Journal of Reproduction and Development最新文献

The mechanism of heat stress-induced injury in the immature oocytes of zebrafish (Danio rerio). 热应激诱导斑马鱼未成熟卵母细胞损伤的机制。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-09-08 DOI: 10.1262/jrd.2025-050

Yanuar Achadri, Nao Kuwahara, Momoko Kimura, Nami Kanamaru, Kazutsugu Matsukawa, Keisuke Edashige

{"title":"The mechanism of heat stress-induced injury in the immature oocytes of zebrafish (Danio rerio).","authors":"Yanuar Achadri, Nao Kuwahara, Momoko Kimura, Nami Kanamaru, Kazutsugu Matsukawa, Keisuke Edashige","doi":"10.1262/jrd.2025-050","DOIUrl":"https://doi.org/10.1262/jrd.2025-050","url":null,"abstract":"Immature zebrafish oocytes are highly susceptible to high temperatures, making it difficult to warm cryopreserved oocytes rapidly. In the present study, we aimed to investigate whether thermosensitive channels, lipid mediators, and ferroptosis are involved in heat stress-induced injury in immature zebrafish oocytes. Oocytes were injected with inhibitors of a heat-sensitive channel (TRPV1) and multiple enzymes-cytosolic phospholipase A2α (cPLA2α), cyclooxygenases (COXs), arachidonate lipoxygenase 5 (ALOX5), and lysophosphatidylcholine acyltransferase 2 (LPCAT2). In addition, a ferroptosis-specific inhibitor was administered. The oocytes were then warmed at 45°C for 15 min, incubated at 25°C for 2 h, and then stained with propidium iodide. When the control oocytes were warmed at 45°C for 15 min, their survival was low (1%-8%). However, the survival of oocytes injected with the TRPV1-specific inhibitor markedly improved (40%), suggesting that TRPV1 activation triggers heat stress injury in oocytes. When a cPLA2α-specific inhibitor was injected, survival of oocytes after warming significantly improved (30%), suggesting that lipid mediators or ferroptosis are involved in heat stress-induced injury in oocytes. In contrast, survival either slightly improved or did not improve when oocytes were injected with specific inhibitors of COXs, ALOX5, and LPCAT2 (16%, 8%, and 3%, respectively). Notably, the ferroptosis-specific inhibitor markedly improved oocyte survival (60%). These results may facilitate methodological advancements in fish oocyte cryopreservation. Additionally, they suggest that ferroptosis is involved in heat stress-induced injury in immature zebrafish oocytes, following TRPV1 activation and subsequent cPLA2α activation.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of light wavelength on oocyte maturation, parthenogenesis, and parthenogenetic embryo development in pigs. 光波长对猪卵母细胞成熟、孤雌生殖和孤雌胚胎发育的影响。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-08-31 DOI: 10.1262/jrd.2025-028

Su Bin Jung, So Yeon Nam, Ha Rin Namkung, Ji Won Han, Seon Ah Ryu, Eun Song Lee, Seung Tae Lee

{"title":"Effects of light wavelength on oocyte maturation, parthenogenesis, and parthenogenetic embryo development in pigs.","authors":"Su Bin Jung, So Yeon Nam, Ha Rin Namkung, Ji Won Han, Seon Ah Ryu, Eun Song Lee, Seung Tae Lee","doi":"10.1262/jrd.2025-028","DOIUrl":"https://doi.org/10.1262/jrd.2025-028","url":null,"abstract":"Assisted reproductive technologies (ART) to generate developmentally competent oocytes necessitates light exposure due to the use of microscopes. Previous studies in several species have reported that the wavelength of light during the light exposure period is a critical factor in embryo development. However, the effects of different light wavelengths on embryo development in pigs remain unexplored. This study aimed to identify the optimal light conditions to enhance oocyte maturation, parthenogenetic activation of mature oocytes, and pre-implantation development of parthenogenetic embryos in pigs. Conducted irradiation experiments during in vitro maturation (IVM), parthenogenesis (PG), and in vitro culture (IVC) using visible (390-750 nm), blue (445-500 nm), green (500-575 nm), yellow (575-585 nm), and red (620-750 nm) light. Variation in light wavelengths during IVM, PG, or IVC did not significantly influence oocyte maturation of cumulus-oocyte complexes (COCs) derived from median antral follicles (MAFs), developmental competence of invitro-culture COCs after PG, and the production of blastocysts. However, continuous irradiation with green light throughout the entire process (IVM, PG, and IVC) significantly enhanced in vitro blastocyst production, and the resulting blastocysts showed significantly elevated HSP70 expression and a numerical increase in PCNA expression. We recommend conducting all in vitro procedures (IVM, PG, and IVC) for producing blastocysts from MAFs in porcine models under green light. This study will contribute to yielding higher success rates of porcine ART and reduce artificial stress to oocytes and embryos caused by in vitro manipulation under strong light exposure.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144958404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of luteal blood flow on endometrial progesterone concentrations and gene expression in Japanese Black cows. 黄体血流量对日本黑牛子宫内膜孕酮浓度及基因表达的影响。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-08-31 DOI: 10.1262/jrd.2025-032

