Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Towards Mechanical Compatibility: Optimization of an Implant Used in Ventral Hernia Repair","authors":"Szymon Kalinowski,&nbsp;Katarzyna Szepietowska,&nbsp;Éric Florentin,&nbsp;Izabela Lubowiecka","doi":"10.1002/jbm.b.35650","DOIUrl":"10.1002/jbm.b.35650","url":null,"abstract":"<div>\u0000 \u0000 <p>Effective treatment of abdominal hernia with synthetic implants requires a prosthetic material biologically and mechanically compatible with the tissue. The mechanical compatibility is particularly important because the human abdominal wall is a complex multilayer structure and its properties may have individual characteristics that are not fully known. To address this issue, we propose a novel approach to optimal implant design for hernia repair by modifying locally the implant thickness to adapt it to the applied loads. Compatibility criteria are translated to an objective function that is to be minimized in the optimization procedure. The objective function is designed to equalize and minimize forces at the tissue-implant interface and minimize implant deflection. This reduces vulnerability to failure without hindering functionality. The input data are taken from in vivo tests on human subjects performed using digital image correlation and applied to a computational model of the implant defined by means of the Finite Element Method. The results show that the material distribution varies across models with different properties in two perpendicular directions (i.e., orthotropy) and across individuals, suggesting the potential for patient-specific design of the implant and a patient-specific approach to hernia repair. This approach takes into account abdominal wall heterogeneity and anisotropy, which in practice may help to reduce the ventral hernia recurrence rate.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical Evaluation of Boron Nanoparticle-Modified Silicone Elastomers for Maxillofacial Prostheses","authors":"Naim Berker Altuntaş,&nbsp;Canan Akay,&nbsp;Esra Nur Avukat","doi":"10.1002/jbm.b.35659","DOIUrl":"10.1002/jbm.b.35659","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluated the effects of boron nanoparticles (BNPs) on the mechanical properties of two silicone elastomers, A-2000 and A-2006. Tensile, tear, hardness, and elongation tests were conducted in accordance with ASTM and ISO standards. A total of 180 specimens were prepared, comprising control groups without BNPs and experimental groups containing 1 and 3 wt% BNPs. Tensile and tear strength tests were performed using a device with a 1 kN capacity at a crosshead speed of 100 mm/min; hardness was measured using Shore A tests, and Atomic Force Microscopy (AFM) was employed to assess surface roughness. Tensile testing revealed that the A-2000 control group exhibited the highest tensile strength, with significant reductions observed in both BNP-incorporated subgroups. In A-2006, tensile strength decreased significantly with 1 wt% BNPs but partially recovered at 3 wt%. Tear strength in A-2000 significantly decreased at 1 wt% but returned to control levels at 3 wt%, whereas no statistically significant differences were observed among the A-2006 subgroups. Hardness significantly increased with 3 wt% BNPs in A-2000 and with both 1 and 3 wt% BNPs in A-2006. Regarding elongation, A-2000 showed no significant change compared with the control, although the 1 and 3 wt% groups differed significantly from each other. In A-2006, both 1 and 3 wt% BNP groups showed significant reductions in elongation compared with the control. Overall, A-2000 exhibited superior tensile and tear strength, while A-2006 demonstrated greater elongation capacity. These findings indicate that BNP incorporation depends on both the elastomer type and concentration, with potential trade-offs between improved hardness and decreased flexibility. Both A-2000 and A-2006 remain viable options for maxillofacial prostheses, although optimization of BNP concentration is essential to balance strength, durability, and flexibility.