Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Newly Developed Calcium-Enriched Hemostatic Sponge Enhanced Wound Healing and Regeneration","authors":"Ika Dewi Ana,&nbsp;Gumilang Almas Pratama Satria,&nbsp;Rahmi Anggraeni,&nbsp;Any Lestari,&nbsp;Roesita Dwi Aprilia,&nbsp;Rudi Wigianto,&nbsp;Sri Yuliana,&nbsp;Yasuhiko Tabata","doi":"10.1002/jbm.b.35568","DOIUrl":"https://doi.org/10.1002/jbm.b.35568","url":null,"abstract":"<div>\u0000 \u0000 <p>This study focuses on the development, characterization, and validation of a carbonate apatite (CHA)-enriched hemostatic sponge and the evaluation of its ability to enhance wound healing and regeneration through the controlled release of calcium ions (Ca²⁺). CHA, a bioactive material resembling the natural apatite in human bone, was synthesized within gelatin matrices to form a composite sponge. The synthesis incorporated β-type carbonate ion substitutions in the CHA structure, enabling dynamic dissolution and the gradual release of Ca²⁺ ions at the site of injury. This targeted release was shown to accelerate wound healing and tissue regeneration without causing irritation. The presence of calcium ions in the CHA composite was identified as a critical factor in supporting hemostatic processes, emphasizing their importance in wound care. The CHA-enriched hemostatic sponge demonstrated superior healing outcomes, positioning it as a promising next-generation biomedical device for hemostatic purposes and wound regeneration applications. Moreover, the CHA structure offers potential for surface modifications to introduce additional functionalities, such as inherent antimicrobial properties, further enhancing its biological performance and expanding its applications in advanced wound care.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical Properties and Biocompatibility of Injectable Hydroxyapatite Cement and Its Application in Compressive Tibial Plateau Fractures","authors":"Xiaoli Zhou,&nbsp;Xiang Sun,&nbsp;Guangdong Chen,&nbsp;Yang Chen,&nbsp;Zepei Zhang,&nbsp;Zhiyong Qian,&nbsp;Qiang Zeng,&nbsp;Jun Miao","doi":"10.1002/jbm.b.35565","DOIUrl":"https://doi.org/10.1002/jbm.b.35565","url":null,"abstract":"<div>\u0000 \u0000 <p>Injectable carbonated hydroxyapatite (ICHA) cement was developed by adding 2% Hydroxypropyl methylcellulose (HPMC) to carbonated hydroxyapatite (CHA) cement, improving its rheological properties and injectability for minimally invasive orthopedic use. The cement's physical and chemical properties, including curing time, strength, porosity, and consistency, were tested in vitro. Scanning electron microscopy, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) were used to analyze the cured cement. Bone marrow stromal cells were cultured with ICHA cement extracts and specimens to test cell growth (MTT assay) and cytotoxicity. In vivo, the cement was implanted into rabbit muscles to assess inflammation and capsule formation, along with other biocompatibility tests, including hemolysis and pyrogen testing. ICHA cement sets without heat generation, with a 9-min initial setting time and a 15-min final setting time, similar to CHA cement. The strength reaches 20 MPa after 1 day and peaks at 35 MPa after 7 days. Its porosity is slightly higher than CHA cement, and it resists dilution well, preventing disintegration in water. The consistency of ICHA cement is lower than CHA cement at different time points (<i>p</i> &lt; 0.001), showing a logarithmic change pattern. With adjustable setting time, good resistance to dilution, and compressive strength similar to cancellous bone, ICHA cement is well suited for clinical use. Its composition closely resembles natural bone, offering strong fixation and stability for tibial plateau healing, which supports early movement and reduces the risk of joint stiffness and post-traumatic arthritis.