Journal of Reproduction and Development最新文献_第5页

Optimizing lifetime productivity in high-producing dairy cows: challenges in the management of age at first calving, double ovulation, and postpartum uterine health. 优化高产奶牛的终身生产力：首次产犊年龄管理、双排卵和产后子宫健康方面的挑战

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-06-07 DOI: 10.1262/jrd.2026-033

Hiromi Kusaka

{"title":"Optimizing lifetime productivity in high-producing dairy cows: challenges in the management of age at first calving, double ovulation, and postpartum uterine health.","authors":"Hiromi Kusaka","doi":"10.1262/jrd.2026-033","DOIUrl":"10.1262/jrd.2026-033","url":null,"abstract":"The dairy industry has seen remarkable increases in milk yield per cow; however, these gains are often offset by declining fertility and a reduced lifespan. This review discusses strategies to enhance lifetime productivity, focusing on three key research areas: 1) Age at first calving (AFC): Retrospective analysis indicates that reducing AFC to < 22.5 months enhances lifetime daily milk yield without adverse effects through the third lactation, provided that the heifer reaches a body weight of ≥ 600 kg before first calving. 2) Double Ovulation: Sequential ultrasonic observation of the ovaries characterizes the pathophysiology of spontaneous double ovulation in high-producing cows during the early postpartum period. Multiparous cows exhibit a higher incidence of double ovulation that leads to twin pregnancies, which is associated with a negative energy balance. This metabolic state can be monitored using milk components such as lactose as a potential metabolic indicator of follicular dynamics. 3) Postpartum uterine health: Diagnostic strategies for endometritis have been optimized. Integrating vaginal discharge scoring and ultrasonography ensures a reliable assessment. This multimodal approach significantly improves the sensitivity of predicting reproductive performance compared to single-method assessments. Improving the lifetime productivity of modern dairy cattle requires precise management across various life stages. Automation and labor-saving technologies, particularly in routine husbandry, reproductive management, and veterinary practices, will become increasingly important for maintaining sustainable dairy operations.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"581-589"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex determination and genetic screening of equine embryos with whole genome amplification and real-time PCR. 全基因组扩增和实时PCR技术在马胚胎性别鉴定和遗传筛选中的应用。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-03-23 DOI: 10.1262/jrd.2026-014

Gus R McFarlane, Elizabeth Backhouse, Agustin Ruiz, Brendon A O'Rourke, Jenin V Cortez, Christopher G Grupen

{"title":"Sex determination and genetic screening of equine embryos with whole genome amplification and real-time PCR.","authors":"Gus R McFarlane, Elizabeth Backhouse, Agustin Ruiz, Brendon A O'Rourke, Jenin V Cortez, Christopher G Grupen","doi":"10.1262/jrd.2026-014","DOIUrl":"10.1262/jrd.2026-014","url":null,"abstract":"Reliable pre-implantation sex determination and genetic screening enables informed embryo transfer decisions in equine breeding while avoiding later interventions. We developed a streamlined workflow that couples rapid whole genome amplification (WGA) with a multiplex real-time PCR targeting ETSTY5 as a Y-specific marker and UBC as an autosomal control. On purified equine DNA, sex was correctly assigned down to 10 pg gDNA and to a single fibroblast cell. Direct testing of embryo biopsies without WGA yielded inconsistent results, whereas introducing a short WGA step produced tight allelic-discrimination clusters and 100% diagnostic calls, including in cloned embryos of known sex. The same WGA product supported targeted genotyping for inherited disease screening of hyperkalemic periodic paralysis (HYPP) and hereditary equine regional dermal asthenia (HERDA) alleles. This WGA plus real-time PCR pipeline supports robust and practical embryo sexing and targeted pre-implantation genetic diagnostics within in vitro produced equine embryo and embryo transfer workflows.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"719-722"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147499179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preimplantation culture conditions and later-life outcomes: implications for evaluating embryo culture media. 着床前培养条件和后期生活结果：评估胚胎培养基的意义。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-05-01 DOI: 10.1262/jrd.2026-041

