Journal of Reproduction and Development最新文献

Inexpensive thermal containers and insulation materials prevent deterioration of semen parameters for less than 90 minutes.

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-04-06 DOI: 10.1262/jrd.2025-001

Erina Takayama, Hiroki Takeuchi, Hideaki Yajima, Sayako Enomoto, Mito Sakamoto, Mikiko Nishioka, Ryota Tachibana, Tomoaki Ikeda, Eiji Kondo

{"title":"Inexpensive thermal containers and insulation materials prevent deterioration of semen parameters for less than 90 minutes.","authors":"Erina Takayama, Hiroki Takeuchi, Hideaki Yajima, Sayako Enomoto, Mito Sakamoto, Mikiko Nishioka, Ryota Tachibana, Tomoaki Ikeda, Eiji Kondo","doi":"10.1262/jrd.2025-001","DOIUrl":"https://doi.org/10.1262/jrd.2025-001","url":null,"abstract":"Male infertility contributes substantially to overall infertility, with temperature changes adversely affecting the sperm quality. During infertility treatments, the exposure of home-collected semen to extreme temperatures during transport deteriorates the semen parameters. This study investigates the effectiveness of thermal containers and insulation materials for preserving semen quality at low temperatures. Semen samples from 35 healthy male partners undergoing fertility treatments were analyzed. The samples were segregated in three groups and assessed: standard collection containers (Group A), thermal containers (Group B), and thermal containers with warming materials (Group C). Samples exposed to 4°C exhibited a notable decline in motility and forward motility over time, whereas groups B and C maintained these parameters better. Group C maintained the internal temperature at approximately 20°C for up to 90 min, reducing cold-induced deterioration. These findings demonstrate that cost-effective thermal retention methods can preserve semen quality during transport, and potentially improve the outcomes of intrauterine insemination and in vitro fertilization.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CpG site methylation regulates mouse Rec8 gene promoter activity.

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-04-04 DOI: 10.1262/jrd.2024-077

Mei Rong, Na Feng, Jinghuan Li, Wuyun Dalai

{"title":"CpG site methylation regulates mouse Rec8 gene promoter activity.","authors":"Mei Rong, Na Feng, Jinghuan Li, Wuyun Dalai","doi":"10.1262/jrd.2024-077","DOIUrl":"https://doi.org/10.1262/jrd.2024-077","url":null,"abstract":"The Rec8 gene is specifically expressed in fetal and adult gonads. Although the importance of REC8 in gametogenesis is widely acknowledged, the mechanisms underlying its germ cell-specific expression remain unclear. In this study, we utilized the mouse Rec8 gene sequence to construct a 2577 bp sequence, which included intron 1 (180 bp), exon 1 (118 bp), and an upstream 2279 bp region. The dual-luciferase assay results showed significant differences in promoter activity between -650 bp and -385 bp and between -89 bp and -35 bp. This indicated that the core promoter region of the Rec8 gene may exist within these regions. Bisulfite sequencing PCR results showed that CpGs 10-19 were largely unmethylated in the testes but hypermethylated in other tissues. Interestingly, correlation analysis between CpG methylation status and Rec8 mRNA expression levels showed that methylation of CpGs 10 to 19 was negatively correlated with Rec8 mRNA expression levels (Pearson's r = -0.991, P = 0.009). Furthermore, RNA-Seq data and bioinformatic analyses suggested that the specific expression of Rec8 may be linked to the presence of TATA-like sequences within its core promoter region. Overall, these findings indicate that Rec8 expression is regulated by the low methylation of CpG sites and the presence of TATA-like sequences in its core promoter.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cells.

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-03-21 DOI: 10.1262/jrd.2024-105

Miku Otsugu, Ayumi Mine, Kurumi Fujiwara, Ayako Ichimura, Keiji Yamamoto, Ryo Tachihara, Hideaki Tomura

{"title":"Low pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cells.","authors":"Miku Otsugu, Ayumi Mine, Kurumi Fujiwara, Ayako Ichimura, Keiji Yamamoto, Ryo Tachihara, Hideaki Tomura","doi":"10.1262/jrd.2024-105","DOIUrl":"https://doi.org/10.1262/jrd.2024-105","url":null,"abstract":"An association has been reported between a lower pH in the uterus and an increased rate of implantation. How low pH regulates endometrial function is unclear. This study investigated the effect of low pH on the expression of leukemia inhibitory factor (LIF), which is crucial for implantation, in a human endometrial carcinoma cell line, rat endometrial stromal cells, and porcine endometrial cells. LIF mRNA expression was quantified by real-time PCR and protein expression was assessed using western blot analysis. LIF mRNA and protein expression increased at low pH in human endometrial carcinoma cells. Increased LIF mRNA expression was also detected at low pH in rat endometrial stromal and porcine endometrial cells, suggesting that low intrauterine pH may create favorable conditions for implantation and endometrial receptivity across species. The increase in LIF mRNA expression in the three cell types was attenuated by the addition of amiloride, indicating that low pH promotes the expression of LIF via amiloride-sensitive molecules in the endometrium.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving porcine in vitro blastocyst development using fetal bovine serum, amino acids, and insulin-transferrin-selenium.