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Bovine and Porcine Collagen Membranes for Potential Applications in Guided Bone Regeneration: An In Vivo Pre-Clinical Evaluation","authors":"Vasudev Vivekanand Nayak,&nbsp;Joao Arthur Kawase De Queiroz Goncalves,&nbsp;Nicholas A. Mirsky,&nbsp;Aris R. L. Arakelians,&nbsp;Edmara T. P. Bergamo,&nbsp;Andrea Torroni,&nbsp;Daniel Boczar,&nbsp;Paulo G. Coelho,&nbsp;Lukasz Witek","doi":"10.1002/jbm.b.35651","DOIUrl":"https://doi.org/10.1002/jbm.b.35651","url":null,"abstract":"<div>\u0000 \u0000 <p>In an effort to improve bone response, predictably regenerate lost tissue, and provide an anatomically pleasing ridge contour for biomechanically favorable and prosthetically driven implant placement, guided bone regeneration (GBR) procedures have been indicated. This study provides the first direct in vivo comparison of the biocompatibility of an experimental porcine-derived collagen membrane (CMI, Regenity Biosciences, Paramus, NJ, USA) and a commercially available bovine-derived collagen membrane (CopiOs, ZimVie, Palm Beach Gardens, FL, USA) in a <i>beagle</i> mandibular model for the purposes of GBR. Four bilateral defects of 10 mm × 10 mm through the mandibular thickness were placed in each of <i>n</i> = 16 adult <i>beagle</i> dogs. Defects were filled with a deproteinized porcine-derived particulate graft and were covered either with CMI or CopiOs to allow compartmentalized healing. Animals were euthanized after 4, 8, 12, or 16 weeks post-operatively (<i>n</i> = 4 <i>beagles</i>/time point). Bone regenerative capacity, graft, and soft tissue presence were evaluated by histomorphometric and microtomographic analyses. Outcome variables were compared using a mixed model analysis with fixed factor variables of time and material. Qualitatively, no histomorphological differences in healing were observed between the membrane groups at any time point. Histomorphometrically, CMI and CopiOs presented statistically significant differences in bone (mean ± SD: 38.27% ± 15.20 vs. 17.43% ± 15.49, respectively, <i>p</i> = 0.016) and soft tissue presence (mean ± SD: 50.88% ± 11.83 vs. 68.21% ± 16.98, respectively, <i>p</i> = 0.026) at 8 weeks. These results might influence treatment timing in clinical practice, by enabling early implant placement or shorter healing intervals. No significant differences were detected in these parameters at any other healing time point (<i>p</i> &gt; 0.05). CMI and CopiOs showed no signs of adverse immune response and led to similar trends in bone regeneration after 16 weeks of permitted healing. Both membranes minimized soft tissue infiltration and maintained defect stability over the observed healing periods without adverse events such as inflammation and/or foreign body reaction.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Resin Composite Filling Thickness in Zirconia Abutment Screw-Access on the Fatigue Behavior of a Cement-Retained Lithium Disilicate Material","authors":"Maria Gabriela Packaeser,&nbsp;Fernanda Cocco,&nbsp;Lucas Saldanha da Rosa,&nbsp;João Paulo Mendes Tribst,&nbsp;Cornelis Johannes Kleverlaan,&nbsp;Luiz Felipe Valandro,&nbsp;Gabriel Kalil Rocha Pereira,&nbsp;Pablo Machado Soares","doi":"10.1002/jbm.b.35657","DOIUrl":"10.1002/jbm.b.35657","url":null,"abstract":"<p>This study evaluates the effect of varying resin composite thicknesses for sealing the screw-access hole of zirconia abutments on the fatigue mechanical behavior of a lithium disilicate cement-retained material. One hundred lithium disilicate discs (<i>Ø</i> = 10 mm, 1 mm thickness; IPS e.max CAD, Ivoclar AG) were prepared, alongside zirconia abutments (<i>Ø</i> = 10 mm, 3 mm thickness, 2.5 mm of screw-access hole diameter; IPS e.max ZirCAD MO, Ivoclar AG). The specimens were randomly assigned to five groups based on the thickness of sealing resin composite (Tetric N-Ceram Bulk fill, Ivoclar AG): Ctrl (only PTFE tape); PTFE tape +0.5 mm composite; PTFE tape +1.0 mm composite; PTFE tape +1.5 mm composite; and PTFE tape +2.0 mm composite. Surface treatments were conducted on ceramics before luting with dual-cure resin cement (Multilink N, Ivoclar AG). Monotonic testing was conducted at a loading rate of 1.0 mm/min until crack detection (<i>n</i> = 5). Cyclic fatigue