Carnosine supplementation in cryopreservation solution improved frozen-thawed bovine embryo viability

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-12 DOI:10.1262/jrd.2023-071

Toshimichi ISHII, Kento MORI-KOBAYASHI, Sho NAKAMURA, Satoshi OHKURA, Shuichi MATSUYAMA

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Abstract

Cryopreservation adversely affects embryo quality and viability in vitro.We investigated the effects of cryopreservation solutions supplemented with the antioxidant carnosine on frozen-thawed bovine embryo viability. Bovine blastocysts were produced in vitro and cryopreserved using slow freezing. The rates of re-expanded and hatched blastocysts in the 50 μg/ml carnosine-supplemented group at 4, 24, and 48 h after thawing were higher than those in the control (P< 0.05) group. In frozen-thawed embryos, cryopreservation solution supplemented with carnosine (50 μg/ml) significantly reduced reactive oxygen species (ROS) production(P < 0.05), decreased TUNEL-positive apoptotic cells (P< 0.05), and increased the mRNA expression of BCL2 (P< 0.05), an apoptosis suppressor gene. The expression of translocase of outer mitochondrial membrane 20 (TOMM20), which is involved in protein mitochondrial transport, in the carnosine (50 μg/ml)-treated embryos was significantly higher than that in the control group (P < 0.05). ATP production in frozen-thawed embryos in the 50 μg/ml carnosine-supplemented group was significantly higher than that in the control group (P< 0.05), however no significant difference in the total number of cells per embryo among the groups was observed. These results suggest that supplementing the cryopreservation solution with carnosine can improve the viability of frozen-thawed bovine embryos by reducing oxidative damage.

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在冷冻保存液中添加肉碱可提高冷冻解冻牛胚胎的存活率

我们研究了添加了抗氧化剂肌肽的冷冻保存溶液对冷冻解冻牛胚胎存活率的影响。我们研究了添加了抗氧化剂肌肽的冷冻保存液对冷冻解冻牛胚胎存活率的影响。解冻后 4、24 和 48 小时，50 μg/ml 肌肽补充组的囊胚再膨大率和孵化率均高于对照组（P< 0.05）。在冷冻解冻的胚胎中，添加肌肽（50 μg/ml）的冷冻保存液能显著减少活性氧（ROS）的产生（P< 0.05），减少 TUNEL 阳性的凋亡细胞（P< 0.05），增加细胞凋亡抑制基因 BCL2 的 mRNA 表达（P< 0.05）。参与线粒体蛋白转运的线粒体外膜转运酶 20（TOMM20）在肌肽（50 μg/ml）处理组胚胎中的表达量明显高于对照组（P< 0.05）。补充 50 μg/ml 肌肽组冻融胚胎的 ATP 生成量明显高于对照组（P< 0.05），但各组间每个胚胎的细胞总数无明显差异。这些结果表明，在冷冻保存液中添加肌肽可减少氧化损伤，从而提高冷冻解冻牛胚胎的存活率。图文摘要全尺寸图片

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.