Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Correction to “In Vitro Reliability and Stress Distribution of Wide Diameter Extra-Short Implants as Support for Single Crowns and Fixed Partial Dentures”","authors":"","doi":"10.1002/jbm.b.35587","DOIUrl":"https://doi.org/10.1002/jbm.b.35587","url":null,"abstract":"<p>V. F. Vargas-Moreno, M. C. O. Ribeiro, R. S. Gomes, et al., “In Vitro Reliability and Stress Distribution of Wide Diameter Extra-Short Implants as Support for Single Crowns and Fixed Partial Dentures,” <i>Journal of Biomedical Materials Research</i> 113, no. 3 (2025): e35560. https://doi.org/10.1002/jbm.b.35560.</p><p>Figures 1 and 2 in this article are incorrect. They were plotted based on incorrect data from the pilot study analysis and not the correct final data from this study, which are described in the text of the article. The figures were swapped when the files were uploaded. The correct figures are below:</p><p>We apologize for this error.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35587","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diffusion of Hydrogen Peroxide Through Medical Grade Poly(Ether)urethane: Analyzing Mechanisms of Sorption and Transport to Support Sterilization With Vapor Phase Hydrogen Peroxide","authors":"Ioan P. I. Gitsov,&nbsp;Yunzhi Liu,&nbsp;David M. Saylor,&nbsp;Angela L. Hendrickx,&nbsp;Ann M. Gronda,&nbsp;Maruti N. Sinha,&nbsp;Eric Long,&nbsp;Shan Liu,&nbsp;Ruibo Hu,&nbsp;Jon W. Weeks,&nbsp;Michael Eppihimer,&nbsp;Kimberly A. Chaffin","doi":"10.1002/jbm.b.35567","DOIUrl":"https://doi.org/10.1002/jbm.b.35567","url":null,"abstract":"<p>The United States Food and Drug Administration (FDA) recently announced an update to their 510(k) medical device sterility guidance to include vapor phase hydrogen peroxide (VH2O2) as an established ‘Category A’ sterilization process. This places VH2O2 in the same category as ethylene oxide (EO or EtO), which has demonstrated user and patient safety as well as microbiocidal effectiveness through scientific literature and FDA-recognized consensus standards. For some implantable medical devices, the sterilant chemistry must diffuse through the polymers of construction to access sealed parts of the finished assembly to achieve an appropriate sterility assurance level. Diffusion of EO through materials has been well established over decades of successful use. However, the ability of VH2O2 to diffuse through materials of construction has not been demonstrated. In this work, we measured the diffusivity and permeability of VH2O2 for a series of increasing durometer poly(ether)urethanes (PEUs) commonly used in the construction of single-use medical devices. The diffusion coefficients were 1 × 10⁻⁸ cm²/s for PEU75D, 2 × 10⁻⁸ cm²/s for PEU55D, and 5 × 10⁻⁸ cm²/s for PEU80A. The permeabilities were calculated to be 4.7 × 10⁻⁶ cm²/s and 1.3 × 10⁻⁵ cm²/s for PEU55D and PEU80A, respectively. For a typical cardiac or neuromodulation lead, the PEU80A wall thickness is on the order of 0.013 cm, resulting in penetration of hydrogen peroxide into the sealed construction in less than 10 min, a timeframe that is a fraction of the total sterilization cycle time.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35567","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Impact of Polysaccharide-Based Nanoemulsions in Drug Delivery","authors":"Biswajit Basu,&nbsp;Srabona Dutta,&nbsp;Monosiz Rahaman,&nbsp;Swarnali Dutta,&nbsp;Mohd Nazam Ansari,&nbsp;Bhupendra G. Prajapati,&nbsp;Ayon Dutta,&nbsp;Sourav Ghosh","doi":"10.1002/jbm.b.35582","DOIUrl":"https://doi.org/10.1002/jbm.b.35582","url":null,"abstract":"<div>\u0000 \u0000 <p>Nanoemulsions are tiny mixtures of water and oil stabilized by surfactants, and they have become increasingly popular across various industries, including medicine. With droplet sizes in the nanometer scale, these mixtures are both compact and effective. This discussion explores the potential of polysaccharide-based nanotechnology as an innovative approach to drug