Journal of Evolutionary Biochemistry and Physiology最新文献

{"title":"Peculiarities of Brain Cell Functioning in Hyperglycemia and Diabetes Mellitus","authors":"M. P. Morozova, I. G. Savinkova, L. R. Gorbacheva","doi":"10.1134/s0022093024040215","DOIUrl":"https://doi.org/10.1134/s0022093024040215","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Hyperglycemia is a symptom and damaging factor of diabetes\u0000mellitus (DM) that leads to systemic complications in the body,\u0000including cerebral macro- and microangiopathies, impaired blood\u0000supply, appearance of neurodegeneration foci, and triggering neuroinflammation.\u0000Nervous tissue is characterized by a high level of energy consumption\u0000and is highly sensitive to fluctuations in the level of metabolic\u0000substrates. Therefore, it is extremely important to study the effect\u0000of high glucose levels on the functional state of the central nervous\u0000system. This review attempts to comprehensively assess the effects of\u0000hyperglycemia on brain cells. Analysis of experimental data on the\u0000morphofunctional state of neurons, microglia and astrocytes, obtained\u0000in in vivo and in vitro models of diabetes, showed that direct and indirect\u0000effects of high glucose concentrations depend on the cell type.\u0000Astrocytic and microglial receptors and intracellular signaling\u0000cascades, which mediate the effects of hyperglycemia and the development\u0000of neuroinflammation, can act as therapeutic targets for correcting\u0000diabetic consequences. Thus, the search for new ways to modulate\u0000the functional activity of glial cells may be an effective strategy\u0000in reducing the severity of CNS injuries and their aftereffects.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"59 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Skeletotopy of the Mammalian Lumbar Spinal Cord","authors":"P. Yu. Shkorbatova, V. A. Lyakhovetskii, A. A. Veshchitskii, N. S. Merkulyeva","doi":"10.1134/s0022093024040021","DOIUrl":"https://doi.org/10.1134/s0022093024040021","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The spinal cord is the most phylogenetically ancient part\u0000of the central nervous system. Due to a faster growth of the spinal\u0000column relative to the spinal cord in ontogenesis, some spinal segments\u0000in adult mammals are shifted rostrally relative to the eponymous\u0000vertebrae, which is called the ascent of the spinal cord. Based\u0000on the literature data, the skeletotopy of the lumbar spinal cord\u0000was compared in 17 mammalian species, while in 4 of them, it was\u0000additionally compared in newborn and adult animals. The length ratio\u0000of the L2 segment and VL2 vertebra, as well as the number of the\u0000vertebra that encloses the 29th spinal segment, were determined\u0000as measures characterizing the degree of spinal cord ascent. Based\u0000on the literature data, digital dexterity and the encephalization\u0000quotient were also determined as measures frequently used in comparative\u0000studies. It was shown that different species differ to a greater extent\u0000in the relative length of the rostral lumbar segments, whereas within\u0000the same species, newborns differ from adults in the relative length\u0000of the caudal lumbar segments. In most species, the degree of spinal\u0000cord ascent positively correlates with digital dexterity and the\u0000encephalization quotient. The above-mentioned macroanatomical characteristics\u0000of the spinal cord may be used to analyze the relationships between\u0000adaptive mechanisms in various mammalian species.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"2 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Apoptosis-Related Proteins P53 and Bcl-2 in the Pathogenesis of Nervous System Diseases","authors":"E. D. Bazhanova, A. A. Kozlov","doi":"10.1134/s0022093024040173","DOIUrl":"https://doi.org/10.1134/s0022093024040173","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Diseases of the central nervous system occupy a leading place\u0000along with cardiovascular and oncological diseases, and the proportion\u0000of patients suffering from them is increasing as the population ages.\u0000This group of diseases encompasses acute conditions, such as ischemic\u0000stroke, and chronic multifactorial diseases, e.g., Alzheimer’s and\u0000Parkinson’s diseases, epilepsy, etc. The development of specific\u0000methods for their treatment is difficult, while the efficacy of\u0000the available drugs is quite low. Almost all brain diseases are\u0000underlain by common mechanisms, such as oxidative stress, inflammation, and\u0000neuronal death. Most often, cells die through apoptosis caused by\u0000an imbalance of pro- and anti-apoptotic factors. This review article\u0000addresses two of them, the pro-apoptotic transcription factor and tumor\u0000suppressor protein p53 and its opponent, the anti-apoptotic B-cell\u0000lymphoma 2 (Bcl-2) protein. The choice of these proteins for special\u0000consideration owes to the fact that both of them are key regulators\u0000of apoptosis and matter greatly in the pathogenesis of nervous diseases\u0000because neurons are not highly proliferative cells. The p53 protein\u0000is involved in the regulation of many genes responsible for DNA\u0000repair, apoptosis, and other biochemical intracellular processes,\u0000which is particularly important when studying neuronal pathology.