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Tricalcium Phosphate Foam and Paste Bone Grafting Materials Designed for Improved Surgical Handling on Osteogenesis in a Sheep Scapula Model","authors":"Norbert Neckel,&nbsp;Jonas Wüster,&nbsp;Li Xiang-Tischhauser,&nbsp;Schugufa Mir,&nbsp;Doaa Adel-Khattab,&nbsp;Benedikt Stang,&nbsp;Andrea Kuhr,&nbsp;Dirk Barnewitz,&nbsp;Antje Genzel,&nbsp;Steffen Koerdt,&nbsp;Carsten Rendenbach,&nbsp;Max Heiland,&nbsp;Susanne Nahles,&nbsp;Michael Stiller,&nbsp;Christine Knabe","doi":"10.1002/jbm.b.35561","DOIUrl":"https://doi.org/10.1002/jbm.b.35561","url":null,"abstract":"<p>Reconstruction of critical-size bone defects (CSDs) with complex defect morphologies remains clinically challenging. The desire to avoid autograft harvesting has prompted an increasing quest for adequate synthetic bone grafting materials. The clinical success rates, which have been achieved with bioactive β-tricalcium phosphate granules (TCP-G) demonstrate that these materials have become an excellent alternative graft choice. In order to improve surgical handling properties, TCP-G have been combined with natural polymers for creating paste- and foam-like materials, which can easily be molded into any desired shape when grafting a given bony defect or deploying them with a syringe. This study assessed the effect of a TCP paste (TCP-P) and a TCP-foam (TCP-F) bone grafting material as compared to TCP-G on bone formation and osteogenic marker expression after 1, 3, 6, 12, and 18 months of implantation in CSD in the sheep scapula and tested the hypothesis that the addition of natural polymers would not diminish the osteogenic properties of TCP-P and TCP-F. The bone and bone graft material area fractions were determined histomorphometrically in order to quantify bone formation and bone graft material resorption. Immunohistochemical analysis of collagen type I, osteocalcin, and bone sialoprotein expression in the various cell and matrix components of the bone tissue was performed on resin-embedded sections for characterizing the osteogenic and bioactive properties of the test materials. By 6 months, all three TCP materials facilitated excellent defect regeneration with further bone remodeling at 12 and 18 months. TCP-F and TCP-P induced greater osteocalcin expression and exhibited more advanced graft material resorption at 1 and 6 months, respectively. At 18 months, all three grafting materials were almost fully resorbed with the original bony architecture being restored. Taken together, the hyaluronic acid and methylcellulose components in TCP-P and porcine collagen components in TCP-F did not diminish the osteogenic capacity of TCP-P and TCP-F, which exhibited an even slightly higher resorbability and enhancement effect on OC expression by osteoblasts.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35561","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piezoelectric Biomaterials for Use in Bone Tissue Engineering—A Narrative Review","authors":"Sandeep Choudhury,&nbsp;Debolina Das,&nbsp;Sandipan Roy,&nbsp;Amit Roy Chowdhury","doi":"10.1002/jbm.b.35564","DOIUrl":"https://doi.org/10.1002/jbm.b.35564","url":null,"abstract":"<div>\u0000 \u0000 <p>To examine natural bone's bioelectrical traits, notably its piezoelectricity, and to look into how these characteristics influence bone growth and repair. In the context of exploring the potential of piezoelectric biomaterials, such as biopolymers and bio-ceramics, towards orthopedic and bone regeneration applications, the research seeks to evaluate the significance of piezoelectricity-driven osteogenesis. The paper reviews recent research on bone's electrical and dielectric properties, surface polarization/electrical stimulation effects interacting with cell activity and the effectiveness of piezoelectric biomaterials to support tissues' regenerative process. The study includes a number of materials, such as collagen, polyvinylidene fluoride (PVDF) and barium titanate. The applications of piezoelectric bio-ceramics, piezoelectric organic polymers, and piezoelectric natural polymers are particularly highlighted. Piezoelectric biomaterials are being shown in recent studies to enhance cellular metabolism in vitro as well as promote the regeneration of tissues in vivo, especially when paired with electric field stimulation or interface polarization. Piezoelectric bio-ceramics like magnesium silicate and barium titanate, as well as biopolymers like collagen and PVDF, have shown possibilities for orthopedic applications. However, there are several challenges regarding the manufacturing of bio-ceramics of specific compositions having the desired properties. This review highlighted the potential of piezoelectric biomaterials in orthopedic applications with special emphasis on biopolymers and bioceramics. Therefore, these types of materials have huge potential for bone regeneration because they can mimic the piezoelectric properties of bone and allow better advances in tissue engineering or regenerative medicine. To date, little is known about their mechanism of action, and modifications are needed to improve efficacy for clinical uptake.