Journal of Pest Science最新文献_第3页

Cellular and functional heterogeneity of fall armyworm (Spodoptera frugiperda) midgut: a single-cell RNA sequencing analysis 秋军虫（Spodoptera frugiperda）中肠的细胞和功能异质性：单细胞 RNA 测序分析

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2024-09-16 DOI: 10.1007/s10340-024-01828-4

Surjeet Kumar Arya, Douglas A. Harrison, Subba Reddy Palli

{"title":"Cellular and functional heterogeneity of fall armyworm (Spodoptera frugiperda) midgut: a single-cell RNA sequencing analysis","authors":"Surjeet Kumar Arya, Douglas A. Harrison, Subba Reddy Palli","doi":"10.1007/s10340-024-01828-4","DOIUrl":"https://doi.org/10.1007/s10340-024-01828-4","url":null,"abstract":"Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for studying complex cellular composition and gene expression dynamics of biological systems. In this study, we analyzed the midgut of the fall armyworm (FAW), Spodoptera frugiperda, utilizing scRNA-seq technology. scRNA-seq analysis yielded high-quality sequencing data from two replicates, showcasing robust sequencing integrity, mapping efficiency, and reproducibility. We identified twelve clusters of midgut cells, including enterocytes, enteroblasts, enteroendocrine cells, goblet cells, and stem cells, each with unique marker gene expression indicative of their specialized functions. Further analysis revealed intricate gene expression profiles and enriched biological pathways associated with each cell type, shedding light on the molecular mechanisms underlying midgut function. Additionally, lineage trajectory analysis identified the differentiation pathways of midgut cell populations, confirming canonical relationships among stem cells, enteroblasts, enterocytes, and goblet cells. Furthermore, we also studied the expression of genes coding for insecticide target sites and metabolizing enzymes in different midgut cell types. Overall, our studies provide a comprehensive understanding of midgut cellular diversity and gene expression dynamics in the FAW, offering valuable information that could be used to develop methods for managing this and other pests.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"19 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142234455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allicin impacts the susceptibility of Spodoptera exigua to nucleopolyhedrovirus 大蒜素影响旋毛虫对核型多角体病毒的易感性

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2024-09-12 DOI: 10.1007/s10340-024-01830-w

Jin-Yan Wang, Jie-Xian Jiang, Neng-Neng Fan, Hua-Wu Wu, Xiang-Yun Ji, Nian-Feng Wan, You-Ming Hou

{"title":"Allicin impacts the susceptibility of Spodoptera exigua to nucleopolyhedrovirus","authors":"Jin-Yan Wang, Jie-Xian Jiang, Neng-Neng Fan, Hua-Wu Wu, Xiang-Yun Ji, Nian-Feng Wan, You-Ming Hou","doi":"10.1007/s10340-024-01830-w","DOIUrl":"https://doi.org/10.1007/s10340-024-01830-w","url":null,"abstract":"Plant secondary metabolites are crucial in affecting the interactions between insect herbivores and entomoviruses. However, there is limited knowledge regarding the impact of such metabolites on the susceptibility of insect herbivores to entomoviruses. In this study, we adopted the allicin, caterpillars (Spodoptera exigua) and nucleopolyhedrovirus (SeMNPV) as a system, and found that allicin significantly increased the mortality of S. exigua larvae infected with SeMNPV by 36.03–59.45% when infected with the virus at a concentration of 2.12 × 103 OB·mL−1. Furthermore, NPV-infected larvae together treated with allicin inhibited the growth and development of larvae, comparing to individual NPV-infected larvae. Notably, we observed a significant enrichment of differentially expressed genes involved in the cytochrome P450-mediated metabolism pathway between the NPV-infected and allicin combined with NPV-treated groups. The silencing of CYP340AA1 through RNA interference significantly increased the mortality of larvae infected with SeMNPV. This investigation indicates that allicin might be a potential candidate for improving the performance of the NPV against insect herbivores and identifies that CYP340AA1 gene is important in this process.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"5 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking the geographical distribution of the Asian citrus psyllid Diaphorina citri throughout China using mitogenomes and endosymbionts 利用有丝分裂基因组和内生共生体追踪亚洲柑橘木虱在中国各地的地理分布情况

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2024-09-11 DOI: 10.1007/s10340-024-01834-6

Fengnian Wu, Zehan Dai, Min Shi, Jianjian Huang, Hui Zhu, Yuzhong Zheng, Zikai Chen, Xiuhong Li, Xiaoling Deng, Eduardo G. P. Fox

