Journal of Evolutionary Biochemistry and Physiology最新文献

{"title":"Tryptophan Signaling Molecules in Children with Different Developmental Dynamics of Obesity","authors":"O. P. Shatova, I. M. Kolesnikova, E. M. Yagodkina, S. S. Kaydoshko, A. M. Gaponov, S. A. Roumiantsev, A. V. Shestopalov","doi":"10.1134/s002209302404001x","DOIUrl":"https://doi.org/10.1134/s002209302404001x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The high incidence of childhood obesity is a big healthcare\u0000challenge worldwide, which is fraught with the development of not\u0000only numerous somatic comorbidities but also children’s social maladaptation. Searching\u0000for early biochemical markers of obesity stabilization and its progression\u0000at an older age is very important for identifying risk groups and\u0000preventing the development of obesity and its complications. Our\u0000study showed that the child’ sex, which is often disregarded in\u0000these situations, should necessarily be considered when assessing\u0000the developmental dynamics of obesity. Whereas in girls, a decrease\u0000in serum serotonin concentrations may serve as a marker of obesity\u0000stabilization/progression, in boys, this tendency can be predicted\u0000by reduced serum indole-3-acrylate levels. In both sexes, elevated\u0000fecal indole-3-carboxaldehyde levels can be indicative of obesity\u0000stabilization/progression.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Apamin on the Extracellularly Recorded Action Potentials Profiles of Subepicardial Cardiomyocytes of the Rat Heart in Myocardial Infarction","authors":"A. V. Stepanov, M. G. Dobretsov, Yu. A. Filippov, I. V. Kubasov","doi":"10.1134/s0022093024040057","DOIUrl":"https://doi.org/10.1134/s0022093024040057","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The role of small-conductance Ca²⁺-activated\u0000K⁺-channels (SK channels) in the pathogenesis\u0000of cardiomyopathies of various etiologies remains poorly understood.\u0000The purpose of this work was to evaluate the effect of the blocker\u0000of SK channels, apamin, on the extracellularly recorded action potentials\u0000(eAPs) of subepicardial myocytes in the left ventricles of sham-operated\u0000rats and rats with myocardial infarction caused by ischemia-reperfusion.\u0000It was found that local delivery of the SK channel blocker apamin\u0000at a concentration of 500 nM to the eAP recording area did not affect\u0000the eAP profiles in the group of sham-operated rats but caused a\u0000significant slowdown in the repolarization time and a decrease in\u0000the afterhyperpolarization phase of eAPs in the group of rats with\u0000myocardial infarction. These data suggest that changes in the waveform\u0000of eAPs after infarction are associated with increased expression\u0000and/or activity of SK channels in subepicardial myocytes. The possible\u0000role of these channels in the structural and functional remodeling\u0000of the myocardium of the left ventricle of the heart after ischemia-reperfusion\u0000is discussed.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concentration and Composition of Circulating Adipocyte-Derived Extracellular Vesicles in Patients with Colonic Polyps and Colorectal Cancer","authors":"N. V. Yunusova, D. A. Svarovsky, E. S. Kolegova, O. V. Cheremisina, D. N. Kostromitsky, I. V. Kondakova, E. A. Sidenko, A. Yu. Dobrodeev, A. E. Grigor’eva","doi":"10.1134/s0022093024040069","DOIUrl":"https://doi.org/10.1134/s0022093024040069","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Extracellular vesicles (EVs) are a heterogeneous population\u0000of membrane-bound nanoparticles (&lt; 1 µm in size) secreted by\u0000various cell types. Most of EVs circulating in human blood are derived\u0000from platelets, leukocytes, erythrocytes, and endotheliocytes. The\u0000composition of circulating adipocyte-derived EVs under various pathological\u0000conditions has been virtually unknown. Small EVs were isolated by\u0000ultrafiltration and double ultracentrifugation from blood plasma\u0000of patients with colorectal cancer (CRC) and colonic polyps with\u0000obesity or metabolic syndrome. The composition of adipocyte-derived EVs\u0000was analyzed by immunoprecipitation combined with Western blotting\u0000and flow cytometry. EVs fractions (FABP4- and CD11b-immunoprecipitated\u0000EVs, as well as EVs contained in the supernatant after removal of\u0000CD11b-positive EVs) contained a complex of adipocyte markers (FABP4,\u0000PPAR-γ, perilipin 1). In CRC patients without obesity, monocyte/macrophage-derived\u0000EVs precipitated on CD11b-coated particles were characterized by\u0000a combined overexpression of FABP4 and perilipin 1, while such an\u0000overexpression was not typical for CRC patients with metabolic syndrome\u0000or obesity. The fraction of true adipocyte-derived EVs (supernatant\u0000after removal of CD11b-positive EVs) was characterized by the presence\u0000of a complex of adipocyte