Journal of Pest Science最新文献

YOLO-based detection of blueberry fruit fly (Rhagoletis mendax) 基于yolo的蓝莓果蝇检测

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2026-09-09 DOI: 10.1007/s10340-026-02096-0

Janelle M. MacKeil, Craig B. MacEachern, Patrick J. Hennessy, Travis J. Esau, G. Christopher Cutler

{"title":"YOLO-based detection of blueberry fruit fly (Rhagoletis mendax)","authors":"Janelle M. MacKeil, Craig B. MacEachern, Patrick J. Hennessy, Travis J. Esau, G. Christopher Cutler","doi":"10.1007/s10340-026-02096-0","DOIUrl":"https://doi.org/10.1007/s10340-026-02096-0","url":null,"abstract":"Blueberry fruit fly (<italic>Rhagoletis mendax</italic> Curran) (BFF) is a major pest of wild blueberry production because larval infestation reduces fruit quality and can result in rejected berries under zero-tolerance market standards. Monitoring BFF currently relies on visual inspection of ammonium-baited yellow sticky traps, which is labor-intensive and can delay timely management decisions. This study evaluated the feasibility of automated BFF detection from trap images using a YOLO11-based object detector. Images were collected from 27 traps deployed across nine commercially managed wild blueberry fields in Nova Scotia during the 2025 field season. The resulting dataset comprised 528 annotated trap images containing 2,187 BFF instances. A YOLO11-small model was trained using transfer learning and evaluated on an independent held-out test set. On the test set, the detector achieved a precision of 0.925, recall of 0.864, F1-score of 0.893, mAP@0.5 of 0.926, and mAP@0.5:0.95 of 0.681, with an average inference time of 9.6 ms per image. In addition to standard detection metrics, predicted and manual counts were compared on the held-out test dataset (<italic>n</italic> = 53 images). Count agreement was strong, with slight negative bias (− 0.132), low mean absolute error (0.472), low root mean square error (0.836), and high Pearson correlation (<italic>r </italic>= 0.996; 95% CI: 0.992–0.997). Threshold classification accuracy for the 6-fly action threshold was 1.0. Most remaining errors were associated with adhesive glare, partially visible flies at trap edges, and rare but visually similar non-target Tephritidae. A publicly accessible web application was also developed to demonstrate practical deployment. Together, these results show that YOLO-based detection can provide accurate, rapid interpretation of BFF trap images and represents an important step toward automated monitoring and future electronic trap systems for wild blueberry production.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"395 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149003628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut-associated Chryseobacterium enhances starch-derived carbohydrate availability and improves the fitness of Phthorimaea operculella 肠道相关的黄杆菌提高淀粉源性碳水化合物的有效性，并改善了包壳菌的适应性

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2026-09-09 DOI: 10.1007/s10340-026-02085-3

Mengdi Zhang, Junjie Yan, Ralf Nauen, Subba Reddy Palli, Yulin Gao

{"title":"Gut-associated Chryseobacterium enhances starch-derived carbohydrate availability and improves the fitness of Phthorimaea operculella","authors":"Mengdi Zhang, Junjie Yan, Ralf Nauen, Subba Reddy Palli, Yulin Gao","doi":"10.1007/s10340-026-02085-3","DOIUrl":"https://doi.org/10.1007/s10340-026-02085-3","url":null,"abstract":"Lepidopteran pests which feed on plant structures containing high contents of starch, such as tubers or corn grains, need to digest starch to form absorbable low molecular weight sugars for their nutrition. Here, we investigated a gut-associated <italic>Chryseobacterium</italic> strain (X26) isolated from the gut of <italic>Phthorimaea operculella</italic> larvae to assess its contribution to host carbohydrate availability and fitness. Genome analysis of strain X26 revealed a starch utilization system (Sus)-like locus enriched in carbohydrate-active enzyme genes involved in starch degradation. In vivo reintroduction of strain X26 into antibiotic-treated larvae significantly increased starch-derived oligosaccharide levels by 15-fold and exerted probiotic effects on <italic>P. operculella</italic>, as evidenced by shortened developmental duration, higher pupation rates, and increased adult longevity and fecundity, compared with antibiotic-treated controls. Heat-killed bacteria did not confer these benefits, and supplementation with starch-derived oligosaccharides partially restored host performance, supporting an association between microbial carbohydrate processing and improved host fitness. Functional analyses further identified an <italic>α</italic>-amylase that provides mechanistic support for the starch utilization capacity of strain X26. Together, these findings demonstrate that a gut-associated bacterium enhances carbohydrate availability and improves the fitness of <italic>P. operculella</italic>, highlighting the ecological significance of gut microbial associations for an important agricultural pest.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"23 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149003627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pioneer bark beetle attacks orchestrate multifaceted localized defense responses in Norway spruce 先锋树皮甲虫攻击协调多方面的局部防御反应在挪威云杉

