Journal of Reproduction and Development最新文献_第6页

Equine chorionic gonadotropin treatment and timed artificial insemination for dairy cow production under heat stress 马绒毛膜促性腺激素治疗和定时人工授精用于热应激下的奶牛生产

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-12-30 DOI: 10.1262/jrd.2023-069

Daisuke FUNAKOSHI, Hidetoshi SHIOTANI, Makoto SEKI

{"title":"Equine chorionic gonadotropin treatment and timed artificial insemination for dairy cow production under heat stress","authors":"Daisuke FUNAKOSHI, Hidetoshi SHIOTANI, Makoto SEKI","doi":"10.1262/jrd.2023-069","DOIUrl":"https://doi.org/10.1262/jrd.2023-069","url":null,"abstract":"This study investigated the effects of timed artificial insemination (TAI) and equine chorionic gonadotropin (eCG) administration on lactating dairy cows under heat-stress conditions (average temperature-humidity index: 80). Timed artificial insemination was performed on the cows with (n = 57) or without (control, n = 41) supplementation with 500 IU of eCG at the day of PGF2α treatment using the CIDR-Ovsynch protocol. GnRH was administered, and a progesterone device (CIDR) was inserted on Day −10 of the treatment protocol. The CIDR was removed on Day −3, and the cows were treated with PGF2α. Two days later, a 2nd GnRH injection was administered. Subsequently, AI was performed on Day 0 (16–20 h after the 2nd GnRH injection), and pregnancy was diagnosed on Days 32 and 60. Plasma progesterone (P4) concentrations were measured after AI. Results showed that the eCG group had a higher pregnancy per AI (P/AI) than the control group (43.9 vs. 12.2%, P = 0.002), which was also accompanied by elevated P4 levels. Four cows in the eCG group had multiple calves, representing 7.0 and 16.0% of the group and pregnant cows, respectively. In conclusion, 500 IU of eCG combined with CIDR-Ovsynch in lactating dairy cows under severe heat stress conditions successfully improved fertility. However, the protocol may have a slight risk of multiple births. \u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-069/figure/advpub_2023-069.png\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139067097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a fluorometric Cuboni test for the semi-quantitative measurement of urinary estrogen levels and pregnancy detection in mares 开发用于半定量检测母马尿液雌激素水平和妊娠检测的 Cuboni 荧光检测法

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-12-29 DOI: 10.1262/jrd.2023-083

Kaede ODA, Maya YOSHIDA, Abdul Razaq IRSHAD, Tomomi KANAZAWA, Toru TAKAHASHI

{"title":"Development of a fluorometric Cuboni test for the semi-quantitative measurement of urinary estrogen levels and pregnancy detection in mares","authors":"Kaede ODA, Maya YOSHIDA, Abdul Razaq IRSHAD, Tomomi KANAZAWA, Toru TAKAHASHI","doi":"10.1262/jrd.2023-083","DOIUrl":"https://doi.org/10.1262/jrd.2023-083","url":null,"abstract":"The aim of the present study was to develop a semi-quantitative urine pregnancy test for mares based on the Cuboni reaction and to verify the reliability of this test. The urine specimens were hydrolyzed by heating in the presence of hydrochloric acid. The resulting free estrogen were extracted from the urine matrix using toluene. Sulfuric acid was added to the toluene extract and the mixture was heated again. The lower layer in the test tube containing sulfuric acid was used for fluorescence measurements with excitation at 355 nm and measurement at 535 nm. The fluorometric Cuboni test revealed that the fluorescence counts in urine samples collected after the second trimester of gestation were significantly higher than those obtained from barren mares. The levels of estrogens, including equilin, estrone and estardiol-17β exhibited a dose-dependent increase in fluorescence counts, whereas other steroids, such as progesterone, testosterone, and cortisol, did not affect fluorescence. Heat treatment of urine samples with hydrochloric acid significantly increased the fluorescence counts in those collected after the second trimester of gestation compared to non-pregnant samples, implying the presence of large amounts of conjugated estrogens in pregnant mare urine. Fluorescence counts in urine samples obtained during pregnancy showed a positive relationship with estrone concentrations as measured by enzyme immunoassay. The results of the present study showed that the fluorometric Cuboni test facilitates urine fluorescence counts depending on the urinary estrogen content and is capable of discriminating between pregnancy and non-pregnancy states beyond the second trimester of gestation in mares.\u0000","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139067138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Negative photoperiod induces an increase in the number of ovulations in dairy cattle 负光周期诱导奶牛增加排卵次数

