Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Calcium Phosphate Apatite Filament Co-Wrapped With Perforated Electrospun Sheet of Phosphorylated Chitosan—A Bioinspired Approach Toward Bone Graft Substitute","authors":"Prabhash Dadhich,&nbsp;Pallabi Pal,&nbsp;Nantu Dogra,&nbsp;Pavan K. Srivas,&nbsp;Bodhisatwa Das,&nbsp;Samir Das,&nbsp;Pallab Datta,&nbsp;Baisakhee Saha,&nbsp;Bo Su,&nbsp;Santanu Dhara","doi":"10.1002/jbm.b.35589","DOIUrl":"https://doi.org/10.1002/jbm.b.35589","url":null,"abstract":"<div>\u0000 \u0000 <p>Bioinspired bone graft substitutes hold incredible opportunities in tissue engineering, potentiating the healing aspect. Here we have fabricated stacks of glutaraldehyde–genipin crosslinked, microporous nanofibrous <i>N</i>-methyl phosphonic chitosan sheets (NMPC) with impregnated eggshell-derived CaP fibers to mimic osteonal architecture. This composite 3D rolled eggshell-derived calcium phosphate (ESCAP) scaffold (RCS), with density and modulus variation from the center to the periphery, has superior mechanical strength. The zwitterionic nature of NMPC, following the surface modulus of the CaP fibers, upgraded the biological performance. The low modulus of the flexible micro-perforated nanofibrous sheet increases along the ceramic phase, which prompts migration and distribution of proliferated MSCs from the outer polymeric surface to the inner ceramic region through micro-perforations. This movement stimulates endochondral ossification, observed by a gradual increment of collagen II expression alongside a decrement of collagen I expression. In vivo assessment of rabbit tibia bone defects revealed prominent healing in the presence of a scaffold by Day 60, accompanied by scaffold resorption. The cellular activity during healing revealed osteoblasts, osteocytes, blood vessels, and chondroblast cells at the boundary of the scaffolds, indicating neotissue and hypertrophic cartilage formation. Thus, the RCS bone grafts promote faster bone healing by osteogenesis and bone remodeling.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carrageenan-Based Hydrogels for Advanced Wound Healing and Controlled Drug Delivery in Tissue Engineering","authors":"Great Iruoghene Edo,&nbsp;Winifred Ndudi,&nbsp;Raghda S. Makia,&nbsp;Irene Ebosereme Ainyanbhor,&nbsp;Emad Yousif,&nbsp;Tayser Sumer Gaaz,&nbsp;Endurance Fegor Isoje,&nbsp;Rapheal Ajiri Opiti,&nbsp;Patrick Othuke Akpoghelie,&nbsp;Ufuoma Augustina Igbuku,&nbsp;Dina S. Ahmed,&nbsp;Arthur Efeoghene Athan Essaghah,&nbsp;Huzaifa Umar","doi":"10.1002/jbm.b.35594","DOIUrl":"https://doi.org/10.1002/jbm.b.35594","url":null,"abstract":"<div>\u0000 \u0000 <p>Carrageenan (CGN) is a high molecular weight polysaccharide that is extracted from red seaweeds. It is made up of D-galactose residues connected by β-1,4 and α-1,3 galactose-galactose bonds. As a result of its ability to thicken, emulsify, and stabilize food, it is frequently used as a food additive in processed food. Its consumption has surged in recent years due to the Western diet's (WD) spread. Carrageenan has the ability to change the thickness of the mucus barrier, the composition of the gut microbiota, and the innate immune pathway that causes inflammation. Also, its inherent qualities, which include biodegradability, biocompatibility, resemblance to native glycosaminoglycans, antioxidants, anticancer, immunomodulatory, and anticoagulant activities, Carrageenan-based hydrogels have been the subject of numerous investigations lately for biomedical applications. The brittle hydrogel and uncontrollably exchanged ions, however, are two drawbacks to the application of this polysaccharide, but these can be avoided by making straightforward chemical changes to polymer networks, which create chemically bonded hydrogels with important mechanical characteristics and regulated degradation rates. Furthermore, the addition of diverse kinds of nanoparticles, as well as polymer networks, to carrageenan hydrogels results in hybrid platforms with noteworthy mechanical, chemical, and biological characteristics, which qualify them as appropriate biomaterials for tissue engineering (TE), drug delivery (DD), and also wound healing applications. Our goal in this article is to provide an overview of the most current developments in hybrid carrageenan-based platforms and several chemical modification techniques for TE and DD applications.