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2026-09-01 DOI: 10.1007/s10340-026-02087-1

Marcelo Ramires,Sigrid Netherer,Martin Schebeck,Karin Hummel,Sarah Schlosser,Ebrahim Razzazi-Fazeli,Reinhard Ertl,Muhammad Ahmad,Ana Espinosa-Ruiz,Esther Carrera,Erwann Arc,Teresa Caballero,Jorge Baños,Maria Ángeles Martínez-Godoy,Thomas Ledermann,Michaela Breuer,Silvio Schüler,Marcela van Loo,Carlos Trujillo-Moya

{"title":"Pioneer bark beetle attacks orchestrate multifaceted localized defense responses in Norway spruce","authors":"Marcelo Ramires,Sigrid Netherer,Martin Schebeck,Karin Hummel,Sarah Schlosser,Ebrahim Razzazi-Fazeli,Reinhard Ertl,Muhammad Ahmad,Ana Espinosa-Ruiz,Esther Carrera,Erwann Arc,Teresa Caballero,Jorge Baños,Maria Ángeles Martínez-Godoy,Thomas Ledermann,Michaela Breuer,Silvio Schüler,Marcela van Loo,Carlos Trujillo-Moya","doi":"10.1007/s10340-026-02087-1","DOIUrl":"https://doi.org/10.1007/s10340-026-02087-1","url":null,"abstract":"Abstract Conifer forests around the globe are increasingly threatened by climate change. In addition to abiotic disturbances such as storm-throw, bark beetles are the most widespread cause of forest dieback, and yet, tree defense reactions and underlying molecular pathways have not been fully characterized. To investigate tree responses and molecular defense mechanisms under natural conditions, we used controlled beetle attacks and multi-omics approaches on 35-year-old clonal Norway spruce trees. Attacks by 3–6 individuals of the spruce bark beetle Ips typographus induced coordinated shifts in phytohormones, differential gene and protein expression, and an accumulation of key secondary compounds at the attack site during seven days of exposure. Differential gene expression of up to 1,900 genes from multiple defense-related pathways, together with increased levels of compounds involved in deterring bark beetle attacks, demonstrates how Norway spruce mounts a complex and multifaceted defense response to beetle attack. The comprehensive atlas of expression patterns enables a better understanding of how trees respond at the onset of bark beetle infestation and establishes a basis for future genome-assisted breeding.Please confirm if the author names are presented accurately and in the correct sequence. Author 14 Given name: [Maria Ángeles] Family name [Martínez-Godoy]. Also, kindly confirm the details in the metadata are correctDetails are correct, email for author 14 has been added","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"23 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148922937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Citrus sinensis essential oil-based microemulsion against Plutella xylostella: development and characterization of insecticidal activity 柑桔精油基微乳液对小菜蛾的杀虫活性研究

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2026-09-01 DOI: 10.1007/s10340-026-02078-2

Jônatas S. de Souza,Tárcio S. Santos,Eliana M. dos Passos,Camila de Souza Varize,Joseane de Jesus Oliveira,Juliana C. Cardoso,Rogéria de Souza Nunes,Eliana B. Souto,Patrícia Severino,Marcelo C. Mendonça

{"title":"Citrus sinensis essential oil-based microemulsion against Plutella xylostella: development and characterization of insecticidal activity","authors":"Jônatas S. de Souza,Tárcio S. Santos,Eliana M. dos Passos,Camila de Souza Varize,Joseane de Jesus Oliveira,Juliana C. Cardoso,Rogéria de Souza Nunes,Eliana B. Souto,Patrícia Severino,Marcelo C. Mendonça","doi":"10.1007/s10340-026-02078-2","DOIUrl":"https://doi.org/10.1007/s10340-026-02078-2","url":null,"abstract":"Abstract Plutella xylostella (Linnaeus, 1758) is a pest that causes significant damage to brassica crops around the world. The use of essential oils has led to promising results in the control of P. xylostella; however, the high volatility of these essential oils has limited their use. Therefore, the inclusion of these compounds in microemulsified systems is an alternative to increase their effectiveness in the field. This study proposes the development of a microemulsion, based on Citrus sinensis (Osbeck, 1757) essential oil, for use against P. xylostella . Gas chromatography analysis confirmed that D-limonene is the most dominant compound in the essential oil (88.63%). The microemulsion showed a hydrodynamic diameter of 26.04 ± 0.57 nm and polydispersity index of 0.270 ± 0.02, confirming its monodispersity. The lethal concentration of the C. sinensis essential oil-loaded microemulsion against P. xylostella was estimated in LC 30 = 52.23 mg/mL and LC 50 = 107.79 mg/mL (via contact) and LC 30 = 48.92 mg/mL and LC 50 = 207.29 mg/mL (via ingestion). Furthermore, it reduced larval and pupal viability of P. xylostella and promoted less oviposition of P. xylostella adults than the free essential oil. Our study confirms the insecticidal potential of C. sinensis essential oil-composed microemulsion against P. xylostella and may be proposed as a sustainable alternative for the control of this pest.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"7 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148922947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Indole-3-acetic acid as a resistance elicitor in tomato: effects on secondary metabolites and demographic characteristics of the tomato leaf miner 吲哚-3-乙酸对番茄叶螨次生代谢物和种群特征的影响

