Journal of Evolutionary Biochemistry and Physiology最新文献

{"title":"Expression Profile of Isogenic Early Mesodermal Cells Differentiated from Human Induced Pluripotent Stem Cells","authors":"A. V. Selezneva, E. V. Korobko, S. L. Kiselev, Yu. G. Suzdaltseva","doi":"10.1134/s0022093024020042","DOIUrl":"https://doi.org/10.1134/s0022093024020042","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Scar formation during normal regeneration of damaged tissue\u0000can lead to noticeable cosmetic and functional defects of organs\u0000and thus significantly affect the quality of life. Meanwhile, fetal\u0000tissues before the third trimester of pregnancy are known to be\u0000capable of complete regeneration with the restoration of the original\u0000architecture and functional activity. Understanding the cellular\u0000and molecular mechanisms of fetal wound regeneration will provide\u0000the basis for the development of successful treatments aimed to minimize\u0000scarring. Mesenchymal stromal cells (MSCs) play an important role\u0000in tissue repair, since cytokines, chemokines, growth factors and\u0000extracellular vesicles they secrete are involved in the regulation\u0000of migration, angiogenesis, synthesis, and remodeling of the extracellular\u0000matrix. Mesodermal differentiation of human induced pluripotent\u0000stem cells (iPSCs) enables to reproduce consecutive stages of embryogenesis\u0000in vitro and to create isogenic cell models of MSCs, corresponding\u0000to different stages of human development. Here, we performed a specifically\u0000directed, multistage, mesodermal differentiation of iPSCs into isogenic\u0000cell lines of the primitive streak, lateral and paraxial mesoderm,\u0000as well as carried out a comparative analysis of their expression\u0000profiles. It was shown that the derived cells of the lateral mesoderm\u0000(LM) and paraxial mesoderm (PM) are precursors for MSCs. The MSCs,\u0000derived due to differentiation of both LM and PM cells, shared a\u0000similar expression profile of pan-mesodermal markers. A comparative\u0000analysis of the functional activity of MSCs and their precursors\u0000in a pro-inflammatory microenvironment will hopefully provide molecular\u0000tools for a better insight into the basic mechanisms of fetal tissue\u0000regeneration and help identify therapeutic targets to minimize scarring\u0000and pathological processes characterized by excessive fibroplasia.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"23 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-Analysis of Experimental Studies of Diet-Dependent Effects of Melatonin Monotherapy on Body Weight Gain and Eating Behavior in Rats","authors":"N. V. Kuzmenko, V. A. Tsyrlin, M. G. Pliss, M. M. Galagudza","doi":"10.1134/s0022093024020121","DOIUrl":"https://doi.org/10.1134/s0022093024020121","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In a previous meta-analysis, we showed that long-term melatonin\u0000administration minimizes diet-induced changes in lipid profiles.\u0000The present work was aimed to use a meta-analysis to study the diet-denendent\u0000effect of melatonin monotherapy on body weight gain (BWG) and eating\u0000behavior in rats. We carried out a meta-analysis of 43 publications\u0000examining the effects of melatonin monotherapy on BWG, blood leptin\u0000levels, food and water intake in rats fed a standard diet (37 publications),\u0000as well as high-fructose (5 publications) and high-fat (9 publications)\u0000diets. Our findings suggest that although melatonin reduced triglyceride,\u0000total cholesterol and leptin levels in rats fed high-fructose and\u0000high-fat, but not a standard, diets, melatonin therapy prevented\u0000BWG in rats on either diet. Increasing the dose of melatonin did\u0000not enhance the effect of therapy on any diet. We did not obtain\u0000convincing evidence that the effect of melatonin depends on the\u0000initial body weight and age of rats. On a standard diet, melatonin has\u0000been found to reduce food and water intake. Melatonin did not reduce\u0000BWG with a standard diet when administered intraperitoneally. Our\u0000research suggests that melatonin may be beneficial in diet-induced\u0000metabolic disorders as it improves lipid profiles and prevents BWG.\u0000However, a decrease in appetite and BWG when taking melatonin may\u0000also be due to negative side effects of the drug, which requires\u0000further research.