Journal of Reproduction and Development最新文献

Ccdc152 is not necessary for male fertility, but contributes to maintaining sperm morphology. Ccdc152 不是男性生育所必需的，但有助于维持精子的形态。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-26 DOI: 10.1262/jrd.2024-058

Ryua Harima, Takahiro Sasaki, Takayuki Kaneko, Fuka Aso, Hayato Takashima, Takashi Toyama, Kenshiro Hara, Kentaro Tanemura, Yoshiro Saito

{"title":"Ccdc152 is not necessary for male fertility, but contributes to maintaining sperm morphology.","authors":"Ryua Harima, Takahiro Sasaki, Takayuki Kaneko, Fuka Aso, Hayato Takashima, Takashi Toyama, Kenshiro Hara, Kentaro Tanemura, Yoshiro Saito","doi":"10.1262/jrd.2024-058","DOIUrl":"https://doi.org/10.1262/jrd.2024-058","url":null,"abstract":"Selenoprotein P (SeP) is synthesized in the liver and plays a vital role in maintaining selenium homeostasis via transport throughout the body. Previous studies have shown that SeP-deficient mice have severely reduced expression of selenoproteins essential for testicular function, leading to male infertility. We previously reported that the high expression of Ccdc152 in hepatocytes acts as a lncRNA, suppressing SeP expression in the liver. Ccdc152 reduces SeP translation by binding to SeP mRNA and decreasing its interaction with SECIS-binding protein 2. Although Ccdc152 is highly expressed in testes, its function remains unclear. Therefore, this study aimed to elucidate the role of Ccdc152 in the testes. Using the CRISPR/Cas9 system, we generated mice lacking all exons of Ccdc152 and found that SeP expression levels in the liver and plasma, as well as overall selenium homeostasis, remained unchanged. No significant differences were observed in the expression of glutathione peroxidase 1/4 or level of selenium in the testes. Subsequent investigation of the impact on male reproductive function revealed no abnormalities in sperm motility or Mendelian ratios of the offspring. However, a slight decrease in testicular weight and an increased rate of sperm malformations in the epididymis were observed. RNA-seq and pathway analyses identified the reduced expression of multiple genes related to kinesin and reproductive pathways. Based on these findings, Ccdc152 may not be essential for male reproductive function, but it may enhance reproductive capabilities by maintaining the expression of genes necessary for reproduction.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fertilization and developmental competence of in vitro fertilized embryos from C57BL/6J mice of different ages and the impact of vitrification. 不同年龄 C57BL/6J 小鼠体外受精胚胎的受精和发育能力以及玻璃化的影响。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-25 DOI: 10.1262/jrd.2024-082

Seiji Kito

{"title":"Fertilization and developmental competence of in vitro fertilized embryos from C57BL/6J mice of different ages and the impact of vitrification.","authors":"Seiji Kito","doi":"10.1262/jrd.2024-082","DOIUrl":"https://doi.org/10.1262/jrd.2024-082","url":null,"abstract":"Prepubertal animals are often preferred as sources of oocytes for assisted reproductive technologies (ARTs) in laboratory mice, but the normality and developmental competence of these oocytes remain controversial. This study systematically examined in vitro fertilization competence, embryo development, and fetal development after embryo transfer (ET) using oocytes from C57BL/6J mice aged 3 to 10 weeks. Oocytes were collected from superovulated females, fertilized, and cultured in vitro for 96 h or transferred at 2-cell stage to recipient females. Additionally, fetal development was compared between unfrozen and frozen-thawed in vitro-fertilized 2-cell embryos across different age groups. The number of ovulated oocytes per animal decreased with age, while the percentage of morphologically normal oocytes was highest in 3-week-old mice (99%) compared to older ages (70-86%, P < 0.05). Although fertilization percentages were consistently high (≥ 97%), blastocyst development in vitro, the nuclear counts of blastocysts and fetal development after ET were lowest for embryos from 3-week-old mice. Development of frozen-thawed embryos to fetuses was significantly reduced compared to unfrozen embryos in all age groups, except for those from 10-week-old mice. These findings suggest that oocytes from prepubertal mice, particularly from 3-week-old mice, are less developmentally competent than those from older mice. Therefore, the age of animals for oocyte source should be carefully considered based on the specific requirements of the research or ART applications.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AVPV Kiss1 neuron-specific knockdown of purinergic P2X2 receptor suppresses LH surge and ovulation in Kiss1-Cre rats. AVPV Kiss1神经元特异性敲除嘌呤能P2X2受体可抑制Kiss1-Cre大鼠的LH激增和排卵。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-20 DOI: 10.1262/jrd.2024-046

