Journal of Reproduction and Development最新文献_第6页

Fertilization and developmental competence of in vitro fertilized embryos from C57BL/6J mice of different ages and the impact of vitrification. 不同年龄 C57BL/6J 小鼠体外受精胚胎的受精和发育能力以及玻璃化的影响。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-12-13 Epub Date: 2024-10-25 DOI: 10.1262/jrd.2024-082

Seiji Kito

{"title":"Fertilization and developmental competence of in vitro fertilized embryos from C57BL/6J mice of different ages and the impact of vitrification.","authors":"Seiji Kito","doi":"10.1262/jrd.2024-082","DOIUrl":"10.1262/jrd.2024-082","url":null,"abstract":"Prepubertal animals are often preferred as sources of oocytes for assisted reproductive technologies (ARTs) in laboratory mice, but the normality and developmental competence of these oocytes remain controversial. This study systematically examined in vitro fertilization competence, embryo development, and fetal development after embryo transfer (ET) using oocytes from C57BL/6J mice aged 3 to 10 weeks. Oocytes were collected from superovulated females, fertilized, and cultured in vitro for 96 h or transferred at 2-cell stage to recipient females. Additionally, fetal development was compared between unfrozen and frozen-thawed in vitro-fertilized 2-cell embryos across different age groups. The number of ovulated oocytes per animal decreased with age, while the percentage of morphologically normal oocytes was highest in 3-week-old mice (99%) compared to older ages (70-86%, P < 0.05). Although fertilization percentages were consistently high (≥ 97%), blastocyst development in vitro, the nuclear counts of blastocysts and fetal development after ET were lowest for embryos from 3-week-old mice. Development of frozen-thawed embryos to fetuses was significantly reduced compared to unfrozen embryos in all age groups, except for those from 10-week-old mice. These findings suggest that oocytes from prepubertal mice, particularly from 3-week-old mice, are less developmentally competent than those from older mice. Therefore, the age of animals for oocyte source should be carefully considered based on the specific requirements of the research or ART applications.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"405-410"},"PeriodicalIF":1.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagy in the corpus luteum correlates with tissue growth in pregnant rats. 黄体中的自噬与怀孕大鼠的组织生长有关。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-07-07 DOI: 10.1262/jrd.2024-019

Yasuaki Oishi, Koji Asakawa, Yuri Ishiwata, Shota Oka, Ryota Terashima, Makoto Sugiyama, Keiichiro Kizaki, Mitsumori Kawaminami, Shiro Kurusu

{"title":"Autophagy in the corpus luteum correlates with tissue growth in pregnant rats.","authors":"Yasuaki Oishi, Koji Asakawa, Yuri Ishiwata, Shota Oka, Ryota Terashima, Makoto Sugiyama, Keiichiro Kizaki, Mitsumori Kawaminami, Shiro Kurusu","doi":"10.1262/jrd.2024-019","DOIUrl":"10.1262/jrd.2024-019","url":null,"abstract":"The developmental activation of the corpus luteum (CL) structurally and functionally is critical for the temporally regulated establishment, maintenance, and termination of pregnancy in rats. In this study, we have investigated the possible involvement of autophagy in the regulation of the CL during pregnancy in rats. The expression ratio of microtubule-associated protein light chain 3 (LC3)-II/-I, a widely used indicator of autophagic activity, in the CL remained relatively stable until day 15 of pregnancy. Subsequently, it progressively increased until day 21, and then declined until day 3 postpartum. This fluctuation was closely associated with the tissue weight of the CL rather than progesterone (P4) production activity. Light and electron microscopy revealed the presence of immunoreactive LC3 aggregates and irregularly shaped autolysosome-like microstructures in the cytoplasm of luteal cells during late pregnancy. Notably, a bolus intrabursal injection of the autophagy inhibitor bafilomycin A1 on day 15 of pregnancy resulted in a significant reduction in luteal cell size and disrupted the normal alteration of circulating P4 levels. Consequently, treatment with this inhibitor increased the likelihood of the varied timing (both advanced and delayed) of delivery and led to reduced body weight in neonates when compared with the vehicle-treated control group. Our findings suggest that autophagy in the rat CL contributes to luteal tissue growth, influences P4 production, and thereby fine-tunes the regulation of gestation length in rats.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"286-295"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dairy cow parity affects relationships among nutritional parameters in the blood of dams, umbilical cords, and calves and placental development at calving. 奶牛奇数影响母牛、脐带和犊牛血液中的营养参数与产犊时胎盘发育之间的关系。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-07-07 DOI: 10.1262/jrd.2024-010

