Journal of Reproduction and Development最新文献_第5页

High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm 高浓度牛血清白蛋白可提高冷藏大鼠精子的受精能力

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-03-01 DOI: 10.1262/jrd.2023-085

Katsuma YAMAGA, Satohiro NAKAO, Nobuyuki MIKODA, Jorge Mario SZTEIN, Naomi NAKAGATA, Toru TAKEO

{"title":"High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm","authors":"Katsuma YAMAGA, Satohiro NAKAO, Nobuyuki MIKODA, Jorge Mario SZTEIN, Naomi NAKAGATA, Toru TAKEO","doi":"10.1262/jrd.2023-085","DOIUrl":"https://doi.org/10.1262/jrd.2023-085","url":null,"abstract":"Cold transport of the cauda epididymides is a useful technique for shipping laboratory rat sperm. Cold transport of rat sperm avoids potential risks of microbiological infection, animal escape or death, and animal welfare issues. Previously, we reported that a cold-storage solution containing dimethyl sulfoxide and quercetin maintained the fertility of cold-stored rat sperm. However, cold-stored rat sperm exhibited a decreased fertilization rate after 24-h storage. To recover the fertility of cold-stored sperm, we focused on the effects of bovine serum albumin (BSA), a cholesterol acceptor that induces sperm capacitation. We sought to determine the optimal concentration of BSA in fertilization medium based on the fertility of cold-stored rat sperm. High concentrations of BSA (40 mg/ml) enhanced the fertilization rate of cold-stored rat sperm and maintained sperm fertility for 144 h. Embryos derived from cold-stored and BSA-treated sperm normally developed into pups after embryo transfer. In summary, high BSA concentrations enhanced the fertility of cold-stored rat sperm and prolonged the storage period to 144 h, thereby expanding the transportable region for genetically engineered rats.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-085/figure/advpub_2023-085.png\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140008653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes 排卵和授精之间的时间间隔决定大鼠受精卵细胞的质量

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-02-26 DOI: 10.1262/jrd.2023-067

Naomi NAKAGATA, Satohiro NAKAO, Nobuyuki MIKODA, Katsuma YAMAGA, Toru TAKEO

{"title":"Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes","authors":"Naomi NAKAGATA, Satohiro NAKAO, Nobuyuki MIKODA, Katsuma YAMAGA, Toru TAKEO","doi":"10.1262/jrd.2023-067","DOIUrl":"https://doi.org/10.1262/jrd.2023-067","url":null,"abstract":"Genetically modified rats are valuable models in human disease research. We recently developed an improved system for rat sperm cryopreservation and in vitro fertilization (IVF) that facilitates the efficient production and preservation of genetically modified rats. In the IVF procedure performed using frozen–thawed rat sperm, the IVF schedule is fixed to ensure timely hormone administration and oocyte collection. To enhance the flexibility of the IVF schedule, possible periods of postovulated rat oocytes with normal fertility and developmental abilities should be determined. Therefore, in this study, we examined the fertilization and developmental ability of incubated oocytes 1–13 h after oocyte collection at 9:00 AM. The fertilization rate decreased 7 h after oocyte collection, and abnormally fertilized oocytes appeared 10 h after oocyte collection. The developmental rate also decreased 7 h after oocyte collection; however, live pups were obtained from oocytes 12 h after oocyte collection. In summary, ovulated rat oocytes exhibited a high developmental ability after IVF for up to 4 h after oocyte collection.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-067/figure/advpub_2023-067.png\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139950578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of chemokines in regulating luteal and uterine functions in pregnant cows 趋化因子在调节妊娠母牛黄体和子宫功能中的作用

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-02-25 DOI: 10.1262/jrd.2023-100

Ryosuke SAKUMOTO

引用次数: 0

Capturing temperature changes on the ocular surface along with estrus and ovulation using infrared thermography in Japanese Black cows. 利用红外热像仪捕捉日本黑牛眼表温度随发情和排卵的变化。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-02-19 Epub Date: 2023-11-23 DOI: 10.1262/jrd.2022-116