\u0000Bcl-2 suppresses apoptosis in various cells, including neurons,\u0000by controlling mitochondrial membrane permeability and inhibiting\u0000caspases. In diseases, its expression can either increase, for example,\u0000in the case of malignant tumors, or decrease, as in the case of neurodegenerative\u0000processes. As has been established, p53 and Bcl-2 closely interact\u0000while regulating apoptosis, and their ratio may be an important\u0000prognostic factor. This work was aimed to assess the role of these\u0000proteins in the pathogenesis of various diseases of the nervous\u0000system, and to characterize common dynamic patterns of their expression\u0000and coexpression.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"9 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Levels of Cortisol and Testosterone in American Mink (Neogale Vison) Selected for Aggressive and Tame Behavior towards Humans","authors":"E. Panova, S. Kalinina, V. Ilyukha, O. Trapezov, O. Balan, M. Nekrasova, M. Stepanova","doi":"10.1134/s0022093024040136","DOIUrl":"https://doi.org/10.1134/s0022093024040136","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Several molecules, including glucocorticoids (cortisol, corticosterone,\u0000etc.) and testosterone in mammals, are thought to be associated\u0000with tame/aggressive behaviour towards humans, but these relationships\u0000remain controversial. The American mink (<i>Neogale\u0000vison</i>) represents a model in the studies of physiological\u0000traits in aggressive and tame behavioral phenotypes. Given the seasonality\u0000in reproduction, fur moulting, and hormonal levels during the life\u0000cycle of mink and the reciprocal relationships between the hypothalamic–pituitary–adrenal\u0000and hypothalamic–pituitary–gonadal axis, it is necessary to study\u0000the levels of cortisol and testosterone in the behavioral phenotypes\u0000of the animals in various seasons. Of particular interest is the\u0000period after the breeding season (late March—early April), when\u0000the level of androgens decreases. The aim of the study was to characterize\u0000the neuroendocrine physiological phenotypes associated with aggressive\u0000and tame behaviors by measuring the levels of cortisol (basal level)\u0000and testosterone in the blood serum of American mink at the end\u0000of the breeding season. Blood samples were taken from animals selectively\u0000bred for tame and aggressive defensive reaction towards humans at\u0000the Institute of Cytology and Genetics in Novosibirsk, Russia. No differences\u0000were found in serum levels of cortisol and testosterone between\u0000aggressive and tame mink. We found a significant negative relationship\u0000between the studied hormones in tame mink. Thus, selection of American\u0000mink for either aggressive or emotionally positive responses to\u0000humans does not appear to be associated with changes in the levels\u0000of the hormones studied at the end of the breeding season.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"58 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunofluorescent Localization of Ca2+ Sensor Proteins in Somatic Motor Muscles of the Earthworm Lumbricus terrestris","authors":"L. F. Nurullin, N. D. Almazov, E. M. Volkov","doi":"10.1134/s0022093024040045","DOIUrl":"https://doi.org/10.1134/s0022093024040045","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Immunofluorescent labeling revealed calmodulin, Ca²⁺/calmodulin-dependent\u0000protein kinases I and II, synaptotagmin II and VII, and calcineurin\u0000A in somatic motor muscles of the earthworm <i>Lumbricus terrestris</i>.\u0000These Ca²⁺ sensor proteins are detected\u0000in both synaptic and extrasynaptic regions. However, synaptotagmin\u0000II and VII, as well as calcineurin A, are mainly localized in the\u0000area of neuromuscular junctions, with the synaptic localization\u0000for synaptotagmin VII and calcineurin A being pronounced most distinctly.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"68 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadotropin-Releasing Hormone and Organs of the Immune System","authors":"I. V. Maiborodin, I. O. Marinkin, N. V. Onoprienko, V. I. Maiborodina","doi":"10.1134/s0022093024040197","DOIUrl":"https://doi.org/10.1134/s0022093024040197","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In this review, we address the physiological aspects of the\u0000gonadotropin-releasing hormone (GnRH) effects on the immune organs,\u0000such as red bone marrow, thymus, spleen, and lymph nodes. The use\u0000of GnRH drugs leads to the replacement of red bone marrow with yellow\u0000one and an increase in the content of lymphoid and myeloid progenitor\u0000cells. These processes are paralleled by osteoporosis due to increased\u0000bone resorption with changes in calcium metabolism and a decrease\u0000in the density of various bone tissues, although there are opposing\u0000reports that GnRH has no effect on bone density and calcium metabolism.