Three-dimensional cell culture using CD9-positive cells isolated from marginal cell layer of intermediate lobe of rats sustains in vivo-like primary niche environment

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-08-11 DOI:10.1262/jrd.2024-033

Kotaro HORIGUCHI, Takehiro TSUKADA, Saishu YOSHIDA, Ken FUJIWARA, Takashi NAKAKURA, Morio AZUMA, Ayano SHINDO, Rumi HASEGAWA, Shu TAKIGAMI

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Abstract

The adenohypophysis is composed of the anterior and intermediate lobes (AL and IL, respectively), and secretes hormones that play an important role in reproduction. CD9- and SOX2-double (CD9/SOX2) positive cells located in the marginal cell layer (MCL) facing the Rathke’s cleft in the AL and IL form the primary stem cell niche in the adult adenohypophysis of rats. In this study, we successfully obtained 3-dimensional (3D) cell aggregates that closely resembled the primary niche of MCL in vivo. After incubation in a Matrigel containing several growth factors, approximately 20% of the cells in the CD9/SOX2-positive cell aggregates were differentiated into hormone-producing cells. The cell aggregates generated in this study may provide insight into the regulation of the pituitary stem/progenitor cell niche and the turnover of hormone-producing cells.

Graphical Abstract Fullsize Image

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利用从大鼠中叶边缘细胞层分离出的 CD9 阳性细胞进行三维细胞培养，可维持类似于活体原生龛环境的培养效果

腺下丘脑由前叶（AL）和中间叶（IL）组成，分泌的激素在生殖过程中发挥着重要作用。CD9和SOX2双（CD9/SOX2）阳性细胞位于AL和IL中面向Rathke裂隙的边缘细胞层（MCL），构成了成年大鼠腺下丘脑的初级干细胞龛。在这项研究中，我们成功获得了与体内MCL原生龛非常相似的三维（3D）细胞聚集体。在含有多种生长因子的 Matrigel 中培养后，CD9/SOX2 阳性细胞聚集体中约有 20% 的细胞分化为激素分泌细胞。本研究中产生的细胞聚集体可能有助于深入了解垂体干/祖细胞生态位的调控和激素产生细胞的更替。图文摘要全尺寸图片

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.