Journal of Evolutionary Biochemistry and Physiology最新文献

{"title":"A Comparative Study of Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Models in Immature and Prepubertal Female Rats","authors":"A. S. Pechalnova, К. В. Деркач, I. Yu. Morina, И. И. Зорина, L. V. Bayunova, И. В. Романова, E. E. Chernenko, А. О. Шпаков","doi":"10.1134/s0022093025060031","DOIUrl":"https://doi.org/10.1134/s0022093025060031","url":null,"abstract":"Polycystic ovary syndrome (PCOS), one of the most common endocrine disorders in women, leads to a reduction in reproductive potential and infertility. To study PCOS, rodent models are used, including those induced by dehydroepiandrosterone (DHEA). However, they are not sufficiently studied, including in terms of ovulation stimulation in PCOS rats with luteinizing hormone (LH) agonists, and this complicates their use for developing approaches to treat this pathology. This work was aimed to comparatively study and characterize morphological and biochemical parameters in animals using the two most popular PCOS models, induced by a six-week treatment of immature (aged 23–25 days) and a three-week treatment of prepubertal (aged 45–47 days) female rats with DHEA, as well as to assess the effectiveness of ovulation stimulation in PCOS rats using TP03, a low-molecular-weight LH receptor agonist developed in our laboratory. A six-week DHEA treatment of immature female rats led to the development of polycystic ovary morphology, a decrease in blood progesterone levels and the expression of ovarian genes encoding key steroidogenic proteins (CYP11A1, CYP17A1) and the metalloproteinase ADAMTS-1, a marker of ovulation. TP03 treatment increased progesterone levels, enhanced the expression of the Cyp11a1, Cyp17a1, and Adamts-1 genes, and led to the formation of corpora lutea (CL), with a significant proportion of them being represented by luteinized unruptured follicles. A three-week DHEA treatment of prepubertal females also led to characteristic features of PCOS, but had a lesser effect on Adamts-1 expression and did not cause the formation of defective CL upon TP03 stimulation. In this case, two subgroups were identified among PCOS rats c with relatively low and high levels of progesterone. They differed significantly in Cyp17a1 expression and the effectiveness of new cycle’s CL formation after TP03 treatment. Thus, the longer-term PCOS model induced by DHEA in immature female rats is well suited for studying functional disorders in severe forms of PCOS, while the shorter-term PCOS model generated in prepubertal rats by a three-week DHEA treatment is more suitable for studying moderately severe forms of PCOS and optimizing the choice of ovulation stimulants effective for this disease.","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"61 6","pages":"1692-1705"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulation of Ovarian Steroidogenesis and Ovulation in Mature Female Rats with Type 2 Diabetes Mellitus","authors":"К. В. Деркач, I. Yu. Morina, A. S. Pechalnova, L. V. Bayunova, И. И. Зорина, Marina Ryzhova, И. В. Романова, А. О. Шпаков","doi":"10.1134/s0022093025060110","DOIUrl":"https://doi.org/10.1134/s0022093025060110","url":null,"abstract":"Type 2 diabetes mellitus (T2DM) negatively impacts the female reproductive system, however, hormonal and functional disturbances in the ovaries and their response to luteinizing hormone receptor (LHR) agonists in T2DM have been poorly studied, and data on allosteric LHR-agonists are lacking. The aim of the study was to evaluate the effect of the allosteric LHR-agonist TP03 and human chorionic gonadotropin (hCG) on ovarian steroidogenesis and ovulation in female rats with diet-induced T2DM. To induce ovulation, rats were administered Follimag (15 IU/rat) and Cetrotide (100 µg/rat, twice) in diestrus and TP03 (25 mg/kg) or hCG (25 IU/rat) 48 h later, analyzing the parameters 8 or 24 h after the injection of LHR-agonists. In T2DM rats, estradiol levels in the blood, the number of preovulatory follicles, and the expression of the ADAMTS-1 metalloproteinase gene, an ovulation marker, were decreased. Treatment with TP03 and hCG increased progesterone levels (after 8 hours) and the expression of the ovarian genes Cyp17a1 (hCG, TP03) and Star (hCG), responsible for ovarian steroidogenesis, as well as the Adamts-1 gene. After 24 hours, corpora lutea were