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-12-29 DOI: 10.1262/jrd.2023-075

Fernando LÓPEZ-GATIUS

{"title":"Negative photoperiod induces an increase in the number of ovulations in dairy cattle","authors":"Fernando LÓPEZ-GATIUS","doi":"10.1262/jrd.2023-075","DOIUrl":"https://doi.org/10.1262/jrd.2023-075","url":null,"abstract":"This study sought to examine the impact of negative photoperiod on the incidence of multiple ovulations and pregnancies in dairy cattle. The study population consisted of 5,373 pregnant cows in their third or greater lactation that experienced their first post-partum pregnancy after spontaneous estrus. The positive photoperiod (increasing day-length) extends from December 22 to June 21, whereas the negative photoperiod (decreasing day-length) extends from June 22 to December 21. The odds ratios (ORs) for multiple ovulations and pregnancies in cows that became pregnant during the negative photoperiod and the remaining cows that became pregnant during the positive photoperiod were 1.4 and 1.3 (P < 0.0001), respectively. The ORs for cows that became pregnant ≥ 90 days in milk and the remaining cows that became pregnant < 90 days in milk were 4.3 and 4.1 (P < 0.0001), respectively. No significant differences were detected in the monthly rates of multiple ovulations or pregnancies during positive and negative photoperiods. Thus, the present study demonstrates that the ovarian function in cows is related to changes in day-length, with decreasing day-length being associated with greater multiple ovulation and pregnancy rates. The present study also shows that positive and negative photoperiods exhibit different trends. The results of this study are consistent with a growing body of work demonstrating the effects of photoperiod patterns on the reproductive physiology of cows, with clear implications for twin pregnancy prevention.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-075/figure/advpub_2023-075.png\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139067690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control 体外精子发生的改进：氧气浓度、抗氧化剂、组织形式设计和空间控制

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-12-23 DOI: 10.1262/jrd.2023-093

Takehiko OGAWA, Takafumi MATSUMURA, Tatsuma YAO, Hiroshi KIMURA, Kiyoshi HASHIMOTO, Yu ISHIKAWA-YAMAUCHI, Takuya SATO

{"title":"Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control","authors":"Takehiko OGAWA, Takafumi MATSUMURA, Tatsuma YAO, Hiroshi KIMURA, Kiyoshi HASHIMOTO, Yu ISHIKAWA-YAMAUCHI, Takuya SATO","doi":"10.1262/jrd.2023-093","DOIUrl":"https://doi.org/10.1262/jrd.2023-093","url":null,"abstract":"Incorporation of bovine serum-derived albumin formulation (AlbuMAX) into a basic culture medium, MEMα, enables the completion of in vitro spermatogenesis through testicular tissue culture in mice. However, this medium was not effective in other animals. Therefore, we sought an alternative approach for in vitro spermatogenesis using a synthetic medium without AlbuMAX and aimed to identify its essential components. In addition to factors known to be important for spermatogenesis, such as retinoic acid and reproductive hormones, we found that antioxidants (vitamin E, vitamin C, and glutathione) and lysophospholipids are vital for in vitro spermatogenesis. Moreover, based on our experience with microfluidic devices (MFD), we developed an alternative approach, the PDMS-ceiling method (PC method), which involves simply covering the tissue with a flat chip made of PDMS, a silicone resin material used in MFD. The PC method, while straightforward, integrates the advantages of MFD, enabling improved and uniform oxygen and nutrient supply via tissue flattening. Furthermore, our studies underscored the significance of lowering the oxygen concentration to 10–15%. Using an integrated cultivation method based on these findings, we successfully achieved in vitro spermatogenesis in rats, which has been a long-standing challenge. Further improvements in culture conditions would pave the way for spermatogenesis completion in diverse animal species. \u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-093/figure/advpub_2023-093.png\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139024107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid droplet formation is spatiotemporally regulated in oocytes during follicular development in mice 小鼠卵泡发育过程中卵母细胞中脂滴的形成受时空调控

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-12-17 DOI: 10.1262/jrd.2023-055

