Zinc transporter ZnT3/Slc30a3 has a potential role in zinc ion influx in mouse oocytes

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-23 DOI:10.1262/jrd.2024-044

Atsuko KAGEYAMA, Jumpei TERAKAWA, Shunsuke TAKARABE, Hibiki SUGITA, Yui KAWATA, Junya ITO, Naomi KASHIWAZAKI

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Abstract

Zinc is an essential trace element for various physiological functions, including reproduction. The influx/efflux of zinc ions is regulated by zinc transporters (Zip1–14 and ZnT1–8, 10). However, the precise roles of zinc transporters and zinc dynamics in reproductive functions are unknown. In this study, ZnT3/Slc30a3 gene knockout (KO) mice were used to analyze the role of ZnT3. In ZnT3 KO mice, intracellular zinc ions in oocytes/zygotes were significantly reduced compared to those in controls, and free zinc ions did not accumulate in the oocyte cytoplasm. However, fertilization of these oocytes and the average litter size were comparable to those of control mice. Our results suggest that ZnT3 plays an important role in the accumulation of zinc ions in oocytes but not in the developmental ability of mice. ZnT3 KO mice will be useful for examining zinc dynamics in oocytes and other tissues.

Graphical Abstract Fullsize Image

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锌转运体 ZnT3/Slc30a3 在小鼠卵母细胞锌离子流入中的潜在作用

锌是包括生殖在内的各种生理功能所必需的微量元素。锌离子的流入/流出受锌转运体（Zip1-14 和 ZnT1-8，10）的调控。然而，锌转运体和锌动态在生殖功能中的确切作用尚不清楚。本研究利用 ZnT3/Slc30a3 基因敲除（KO）小鼠来分析 ZnT3 的作用。与对照组相比，ZnT3 KO小鼠卵母细胞/合子细胞内锌离子显著减少，游离锌离子没有在卵母细胞胞质中积累。然而，这些卵母细胞的受精率和平均产仔数与对照组小鼠相当。我们的研究结果表明，ZnT3 在锌离子在卵母细胞中的积累中起着重要作用，但对小鼠的发育能力没有影响。ZnT3 KO小鼠将有助于研究锌在卵母细胞和其他组织中的动态变化。图文摘要全尺寸图片

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.