Jannatul Ferdous Jharna, Md Wasim Bari, Sakino Furusato, Kaname Sato, Yui Maruhashi, Norermi Firzana Alfian, Masahiro Horizoe, Yoshiya Morishita, Kei Uechi, Satoshi Kishigami

引用次数: 0

Genomic evaluation and gene expression analysis of preimplantation embryos for enhancing cattle breeding and in vitro embryo production. 着床前胚胎的基因组评估和基因表达分析，以提高牛的育种和体外胚胎生产。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-06-05 DOI: 10.1262/jrd.2026-067

Takashi Fujii

{"title":"Genomic evaluation and gene expression analysis of preimplantation embryos for enhancing cattle breeding and in vitro embryo production.","authors":"Takashi Fujii","doi":"10.1262/jrd.2026-067","DOIUrl":"10.1262/jrd.2026-067","url":null,"abstract":"Genomic selection using genomic estimated breeding values (GEBVs) based on single nucleotide polymorphism (SNP) genotyping has been widely adopted for efficient breeding of cattle. Recent advances in DNA analysis technologies have enabled comprehensive SNP genotyping using a small number of cells. Implementation of a preimplantation genomic selection (PGS) system, wherein GEBVs are calculated from a limited number of embryonic cells, can further enhance breeding efficiency through the selective production of genetically superior animals by embryo transfer. In addition, the use of bovine embryos produced by in vitro fertilization (IVF) has increased worldwide in recent years. However, the implantation potential of bovine IVF embryos is lower than that of in vivo-derived (IVD) embryos, and aberrant epigenetic modifications and gene expression are considered major contributing factors to the reduced developmental competence of bovine IVF embryos. Therefore, the differences in the gene expression characteristics of bovine IVD and IVF embryos need to be better understood for improved bovine in vitro production (IVP) systems. This review outlines the development and applications of PGS systems using bovine blastocysts, elongating conceptuses, and demi-embryos derived from blastomere separation. It also discusses the utility of gene expression profiling as a method for evaluating the quality of bovine preimplantation embryos, which may contribute to improvements in bovine IVP systems.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"590-596"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of thiol compounds on DNA-damage detection and in vitro fertilization outcomes in bovine sperm. 巯基化合物对牛精子dna损伤检测和体外受精结果的影响。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-03-05 DOI: 10.1262/jrd.2025-138

Kumiko Takeda, Kazuko Ogata, Kazutsugu Matsukawa, Hiroaki Yokokawa, Yutaka Fukumoto, Kenji Morimasa, Takemasa Hidaka, Takuya Nogi, Kenichi Watabe, Kumi Iijima, Ryoichi Nakamura, Masaki Ozawa, Masahiro Kaneda