{"title":"Tracking the geographical distribution of the Asian citrus psyllid Diaphorina citri throughout China using mitogenomes and endosymbionts","authors":"Fengnian Wu, Zehan Dai, Min Shi, Jianjian Huang, Hui Zhu, Yuzhong Zheng, Zikai Chen, Xiuhong Li, Xiaoling Deng, Eduardo G. P. Fox","doi":"10.1007/s10340-024-01834-6","DOIUrl":"https://doi.org/10.1007/s10340-024-01834-6","url":null,"abstract":"Diaphorina citri is the vector of “Candidatus Liberibacter asiaticus” (CLas), a bacterium associated with the citrus disease known as Huanglongbing (HLB). Previous mitochondrial genome (i.e. mitogenome) population analyses revealed the prevalence of two major mitochondrial groups (MGs) of D. citri in China, separated by elevation gradients. We assessed the population diversity of D. citri from 54 major citrus-producing areas within 11 provinces/regions of China. Additionally, endosymbiont genomes were assembled for “Ca. Carsonella ruddii” (CaCr) and “Ca. Profftella armatura” (CaPa) from next-generation sequencing of 31 new Chinese samples. Most of the D. citri diversity came from single nucleotide polymorphisms (SNPs) within five mitochondrial genes: nad3, cox2, rrnL, cob, and atp6. Nine SNPs clustered the analyzed D. citri mitogenomes into three major MGs comprising seven subgroups. Independent phylogenetic trees were generated for the endosymbionts CaCr and CaPa, and a CaPa plasmid, supporting the patterns obtained for D. citri mitogenomes while adding complexity layers. Genomic data from CaCr, CaPa, along with the plasmids from CaPa contribute to the genetic diversity—consisting of 68 SNPs and two genomic gaps—reproducing phylogenetic structures outlined by previous mitochondrial genomic studies. Additionally, both the mitogenomes and the endosymbiont genomes revealed subgroups within the original MG clusters, based on further 154 SNPs and 17 gaps. Thus, the combined genomic approach reveals further aspects about population diversity and natural history of this invasive species. Further understanding of D. citri and its endosymbionts can, therefore, aid D. citri HLB management protocols and help forecast territorial expansion events.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"5 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transboundary migration of Spodoptera frugiperda between China and the South-Southeast Asian region 中国与东南亚地区之间的鞘翅目蚜虫跨境迁徙

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2024-09-05 DOI: 10.1007/s10340-024-01833-7

Yifei Song, Haowen Zhang, Kongming Wu

{"title":"Transboundary migration of Spodoptera frugiperda between China and the South-Southeast Asian region","authors":"Yifei Song, Haowen Zhang, Kongming Wu","doi":"10.1007/s10340-024-01833-7","DOIUrl":"https://doi.org/10.1007/s10340-024-01833-7","url":null,"abstract":"Since 2018, increased infestation by the fall armyworm Spodoptera frugiperda (FAW), an invasive pest worldwide, has negatively affected Asian crop production. Seasonal migratory activities drive regional outbreaks of this pest, but it remains unclear whether there is direct transboundary movement between East Asian and South Asian regions. From 2019 to 2023, FAW moth movements were monitored in Ruili of Yunnan Province, a city at the border area of China and Myanmar which is located in the insect migratory route between China and South Asian countries such as India and Bangladesh. The results showed that there was regular seasonal migratory activity of the pest, which could be divided into spring–summer (April–June) and autumn (October) peak migration periods. Further analysis using trajectory simulation model indicated that the FAW moths in spring–summer migration mostly come from Myanmar, the northeastern states of India and Bangladesh, and returns to the three countries in autumn from Southwest China. Our study clarifies the regional migration pattern of the FAW moth in China and South-Southeast Asia, providing a theoretical basis for constructing a regional early warning and management systems of this pest.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"42 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142138174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring multigene families of odorant binding proteins and cytochrome P450 monooxygenases in the stink bug pest complex through comparative transcriptomics 通过比较转录组学探索蝽害虫复合体中气味结合蛋白和细胞色素 P450 单氧化酶的多基因家族

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2024-09-03 DOI: 10.1007/s10340-024-01831-9

Andrea Belén Dulbecco, Débora Elizabeth Moriconi, Fernanda Cingolani, Eliana Nieves, Luis Diambra, Nicolás Pedrini

