Journal of Evolutionary Biochemistry and Physiology最新文献

{"title":"Role of the Mast Cell–Neuron Tandem in Cardiac Function Regulation in Cardiovascular Pathologies","authors":"M. P. Morozova, A. D. Kurenkova, B. A. Umarova","doi":"10.1134/s0022093024020145","DOIUrl":"https://doi.org/10.1134/s0022093024020145","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The review addresses new data on the involvement of mast cells\u0000(MCs) in the regulation of cardiac function during the development\u0000of some cardiovascular pathologies. Under normal conditions, the number\u0000of MCs in the heart is very small compared to their abundance in\u0000barrier tissues. They provide cardiac tissue homeostasis and the\u0000life-long interaction of the heart with the nervous and endocrine systems.\u0000Cardiac pathology is accompanied by the development of the inflammatory\u0000response, with MCs playing a significant role at all stages of this\u0000process. The number of MCs significantly increases as myocardial\u0000infarction, cardiac fibrosis and coronary atherosclerosis develop.\u0000Meanwhile, their pathogenic contribution to cardiovascular pathologies\u0000is ambiguous because, on the one hand, it is aimed at cardiac tissue\u0000adaptation to injury, while on the other hand, the avalanche-like\u0000synthesis and release of mediators from activated MCs exacerbates\u0000the clinical course of the process. As a result, MCs change their\u0000secretory profile, interfere in the realization of cardiac functions\u0000against the background of inflammation, but at the same time modulate\u0000the afferent information flow from the heart and efferent influences\u0000of the nervous system. The coordinated activity of the system loses\u0000its stability, leading as a rule to severe consequences for the\u0000whole organism. The current status quo analysis of the problem indicates\u0000that cardiac activity depends on the functional state of MCs and\u0000their complex interaction with the nervous system both under normal\u0000conditions and, especially, against the background of developing pathological\u0000processes.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"148 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140886717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulation of the Spinal Cord of Decerebrated Rat with Double Pulses","authors":"V. A. Lyakhovetskii, P. Yu. Shkorbatova, O. V. Gorsky, N. V. Pavlova, E. Yu. Bazhenova, P. E. Musienko, N. S. Merkulyeva","doi":"10.1134/s0022093024020054","DOIUrl":"https://doi.org/10.1134/s0022093024020054","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Analysis of responses on electrical stimulation is one of\u0000the experimental paradigms to study the excitability of the nervous\u0000system. In particular, the technique of recording muscle responses\u0000evoked by electrical epidural stimulation (ES) of the spinal cord\u0000(SC) in humans and animals is widely used. In rats decerebrated\u0000at the precollicular level, responses of <i>mm.\u0000tibialis anterior</i> (TA) and <i>gastrocnemius medialis</i> (GM)\u0000on ES of the L2, L4, L6 spinal segments and transvertebral stimulation\u0000(TS) of the VL2, VL4, VL6 vertebrae with single and double pulses\u0000were analyzed. The currents at which the amplitude of the sensory\u0000component of the response for a single pulse and one of the pulses\u0000of the pair was maximum were determined. At the minimum of these\u0000currents, the ratio of the amplitudes of the sensory component of\u0000the response to the first and second pulses to the amplitude of\u0000the sensory component of the response to a single pulse was analyzed.\u0000For both muscles, a weakening of the response to both pulses of\u0000the pair was obtained with TS VL2 and VL4, while when stimulating\u0000VL2, the TA response to the second pulse was lower than to the first.\u0000On the contrary, with ES of all segments of interest, a facilitation of\u0000the response to the second pulse was obtained for both muscles.\u0000A similar facilitation was qualitatively observed for two other\u0000muscles, <i>mm. iliacus</i> and <i>vastus lateralis</i>. Thus, the use of\u0000double pulses during stimulation made it possible to identify the\u0000dependence of the response of SC neural networks on the method of\u0000their activation (TS or ES). The facilitation of the response to\u0000the second pulse during ES is presumably explained by a decrease\u0000in presynaptic inhibition due to decerebration.