Journal of Pest Science最新文献

{"title":"Host switching and successive subculturing drive adaptive responses in Liriomyza sativae","authors":"Yang Gao, Wei He, Ming Zhang, Hai-Nuo Hong, Jin Wang, Ying Zhou, Zeng‑Rong Zhu","doi":"10.1007/s10340-026-02033-1","DOIUrl":"https://doi.org/10.1007/s10340-026-02033-1","url":null,"abstract":"","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"30 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing of the V-ATPase gene via RNA interference impairs fitness and survival of the invasive apiary pest Aethina tumida indicating potential for pest control","authors":"Valeria Bonina, Andrea Becchimanzi, Linda Bianco, Giovanna Cangiulli, Paolo Facella, Carlo Fasano, Paolo Iovieno, Giovanni Federico, Salvatore Arpaia","doi":"10.1007/s10340-026-02034-0","DOIUrl":"https://doi.org/10.1007/s10340-026-02034-0","url":null,"abstract":"The small hive beetle ( <jats:italic>Aethina tumida</jats:italic> ) is an invasive pest of honeybee colonies and represents an increasing threat to apiculture outside its native sub-Saharan African range. Its establishment in Italy since 2014 has raised serious concerns for European beekeeping, particularly due to the limited availability of safe and effective chemical control options. RNA interference (RNAi) represents a promising species-specific alternative; however, oral delivery approaches in <jats:italic>A. tumida</jats:italic> have thus far produced inconsistent outcomes. In this study, we assessed lethal and sublethal effects of double-stranded RNA (dsRNA) targeting vacuolar ATPase subunit A (V-ATPase) in laboratory bioassays, using both ingestion and topical application as delivery routes. Ingestion of V-ATPase dsRNA significantly reduced larval growth and prolonged development, resulting in marked delays in adult emergence. Although overall mortality was low, both delivery methods consistently induced strong reductions in adult fecundity. Molecular analyses confirmed consistent knockdown of V-ATPase transcripts following dsRNA exposure. Importantly, bioinformatic analyses revealed no significant sequence homology between the dsRNA constructs and the <jats:italic>Apis mellifera</jats:italic> genome, and injection assays in honeybees showed no acute adverse effects on survival or feeding behavior. Collectively, these results provide the first consistent evidence of RNAi-induced molecular and phenotypic effects in <jats:italic>A. tumida</jats:italic> and underscore the potential of dsRNA-based bioinsecticides as a targeted and pollinator-safe strategy for managing this invasive pest.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"246 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green traps and (Z)-3-hexenol as novel attractive cues for males Coraebus undatus","authors":"Sergio López, Gregor Belušič, Clàudia Corbella-Martorell, Iria Rodríguez, Elisa Tarantino, Antoni Torrell, Josep M. Riba-Flinch, Carmen Quero","doi":"10.1007/s10340-026-02039-9","DOIUrl":"https://doi.org/10.1007/s10340-026-02039-9","url":null,"abstract":"Monitoring and mass trapping are two relevant strategies within the Integrated Pest Management principles. However, the development of an effective trapping method represents a challenge in which multimodal cues should be taken into account to ensure trapping performance. In the case of the flathead oak borer, <jats:italic>Coraebus undatus</jats:italic> (F.) (Coleoptera: Buprestidae), trapping methods so far mainly rely on sticky or interception purple traps that appear attractive for mated females when placed in the understory. Taking into account the visual behaviour of the species and the kairomone activity of the green leaf volatile compound ( <jats:italic>Z</jats:italic> )-3-hexenol in other Buprestidae, we aimed to test the efficacy of green-coloured traps and ( <jats:italic>Z</jats:italic> )-3-hexenol as lures when placed in the canopy. We first examined the electroantennographic and behavioural response of both sexes to ( <jats:italic>Z</jats:italic> )-3-hexenol, and then evaluated the performance of the combination of unbaited canopy-placed sticky green prism traps or baited with ( <jats:italic>Z</jats:italic> )-3-hexenol. Antennae from virgin adults responded to increasing stimuli of ( <jats:italic>Z</jats:italic> )-3-hexenol following a dose–response pattern, although males exhibited a higher response than females overall. In olfactometric trials, ( <jats:italic>Z</jats:italic> )-3-hexenol evoked a positive chemotactic response in males and females. Sticky green prism traps in the canopy were attractive to males, and this attractiveness was significantly increased when releasing ( <jats:italic>Z</jats:italic> )-3-hexenol. Females were also attracted to green traps, although no significant effect of ( <jats:italic>Z</jats:italic> )-3-hexenol was reported. This study highlights the relevance of combining visual and chemical stimuli for efficiently trapping of <jats:italic>C. undatus</jats:italic> , especially males, representing a key step for better monitoring strategies of the species.