detected in the ovaries of T2DM rats, with hCG and TP03 being comparable in this parameter. Compared to controls, T2DM rats maintained reduced estradiol levels, and the stimulating effect of LHR-agonists on ovarian gene expression was less pronounced. Thus, in T2DM, TP03 is not inferior to hCG in its ability to stimulate ovarian steroidogenesis and ovulation, and this indicates the prospects for its use in assisted reproductive technologies for T2DM.","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"61 6","pages":"1818-1830"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147334139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucagon-Like Peptide-1 Receptor Agonists Are Promising for the Treatment of Brain Diseases: an Outlook from the Perspective of Integrative Physiology","authors":"N. V. Gulyaeva","doi":"10.1134/s0022093025050023","DOIUrl":"https://doi.org/10.1134/s0022093025050023","url":null,"abstract":"Abstract Diabetes is one of the main risk factors for the development of cognitive dysfunction. Insulin resistance and chronic hyperglycemia cause a cascade of pathological events in the brain, including neuroinflammation, impaired neurotransmitter systems and energy metabolism, oxidative stress, and vascular pathologies that ultimately lead to neurodegeneration, loss of synaptic plasticity, brain aging, cognitive impairment, and dementia. The glucagon-like peptide (GLP–1) regulates food consumption and appetite, and its analogs, GLP-1 receptor agonists (GLP-1RAs), are used to treat type 2 diabetes, obesity, and metabolic syndrome. However, an increasing number of research studies are demonstrating that these substances also have neuroprotective effects in neurodegenerative diseases, mental disorders, and other brain conditions. Analysis of the effects of GLP-1 and GLP-1RAs on cognitive, psychometric, cellular, and molecular changes in preclinical and clinical studies shows that these compounds and drugs modulate the molecular and cellular changes that determine the phenomenology of many brain diseases. By activating GLP-1 receptors, natural GLP-1 and GLP-1RAs reduce insulin resistance, suppress neuroinflammation and oxidative stress, and prevent negative changes in neuroplasticity. The review considers the repurposing of GLP-1Ras from the perspective of integrative physiology, summarizes modern concepts about the mechanisms of action of GLP-1RAs according to the results of experimental studies and presents current clinical data on the use of GLP-1RAs in patients with brain diseases. The use of GLP-1RA is a new therapeutic strategy that addresses both metabolic and cognitive/neuropsychiatric aspects, which is fundamentally important for the treatment of comorbid diseases.","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"61 5","pages":"1326-1348"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0022093025050023.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Dendrobium Officinale Extract on the PI3K/Akt Pathway in Mice with Ulcerative Colitis and Its Mechanism of Action","authors":"Mingwen Jia, Zifu Hong, Yufei Li, E Li, Ang Li, Yiping Li, Zhen Liu, Muwen Qu","doi":"10.1134/s1234567825600294","DOIUrl":"https://doi.org/10.1134/s1234567825600294","url":null,"abstract":"This study was designed to evaluate the effect of Dendrobium officinale (DO) extract on the PI3K/Akt signaling cascade in a murine model of ulcerative colitis (UC) and to explore the underlying mechanisms. For this sixty male BALB/c mice with comparable weights were randomly divided into six groups: a normal control, a DSS-induced UC model, a positive control treated with 5-aminosalicylic acid (5-ASA), and three groups receiving DO extract at low (200 mg·kg–1), medium (400 mg·kg–1), and high (800 mg·kg–1) doses, with 10 mice per group. The effects of DO extract on body weight and colon length were observed, as well as its impact on disease activity index (DAI) score, serum cytokine (IL-1β, IL-6, TNF-α) levels, histopathological changes in the colon, and the expression of phosphorylated PI3K and Akt proteins through western blot analysis. It was found that DSS-induced UC mice exhibited higher DAI scores, IL-1β, IL-6, and TNF-α levels, and histopathological injury scores. After treatment, DO extract at different doses reduced DAI scores and histopathological injury scores, lowered serum IL-1β, IL-6, and TNF-α levels, and