Riho Ozaki, Seiji Inoue, Yuki Yorozui, Rei Ichikawa, Naoki Yamada, Seiya Higashi, Shuichi Matsuyama, Hiroko Tsukamura, Satoshi Ohkura, Yoshihisa Uenoyama, Yasuhiro Morita

{"title":"Capturing temperature changes on the ocular surface along with estrus and ovulation using infrared thermography in Japanese Black cows.","authors":"Riho Ozaki, Seiji Inoue, Yuki Yorozui, Rei Ichikawa, Naoki Yamada, Seiya Higashi, Shuichi Matsuyama, Hiroko Tsukamura, Satoshi Ohkura, Yoshihisa Uenoyama, Yasuhiro Morita","doi":"10.1262/jrd.2022-116","DOIUrl":"10.1262/jrd.2022-116","url":null,"abstract":"Pre-ovulatory follicles are cooler than the neighboring reproductive organs in cows. Thus, measuring the temperature of reproductive organs could be a useful method for predicting estrus and ovulation in cows, and the establishment of a non-invasive technique is required. In this study, we used infrared thermography (IRT) to measure ocular surface temperature as a potential surrogate for reproductive organ temperature. Five Japanese Black cows with synchronized estrus were subjected to temperature measurements in five regions of the ocular surface, including the nasal conjunctiva, nasal limbus, center cornea, temporal limbus, and temporal conjunctiva, twice a day (0800 h and 1600 h) during the experimental period. The temperatures in the five regions significantly declined in cows from estrus to ovulation. To the best of our knowledge, this study is the first to use IRT to show a temperature decrease in the ocular surface along with estrus to ovulation in Japanese Black cows.","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10902635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138440895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PABPN1L is required for maternal mRNA degradation after meiosis resumption. 减数分裂恢复后母体mRNA降解需要PABPN1L。

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-02-19 Epub Date: 2023-12-07 DOI: 10.1262/jrd.2023-077

Chihiro Emori, Mayo Kodani, Ferheen Abbasi, Masashi Mori, Masahito Ikawa

引用次数: 0

Lipid droplets synthesized during luteinization are degraded after pregnancy 黄体化过程中合成的脂滴在怀孕后会降解

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-02-04 DOI: 10.1262/jrd.2023-095

Junichiro MITSUI, Megumi IBAYASHI, Ryutaro AIZAWA, Tomonori ISHIKAWA, Naoyuki MIYASAKA, Satoshi TSUKAMOTO

{"title":"Lipid droplets synthesized during luteinization are degraded after pregnancy","authors":"Junichiro MITSUI, Megumi IBAYASHI, Ryutaro AIZAWA, Tomonori ISHIKAWA, Naoyuki MIYASAKA, Satoshi TSUKAMOTO","doi":"10.1262/jrd.2023-095","DOIUrl":"https://doi.org/10.1262/jrd.2023-095","url":null,"abstract":"After pregnancy, the corpus luteum (CL) functions as a transient endocrine gland that produces progesterone, which is necessary to maintain pregnancy. To maintain constant progesterone production, CLs are enriched in lipids as its precursors. Lipid droplets (LDs) are organelles that originate from the endoplasmic reticulum and store neutral lipids such as triacylglycerols and cholesteryl esters. The size and number of LDs in a cell are regulated by LD-associated proteins that coat their surface. LD degradation is regulated by either neutral lipid hydrolases (lipolysis), selective autophagic mechanism (lipophagy), or both. Mammalian CLs are long known to be enriched in LDs, but LDs are rapidly depleted after pregnancy and reappear near the time of delivery. In this present study, we hypothesized that LDs synthesized by luteinization are massively degraded after pregnancy. Using mCherry-HPos mice, in which LD synthesis can be visualized in vivo, we found that LD synthesis, which was activated during luteal development, was suppressed after implantation. In CLs, LD synthesis remained low during pregnancy, but was reactivated before and after delivery. These changes in LDs were confirmed using electron microscopy and immunostaining. Furthermore, LD degradation was mediated by lipolysis rather than lipophagy. In summary, our findings indicate that luteinization-induced LD synthesis is suppressed after pregnancy onset and that CLs are lipid-poor during pregnancy because LDs stored during luteal development are extensively degraded by lipolysis.\u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-095/figure/advpub_2023-095.jpg\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139678088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The fertility of dairy heifers and cows is not influenced by the follicular wave of the ovulatory follicle 乳用小母牛和奶牛的繁殖力不受排卵卵泡的卵泡波影响

