Nitenpyram resistance and IR56 feeding jointly drive the wing dimorphism in Nilaparvata lugens

IF 4.3 1区 农林科学 Q1 ENTOMOLOGY
Ruifang Chen, Huihui Zhang, Jianzheng Zou, Jiayi Chen, Zewen Liu
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Abstract

Nilaparvata lugens Stål is a significant insect pest of rice throughout Asia, and the use of insect-resistant rice varieties has been proposed as a key strategy to control this pest, and reduce reliance on insecticides. However, little is known about the interplay between these two methods, particularly their combined influence on the behavior, development, and reproduction. In this study, we found that both nitenpyram resistance in BPH and resistance in the rice variety to BPH jointly regulated wing dimorphism. A large proportion of long-winged BPHs (78%) were observed in the nitenpyram-resistant strain (NitR) when feeding on IR56, a BPH-resistant rice variety. No similar result in SUS (susceptible) insects was observed on either IR56 or TN1, nor in NitR insects on TN1. These results suggest that nitenpyram resistance and feeding on IR56 together regulated BPH wing dimorphism. Through quantitative analysis of hormone and insulin pathway genes, we found that a decrease in juvenile hormone (JH) and/or an increase in ecdysone (20E) induced the long-winged morph, accompanied by a reduction in the expression of insulin receptor 2 (InR2). Alterations in hormone levels and silencing InR2 could regulate wing morph differentiation. In males, JH III addition decreased long-wing proportion from 72 to 42%, while silencing InR2 increased long-wing proportion from 27 to 71%. This study suggests a potential risk that the insect-resistant rice variety may promote the spread of resistant insects, creating a contradiction in the combined use of insecticides and insect-resistant varieties.

耐虫性和IR56取食共同驱动褐飞虱翅膀二型性
褐飞虱(Nilaparvata lugens stamatl)是亚洲地区重要的水稻害虫,利用抗虫水稻品种是防治褐飞虱和减少对杀虫剂依赖的关键策略。然而,人们对这两种方法之间的相互作用知之甚少,特别是它们对行为、发育和繁殖的综合影响。在本研究中,我们发现褐飞虱对烯虫啉的抗性和水稻品种对褐飞虱的抗性共同调控了翅膀的二型现象。结果表明,抗尼氏锥体株系(NitR)取食抗尼氏锥体水稻品种IR56时,长翅飞虱的比例较高(78%)。在IR56和TN1上,SUS(敏感)昆虫没有观察到类似的结果,在TN1上,NitR昆虫也没有观察到类似的结果。这些结果表明,耐硝吡虫啉和摄食IR56共同调节了BPH翅膀的二态性。通过对激素和胰岛素途径基因的定量分析,我们发现少年激素(JH)的减少和/或脱皮激素(20E)的增加诱导了长翼形态,同时伴随着胰岛素受体2 (InR2)的表达减少。激素水平的改变和InR2的沉默可以调节翅膀形态的分化。添加jhiii使雄虫长翼比例从72%降低到42%,而沉默InR2使雄虫长翼比例从27%提高到71%。这项研究提示了一个潜在的风险,即抗虫水稻品种可能会促进抗虫昆虫的传播,从而在杀虫剂和抗虫品种的联合使用中产生矛盾。
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来源期刊
Journal of Pest Science
Journal of Pest Science 生物-昆虫学
CiteScore
10.40
自引率
8.30%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.
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