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-03-08 DOI: 10.1262/jrd.2024-095

Le Ba Anh My, Nguyen Lien Boi Linh, Lam Chi Thien, Nhat-Thinh Nguyen, Nguyen Ngoc Thao Vy, Nguyen Van Thuan, Hong-Thuy Bui

{"title":"Improving porcine in vitro blastocyst development using fetal bovine serum, amino acids, and insulin-transferrin-selenium.","authors":"Le Ba Anh My, Nguyen Lien Boi Linh, Lam Chi Thien, Nhat-Thinh Nguyen, Nguyen Ngoc Thao Vy, Nguyen Van Thuan, Hong-Thuy Bui","doi":"10.1262/jrd.2024-095","DOIUrl":"https://doi.org/10.1262/jrd.2024-095","url":null,"abstract":"In vitro production of porcine-hatched blastocysts is important for various applications. However, the mechanobiology of blastocoel expansion and hatching remains poorly understood. Our study aimed to efficiently produce hatched blastocysts and investigate the hatching mechanics of late-stage porcine embryos using time-lapse assessment. In this study, fetal bovine serum (FBS), amino acids (AA), and insulin-transferrin-selenium (ITS) were added individually or in combination to culture day 5 parthenogenetic morulae and early blastocysts to investigate their developmental capacity. We found that FBS-AA-ITS supplementation significantly promoted blastocoel expansion (4.4-fold), accelerated early hatching (at day 6 vs. day 7.5) and improved hatching rates (39.59 ± 4.34% vs. 0 ± 0% on day 7, and 80.02 ± 2.93% vs. 2.78 ± 1.60% on day 8) compared with non-supplemented controls. It also increased the number of trophectoderm (2.5-fold) and inner cell mass (2.7-fold). Furthermore, FBS-AA-ITS elevated BCL2 expression and reduced BAX expression, potentially inhibiting apoptosis. In in vitro fertilized embryos, the combination also significantly improved the hatching rate (8.3-fold) and cell number (2.7-fold). Analysis of the effects of FBS on hatching dynamics revealed that FBS enhanced hatching by increasing the number of blastomeres and blastocoel diameter. Blastocysts supplemented with FBS had significantly smaller hatching sizes (320.09 ± 5.01 µm vs. 343.95 ± 5.67 µm) and faster rate of hatching progress (5.6-fold) compared with non-FBS groups. In conclusion, FBS-AA-ITS played a pivotal role in supporting the development of late-stage porcine embryos and significantly accelerated their progression through the embryonic developmental phase.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of porcine polyspermy using mechanical vibrations during in vitro fertilization.

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-02-10 DOI: 10.1262/jrd.2024-042

Takehiro Himaki, Kohei Shinada, Asumi Yaegashi

引用次数: 0

The imprinted Igf2-Igf2r axis is critical for exosome biogenesis during the early development of bovine placenta. 印迹Igf2-Igf2r轴在牛胎盘早期发育过程中对外泌体的生物发生至关重要。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-02-05 Epub Date: 2024-12-12 DOI: 10.1262/jrd.2024-081

Kunhua Zheng, Longfei Xiao, Naihan Yuan, Xihui Sheng, Xiaolong Qi, Yingqiu Wang, Chang Chen, Kaijun Guo, Lin Yang, Bingying Liu, Xiangguo Wang