decreased the expression of p-PI3K and p-Akt proteins, leading to improvement of colon injury. This study suggests that DO therapeutic effects on DSS-induced UC mice may exert its effects via the PI3K/Akt pathway.","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"61 5","pages":"1506-1515"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility of Different Nicotinamide Riboside Doses Administered Intravenously to Rats","authors":"Ekaterina Podyacheva, N. Yu. Semenova, Daria Mukhametdinova, I. А. Zelinskaya, Лада Мурашова, Anatoliya Onopchenko, Е. В. Щелина, M. O. Martynov, Vyacheslav Dyachuk, Vsevolod Zinserling, Yana Toropova","doi":"10.1134/s0022093025020036","DOIUrl":"https://doi.org/10.1134/s0022093025020036","url":null,"abstract":"Nicotinamide riboside (NR) a NAD+ precursor. There is ample evidence in the literature for beneficial effects of its peroral administration in cardiovascular, neurodegenerative, renal, hepatic, and some other diseases. Previously, we have advanced a hypothesis that intravenous NR administration exerts a cardioprotective effect during doxorubicin anticancer chemotherapy. The present study was aimed to assess the biocompatibility of various NR doses during repeated intravenous drug administration to Wistar rats. The doses tested were the following: 150, 300, 450, and 600 mg/kg matching respective cumulative doses of 900, 1800, 2700, and 3600 mg/kg. Under this administration regimen, NR biocompatibility was demonstrated for the doses of 150, 300, and 450 mg/kg. Even at 450 mg/kg, repeated intravenous NR administration revealed no adverse effects on the cardiac parasympathetic ganglia of the autonomic nervous system. However, the dose of 600 mg/kg led to a number adverse side effects, including reduced cardiovascular efficiency, morphological and functional alterations in the myocardium, liver and kidneys, as well as a higher death rate and decreased exercise tolerance in rats.","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"61 2","pages":"396-410"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spinal HDAC2 Influences the Development of Chronic Pancreatitis-Related Pain through mGluR2","authors":"Xiang‐Tian Zeng, Dingwen Zhong, Wenhui Chen, Qixin Dai, Xianyu Huang, Yong He, Rongzhen Ye, Xiulin Xiao, Yonghui Liao","doi":"10.1134/s0022093024600568","DOIUrl":"https://doi.org/10.1134/s0022093024600568","url":null,"abstract":"This study investigates the roles and interactions of spinal histone deacetylase 2 (HDAC2) and glutamate metabotropic receptor 2 (mGluR2) in chronic pancreatitis (CP)-related pain. A CP rat model was induced using dibutyltin dichloride (DBTC). Pain sensitivity was assessed using mechanical and thermal pain tests. HDAC2 and mGluR2 expression levels in the spinal cord were measured using Western blot and qPCR. The effects of HDAC2 inhibition and mGluR2 activation on inflammatory markers and pain sensitivity were also evaluated. Cell co-culture experiments explored regulatory mechanisms involving cholecystokinin (CCK)-stimulated pancreatic acinar cells (AR42J) and neuron-like cells (F11). In this study, HDAC2 was highly expressed and mGluR2 expression was down-regulated in the spinal cords of rats modelling CP. HDAC2 inhibition increased mGluR2 expression, decreased inflammatory cytokines (IL-1β, IL-6, TNF-α, CCL-2) in pancreatic tissue, and reduced pain sensitivity. Activation of mGluR2 similarly alleviated inflammation and pain. Co-culture experiments showed that CCK-stimulated AR42J cells modulated HDAC2 and mGluR2 expression in F11 cells via inflammatory factors. HDAC2 was found to bind and modify the mGluR2 promoter, regulating its expression. In conclusion, HDAC2 and mGluR2 play critical roles in CP-related pain. Modulating their activity effectively reduces pain, providing new insights into CP pain mechanisms and potential therapeutic targets. Further research should explore the specific interactions between HDAC2 and mGluR2 to develop more effective treatments.","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"61 1","pages":"358-367"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of Monoamine Oxidase Involvement in the Development of Hyperbaric Oxygen Seizures","authors":"S. Yu. Zhilyaev, I. N. Basova, T. F. Platonova, O. S. Alekseeva, N. A. Gavrisheva, I. T. Demchenko","doi":"10.1134/s0022093024040161","DOIUrl":"https://doi.org/10.1134/s0022093024040161","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Hyperbaric