testing was performed (<i>n</i> = 15; 100 N for 5000 cycles, followed by increments of 100 N every 10,000 cycles at 20 Hz) until failure. Finite element and Scanning Electron Microscopy analyses were also performed. One-way ANOVA and Tukey post hoc tests were used for monotonic data, while Kaplan–Meier and Mantel-Cox tests assessed survival rates (<i>α</i> = 0.05) based on fatigue test. No significant differences in monotonic tests were found. However, the 1.5 mm and 2.0 mm groups exhibited significantly higher fatigue failure loads compared to the Ctrl, 0.5 mm, and 1.0 mm groups (0.5 mm: 1093 N = Ctrl: 1120 N = 1.0 mm: 1127 N &lt; 1.5 mm: 1426 N = 2.0 mm: 1307 N, <i>p</i> ≤ 0.05). To improve the fatigue behavior of lithium disilicate restorations bonded to zirconia abutments, more than half of the screw-access hole (greater than 1.5 mm) should be filled with resin composite.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35657","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Application of 3D-Printed Patient-Specific Instruments in Nasal Bone Fracture Reduction: A Randomized Controlled Trial","authors":"Yuting Ge,&nbsp;Chenjie Xu,&nbsp;Peihua Wang","doi":"10.1002/jbm.b.35655","DOIUrl":"https://doi.org/10.1002/jbm.b.35655","url":null,"abstract":"<div>\u0000 \u0000 <p>To investigate the efficacy of a 3D-printed reduction instrument for nasal bone reduction, comparing it with conventional Boies elevators. A prospective, randomized, controlled trial was conducted on 78 patients with nasal bone fractures at Shanghai Ninth People's Hospital (2017–2020). Patients were assigned to either the 3D group (3D-printed reduction instrument, <i>n</i> = 39) or the BE group (Boies elevators, <i>n</i> = 39). Preoperative and postoperative nasal alignment and fracture parameters were analyzed using Mimics software, while patient satisfaction was assessed via a visual analog scale (VAS). Postoperative condition was graded using CT imaging. Statistical comparisons were performed using <i>t</i>-tests, Mann–Whitney U tests, and generalized estimating equations. Both groups showed significant improvements in fracture displacement (BE: 1.94–1.12 mm, 3D: 1.93–0.90 mm, <i>p</i> = 0.000), fracture angle (BE: 151.79°–169.91°, 3D: 149.34°–177.47°, <i>p</i> = 0.000), and midline displacement (BE: 7.79–4.42 mm, 3D: 7.44–0.00 mm, <i>p</i> = 0.000). The 3D group demonstrated significantly better postoperative midline displacement (<i>p</i> = 0.007), fracture angle (<i>p</i> = 0.003), and overall postoperative condition (<i>p</i> = 0.029). However, there was no significant difference in postoperative patient satisfaction scores (nasal obstruction: <i>p</i> = 0.053; nasal appearance: <i>p</i> = 0.318). The 3D-printed reduction instrument provides superior anatomical reduction and postoperative outcomes compared to Boies elevators. While both techniques effectively improve nasal alignment, patient satisfaction did not significantly differ. The 3D instrument offers a promising alternative when precision is required in nasal bone reduction.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Treatment With Cell Culture Medium: A Biomimetic Approach to Enhance the Resistance to Biocorrosion in Mg and Mg-Based Alloys—A Review","authors":"Vida Khalili,&nbsp;Sannakaisa Virtanen,&nbsp;Aldo R. Boccaccini","doi":"10.1002/jbm.b.35617","DOIUrl":"https://doi.org/10.1002/jbm.b.35617","url":null,"abstract":"<p>In contemporary orthopedics, the demand for temporary biodegradable bone implants has driven the development of materials capable of supporting bone regeneration while gradually resorbing in the body, thereby eliminating the need for secondary removal surgery. Magnesium (Mg) and its alloys have emerged as promising candidates due to their bioactivity, osteoconductivity, and mechanical properties that closely match those of natural bone. Furthermore, the release of Mg²⁺ ions during degradation has been shown to stimulate osteoblast activity and enhance bone remodeling. Despite the advantages associated with Mg as a bone implant, there are also constraints on its clinical application. The elevated pH values inherent to the Mg corrosion process