{"title":"Exploring multigene families of odorant binding proteins and cytochrome P450 monooxygenases in the stink bug pest complex through comparative transcriptomics","authors":"Andrea Belén Dulbecco, Débora Elizabeth Moriconi, Fernanda Cingolani, Eliana Nieves, Luis Diambra, Nicolás Pedrini","doi":"10.1007/s10340-024-01831-9","DOIUrl":"https://doi.org/10.1007/s10340-024-01831-9","url":null,"abstract":"The stink bugs Edessa meditabunda, Piezodorus guildinii, and Diceraeus furcatus (Hemiptera: Pentatomidae) are major pests in the Argentinean core area of soybean production. A detailed molecular genetics comprehension of how these insects perceive odorants and respond to semiochemicals and how they detoxify chemical pesticides and plant compounds are essential to improve their management strategies. We first assembled and compared the transcriptomes from E. meditabunda, P. guildinii, and D. furcatus. Regarding sequence similarity, P. guildinii and D. furcatus are closer to each other than E. meditabunda. Then, we characterized the multigene families of odorant binding proteins (OBPs) and cytochrome P450 monooxygenases (CYP). A total of 29, 38, and 39 unigenes encoding for OBP were obtained in E. meditabunda, P. guildinii, and D. furcatus, respectively, divided into classical OBPs and plus-C OBPs. A total of 72, 63, and 76 unigenes encoding for CYP were found in E. meditabunda, P. guildinii, and D. furcatus, respectively, which were further classified into 24 families and 47 subfamilies. On the other hand, we performed for the first time RNA interference in vivo by dsRNA injection in E. meditabunda, suggesting that this molecular tool can be exploited in future physiological and functional studies in this species.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"10 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142123711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is polyphagy of a specific cryptic Bemisia tabaci species driving the high whitefly populations on cassava in eastern Africa? 非洲东部木薯上大量的粉虱是由一个特定的隐蔽性烟粉虱物种的多食性造成的吗？

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2024-09-02 DOI: 10.1007/s10340-024-01832-8

Annet Namuddu, Osnat Malka, Susan Seal, Sharon van Brunschot, Richard Kabaalu, Christopher Omongo, Shai Morin, John Colvin

{"title":"Is polyphagy of a specific cryptic Bemisia tabaci species driving the high whitefly populations on cassava in eastern Africa?","authors":"Annet Namuddu, Osnat Malka, Susan Seal, Sharon van Brunschot, Richard Kabaalu, Christopher Omongo, Shai Morin, John Colvin","doi":"10.1007/s10340-024-01832-8","DOIUrl":"https://doi.org/10.1007/s10340-024-01832-8","url":null,"abstract":"Since the 1990s, the cryptic whitefly (Bemisia tabaci) has been linked to severe viral disease pandemics affecting cassava, a crucial staple crop in eastern Africa. This surge in whitefly populations has also been observed in other crops and uncultivated plants. While previous surveys have connected the increase on cassava to two specific populations, SSA1 and SSA2, the dynamics behind the population growth on other plants remain unclear. Additionally, other B. tabaci species, including EA1, IO, MED, SSA9, and SSA10, have been found on cassava in smaller numbers. This study aimed to identify the host plants that support the growth and development of different B. tabaci in Uganda by collecting fourth-instar nymphs from cassava and 20 other common host plants. Host transfer experiments were conducted to test the ability of seven species (EA1, MEAM1, MED-Africa Silver Leafing (ASL), SSA1-subgroup1, SSA1-Hoslundia, SSA6, and SSA12) to develop on cassava. The identities of the nymphs were determined using partial mitochondrial cytochrome oxidase 1 sequences. Twelve B. tabaci species were identified, including two novel species, based on the 3.5% nucleotide sequence divergence. Cassava was colonised by SSA1-SG1, SSA1-SG2, and SSA2. The most prevalent species were SSA1-SG1, MED-ASL, and SSA13, which were also the most polyphagous, colonising multiple plant species. Several whitefly species colonised specific weeds, such as Aspilia africana and Commelina benghalensis. The polyphagous nature of these species supports continuous habitats and virus reservoirs. Effective management of whitefly populations in eastern Africa requires an integrated approach that considers their polyphagy and the environmental factors sustaining host plants.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"62 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142123709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colonies under dysbiosis benefit from oxalic acid application: the role of landscape and beekeeping practices in microbiota response to treatment 菌群失调的蜂群受益于草酸的施用：景观和养蜂方法在微生物群对处理的反应中的作用

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2024-09-02 DOI: 10.1007/s10340-024-01829-3

June Gorrochategui-Ortega, Marta Muñoz-Colmenero, Egoitz Galartza, Andone Estonba, Iratxe Zarraonaindia