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"90 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygen-Dependent Aspects of Asprosin Action","authors":"V. V. Zinchuk, J. S. O. Al-Jebur","doi":"10.1134/s0022093024020297","DOIUrl":"https://doi.org/10.1134/s0022093024020297","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Asprosin, a novel adipokine secreted mainly by white adipose\u0000tissue, regulates organismal responses to short-term fasting, initiates\u0000glucose synthesis and/or release from the liver and thus stimulates\u0000appetite, increases total energy reserves in adipose tissue. Determining\u0000the bioenergetic status of the organism, this hormone influences\u0000tissue oxygen content and, accordingly, oxygen-transporting function\u0000of blood in the case of overweight and insulin resistance. Individuals\u0000with an increased asprosin content associated with overweight and\u0000class 1 obesity, as well as insulin resistance, demonstrate a shift\u0000of the oxygen–hemoglobin dissociation curve, accompanied by an increase\u0000in the blood concentration of nitrogen monoxide gasotransmitter\u0000and a decrease in hydrogen sulfide levels. Asprosin is involved\u0000in the regulation of the gasotransmitter system and shaping the\u0000mechanisms for blood oxygen transport. Asprosin contribution to\u0000the formation oxygen supply and adaptive reserves in individuals\u0000with metabolic disorders reflects a new aspect of the mechanism\u0000of its action.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"36 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140806412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activated Endothelium Stimulates the Activity of Multipotent Mesenchymal Stromal Cells under Physiological Hypoxia or Short-Term Hypoxic Stress in vitro","authors":"O. V. Zhidkova, E. R. Andreeva, L. B. Buravkova","doi":"10.1134/s0022093024020194","DOIUrl":"https://doi.org/10.1134/s0022093024020194","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Multipotent mesenchymal stromal cells (MSCs) are used for\u0000supplementary therapy of ischemic and inflammatory diseases. After\u0000systemic administration, transmigration of MSCs to target tissues\u0000is accompanied by their interaction with activated endothelial cells\u0000(ECs) at the site of injury. In this study, we investigated the\u0000effects of TNF-α-activated ECs on MSC functions under different\u0000levels of hypoxia. For this purpose, the short-term direct co-culture\u0000of MSCs and activated TNF-α endothelial cells was used. MSCs retained\u0000their stromal phenotype and multilineage differentiation potential\u0000after the interaction with activated ECs. At the same time, there\u0000were detected changes in the expression of molecules involved in\u0000MSC contacts with other cells and the extracellular matrix (ECM),\u0000as well as in regulating ECM degradation and cell migration. Furthermore,\u0000conditioned medium from activated endothelium stimulated targeted\u0000and untargeted migration of MSCs. The interaction of MSCs with activated\u0000ECs enhanced the paracrine activity of both cell types due to upregulated\u0000transcription and elevated levels of pleiotropic cytokines IL-6\u0000and IL-8, and altered the protease–antiprotease balance. These data\u0000suggest that short-term interaction of MSCs with activated ECs may\u0000play an important role in tissue reparative and remodeling processes.\u0000Specifically, it may promote the migratory phenotype of MSCs. In\u0000contrast to physiological hypoxia (5% O₂),\u0000acute hypoxic stress (0.1% O₂, 24 h) attenuated\u0000the stimulatory effects of ECs on MSCs.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"3 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140886281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Titin Phosphorylation in Myocardial Stiffness Changes during Cardiomyopathies","authors":"G. Z. Mikhailova, I. M. Vikhlyantsev, V. L. Lakomkin","doi":"10.1134/s0022093024020157","DOIUrl":"https://doi.org/10.1134/s0022093024020157","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The review provides a brief analysis of the current knowledge\u0000of such a post-translational modification of titin as phosphorylation,\u0000with a focus on changes that occur during the development of heart\u0000diseases. Studies using animal models of heart diseases and cardiac\u0000biopsy from patients with various pathologies reveal changes in\u0000the level of titin phosphorylation compared to healthy controls.\u0000The development of cardiac pathology is typically accompanied by\u0000hyperphosphorylation of the S11878 site and hypophosphorylation\u0000of the S12022 site in the titin PEVK region, as well as changes\u0000in the level of site phosphorylation in the titin N2B region. The\u0000cooperative functional effect of these changes is an increase in\u0000the stiffness of cardiomyocytes and cardiac muscle as a whole, based\u0000on the viscoelastic properties of titin. Changes in the latter,\u0000in turn, result from hypo- or hyperphosphorylation of certain titin\u0000sites. The review also addresses a number of therapeutic approaches\u0000aimed at modifying titin phosphorylation levels as a means to manage\u0000viscoelastic properties of the pathological myocardium in order\u0000to normalize its contractility.