Riku Mashimo, Hanon Ohban, Yuka Kumazaki, Sayaka Ito, Tomono Katagiri, Nobuyuki Kusaba, Chiho Kawashima

{"title":"Dairy cow parity affects relationships among nutritional parameters in the blood of dams, umbilical cords, and calves and placental development at calving.","authors":"Riku Mashimo, Hanon Ohban, Yuka Kumazaki, Sayaka Ito, Tomono Katagiri, Nobuyuki Kusaba, Chiho Kawashima","doi":"10.1262/jrd.2024-010","DOIUrl":"10.1262/jrd.2024-010","url":null,"abstract":"Heifer growth and milk production in lactating cows may diminish the nutrient supply to the fetus. This study aimed to analyze the characteristics of the nutrient supply to the fetus in primiparous and multiparous cows. We investigated maternal, umbilical cord, and calf blood glucose and amino acid levels, as well as placental development in 28 primiparous (PP) and 30 multiparous (MP) Holstein cows. Although the total cotyledonary weight and surface area showed no significant differences, the MP group exhibited larger individual cotyledons (P < 0.01) and fewer medium-sized cotyledons (P < 0.05). Within the PP group, total cotyledonary weight and surface area positively correlated with blood glucose (r = 0.71-0.77; P < 0.01) and total essential amino acid (r = 0.55; P < 0.05) concentrations in the umbilical veins. However, no significant correlation was observed in the MP group. Blood glucose and amino acid concentrations in the umbilical vein, umbilical artery, and calf were significantly lower in the MP group (P < 0.05), although no difference was observed in the dams between the groups. In conclusion, the nutrient status of primiparous cows can alter fetal nutrient supply. Moreover, multiparous cows have larger individual cotyledons as an adaptive response to increased milk production during pregnancy. However, this adaptive response in multiparous cows did not completely restore nutrient supply to the fetus to the same extent as that in primiparous cows. Therefore, the nutritional management of multiparous cows during pregnancy must be reconsidered.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"264-271"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cpne1 deficiency preserves sperm motility under Ca²⁺ channel blockade. 在 Ca2+ 通道阻断的情况下，Cpne1 的缺乏可保持精子活力。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-07-14 DOI: 10.1262/jrd.2024-027

Qiang Xie, Hanbin Zhang, Yuge Zhuang, Jinsheng Liu, Zicong Huang, Xiaoyuan Zhang, Ke Ma, Wenyuan Liu, Minyu Xie, Chuyu Huang, Xiaojing Zhong, Feilong Chen, Feng Zou, Wansong Zhang, Chunming Qiu, Canbiao Sun, Xiangjin Kang, Zhenguo Chen, Guofei Zhang