Safiullah Hazim, Shunsuke Seki, Ryoya Yabushita, Mayuko Nagae, Hitomi Tsuchida, Masumi Hirabayashi, Yoshihisa Uenoyama, Hiroko Tsukamura, Naoko Inoue

{"title":"AVPV Kiss1 neuron-specific knockdown of purinergic P2X2 receptor suppresses LH surge and ovulation in Kiss1-Cre rats.","authors":"Safiullah Hazim, Shunsuke Seki, Ryoya Yabushita, Mayuko Nagae, Hitomi Tsuchida, Masumi Hirabayashi, Yoshihisa Uenoyama, Hiroko Tsukamura, Naoko Inoue","doi":"10.1262/jrd.2024-046","DOIUrl":"https://doi.org/10.1262/jrd.2024-046","url":null,"abstract":"Ovulation disorders are a major cause of low pregnancy rates and infertility in humans and livestock. Kisspeptin neurons located in the anteroventral periventricular nucleus (AVPV) are responsible for the generation of the gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) surge and the consequent ovulation in female rodents. The present study aimed to examine whether purinergic neurons are direct upstream stimulators of AVPV kisspeptin neurons that trigger the GnRH/LH surge and consequent ovulation in Kiss1-Cre rats. We specifically knocked down the mRNA expression of the P2rx2 purinergic receptor in AVPV kisspeptin neurons by administering an adeno-associated virus (AAV) vector containing Cre-dependent P2rx2 short hairpin RNA (shRNA) into the AVPV region of ovariectomized (OVX) Kiss1-Cre rats treated with a proestrus level of estradiol-17β (OVX + high E2) or ovary-intact Kiss1-Cre rats. The E2-induced afternoon LH surge was significantly suppressed by AVPV kisspeptin neuron-specific knockdown of P2rx2 in OVX + high E2 Kiss1-Cre rats compared with scrambled shRNA-treated control OVX + high E2 Kiss1-Cre rats. Furthermore, the specific knockdown of P2rx2 in AVPV kisspeptin neurons largely disrupted the estrous cycle, spontaneous LH surge, and ovulation in ovary-intact Kiss1-Cre rats. These findings suggest that purinergic neurons directly stimulate AVPV kisspeptin neurons via P2X2 receptors (P2RX2) to induce the GnRH/LH surge and consequent ovulation in female rats.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of anti-Müllerian hormone levels as a reproductive indicator in Japanese Black cattle. 评估作为日本黑牛繁殖指标的抗缪勒氏管激素水平。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-17 DOI: 10.1262/jrd.2024-047

Takeshi Koyama, Hiromi Suzuki, Miki Shimizu, Riuru Mizuno, Ayaha Ishigami, Nobuyuki Kamidate, Yoshihisa Otani, Michiko Okubo, Kousaku Souma, Hiroki Hirayama