\u0000GnRH affects the thymus during both embryonic development and postnatal\u0000ontogenesis, in inflammation and age-related involution. Not only\u0000does GnRH cause changes in the thymus, but the thymus may in turn\u0000influence the GnRH system. Although a direct impact of GnRH on splenic\u0000cells has not been detected, splenic mass changed due to active\u0000immunization against GnRH in experiment. Unfortunately, very few\u0000articles demonstrate the physiological mechanisms of immunomodulation\u0000in such conditions. In any case, the obvious insufficiency and inconsistency\u0000of publications on each aspect of GnRH effects indicate that they\u0000are still poorly understood, and it is worth furthering not only\u0000applied but also fundamental studies in this direction, particularly\u0000in the context of developing effective systems for immune control.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"95 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship of Hemorheological Blood Values and Blood Velocity of Microcirculatory Bloodstream in Rats’ Skin Vessels","authors":"N. N. Petrishchev, M. A. Skedina, T. G. Grishacheva, S. G. Chefu, A. A. Kovaleva, A. M. Nosovskij","doi":"10.1134/s0022093024040252","DOIUrl":"https://doi.org/10.1134/s0022093024040252","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Non-invasive study of blood rheology is relevant, but quite\u0000complex issue. When systemic blood viscosity and hematocrit levels\u0000deviate, blood flow indicators in different parts of microvasculature change.\u0000Purpose of the study—research of blood flow characteristics in skin\u0000microcirculatory bloodstream of rats obtained by high-frequency\u0000Doppler ultrasound (HFDU) with given changes in rheological blood\u0000indicators. The studies were carried out on pubescent male Wistar\u0000rats. 3 experimental groups were formed. Group 1 (<i>n</i> = 21) “Hemodilution”—viscosity\u00001.99 ± 0.02 mPa*s, hematocrit 31.48 ± 0.31%. Group 2 (<i>n</i> = 32) “Norma”—animals with unchanged\u0000blood levels—viscosity 2.84 ± 0.03 mPa*s, hematocrit 41.60 ± 0.3%.\u0000Group 3 (<i>n</i> = 32) “Erythrocytosis”—viscosity\u00003.95 ± 0.04 mPa*s, hematocrit 54.56 ± 0.23%. Dynamic blood viscosity\u0000in vitro studies were carried out on oscillatory viscometer. In\u0000order to evaluate hematocrit level heparinized whole blood was centrifuged\u0000in glass capillaries using; hematocrit values were assessed taking\u0000into account sedimentation of formed elements column using a hematocrit\u0000reader card. Blood flow in skin microcirculatory bloodstream of\u0000rats` left thigh area was estimated by HFDU method using Minimax-Doppler-K\u0000hardware and software system, with ultrasound transducer (frequency\u000020 MHz). Statistical analysis showed the models are correct. Blood\u0000indicators of the animals in three experimental groups differed\u0000statistically and significantly in terms of blood viscosity and\u0000hematocrit. Discriminant analysis was used to determine the relations between\u0000rheological blood parameters and characteristics of blood velocity\u0000in microcirculatory bloodstream which made it possible to identify\u0000the most significant characteristics of blood flow that tend to\u0000change depending on altered blood composition. These include: mean\u0000systolic velocity <i>Vas</i> (<i>p</i> &lt; 0.01), mean velocity <i>Vam</i> (<i>p</i> &lt;\u00000.001), mean volume velocity <i>Qam</i> (<i>p</i> &lt; 0.001), vascular resistance\u0000index <i>RI</i> (<i>p</i> &lt;\u00000.01) and the percentage of blood cells moving in low-speed <i>H</i>’ (<i>p</i> =\u00000.03). The reliability of selected characteristics was checked with\u0000one-way ANOVA; and their significance in determining membership\u0000in “Hemodilution”, “Norma” or “Erythrocytosis” groups according\u0000to HFDU data was confirmed. Based on this analysis classification\u0000functions were generated for non-invasive dynamic blood viscosity determination\u0000according to Doppler ultrasound data.