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental Restorative Viability of Zinc Oxide Nanoparticle-Reinforced Zirconia and Baghdadite Ceramic Composites","authors":"James Martin M. Jimenez,&nbsp;Ricky Kristan M. Raguindin,&nbsp;Eduardo R. Magdaluyo Jr.","doi":"10.1002/jbm.b.35562","DOIUrl":"https://doi.org/10.1002/jbm.b.35562","url":null,"abstract":"<div>\u0000 \u0000 <p>This study explores the enhancement of properties in zirconia-based ceramic dental restorative materials through the incorporation of baghdadite (Ca₃ZrSi₂O₉) and zinc oxide (ZnO) nanoparticles. Baghdadite was synthesized via a solid-state sintering method and integrated into zirconia to form baghdadite/zirconia ceramic composites. These composites were sintered with varying concentrations of baghdadite (0%, 5%, and 10%) to enhance bioactivity and support bone tissue repair, and incorporated with 0.6% ZnO nanoparticles to improve antimicrobial properties. The crystallographic structure, surface morphology, apparent density, antibacterial properties, and cell viability were characterized using x-ray diffraction (XRD), scanning electron microscopy (SEM), Archimedes principle, agar plate method, and trypan blue exclusion assay, respectively. Protein adsorption was evaluated using bovine serum albumin (BSA). Results showed that higher concentrations of baghdadite increased protein adhesion on the surface. The agar plate method revealed that ZnO nanoparticle-reinforced zirconia/baghdadite composites exhibited significant antimicrobial activity, particularly against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. In addition, the composites demonstrated a significant high cell viability of 83.71%, promoting cell growth. These findings suggest that the incorporation of baghdadite and ZnO nanoparticles enhances the bioactivity, antimicrobial effectiveness, and biocompatibility of zirconia, making it a viable candidate for dental restorative applications.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soft Flexible Skin Conformable Nanocomposites as a Platform for Electroceutical and Orthopedic Applications","authors":"Dhruv R. Seshadri,&nbsp;Aziz N. Radwan,&nbsp;Nicholas D. Bianco,&nbsp;Joseph A. Lerchbacker,&nbsp;Audrey M. Zorman,&nbsp;Christian A. Zorman,&nbsp;Kath M. Bogie","doi":"10.1002/jbm.b.35559","DOIUrl":"https://doi.org/10.1002/jbm.b.35559","url":null,"abstract":"<p>Designing biomimetic substrates and electrodes for bioelectronic devices with the necessary mechanical, electrical, and biological properties is critical considering the potential mismatch between soft tissue and rigid electronics, where incompatibility leads to decreased device performance, delamination, inflammation, and discomfort. There is an unmet engineering and clinical need for epidermal bioelectronics that are bioinert, can emulate host tissue mechanical properties, demonstrate low bulk resistivity, and are flexible and scalable. To address this shortcoming, this work describes innovations pertaining to the development of a hydrophilic, biocompatible nanocomposite comprised of carbon black (CB), polyvinyl alcohol (PVA), and glycerol for neuro-muscular and rehabilitative applications. We find that this materials platform (herein referred to as CB-<i>AFTI</i>Derm), comprised of 3 wt% PVA and 5 wt% glycerol, demonstrated superior biocompatibility (cytotoxic grade of 0), high flexibility (maximum of 140% stretchability and as low as 1% ∆R/R_o at 3.5-cm bending diameter), low electrical resistivity (as low as 0.6 Ω.cm), and electrical stability over a long-term duration (at 235 Ω in the lateral direction and between 300 Ω and 400 Ω in the transverse direction for a 24 h period). We find that the optimal CB concentration for our material platform is at 50% CB. We present examples for use in electroceutical therapy of chronic wounds and in orthopedic rehabilitation for monitoring joint angles. Achieving such results from a material, mechanics, biological, and electrical perspective facilitates the translational potential of this materials platform for the digital health and wearable technologies community to improve patient outcomes.