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-01-20 DOI: 10.1262/jrd.2023-084

Javad MOHAMMADI, Mehdi AZARI, Mojtaba KAFI

{"title":"The fertility of dairy heifers and cows is not influenced by the follicular wave of the ovulatory follicle","authors":"Javad MOHAMMADI, Mehdi AZARI, Mojtaba KAFI","doi":"10.1262/jrd.2023-084","DOIUrl":"https://doi.org/10.1262/jrd.2023-084","url":null,"abstract":"Two studies were conducted to evaluate the effects of the follicular wave on ovarian function and fertility in dairy heifers and lactating cows. In study 1, the estrous cycle of the selected Holstein heifers was initially synchronized using two intra-muscular prostaglandin F2α (PGF2α) administrations 11 days apart. Heifers in group FFW (n = 14) received an intra-muscular 500 μg PGF2α administration on day 7 after detecting standing estrus, while Heifers in group SFW (n = 14) were administered PGF2α 13 days after detecting standing estrus. The pregnancy rates of FFW (n = 98) and SFW (n = 100) heifers were also determined 35–37 days after artificial insemination (AI). In Study 2, healthy Holstein lactating cows (n = 28) were randomly assigned to either the FFW (n = 14) or SFW (n = 14) groups. The estrous cycles of the cows were presynchronized using two intra-muscular administrations of PGF2α given 14 days apart. Then, the emergences of the follicular waves were induced using an Ovsynch protocol. The pregnancy rate of FFW (n = 99) versus SFW (n = 98) cows was also determined 35–37 days after AI. The ovulatory follicle and corpus luteum (CL) resulting from the ovulatory follicle of FFW were larger than those of the dominant follicle and the CL of SFW in dairy heifers and lactating cows. However, the pregnancy rate did not differ between the FFW and SFW groups in heifers and lactating cows 35–37 days after AI. In conclusion, although the characteristics of the ovulatory follicles in FFW versus SFW animals differed, the follicular wave in dairy heifers or lactating cows did not affect fertility.\u0000","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139501064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum-free spontaneously immortalized bovine oviduct epithelial cell conditioned medium promotes the early development of bovine in vitro fertilized embryos 无血清自发永生牛输卵管上皮细胞条件培养基促进牛体外受精胚胎的早期发育

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-01-19 DOI: 10.1262/jrd.2023-031

Norikazu MIYASHITA, Satoshi AKAGI, Tamas SOMFAI, Yuji HIRAO

{"title":"Serum-free spontaneously immortalized bovine oviduct epithelial cell conditioned medium promotes the early development of bovine in vitro fertilized embryos","authors":"Norikazu MIYASHITA, Satoshi AKAGI, Tamas SOMFAI, Yuji HIRAO","doi":"10.1262/jrd.2023-031","DOIUrl":"https://doi.org/10.1262/jrd.2023-031","url":null,"abstract":"Embryonic transfer of bovine blastocysts produced using in vitro fertilization (IVF) is widely used, although the challenge of compromised conception rates remains. Using bovine oviduct epithelial cells (BOEC) to improve embryo culture conditions has attracted attention, particularly since the recent discovery of extracellular vesicles from BOEC. The selection of embryos for transfer has also been the subject of various studies, and a set of evaluation criteria to predict pregnancy success has been suggested, in which the embryos are judged by their kinetics and morphology at the early stages. In the present study, we established a spontaneously immortalized BOEC line (SI-BOEC) and examined the effects of conditioned medium on IVF embryos, focusing on the results of the recommended criteria. A modified KSOM (mKSOM) was used to prepare conditioned media. Presumptive zygotes were cultured in mKSOM (control), SI-BOEC-conditioned medium, mKSOM supplemented with sediment (pellet) collected after the ultracentrifugation of the conditioned medium (mKSOM/sediment), and the supernatant. A significantly higher percentage of embryos satisfied the recommended criteria when grown in the conditioned medium than in the mKSOM. A higher proportion of embryos developed into blastocysts after achieving the four criteria. A similar tendency was observed when grown in mKSOM/sediment compared to mKSOM; however, this was not observed in the supernatant. Vesicles with a size similar to that of exosomes were observed in the sediment. In conclusion, the culture medium conditioned by SI-BOEC promoted the production of bovine blastocysts that satisfied the four evaluation criteria recommended for embryo selection. \u0000\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jrd/advpub/0/advpub_2023-031/figure/advpub_2023-031.png\"/>\u0000Graphical Abstract Fullsize Image","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139497197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of dystrophin-locus-derived testis-specific protein: a testis-specific gene within the intronic region of the rat dystrophin gene 睾丸特异性蛋白的鉴定和表征：大鼠睾丸营养不良蛋白基因内含子区的睾丸特异性基因