delivery. Nanoemulsions offer several benefits, such as enhanced drug solubility and bioavailability, which are crucial for drugs that poorly dissolve in water. The incorporation of natural polysaccharides as emulsifiers in these nanoemulsions ensures their biocompatibility and safety within the body. Additionally, nanoemulsions can facilitate a sustained release of medications, allowing for gradual drug release over an extended period. This controlled release can be achieved through the careful selection and formulation of polysaccharides. This review addresses the methods for producing polysaccharide-based nanoemulsions and examines their physical and chemical properties. It highlights the influence of polysaccharide molecular weight and structure on the stability of nanoemulsions and the effectiveness of drug encapsulation. By understanding these factors, researchers can develop more efficient and safe drug delivery systems utilizing nanoemulsions. Additionally, the present article provides explicit and thorough information about the use of NPLS-based nano-carriers encapsulating a number of drugs designed to treat a variety of conditions, such as diabetes, cancer, HIV, malaria, cardiovascular and respiratory diseases, and skin diseases. For this reason, it is very important to review the most recent developments in polysaccharide-based nano-biocarriers in drug delivery and their application in the treatment of diseases. In this work, we concentrated on the preparation of polysaccharide-based nano-biocarriers, commonly used polysaccharides for the preparation of nano-biocarriers, and drugs loaded on polysaccharide-based nano-biocarriers to treat diseases. In the near future, polysaccharide-based nano-biocarriers will be used more and more frequently in drug delivery and disease treatment.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Local Sustained-Release of Triamcinolone-Acetonide-Loaded Regenerated Silk Fibroin Formulations for the Inhibition of Scar Hyperplasia in Rabbit Ears","authors":"Xinling Zhang,&nbsp;Xiaoxue Wang,&nbsp;Zhongyang Sun,&nbsp;Rongxin Ren,&nbsp;Jinping Ding,&nbsp;Wenjiang Qian,&nbsp;Hongyi Zhao,&nbsp;Jianjun Zhang,&nbsp;Shiwei Bao","doi":"10.1002/jbm.b.35578","DOIUrl":"https://doi.org/10.1002/jbm.b.35578","url":null,"abstract":"<div>\u0000 \u0000 <p>To investigate the safety, efficacy, and underlying mechanisms of triamcinolone-acetonide-loaded silk fibroin formulations in inhibiting scar hyperplasia in rabbit ears. This study employed molecular induction self-assembly and high-energy ball milling to prepare triamcinolone acetonide (Tr-A)-loaded sustained-release microspheres, RSF-Tr-A, using different inducers and concentrations of regenerated silk fibroin (RSF). Bio-safety was confirmed via CCK-8 and Live-Dead assays. The levels of growth factors and inflammatory cytokines were examined through RT-PCR. In a rabbit ear scar model, ultrasound Doppler assessed scar thickness and blood flow, a colorimeter recorded scar color changes, and Masson's trichrome staining evaluated collagen content and new collagen changes. CD31 immunohistochemistry determined vascular content in scarred skin. The RSF-Tr-A microsphere formulation was successfully prepared. In vitro tests showed good biosafety and significant inhibition of fibroblast proliferation and migration (<i>p</i> &lt; 0.001). It also promoted apoptosis (<i>p</i> &lt; 0.001) and reduced tube formation (<i>p</i> &lt; 0.01 and <i>p</i> &lt; 0.05). RT-PCR confirmed suppression of VEGF, EGF, bFGF, TGF-β1, IL-6, IL-1β, and TNF-α (<i>p</i> &lt; 0.001). In a rabbit ear scar model, VSS and SEI scores were significantly lower (<i>p</i> &lt; 0.05, <i>p</i> &lt; 0.01, <i>p</i> &lt; 0.001) and scar color difference was significantly different (<i>p</i> &lt; 0.001) at 5 W post-treatment. Histological analyses showed milder inflammation and collagen hyperplasia inhibition (<i>p</i> &lt; 0.05 and <i>p</i> &lt; 0.01) and reduced new blood vessel formation. The RSF-Tr-A microsphere formulation demonstrates good bio-safety and can effectively suppress fibroblast proliferation, collagen synthesis, inflammatory responses, and neovascularization through sustained release, thereby inhibiting scar hypertrophy.