role of Pwp1 in embryonic differentiation is expected to contribute toward the advancement of assisted reproductive technologies.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of action of IHH in ameliorating thin endometrium. IHH改善子宫内膜薄的作用机制。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-04-28 DOI: 10.1262/jrd.2024-096

Lan Luo, Man Luo, Donghong Ning, Xi Chen, Qiuman Zheng, Qin Cao

{"title":"Mechanism of action of IHH in ameliorating thin endometrium.","authors":"Lan Luo, Man Luo, Donghong Ning, Xi Chen, Qiuman Zheng, Qin Cao","doi":"10.1262/jrd.2024-096","DOIUrl":"https://doi.org/10.1262/jrd.2024-096","url":null,"abstract":"A thin endometrium can lead to low clinical pregnancy rates, low live birth rates, high spontaneous abortion rates, and low birth weight. However, current methods of treating thin endometria do not achieve ideal results. This study explored the effect of Indian Hedgehog (IHH) on thin endometrium and its mechanism of action. A thin endometrial rat model was established by infusion of 95% ethanol. IHH was overexpressed in model rats using adeno-associated viruses. The endometrial thickness and number of glands and vessels were determined using H&E staining. Endometrial fibrosis was detected using Masson's trichrome staining. Immunohistochemistry was performed to detect α-SMA, MUC-1, and CK19. After modeling, the rats were mated, and the number of gestational sacs was counted for fertility assessment. Western blotting was used to detect the angiogenesis markers vWF, PCNA, and vim and Hedgehog signaling-related proteins SMO, GLI1, and GLI3. IHH overexpression reduced ethanol-induced edema and bruising, repaired the appearance of damaged tissue, increased endometrial thickness, promoted glandular and vascular regeneration, and alleviated endometrial fibrosis. IHH overexpression inhibited the expression of fibroblast marker α-SMA while promoting the expression of vWF, PCNA, vim, CK19, and MUC-1. It also increased the number of gestational sacs and promoted the expression of SMO, GLI1, and GLI3. In conclusion, IHH ameliorates ethanol-induced thin endometrium and improves fertility by activating the Hedgehog signaling pathway.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mice lacking two testis-specific cytoplasmic poly(A)-binding proteins, PABPC2 and PABPC6, exhibit normal spermatogenesis and fertility. 缺乏两种睾丸特异性细胞质聚(A)结合蛋白PABPC2和PABPC6的小鼠表现出正常的精子发生和生育能力。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-04-18 DOI: 10.1262/jrd.2025-012

Yuka Isono, Yuko Kaku, Yoshinori Kanemori, Shin-Ichi Kashiwabara

{"title":"Mice lacking two testis-specific cytoplasmic poly(A)-binding proteins, PABPC2 and PABPC6, exhibit normal spermatogenesis and fertility.","authors":"Yuka Isono, Yuko Kaku, Yoshinori Kanemori, Shin-Ichi Kashiwabara","doi":"10.1262/jrd.2025-012","DOIUrl":"https://doi.org/10.1262/jrd.2025-012","url":null,"abstract":"The cytoplasmic poly(A)-binding protein (PABPC) plays a central role in the life of poly(A) mRNAs, including their stability, translation, and decay. In addition to the nearly ubiquitous PABPC1, two testis-specific PABPCs, PABPC2 and PABPC6, are present in rodents, while one specific PABPC, PABPC3, is found in primate testes. These three PABPC proteins are each encoded by intronless genes that may have diverged independently due to the retroposition of prototypical Pabpc1 or PABPC1. PABPC2 and PABPC6 are distinguished from PABPC1 in that they barely associate with translationally active polysomal mRNAs and are enriched in male germ cell-specific nuage, termed chromatoid bodies. Despite these unique characteristics, spermatogenesis and male fertility were not compromised in mutant mice lacking either PABPC2 or PABPC6, suggesting functional redundancy between the two proteins. Here, we produced double-mutant mice lacking both PABPC2 and PABPC6 and found that the simultaneous absence of these two proteins failed to affect testicular protein synthesis, spermatogenesis, or male fertility in vivo. These results suggest that the functions of PABPC2 and PABPC6 are redundant with those of other coexisting PABPC proteins, including PABPC1. We propose that testis-specific PABPC proteins emerged because of transcriptional promiscuity in the testis.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can thermoregulatory response to heat stress be improved in lactating dairy cows? Insights from counter-current heat transfer systems impacting reproduction. 泌乳奶牛对热应激的体温调节反应能否得到改善？从影响生殖的逆流传热系统的见解。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-04-14 Epub Date: 2025-01-16 DOI: 10.1262/jrd.2024-101

Fernando López-Gatius

引用次数: 0

Factors influencing in vivo embryo production in Japanese Black donors: The role of anti-Müllerian hormone and inflammation parameters. 影响日本黑胚供体体内胚胎产生的因素：抗<s:1>勒氏杆菌激素和炎症参数的作用。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-04-14 Epub Date: 2025-01-31 DOI: 10.1262/jrd.2024-092

Hiroaki Okawa, Norihiro Yukiyama, Osamu Yamato, Akira Goto, Oky Setyo Widodo, Yasuo Fushimi, Mitsuhiro Takagi