oxygen (HBO₂) breathing\u0000induces generalized tonic and clonic seizures through poorly understood\u0000mechanisms. The purpose of the research was to evaluate the mechanisms\u0000of involvement of monoamine oxidase (MAO) in the development of\u0000hyperbaric oxygen seizures. In rats placed in a pressure chamber\u0000under an oxygen pressure of 5 ATA, convulsive reactions were analyzed\u0000after the administration of pyrazidol, an MAO-A inhibitor, and pargyline,\u0000an MAO-B inhibitor. Studies have shown a decrease in the activity\u0000of MAO isoforms in HBO₂ as well as a delay\u0000in the development of seizures in animals with inhibition of MAO-A\u0000and MAO-B. The level of GABA in the brain decreased with HBO₂,\u0000and inhibition of MAO-B with pargyline prevented the decrease in\u0000the inhibitory transmitter. The results indicate that MAO isoforms\u0000play an important role in regulating epileptogenesis in extreme\u0000hyperoxia. Hyperbaric oxygen, inhibiting the catalytic activity\u0000of MAO by transforming its molecular structure, leads to disruption\u0000of the regulation of the exchange of monoamine neurotransmitters\u0000and a decrease in the level of GABA in the brain, which together\u0000leads to an imbalance of excitation/inhibition processes in the\u0000central nervous system, which is the basis for the development of oxygen\u0000epilepsy.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"21 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Preservation on Changes in the Volume of Corneal Endothelial Cells in an Environment with a High Concentration of Potassium","authors":"L. E. Katkova, G. S. Baturina, M. M. Teterin, A. I. Sakhanenko, I. G. Palchikova, I. A. Iskakov, E. I. Solenov","doi":"10.1134/s0022093024040331","DOIUrl":"https://doi.org/10.1134/s0022093024040331","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The experimental study of preservation length on the effect\u0000of high potassium concentration in the medium on the volume of human\u0000corneal endothelial cells was done. The results of the study of individual\u0000samples of fragments of donor material and the values calculated\u0000using joined data after hypothermic preservation for 4 and 10 days\u0000are presented. The increase of the time when cornea samples are\u0000kept in preservation medium (Eusol-C) at 4°C led to a decrease in\u0000the average value (<i>M</i> ± <i>SEM</i>) of cell swelling indicator (<i>Q</i>) in a potassium medium from 1.055\u0000± 0.001; <i>n</i> = 982 to 1.014 ±\u00000.001; <i>n</i> = 338; after 4 and\u000010 days, respectively. Student’s <i>t-</i>test\u0000for independent samples showed a high degree of significance for\u0000the difference between these values (<i>p</i> =\u00002E-76). Identification of the proportion of cells capable to swell\u0000in a medium with a high content of potassium ions (<i>Q</i> ≥ 1), reflecting the electrogenic activity\u0000in these cells, showed a decrease of this indicator in the studied\u0000groups with increasing duration of preservation (94.3% and 56.8%\u0000after 4 and 10 days, respectively). Based on the results of the\u0000study, it is suggested that the values of endothelial cells swelling\u0000in a potassium environment can serve as indicators of the cells’\u0000ability to restore electrogenic transport. It is concluded that\u0000the study of cell swelling in a medium with a high content of potassium\u0000ions can provide information for predicting the functionality of\u0000the graft.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"78 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disturbances of the Process of Synaptic Elimination and Its Reflection in the Wave N170 of Auditory Evoked Potential (AEP) in First Psychotic Episode of Paranoid Schizophrenia","authors":"A. Yu. Arkhipov, G. I. Rodionov, V. B. Strelets","doi":"10.1134/s0022093024040276","DOIUrl":"https://doi.org/10.1134/s0022093024040276","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In this work the response of cortical structures to auditory\u0000stimuli of different emotional significance (verbal stimuli associated\u0000with delusions and hallucinations and neutral ones) was investigated\u0000in healthy controls (<i>n</i> = 15;\u00007 males, 8 females) and in patients with paranoid schizophrenia\u0000(<i>n</i> = 24; 11 males, 13 females)\u0000in N170 component of the auditory evoked potential (AEP), participants\u0000of both groups had higher or specialized secondary education and\u0000nearly equal level of social well-being. In norm, an amplitude increase\u0000and latency shortening of N170 wave in the right central-parietal\u0000region for emotionally significant stimuli was found, which means\u0000the greater level of cortical activation for this type of stimuli.