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The In Vivo Biological Response to Intra-Articular Injected Polycarbonate Urethane Wear Debris Particles","authors":"Jane A. E. Gruisen,&nbsp;Ilona M. Punt,&nbsp;Ryan Siskey,&nbsp;Pieter Emans,&nbsp;Aylvin Dias,&nbsp;Martijn Poeze,&nbsp;Alex K. Roth","doi":"10.1002/jbm.b.35579","DOIUrl":"https://doi.org/10.1002/jbm.b.35579","url":null,"abstract":"<p>Wear particles invariably form due to contact and friction between articulating surfaces in orthopedic prosthetic joint replacements. Polycarbonate urethane (PCU) has shown low wear rates and invoked minimal local biological response to wear debris in various orthopedic applications. However, controlled preclinical studies have not yet studied the biological response to PCU particles in synovial joints. This study aims to evaluate the biological response to mostly submicron-sized PCU wear particles in synovial joints in a rabbit model representing a worst-case scenario. PCU and ultra-high-molecular-weight-polyethene (UHMWPE) particles were generated in vitro, and particle characterization was performed using scanning electron microscopy (SEM) images. Fifteen New Zealand white rabbits, divided into three groups, received bilateral injections in the knee joint with 10 mg/mL PCU, UHMWPE particles, or saline (all 0.2 mL). After 3 months, the biological response in the joint was evaluated by histopathological reactivity scoring. The generated PCU and UHMWPE wear particles were mainly in the biologically active size range with an average equivalent circle diameter (ECD) of 0.31 μm (±0.48) and 6.99 μm (±16.32), respectively. There was a minimal to non-existing biological response (score ≤ 0.5) to PCU (0.5 ± 1.0), UHMWPE particles (0.6 ± 1.3) and saline (0.0 ± 0.0). Also, the wear particles did not disperse from the injection site. The results of this study support the use of PCU as a bearing surface in orthopedic prosthetic joint replacements by indicating that even in the likelihood that wear particles are generated, they are not likely to trigger a strong inflammatory response.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35579","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin-Conjugated Gold Nanoparticles Modulate Oxidative Stress and Antioxidant Activity and Maintain Intestinal Histoarchitecture in Drosophila melanogaster Larvae","authors":"Allisson Benatti Justino,&nbsp;Serena Mares Malta,&nbsp;Vinicius Prado Bittar,&nbsp;Maria Sol Peña Carrillo,&nbsp;Fernanda Naves Araújo do Prado Mascarenhas,&nbsp;Rafael Bernardes Ferreira,&nbsp;Alexandre Souza Marquez,&nbsp;Renata Graciele Zanon,&nbsp;Carlos Ueira-Vieira,&nbsp;Foued Salmen Espindola","doi":"10.1002/jbm.b.35581","DOIUrl":"https://doi.org/10.1002/jbm.b.35581","url":null,"abstract":"<p>Embryonic development is highly sensitive to oxidative stress, which can disrupt homeostasis. A strategy for mitigating oxidative stress induced by gold nanoparticles (AuNPs) involves the development of nanoparticles functionalized with phytoantioxidants through green chemistry methods, which also enhances the bioavailability of these antioxidants. In this study, environmentally friendly AuNPs were synthesized using curcumin (AuNPs-C), characterized by a spherical shape, uniform size, and a diameter of 7.2 ± 1.2 nm. The effects of AuNPs-C on oxidative stress in <i>Drosophila melanogaster</i> (Canton-S strain) during embryonic development were investigated, focusing on antioxidant defenses, oxidative damage, and morphological changes in the gastrointestinal tract. Exposure of <i>Drosophila</i> eggs to 50–200 μg/mL of AuNPs-C had no effect on hatching rates or pupal/adult development, with eclosion rates remaining above 50%. AuNPs-C did not elevate reactive oxygen species levels or induce lipid and protein oxidation in larvae exposed to 200 μg/mL. Oxidized