A. S. Khotskina, Yu. V. Patrushev, D. I. Yusupova, L. A. Gerlinskaya, D. V. Petrovskii, M. P. Moshkin, E. L. Zavjalov
{"title":"Immunization of Male BALB/c and C57BL/6 Mice Alters the Composition of Their Urine and the Response of Females to Its Odor","authors":"A. S. Khotskina, Yu. V. Patrushev, D. I. Yusupova, L. A. Gerlinskaya, D. V. Petrovskii, M. P. Moshkin, E. L. Zavjalov","doi":"10.1134/s0022093024040070","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Odors play a key role in animal communication and mate choice.\nMale urinary odor becomes less attractive to mature females when\nmales get infected or have their immune system activated. The development\nof the immune response at early stages is related to the production\nof both pro- and anti-inflammatory cytokines. To explore the chemical\nbasis of the observed differences and analyze the effects of female\ninteractions with the urinary odor of antigen-stimulated males,\nthe males of inbred BALB/c (Th1) and C57BL/6 (Th2) mice were immunized\nwith keyhole limpet hemocyanin (KLH). The odor of male urine collected\n3 days after immunization proved to be less attractive to females\nof both strains compared to the odor of control males. Herewith,\nBALB/c females sniffed the urine of immunized males less frequently,\nwhile C57BL/6 females demonstrated a shorter duration of grooming\nin olfactory tests with urine of immunized males. The altered response\nof females to the odor of male urine collected on day 3 after KLH\nimmunization matched antigen-induced modulation of relative levels\nof volatile organic compounds detected in urine samples by gas chromatography/mass\nspectroscopy (GC-MS). Males of both strains, after KLH immunization,\nexhibited an increase in the content of nonanal, benzothiazole, 2-<i>sec</i>-butyl-4,5-dihydrothiazole, and\n[1,4,5]-oxadithiepane. At the same time, no compounds were found,\nwhose content changed multidirectionally in the urine of males after\nimmunization in a strain-dependent manner.</p>","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024040070","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Odors play a key role in animal communication and mate choice.
Male urinary odor becomes less attractive to mature females when
males get infected or have their immune system activated. The development
of the immune response at early stages is related to the production
of both pro- and anti-inflammatory cytokines. To explore the chemical
basis of the observed differences and analyze the effects of female
interactions with the urinary odor of antigen-stimulated males,
the males of inbred BALB/c (Th1) and C57BL/6 (Th2) mice were immunized
with keyhole limpet hemocyanin (KLH). The odor of male urine collected
3 days after immunization proved to be less attractive to females
of both strains compared to the odor of control males. Herewith,
BALB/c females sniffed the urine of immunized males less frequently,
while C57BL/6 females demonstrated a shorter duration of grooming
in olfactory tests with urine of immunized males. The altered response
of females to the odor of male urine collected on day 3 after KLH
immunization matched antigen-induced modulation of relative levels
of volatile organic compounds detected in urine samples by gas chromatography/mass
spectroscopy (GC-MS). Males of both strains, after KLH immunization,
exhibited an increase in the content of nonanal, benzothiazole, 2-sec-butyl-4,5-dihydrothiazole, and
[1,4,5]-oxadithiepane. At the same time, no compounds were found,
whose content changed multidirectionally in the urine of males after
immunization in a strain-dependent manner.
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