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"10 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Cold on Adrenoreactivity of the Rat Superior Mesenteric Artery","authors":"V. N. Yartsev","doi":"10.1134/s0022093024020200","DOIUrl":"https://doi.org/10.1134/s0022093024020200","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The effect of cold on the reactivity of the rat superior mesenteric\u0000artery (SMA), estimated by the magnitude of contractile responses\u0000of its segments to noradrenaline (NA, 0.01–10.0 µM) and electrical field\u0000stimulation (EFS) of the perivascular nerves at a frequency of 3,\u000010 and 40 Hz in both the absence and presence of NA in the medium\u0000at a temperature of 37 or 25°C, was studied in isometric experiments. Cooling\u0000did not alter SMA tone evoked by NA at all its concentrations used.\u0000In both the absence and presence of low NA concentrations (0.01–0.10\u0000µM), cooling led to a significant decrease in the neurogenic SMA\u0000response at all EFS frequencies, while in the presence of NA at\u0000a high concentration (1–10 µM), cooling had no significant effect\u0000on this response. In the presence of NA at a low concentration,\u0000cooling insignificantly (at low EFS frequencies) and significantly\u0000(at high EFS frequencies) reduced the potentiating effect of NA\u0000on the neurogenic SMA constriction. At a high concentration under\u0000normal temperature conditions (37°C), NA reduced the neurogenic\u0000SMA constriction at all EFS frequencies, while under cooling conditions\u0000(25°C), it had a potentiating effect at low and a depressing effect\u0000at high EFS frequencies. These results demonstrate that the depressing\u0000effect of cold on the neurogenic constriction of the rat SMA, occurring\u0000at low and disappearing at high NA concentrations, as well as on\u0000the NA-evoked potentiation of the neurogenic vasoconstriction, is irrelevant\u0000to a decrease in the contractile effect of NA, which is the main\u0000neurotransmitter in this artery. These effects may contribute to\u0000blood rerouting from the surface to the deeper regions of the body,\u0000thus reducing heat loss and improving thermoregulation.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"101 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Transcription Factor NF-κB in Neuroimmunoendocrine Mechanisms of Respiratory Diseases","authors":"Yu. I. Belova, E. S. Mironova, T. S. Zubareva, I. M. Kvetnoy, P. K. Yablonsky","doi":"10.1134/s0022093024020285","DOIUrl":"https://doi.org/10.1134/s0022093024020285","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>One of the current challenges of biomedicine is to elucidate\u0000the multicomponent and multilevel mechanism of integrated neuroimmunoendocrine\u0000regulation of physiological functions. Respiratory diseases are\u0000leading in the structure of general morbidity among the population\u0000and remain one of the most relevant problems of modern healthcare.\u0000Numerous risk factors can contribute to the development of such\u0000pulmonary pathologies as pneumonia, lung cancer, asthma, chronic\u0000obstructive pulmonary disease, acute respiratory distress syndrome,\u0000pulmonary fibrosis and others, whose incidence tends to increase\u0000annually. In this regard, signaling molecules, which are involved\u0000in neuroimmunoendocrine respiratory regulation and can be considered\u0000as both prognostic biomarkers and potential therapeutic targets,\u0000are coming into focus of modern translational biomedicine. This\u0000review addresses the role of one of the key actors in the neuroimmunoendocrine\u0000regulation of homeostasis, a ubiquitous transcription factor NF-κB,\u0000in the regulation of respiratory function in health and during the\u0000pathogenesis of lung diseases. There is ample evidence that many\u0000pulmonary pathologies, resulting from ongoing inflammatory processes,\u0000are associated with NF-κB activation. A comprehensive investigation\u0000of the mechanisms underlying NF-κB activation and its relationship\u0000with other signaling pathways will contribute to solving the main\u0000task of translational biomedicine, the development of innovative approaches\u0000to prevention and personalized targeted therapies for human socially\u0000significant pathologies, including respiratory diseases.