{"title":"Cpne1 deficiency preserves sperm motility under Ca2+ channel blockade.","authors":"Qiang Xie, Hanbin Zhang, Yuge Zhuang, Jinsheng Liu, Zicong Huang, Xiaoyuan Zhang, Ke Ma, Wenyuan Liu, Minyu Xie, Chuyu Huang, Xiaojing Zhong, Feilong Chen, Feng Zou, Wansong Zhang, Chunming Qiu, Canbiao Sun, Xiangjin Kang, Zhenguo Chen, Guofei Zhang","doi":"10.1262/jrd.2024-027","DOIUrl":"10.1262/jrd.2024-027","url":null,"abstract":"Calcium ions (Ca2+) play crucial roles in sperm motility and fertilization. The copine (CPNE) family comprises several Ca2+-dependent phospholipid-binding proteins. Of these, CPNE1 is extensively expressed in mammalian tissues; however, its precise role in testicular development and spermatogenesis is yet to be fully characterized. In this study, we used proteomics to analyze testicular biopsies and found that levels of CPNE1 were significantly reduced in patients with non-obstructive azoospermia (defective spermatogenesis) compared to those in patients with obstructive azoospermia (physiological spermatogenesis). In mice, CPNE1 is expressed at various stages of germ cell development and is associated with the Golgi apparatus. Ultimately, CPNE1 is expressed in the flagella of mature sperms. To further examine the role of CPNE1, we developed a Cpne1 knockout mouse model. Analysis showed that the loss of Cpne1 did not impair testicular development, spermatogenesis, or sperm morphology and motility in physiological conditions. When treated with gadolinium (III) chloride or 2-aminoethoxydiphenyl borate, known inhibitors of store-operated Ca2+ entry, Ca2+ signals and sperm motility were significantly compromised in wild-type mice; however, both mechanisms were conserved in KO mice. These results suggested that CPNE1 is dispensable for testicular development, spermatogenesis or sperm motility in physiological conditions. In addition, CPNE1 may represent a target of Ca2+ channel inhibitors and may therefore be implicated in the regulation of Ca2+ signaling and sperm motility.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"309-319"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perilipin2 depletion causes lipid droplet enlargement in the ovarian corpus luteum in mice. 消耗 Perilipin2 会导致小鼠卵巢黄体脂滴增大。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-07-15 DOI: 10.1262/jrd.2024-023

Megumi Ibayashi, Takayuki Tatsumi, Satoshi Tsukamoto

{"title":"Perilipin2 depletion causes lipid droplet enlargement in the ovarian corpus luteum in mice.","authors":"Megumi Ibayashi, Takayuki Tatsumi, Satoshi Tsukamoto","doi":"10.1262/jrd.2024-023","DOIUrl":"10.1262/jrd.2024-023","url":null,"abstract":"Lipid droplets (LDs) are endoplasmic reticulum-derived organelles that store neutral lipids (mostly triglycerides and cholesterol esters) within a phospholipid monolayer and appear in most eukaryotic cells. Perilipins (PLINs, comprising PLIN1-5) are abundant LD-associated proteins with highly variable expression levels among tissues. Although PLINs are expressed in the mammalian ovaries, little is known about their subcellular localization and physiological functions. In this study, we investigated the localization of PLIN1-3 and their relationship with LD synthesis using mCherry-HPos reporter mice, thereby enabling the visualization of LD biogenesis in vivo. PLIN2 and PLIN3 were localized as puncta in granulosa cells with low levels of LD synthesis in developing follicles. This localization pattern was quite different from that of PLIN1, which was mainly localized in the theca and interstitial cells with high levels of LD synthesis. In the corpus luteum, where LD synthesis is highly induced, PLIN2 and PLIN3 are abundant in the particulate structures, whereas PLIN1 is poorly distributed. We also generated global Plin2-deficient mice using the CRSPR/Cas9 system and demonstrated that the lack of PLIN2 did not alter the distribution of PLIN1 and PLIN3 but unexpectedly induced LD enlargement in the corpus luteum. Collectively, our results suggest that the localization of PLIN1-3 is spatiotemporally regulated and that PLIN2 deficiency influences LD mobilization in the corpus luteum within the ovaries.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"296-302"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Central δ/κ opioid receptor signaling pathways mediate chronic and/or acute suckling-induced LH suppression in rats during late lactation. 中枢δ/κ阿片受体信号通路介导哺乳后期大鼠慢性和/或急性吸吮引起的LH抑制。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-08-16 DOI: 10.1262/jrd.2024-045

Yoshihisa Uenoyama, Miku Nonogaki, Hitomi Tsuchida, Marina Takizawa, Sena Matsuzaki, Naoko Inoue, Hiroko Tsukamura

