L. E. Katkova, G. S. Baturina, M. M. Teterin, A. I. Sakhanenko, I. G. Palchikova, I. A. Iskakov, E. I. Solenov
{"title":"Effect of Preservation on Changes in the Volume of Corneal Endothelial Cells in an Environment with a High Concentration of Potassium","authors":"L. E. Katkova, G. S. Baturina, M. M. Teterin, A. I. Sakhanenko, I. G. Palchikova, I. A. Iskakov, E. I. Solenov","doi":"10.1134/s0022093024040331","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The experimental study of preservation length on the effect\nof high potassium concentration in the medium on the volume of human\ncorneal endothelial cells was done. The results of the study of individual\nsamples of fragments of donor material and the values calculated\nusing joined data after hypothermic preservation for 4 and 10 days\nare presented. The increase of the time when cornea samples are\nkept in preservation medium (Eusol-C) at 4°C led to a decrease in\nthe average value (<i>M</i> ± <i>SEM</i>) of cell swelling indicator (<i>Q</i>) in a potassium medium from 1.055\n± 0.001; <i>n</i> = 982 to 1.014 ±\n0.001; <i>n</i> = 338; after 4 and\n10 days, respectively. Student’s <i>t-</i>test\nfor independent samples showed a high degree of significance for\nthe difference between these values (<i>p</i> =\n2E-76). Identification of the proportion of cells capable to swell\nin a medium with a high content of potassium ions (<i>Q</i> ≥ 1), reflecting the electrogenic activity\nin these cells, showed a decrease of this indicator in the studied\ngroups with increasing duration of preservation (94.3% and 56.8%\nafter 4 and 10 days, respectively). Based on the results of the\nstudy, it is suggested that the values of endothelial cells swelling\nin a potassium environment can serve as indicators of the cells’\nability to restore electrogenic transport. It is concluded that\nthe study of cell swelling in a medium with a high content of potassium\nions can provide information for predicting the functionality of\nthe graft.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evolutionary Biochemistry and Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024040331","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The experimental study of preservation length on the effect
of high potassium concentration in the medium on the volume of human
corneal endothelial cells was done. The results of the study of individual
samples of fragments of donor material and the values calculated
using joined data after hypothermic preservation for 4 and 10 days
are presented. The increase of the time when cornea samples are
kept in preservation medium (Eusol-C) at 4°C led to a decrease in
the average value (M ± SEM) of cell swelling indicator (Q) in a potassium medium from 1.055
± 0.001; n = 982 to 1.014 ±
0.001; n = 338; after 4 and
10 days, respectively. Student’s t-test
for independent samples showed a high degree of significance for
the difference between these values (p =
2E-76). Identification of the proportion of cells capable to swell
in a medium with a high content of potassium ions (Q ≥ 1), reflecting the electrogenic activity
in these cells, showed a decrease of this indicator in the studied
groups with increasing duration of preservation (94.3% and 56.8%
after 4 and 10 days, respectively). Based on the results of the
study, it is suggested that the values of endothelial cells swelling
in a potassium environment can serve as indicators of the cells’
ability to restore electrogenic transport. It is concluded that
the study of cell swelling in a medium with a high content of potassium
ions can provide information for predicting the functionality of
the graft.
期刊介绍：
Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.
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