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Degradation and Cytocompatibility Study of Biodegradable Porous Zinc Scaffolds Fabricated by Fused Deposition Modeling Based Rapid Tooling Method","authors":"Abhishek Kansal,&nbsp;Sangita Mahapatra,&nbsp;Akshay Dvivedi,&nbsp;Pradeep Kumar,&nbsp;Mayank Goswami","doi":"10.1002/jbm.b.35593","DOIUrl":"https://doi.org/10.1002/jbm.b.35593","url":null,"abstract":"<div>\u0000 \u0000 <p>An integrative study is conducted on the static biodegradation behavior and cytocompatibility of organized porous network structured (OPNS) zinc scaffolds fabricated via the Fused Deposition Modeling based Rapid Tooling (FDM-RT) method. The degradation study investigates the corrosion mechanism over immersion time in simulated body fluid (SBF), whereas the cytocompatibility study incorporates MTT assay and direct cell counting tests. The results indicate that the scaffold morphology, including scaffold struts, interconnectivity, or porosity, along with the immersion period, significantly influence the degradation behavior. The static corrosion rates are determined to be 0.27 ± 0.006, 0.79 ± 0.01, and 1.35 ± 0.05 mm y⁻¹ for bulk and porous zinc samples after 28 days. The higher corrosion rate of porous zinc alloys is mainly due to their higher surface area, which enhances exposure to the solution. Over time, protective layers form on both porous and bulk samples, leading to a gradual decrease in corrosion rate. The XRD results reveal that the corrosion protective layer is primarily composed of ZnO, Ca₃(PO₄)₂, Zn (OH)₂, and Zn₃(PO₄)₂·4H₂O. Moreover, MTT assay and direct cell counting of mouse fibroblast 3T3 cells on bulk and porous zinc samples suggest that the prepared zinc samples have potential for tissue engineering applications.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143939408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Collagen and Polylactic Acid Multifilament Yarns for Developing Textile Scaffolds for Tendon Tissue Engineering Applications","authors":"Yihan Huang,&nbsp;Robert Tonndorf,&nbsp;Jessica M. Gluck,&nbsp;Edwin R. Cadet,&nbsp;Martin W. King,&nbsp;Jacqueline H. Cole","doi":"10.1002/jbm.b.35583","DOIUrl":"https://doi.org/10.1002/jbm.b.35583","url":null,"abstract":"<p>This study is the first to compare the mechanical properties and biocompatibility of multifilament yarns made from a natural biopolymer, collagen, and from a synthetic polymer, polylactic acid (PLA), with a view toward evaluating the suitability of these two yarns for fabricating tendon tissue engineering scaffolds. Genipin, the natural plant-based anti-inflammatory and antioxidant crosslinking agent, was used to stabilize the collagen structure. A range of different genipin concentrations, crosslinking temperatures, and crosslinking times were studied, and based on higher degrees of crosslinking, reduced swelling ratio, and greater tensile strength, the recommended conditions are crosslinking the wet-spun collagen yarns with 1.0% genipin at 37°C for 72 h. During a 6-week enzymatic degradation study, the PLA yarns were more resistant to degradation and retained greater tensile strength than the genipin-crosslinked collagen yarns under both dry (4.0 ± 0.2 N vs. 2.2 ± 0.4 N) and hydrated (3.7 ± 0.3 N vs. 0.4 ± 0.1 N) conditions. After coating the PLA yarns with collagen, their biocompatibility improved, and they were able to support tendon cell growth and proliferation. In conclusion, the selection of multifilament PLA yarns coated with collagen is recommended as a promising scaffold material for tendon tissue engineering applications.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35583","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Versatile Biomaterial Additive: A Game-Changing Multifunctional Synthetic Peptide With Pro-Regenerative, Anti-Inflammatory, and Antibacterial Properties","authors":"Mirosława Panasiuk,&nbsp;Milena Chraniuk,&nbsp;Piotr Bollin,&nbsp;Justyna Sawicka,&nbsp;Anna Sylla,&nbsp;Lilit Hovhannisyan,&nbsp;Monika Biernat,&nbsp;Sylwia Rodziewicz-Motowidło,&nbsp;Beata Gromadzka","doi":"10.1002/jbm.b.35591","DOIUrl":"https://doi.org/10.1002/jbm.b.35591","url":null,"abstract":"<div>\u0000 \u0000 <p>The prevalence of bone disorders and deformities is increasing due to trauma, malignant neoplasms, infections, and degenerative and inflammatory diseases. Bone repair and replacement have evolved as a consequence of advancements in orthopedic technology and biomaterials with enhanced properties. The rapid growth of the bone tissue engineering field is being significantly influenced by biomaterials, such as polymer scaffolds with appropriate surface modifications. New additives are constantly being developed in response to the increasing demand for enhancing the bioactivity of biocomposites used for bone regeneration. We present the design and synthesis of a synthetic bioactive peptide UG46, which is multifunctional and consists of the fragment of human Cystatin C (CystC) and anoplin. In addition, the