{"title":"Assessment of anti-Müllerian hormone levels as a reproductive indicator in Japanese Black cattle.","authors":"Takeshi Koyama, Hiromi Suzuki, Miki Shimizu, Riuru Mizuno, Ayaha Ishigami, Nobuyuki Kamidate, Yoshihisa Otani, Michiko Okubo, Kousaku Souma, Hiroki Hirayama","doi":"10.1262/jrd.2024-047","DOIUrl":"https://doi.org/10.1262/jrd.2024-047","url":null,"abstract":"The size of the ovarian reserve, an indicator of the number of primordial follicles, varies widely among individuals, and declines with age. However, the association between the ovarian reserve and fertility remains unclear. Therefore, in the present study, we analyzed the relationship between plasma concentrations of anti-Müllerian hormone (AMH), a marker of ovarian reserve, and reproductive outcomes in Japanese Black cattle. AMH level quartiles were positively associated with pregnancy following artificial insemination (AI), and the median number of days to pregnancy in Q4 (13 days, 95% confidence interval [CI] = 7-18 days) was significantly shorter than that in Q1 (21 days, 95% CI = 15-46 days). The odds ratio for the predicted pregnancy rate by logistic regression analysis in Q4 (4.06, 95% CI = 1.54-10.67) was also significantly higher than that in Q1. Plasma AMH concentrations were significantly higher in summer (June-August) than in winter (December-February). Furthermore, a strong correlation (r = 0.829, P < 0.001) was observed between plasma AMH concentrations at 2 and 14 months of age. Calves with plasma AMH concentrations of > 700 pg/ml at 2 months old showed a transient increase and maximum AMH concentration within 5 months of birth. Overall, the results of this study indicate that the plasma AMH concentration serves as a predictive marker for the probability of conception following AI in Japanese Black cattle. The current findings contribute to the reliable assessment of AMH production and the early prediction of reproductive performance in sexually mature heifers.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Change in the ability of bovine granulosa cells to elongate transzonal projections and their transcriptome changes during follicle development. 卵泡发育过程中牛颗粒细胞伸长横区突起的能力变化及其转录组变化。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-14 DOI: 10.1262/jrd.2024-016

Mihoko Fushii, Hirohisa Kyogoku, Jibak Lee, Takashi Miyano

{"title":"Change in the ability of bovine granulosa cells to elongate transzonal projections and their transcriptome changes during follicle development.","authors":"Mihoko Fushii, Hirohisa Kyogoku, Jibak Lee, Takashi Miyano","doi":"10.1262/jrd.2024-016","DOIUrl":"https://doi.org/10.1262/jrd.2024-016","url":null,"abstract":"Granulosa cells (GCs) in secondary follicles differentiate into cumulus cells (CCs) and mural granulosa cells (MGCs) in the antral follicle. Only CCs maintain direct connections with oocytes through transzonal projections (TZPs) and support oocyte growth. Here, we examined whether granulosa cells (GCs) from secondary follicles and MGCs from early and late antral follicles were able to reconstruct complexes with TZP-free denuded oocytes (DOs) and regenerate TZPs. Furthermore, to confirm that the regenerated TZPs were functional, the development of the reconstructed complexes and oocyte growth in the complexes were evaluated. After coculture, GCs and MGCs from early antral follicles reconstructed the complexes with DOs and regenerated TZPs. Furthermore, the oocytes in the integrally reconstructed complexes grew fully and acquired meiotic competence, suggesting that the regenerated TZPs were functional. In contrast, MGCs from the late antral follicles lost their ability to elongate TZPs. As the ability to regenerate TZPs differed among cells, we analyzed the transcriptomes of GCs, CCs, and MGCs collected from follicles of different sizes. The characteristics of TZP generation coincided with the transcriptome changes in two directions: from GCs to CCs and MGCs. In conclusion, until the early antral follicle stage, bovine GCs, CCs, and MGCs have common characteristics to elongate TZPs and form antrum-like structures that support oocyte growth in vitro. Furthermore, as the follicle develops, MGCs lose the ability to elongate TZPs.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagy in the corpus luteum correlates with tissue growth in pregnant rats. 黄体中的自噬与怀孕大鼠的组织生长有关。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-07-07 DOI: 10.1262/jrd.2024-019

