Lactoferrin Application for the Prevention and Restoration of Bone Tissue Alterations during Hindlimb Unloading in Rats

Pub Date : 2024-08-29 DOI:10.1134/s0022093024040306

K. V. Gordienko, N. A. Lukicheva, A. I. Akhmetzyanova, A. K. Kolupaev, O. A. Sachenkov, T. V. Baltina, E. R. Sadchikova, G. Yu. Vassilieva

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Abstract

The effect of gravitational unloading (antiorthostatic hindlimb suspension) and subsequent recovery on the mineral density and mechanical properties of the femoral and tibial bones in Wistar rats was studied during oral administration of a human lactoferrin (LF) biotechnological analog (200 mg/kg) derived from the milk of producer goats. Bone mineral density was determined by dual energy X-ray absorptiometry, while bone strength and stiffness were assessed via the three-point bending test. It was shown that 21-day gravitational unloading led to a decrease in the mineral density of the tibia and femur. Oral LF administration had no significant effect on the mineral density and projected area of the bones. There were no statistically significant differences in mechanical stiffness between the experimental groups, although after readaptation, the ultimate strength was significantly higher in LF-administered groups. Thus, the results may indicate the potential of LF preparations as prophylactic agents for maintaining bone strength. At the same time, the maintaining of bone mineral density under deficit-stimulating conditions requires a consideration of alternative dosages and drug delivery methods.

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应用乳铁蛋白预防和恢复大鼠后肢卸力时的骨组织损伤

摘要研究了Wistar大鼠在口服从生产者山羊奶中提取的人乳铁蛋白（LF）生物技术类似物（200 mg/kg）期间，重力卸载（反静力后肢悬吊）和随后的恢复对其股骨和胫骨矿物质密度和机械性能的影响。骨矿物质密度通过双能 X 射线吸收测定法进行测定，而骨强度和硬度则通过三点弯曲试验进行评估。结果表明，21 天的重力卸载导致胫骨和股骨的矿物质密度下降。口服低密度脂蛋白对骨骼的矿物质密度和投影面积没有显著影响。实验组之间的机械刚度在统计学上没有明显差异，但在重新适应后，服用 LF 组的极限强度明显更高。因此，这些结果可能表明 LF 制剂具有作为预防性药物维持骨强度的潜力。与此同时，在缺损刺激条件下维持骨矿物质密度需要考虑其他剂量和给药方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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