Ryutaro AIZAWA, Megumi IBAYASHI, Junichiro MITSUI, Satoshi TSUKAMOTO

{"title":"Lipid droplet formation is spatiotemporally regulated in oocytes during follicular development in mice","authors":"Ryutaro AIZAWA, Megumi IBAYASHI, Junichiro MITSUI, Satoshi TSUKAMOTO","doi":"10.1262/jrd.2023-055","DOIUrl":"https://doi.org/10.1262/jrd.2023-055","url":null,"abstract":"Communication between oocytes and the surrounding granulosa cells during follicular development is essential for complete oocyte growth. Oocytes contain lipid droplets (LDs), organelles assembled in the endoplasmic reticulum (ER) that store neutral lipids, including triglycerides and cholesterol esters. Although the LD content varies among animals, LDs stored in oocytes have been shown to play an important role in oocyte maturation and preimplantation embryonic development. However, knowledge is lacking regarding how and when LDs are initially produced in developing oocytes within follicles. In the present study, we found that LDs appeared in mouse oocytes in a specific phase during follicular development. The emergence of LDs in intrafollicular oocytes was induced within a similar time window in vitro and in vivo. Fluorescence imaging and electron microscopy revealed that LDs emerging in oocytes during the early stages of follicular growth were in close proximity to the ER. Furthermore, fatty-acid-tracking experiments have revealed that exogenous fatty acids are rapidly incorporated into oocytes, and their uptake is regulated by the interaction between oocytes and granulosa cells, likely in part through transzonal projections. In summary, our results suggest that LD synthesis observed in growing oocytes is spatiotemporally regulated and that oocyte–granulosa cell contact may be involved in LD biosynthesis during follicular development.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-055/figure/advpub_2023-055.jpg\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138692066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluctuation of CD9/SOX2-positive cell populations during the turnover of GH- and TSH-producing cells in the adult anterior pituitary gland. 成人垂体前叶GH和TSH产生细胞转换过程中CD9/SOX2阳性细胞群的波动。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-12-08 Epub Date: 2023-09-30 DOI: 10.1262/jrd.2023-023

Kotaro Horiguchi, Yuto Tsutsui, Ken Fujiwara, Takehiro Tsukada, Takashi Nakakura, Saishu Yoshida, Rumi Hasegawa, Shu Takigami

{"title":"Fluctuation of CD9/SOX2-positive cell populations during the turnover of GH- and TSH-producing cells in the adult anterior pituitary gland.","authors":"Kotaro Horiguchi, Yuto Tsutsui, Ken Fujiwara, Takehiro Tsukada, Takashi Nakakura, Saishu Yoshida, Rumi Hasegawa, Shu Takigami","doi":"10.1262/jrd.2023-023","DOIUrl":"10.1262/jrd.2023-023","url":null,"abstract":"The adenohypophysis is comprised of the anterior and intermediate lobes (AL and IL, respectively). Cluster of differentiation 9 (CD9)- and sex-determining region Y-box 2 (SOX2)-positive cells are stem/progenitor hormone-producing cells in the AL. They are located in the marginal cell layer (MCL) facing Rathke's cleft between the AL and IL (primary niche) and the parenchyma of the AL (secondary niche). We previously showed that, in rats, CD9/SOX2-positive cells in the IL side of the MCL (IL-side MCL) migrate to the AL side (AL-side MCL) and differentiate into prolactin-producing cells (PRL cells) in the AL parenchyma during pregnancy, lactation, and diethylstilbestrol treatment, all of which increase PRL cell turnover. This study examined the changes in CD9/SOX2-positive stem/progenitor cell niches and their proportions by manipulating the turnover of growth hormone (GH)- and thyroid-stimulating hormone (TSH)-producing cells (GH and TSH cells, respectively), which are Pit1 lineage cells, as well as PRL cells. After induction, the isolated CD9/SOX2-positive cells from the IL-side MCL formed spheres and differentiated into GH and TSH cells. We also observed an increased GH cell proportion upon treatment with GH-releasing hormone and recovery from continuous stress and an increased TSH cell proportion upon propylthiouracil treatment, concomitant with alterations in the proportion of CD9/SOX2-positive cells in the primary and secondary niches. These findings suggest that CD9/SOX2-positive cells have the potential to supply GH and TSH when an increase in GH and TSH cell populations is required in the adult pituitary gland.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41148182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation. FABP4介导内质网应激和自噬，以调节绵羊妊娠早期的子宫内膜上皮细胞功能。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-12-08 Epub Date: 2023-10-02 DOI: 10.1262/jrd.2023-015