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140886373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zebrafish As a Promising Experimental Model of Traumatic Brain Injury","authors":"A. D. Shevlyakov, N. P. Ilyin, D. S. Galstyan, A. N. Ikrin, T. O. Kolesnikova, K. V. Apukhtin, M. M. Kotova, V. S. Nikitin, T. G. Amstislavskaya, A. V. Kalueff","doi":"10.1134/s0022093024020133","DOIUrl":"https://doi.org/10.1134/s0022093024020133","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Traumatic brain injury (TBI) involves multiple types of physical\u0000brain lesions. TBI is a highly heterogeneous clinical condition\u0000whose symptoms include cognitive, motor and emotional deficits,\u0000as well as neurodegeneration and neuroinflammation. TBI modeling\u0000in animals plays a key role in studying its pathogenetic mechanisms\u0000and temporal dynamics, as well as developing new drugs for its treatment. Recently,\u0000the use of the zebrafish <i>Danio rerio</i> as\u0000a model organism has aroused the growing interest of translational\u0000neurobiology. Zebrafish are now the second most demanded laboratory\u0000animal species in biomedicine, after mice. Here, we discuss the\u0000prospects of using this teleost fish as a model of TBI, as well\u0000as new research trends in this area. We also emphasize the importance\u0000of zebrafish as a highly translational model for studying the molecular\u0000mechanisms and neurological disorders in TBI, as well as screening\u0000for potential therapeutic agents.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"52 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Molecules Characterizing Metabolic and Cytotoxic Activity of Different Natural Killer Cell Subpopulations in Peripheral Blood during Pregnancy","authors":"E. G. Orlova, O. A. Loginova, O. L. Gorbunova, S. V. Shirshev","doi":"10.1134/s0022093024020248","DOIUrl":"https://doi.org/10.1134/s0022093024020248","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The functions of peripheral blood natural killer (NK) cells\u0000change significantly throughout pregnancy, which is mainly due to\u0000the inhibition of their cytotoxicity. Although the functional activity\u0000of NK cells is directly related to their metabolic status, these\u0000changes during physiological pregnancy have not been studied. The\u0000aim of this work was to study the expression of Glut-1, CD94, and\u0000CD107a molecules characterizing metabolic and cytotoxic activity,\u0000as well as the mitochondrial mass, in different peripheral blood\u0000NK cell subpopulations of healthy women during first and third trimesters\u0000of physiological pregnancy. The control group comprised healthy\u0000non-pregnant women in the follicular phase of the menstrual cycle.\u0000The expression of Glut-1, CD94, CD107a molecules and the mitochondrial\u0000mass were analyzed by flow cytometry in regulatory (CD16^–CD56^bright),\u0000cytotoxic (CD16⁺CD56^dim),\u0000and minor cytotoxic (CD16^hiCD56^–)\u0000NK cells. It was found that in non-pregnant women, minor cytotoxic CD16^hiCD56^– NK\u0000cells had the highest expression of Glut-1 and CD107a and the lowest\u0000expression of CD94 compared to other NK cell subpopulations. In\u0000regulatory CD16^–CD56^bright and\u0000cytotoxic CD16⁺CD56^dim NK\u0000cells, the expression of these molecules was similar, as was the\u0000mitochondrial mass in all subpopulations studied. In the first trimester,\u0000Glut-1 expression increased on regulatory CD16^–CD56^bright NK\u0000cells, while mitochondrial mass and the expression of CD94 and CD107a\u0000on all NK cells did not differ from those in non-pregnant women.\u0000In the third trimester, mitochondrial mass increased, but CD94 expression\u0000decreased, in cytotoxic CD16⁺CD56^dim NK\u0000cells compared to non-pregnant women, while CD94 expression on regulatory\u0000CD16^–CD56^bright NK\u0000cells was higher than in the first trimester. CD107a expression\u0000on minor cytotoxic CD16^hiCD56^– NK\u0000cells decreased, while remaining unchanged in other subpopulations\u0000compared to non-pregnant women. Glut-1 expression did not change\u0000in all subpopulations. Thus, different subpopulations of peripheral\u0000blood NK cells are heterogeneous in the expression of Glut-1, CD107a,\u0000and CD94. The expression of these molecules during physiological\u0000pregnancy varies by trimesters. The obtained results are important\u0000for understanding the regulatory mechanisms of NK cell functioning\u0000during pregnancy.