protein products and malondialdehyde (MDA) levels remained comparable to those of the control group (70 ± 3 μM chloramine-T eq and 0.8 ± 0.1 nM MDA eq, respectively). Although AuNPs-C did not affect catalase activity or glutathione content, it reduced superoxide dismutase activity by 67% ± 6%. Additionally, AuNPs-C did not cause any damage to the gastrointestinal tract or alter the gut permeability of third-instar larvae. This study offers a deeper understanding of how AuNPs-C influence oxidative stress and antioxidant defense mechanisms in animal development and provides a basis for assessing the safety of phytoantioxidant-functionalized nanoparticles in vivo.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35581","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium Sodium Phosphosilicate in Management of Dentin Hypersensitivity and Strategies for Reconstitution of the Dentin Matrix","authors":"Ramya Ramadoss,&nbsp;Rajashree Padmanabhan","doi":"10.1002/jbm.b.35576","DOIUrl":"https://doi.org/10.1002/jbm.b.35576","url":null,"abstract":"<div>\u0000 \u0000 <p>Dentin hypersensitivity (DH) prevalence ranges from 72.5%–98% and is common from the third decade of life. Despite the variety of products available, dentine hypersensitivity remains a persistent issue. From the vast array of existing products, ceramics specifically Calcium sodium phosphosilicate (CSPS) based glasses seem to be the most versatile material to manage dentin hypersensitivity. Literature clearly indicates that Calcium sodium phosphosilicate (CSPS) permits a multitude of possibilities as it allows integration with soft and hard tissues. The ability to generate a flexible structure has resulted in an array of modifications. These characteristics are most vital and target specific to dentin than other biomaterials. Developing a long-lasting cure for dentin caries and dentin hypersensitivity will significantly improve the quality of life for patients. Even though it has been used for dentin remineralization for over two decades, CSPS has been able to achieve only partial success in terms of strength and crystalline pattern. CSPS can be put into more effective use in terms of dentin remineralization by in-depth understanding of the dynamic matrisome and the mineralization process. CSPS can be optimized as a smart delivery method and applied as a sealant for pits and fissures in a comprehensive preventive dental caries initiative. CSPS-based drug delivery systems can effectively reconstitute the key molecules of the dentin matrix that are commonly damaged during dentinal caries or other pathologies. Additional adjustments to the mix can facilitate the development of dentin-bonding agents. This review seeks to summarize the studies on CSPS on dentin hypersensitivity, and it also elaborates on possible futuristic approaches to reconstitute the dentin matrix for effective management of DH.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Glasses as Alternatives to Hydroxyapatite for Bone Implant Coatings: A Comparative Study","authors":"B. Garrido,&nbsp;N. Garcia-Giralt,&nbsp;M. A. Rodríguez,&nbsp;A. H. de Aza,&nbsp;I. G. Cano","doi":"10.1002/jbm.b.35577","DOIUrl":"https://doi.org/10.1002/jbm.b.35577","url":null,"abstract":"<div>\u0000 \u0000 <p>No material currently matches the remodeling rates of natural bone. Although hydroxyapatite is commonly employed as a bioactive coating material for implants, its resorption rate is relatively low. Conversely, bioactive glass materials offer versatility, as they can be tailored with various oxides and proportions to alter their structure and, consequently, their properties. This study examines various bioactive glasses as potential alternatives to hydroxyapatite coatings used in bone implants. Coatings were fabricated using commercial compositions, 45S5 and S53P4, and the 62W composition, which is not yet commercially available. Atmospheric Plasma Spray was utilized to deposit the different bioactive materials onto titanium alloy substrates. The coatings obtained underwent analysis via scanning electron microscopy and x-ray diffraction. It was observed that all bioactive glass coatings exhibited an amorphous structure, with the 62W coating showing less porosity. Mechanical and in vitro tests were conducted to assess their properties. The composition of the bioactive glasses significantly influenced the bond strength and bioactive capacity of the coatings, as well as the cellular response of the coating. The study suggests that bioactive glasses, especially the 62W composition, represent a considerable alternative as coating material.