Meijun Song, Kexing Hao, Fenghua Qi, Wenjuan Zhao, Zhengrong Wang, Jing Wang, Guangdong Hu

{"title":"FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation.","authors":"Meijun Song, Kexing Hao, Fenghua Qi, Wenjuan Zhao, Zhengrong Wang, Jing Wang, Guangdong Hu","doi":"10.1262/jrd.2023-015","DOIUrl":"10.1262/jrd.2023-015","url":null,"abstract":"Dynamic changes in the endometrium are crucial for establishing early pregnancy in ruminants. Blastocyst elongation and implantation require hormones and nutrients to be secreted from the maternal endometrium. The fatty acid-binding protein FABP4 is a widely expressed fatty acid transport protein that promotes cell proliferation, migration, and invasion and is involved in conceptus implantation. However, the mechanism underlying the functional regulation of endometrial epithelial cells (EECs) by FABP4 during ovine peri-implantation remains unclear. We simulated hormonal changes in vitro in sheep EECs (SEECs) during the peri-implantation period and found that it elevated FABP4 expression. FABP4 inhibition significantly reduced cell migration, endoplasmic reticulum stress, and autophagy, suggesting that FABP4 regulates endometrial function in sheep. Moreover, the FABP4 inhibitor BMS309403 counteracted hormone-mediated functional changes in SEECs, and an endoplasmic reticulum stress activator and autophagy inhibitor reversed the abnormal secretion of prostaglandins induced by FABP4 inhibition. These results suggest that FABP4 affects ovine endometrial function during early gestation by regulating endoplasmic reticulum stress and autophagy in SEECs.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41119030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of insulin-like growth factor-1 on the mRNA expression of estradiol receptors, steroidogenic enzymes, and steroid production in bovine follicles. 胰岛素样生长因子-1对牛卵泡雌二醇受体、类固醇生成酶和类固醇产生的mRNA表达的影响。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-12-08 Epub Date: 2023-11-08 DOI: 10.1262/jrd.2023-047

Ahmad Farid Rawan, Hikmatullah Langar, Maho Munetomo, Yuki Yamamoto, Kohei Kawano, Koji Kimura

{"title":"Effects of insulin-like growth factor-1 on the mRNA expression of estradiol receptors, steroidogenic enzymes, and steroid production in bovine follicles.","authors":"Ahmad Farid Rawan, Hikmatullah Langar, Maho Munetomo, Yuki Yamamoto, Kohei Kawano, Koji Kimura","doi":"10.1262/jrd.2023-047","DOIUrl":"10.1262/jrd.2023-047","url":null,"abstract":"Insulin-like growth factor-1 (IGF-1) plays a crucial role in follicular growth and stimulates steroid hormone production in bovine follicles. Steroid hormones are synthesized through the actions of steroidogenic enzymes, specifically STAR, CYP11A1, HSD3B, and CYP19A1 in both theca cells (TCs) and granulosa cells (GCs), under the influence of gonadotropins. Particularly, estradiol 17β (E2) assumes a central role in follicular development and selection by activating estrogen receptors β (ESR2) in GCs. We assessed ESR2 mRNA expression in GCs of developing follicles and investigated the impact of IGF-1 on the mRNA expression of ESR2, CYP19A1, FSHR, and LHCGR, STAR, CYP11A1, and HSD17B in cultured GCs and TCs, respectively. Additionally, we assessed the influence of IGF-1 on androstenedione (A4), progesterone (P4), and testosterone (T) production in TCs. Small-sized follicles (< 6 mm) exhibited the highest levels of ESR2 mRNA expression, whereas medium-sized follicles (7-8 mm) displayed higher levels than large-sized follicles (≥ 9 mm) (P < 0.05). IGF-1 increased the mRNA expression of ESR2, CYP19A1, and FSHR in GCs of follicles of both sizes, except for FSHR mRNA in medium-sized follicles (P < 0.05). IGF-1 significantly elevated mRNA expression of LHCGR, STAR, CYP11A1, and CYP17B in TCs of small- and medium-sized follicles (P < 0.05). Moreover, IGF-1 augmented the production of A4 and P4 but had no impact on T production in TCs of small- and medium-sized follicles. Taken together, our findings indicate that IGF-1 upregulates steroidogenic enzymes and steroid hormone production, underscoring the crucial role of IGF-1 in follicle development and selection.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71521905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of age at first calving with longevity, milk yield, and fertility up to the third lactation in a herd of Holstein dairy cows in Japan. 日本荷斯坦奶牛群第一次产仔时的年龄与寿命、产奶量和生育能力的关系，直到第三次泌乳。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-12-08 Epub Date: 2023-09-29 DOI: 10.1262/jrd.2023-012