{"title":"Colonies under dysbiosis benefit from oxalic acid application: the role of landscape and beekeeping practices in microbiota response to treatment","authors":"June Gorrochategui-Ortega, Marta Muñoz-Colmenero, Egoitz Galartza, Andone Estonba, Iratxe Zarraonaindia","doi":"10.1007/s10340-024-01829-3","DOIUrl":"https://doi.org/10.1007/s10340-024-01829-3","url":null,"abstract":"The Varroa destructor mite causes severe losses of Apis mellifera colonies, requiring recurring treatments. One such treatment is oxalic acid (OA), considered ecological. However, it is unclear whether OA affects the honey bee gut microbiota or other hive-associated microbiotas. Herein, we studied the effect of three OA treatments (trickling at 2.1% or 4.2%, and sublimation through Varrox®) upon microbial communities associated with workers’ gut, hive bee bread and pupae, sampled from conventionally or ecologically managed colonies under different anthropization levels (located in urban, rural or mountainous landscapes). We hypothesized that treatment with OA would impact the diversity and composition of bacteria and/or eukaryotic communities, and that the effect would be dose-dependent and specific to the beehive niche. Results showed that the microbiomes of apiaries under different anthropization levels and management strategies differed prior to OA application. Neither the bacterial nor the fungal communities of bee bread and pupae shifted due to OA treatment. Independent of the dosage and the application method (trickling or sublimation), OA induced slight compositional changes in the bacterial profiles of honeybee guts. Those changes were stronger the higher the anthropization (in colonies from urban areas under conventional management). OA treatment reduced the relative abundance of several pathogens, such as Nosema ceranae, and decreased the overall bacterial diversity down to values found in less anthropized colonies. Thus, our results suggest that, aside from managing Varroa infestations, OA could have beneficial effects for stressed colonies while not impairing honey bee resilience from a microbial point of view.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"18 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142123708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nematicidal activity of the essential oil from Cinnamomum cassia and (E)-cinnamaldehyde against phytoparasitic nematodes 肉桂精油和(E)-肉桂醛对植物寄生线虫的杀线虫活性

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2024-08-30 DOI: 10.1007/s10340-024-01816-8

T. D’Addabbo, S. Laquale, P. Veronico, P. Avato, M. P. Argentieri

{"title":"Nematicidal activity of the essential oil from Cinnamomum cassia and (E)-cinnamaldehyde against phytoparasitic nematodes","authors":"T. D’Addabbo, S. Laquale, P. Veronico, P. Avato, M. P. Argentieri","doi":"10.1007/s10340-024-01816-8","DOIUrl":"https://doi.org/10.1007/s10340-024-01816-8","url":null,"abstract":"The activity of Cinnamomum cassia essential oil (EO) and (E)-cinnamaldehyde was investigated on the phytoparasitic species Meloidogyne incognita, Globodera rostochiensis, and Xiphinema index. Juveniles (J2) or eggs of M. incognita and G. rostochiensis and mixed-age specimens of X. index were exposed to 12.5–100 µg mL−1 concentrations of the two products. The suppressiveness of soil treatments with 100–800 mg kg−1 soil rates of the C. cassia EO and (E)-cinnamaldehyde to M. incognita and G. rostochiensis was assessed on potted tomato and potato, respectively. A 24-h exposure to a 12.5 µg mL−1 solution of (E)-cinnamaldehyde resulted in more than 68% mortality of M. incognita J2, while a poor mortality occurred at the same concentration of the whole EO. The mortality of G. rostochiensis J2 ranged 39 and 42%, respectively, since after a 4-h exposure to a 12.5 µg mL−1 solution of both products. All the X. index specimens died after a 48- and 8-h exposure to a 100 µg mL−1 solution of the EO and (E)-cinnamaldehyde, respectively. Egg hatch was reduced by more than 90% after exposing the M incognita egg masses or the G. rostochiensis cysts to 800 µg mL−1 concentration of both EO and (E)-cinnamaldehyde for 24 and 96 h, respectively. The infestation of M. incognita and G. rostochiensis on tomato and potato, respectively, was significantly reduced by all soil treatments with both products, though (E)-cinnamaldehyde generally resulted more suppressive than the whole EO to both nematode species. According to these results, C. cassia EO and (E)-cinnamaldehyde could be suggested as a potential source of new environment-friendly nematicides.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"54 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alternative domestic rodent pest management approaches to address the hazardous use of metal phosphides in low- and middle-income countries 在低收入和中等收入国家采用替代性家鼠害虫管理方法解决金属磷化物的危险使用问题

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2024-08-22 DOI: 10.1007/s10340-024-01825-7