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"20 2 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140886540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-Related Dynamics of Insulin Signaling in Neurons of the Rat Hypothalamic Tuberal Nuclei","authors":"P. A. Anfimova, V. V. Porseva, L. G. Pankrasheva, E. S. Shirina, P. M. Masliukov","doi":"10.1134/s0022093024020303","DOIUrl":"https://doi.org/10.1134/s0022093024020303","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The hypothalamus is a regulatory center that controls homeostasis,\u0000reproduction, circadian rhythms, and the endocrine system. It is\u0000also involved in the regulation of aging. The expression of insulin receptors\u0000(INR) and insulin receptor substrate 1 (IRS1) was studied in neurons\u0000of the arcuate (ARN), dorsomedial (DMN), and ventromedial (VMN)\u0000tuberal nuclei of the rat hypothalamus in young (2 months), adult\u0000(12 months), and old (24 months) male Wistar rats using immunofluorescence\u0000staining and Western blotting. The results showed that INR and IRS1\u0000expression in neurons of the mediobasal hypothalamic nuclei changes\u0000in opposite directions with age. INR expression decreases in the\u0000ARN and increases in the DMN and VMN, while IRS1 expression is unchanged\u0000in the ARN and VMN and decreases in the DMN.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"148 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140886369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotoxic Stress As a Trigger of Endothelial Dysfunction in Wistar Rats: a Molecular Genetic Study","authors":"M. Yu. Sinitsky, A. V. Sinitskaya, M. V. Khutornaya, M. A. Asanov, D. K. Shishkova, A. O. Poddubnyak, A. V. Ponasenko","doi":"10.1134/s002209302402025x","DOIUrl":"https://doi.org/10.1134/s002209302402025x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Atherosclerosis and coronary artery disease are the leading\u0000causes of disability and mortality among the population. Genotoxic\u0000stress can be potentially considered as a new trigger of endothelial\u0000dysfunction underlying the pathogenesis of these diseases. This\u0000research was aimed to the study of molecular and genetic markers\u0000of genotoxic stress-induced endothelial dysfunction in normolipidemic\u0000Wistar rats. The study was carried out on male Wistar rats injected\u0000intravenously (tail vein) with the alkylating mutagen mitomycin\u0000C (MMC) at a dose of 0.5 mg/kg body weight (experimental group)\u0000or 0.9% NaCl solution (control group) three times a week for a month.\u0000Genotoxic stress in animals was assessed using a micronucleus assay\u0000in polychromatophilic erythrocytes (PCE); endothelial dysfunction\u0000was identified by assessing the expression of <i>Vcam1</i>, <i>Icam1</i>, <i>Sele</i>, <i>Selp</i>, <i>Il6</i>, <i>Ccl2</i>, <i>Cxcl1</i>, <i>Mif</i>, <i>Vwf</i>, <i>Serpine1</i>, <i>Plau</i>, <i>Plat</i>, <i>Klf2</i>, <i>Klf4</i>, <i>Nfe2l2</i>, <i>Nos3</i>, <i>Snai1</i>, <i>Snai2</i>, <i>Twist1</i>, <i>Zeb1</i>, <i>Cdh5</i>,\u0000and <i>Cdh2</i> genes in the endothelial monolayer\u0000of the descending aorta. Rats of the experimental group developed\u0000pronounced genotoxic stress, as evidenced by a more than threefold\u0000increase in the frequency of micronucleated PCE and a decreased\u0000proportion of PCE in the total pool of erythrocytes analyzed. Gene\u0000expression profiling showed that rats of the experimental group\u0000exhibited a pro-inflammatory activation of the endothelium, accompanied\u0000by increased expression of <i>Vcam1</i>, <i>Icam1</i>, <i>Selp</i>, <i>Il6</i>, <i>Ccl2</i> and <i>Cxcl1</i> genes, as well as impaired\u0000endothelial mechanotransduction characterized by decreased expression\u0000of <i>Klf2</i> and <i>Klf4</i> genes. Thus, MMC-induced genotoxic\u0000stress in normolipidemic Wistar rats is associated with the two key\u0000pathogenic links of endothelial dysfunction and can be considered\u0000as one of its triggers.