{"title":"Effects of thiol compounds on DNA-damage detection and in vitro fertilization outcomes in bovine sperm.","authors":"Kumiko Takeda, Kazuko Ogata, Kazutsugu Matsukawa, Hiroaki Yokokawa, Yutaka Fukumoto, Kenji Morimasa, Takemasa Hidaka, Takuya Nogi, Kenichi Watabe, Kumi Iijima, Ryoichi Nakamura, Masaki Ozawa, Masahiro Kaneda","doi":"10.1262/jrd.2025-138","DOIUrl":"10.1262/jrd.2025-138","url":null,"abstract":"Reduced glutathione (GSH), a thiol compound, plays an important role in protecting sperm from excessive levels of reactive oxygen species. Our previous study demonstrated that GSH supplementation was associated with improved in vitro fertilization (IVF) outcomes. However, GSH supplementation also increased the proportion of DNA-damaged sperm detected in the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay, indicating involvement of its thiol components. In this study, we investigated the effects of several thiol compounds, including l-cysteine (Cys), N-acetyl-l-cysteine (NAC), and dithiothreitol (DTT), on bovine sperm DNA integrity. The addition of Cys or NAC (0.5, 1, or 5 mM) to the medium caused an increase in DNA damage, which was observed either throughout the sperm head or specifically in the post-acrosome (PA) region. DTT treatment increased DNA damage in both the PA and acrosomal regions, and it induced progressive sperm head lysis from the acrosome to the entire head. These treatments caused sperm DNA-damage rates that exceeded the normal range, suggesting that thiol groups enhance the detection of DNA damage. The effects of these thiols on IVF outcomes were further examined. Short-term exposure to 1 mM Cys before insemination improved cleavage rates, whereas prolonged exposure at concentrations ≥ 1 mM during insemination reduced cleavage rates. NAC supplementation had no significant effects. A mild negative correlation was observed between TUNEL-positive sperm rates and both cleavage and blastocyst formation rates following short-term treatment with 0.5 mM Cys. These findings suggest that thiol supplementation, when optimally dosed and timed, may enhance the sensitivity of DNA-damage detection rather than induce additional damage.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"618-624"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Germ cells are hidden contributors to sex differences in vertebrate lifespan. 生殖细胞是脊椎动物寿命性别差异的隐藏因素。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-05-10 DOI: 10.1262/jrd.2026-044

Kota Abe, Tohru Ishitani

{"title":"Germ cells are hidden contributors to sex differences in vertebrate lifespan.","authors":"Kota Abe, Tohru Ishitani","doi":"10.1262/jrd.2026-044","DOIUrl":"10.1262/jrd.2026-044","url":null,"abstract":"Aging is a complex biological process whose regulatory mechanisms remain incompletely understood. Accumulating evidence indicates that germ cells play pivotal roles in the systemic regulation of aging. The link between germ cells and somatic aging was first established in invertebrate models, where germ cells positively regulate the rate of organismal aging. However, whether and how this relationship operates in vertebrates has remained unresolved for nearly a quarter of a century. Recently, using the short-lived vertebrate model Nothobranchius furzeri, we demonstrated that germ cells exert sex-dependent effects on somatic aging. In males, germ cell ablation improved health span and extended lifespan, accompanied by enhanced vitamin D signaling. In contrast, germ cell removal in females shortened lifespan, associated with increased IGF-1 signaling and reduced estrogen signaling. These findings suggest a vertebrate-specific mechanistic link between germ cells and somatic tissues mediated by sex-specific endocrine signaling. Such a mechanism may contribute to sexual dimorphism in reproductive strategies and potentially underlie the female longevity advantage observed across many species. In this review, we synthesize current evidence for germ cell-mediated regulation of systemic aging, propose that germ cells act as central endocrine orchestrators coordinating reproduction and lifespan, and discuss their potential contribution to sex differences in lifespan.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"597-605"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147868802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GM-CSF increases glucose uptake and mitochondrial activity and reduces reactive oxygen species levels in mouse cumulus-oocyte complexes during oocyte in vitro maturation. 在卵母细胞体外成熟过程中，GM-CSF增加小鼠卵丘-卵母细胞复合物的葡萄糖摄取和线粒体活性，降低活性氧水平。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-05-14 DOI: 10.1262/jrd.2025-045