{"title":"The imprinted Igf2-Igf2r axis is critical for exosome biogenesis during the early development of bovine placenta.","authors":"Kunhua Zheng, Longfei Xiao, Naihan Yuan, Xihui Sheng, Xiaolong Qi, Yingqiu Wang, Chang Chen, Kaijun Guo, Lin Yang, Bingying Liu, Xiangguo Wang","doi":"10.1262/jrd.2024-081","DOIUrl":"10.1262/jrd.2024-081","url":null,"abstract":"Insulin-like growth factor 2 (IGF2) is essential for cell growth and differentiation and functions through the IGF2 receptor (IGF2R) to regulate embryonic and placental development. Exosomes that are synthesized and released from cells and play important roles in embryogenesis and placental development rely on the IGF2R for sorting and transport. However, the role of the imprinted Igf2-Igr2r axis and exosomes in the co-regulation of early placental development remains unknown. Cotyledon villi were collected from bovine placentas at different gestational ages, and the localization and expression of IGF2, IGF2R, and exosomal marker proteins were detected. Furthermore, the expression of exosomal marker factors was detected after the expression of IGF2R or IGF2 was inhibited through RNA interference or the addition of inhibitors, respectively. Our results demonstrated that IGF2, IGF2R, and the exosomal markers CD63, CD9, TSG101, and Rab11 are mainly located on the cell membrane of mononuclear trophoblast cells and binuclear trophoblast cells, which make up the cotyledon villi of the bovine placenta. The expressions of IGF2, IGF2R, and the exosomal marker proteins CD63, CD9, TSG101, and Rab11 showed a significant upward trend with increased gestation duration. Additionally, both Igf2r-knockdown and suppressing the expression of IGF2 with chromeceptin (IGF2 inhibitor) led to the downregulation of exosomal marker proteins in both bovine placental trophoblast cells (BTCs) and BTC-derived exosomes. Our study confirmed that the imprinted Igf2-Igf2r axis participates in the early development of cotyledon villi in the bovine placenta by manipulating exosome biogenesis, providing evidence for improving disorders during placental development.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"41-48"},"PeriodicalIF":1.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of ovum pick-up-in vitro fertilization and in vitro growth of immature oocytes in ruminants. 反刍动物体外受精取卵及未成熟卵母细胞体外生长的优化。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-02-05 Epub Date: 2024-12-22 DOI: 10.1262/jrd.2024-091

Kenichiro Sakaguchi

{"title":"Optimization of ovum pick-up-in vitro fertilization and in vitro growth of immature oocytes in ruminants.","authors":"Kenichiro Sakaguchi","doi":"10.1262/jrd.2024-091","DOIUrl":"10.1262/jrd.2024-091","url":null,"abstract":"Due to the strong demand for embryo production from young and genotyped superior animals using ovum-pick up (OPU) combined with in vitro fertilization (IVF), the number of in vitro-produced embryos has exceeded that of in vivo-derived embryos globally since 2016. One of the merits of OPU-IVF is that the administration of follicle-stimulating hormone (FSH) is not essential, while FSH treatment prior to OPU promotes oocyte developmental competence. Thus, investigations are needed to optimize OPU-IVF protocols with and without FSH. In addition, OPU enables oocyte collection from antral follicles in living animals. However, there are numerous immature oocytes in follicles at earlier stages, which are potentially destined to degenerate in ovaries. The technology used to foster acquisition of maturational and developmental competences in these immature oocytes is called in vitro growth (IVG). IVG is expected to contribute to assisted reproductive technologies for livestock, humans, and endangered species. However, no offspring from preantral follicles has been reported using IVG in animals other than in mice. Furthermore, IVG can be used to investigate factors affecting the fertility and developmental competence of oocytes by reconstituting follicle growth at each stage in vitro, which cannot be evaluated in vivo. Here, the technological progress of the optimization of immature bovine oocyte utilization is reviewed alongside findings from a variety of other ruminants.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"1-9"},"PeriodicalIF":1.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semen extender triggers a mild physiological inflammatory response in the uterus without disrupting sperm-uterine immune crosstalk in vitro in cattle. 精液扩展剂在不破坏牛体外精子-子宫免疫串扰的情况下引发子宫内轻度生理炎症反应。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-02-05 Epub Date: 2024-12-09 DOI: 10.1262/jrd.2024-093