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"19 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140806413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time Scale of Adaptation at the Tonal Sequence Processing in the Awake Mice Auditory Cortex Neurons","authors":"M. A. Egorova, A. G. Akimov, G. D. Khorunzhii","doi":"10.1134/s0022093024010241","DOIUrl":"https://doi.org/10.1134/s0022093024010241","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The study was first carried out on stimulus-specific adaptation\u0000of neurons in the primary and anterior fields of the awake house\u0000mice auditory cortex to sound sequences of four 100-ms tonal signals,\u0000with a frequency of tones corresponding to the neuronal characteristic\u0000frequency, and also with the inter-tone interval constant for one\u0000sequence and varying from 0 to 2000 ms in different sequences. The\u0000analysis of the data obtained showed the adaptation effect in the\u0000responses of all studied primary auditory cortex neurons, which\u0000was observed as the absence or significant decrease in activity\u0000evoked by the components of a series of tones following the 1st,\u0000at inter-stimulus intervals of 0–500 ms. A quantitative assessment\u0000of the adaptation effects as a function of inter-stimulus intervals\u0000within the tonal sequence, performed over a whole population of\u0000studied neurons, showed that the individual time scales of adaptation\u0000of neurons varied significantly, which may be crucial for the formation\u0000of optimal time windows for the processing of grouping and separation\u0000of sound events, which are important both for perception of animal vocalizations\u0000and human speech.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"26 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Cooperation on the Event-Related Potentials in Verbal Creative and Noncreative Tasks Performance","authors":"Zh. V. Nagornova, N. V. Shemyakina","doi":"10.1134/s0022093024010083","DOIUrl":"https://doi.org/10.1134/s0022093024010083","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>This study examines the effects of social interaction conditions\u0000on brain correlates of verbal creativity. A model of cooperation\u0000is considered in the study in which participants were to work together\u0000without conversation and create as many answers as possible in creative\u0000and noncreative tasks. Subjects (4 males, 26 female) performed two\u0000types of tasks individually and cooperatively in pairs (male-male,\u0000female-female): creative task—to create as many as possible unusual\u0000use of an everyday object, and noncreative task—to list items from\u0000the proposed categories. Event-related potentials (ERPs) in each\u0000of the tasks were compared between the cooperative and individual\u0000performance conditions. The cooperative conditions demonstrated\u0000decrease in the amplitude of the P200 component at a time interval\u0000of 148–272 ms from the onset of stimulus presentation for both creative\u0000and noncreative tasks performance, reflecting reduced attention\u0000allocation to the stimulus in the social interaction condition.\u0000When performing a creative task, a larger amplitude of the late\u0000positive component was observed in the 662–1240 ms interval in the\u0000posterior temporal and occipital areas, which may reflect activation\u0000of the temporoparietal junction involved in the processes of understanding\u0000the other. The number of answers (productivity) was significantly\u0000lower in cooperative conditions compared to individual task performance.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"10 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methods for Assessing Left Ventricular Diastolic Distensibility","authors":"V. I. Kapelko, V. L. Lakomkin, A. A. Abramov, A. V. Prosvirnin","doi":"10.1134/s0022093024010290","DOIUrl":"https://doi.org/10.1134/s0022093024010290","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The major property of the myocardium that determines left\u0000ventricular (LV) filling is its distensibility. The simplest measure\u0000for its assessment is the LV end-diastolic pressure–volume ratio,\u0000although it can vary within a wide range and is highly dependent\u0000on inflow and resistance boundary conditions, making distensibility\u0000difficult to assess. Here, we consider six calculated indices of\u0000LV diastolic stiffness, most of which are based on Hooke’s law,\u0000comparing their stability, variation, and correlation coefficients\u0000with different hemodynamic parameters. The diastolic stiffness index\u0000No. 4 proved to be the only measure that takes into account an increase\u0000in LV stiffness over diastole. It shows a weak dependence on LV ejection\u0000fraction, heart rate, and other parameters of LV hemodynamics, and\u0000hence can be used to assess LV diastolic distensibility in various\u0000cardiac pathologies.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"2 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140148134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}