Tomomi Kanazawa, Motohide Seki, Kosuke Iga, Keiichiro Kizaki

{"title":"Effects of luteal blood flow on endometrial progesterone concentrations and gene expression in Japanese Black cows.","authors":"Tomomi Kanazawa, Motohide Seki, Kosuke Iga, Keiichiro Kizaki","doi":"10.1262/jrd.2025-032","DOIUrl":"https://doi.org/10.1262/jrd.2025-032","url":null,"abstract":"Luteal blood flow (LBF) is essential for progesterone (P4) biosynthesis in the corpus luteum (CL) and affects bovine fertility. However, the mechanism by which LBF affects fertility remains unclear. This study was conducted to investigate the effects of LBF on endometrial P4 concentrations and gene expression. Endometrial biopsies and blood samples were collected from 13 Japanese Black cows after ultrasound examination on Day 7 (Day 0 = day of estrus). Based on LBF, the cows were divided into low- (LV; n = 5), medium- (MV; n = 2), and high-vascularity (HV; n = 6) groups. Plasma and endometrial P4 concentrations were measured using enzyme immunoassays. RNA sequencing was performed to compare the endometrial gene expression profiles from three cows in each of the LV and HV groups. Reverse transcription-quantitative PCR was performed for genes selected from the differentially expressed genes (DEGs), P4 receptors (PGR, PGRMC1, and PGRMC2), and P4-regulated genes (ANPEP, DGAT2, DKK1, and LTF). No differences were observed in plasma or endometrial P4 concentrations between the HV and LV groups. CCN3 was identified as a DEG between the HV and LV groups and was upregulated in the HV group. Compared to those of the LV group, the HV group exhibited higher CCN3 and PGR mRNA expression levels and lower ANPEP, DGAT2, and DKK1 mRNA expression levels. In conclusion, LBF affects endometrial gene expression without changing plasma or endometrial P4 concentrations on Day 7.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144958445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early pregnancy detection in ruminants: challenges and innovations. 反刍动物早期妊娠检测：挑战与创新。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-08-16 DOI: 10.1262/jrd.2025-048

Jakia Sultana, Sanjita Rani Paul, Md Sayaduzzaman Arafath, Md Hasanur Alam, Md Sharoare Hossain, Mohammad Moniruzzaman

{"title":"Early pregnancy detection in ruminants: challenges and innovations.","authors":"Jakia Sultana, Sanjita Rani Paul, Md Sayaduzzaman Arafath, Md Hasanur Alam, Md Sharoare Hossain, Mohammad Moniruzzaman","doi":"10.1262/jrd.2025-048","DOIUrl":"https://doi.org/10.1262/jrd.2025-048","url":null,"abstract":"Precise and early pregnancy detection is crucial for better breeding management and enhancing the overall production of ruminant livestock. Throughout the years, numerous methods have evolved for pregnancy detection in ruminants, each possessing specific advantages and limitations. This review thoroughly discusses both traditional and emergent diagnostic methods, emphasizing their principles, implementation, merits and challenges. Behavioral observation, rectal palpation and ultrasonography are the traditional approaches widely used because of their accessibility and direct detection of pregnancy conditions. Progesterone measurement, pregnancy-associated glycoprotein detection, and estrone sulfate examination are the hormonal assays that provide biochemical proof at specific phases of gestation. Recently, the analysis of interferon-stimulated gene expression and circulating microRNAs has shown promising roles in early pregnancy detection at the genetic and transcriptomic levels. The investigation of volatile organic compounds is a novel approach in pregnancy diagnosis, though it is non-invasive, and further confirmation is required for regular application. This review highlighted the importance of incorporating multiple examination strategies to enhance the accuracy and reliability of pregnancy detection in ruminants. Future research should center on the refinement and field application of advanced technologies to ensure their proper implementation in diverse ruminant production systems.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel method of correlative light and electron microscopy in cryosectioning of bovine anterior pituitary tissue using NanoSuit CLEM. 应用NanoSuit CLEM进行牛垂体前叶冷冻切片的相关光镜和电镜观察。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-08-14 DOI: 10.1262/jrd.2025-025