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"10 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of Single-Photon Miniature Fluorescence Microscopy and Electrophysiological Recording Methods for in vivo Studying Hippocampal Neuronal Activity","authors":"A. I. Erofeev, E. K. Vinokurov, I. E. Antifeev, O. L. Vlasova, I. B. Bezprozvanny","doi":"10.1134/s0022093024040264","DOIUrl":"https://doi.org/10.1134/s0022093024040264","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The miniature single-photon fluorescent microscope (miniscope)\u0000enables the visualization of calcium activity in vivo in freely\u0000moving laboratory animals, providing the capability to track cellular\u0000activity during the investigation of memory formation, learning,\u0000sleep, and social interactions. However, the use of calcium sensors\u0000for in vivo imaging is limited by their relatively slow (millisecond-scale)\u0000kinetics, which complicates the recording of high-frequency spike\u0000activity. The integration of methods from single-photon miniature\u0000fluorescent microscopy with electrophysiological recording, which\u0000possesses microsecond resolution, represents a potential solution\u0000to this issue. Such a combination of techniques allows for the simultaneous\u0000recording of optical and electrophysiological activity in a single\u0000animal in vivo. In this study, a flexible polyimide microelectrode\u0000was developed and integrated with the gradient lens of the miniscope.\u0000The in vivo tests conducted in this research confirmed that the\u0000microelectrode combined with the gradient lens facilitates simultaneous\u0000single-photon calcium imaging and local field potential recording\u0000in the hippocampus of an adult mouse.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"46 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactoferrin Application for the Prevention and Restoration of Bone Tissue Alterations during Hindlimb Unloading in Rats","authors":"K. V. Gordienko, N. A. Lukicheva, A. I. Akhmetzyanova, A. K. Kolupaev, O. A. Sachenkov, T. V. Baltina, E. R. Sadchikova, G. Yu. Vassilieva","doi":"10.1134/s0022093024040306","DOIUrl":"https://doi.org/10.1134/s0022093024040306","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The effect of gravitational unloading (antiorthostatic hindlimb\u0000suspension) and subsequent recovery on the mineral density and mechanical\u0000properties of the femoral and tibial bones in Wistar rats was studied\u0000during oral administration of a human lactoferrin (LF) biotechnological\u0000analog (200 mg/kg) derived from the milk of producer goats. Bone\u0000mineral density was determined by dual energy X-ray absorptiometry,\u0000while bone strength and stiffness were assessed via the three-point\u0000bending test. It was shown that 21-day gravitational unloading led\u0000to a decrease in the mineral density of the tibia and femur. Oral\u0000LF administration had no significant effect on the mineral density\u0000and projected area of the bones. There were no statistically significant\u0000differences in mechanical stiffness between the experimental groups,\u0000although after readaptation, the ultimate strength was significantly\u0000higher in LF-administered groups. Thus, the results may indicate\u0000the potential of LF preparations as prophylactic agents for maintaining\u0000bone strength. At the same time, the maintaining of bone mineral\u0000density under deficit-stimulating conditions requires a consideration\u0000of alternative dosages and drug delivery methods.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"51 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunization of Male BALB/c and C57BL/6 Mice Alters the Composition of Their Urine and the Response of Females to Its Odor","authors":"A. S. Khotskina, Yu. V. Patrushev, D. I. Yusupova, L. A. Gerlinskaya, D. V. Petrovskii, M. P. Moshkin, E. L. Zavjalov","doi":"10.1134/s0022093024040070","DOIUrl":"https://doi.org/10.1134/s0022093024040070","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Odors play a key role in animal communication and mate choice.\u0000Male urinary odor becomes less attractive to mature females when\u0000males get infected or have their immune system activated. The development\u0000of the immune response at early stages is related to the production\u0000of both pro- and anti-inflammatory cytokines. To explore the chemical\u0000basis of the observed differences and analyze the effects of female\u0000interactions with the urinary odor of antigen-stimulated males,\u0000the males of inbred BALB/c (Th1) and C57BL/6 (Th2) mice were immunized\u0000with keyhole limpet hemocyanin (KLH). The odor of male urine collected\u00003 days after immunization proved to be less attractive to females\u0000of both strains compared to the odor of control males. Herewith,\u0000BALB/c females sniffed the urine of immunized males less frequently,\u0000while C57BL/6 females demonstrated a shorter duration of grooming\u0000in olfactory tests with urine of immunized males. The altered response\u0000of females to the odor of male urine collected on day 3 after KLH\u0000immunization matched antigen-induced modulation of relative levels\u0000of volatile organic compounds detected in urine samples by gas chromatography/mass\u0000spectroscopy (GC-MS). Males of both strains, after KLH immunization,\u0000exhibited an increase in the content of nonanal, benzothiazole, 2-<i>sec</i>-butyl-4,5-dihydrothiazole, and\u0000[1,4,5]-oxadithiepane. At the same time, no compounds were found,\u0000whose content changed multidirectionally in the urine of males after\u0000immunization in a strain-dependent manner.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"44 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}