Alexander M. Stuart, Jens Jacob, Adedayo Michael Awoniyi, Federico Costa, Luwieke Bosma, Yonas Meheretu, Nyo Me Htwe, Stephanie Williamson, Michael Eddleston, Ambroise Dalecky, Sheila Willis

{"title":"Alternative domestic rodent pest management approaches to address the hazardous use of metal phosphides in low- and middle-income countries","authors":"Alexander M. Stuart, Jens Jacob, Adedayo Michael Awoniyi, Federico Costa, Luwieke Bosma, Yonas Meheretu, Nyo Me Htwe, Stephanie Williamson, Michael Eddleston, Ambroise Dalecky, Sheila Willis","doi":"10.1007/s10340-024-01825-7","DOIUrl":"https://doi.org/10.1007/s10340-024-01825-7","url":null,"abstract":"Metal phosphides, particularly aluminium phosphide (AlP) and zinc phosphide (Zn3P2), are widely used in low- and middle-income countries (LMICs) as rodenticides in urban and domestic environments due to their low cost and high toxicity to rodent pests. However, they are also highly toxic to humans with no antidote available and have been associated with numerous fatal cases of intentional and accidental poisoning. This paper reviews alternatives to metal phosphide use for rodent pest management in urban and domestic environments, highlights case studies of effective alternative approaches, and provides recommendations for research and policy. This review identifies numerous alternative methods available for managing rodent pests in domestic/urban settings that can replace metal phosphides. These include chemical methods, i.e. rodenticides, and non-chemical methods, e.g. rodent-proofing, sanitation and trapping. However, because the majority of chemical rodenticides qualify as highly hazardous pesticides due to acute human health toxicity, environmental toxicity, and/or bioaccumulation, simply selecting substitute chemical rodenticides to replace metal phosphides are likely to replace one set of hazards with others. Thus, careful risk and hazard assessments are needed when considering substituting with other chemicals. Overall, we need to move away from current levels of rodenticide reliance towards more integrated and ecologically based approaches.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"6 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142022049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatile organic compounds (VOCs) of Bacillus velezensis TA-1 exhibit toxic effects against Meloidogyne incognita 枯草芽孢杆菌 TA-1 的挥发性有机化合物 (VOC) 对黑僵菌具有毒性作用

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2024-08-12 DOI: 10.1007/s10340-024-01815-9

Xiaoxue Ji, Miao Fan, Di Wang, Siqi Zhang, Shouan Zhang, Yong Liu, Kang Qiao

{"title":"Volatile organic compounds (VOCs) of Bacillus velezensis TA-1 exhibit toxic effects against Meloidogyne incognita","authors":"Xiaoxue Ji, Miao Fan, Di Wang, Siqi Zhang, Shouan Zhang, Yong Liu, Kang Qiao","doi":"10.1007/s10340-024-01815-9","DOIUrl":"https://doi.org/10.1007/s10340-024-01815-9","url":null,"abstract":"The use of Bacillus volatiles to manage root-knot nematodes (RKNs) is a topic that gained extensive attention because it is safe and environmentally benign. Bacillus velezensis TA-1, isolated from a continuously cropped soil, exhibited strong nematicidal activity against RKNs in vitro and in field conditions. However, it remains poorly understood whether TA-1 volatile organic compounds (VOCs) could be effective against Meloidogyne incognita. In this study, the results of chemotaxis activity test revealed that B. velezensis TA-1 exhibited strong repellent effects on second-stage juveniles (J2s) of M. incognita. In in vitro assays, TA-1 VOCs in a three-compartment Petri dish assay exhibited a mortality rate of 84.1% at 48 h and 92.8% at 72 h to M. incognita J2s. Further evidence revealed that TA-1 VOCs significantly inhibited the gene expression of mpk-1, flp-18 and ord-1 by 61.0%, 44.8% and 54.5%, respectively. Moreover, TA-1 VOCs increased the content of reactive oxygen species (ROS), resulting in severe oxidative stress and death of nematodes. Results from a double-layered greenhouse experiment indicated that the populations of J2s in the soil and in cucumber roots were notably reduced by TA-1 VOCs compared to the untreated control. Furthermore, eleven VOCs were identified from TA-1 by solid-phase microextraction gas chromatography-mass spectrometry (SPME-GC/MS) analysis, of which benzaldehyde, 2-decanol, and 2-undecanone had strong contact nematicidal activities against M. incognita J2s. In addition, benzaldehyde and 2-dodecanone exhibited fumigation effects on M. incognita J2s. Overall, these results demonstrated that TA-1 VOCs exhibited direct contact nematicidal and fumigation activities against RKNs and could be considered as potential biocontrol agents for the control of RKNs.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"46 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141918833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0