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"14 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140886694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Changes in Aqp4 Gene Expression in the Rat Hypothalamus under Different Drinking Regimens and Arterial Hypertension","authors":"A. A. Evtushenko, I. V. Orlov, I. P. Voronova, T. V. Kozyreva","doi":"10.1134/s0022093024020224","DOIUrl":"https://doi.org/10.1134/s0022093024020224","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Aquaporin-4 (AQP4) is the main water channel in the central\u0000nervous system. AQP4 is highly expressed in brain structures, suggesting\u0000its crucial role in water transport in norm and in pathology. Here,\u0000we studied the effect of a change in the drinking regimen (water\u0000deprivation, hyperhydration) and inherited arterial hypertension\u0000on the expression of the AQP4 water channel gene in the hypothalamus,\u0000a regulatory brain center of visceral functions. The expression\u0000level of the <i>Aqp4</i> gene in hyperhydrated animals\u0000was found to be more than 1.5 times lower than in water-deprived\u0000animals, which may reflect the need to prevent cytotoxic edema during\u0000increased fluid intake. Rats with inherited stress-induced arterial\u0000hypertension are characterized by an increased level of the <i>Aqp4</i> gene in the hypothalamus, suggesting\u0000the involvement of this channel in processes associated with the\u0000regulation of brain water balance and the prevention of vasogenic\u0000cerebral edema in arterial hypertension. Thus, the presence of the\u0000AQP4 water channel in the brain, associated with the protection\u0000of brain cells, is functionally determined by the state of the body,\u0000as evidenced by bidirectional changes in <i>Aqp4</i> expression\u0000during hyperhydration and arterial hypertension.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140886371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Estradiol on Carbohydrate-Fat Metabolism and FGF21 System Activity in Female C57BL/6 Mice with Short-Term Consumption of the Cafeteria Diet","authors":"T. V. Jakovleva, A. Yu. Kazantseva, K. Yu. Mamontova, N. M. Bazhan","doi":"10.1134/s0022093024020315","DOIUrl":"https://doi.org/10.1134/s0022093024020315","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The cafeteria diet contributes to the development of obesity\u0000and metabolic syndrome, reduces insulin sensitivity and glucose\u0000tolerance. Hepatic hormone fibroblast growth factor 21 (FGF21) promotes adaptation\u0000to the consumption of sweet and fatty foods. Female mice are less\u0000sensitive to the damaging effects of the cafeteria diet than males,\u0000which may be due to the effect of estradiol on the activity of the FGF21\u0000system: on the hepatic expression of the <i>Fgf21</i> gene,\u0000on the blood level of hormone, or on the levels of receptors and\u0000coreceptors beta-klotho, which determine the sensitivity of tissues\u0000to FGF21. The purpose of this work was to verify this assumption.\u0000The effect of estradiol (10 µg/animal once every three days) was\u0000evaluated in ovariectomized female C57BL/6 mice who consumed a cafeteria\u0000diet (standard food, lard and cookies) for two weeks. Indicators\u0000of carbohydrate-fat metabolism, taste preferences, and activity\u0000of the FGF21 system were determined. Ovariectomy increased body\u0000weight and subcutaneous adipose tissue weight, fat intake, <i>Pomc</i> expression in the hypothalamus,\u0000decreased expression of estradiol receptors in the liver and cookie\u0000consumption. Estradiol did not have a significant effect on these parameters.\u0000In ovariectomized females with estradiol deficiency, blood cholesterol\u0000levels and liver expression of the glucose-6-phosphatase gene were\u0000lower than in sham operated females, and estradiol normalized these\u0000parameters. Ovariectomy lowered, and the administration of estradiol\u0000increased the level of coreceptor beta-klotho (<i>Klb</i>)\u0000mRNA in the liver and in the hypothalamus. These results suggest that\u0000at the initial stages of consumption of sweet and fatty foods, estradiol\u0000increases the sensitivity of the liver and hypothalamus to FGF21\u0000and thereby enhances the contribution of the FGF21 system to the processes\u0000of adaptation to the cafeteria diet.