Hiromi Kusaka, Takeshi Yamazaki, Minoru Sakaguchi

{"title":"Association of age at first calving with longevity, milk yield, and fertility up to the third lactation in a herd of Holstein dairy cows in Japan.","authors":"Hiromi Kusaka, Takeshi Yamazaki, Minoru Sakaguchi","doi":"10.1262/jrd.2023-012","DOIUrl":"10.1262/jrd.2023-012","url":null,"abstract":"Accelerating age at first calving (AFC) is a strategy for sustainable dairy farming, whereas the impact of a reduction in AFC on long-term performance remains unclear. In this study, longevity and milk productivity until the end of the third lactation period were investigated retrospectively according to AFC. A total of 169 cows were categorized according to AFC as young, moderate, old, and very old (< 22.5, 22.5 -< 24.0, 24.0 -< 25.5, and > 25.5 months). The young AFC group had approximately 70 kg lower body weight before first calving (620 vs. 695 kg, P < 0.05) and experienced their first calving approximately 4.2 months earlier than the very old AFC group (21.9 vs. 26.1 months, P < 0.05). The survival rate at the third calving stage was 61% in the young AFC group, which was higher than those in the moderate (42%), old (35%), and very old (33%) AFC groups. In the young AFC group, no cows were culled because of low productivity and hoof disease, compared to 5.0-8.1% of older AFC cows. The young AFC group had a higher overall lifetime milk yield (cumulative milk yield/days from birth to the end of final lactation) than the old AFC group (14.3 vs. 8.7 kg/d, P = 0.11). The cows that survived the third calving had better reproductive performance than non-surviving cows; however, no statistical difference was detected among the AFC groups. In conclusion, AFC as early as 22.5 months could be associated with better survivability and higher overall lifetime milk yield than older AFC without impairing reproductive performance. Our results suggest that accelerating AFC may lead to higher profitability.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41125574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accumulation of senescent cells in the stroma of aged mouse ovary. 衰老细胞在衰老小鼠卵巢间质中的积聚。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2023-12-08 Epub Date: 2023-11-06 DOI: 10.1262/jrd.2023-021

Natsumi Maruyama, Isuzu Fukunaga, Tomoaki Kogo, Tsutomu Endo, Wataru Fujii, Masami Kanai-Azuma, Kunihiko Naito, Koji Sugiura

{"title":"Accumulation of senescent cells in the stroma of aged mouse ovary.","authors":"Natsumi Maruyama, Isuzu Fukunaga, Tomoaki Kogo, Tsutomu Endo, Wataru Fujii, Masami Kanai-Azuma, Kunihiko Naito, Koji Sugiura","doi":"10.1262/jrd.2023-021","DOIUrl":"10.1262/jrd.2023-021","url":null,"abstract":"Senescent cells play a detrimental role in age-associated pathogenesis by producing factors involved in senescence-associated secretory phenotype (SASP). The present study was conducted to examine the possibility that senescent cells are present in aged ovaries and, if so, to determine the tissue region where senescent cells accumulate using a mouse model. Female mice at 2-4 and 8-10 months were used as reproductively young and aged models, respectively; the latter included mice with and without reproductive experience. Cells positive for senescence-associated β-galactosidase (SA-β-Gal) staining, one of the markers of cellular senescence, were detected in the stromal region of aged, but not young, ovaries regardless of reproductive experience. Likewise, the localization of cells expressing CDKN2A (cyclin dependent kinase inhibitor 2A), another senescence marker, in the stromal region of aged ovaries was detected with immunohistochemistry. CDKN2A expression detected by western blotting was significantly higher in the ovaries of aged mice with reproductive experience than in those without the experience. Moreover, cells positive for both γH2AX (a senescence marker) and fluorescent SA-β-Gal staining were present in those isolated from aged ovaries. In addition, the transcript levels of several SASP factors were significantly increased in aged ovaries. These results suggest that senescent cells accumulate in the ovarian stroma and may affect ovarian function in aged mice. Additionally, reproductive experience may promote accumulation.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71482782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0