IF 1.8 4区生物学

Journal of Reproduction and Development Pub Date : 2024-01-19 DOI: 10.1262/jrd.2023-073

Keitaro YAMANOUCHI, Shizuka KATO, Yukie TANAKA, Masanari IKEDA, Yukina OSHIMO, Takanori SHIGA, Kei HATAMOTO, James CHAMBERS, Takuya IMAMURA, Ryuji HIRAMATSU, Kazuyuki UCHIDA, Fuko MATSUDA, Takashi MATSUWAKI, Tetsuya KOHSAKA

{"title":"Identification and characterization of dystrophin-locus-derived testis-specific protein: a testis-specific gene within the intronic region of the rat dystrophin gene","authors":"Keitaro YAMANOUCHI, Shizuka KATO, Yukie TANAKA, Masanari IKEDA, Yukina OSHIMO, Takanori SHIGA, Kei HATAMOTO, James CHAMBERS, Takuya IMAMURA, Ryuji HIRAMATSU, Kazuyuki UCHIDA, Fuko MATSUDA, Takashi MATSUWAKI, Tetsuya KOHSAKA","doi":"10.1262/jrd.2023-073","DOIUrl":"https://doi.org/10.1262/jrd.2023-073","url":null,"abstract":"The mammalian X chromosome exhibits enrichment in genes associated with germ cell development. Previously, we generated a rat model of Becker muscular dystrophy (BMD) characterized by an in-frame mutation in the dystrophin gene, situated on the X chromosome and responsible for encoding a protein crucial for muscle integrity. Male BMD rats are infertile owing to the absence of normal spermatids in the epididymis. Within the seminiferous tubules of BMD rats, elongated spermatids displayed abnormal morphology. To elucidate the cause of infertility, we identified a putative gene containing an open reading frame situated in the intronic region between exons 6 and 7 of the dystrophin gene, specifically deleted in male BMD rats. This identified gene, along with its encoded protein, exhibited specific detection within the testes, exclusively localized in round to elongated spermatids during spermiogenesis. Consequently, we designated the encoded protein as dystrophin-locus-derived testis-specific protein (DTSP). Given the absence of DTSP in the testes of BMD rats, we hypothesized that the loss of DTSP contributes to the infertility observed in male BMD rats.\u0000","PeriodicalId":16942,"journal":{"name":"Journal of Reproduction and Development","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139506384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Lower developmental potential of rat zygotes produced by ooplasmic injection of testicular spermatozoa versus cauda epididymal spermatozoa. 更正：通过卵浆注射睾丸精子与尾状附睾精子产生的大鼠子代的发育潜能较低。

IF 1.9 4区生物学

Journal of Reproduction and Development Pub Date : 2024-01-01 DOI: 10.1262/jrd.2024-030e

Misuzu Ide, Ibuki Saito, Makoto Sanbo, Mito Kanatsu-Shinohara, Takashi Shinohara, Masumi Hirabayashi, Shinichi Hochi

引用次数: 0