V. A. Lyakhovetskii, P. Yu. Shkorbatova, O. V. Gorsky, N. V. Pavlova, E. Yu. Bazhenova, P. E. Musienko, N. S. Merkulyeva
{"title":"Stimulation of the Spinal Cord of Decerebrated Rat with Double Pulses","authors":"V. A. Lyakhovetskii, P. Yu. Shkorbatova, O. V. Gorsky, N. V. Pavlova, E. Yu. Bazhenova, P. E. Musienko, N. S. Merkulyeva","doi":"10.1134/s0022093024020054","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Analysis of responses on electrical stimulation is one of\nthe experimental paradigms to study the excitability of the nervous\nsystem. In particular, the technique of recording muscle responses\nevoked by electrical epidural stimulation (ES) of the spinal cord\n(SC) in humans and animals is widely used. In rats decerebrated\nat the precollicular level, responses of <i>mm.\ntibialis anterior</i> (TA) and <i>gastrocnemius medialis</i> (GM)\non ES of the L2, L4, L6 spinal segments and transvertebral stimulation\n(TS) of the VL2, VL4, VL6 vertebrae with single and double pulses\nwere analyzed. The currents at which the amplitude of the sensory\ncomponent of the response for a single pulse and one of the pulses\nof the pair was maximum were determined. At the minimum of these\ncurrents, the ratio of the amplitudes of the sensory component of\nthe response to the first and second pulses to the amplitude of\nthe sensory component of the response to a single pulse was analyzed.\nFor both muscles, a weakening of the response to both pulses of\nthe pair was obtained with TS VL2 and VL4, while when stimulating\nVL2, the TA response to the second pulse was lower than to the first.\nOn the contrary, with ES of all segments of interest, a facilitation of\nthe response to the second pulse was obtained for both muscles.\nA similar facilitation was qualitatively observed for two other\nmuscles, <i>mm. iliacus</i> and <i>vastus lateralis</i>. Thus, the use of\ndouble pulses during stimulation made it possible to identify the\ndependence of the response of SC neural networks on the method of\ntheir activation (TS or ES). The facilitation of the response to\nthe second pulse during ES is presumably explained by a decrease\nin presynaptic inhibition due to decerebration.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"90 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evolutionary Biochemistry and Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024020054","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Analysis of responses on electrical stimulation is one of
the experimental paradigms to study the excitability of the nervous
system. In particular, the technique of recording muscle responses
evoked by electrical epidural stimulation (ES) of the spinal cord
(SC) in humans and animals is widely used. In rats decerebrated
at the precollicular level, responses of mm.
tibialis anterior (TA) and gastrocnemius medialis (GM)
on ES of the L2, L4, L6 spinal segments and transvertebral stimulation
(TS) of the VL2, VL4, VL6 vertebrae with single and double pulses
were analyzed. The currents at which the amplitude of the sensory
component of the response for a single pulse and one of the pulses
of the pair was maximum were determined. At the minimum of these
currents, the ratio of the amplitudes of the sensory component of
the response to the first and second pulses to the amplitude of
the sensory component of the response to a single pulse was analyzed.
For both muscles, a weakening of the response to both pulses of
the pair was obtained with TS VL2 and VL4, while when stimulating
VL2, the TA response to the second pulse was lower than to the first.
On the contrary, with ES of all segments of interest, a facilitation of
the response to the second pulse was obtained for both muscles.
A similar facilitation was qualitatively observed for two other
muscles, mm. iliacus and vastus lateralis. Thus, the use of
double pulses during stimulation made it possible to identify the
dependence of the response of SC neural networks on the method of
their activation (TS or ES). The facilitation of the response to
the second pulse during ES is presumably explained by a decrease
in presynaptic inhibition due to decerebration.
期刊介绍：
Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.
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