Yasuaki Oishi, Koji Asakawa, Yuri Ishiwata, Shota Oka, Ryota Terashima, Makoto Sugiyama, Keiichiro Kizaki, Mitsumori Kawaminami, Shiro Kurusu

{"title":"Autophagy in the corpus luteum correlates with tissue growth in pregnant rats.","authors":"Yasuaki Oishi, Koji Asakawa, Yuri Ishiwata, Shota Oka, Ryota Terashima, Makoto Sugiyama, Keiichiro Kizaki, Mitsumori Kawaminami, Shiro Kurusu","doi":"10.1262/jrd.2024-019","DOIUrl":"10.1262/jrd.2024-019","url":null,"abstract":"The developmental activation of the corpus luteum (CL) structurally and functionally is critical for the temporally regulated establishment, maintenance, and termination of pregnancy in rats. In this study, we have investigated the possible involvement of autophagy in the regulation of the CL during pregnancy in rats. The expression ratio of microtubule-associated protein light chain 3 (LC3)-II/-I, a widely used indicator of autophagic activity, in the CL remained relatively stable until day 15 of pregnancy. Subsequently, it progressively increased until day 21, and then declined until day 3 postpartum. This fluctuation was closely associated with the tissue weight of the CL rather than progesterone (P4) production activity. Light and electron microscopy revealed the presence of immunoreactive LC3 aggregates and irregularly shaped autolysosome-like microstructures in the cytoplasm of luteal cells during late pregnancy. Notably, a bolus intrabursal injection of the autophagy inhibitor bafilomycin A1 on day 15 of pregnancy resulted in a significant reduction in luteal cell size and disrupted the normal alteration of circulating P4 levels. Consequently, treatment with this inhibitor increased the likelihood of the varied timing (both advanced and delayed) of delivery and led to reduced body weight in neonates when compared with the vehicle-treated control group. Our findings suggest that autophagy in the rat CL contributes to luteal tissue growth, influences P4 production, and thereby fine-tunes the regulation of gestation length in rats.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dairy cow parity affects relationships among nutritional parameters in the blood of dams, umbilical cords, and calves and placental development at calving. 奶牛奇数影响母牛、脐带和犊牛血液中的营养参数与产犊时胎盘发育之间的关系。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-07-07 DOI: 10.1262/jrd.2024-010

Riku Mashimo, Hanon Ohban, Yuka Kumazaki, Sayaka Ito, Tomono Katagiri, Nobuyuki Kusaba, Chiho Kawashima

{"title":"Dairy cow parity affects relationships among nutritional parameters in the blood of dams, umbilical cords, and calves and placental development at calving.","authors":"Riku Mashimo, Hanon Ohban, Yuka Kumazaki, Sayaka Ito, Tomono Katagiri, Nobuyuki Kusaba, Chiho Kawashima","doi":"10.1262/jrd.2024-010","DOIUrl":"10.1262/jrd.2024-010","url":null,"abstract":"Heifer growth and milk production in lactating cows may diminish the nutrient supply to the fetus. This study aimed to analyze the characteristics of the nutrient supply to the fetus in primiparous and multiparous cows. We investigated maternal, umbilical cord, and calf blood glucose and amino acid levels, as well as placental development in 28 primiparous (PP) and 30 multiparous (MP) Holstein cows. Although the total cotyledonary weight and surface area showed no significant differences, the MP group exhibited larger individual cotyledons (P < 0.01) and fewer medium-sized cotyledons (P < 0.05). Within the PP group, total cotyledonary weight and surface area positively correlated with blood glucose (r = 0.71-0.77; P < 0.01) and total essential amino acid (r = 0.55; P < 0.05) concentrations in the umbilical veins. However, no significant correlation was observed in the MP group. Blood glucose and amino acid concentrations in the umbilical vein, umbilical artery, and calf were significantly lower in the MP group (P < 0.05), although no difference was observed in the dams between the groups. In conclusion, the nutrient status of primiparous cows can alter fetal nutrient supply. Moreover, multiparous cows have larger individual cotyledons as an adaptive response to increased milk production during pregnancy. However, this adaptive response in multiparous cows did not completely restore nutrient supply to the fetus to the same extent as that in primiparous cows. Therefore, the nutritional management of multiparous cows during pregnancy must be reconsidered.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cpne1 deficiency preserves sperm motility under Ca²⁺ channel blockade. 在 Ca2+ 通道阻断的情况下，Cpne1 的缺乏可保持精子活力。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-07-14 DOI: 10.1262/jrd.2024-027