\u0000Along with the general decrease in cortical activation peculiar\u0000to paranoid schizophrenia patients, which is well known in scientific\u0000literature in this ERP component, our study revealed a simultaneous\u0000increase in both ERP parameters- amplitude and latency in frontal\u0000areas and their decrease in central and temporal areas- a phenomenon\u0000that we called the paradox effect (PE). PE in patients develops\u0000in response to emotionally significant verbal stimuli, which in\u0000norm cause increased activation. Thus, the study confirms our hypothesis\u0000that PEs are found on auditory ERPs as well as on visual ERPs, which\u0000we found earlier in previous studies. The fact that PE is observed\u0000at the N170 wave of the auditory evoked potential (AEP), i.e., at\u0000the same second perceptual stage as in the visual (VEP), may indicate that\u0000this response appears to be general principle for all modalities.\u0000However, because the auditory stimulus is shorter and more compact\u0000than the image, the response is earlier. In this regard, the PE\u0000to the auditory stimulus develops on N170 wave, whereas in visual\u0000modality it develops on later P200 component. Thus, in schizophrenia,\u0000a “conflict” between excitation and inhibition is detected already\u0000by 170 ms after stimulus presentation in response to significant\u0000stimuli, resulting in the PEs development. The identity of PEs detected\u0000in schizophrenia on VEP and AEP is also evidenced by the data on\u0000the similar character of cortical excitation with simultaneous increase\u0000or decrease of both N170 parameters. It is supposed that the revealed\u0000violation reflects the pathological process of synapse elimination\u0000peculiar to schizophrenia, which results in an excess of synapses\u0000in frontal areas combined with their deficit in central cortical\u0000areas.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"53 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spontaneous Tonic Activity Revealed in Rat Soleus Muscle by CLP290, a Novel Spinal Cord Potassium-Chloride Cotransporter Activator, during Hindlimb Suspension","authors":"V. E. Kalashnikov, K. V. Sergeeva, O. V. Turtikova, S. A. Tyganov, T. M. Mirzoev, B. S. Shenkman","doi":"10.1134/s002209302404032x","DOIUrl":"https://doi.org/10.1134/s002209302404032x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The electromyographic activity of the soleus muscle is a reliable\u0000indicator of its functional status. Support unloading causes an\u0000immediate cessation of electrical activity of the soleus muscle,\u0000which resumes upon restoration of the support load. Prolonged support\u0000unloading, however, results in the emergence of spontaneous electrical\u0000activity of the soleus muscle. Previous research has established\u0000a correlation between this activity and the presence of the potassium-chloride\u0000cotransporter (KCC2) on the membranes of spinal cord motor neurons.\u0000It has also been demonstrated that the administration of the KCC2\u0000activator prochlorperazine can eliminate spontaneous soleus muscle\u0000activity. Here, we aimed to investigate the effect of CLP290, an\u0000alternative KCC2 activator, on the spontaneous tonic activity of the\u0000rat soleus muscle. The results indicated that daily administration\u0000of CLP290 to rats during a 14-day period of hindlimb suspension\u0000prevented the reduction in KCC2 levels in lumbar spinal cord motor neurons\u0000and the increase in soleus muscle spontaneous tonic activity. Notably,\u0000there were no significant differences in the cross-sectional area\u0000of slow-type fibers between the antiorthostatic suspension groups with\u0000and without CLP290 administration.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"41 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}