Anmol Saini, Hannah E Lyons, Nicole O McPherson, Mark B Nottle

{"title":"GM-CSF increases glucose uptake and mitochondrial activity and reduces reactive oxygen species levels in mouse cumulus-oocyte complexes during oocyte in vitro maturation.","authors":"Anmol Saini, Hannah E Lyons, Nicole O McPherson, Mark B Nottle","doi":"10.1262/jrd.2025-045","DOIUrl":"10.1262/jrd.2025-045","url":null,"abstract":"We have previously reported that the addition of granulocyte macrophage-colony stimulating factor (GM-CSF) during oocyte in vitro maturation (IVM) can increase embryo development in mice. The present study examined glucose uptake, mitochondrial activity, reactive oxygen species (ROS) levels, and the expression of key genes associated with these pathways in cumulus-oocyte complexes (COCs). The addition of 10 ng/ml GM-CSF during IVM increased glucose uptake at 15 min (3.45 vs. 3.48, P < 0.001) compared control. However, at 30 min (3.45 vs. 3.44, P < 0.05) and 60 min (3.45 vs. 3.46, P < 0.01), glucose levels were higher in the control. GM-CSF also increased mitochondrial membrane potential (MMP) in the outer area of COCs compared with the control at the end of IVM (1.92 vs. 2.42, P < 0.01), while no differences were observed in the intermediate and perinuclear areas. GM-CSF reduced MMP across all three regions in denuded oocytes compared with control (outer area 3.64 vs. 3.32, P < 0.05; intermediate 2.05 vs. 1.63, P < 0.01; perinuclear 1.59 vs. 1.28, P < 0.01). GM-CSF reduced ROS levels compared with the control at the end of IVM (6367.7 vs. 3935.8, P < 0.01). ROS levels were also reduced in denuded oocytes in the GM-CSF group with the control group (14870.0 vs. 7117.9, P < 0.001). GM-CSF increased the expression of key genes associated with mitochondrial function and ROS production in COCs at the end of IVM. Together, our findings suggest that GM-CSF increases glucose uptake in the cumulus cells which increases the supply of energy substrates to the oocyte increasing mitochondrial activity while reducing ROS levels.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"671-679"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147930763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of post-insemination GnRH analogue administration on pregnancy rates in repeat-breeder cows: Comparison of dosage and frequency. 授精后给药GnRH类似物对重复种牛妊娠率的影响：剂量和频率的比较。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-05-01 DOI: 10.1262/jrd.2026-043

Fernando López-Gatius, Mónica Pando, Irina Garcia-Ispierto

{"title":"Effect of post-insemination GnRH analogue administration on pregnancy rates in repeat-breeder cows: Comparison of dosage and frequency.","authors":"Fernando López-Gatius, Mónica Pando, Irina Garcia-Ispierto","doi":"10.1262/jrd.2026-043","DOIUrl":"10.1262/jrd.2026-043","url":null,"abstract":"Repeat-breeder cows (defined as having > 3 inseminations) significantly impact dairy profitability worldwide. This study evaluated the clinical efficacy of two elevated doses of the GnRH analogue, dephereline, administered during the early luteal phase (5-7 days post-insemination) to improve fertility in high-producing repeat-breeder cows (n = 551). Cows were assigned to a control group (n = 82), a 2 DEPH group (two single doses 24 h apart; n = 235), or a 2.5 DEPH group (a 2.5-fold single dose; n = 234). Pregnancy was recorded in 232 (42.1%) cows: 32 (39%), 100 (42.6%), and 100 (42.7%), in the control, 2DEPH and 2.5DEPH, respectively. Compared to the control, both treatment protocols significantly increased the probability of additional corpus luteum formation in pregnant cows (OR: 5.4 and 8.6, respectively) and reduced pregnancy loss (OR: 0.3 and 0.4, respectively). These findings suggest that both elevated dephereline regimens effectively enhance luteal support and pregnancy maintenance, offering practical therapeutic options for clinical management.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"727-731"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Holding temperature to transport immature bovine oocytes in collection media critically affects their developmental competence. 在收集介质中保持温度运输未成熟牛卵母细胞对其发育能力有重要影响。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-04-27 DOI: 10.1262/jrd.2026-042

Kenichiro Sakaguchi, Thomas Chia-Tang Ho, Moeka Saito, Takashi Tanida, Yojiro Yanagawa, Seiji Katagiri, Kohei Kawano