Qiang Xie, Hanbin Zhang, Yuge Zhuang, Jinsheng Liu, Zicong Huang, Xiaoyuan Zhang, Ke Ma, Wenyuan Liu, Minyu Xie, Chuyu Huang, Xiaojing Zhong, Feilong Chen, Feng Zou, Wansong Zhang, Chunming Qiu, Canbiao Sun, Xiangjin Kang, Zhenguo Chen, Guofei Zhang

{"title":"Cpne1 deficiency preserves sperm motility under Ca2+ channel blockade.","authors":"Qiang Xie, Hanbin Zhang, Yuge Zhuang, Jinsheng Liu, Zicong Huang, Xiaoyuan Zhang, Ke Ma, Wenyuan Liu, Minyu Xie, Chuyu Huang, Xiaojing Zhong, Feilong Chen, Feng Zou, Wansong Zhang, Chunming Qiu, Canbiao Sun, Xiangjin Kang, Zhenguo Chen, Guofei Zhang","doi":"10.1262/jrd.2024-027","DOIUrl":"10.1262/jrd.2024-027","url":null,"abstract":"Calcium ions (Ca2+) play crucial roles in sperm motility and fertilization. The copine (CPNE) family comprises several Ca2+-dependent phospholipid-binding proteins. Of these, CPNE1 is extensively expressed in mammalian tissues; however, its precise role in testicular development and spermatogenesis is yet to be fully characterized. In this study, we used proteomics to analyze testicular biopsies and found that levels of CPNE1 were significantly reduced in patients with non-obstructive azoospermia (defective spermatogenesis) compared to those in patients with obstructive azoospermia (physiological spermatogenesis). In mice, CPNE1 is expressed at various stages of germ cell development and is associated with the Golgi apparatus. Ultimately, CPNE1 is expressed in the flagella of mature sperms. To further examine the role of CPNE1, we developed a Cpne1 knockout mouse model. Analysis showed that the loss of Cpne1 did not impair testicular development, spermatogenesis, or sperm morphology and motility in physiological conditions. When treated with gadolinium (III) chloride or 2-aminoethoxydiphenyl borate, known inhibitors of store-operated Ca2+ entry, Ca2+ signals and sperm motility were significantly compromised in wild-type mice; however, both mechanisms were conserved in KO mice. These results suggested that CPNE1 is dispensable for testicular development, spermatogenesis or sperm motility in physiological conditions. In addition, CPNE1 may represent a target of Ca2+ channel inhibitors and may therefore be implicated in the regulation of Ca2+ signaling and sperm motility.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perilipin2 depletion causes lipid droplet enlargement in the ovarian corpus luteum in mice. 消耗 Perilipin2 会导致小鼠卵巢黄体脂滴增大。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-07-15 DOI: 10.1262/jrd.2024-023