peptide is assessed as an additive that is employed to enhance the repair of bone by enriching porous chitosan (CH) scaffolds. Our results indicated that the UG46 peptide possesses pro-regenerative properties, while it did not exhibit any cytotoxic effects on human osteoblasts or human fibroblasts. Incubation of cells with CH and CH-UG46 extracts significantly increased cell proliferation, cell proliferation over 200% of control cells. Even though the proliferation assay revealed a significant inhibition of cell proliferation in cells seeded directly on the composites, the beneficial effect of the UG46 peptide was still noticeable. Additionally, the UG46 peptide exhibited dose-dependent anti-inflammatory properties in both its free form and as a biocomposite additive, qualifying it as a promising candidate for a bone biomaterial component. The working concentrations of UG46 peptide have been established at 40–80 μg/mL. The molecular structure analysis of the CH-UG46 biocomposites revealed that the majority of the pores were sufficiently large to enable osteoblast cells to infiltrate the scaffold, while concurrent microporosity (&lt; 20 μm) enabled cell infiltration, vascularization, and cell-matrix interactions. Additionally, the peptide alone exhibits limited antibacterial properties; however, the peptide released from the CH-UG46 biocomposite at high levels has been demonstrated to be capable of inhibiting the growth of the selected bacterial strains that are most frequently found infecting healing wounds by over 83% for all strains tested stains with A. baumani bacterial count reduction at 99.86%. Our findings indicate that the bioactive peptide we have proposed is a promising enhancement for porous scaffolds. It has the potential to facilitate the creation of specialized, custom-designed biomaterials with multifunctional properties, including healing, defense, and hygiene.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-Pot Synthesis of Antibacterial and Antioxidant Self-Healing Bioadhesives Using Ugi Four-Component Reactions","authors":"Ronak Afshari,&nbsp;Arpita Roy,&nbsp;Saumya Jain,&nbsp;Kaimana Lum,&nbsp;Joyce Huang,&nbsp;Sam Denton,&nbsp;Nasim Annabi","doi":"10.1002/jbm.b.35584","DOIUrl":"https://doi.org/10.1002/jbm.b.35584","url":null,"abstract":"<div>\u0000 \u0000 <p>Bioadhesive materials are extensively utilized as alternatives to surgical sutures and wound dressings. Despite significant advancements in their synthesis, current bioadhesives suffer from inadequate mechanical stability, suboptimal wet tissue adhesion, and a lack of inherent antibacterial and antioxidant properties, while requiring multistep synthesis processes, complicating their production for biomedical applications. To address these limitations, we developed a new bioadhesive, named UgiGel, synthesized through a one-pot Ugi four-component reaction (Ugi-4CR). Our strategy utilized gelatin as the backbone, 4-formylphenylboronic acid (4-FPBA) as an aldehyde source for improved adhesion and antibacterial activity, gallic acid (GA) as a carboxylic acid source for improved antioxidant activity and wound healing, and cyclohexyl isocyanide (CyIso) to induce pseudopeptide structures. The internal crosslinking between GA and 4-FPBA via dynamic boronate ester bond formation, triggered by slight pH changes (7.4–7.8) and temperature elevation (25°C–40°C), resulted in the formation of viscoelastic and self-healing hydrogels with water as the only byproduct without the need for initiator/light activation. UgiGel showed higher adhesion to porcine skin tissue (139.8 ± 8.7 kPa) as compared to commercially available bioadhesives, Evicel (26.3 ± 2.6 kPa) and Coseal (19.3 ± 9.9 kPa). It also demonstrated effective antibacterial properties against both Gram-negative and Gram-positive bacteria, as well as antioxidant activity. Additionally, the in vitro studies using NIH-3T3 cells confirmed the biocompatibility of the UgiGel over 7 days of culture. Moreover, in vivo biocompatibility and biodegradation of UgiGel were confirmed via subcutaneous implantation in rats for up to 28 days. Our results demonstrated that UgiGel outperformed commercially available bioadhesives in terms of adhesion, self-healing, and antibacterial activity, without compromising biocompatibility or physical properties, representing a promising multifunctional bioadhesive for wound sealing and repair.