{"title":"Central δ/κ opioid receptor signaling pathways mediate chronic and/or acute suckling-induced LH suppression in rats during late lactation.","authors":"Yoshihisa Uenoyama, Miku Nonogaki, Hitomi Tsuchida, Marina Takizawa, Sena Matsuzaki, Naoko Inoue, Hiroko Tsukamura","doi":"10.1262/jrd.2024-045","DOIUrl":"10.1262/jrd.2024-045","url":null,"abstract":"In mammals, secretion of tonic (pulsatile) gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) is often suppressed during lactation. Suppression of GnRH/LH pulses in lactating dams is assumed to be caused by suckling stimuli and a chronic negative energy balance due to milk production. The present study aimed to investigate whether the central enkephalin-δ opioid receptor (DOR) signaling mediated the suppression of LH secretion by acute suckling stimuli and/or chronic negative energy balance due to milk production in rats during late lactation when dams were under a heavy energy demand. On postpartum day 16, the number of Penk (enkephalin mRNA)-expressing cells in the arcuate nucleus was significantly higher in lactating rats than in non-lactating control rats. Pulsatile LH secretion was suppressed in rats with chronic suckling or acute 1-h suckling stimuli 6 h after pup removal on day 16 of lactation. Central DOR antagonism significantly increased the mean LH concentrations and the baseline of LH pulses in rats with chronic suckling but not with acute suckling stimuli on day 16 of lactation. Besides, central κ opioid receptor (KOR) antagonism increased the amplitude of LH pulses in rats with the acute suckling stimuli on day 16 of lactation. These results suggest that central DOR signaling mediates the suppression of LH secretion caused by a negative energy balance in rats receiving chronic suckling during late lactation. On the other hand, central KOR signaling likely mediates acute suckling stimuli-induced suppression of LH secretion in rats during late lactation.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"327-337"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kdm4d mutant mice show impaired sperm motility and subfertility. Kdm4d 突变小鼠表现出精子活力受损和不育。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-07-22 DOI: 10.1262/jrd.2024-039

Zhuoran Xu, Yuka Fujimoto, Mizuki Sakamoto, Daiyu Ito, Masahito Ikawa, Takashi Ishiuchi

引用次数: 0

Association between prepartum vaginal temperature changes and placenta expulsion time in Holstein dairy cattle. 荷斯坦奶牛产前阴道温度变化与胎盘排出时间的关系。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-10-01 Epub Date: 2024-07-10 DOI: 10.1262/jrd.2024-012

Miki Sakatani, Kaiyu Kubota, Satoshi Haga, Masafumi Miwa

{"title":"Association between prepartum vaginal temperature changes and placenta expulsion time in Holstein dairy cattle.","authors":"Miki Sakatani, Kaiyu Kubota, Satoshi Haga, Masafumi Miwa","doi":"10.1262/jrd.2024-012","DOIUrl":"10.1262/jrd.2024-012","url":null,"abstract":"Retained placenta (RP) adversely affects postpartum productivity and reproduction in dairy cattle. Thus, methods to predict the occurrence of RP before calving would be desirable. Herein, we assessed whether vaginal temperature measurements (which have already been applied to detect calving) could be used to predict the occurrence of RP in cattle. A vaginal temperature recording device was inserted into the vagina of 49 pregnant Holstein-Friesian heifers (n = 16) and cows (n = 33); this device recorded the vaginal temperature every 5 min until the device dropped out at calving. Serum was collected 10 days before the expected calving date. The time points of calving and placental expulsion were identified via video recordings. We further calculated calving duration (temperature decrease to calving) and placenta expulsion time (PE time = calving to placenta expulsion). The PE times were divided into four categories (0-4 h, 4-8 h, 8-12 h, and RP at >12 h), while subsequent analysis revealed that an extension of the PE time dependent on the shortening of the calving duration (P < 0.05). The vaginal temperature patterns also differed in a PE time-dependent manner, and cows with RP did not show any re-elevation of vaginal temperature. Serum analyses indicated an energy deficiency in RP cattle. These results suggest that RP may be detected early as a specific change in the vaginal temperature associated with reproductive hormone secretion.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"272-278"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional cell culture using CD9-positive cells isolated from marginal cell layer of intermediate lobe of rats sustains in vivo-like primary niche environment 利用从大鼠中叶边缘细胞层分离出的 CD9 阳性细胞进行三维细胞培养，可维持类似于活体原生龛环境的培养效果

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-08-11 DOI: 10.1262/jrd.2024-033