Malinda Hulugalla, Alireza Mansouri, Elham Waehama, Ihshan Akthar, Akio Miyamoto

{"title":"Semen extender triggers a mild physiological inflammatory response in the uterus without disrupting sperm-uterine immune crosstalk in vitro in cattle.","authors":"Malinda Hulugalla, Alireza Mansouri, Elham Waehama, Ihshan Akthar, Akio Miyamoto","doi":"10.1262/jrd.2024-093","DOIUrl":"10.1262/jrd.2024-093","url":null,"abstract":"Artificial insemination (AI) in cattle involves introducing frozen-thawed sperm, a minimal amount of seminal plasma, and a significant volume of semen extender (SE) into the uterus. Previous studies have demonstrated that sperm interact with bovine endometrial epithelia via TLR 2/1, triggering a weak inflammatory response to clear the endometrium. This study investigated the impact of the major component of the insemination dose, egg yolk-based SE, on the uterine immune response in vitro. The results showed that SE did not affect sperm kinetic parameters or the entry of sperm into the uterine glands. SE alone significantly upregulated the mRNA expression of inflammatory cytokines (NFKB2, TNF, IL1B, CXCL8), TLR2/1, and the inflammasome NLRP3, while downregulating NOD1. Immunofluorescence analyses confirmed the upregulation of the strong inflammatory marker TNF alongside TLR2 and the downregulation of NOD1 in the uterine epithelium, similar to the effects observed with sperm. When combined with sperm, SE did not enhance the protein or mRNA expression of these markers, except for IL1B and CXCL8. In silico analyses revealed a strong affinity between triglycerides (the primary components of egg yolk) and TLR2/1, suggesting a potential role in stabilizing heterodimerization. These findings demonstrate that egg yolk-based SE, independent of sperm, triggers a mild physiological inflammatory response mediated by the TLR2/1 and NOD1 signaling pathways. The suppression of NOD1 by sperm and SE ensures a controlled and weak immune response in the uterus. Notably, despite the SE-induced inflammation, the sperm-uterine immune crosstalk was not disrupted, suggesting that SE does not negatively impact the physiological interactions between sperm and the uterus during AI.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"24-34"},"PeriodicalIF":1.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial insemination of bottlenose dolphins (Tursiops truncatus): A trial with simple instruments based on criteria for estrous behaviors linked to changes in estradiol levels and follicle development. 瓶鼻海豚（Tursiops truncatus）的人工授精：根据与雌二醇水平变化和卵泡发育相关的发情行为标准，使用简单工具进行的试验。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-02-05 Epub Date: 2024-12-15 DOI: 10.1262/jrd.2024-065

Shusaku Sawa, Narumi Kawahiro, Minami W Okuyama

{"title":"Artificial insemination of bottlenose dolphins (Tursiops truncatus): A trial with simple instruments based on criteria for estrous behaviors linked to changes in estradiol levels and follicle development.","authors":"Shusaku Sawa, Narumi Kawahiro, Minami W Okuyama","doi":"10.1262/jrd.2024-065","DOIUrl":"10.1262/jrd.2024-065","url":null,"abstract":"Herein, we report a case of pregnancy of a female bottlenose dolphin (Tursiops truncatus) that was subjected to artificial insemination (AI) in water based on its estrous behavior using simple instruments. AI was performed on this female dolphin once or twice daily for 4 days at the detection of estrous behavior, such as floating horizontally and showing reduced responsiveness, likely indicating the appropriate timing for AI. The female was placed in supine a position in the water to position the genital slit above the water surface. A Nélaton catheter (Fr. 10, 40 cm length), with its tip modified, was inserted approximately 20 cm into the vagina through the genital slit, and 1-2 ml of fresh semen was injected. The AI procedure was performed within 1 min by two technicians. Thus, this AI method may be a new choice for artificial reproduction, as pregnancy success can be achieved with relatively less cost, less difficulty, and less invasive treatments of cetaceans.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"62-67"},"PeriodicalIF":1.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can Humanity Thrive Beyond the Galaxy? 人类能在银河系之外茁壮成长吗？

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2025-02-05 Epub Date: 2024-12-29 DOI: 10.1262/jrd.2024-099

Sayaka Wakayama, Teruhiko Wakayama

{"title":"Can Humanity Thrive Beyond the Galaxy?","authors":"Sayaka Wakayama, Teruhiko Wakayama","doi":"10.1262/jrd.2024-099","DOIUrl":"10.1262/jrd.2024-099","url":null,"abstract":"In the future, human beings will surely expand into space. But given its unique risks, will humanity thrive in space environments? For example, when humans begin living and reproducing in space habitats or on other planets in the solar system, are there risks that future generations may suffer from adverse mutations induced by space radiation, or that embryos and fetuses will develop abnormally in gravitational environments that differ from that of Earth? Moreover, human expansion to other stellar systems requires that for each breed of animal, thousands of individuals must be transported to destination planets to prevent populations from experiencing inbreeding-related degeneration. In even more distant future, when humans have spread throughout the galaxy, all genetic resources on Earth, the planet where humans originated, must be permanently and safely stored- but is this even possible? Such issues with future space colonization may not be an urgent research priority, but research and technological development accompanying advancements in spaceflight will excite many people and contribute to technological improvements that can improve living standards in the present day (e.g., more effective treatments for infertility, etc.). This review will therefore focus primarily on issues related to mammalian reproduction in space environments.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"10-16"},"PeriodicalIF":1.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0