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143897117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Effects of Nano TiO2 and HA Reinforcement Ratio on Mechanical, Morphological, and Thermal Properties of PLA Matrix Bio Composites","authors":"Hatice Evlen,&nbsp;Sümeyye Ceren Eroğlu","doi":"10.1002/jbm.b.35590","DOIUrl":"https://doi.org/10.1002/jbm.b.35590","url":null,"abstract":"<p>Although bone tissue has the ability to regenerate itself, this regeneration capacity may be limited in large defects or pathological conditions. The development of biomaterials and tissue scaffolds is of critical importance in supporting bone regeneration. In this context, polymer nanocomposites, which are increasingly gaining interest in bone tissue engineering, benefit from both the flexibility of the polymer and the mechanical strength of inorganic components by dispersing nano-sized fillers in the polymer matrix. This study is important in terms of the multifaceted characterization of the hybrid composite material formed with optimized reinforcement ratios of polylactic acid (PLA), hydroxyapatite (HA), and titanium dioxide (TiO₂) components. The originality of the study stems from the comprehensive examination of the mechanical, morphological, thermal, and biological properties of the material in question and the determination of the optimum reinforcement range in light of data obtained from the studies of different researchers in the literature. This multiparameter and holistic approach contributes to the expansion of the potential application areas of the material and the development of a more in-depth understanding of the field of materials science. This study aims to investigate the thermal, mechanical, and morphological effects of HA and TiO₂ reinforcement and reinforcement ratio on nano PLA matrix material. To synthesize these composites, 10% nano HA and different ratios of nano TiO₂ (1%, 2%, and 3%) were added to the nano PLA matrix material. Specimens were prepared by using the casting particle removal method. For the biocompatibility test of the samples, all composite samples were immersed for 1–4 weeks in simulated body fluid (SBF). For the investigation of mechanical, morphological, and thermal properties, SEM, EDS, XRD, DTA, DCS, TG analyses, and compression tests were performed. As a result, it was observed that the apatite layer on the sample surfaces gradually thickened as the residence time in the SBF increased, and the HA and TiO₂ reinforcement to the matrix material supported the formation of the apatite layer. Also, the highest mass loss was seen in PLA/HA samples. The decomposition temperature of the composites decreased with the addition of HA and TiO₂ to the PLA matrix material. In addition, it has been observed that increasing the TiO₂ reinforcement ratio improves the mechanical properties of the composite and increases its strength.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35590","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Zn-Modified Hydroxyapatite Whiskers on Physicochemical and Biological Properties of Poly(ε-Caprolactone) Composites Intended for Implantable Medical Devices","authors":"Monika Biernat,&nbsp;Joanna Pagacz,&nbsp;Paweł Piszko,&nbsp;Małgorzata Siwińska,&nbsp;Emilia Zachanowicz,&nbsp;Sylwia Michlewska,&nbsp;Agnieszka Antosik,&nbsp;Paulina Tymowicz-Grzyb,&nbsp;Anna Sylla,&nbsp;Piotr Szterner,&nbsp;Adrian Najmrodzki,&nbsp;Mateusz Urbaniak,&nbsp;Paulina Rusek-Wala,&nbsp;Aleksandra Szwed-Georgiou,&nbsp;Karolina Rudnicka,&nbsp;Konrad Szustakiewicz","doi":"10.1002/jbm.b.35586","DOIUrl":"https://doi.org/10.1002/jbm.b.35586","url":null,"abstract":"<p>Poly(ε-caprolactone) (PCL)-hydroxyapatite (HAP) biocomposites were produced by thermal processing to test the impact of HAP addition on the physicochemical and biological properties of PCL. Two different HAPs: zinc-modified and unmodified, were added to the polymer matrix to enhance their biocompatibility, surface properties, and antimicrobial activity. The overall properties of biocomposites were assessed by thermal and mechanical analysis, while their structure and morphology were assessed by electron microscopy and infrared spectroscopy. A short-term degradation process of the composites in terms of their medical application was carried out, and biocompatibility was investigated regarding cytocompatibility, immunocompatibility, and bactericidal activity. PCL/HAP composites with 15 wt.% HAP offer the best-balanced properties with a moderate decrease in mechanical strength, cytocompatibility, and a moderate increase in antimicrobial activity. All the composites show high cytocompatibility with both L929 fibroblasts and hFOB 1.19 human fetal osteoblasts. Zn modification promoted their antimicrobial properties, and they have been proven safe for use in a short degradation test. Therefore, the PCL/HAP and PCL/HAP_Zn biocomposites have potential for medical applications, especially for bone regeneration.