{"title":"Holding temperature to transport immature bovine oocytes in collection media critically affects their developmental competence.","authors":"Kenichiro Sakaguchi, Thomas Chia-Tang Ho, Moeka Saito, Takashi Tanida, Yojiro Yanagawa, Seiji Katagiri, Kohei Kawano","doi":"10.1262/jrd.2026-042","DOIUrl":"10.1262/jrd.2026-042","url":null,"abstract":"We investigated the holding condition in collection media to establish transportation system of immature bovine cumulus-oocyte complexes (COCs). We examined holding temperature (38°C or 20°C), basal media (Lactated Ringer's solution and TCM199) and supplementation of hypoxanthine, an inhibitor of meiotic resumption. As a result, no blastocyst was obtained from COCs held at 38°C for 6 h or longer, whereas blastocysts were obtained even after 24 h holding in all experimental groups held at 20°C. The blastulation rate was maintained (42%) in TCM199 with hypoxanthine group even after 12 h holding, while reduction of the blastulation rates were observed in all other groups (P < 0.05). After 24 h of storage, Lactated Ringer's solution without hypoxanthine showed the highest blastulation rate (40%). In conclusion, holding temperature of oocytes critically affects the developmental competence of oocytes, and oocytes held at 20°C maintain their developmental competence for at least 12 h.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"723-726"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147774610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture medium modulates nucleolar architecture and disrupts ribosomal RNA levels in preimplantation mouse embryos. 培养基调节着床前小鼠胚胎的核仁结构并破坏核糖体RNA水平。

IF 2.1 4区生物学

Journal of Reproduction and Development Pub Date : 2026-08-01 Epub Date: 2026-06-03 DOI: 10.1262/jrd.2026-004

Md Wasim Bari, Jannatul Ferdous Jharna, Norermi Firzana Alfian, Shiori Ishiyama, Kazuki Mochizuki, Satoshi Kishigami

{"title":"Culture medium modulates nucleolar architecture and disrupts ribosomal RNA levels in preimplantation mouse embryos.","authors":"Md Wasim Bari, Jannatul Ferdous Jharna, Norermi Firzana Alfian, Shiori Ishiyama, Kazuki Mochizuki, Satoshi Kishigami","doi":"10.1262/jrd.2026-004","DOIUrl":"10.1262/jrd.2026-004","url":null,"abstract":"Embryonic culture media support preimplantation development in vitro, yet their broader effects on the molecular organization have not been fully characterized. In mammalian embryos, the nucleolus is a prominent and highly dynamic organelle that orchestrates ribosome biogenesis through RNA polymerase I-dependent ribosomal DNA (rDNA) transcription. However, the effect of culture conditions on nucleolar organization and rRNA dynamics during early development remains poorly understood. In this study, we investigated the effects of two compositionally distinct culture media on the nucleolar architecture and rRNA levels in mouse preimplantation embryos cultured in vitro. In vitro fertilized embryos were cultured in either Chatot-Ziomek-Bavister (CZB) or α-minimal essential medium (α-MEM). The nucleolar morphology was assessed by analyzing the localization of the nucleolar protein NOPP140 and rRNA levels were quantified by immunostaining the embryos with the Y10b antibody clone. Quantitative analyses showed that embryos cultured in α-MEM exhibited a significantly higher proportion of single and enlarged nucleoli per blastomere than those cultured in CZB (P < 0.05). However, α-MEM cultured embryos displayed a significant reduction in Y10b signal intensity relative to CZB-cultured embryos (P < 0.05). Although developmental rates were similar between the culture conditions, embryos cultured in α-MEM displayed altered cell lineage allocation compared to those cultured in CZB. Together, these findings indicate that culture medium regulates nucleolar organization and rRNA abundance in mouse preimplantation embryos, with nucleolar plasticity serving as a sensitive molecular indicator of in vitro culture-dependent developmental effects.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":" ","pages":"680-690"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13441537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148156972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0