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"62 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140886277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Functional State of Glutamate- and GABA-Ergic Neurons in the Inferior Colliculi of Krushinsky–Molodkina Rats at Early Stages of Epileptogenesis","authors":"S. D. Nikolaeva, L. S. Nikitina, M. V. Glazova, V. T. Bakhteeva, E. V. Chernigovskaya","doi":"10.1134/s0022093024020212","DOIUrl":"https://doi.org/10.1134/s0022093024020212","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Disturbances in the neurotransmitter systems associated with\u0000the development of temporal lobe epilepsy (TLE) have been well-studied\u0000in forebrain structures including the temporal cortex, amygdala, and\u0000hippocampus. It is known that in rodents genetically prone to audiogenic\u0000seizures, TLE establishment induced by audiogenic kindling is also\u0000associated with the involvement of the forebrain, however, initially\u0000epileptiform activity arises in the brainstem, in particular, in\u0000the inferior colliculi (IC). It is reasonable to suppose that neurochemical\u0000alterations in the IC may contribute significantly to the spreading\u0000of epileptiform activity at early steps of TLE development. At the\u0000same time, the functional state of the key neurotransmitter systems\u0000in the IC of audiogenic rodents exposed to audiogenic kindling remains\u0000unknown. In the present work, we performed a comprehensive analysis\u0000of proteins involved in glutamate- and GABA-ergic transmission in\u0000the IC of Krushinsky–Molodkina (KM) rats genetically prone to audiogenic\u0000seizures. A modified audiogenic kindling protocol was used to reproduce\u0000the early stage of TLE development. According to this protocol,\u0000rats were exposed to daily audiogenic stimulations for seven days.\u0000Naive KM rats were used as a control. Although the rodent’s predisposition to\u0000audiogenic seizures is often associated with the impairment of GABAergic\u0000transmission, no significant changes were found in the expression\u0000of GABA synthesis enzymes and GABA_A receptor\u0000α1 subunit in the IC of KM rats either 24 hours or a week after\u0000their last seizure. However, 24 hours after the last audiogenic\u0000stimulation, an increase in glutamatergic transmission in the IC\u0000was observed including upregulation of ERK 1/2 kinases, synaptic\u0000proteins synapsin 1 and SV2B, VGLUT1 and VGLUT2. One week after\u0000the last seizure, only an increase in VGLUT1 content in the IC was\u0000observed, suggesting the persistent changes in the neurons of forebrain\u0000structures, in particular, of the temporal cortex.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"52 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Hypernatremia on Protein Reabsorption in Renal Proximal Tubules of the Lake Frog Pelophylax ridibundus","authors":"N. P. Prutskova, E. V. Seliverstova","doi":"10.1134/s0022093024020091","DOIUrl":"https://doi.org/10.1134/s0022093024020091","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Although protein reabsorption in the renal proximal tubules\u0000is concurrent with ion and water transport, little is known about\u0000the dependence of receptor-mediated protein endocytosis on changes\u0000in water and salt balance. The aim of the study was to investigate\u0000tubular reabsorption and intracellular vesicular transport of various\u0000proteins in a lake frog (<i>Pelophylax ridibundus</i>)\u0000model of hypernatremia. The frogs were injected with hypertonic\u0000sodium chloride solution (0.75 M NaCl) 1 h before the administration\u0000of green or yellow fluorescent proteins (GFP or YFP), as well as\u0000lysozyme. Fluorescent immunohistochemistry and confocal microscopy\u0000were used to detect lysozyme and the endocytic receptor megalin\u0000in the epithelial cells of proximal tubules. The endocytosis dynamics\u0000was assessed by automated quantification of colocalized fluorescent\u0000signals of megalin and injected proteins. We found a statistically\u0000significant decrease in the reabsorption of GFP, YFP, and lysozyme\u0000in proximal tubules after NaCl injection. The accumulation of proteins\u0000in early endosomes and the decrease in their entry into late endosomes\u0000and lysosomes suggest a delay in the intracellular vesicular transport\u0000of proteins under hypernatremia. The data obtained are analyzed\u0000as related to changes in blood parameters and kidney activity during\u0000osmoregulation, as well as to the role of chloride channels in receptor-mediated\u0000protein endocytosis. We assume that increased ion transport in proximal\u0000tubular epitheliocytes under hypernatremia conditions leads to a\u0000decreased protein reabsorption capacity of these cells and delayed intracellular\u0000transport of proteins.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"57 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}