markers with a predominant expression\u0000of FABP4 in all patients both with metabolic syndrome/metabolically\u0000healthy obesity and without metabolic disorders. To correctly characterize\u0000circulating EVs in patients without obesity, it is necessary first\u0000to remove the fraction of CD11b-positive monocyte/macrophage-derived\u0000EVs from EV preparations by immunoprecipitation or similar methods,\u0000and then, after removal/sorption of precipitated EVs, to analyze\u0000the composition of adipocyte-derived EVs in the supernatant using\u0000a set of above-mentioned adipocyte markers. Moreover, in patients\u0000with metabolic disorders, given the insignificant FABP4 expression\u0000in CD11b-immunoprecipitated EVs, the pre-depletion of EV preparations\u0000does not appear to be that necessary.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Analysis of Erythrocyte Osmotic Fragility across Vertebrate Taxa","authors":"B. A. Gerda, E. A. Skverchinskaya, A. Yu. Andreeva, A. A. Volkova, S. Gambaryan, I. V. Mindukshev","doi":"10.1134/s0022093024040094","DOIUrl":"https://doi.org/10.1134/s0022093024040094","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The osmotic fragility of erythrocytes is a parameter that\u0000reflects the ability of cells to endure variations in the osmotic\u0000environment. The impairment of this ability is often associated\u0000with a variety of pathologies, encompassing hemolytic anemias, malignant\u0000tumors, and cardiovascular dysfunctions. Osmotic fragility exhibits\u0000variability across different animal taxa and is closely related\u0000with ecosystems. We developed the method for assessing osmotic fragility\u0000using a laser particle size analyzer, which facilitates real-time\u0000kinetic monitoring of cell concentration changes under controlled\u0000temperature conditions. The species examined included <i>Homo sapiens</i>, <i>Rattus\u0000norvegicus domestica</i>, <i>Coturnix japonica\u0000domestica</i>, <i>Rana ridibunda</i>, <i>Carassius carassius</i>, and <i>Lampetra fluviatilis</i>. The methodology\u0000was presented in two variants: (1) manual water additions and (2)\u0000automated medium dilution. The key parameters characterizing osmotic\u0000fragility included <i>H</i>\u0000₅₀ (the\u0000osmolality that lysed half of lysis-susceptible cells), <i>H</i>\u0000₉₀ (90% cell\u0000lysis), and <i>W</i> (population heterogeneity\u0000by the degree of lysis resistance). In terms of <i>H</i>\u0000₅₀ and <i>W</i>, the results obtained via this\u0000method did not differ significantly from those obtained by spectrophotometry\u0000and flow cytometry. No significant differences were also observed between\u0000the outcomes of automated and manual variants of the method. Erythrocytes\u0000of aquatic and semiaquatic animals exhibited significantly higher\u0000resistance to hypotonic lysis. Among all species examined, amphibian\u0000(<i>Rana ridibunda</i>) and lamprey\u0000(<i>Lampetra fluviatilis</i>) erythrocytes\u0000demonstrated the lowest osmotic fragility. The most pronounced variability\u0000in the degree of lysis resistance was detected among amphibians,\u0000with an almost twofold difference compared to other taxa examined.\u0000While mammalian (human and rat) erythrocytes exhibited similar fragility\u0000levels, they were more variable in their resistance profiles. Avian\u0000erythrocytes demonstrated a half-lysis occurrence at higher osmolality levels\u0000compared to mammalian erythrocytes, although in the domestic quail\u0000(<i>Coturnix japonica domestica</i>),\u0000erythrocytes lysed over a considerably wider osmotic range and contained\u0000a subset of cells resistant to hypotonic lysis. These findings indicate\u0000that erythrocytes of lower vertebrates share a lower osmotic fragility\u0000compared to those of higher vertebrates, a phenomenon most likely\u0000attributable to embryonic characteristics, ecto-/endothermy, and\u0000habitat considerations.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Levels of Cortisol and Testosterone in American Mink (Neogale Vison) Selected for Aggressive and Tame Behavior towards Humans","authors":"E. Panova, S. Kalinina, V. Ilyukha, O. Trapezov, O. Balan, M. Nekrasova, M. Stepanova","doi":"10.1134/s0022093024040136","DOIUrl":"https://doi.org/10.1134/s0022093024040136","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Several molecules, including glucocorticoids (cortisol, corticosterone,\u0000etc.) and testosterone in mammals, are thought to be associated\u0000with tame/aggressive behaviour towards humans, but these relationships\u0000remain controversial. The American mink (<i>Neogale\u0000vison</i>) represents a model in the studies of physiological\u0000traits in aggressive and tame behavioral phenotypes. Given the seasonality\u0000in reproduction, fur moulting, and hormonal levels during the life\u0000cycle of mink and the reciprocal relationships between the hypothalamic–pituitary–adrenal\u0000and