Hiroya Kadokawa, Hideya Kawasaki

{"title":"A novel method of correlative light and electron microscopy in cryosectioning of bovine anterior pituitary tissue using NanoSuit CLEM.","authors":"Hiroya Kadokawa, Hideya Kawasaki","doi":"10.1262/jrd.2025-025","DOIUrl":"https://doi.org/10.1262/jrd.2025-025","url":null,"abstract":"Correlative light-electron microscopy (CLEM) combines fluorescence microscopy and scanning electron microscopy (SEM) to achieve nanoscale resolution while highlighting regions of interest identified by fluorescence microscopy. CLEM is becoming increasingly important in life sciences but traditionally requires highly dried samples to withstand the high vacuum of SEM. The NanoSuit method, which mimics native extracellular substances, was developed to address this limitation by encasing samples in a thin, vacuum-proof membrane, allowing SEM observation of live or wet multicellular organisms. While previous NanoSuit CLEM studies focused on formalin-fixed paraffin-embedded sections and cultured cells, cryosections had not yet been explored. In this study, NanoSuit CLEM with diluted NanoSuit solution was applied to cryosections of bovine anterior pituitary tissue. Secretory granules in gonadotrophs, which constitute less than 12% of anterior pituitary cells, were successfully visualized. However, other organelles remained unobserved due to fixation conditions. Therefore, NanoSuit CLEM enabled visualization of the ultrastructure of important cells in cryosections, even from large animals.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144847147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simplified protocol for vitrification of hamster embryos. 仓鼠胚胎玻璃化的简化方法。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-08-04 DOI: 10.1262/jrd.2025-036

Michiko Hirose, Nami Morishita, Ayumi Hasegawa, Keiji Mochida, Toshiko Tomishima, Kimiko Inoue, Toshitaka Horiuchi, Atsuo Ogura

引用次数: 0

Tryptophan promotes sperm hyperactivation in hamsters via 5-hydroxytryptamine biosynthesis within sperm. 色氨酸通过在仓鼠精子内的5-羟色胺生物合成促进精子过度活化。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-08-03 DOI: 10.1262/jrd.2025-006

Issei Suzuki, Masakatsu Fujinoki, Takao Kamai

引用次数: 0

A prospective randomized trial comparing dephereline and busereline for ovulation induction in heat-stressed lactating dairy cows. 一项比较去phereline和busereline对热应激泌乳奶牛促排卵作用的前瞻性随机试验。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-08-01 Epub Date: 2025-06-12 DOI: 10.1262/jrd.2025-023

Fernando López-Gatius

引用次数: 0

Reduced HSP70 expression under mild hypothermia is involved in bovine embryo growth suppression. 亚低温条件下HSP70表达的降低与牛胚胎生长抑制有关。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-08-01 Epub Date: 2025-05-11 DOI: 10.1262/jrd.2024-087