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2026-09-01 DOI: 10.1007/s10340-026-02099-x

Elahe Rostami,Azim Nemati,Vahid Mahdavi,Zahra Afrazeh,Yu Zhang,Lei Li,Qing Zhao,Ke Jian Lin,Shujing Gao

引用次数: 0

Nitrogen fertilization influences morphology and macronutrient content in the corn leafhopper Dalbulus maidis 施氮对玉米叶蝉形态和常量养分含量的影响

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2026-09-01 DOI: 10.1007/s10340-026-02101-6

Nicolás A. Melchert,María del Pilar Medina Pereyra,Eduardo G. Virla,Erica Luft-Albarracin

引用次数: 0

Transitioning stored-grain pest control: moving from traditional fumigants to plant-derived alternatives 过渡储粮虫害防治：从传统熏蒸剂转向植物衍生替代品

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2026-09-01 DOI: 10.1007/s10340-026-02093-3

Adrien Guverinoma,Shiyuan Miao,Adria Nirere,Precious Asumadu,Gerard Hakizimana

引用次数: 0

Squash trichome traits are associated with Bemisia tabaci abundance and treatment efficacy across cultivars 南瓜毛状性状与不同品种烟粉虱的丰度和防治效果有关

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2026-09-01 DOI: 10.1007/s10340-026-02092-4

George N. Mbata,Sanower Warsi,Jermaine Perier,Michael D. Toews,David Shapiro-Ilan,Alvin M. Simmons

引用次数: 0

Global climatic potential distribution of Spodoptera litura under current and future climates 斜纹夜蛾在当前和未来气候下的全球气候势分布

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2026-09-01 DOI: 10.1007/s10340-026-02100-7

Satoshi Kawakita,Darren J. Kriticos,Myron P. Zalucki

引用次数: 0

The impact of high temperatures on Scaphoideus titanus demographic parameters 高温对泰坦星种群参数的影响

IF 4.8 1区农林科学

Journal of Pest Science Pub Date : 2026-09-01 DOI: 10.1007/s10340-026-02090-6

Stefan Cristian Prazaru,Davide Scaccini,Laura Rosso,Filippo Pedrollo,Charlotte Gonzalez Dehennault,Lisa D’Ambrogio,Alberto Pozzebon,Carlo Duso

{"title":"The impact of high temperatures on Scaphoideus titanus demographic parameters","authors":"Stefan Cristian Prazaru,Davide Scaccini,Laura Rosso,Filippo Pedrollo,Charlotte Gonzalez Dehennault,Lisa D’Ambrogio,Alberto Pozzebon,Carlo Duso","doi":"10.1007/s10340-026-02090-6","DOIUrl":"https://doi.org/10.1007/s10340-026-02090-6","url":null,"abstract":"Abstract Temperature can affect the survival, the development, and the phenology of Scaphoideus titanus Ball (Hemiptera: Cicadellidae), the vector of Flavescence dorée phytoplasma (FDp). To assess the potential effects of global warming on this vector, three complementary experiments were conducted under controlled conditions. In the first, nymphs were reared under four fluctuating thermal regimes simulating past, present, and future climatic scenarios. Developmental times decreased with higher temperatures, but also survival, adult longevity, and total lifespan declined. Under the “warmest” regime treatment (+ 6 °C above baseline), total nymphal duration decreased by up to 40%, but mortality rose significantly. In the second experiment, adults were exposed for 4 h at 32, 36, and 40 °C. Their mortality increased with temperature, particularly among males. The third experiment, using 1-h exposures to constant temperatures (35–50 °C), confirmed sex-specific differences: females exhibited higher LT 50 (37.5–41 °C) and LT 99 (45–50 °C) values than males, indicating greater thermal tolerance. These results demonstrate that although warming accelerates development, it simultaneously increases mortality, potentially limiting population growth in the warmest vineyard areas while enabling expansion to cooler ones. The inclusion of fluctuating thermal regimes represents a novel approach, providing realistic insights into S. titanus responses to climate change. Findings highlight the importance of incorporating temperature dynamics into predictive and integrated pest management (IPM) models and suggest that vineyard microclimate manipulation through canopy management or trunk barriers could mitigate heat stress and affect vector abundance under future warming scenarios.","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"17 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148922950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0