{"title":"Effect of Hypernatremia on Protein Reabsorption in Renal Proximal Tubules of the Lake Frog Pelophylax ridibundus","authors":"N. P. Prutskova, E. V. Seliverstova","doi":"10.1134/s0022093024020091","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Although protein reabsorption in the renal proximal tubules\nis concurrent with ion and water transport, little is known about\nthe dependence of receptor-mediated protein endocytosis on changes\nin water and salt balance. The aim of the study was to investigate\ntubular reabsorption and intracellular vesicular transport of various\nproteins in a lake frog (<i>Pelophylax ridibundus</i>)\nmodel of hypernatremia. The frogs were injected with hypertonic\nsodium chloride solution (0.75 M NaCl) 1 h before the administration\nof green or yellow fluorescent proteins (GFP or YFP), as well as\nlysozyme. Fluorescent immunohistochemistry and confocal microscopy\nwere used to detect lysozyme and the endocytic receptor megalin\nin the epithelial cells of proximal tubules. The endocytosis dynamics\nwas assessed by automated quantification of colocalized fluorescent\nsignals of megalin and injected proteins. We found a statistically\nsignificant decrease in the reabsorption of GFP, YFP, and lysozyme\nin proximal tubules after NaCl injection. The accumulation of proteins\nin early endosomes and the decrease in their entry into late endosomes\nand lysosomes suggest a delay in the intracellular vesicular transport\nof proteins under hypernatremia. The data obtained are analyzed\nas related to changes in blood parameters and kidney activity during\nosmoregulation, as well as to the role of chloride channels in receptor-mediated\nprotein endocytosis. We assume that increased ion transport in proximal\ntubular epitheliocytes under hypernatremia conditions leads to a\ndecreased protein reabsorption capacity of these cells and delayed intracellular\ntransport of proteins.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"57 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evolutionary Biochemistry and Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024020091","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Although protein reabsorption in the renal proximal tubules
is concurrent with ion and water transport, little is known about
the dependence of receptor-mediated protein endocytosis on changes
in water and salt balance. The aim of the study was to investigate
tubular reabsorption and intracellular vesicular transport of various
proteins in a lake frog (Pelophylax ridibundus)
model of hypernatremia. The frogs were injected with hypertonic
sodium chloride solution (0.75 M NaCl) 1 h before the administration
of green or yellow fluorescent proteins (GFP or YFP), as well as
lysozyme. Fluorescent immunohistochemistry and confocal microscopy
were used to detect lysozyme and the endocytic receptor megalin
in the epithelial cells of proximal tubules. The endocytosis dynamics
was assessed by automated quantification of colocalized fluorescent
signals of megalin and injected proteins. We found a statistically
significant decrease in the reabsorption of GFP, YFP, and lysozyme
in proximal tubules after NaCl injection. The accumulation of proteins
in early endosomes and the decrease in their entry into late endosomes
and lysosomes suggest a delay in the intracellular vesicular transport
of proteins under hypernatremia. The data obtained are analyzed
as related to changes in blood parameters and kidney activity during
osmoregulation, as well as to the role of chloride channels in receptor-mediated
protein endocytosis. We assume that increased ion transport in proximal
tubular epitheliocytes under hypernatremia conditions leads to a
decreased protein reabsorption capacity of these cells and delayed intracellular
transport of proteins.
期刊介绍：
Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.
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