Toshimichi Ishii, Sho Nakamura, Satoshi Ohkura, Shuichi Matsuyama

{"title":"Reduced HSP70 expression under mild hypothermia is involved in bovine embryo growth suppression.","authors":"Toshimichi Ishii, Sho Nakamura, Satoshi Ohkura, Shuichi Matsuyama","doi":"10.1262/jrd.2024-087","DOIUrl":"10.1262/jrd.2024-087","url":null,"abstract":"Bovine blastocysts cultured under mild hypothermia (MH) can be maintained with non-hatching viable embryos compared to normothermic controls (38.5°C). However, the mechanism by which mildly hypothermic culture delays embryonic growth has not yet been elucidated. This study evaluated the number of cells in embryos cultured under MH conditions and the expression of genes involved in embryonic differentiation. Bovine blastocysts cultured under MH conditions exhibited reduced cell numbers and interferon-tau mRNA expression. Both forkhead box O3 (FOXO3) mRNA expression and FOXO3 protein level in blastocysts cultured under MH conditions were higher than those in normothermic controls (P < 0.05). On the phosphorylated FOXO3 protein level, there was no significant difference between blastocysts cultured under MH and normothermic conditions. In contrast, no significant difference was observed in the ATP content of blastocysts between the MH and normothermic groups. In blastocysts cultured under MH conditions, cold-inducible RNA-binding protein (CIRP) and RNA-binding motif protein 3 (RBM3) mRNA expression increased, and heat shock protein 70 (HSP70) mRNA expression decreased compared to that in normothermic controls (P < 0.05). Considering that HSP70 is involved in preventing apoptosis, these results suggest that MH retards embryonic development via apoptosis induced by HSP70 downregulation during the culture period.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"195-200"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12322498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of chemosynthetic choline plasmalogens on gonadotropin secretion from bovine gonadotrophs. 化学合成胆碱缩醛原对牛促性腺激素分泌的影响。

IF 2.2 4区生物学

Journal of Reproduction and Development Pub Date : 2025-08-01 Epub Date: 2025-06-07 DOI: 10.1262/jrd.2025-019

Hiroya Kadokawa, Yvan Bienvenu Niyonzima, Takatsugu Hirokawa, Ryunosuke Yoshino

{"title":"Effects of chemosynthetic choline plasmalogens on gonadotropin secretion from bovine gonadotrophs.","authors":"Hiroya Kadokawa, Yvan Bienvenu Niyonzima, Takatsugu Hirokawa, Ryunosuke Yoshino","doi":"10.1262/jrd.2025-019","DOIUrl":"10.1262/jrd.2025-019","url":null,"abstract":"Ethanolamine plasmalogens (EPls) and choline plasmalogens (CPls), unique glycerophospholipids may play important roles in milk production and reproduction in postpartum dairy cows. While CPls are more abundant in bovine blood, EPls are predominant in the brain. Brain EPls are the only recognized ligands of G protein-coupled receptor 61 (GPR61), a receptor that co-localizes with GnRH receptors on gonadotrophs. We hypothesized that chemosynthetic CPls stimulate gonadotropin secretion from bovine gonadotrophs, similar to the reported effects of chemosynthetic EPls. Anterior pituitary cells from healthy, post-pubertal heifers, were cultured for 3.5 days and then treated with increasing concentrations (0, 0.7, 7, 70, or 700 pM) of EPl with vinyl-ether-bonded stearic acid and ester-bonded oleic acid (C18:0-C18:1EPl) as a positive control, or CPls with vinyl-ether-bonded stearic acid and ester-bonded oleic acid (C18:0-C18:1CPl), arachidonic acid (C18:0-C20:4CPl), or docosahexaenoic acid (C18:0-C22:6CPl). After 2 h, the medium samples were harvested for FSH and LH assays. C18:0-C18:1EPl (7-700 pM) stimulated basal FSH and LH secretion (P < 0.01). None of the tested CPl concentrations stimulated LH secretion. Only 700 pM of C18:0-C18:1CPl, but not lower concentrations, stimulated FSH secretion (P < 0.05), an effect that was inhibited by a SMAD pathway inhibitor. However, both C18:0-C18:1CPl and C18:0-C20:4CPl synergized with GnRH to stimulate FSH secretion. In silico molecular-docking simulations using the deep-learning algorithm ColabFold revealed that CPls bind to the three-dimensional structural model of GPR61. In conclusion, C18:0-C20:4CPl stimulated FSH secretion exclusively in the presence of GnRH, whereas C18:0-C18:1CPl weakly stimulated FSH secretion and showed potential interaction with the GnRH signaling pathways.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"201-209"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12322497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0