N. N. Petrishchev, M. A. Skedina, T. G. Grishacheva, S. G. Chefu, A. A. Kovaleva, A. M. Nosovskij
{"title":"The Relationship of Hemorheological Blood Values and Blood Velocity of Microcirculatory Bloodstream in Rats’ Skin Vessels","authors":"N. N. Petrishchev, M. A. Skedina, T. G. Grishacheva, S. G. Chefu, A. A. Kovaleva, A. M. Nosovskij","doi":"10.1134/s0022093024040252","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Non-invasive study of blood rheology is relevant, but quite\ncomplex issue. When systemic blood viscosity and hematocrit levels\ndeviate, blood flow indicators in different parts of microvasculature change.\nPurpose of the study—research of blood flow characteristics in skin\nmicrocirculatory bloodstream of rats obtained by high-frequency\nDoppler ultrasound (HFDU) with given changes in rheological blood\nindicators. The studies were carried out on pubescent male Wistar\nrats. 3 experimental groups were formed. Group 1 (<i>n</i> = 21) “Hemodilution”—viscosity\n1.99 ± 0.02 mPa*s, hematocrit 31.48 ± 0.31%. Group 2 (<i>n</i> = 32) “Norma”—animals with unchanged\nblood levels—viscosity 2.84 ± 0.03 mPa*s, hematocrit 41.60 ± 0.3%.\nGroup 3 (<i>n</i> = 32) “Erythrocytosis”—viscosity\n3.95 ± 0.04 mPa*s, hematocrit 54.56 ± 0.23%. Dynamic blood viscosity\nin vitro studies were carried out on oscillatory viscometer. In\norder to evaluate hematocrit level heparinized whole blood was centrifuged\nin glass capillaries using; hematocrit values were assessed taking\ninto account sedimentation of formed elements column using a hematocrit\nreader card. Blood flow in skin microcirculatory bloodstream of\nrats` left thigh area was estimated by HFDU method using Minimax-Doppler-K\nhardware and software system, with ultrasound transducer (frequency\n20 MHz). Statistical analysis showed the models are correct. Blood\nindicators of the animals in three experimental groups differed\nstatistically and significantly in terms of blood viscosity and\nhematocrit. Discriminant analysis was used to determine the relations between\nrheological blood parameters and characteristics of blood velocity\nin microcirculatory bloodstream which made it possible to identify\nthe most significant characteristics of blood flow that tend to\nchange depending on altered blood composition. These include: mean\nsystolic velocity <i>Vas</i> (<i>p</i> < 0.01), mean velocity <i>Vam</i> (<i>p</i> <\n0.001), mean volume velocity <i>Qam</i> (<i>p</i> < 0.001), vascular resistance\nindex <i>RI</i> (<i>p</i> <\n0.01) and the percentage of blood cells moving in low-speed <i>H</i>’ (<i>p</i> =\n0.03). The reliability of selected characteristics was checked with\none-way ANOVA; and their significance in determining membership\nin “Hemodilution”, “Norma” or “Erythrocytosis” groups according\nto HFDU data was confirmed. Based on this analysis classification\nfunctions were generated for non-invasive dynamic blood viscosity determination\naccording to Doppler ultrasound data.</p>","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024040252","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Non-invasive study of blood rheology is relevant, but quite
complex issue. When systemic blood viscosity and hematocrit levels
deviate, blood flow indicators in different parts of microvasculature change.
Purpose of the study—research of blood flow characteristics in skin
microcirculatory bloodstream of rats obtained by high-frequency
Doppler ultrasound (HFDU) with given changes in rheological blood
indicators. The studies were carried out on pubescent male Wistar
rats. 3 experimental groups were formed. Group 1 (n = 21) “Hemodilution”—viscosity
1.99 ± 0.02 mPa*s, hematocrit 31.48 ± 0.31%. Group 2 (n = 32) “Norma”—animals with unchanged
blood levels—viscosity 2.84 ± 0.03 mPa*s, hematocrit 41.60 ± 0.3%.
Group 3 (n = 32) “Erythrocytosis”—viscosity
3.95 ± 0.04 mPa*s, hematocrit 54.56 ± 0.23%. Dynamic blood viscosity
in vitro studies were carried out on oscillatory viscometer. In
order to evaluate hematocrit level heparinized whole blood was centrifuged
in glass capillaries using; hematocrit values were assessed taking
into account sedimentation of formed elements column using a hematocrit
reader card. Blood flow in skin microcirculatory bloodstream of
rats` left thigh area was estimated by HFDU method using Minimax-Doppler-K
hardware and software system, with ultrasound transducer (frequency
20 MHz). Statistical analysis showed the models are correct. Blood
indicators of the animals in three experimental groups differed
statistically and significantly in terms of blood viscosity and
hematocrit. Discriminant analysis was used to determine the relations between
rheological blood parameters and characteristics of blood velocity
in microcirculatory bloodstream which made it possible to identify
the most significant characteristics of blood flow that tend to
change depending on altered blood composition. These include: mean
systolic velocity Vas (p < 0.01), mean velocity Vam (p <
0.001), mean volume velocity Qam (p < 0.001), vascular resistance
index RI (p <
0.01) and the percentage of blood cells moving in low-speed H’ (p =
0.03). The reliability of selected characteristics was checked with
one-way ANOVA; and their significance in determining membership
in “Hemodilution”, “Norma” or “Erythrocytosis” groups according
to HFDU data was confirmed. Based on this analysis classification
functions were generated for non-invasive dynamic blood viscosity determination
according to Doppler ultrasound data.
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