Megumi Ibayashi, Takayuki Tatsumi, Satoshi Tsukamoto

{"title":"Perilipin2 depletion causes lipid droplet enlargement in the ovarian corpus luteum in mice.","authors":"Megumi Ibayashi, Takayuki Tatsumi, Satoshi Tsukamoto","doi":"10.1262/jrd.2024-023","DOIUrl":"10.1262/jrd.2024-023","url":null,"abstract":"Lipid droplets (LDs) are endoplasmic reticulum-derived organelles that store neutral lipids (mostly triglycerides and cholesterol esters) within a phospholipid monolayer and appear in most eukaryotic cells. Perilipins (PLINs, comprising PLIN1-5) are abundant LD-associated proteins with highly variable expression levels among tissues. Although PLINs are expressed in the mammalian ovaries, little is known about their subcellular localization and physiological functions. In this study, we investigated the localization of PLIN1-3 and their relationship with LD synthesis using mCherry-HPos reporter mice, thereby enabling the visualization of LD biogenesis in vivo. PLIN2 and PLIN3 were localized as puncta in granulosa cells with low levels of LD synthesis in developing follicles. This localization pattern was quite different from that of PLIN1, which was mainly localized in the theca and interstitial cells with high levels of LD synthesis. In the corpus luteum, where LD synthesis is highly induced, PLIN2 and PLIN3 are abundant in the particulate structures, whereas PLIN1 is poorly distributed. We also generated global Plin2-deficient mice using the CRSPR/Cas9 system and demonstrated that the lack of PLIN2 did not alter the distribution of PLIN1 and PLIN3 but unexpectedly induced LD enlargement in the corpus luteum. Collectively, our results suggest that the localization of PLIN1-3 is spatiotemporally regulated and that PLIN2 deficiency influences LD mobilization in the corpus luteum within the ovaries.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Central δ/κ opioid receptor signaling pathways mediate chronic and/or acute suckling-induced LH suppression in rats during late lactation. 中枢δ/κ阿片受体信号通路介导哺乳后期大鼠慢性和/或急性吸吮引起的LH抑制。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-08-16 DOI: 10.1262/jrd.2024-045

Yoshihisa Uenoyama, Miku Nonogaki, Hitomi Tsuchida, Marina Takizawa, Sena Matsuzaki, Naoko Inoue, Hiroko Tsukamura

{"title":"Central δ/κ opioid receptor signaling pathways mediate chronic and/or acute suckling-induced LH suppression in rats during late lactation.","authors":"Yoshihisa Uenoyama, Miku Nonogaki, Hitomi Tsuchida, Marina Takizawa, Sena Matsuzaki, Naoko Inoue, Hiroko Tsukamura","doi":"10.1262/jrd.2024-045","DOIUrl":"10.1262/jrd.2024-045","url":null,"abstract":"In mammals, secretion of tonic (pulsatile) gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) is often suppressed during lactation. Suppression of GnRH/LH pulses in lactating dams is assumed to be caused by suckling stimuli and a chronic negative energy balance due to milk production. The present study aimed to investigate whether the central enkephalin-δ opioid receptor (DOR) signaling mediated the suppression of LH secretion by acute suckling stimuli and/or chronic negative energy balance due to milk production in rats during late lactation when dams were under a heavy energy demand. On postpartum day 16, the number of Penk (enkephalin mRNA)-expressing cells in the arcuate nucleus was significantly higher in lactating rats than in non-lactating control rats. Pulsatile LH secretion was suppressed in rats with chronic suckling or acute 1-h suckling stimuli 6 h after pup removal on day 16 of lactation. Central DOR antagonism significantly increased the mean LH concentrations and the baseline of LH pulses in rats with chronic suckling but not with acute suckling stimuli on day 16 of lactation. Besides, central κ opioid receptor (KOR) antagonism increased the amplitude of LH pulses in rats with the acute suckling stimuli on day 16 of lactation. These results suggest that central DOR signaling mediates the suppression of LH secretion caused by a negative energy balance in rats receiving chronic suckling during late lactation. On the other hand, central KOR signaling likely mediates acute suckling stimuli-induced suppression of LH secretion in rats during late lactation.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0