hypothalamic–pituitary–gonadal axis, it is necessary to study\u0000the levels of cortisol and testosterone in the behavioral phenotypes\u0000of the animals in various seasons. Of particular interest is the\u0000period after the breeding season (late March—early April), when\u0000the level of androgens decreases. The aim of the study was to characterize\u0000the neuroendocrine physiological phenotypes associated with aggressive\u0000and tame behaviors by measuring the levels of cortisol (basal level)\u0000and testosterone in the blood serum of American mink at the end\u0000of the breeding season. Blood samples were taken from animals selectively\u0000bred for tame and aggressive defensive reaction towards humans at\u0000the Institute of Cytology and Genetics in Novosibirsk, Russia. No differences\u0000were found in serum levels of cortisol and testosterone between\u0000aggressive and tame mink. We found a significant negative relationship\u0000between the studied hormones in tame mink. Thus, selection of American\u0000mink for either aggressive or emotionally positive responses to\u0000humans does not appear to be associated with changes in the levels\u0000of the hormones studied at the end of the breeding season.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peculiarities of Brain Cell Functioning in Hyperglycemia and Diabetes Mellitus","authors":"M. P. Morozova, I. G. Savinkova, L. R. Gorbacheva","doi":"10.1134/s0022093024040215","DOIUrl":"https://doi.org/10.1134/s0022093024040215","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Hyperglycemia is a symptom and damaging factor of diabetes\u0000mellitus (DM) that leads to systemic complications in the body,\u0000including cerebral macro- and microangiopathies, impaired blood\u0000supply, appearance of neurodegeneration foci, and triggering neuroinflammation.\u0000Nervous tissue is characterized by a high level of energy consumption\u0000and is highly sensitive to fluctuations in the level of metabolic\u0000substrates. Therefore, it is extremely important to study the effect\u0000of high glucose levels on the functional state of the central nervous\u0000system. This review attempts to comprehensively assess the effects of\u0000hyperglycemia on brain cells. Analysis of experimental data on the\u0000morphofunctional state of neurons, microglia and astrocytes, obtained\u0000in in vivo and in vitro models of diabetes, showed that direct and indirect\u0000effects of high glucose concentrations depend on the cell type.\u0000Astrocytic and microglial receptors and intracellular signaling\u0000cascades, which mediate the effects of hyperglycemia and the development\u0000of neuroinflammation, can act as therapeutic targets for correcting\u0000diabetic consequences. Thus, the search for new ways to modulate\u0000the functional activity of glial cells may be an effective strategy\u0000in reducing the severity of CNS injuries and their aftereffects.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Apoptosis-Related Proteins P53 and Bcl-2 in the Pathogenesis of Nervous System Diseases","authors":"E. D. Bazhanova, A. A. Kozlov","doi":"10.1134/s0022093024040173","DOIUrl":"https://doi.org/10.1134/s0022093024040173","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Diseases of the central nervous system occupy a leading place\u0000along with cardiovascular and oncological diseases, and the proportion\u0000of patients suffering from them is increasing as the population ages.\u0000This group of diseases encompasses acute conditions, such as ischemic\u0000stroke, and chronic multifactorial diseases, e.g., Alzheimer’s and\u0000Parkinson’s diseases, epilepsy, etc. The development of specific\u0000methods for their treatment is difficult, while the efficacy of\u0000the available drugs is quite low. Almost all brain diseases are\u0000underlain by common mechanisms, such as oxidative stress, inflammation, and\u0000neuronal death. Most often, cells die through apoptosis caused by\u0000an imbalance of pro- and anti-apoptotic factors. This review article\u0000addresses two of them, the pro-apoptotic transcription factor and tumor\u0000suppressor protein p53 and its opponent, the anti-apoptotic B-cell\u0000lymphoma 2 (Bcl-2) protein. The choice of these proteins for special\u0000consideration owes to the fact that both of them are key regulators\u0000of apoptosis and matter greatly in the pathogenesis of nervous diseases\u0000because neurons are not highly proliferative cells. The p53 protein\u0000is involved in the regulation of many genes responsible for DNA\u0000repair, apoptosis, and other biochemical intracellular processes,\u0000which is particularly important when studying neuronal pathology.