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical Modeling and Validation of Radial Stiffness in Zigzag-Shaped Stents: Experimental and Numerical Approaches","authors":"Moshe Brand,&nbsp;Nadav Sharon","doi":"10.1002/jbm.b.35574","DOIUrl":"https://doi.org/10.1002/jbm.b.35574","url":null,"abstract":"<p>Stents and stent grafts (SGs) are used in many endovascular procedures. Clinical studies have revealed that radial stiffness (RS) in stents increases pressure on the artery wall, which can cause restenosis and, in some cases, require repeated intervention. This study aimed to validate an analytical model for calculating the RS of stents and SG with a zigzag-shaped structure. Four commercial SGs with varying geometric dimensions and material properties were selected. The analytical model's RS calculations were compared with experimental measurements and numerical model predictions. An excellent agreement was observed between the RS values measured in the experimental system and those calculated in the analytical model, with an average difference of 2.4% ± 3.3%. Validation of the analytical model enables the design of stents with a desired RS that is optimally suited to a specific patient.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35574","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immobilization of Antibacterial Chlorhexidine on Biodegradable Polycaprolactone/Estradiol Electrospun Nanofibrous Membrane for Bone Regeneration","authors":"Dida Faadihilah Khrisna,&nbsp;Jaweria Ambreen,&nbsp;Tan Yu Wei,&nbsp;Nurul Aina Haziqah Riduan,&nbsp;Nurizzati Mohd Daud,&nbsp;Norhidayu Muhamad Zain,&nbsp;Faizuan Abdullah,&nbsp;Nik Ahmad Nizam Nik Malek,&nbsp;Mokhamad Fakhrul Ulum,&nbsp;Syafiqah Saidin","doi":"10.1002/jbm.b.35575","DOIUrl":"https://doi.org/10.1002/jbm.b.35575","url":null,"abstract":"<div>\u0000 \u0000 <p>Membrane-based scaffold for bone regeneration is vastly being explored to address issues that persist in defective bone regeneration, associated with infection and inflammation. This study focused on incorporating estradiol (E2) into biodegradable polycaprolactone (PCL) electrospun nanofibrous membrane, followed by the immobilization with antibacterial chlorhexidine (CHX) through the aid of a polydopamine (PDA) grafting layer. Several analyses including field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), wettability, biodegradation, drug release, antibacterial, and cytotoxicity analyses were conducted to study the physicochemical and biological properties of the membranes. The nanofibers were constructed with an average diameter of 1.32–1.33 μm and a porosity of 51%–53%, which is accommodating bone regeneration. The grafting of PDA was not only able to improve the surface wettability, which in turn allowed controllable degradability and sustained the release of E2 and CHX from the nanofibrous membranes. The immobilization of CHX on the PCL/E2 nanofibers has greatly retarded Gram-negative <i>Escherichia coli</i> compared to Gram-positive <i>Staphylococcus aureus</i>. The in vitro cytotoxicity assay statistically depicted the ability of the fabricated nanofibrous membranes to support cell proliferation without cytotoxic effects at the cell viability above 70%. These cumulative results indicate the potential development of CHX-immobilized PCL/E2 membrane as an alternative strategy to resolve bone regeneration issues.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}