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35559","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Applied Pressure and Thickness Variation on the Bond Strength Between 3Y-TZP Zirconia and Self-Adhesive Resin Cement","authors":"Paulo Pinto,&nbsp;Óscar Carvalho,&nbsp;Rita Ferreira,&nbsp;Sara Madeira,&nbsp;Filipe S. Silva","doi":"10.1002/jbm.b.35563","DOIUrl":"https://doi.org/10.1002/jbm.b.35563","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluated the influence of applied pressure on the shear bond strength (SBS) between commercial 3Y-TZP zirconia and self-adhesive resin cement. Five test groups were created, each subjected to different applied pressures: 195.15, 97.47, 48.74, 9.75, and 0.20 kPa. A piston-type shear device was used to assess the effect of these pressures on cement layer thickness and SBS. The results demonstrated that the highest applied pressure (195.15 kPa) produced the thinnest cement layers (36.60 ± 1.67 μm) and the highest SBS values (58.8 ± 1.6 MPa), whereas the lower applied pressure (0.20 kPa) resulted in the thickest cement layers (73.20 ± 1.64 μm) and significantly lower SBS (30.8 ± 2.0 MPa). Regression analysis confirmed a strong correlation (<i>R</i>² = 0.82, <i>p</i> &lt; 0.05) between cement thickness and SBS, indicating that cement thickness is a critical factor influencing adhesion. These findings highlight the importance of controlling applied pressure during cementation to optimize bonding performance. Standardizing pressure application through calibrated pressure devices may improve the durability of zirconia-based restorations and minimize variability during the cementation process.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Perspectives on Additive Manufacturing and Titanium Surface Nanotopography in Bone Formation","authors":"Mariana Lima da Costa Valente,&nbsp;Lívia Maiumi Uehara,&nbsp;Rodolfo Lisboa Batalha,&nbsp;Claudemiro Bolfarini,&nbsp;Rayana Longo Bighetti Trevisan,&nbsp;Roger Rodrigo Fernandes,&nbsp;Marcio Mateus Beloti,&nbsp;Andréa Cândido dos Reis","doi":"10.1002/jbm.b.35554","DOIUrl":"https://doi.org/10.1002/jbm.b.35554","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to assess the impact of manufacturing methods (conventional and additive manufacturing) and surface treatments (polished and nanotopographic) on the physicochemical properties of Ti6Al4V alloy and their correlation with osteoblast cellular behavior. The evaluated groups were Machined Discs (MD), Machined Discs with Treatment (MD-WT), Additive-manufactured Discs (AD), and Additive-manufactured Discs with Treatment (AD-WT). Surface analyses included SEM, AFM, surface roughness, EDS, XRD, surface free energy, and zeta potential. MC3T3-E1 cells were cultured for biological assessments, including cell morphology, viability, gene expression, alkaline phosphatase activity, and mineralization. ANOVA and Holm-Sidak tests were applied (<i>p</i> &lt; 0.05). MD exhibited grooved topography, AD had partially fused spherical particles, while MD-WT and AD-WT showed patterns from chemical treatment (H₃PO₄ + NaOH). EDS identified additional ions in MD-WT and AD-WT. XRD patterns indicated crystal lattice orientation differences. MD-WT and AD-WT displayed higher surface free energy than MD and AD (<i>p</i> &lt; 0.05). AD had greater roughness (Sa 6.98 μm, <i>p</i> &lt; 0.05). Biological analyses revealed higher cell viability for MD and AD (<i>p</i> &lt; 0.001), higher ALP activity in MD, and lower in AD-WT. Gene expression varied, with MD showing higher <i>Alpl</i>, <i>Ibsp</i>, and <i>Bglap</i> (<i>p</i> &lt; 0.001), and AD-WT showing higher <i>Runx2</i> (<i>p</i> &lt; 0.001). Mineralized matrix behavior was similar for MD, AD, and MD-WT (<i>p</i> &gt; 0.05). MD and AD surfaces demonstrated superior osteogenic differentiation potential, while AD exhibited greater roughness, lower surface free energy, higher cell viability, and osteoblastic differentiation potential.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Mechanical Strength, Translucency, and Microstructure in Graded Zirconia Layers Before and After Hydrothermal Aging","authors":"Pablo Machado Soares,&nbsp;Luiza Freitas Brum Souza,&nbsp;Lucas Saldanha da Rosa,&nbsp;Luiz Felipe Valandro,&nbsp;Lucio Strazzabosco Dorneles,&nbsp;Alice Penteado Holkem,&nbsp;Paola de Azevedo Mello,&nbsp;Edson Irineu Müller,&nbsp;Atais Bacchi,&nbsp;Gabriel Kalil Rocha Pereira","doi":"10.1002/jbm.b.35550","DOIUrl":"https://doi.org/10.1002/jbm.b.35550","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to evaluate the monotonic and fatigue strength, translucency, topography, and grain size of different layers in two types of graded zirconia, both before and after hydrothermal