\u0000Bcl-2 suppresses apoptosis in various cells, including neurons,\u0000by controlling mitochondrial membrane permeability and inhibiting\u0000caspases. In diseases, its expression can either increase, for example,\u0000in the case of malignant tumors, or decrease, as in the case of neurodegenerative\u0000processes. As has been established, p53 and Bcl-2 closely interact\u0000while regulating apoptosis, and their ratio may be an important\u0000prognostic factor. This work was aimed to assess the role of these\u0000proteins in the pathogenesis of various diseases of the nervous\u0000system, and to characterize common dynamic patterns of their expression\u0000and coexpression.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Skeletotopy of the Mammalian Lumbar Spinal Cord","authors":"P. Yu. Shkorbatova, V. A. Lyakhovetskii, A. A. Veshchitskii, N. S. Merkulyeva","doi":"10.1134/s0022093024040021","DOIUrl":"https://doi.org/10.1134/s0022093024040021","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The spinal cord is the most phylogenetically ancient part\u0000of the central nervous system. Due to a faster growth of the spinal\u0000column relative to the spinal cord in ontogenesis, some spinal segments\u0000in adult mammals are shifted rostrally relative to the eponymous\u0000vertebrae, which is called the ascent of the spinal cord. Based\u0000on the literature data, the skeletotopy of the lumbar spinal cord\u0000was compared in 17 mammalian species, while in 4 of them, it was\u0000additionally compared in newborn and adult animals. The length ratio\u0000of the L2 segment and VL2 vertebra, as well as the number of the\u0000vertebra that encloses the 29th spinal segment, were determined\u0000as measures characterizing the degree of spinal cord ascent. Based\u0000on the literature data, digital dexterity and the encephalization\u0000quotient were also determined as measures frequently used in comparative\u0000studies. It was shown that different species differ to a greater extent\u0000in the relative length of the rostral lumbar segments, whereas within\u0000the same species, newborns differ from adults in the relative length\u0000of the caudal lumbar segments. In most species, the degree of spinal\u0000cord ascent positively correlates with digital dexterity and the\u0000encephalization quotient. The above-mentioned macroanatomical characteristics\u0000of the spinal cord may be used to analyze the relationships between\u0000adaptive mechanisms in various mammalian species.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of Single-Photon Miniature Fluorescence Microscopy and Electrophysiological Recording Methods for in vivo Studying Hippocampal Neuronal Activity","authors":"A. I. Erofeev, E. K. Vinokurov, I. E. Antifeev, O. L. Vlasova, I. B. Bezprozvanny","doi":"10.1134/s0022093024040264","DOIUrl":"https://doi.org/10.1134/s0022093024040264","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The miniature single-photon fluorescent microscope (miniscope)\u0000enables the visualization of calcium activity in vivo in freely\u0000moving laboratory animals, providing the capability to track cellular\u0000activity during the investigation of memory formation, learning,\u0000sleep, and social interactions. However, the use of calcium sensors\u0000for in vivo imaging is limited by their relatively slow (millisecond-scale)\u0000kinetics, which complicates the recording of high-frequency spike\u0000activity. The integration of methods from single-photon miniature\u0000fluorescent microscopy with electrophysiological recording, which\u0000possesses microsecond resolution, represents a potential solution\u0000to this issue. Such a combination of techniques allows for the simultaneous\u0000recording of optical and electrophysiological activity in a single\u0000animal in vivo. In this study, a flexible polyimide microelectrode\u0000was developed and integrated with the gradient lens of the miniscope.\u0000The in vivo tests conducted in this research confirmed that the\u0000microelectrode combined with the gradient lens facilitates simultaneous\u0000single-photon calcium imaging and local field potential recording\u0000in the hippocampus of an adult mouse.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunofluorescent Localization of Ca2+ Sensor Proteins in Somatic Motor Muscles of the Earthworm Lumbricus terrestris","authors":"L. F. Nurullin, N. D. Almazov, E. M. Volkov","doi":"10.1134/s0022093024040045","DOIUrl":"https://doi.org/10.1134/s0022093024040045","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Immunofluorescent labeling revealed calmodulin, Ca²⁺/calmodulin-dependent\u0000protein kinases I and II, synaptotagmin II and VII, and calcineurin\u0000A in somatic motor muscles of the earthworm <i>Lumbricus terrestris</i>.\u0000These Ca²⁺ sensor proteins are detected\u0000in both synaptic and extrasynaptic regions. However, synaptotagmin\u0000II and VII, as well as calcineurin A, are mainly localized in the\u0000area of neuromuscular junctions, with the synaptic localization\u0000for synaptotagmin VII and calcineurin A being pronounced most distinctly.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}