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"325 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Susceptibility and attractiveness of ancient wheats to Sitophilus granarius and Rhyzopertha dominica","authors":"Ilaria D’Isita, Onofrio Marco Pistillo, Federica Lo Muzio, Sandra Pati, Antonella Di Palma, Christos G. Athanassiou, Pasquale De Vita, Giacinto Salvatore Germinara","doi":"10.1007/s10340-026-02037-x","DOIUrl":"https://doi.org/10.1007/s10340-026-02037-x","url":null,"abstract":"Ancient wheats are valuable genetic resources and could represent a source of income for farmers. Little is known on interactions between ancient grains and storage insect pests. In the present study, the susceptibility and attractiveness of one emmer (Padre Pio) and one spelt (Benedetto) variety toward <jats:italic>Sitophilus granarius</jats:italic> (L.) and <jats:italic>Rhyzopertha dominica</jats:italic> (F.) were evaluated. Samples of Padre Pio and Benedetto, with glumes or without, were infested with adults of <jats:italic>S. granarius</jats:italic> or <jats:italic>R. dominica</jats:italic> to test their susceptibility. A susceptibility index (SI) was calculated for each variety. Both Padre Pio and Benedetto were resistant to <jats:italic>S. granarius</jats:italic> if hulled and moderately resistant if de-hulled. For <jats:italic>R. dominica,</jats:italic> the same varieties were susceptible if hulled and highly susceptible if de-hulled. For <jats:italic>S. granarius</jats:italic> , the SI value was inversely correlated with protein content. The average development time of <jats:italic>R. dominica</jats:italic> was directly correlated with the kernel hardness. The volatile organic compound (VOC) profiles of hulled varieties tested were characterized by headspace solid-phase microextraction (HD-SPME) and gas chromatography coupled with mass spectrometry (GC–MS). The attractiveness toward <jats:italic>S. granarius</jats:italic> and <jats:italic>R. dominica</jats:italic> adults was evaluated in two-choice pitfall bioassays and Y-tube olfactometer, respectively. Both varieties were attractive to <jats:italic>S. granarius</jats:italic> adults, while odors from both varieties did not induce attraction in <jats:italic>R. dominica</jats:italic> adults. The GC–MS analysis highlighted the presence of a total of 50 VOCs. The possible reasons of differences in susceptibility and attractiveness are discussed.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"143 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of non-host aromatic plants on the survival and olfactory behavior of the African citrus psyllid, Trioza erytreae","authors":"Jaciley Bragança-Costa, Daniela Ruano, Nuno Rodrigues, Alberto Fereres, José Alberto Pereira, Isabel Rodrigues","doi":"10.1007/s10340-026-02032-2","DOIUrl":"https://doi.org/10.1007/s10340-026-02032-2","url":null,"abstract":"<jats:italic>Trioza erytreae</jats:italic> Del Guercio, 1918, commonly known as the African citrus psyllid, is one of the main vectors of Huanglongbing (citrus greening), the most destructive citrus disease worldwide. Currently, there is no effective treatment for HLB; therefore, management efforts primarily focus on controlling the insect vectors. This often involves the use of insecticides, which raises concerns about environmental impact and the development of insecticide resistance. This study evaluates the potential of five aromatic non-host plants commonly found in the Mediterranean basin, which overlap with regions of citrus production ( <jats:italic>Cistus ladanifer</jats:italic> , <jats:italic>Rosmarinus officinalis</jats:italic> , <jats:italic>Foeniculum vulgare</jats:italic> , <jats:italic>Lavandula stoechas</jats:italic> , and <jats:italic>Thymus mastichina</jats:italic> ) to influence the survival and olfactory behavior of <jats:italic>T. erytreae</jats:italic> . Although the psyllids were able to survive for several days on these non-host plants, survival was significantly lower compared to the citrus host ( <jats:italic>Citrus limon</jats:italic> ), with <jats:italic>C. ladanifer</jats:italic> having the most detrimental effect. Olfactory assays revealed that, in females, <jats:italic>C. limon</jats:italic> , <jats:italic>C. ladanifer</jats:italic> , and <jats:italic>L. stoechas</jats:italic> elicited significantly higher probabilities of attraction compared with <jats:italic>R. officinalis</jats:italic> and <jats:italic>T. mastichina</jats:italic> . In males, <jats:italic>C. limon</jats:italic> elicited a significantly higher probability of attraction than <jats:italic>R. officinalis</jats:italic> , <jats:italic>T. mastichina</jats:italic> , and <jats:italic>L. stoechas</jats:italic> . A total of 123 volatile organic compounds (VOCs) were identified across all plant species, of which 48 were found to be important for the olfactory response of <jats:italic>T. erytreae</jats:italic> . Among these, 19 VOCs were positively correlated with psyllid attraction, while 15 showed negative correlations, indicating a potential repellent or disruptive effect. These results suggest that specific VOCs emitted by aromatic non-host plants can modulate host-seeking behavior in <jats:italic>T. erytreae</jats:italic> , highlighting their potential application in the development of semiochemical-based, environmentally sustainable strategies for citrus pest management.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"53 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial scale mismatches between pest and natural enemies challenge conservation biological control in tropical organic agroecosystems","authors":"Danyelle R. Novaes, João G. N. Baraldini, Pedro H. B. Togni","doi":"10.1007/s10340-026-02024-2","DOIUrl":"https://doi.org/10.1007/s10340-026-02024-2","url":null,"abstract":"Conservation biological control (CBC) is a key strategy for sustainable pest management in organic farming; yet, its effectiveness is influenced by landscape structure. This study investigates how landscape structure (composition and configuration) affects the tomato leafminer ( <jats:italic>Phthorimaea absoluta</jats:italic> ) and its natural enemies in organic tomato crops in Brazil. Fieldwork was conducted on 20 farms from 2023 to 2024. Pest and natural enemy populations were sampled using pheromone traps, beat sheets, and sticky traps, and biological control was quantified via egg parasitism and predation rates. Landscape metrics were assessed within varying buffer distances using high-resolution land cover data and spatial models. We showed that <jats:italic>P. absoluta</jats:italic> abundance decreased with increasing natural vegetation cover and increased with agricultural patch connectivity, responding at a broad spatial scale (3.1 km). In contrast, natural enemies responded to finer-scale landscape features (0.8 km for parasitoids and 1.6 km for predators), with abundance linked to natural patch size, richness to natural connectivity, and diversity to natural patch shape. Parasitism was enhanced by natural patch connectivity and fractality but reduced by agricultural connectivity and patch size, while predation remained unaffected. These findings demonstrate a spatial mismatch between pest and enemy responses and highlight the importance of landscape configuration beyond habitat amount in enhancing CBC effectiveness. This research underscores the need for spatially explicit landscape planning that promotes enemy-friendly habitats while limiting pest connectivity, offering actionable insights for the design of agroecological strategies in tropical organic systems.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"9 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing thresholds when yield reductions are hard to determine: A case study with the greenhouse whitefly","authors":"Felipe Borrero-Echeverry, Hugo Fernando Rivera-Trujillo, Nadia Luque-Sanabria, Diego Fernando Sánchez-Vivas, Jenny M. Santos-Holguín, Diego F. Rincon","doi":"10.1007/s10340-026-02040-2","DOIUrl":"https://doi.org/10.1007/s10340-026-02040-2","url":null,"abstract":"","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"1 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mosquito-borne disease among individuals experiencing homelessness in the USA: a literature review","authors":"André B. B. Wilke, Megan D. Hill, Allisandra G. Kummer, Chalmers Vasquez, John-Paul Mutebi, Xi Huo, Han Li, Jing Chen, Shigui Ruan, John C. Beier, Maria Litvinova, Thomas Byrne, Marco Ajelli","doi":"10.1007/s10340-026-02042-0","DOIUrl":"https://doi.org/10.1007/s10340-026-02042-0","url":null,"abstract":"Mosquito-borne diseases are a major public health issue. The USA has experienced mosquito-borne disease outbreaks, including West Nile virus, Zika, dengue, and malaria, highlighting widespread risk across the country. In the USA, exposure to mosquito vectors primarily occurs outdoors. Individuals experiencing unsheltered homelessness are particularly vulnerable to mosquito-borne diseases due to their increased exposure to mosquito vectors, limited access to healthcare, and high prevalence of underlying conditions. This review aims to examine the literature on mosquito-borne disease infections among individuals experiencing homelessness in the USA between 1999 and 2024. Our search strategy used Boolean methods on PubMed, Scopus, and Web of Science to select relevant publications. Two queries were used: the first query focused on individuals experiencing homelessness and mosquito-borne diseases, and the second one focused on epidemiological analyses of mosquito-borne disease outbreaks in the USA. Only studies reporting at least one infection among individuals experiencing homelessness were included. Our search resulted in the identification of 347 studies. After screening them, eight were ultimately included in this review. Half of the studies identified West Nile virus among individuals experiencing homelessness, and the other half reported cases of malaria. These studies show that individuals who spend more time outdoors, including individuals experiencing homelessness, may be at a greater risk of MBDs compared to the general population. However, the full extent of mosquito-borne disease exposure among individuals experiencing homelessness remains unclear. With climate change and globalization, there is a consensus in the literature showing an increasing risk of mosquito-borne disease transmission in the next decades, and we have already started to witness this increasing trend. Moreover, the USA is experiencing a steep increase in the overall nationwide homeless population. Therefore, we argue that developing policies aimed at providing homeless populations with housing during mosquito-borne disease outbreaks could be instrumental in protecting this segment of the population and promoting health equity.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"16 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated evaluation of medicinal plant extracts: phytochemical composition, insecticidal activity, and biochemical impacts on the Angoumois grain moth for sustainable grain protection","authors":"Nessma M. Abdeen, Ahmed M. A. Salman, Shaimaa S. Shoman, Fatma S. Ahmed","doi":"10.1007/s10340-026-02038-w","DOIUrl":"https://doi.org/10.1007/s10340-026-02038-w","url":null,"abstract":"Post-harvest losses from insect pests, such as the Angoumois grain moth ( <jats:italic>Sitotroga cerealella</jats:italic> ), remain a major constraint on global food security, particularly in stored cereal grains. This study evaluated methanolic extracts of four medicinal plants— <jats:italic>Lantana camara</jats:italic> , <jats:italic>Origanum majorana</jats:italic> , <jats:italic>Ocimum basilicum</jats:italic> , and <jats:italic>Ammi visnaga</jats:italic> . The study assessed their phytochemical constituents, GC–MS chemical profiles, antioxidant and insecticidal activities, physiological effects on <jats:italic>S. cerealella</jats:italic> , and protective effects on stored grains, while exploring correlations among these properties. <jats:italic>O. basilicum</jats:italic> and <jats:italic>O. majorana</jats:italic> were richest in phenolics (2.66 and 2.10 mg GA g <jats:sup>−1</jats:sup> ) and flavonoids (3.19–3.39 mg QE g <jats:sup>−1</jats:sup> ), corresponding with strong antioxidant capacity (IC <jats:sub>50</jats:sub> = 75.51 and 80.83 µg mL <jats:sup>−1</jats:sup> ). <jats:italic>L. camara</jats:italic> showed the highest acute toxicity (LC <jats:sub>50</jats:sub> = 3.31 mg g <jats:sup>−1</jats:sup> ), whereas <jats:italic>O. basilicum</jats:italic> and <jats:italic>O. majorana</jats:italic> caused the strongest sublethal effects, suppressing adult emergence by &gt; 80% at LC <jats:sub>25</jats:sub> . Grain protection assays demonstrated that <jats:italic>O. basilicum</jats:italic> provided the best long-term protection at LC <jats:sub>90</jats:sub> . Enzyme assays revealed that <jats:italic>O. basilicum</jats:italic> and <jats:italic>O. majorana</jats:italic> significantly enhanced antioxidant defenses (SOD, GSH), whereas <jats:italic>A. visnaga</jats:italic> reduced detoxification-related enzymes (ALT, ALP). Pearson correlation analyses revealed strong negative associations between phenolic content and grain weight loss (GWL) and insect-damaged grains (IDGs), as well as between antioxidant potential (DPPH-IC <jats:sub>50</jats:sub> ) and insect emergence reduction (ER). These findings demonstrate the multifunctional value of phenolic-rich extracts for sustainable botanical pest control and grain preservation, supporting their potential incorporation into sustainable post-harvest management strategies.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"18 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A plant-free push–pull system using sustained-release electrospun micro-/nanofibers for insect behavioral manipulation","authors":"Chenglong Cui, Xiwei Xia, Zhimin Wang, Ertao Li, Huanhuan Dong, Jiaan Shen, Yujun Sun, Yu Peng, Kebin Li, Jiao Yin","doi":"10.1007/s10340-026-02041-1","DOIUrl":"https://doi.org/10.1007/s10340-026-02041-1","url":null,"abstract":"","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"45 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}