Ruifang Chen, Huihui Zhang, Jianzheng Zou, Jiayi Chen, Zewen Liu
{"title":"Nitenpyram resistance and IR56 feeding jointly drive the wing dimorphism in Nilaparvata lugens","authors":"Ruifang Chen, Huihui Zhang, Jianzheng Zou, Jiayi Chen, Zewen Liu","doi":"10.1007/s10340-025-01911-4","DOIUrl":null,"url":null,"abstract":"<p><i>Nilaparvata lugens</i> Stål is a significant insect pest of rice throughout Asia, and the use of insect-resistant rice varieties has been proposed as a key strategy to control this pest, and reduce reliance on insecticides. However, little is known about the interplay between these two methods, particularly their combined influence on the behavior, development, and reproduction. In this study, we found that both nitenpyram resistance in BPH and resistance in the rice variety to BPH jointly regulated wing dimorphism. A large proportion of long-winged BPHs (78%) were observed in the nitenpyram-resistant strain (NitR) when feeding on IR56, a BPH-resistant rice variety. No similar result in SUS (susceptible) insects was observed on either IR56 or TN1, nor in NitR insects on TN1. These results suggest that nitenpyram resistance and feeding on IR56 together regulated BPH wing dimorphism. Through quantitative analysis of hormone and insulin pathway genes, we found that a decrease in juvenile hormone (JH) and/or an increase in ecdysone (20E) induced the long-winged morph, accompanied by a reduction in the expression of insulin receptor 2 (<i>InR2</i>). Alterations in hormone levels and silencing <i>InR2</i> could regulate wing morph differentiation. In males, JH III addition decreased long-wing proportion from 72 to 42%, while silencing <i>InR2</i> increased long-wing proportion from 27 to 71%. This study suggests a potential risk that the insect-resistant rice variety may promote the spread of resistant insects, creating a contradiction in the combined use of insecticides and insect-resistant varieties.</p>","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"50 1","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pest Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10340-025-01911-4","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Nilaparvata lugens Stål is a significant insect pest of rice throughout Asia, and the use of insect-resistant rice varieties has been proposed as a key strategy to control this pest, and reduce reliance on insecticides. However, little is known about the interplay between these two methods, particularly their combined influence on the behavior, development, and reproduction. In this study, we found that both nitenpyram resistance in BPH and resistance in the rice variety to BPH jointly regulated wing dimorphism. A large proportion of long-winged BPHs (78%) were observed in the nitenpyram-resistant strain (NitR) when feeding on IR56, a BPH-resistant rice variety. No similar result in SUS (susceptible) insects was observed on either IR56 or TN1, nor in NitR insects on TN1. These results suggest that nitenpyram resistance and feeding on IR56 together regulated BPH wing dimorphism. Through quantitative analysis of hormone and insulin pathway genes, we found that a decrease in juvenile hormone (JH) and/or an increase in ecdysone (20E) induced the long-winged morph, accompanied by a reduction in the expression of insulin receptor 2 (InR2). Alterations in hormone levels and silencing InR2 could regulate wing morph differentiation. In males, JH III addition decreased long-wing proportion from 72 to 42%, while silencing InR2 increased long-wing proportion from 27 to 71%. This study suggests a potential risk that the insect-resistant rice variety may promote the spread of resistant insects, creating a contradiction in the combined use of insecticides and insect-resistant varieties.
期刊介绍:
Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues.
Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates.
Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management.
Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.