aging. A total of 180 bar-shaped specimens (<i>n</i> = 15 per group) were prepared from each layer (cervical, transitional, and incisal) of two graded zirconias (IPS e.max ZirCAD MT Multi—4 mol% yttrium stabilized at the cervical region and 5 mol% at the incisal region; IPS e.max ZirCAD Prime, Ivoclar AG—3 mol% yttrium stabilized at the cervical region and 5 mol% at the incisal region) with dimensions of 14.0 × 4.0 × 3.0 mm, following ISO 6872 standards for a three-point-bending test. These specimens were divided into two groups: baseline and hydrothermal aging (134°C under 2 bars of pressure for 20 h). Monotonic and fatigue three-point bending tests (initial stress: 250 MPa/5000 cycles at 20 Hz; increments: 50 MPa/step) were performed until fracture. The translucency parameter (TP00) was calculated using the CIEDE2000 formula, and <i>L</i>*, <i>a</i>*, <i>b</i>* color coordinates were measured on disc-shaped specimens (<i>n</i> = 6 per layer; Ø = 10 mm, 1 mm thickness) before and after aging. Additional analyses included topography, fractography, and grain size. Data were analyzed using three-way ANOVA, Kaplan–Meier, and Mantel-Cox post hoc tests (log-rank). Hydrothermal aging decreased fatigue flexural strength across all layers and types of graded zirconia. After aging, the fatigue flexural strength of Prime zirconia was higher than that of MT Multi in the cervical layer. The cervical layers exhibited the highest flexural strength and smallest grain size, while incisal layers showed higher translucency compared to other layers. The induced aging protocol impacted the mechanical properties of the graded zirconia layers. Each layer within the multilayer zirconia systems displayed distinct mechanical, microstructural, and optical properties, with variations depending on the specific zirconia material. Overall, all layers demonstrated satisfactory performance, supporting the use of multilayer zirconia systems.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143535902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Compromised Maxillofacial Wound Healing Model for Characterization of Particulate Bone Grafting: An In Vivo Study in Rabbits","authors":"Nourhan Hussein,&nbsp;Vasudev Vivekanand Nayak,&nbsp;Neeraja Dharmaraj,&nbsp;Nicholas A. Mirsky,&nbsp;William Norton,&nbsp;Lori Ramagli,&nbsp;Ramesh Tailor,&nbsp;F. Kurtis Kasper,&nbsp;Paulo G. Coelho,&nbsp;Lukasz Witek,&nbsp;Simon Young","doi":"10.1002/jbm.b.35556","DOIUrl":"https://doi.org/10.1002/jbm.b.35556","url":null,"abstract":"<div>\u0000 \u0000 <p>Preclinical testing of tissue engineering modalities are commonly performed in a healthy wound bed. These conditions do not represent clinically relevant compromised oral wound environments due to radiation treatments seen clinically. This study aimed to characterize the bone regeneration outcomes in critical-sized mandibular defects using particulate grafting in an irradiated preclinical model of compromised wound healing. Sixteen New Zealand white rabbits were divided into two groups (<i>n</i> = 8/group), namely (i) irradiated (experimental) and (ii) non-irradiated (control). The rabbits in the experimental group received a total of 36 Gy radiation, followed by surgical intervention to create critical-sized (10 mm), full-thickness mandibular defects. The control group was subjected to the same surgical intervention. All defects were filled with bovine bone grafting material (Bio-Oss, Geistlich, Princeton, NJ, USA) and allowed to heal for 8 weeks. At the study endpoint, rabbits were euthanized, and their mandibles were harvested for micro-computed tomographic, histological, and histomorphometric processing and analysis. Qualitative histological analysis revealed increased levels of bone formation and bridging in the control group relative to the experimental group. This was accompanied by increased levels of soft tissue presence in the experimental group. Volumetric reconstruction showed a significantly higher degree of bone in the control group (27.59% ± 2.71), relative to the experimental group (22.02% ± 2.71) (<i>p</i> = 0.001). The irradiated rabbit model exhibited decreased bone regeneration capacity relative to the healthy subjects, highlighting its suitability as a robust compromised wound healing environment for further preclinical testing involving growth factors or customized, high-fidelity 3D printed tissue engineering scaffolds.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}