{"title":"Factors influencing in vivo embryo production in Japanese Black donors: The role of anti-Müllerian hormone and inflammation parameters.","authors":"Hiroaki Okawa, Norihiro Yukiyama, Osamu Yamato, Akira Goto, Oky Setyo Widodo, Yasuo Fushimi, Mitsuhiro Takagi","doi":"10.1262/jrd.2024-092","DOIUrl":"10.1262/jrd.2024-092","url":null,"abstract":"In Japanese Black (JB) cattle, the number of transferable embryos produced after superovulation is crucial for the economic success of embryo production for both farmers and veterinarians. Anti-Müllerian hormone (AMH) has emerged as a key reproductive marker for predicting the number of embryos produced in vivo and oocytes retrieved through transvaginal pickup. This study investigated the relationship between AMH, inflammatory markers, including serum amyloid A (SAA) and albumin/globulin (A/G) ratio, and the number of embryos recovered and transferable after superovulation in JB cows. A total of 96 JB donor cows underwent artificial insemination after superovulation, and embryo recovery was performed 7 days later. Embryos recovered were classified based on the International Embryo Technology Society criteria, wherein \"transferable embryos\" included those with codes 1 or 2, while \"total embryos\" included transferable embryos as well as those with codes 3 and 4. Blood samples collected during embryo recovery were used to measure serum AMH, SAA, and A/G ratios. When grouped by AMH quartiles, the high-AMH and middle-high-AMH groups produced significantly more total embryos compared to the low-AMH group. The total number of embryos increased with higher AMH levels (r = 0.3336, P = 0.0009). Correlation analysis revealed associations between AMH, α1-globulin and SAA. Additionally, a significant positive correlation was observed between total and transferable embryos (r = 0.6339, P < 0.0001) and between AMH and the yield ratio (r = 0.25583, P = 0.0119). These findings confirm that AMH concentration is a valuable reproductive marker for predicting the total and transferable embryos produced by JB donor cows.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"93-98"},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11999828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influences of 5-hydroxytriptamine on sperm hyperactivation and in vitro fertility in rats. 5-羟曲坦胺对大鼠精子过度活化及体外生育能力的影响。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-04-14 Epub Date: 2025-01-25 DOI: 10.1262/jrd.2024-078

Yuki Koyano, Masakatsu Fujinoki

引用次数: 0

Involvement of nuclear receptor corepressor 2 (NCOR2) in estrogen-induced repression of arcuate Kiss1 expression in female rats. 核受体辅助抑制因子2 （NCOR2）参与雌激素诱导的雌性大鼠弓形吻素1表达抑制。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-04-14 Epub Date: 2025-01-27 DOI: 10.1262/jrd.2024-100

Marina Takizawa, Sae Miyazaki, Hitomi Tsuchida, Mayuko Nagae, Shunsuke Seki, Masumi Hirabayashi, Fumitaka Osakada, Naoko Inoue, Hiroko Tsukamura, Yoshihisa Uenoyama

{"title":"Involvement of nuclear receptor corepressor 2 (NCOR2) in estrogen-induced repression of arcuate Kiss1 expression in female rats.","authors":"Marina Takizawa, Sae Miyazaki, Hitomi Tsuchida, Mayuko Nagae, Shunsuke Seki, Masumi Hirabayashi, Fumitaka Osakada, Naoko Inoue, Hiroko Tsukamura, Yoshihisa Uenoyama","doi":"10.1262/jrd.2024-100","DOIUrl":"10.1262/jrd.2024-100","url":null,"abstract":"Hypothalamic arcuate (ARC) kisspeptin neurons are considered the gonadotropin-releasing hormone pulse generator in rats. In virgin rats, the expression of the ARC kisspeptin gene (Kiss1) is repressed by proestrous levels of estradiol-17β (high E2) but not by diestrous levels of E2 (low E2). In lactating rats, ARC Kiss1 expression is repressed by low E2 during late lactation. This study aimed to investigate whether nuclear receptor corepressor 2 (NCOR2, encoded by Ncor2), an estrogen receptor α corepressor, is involved in the estrogen-induced repression of ARC Kiss1 expression in rats. Double in situ hybridization for Kiss1 and Ncor2 revealed that approximately 80% of ARC Kiss1-expressing cells co-expressed Ncor2 in ovariectomized (OVX) + low E2 virgin rats, while approximately 90% of ARC Kiss1-expressing cells co-expressed Ncor2 in OVX + low E2 lactating rats. To further examine the role of Ncor2, we studied the effects of Kiss1-dependent Ncor2 knockdown on ARC Kiss1 expression and luteinizing hormone (LH) pulses. An adeno-associated virus vector carrying Cre-activated short hairpin RNA (shRNA) for Ncor2 was administered to the ARC in two Kiss1-Cre rat models: OVX + high E2 Kiss1-Cre virgin rats and OVX + low E2 Kiss1-Cre lactating rats. Ncor2-shRNA treatment significantly increased the number of ARC Kiss1-expressing cells and the intensity of Kiss1 signals in OVX + high E2 virgin rats but failed to fully restore low E2-induced Kiss1 repression in lactating rats. The Ncor2-shRNA treatment failed to affect LH pulses in both models. These findings suggest that NCOR2 in ARC kisspeptin neurons mediates high E2-induced repression of ARC Kiss1 expression in virgin rats.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"71-84"},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11999826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0