Kotaro HORIGUCHI, Takehiro TSUKADA, Saishu YOSHIDA, Ken FUJIWARA, Takashi NAKAKURA, Morio AZUMA, Ayano SHINDO, Rumi HASEGAWA, Shu TAKIGAMI

{"title":"Three-dimensional cell culture using CD9-positive cells isolated from marginal cell layer of intermediate lobe of rats sustains in vivo-like primary niche environment","authors":"Kotaro HORIGUCHI, Takehiro TSUKADA, Saishu YOSHIDA, Ken FUJIWARA, Takashi NAKAKURA, Morio AZUMA, Ayano SHINDO, Rumi HASEGAWA, Shu TAKIGAMI","doi":"10.1262/jrd.2024-033","DOIUrl":"https://doi.org/10.1262/jrd.2024-033","url":null,"abstract":"The adenohypophysis is composed of the anterior and intermediate lobes (AL and IL, respectively), and secretes hormones that play an important role in reproduction. CD9- and SOX2-double (CD9/SOX2) positive cells located in the marginal cell layer (MCL) facing the Rathke’s cleft in the AL and IL form the primary stem cell niche in the adult adenohypophysis of rats. In this study, we successfully obtained 3-dimensional (3D) cell aggregates that closely resembled the primary niche of MCL in vivo. After incubation in a Matrigel containing several growth factors, approximately 20% of the cells in the CD9/SOX2-positive cell aggregates were differentiated into hormone-producing cells. The cell aggregates generated in this study may provide insight into the regulation of the pituitary stem/progenitor cell niche and the turnover of hormone-producing cells.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2024-033/figure/advpub_2024-033.jpg\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":"22 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141935656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role and action mechanisms of tPA in CRH-induced apoptosis of mouse oviductal epithelial and mural granulosa cells. tPA 在 CRH 诱导的小鼠输卵管上皮细胞和壁层颗粒细胞凋亡中的作用和作用机制。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-08-07 Epub Date: 2024-06-24 DOI: 10.1262/jrd.2024-028

Yong-Qing Yang, Min Zhang, Qi Hua, Rui-Jie Ma, Xiao-Yan Wang, Hong-Jie Yuan, Ming-Jiu Luo, Jing-He Tan

{"title":"Role and action mechanisms of tPA in CRH-induced apoptosis of mouse oviductal epithelial and mural granulosa cells.","authors":"Yong-Qing Yang, Min Zhang, Qi Hua, Rui-Jie Ma, Xiao-Yan Wang, Hong-Jie Yuan, Ming-Jiu Luo, Jing-He Tan","doi":"10.1262/jrd.2024-028","DOIUrl":"10.1262/jrd.2024-028","url":null,"abstract":"Understanding how stress hormones induce apoptosis in oviductal epithelial cells (OECs) and mural granulosa cells (MGCs) can reveal the mechanisms by which female stress impairs embryonic development and oocyte competence. A recent study showed that tissue plasminogen activator (tPA) ameliorates corticosterone-induced apoptosis in MGCs and OECs by acting on its receptors low-density lipoprotein receptor-related protein 1 (LRP1) and Annexin A2 (ANXA2), respectively. However, whether tPA is involved in corticotropin-releasing hormone (CRH)-induced apoptosis and whether it uses the same or different receptors to inhibit apoptosis induced by different hormones in the same cell type remains unknown. This study showed that CRH triggered apoptosis in both OECs and MGCs and significantly downregulated tPA expression. Moreover, tPA inhibits CRH-induced apoptosis by acting on ANXA2 in both OECs and MGCs. While ANXA2 inhibits apoptosis via phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling, LRP1 reduces apoptosis via mitogen-activated protein kinase (MAPK) signaling. Thus, tPA used the same receptor to inhibit CRH-induced apoptosis in both OECs and MGCs, however used different receptors to inhibit corticosterone-induced apoptosis in MGCs and OECs. These data helps understand the mechanism by which female stress impairs embryo/oocyte competence and proapoptotic factors trigger apoptosis in different cell types.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"238-246"},"PeriodicalIF":1.9,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11310383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0