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35586","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Different Geometry Poly(L-Lactide-Co-Glycolide-Co-Trimethylene Carbonate Oligomer) Scaffolds Fabricated by Material Extrusion 3D Printing for Adipose Derived Stem Cells Culture","authors":"Piotr Paduszyński,&nbsp;Jakub Włodarczyk,&nbsp;Jakub Rok,&nbsp;Małgorzata Pastusiak,&nbsp;Zuzanna Rzepka,&nbsp;Agnieszka Ochab,&nbsp;Paulina Karpeta-Jarząbek,&nbsp;Arkadiusz Orchel,&nbsp;Dorota Wrześniok,&nbsp;Janusz Kasperczyk","doi":"10.1002/jbm.b.35580","DOIUrl":"https://doi.org/10.1002/jbm.b.35580","url":null,"abstract":"<div>\u0000 \u0000 <p>The combination of stem cells, growth factors, and biomaterials has driven significant advancements in tissue engineering. Depending on the specific tissue requiring regeneration, the scaffold structure and cell type must be carefully selected. Adipose-derived stem cells (ADSC) have garnered considerable interest due to their ease of isolation and high differentiation potential. However, cellular components alone are often insufficient for complete tissue regeneration, making the selection of an appropriate scaffold structure a critical factor. Modern additive manufacturing techniques enable the precise design and fabrication of scaffolds with tailored properties and architectures. This study presents comprehensive research in tissue engineering, polymer chemistry, and polymer processing, focusing on the fabrication of scaffolds with varying architectures for ADSC culture using additive manufacturing. A poly(L-lactide-co-glycolide-co-trimethylene carbonate oligomer) (PLGA-oTMC) terpolymer of defined molar composition and microstructure was synthesized and processed into a filament suitable for 3D printing via the Material Extrusion (formerly Fused Deposition Modeling) method, which has not yet been demonstrated in scientific research. Optimized molar composition, microstructure, and average molar mass of PLGA-oTMC ensured an appropriate melt viscosity, facilitating 3D printing under conditions that minimized polymer thermal degradation. This, in turn, enabled effective cell culture. The resulting scaffolds exhibited favorable biocompatibility, as evidenced by high ADSC viability and proliferation capacity. However, variations in scaffold architecture influenced ADSC colonization, with certain designs promoting more effective adhesion and cytoskeletal organization. The good viability and proliferative ability of ADSC strongly suggest that PLGA-oTMC scaffolds, combined with stem cells, show great promise for the engineering of damaged tissues such as bone or cartilage.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocellulose Technologies: Production, Functionalization, and Applications in Medicine and Pharmaceuticals - A Review","authors":"Mohammad Al-Zu'bi,&nbsp;Mizi Fan","doi":"10.1002/jbm.b.35585","DOIUrl":"https://doi.org/10.1002/jbm.b.35585","url":null,"abstract":"<div>\u0000 \u0000 <p>This review provides a comprehensive analysis of nanocellulose production, characterization, and applications, with a particular focus on its use in membranes and films for healthcare applications. The diverse sources of nanocellulose, including wood-based materials, agricultural byproducts, algae, and bacteria, are explored, highlighting their renewability, environmental benefits, and adaptability for specialized applications. The review also examines various pretreatment and processing methods, such as mechanical, chemical, and enzymatic treatments, outlining their roles in achieving desirable nanocellulose properties. Additionally, surface modification techniques, including amidation and esterification, are discussed for enhancing compatibility, stability, and performance when nanocellulose is integrated into composite materials. A novel mechanochemical approach is highlighted as a sustainable and energy-efficient fibrillation technique that reduces the environmental impact of nanocellulose production. Furthermore, the chemical modification and functionalization of nanocellulose are analyzed to expand its capabilities in advanced biomedical applications, including tissue engineering scaffolds that provide structural support for cell growth, wound dressings that leverage nanocellulose's antimicrobial and moisture-retentive properties, and drug delivery systems that utilize its biocompatibility and tunable release characteristics. The review concludes with future research directions, emphasizing the need for continued optimization of processing techniques, hybrid material development, and stimuli-responsive nanocellulose systems to unlock new biomedical and industrial applications.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}