大鼠下丘脑小管核神经元中胰岛素信号的年龄动态变化

IF 0.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Evolutionary Biochemistry and Physiology Pub Date : 2024-04-26 DOI:10.1134/s0022093024020303

P. A. Anfimova, V. V. Porseva, L. G. Pankrasheva, E. S. Shirina, P. M. Masliukov

{"title":"大鼠下丘脑小管核神经元中胰岛素信号的年龄动态变化","authors":"P. A. Anfimova, V. V. Porseva, L. G. Pankrasheva, E. S. Shirina, P. M. Masliukov","doi":"10.1134/s0022093024020303","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The hypothalamus is a regulatory center that controls homeostasis,\nreproduction, circadian rhythms, and the endocrine system. It is\nalso involved in the regulation of aging. The expression of insulin receptors\n(INR) and insulin receptor substrate 1 (IRS1) was studied in neurons\nof the arcuate (ARN), dorsomedial (DMN), and ventromedial (VMN)\ntuberal nuclei of the rat hypothalamus in young (2 months), adult\n(12 months), and old (24 months) male Wistar rats using immunofluorescence\nstaining and Western blotting. The results showed that INR and IRS1\nexpression in neurons of the mediobasal hypothalamic nuclei changes\nin opposite directions with age. INR expression decreases in the\nARN and increases in the DMN and VMN, while IRS1 expression is unchanged\nin the ARN and VMN and decreases in the DMN.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"148 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Age-Related Dynamics of Insulin Signaling in Neurons of the Rat Hypothalamic Tuberal Nuclei\",\"authors\":\"P. A. Anfimova, V. V. Porseva, L. G. Pankrasheva, E. S. Shirina, P. M. Masliukov\",\"doi\":\"10.1134/s0022093024020303\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Abstract</h3><p>The hypothalamus is a regulatory center that controls homeostasis,\\nreproduction, circadian rhythms, and the endocrine system. It is\\nalso involved in the regulation of aging. The expression of insulin receptors\\n(INR) and insulin receptor substrate 1 (IRS1) was studied in neurons\\nof the arcuate (ARN), dorsomedial (DMN), and ventromedial (VMN)\\ntuberal nuclei of the rat hypothalamus in young (2 months), adult\\n(12 months), and old (24 months) male Wistar rats using immunofluorescence\\nstaining and Western blotting. The results showed that INR and IRS1\\nexpression in neurons of the mediobasal hypothalamic nuclei changes\\nin opposite directions with age. INR expression decreases in the\\nARN and increases in the DMN and VMN, while IRS1 expression is unchanged\\nin the ARN and VMN and decreases in the DMN.</p>\",\"PeriodicalId\":15805,\"journal\":{\"name\":\"Journal of Evolutionary Biochemistry and Physiology\",\"volume\":\"148 1\",\"pages\":\"\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2024-04-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Evolutionary Biochemistry and Physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1134/s0022093024020303\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evolutionary Biochemistry and Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024020303","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

摘要下丘脑是一个调节中枢，控制着体内平衡、生殖、昼夜节律和内分泌系统。它还参与衰老的调节。本研究采用免疫荧光和 Western 印迹技术研究了幼年（2 个月）、成年（12 个月）和老年（24 个月）雄性 Wistar 大鼠下丘脑弓状核（ARN）、背内侧核（DMN）和腹外侧核（VMN）神经元中胰岛素受体（INR）和胰岛素受体底物 1（IRS1）的表达。结果表明，INR和IRS1在下丘脑中间核神经元中的表达随着年龄的增长呈相反方向变化。INR在ARN中的表达量减少，在DMN和VMN中的表达量增加，而IRS1在ARN和VMN中的表达量不变，在DMN中的表达量减少。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Age-Related Dynamics of Insulin Signaling in Neurons of the Rat Hypothalamic Tuberal Nuclei

查看原文本刊更多论文

Age-Related Dynamics of Insulin Signaling in Neurons of the Rat Hypothalamic Tuberal Nuclei

Abstract

The hypothalamus is a regulatory center that controls homeostasis, reproduction, circadian rhythms, and the endocrine system. It is also involved in the regulation of aging. The expression of insulin receptors (INR) and insulin receptor substrate 1 (IRS1) was studied in neurons of the arcuate (ARN), dorsomedial (DMN), and ventromedial (VMN) tuberal nuclei of the rat hypothalamus in young (2 months), adult (12 months), and old (24 months) male Wistar rats using immunofluorescence staining and Western blotting. The results showed that INR and IRS1 expression in neurons of the mediobasal hypothalamic nuclei changes in opposite directions with age. INR expression decreases in the ARN and increases in the DMN and VMN, while IRS1 expression is unchanged in the ARN and VMN and decreases in the DMN.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Evolutionary Biochemistry and Physiology 生物-进化生物学

自引率

33.30%

发文量

110

审稿时长

6-12 weeks

期刊介绍： Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.