may adversely affect biocompatibility, in addition to general concerns about the burst release of H₂ gas that originates from the cathodic reaction of Mg corrosion. To address these challenges, biomimetic surface modifications have emerged as a promising strategy to modulate the degradation behavior of Mg and its alloys. In particular, Dulbecco's Modified Eagle Medium (DMEM) cell culture medium serves as an effective biomimetic environment for forming corrosion-resistant layers on Mg-based implants, maintaining physiological pH and mimicking in vivo degradation behavior by facilitating the formation of a carbonated Ca/Mg-phosphate layer with superior resistance to Cl⁻ attack compared to Mg(OH)₂. Immersion in DMEM has been shown to induce the formation of calcium phosphate rich protective layers that mimic the natural bone environment and mitigate the rapid biodegradation of Mg and its alloys. This paper provides a review of recent advancements in DMEM modification of Mg-based alloys, including ex situ and in situ formation of protective layers, and in vitro biocorrosion behavior in cell culture medium. Key findings emphasize that synthetic buffers like Tris/HCl and HEPES accelerate corrosion and hinder calcium phosphate formation, while protein-rich media risk contamination during prolonged use. Additionally, electrostatic interactions in DMEM promote hydroxyapatite crystallization, functionalized intermediate layers enhance calcium phosphate deposition, and fluid dynamics must be carefully controlled to stabilize the protective layer. Despite recent progress, key knowledge gaps remain, including limited understanding of the long-term performance and mechanical stability of biomimetic layers under dynamic physiological conditions, as well as the unclear impact of complex in vivo factors like immune responses and enzymatic activity on their degradation.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35617","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene Oxide-Based Photothermal and Photodynamic Therapy—A Systematic Review","authors":"Shahrukh Khan,&nbsp;Humaira Batool,&nbsp;Huraira Tariq,&nbsp;Aneeqa Noor","doi":"10.1002/jbm.b.35656","DOIUrl":"https://doi.org/10.1002/jbm.b.35656","url":null,"abstract":"<div>\u0000 \u0000 <p>Photothermal therapy and photodynamic therapy are two advanced strategies used in modern medicine that rely on the use of innovative materials with high photothermal abilities. As graphene oxide and reduced graphene oxide possess a unique ability to respond to near-infrared light in a broadband range and efficiently convert it into heat, they have proved to be ideal nanomaterials to engineer efficient and multifunctional photothermal agents. A lot of research has been done to fabricate efficient graphene oxide-based photothermal platforms that can be used for photothermal and photodynamic therapy. The practicality of a number of these agents has been tested in various biomedical applications, mostly using antimicrobial and anticancer models, both in vitro and in vivo. In this review, we systematically analyzed all the studies published in the past decade on graphene-based photothermal nanosystems tested for effective use in phototherapies/combined therapies in various biomedical applications. The search strategy involved the use of specific keywords and Boolean operators and was limited by the full-text availability of articles on PubMed. This review outlines the design of various graphene-based photothermal platforms, their effectiveness in enhancing therapeutic outcomes, and their limitations that pose a hurdle in the standardization and clinical translation of these platforms. Moreover, through a critical analysis of persisting gaps in current designs, this review can assist in guiding researchers to devise an ideal multifunctional nanosystem for phototherapy that combines the effective properties of different agents and overcomes the shortcomings of existing platforms.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osseointegration of Nitrogen-Containing Bisphosphonate Coatings on Dental Implants: An Experimental Pilot Study","authors":"Reza Ardeshir Mokhtari,&nbsp;Marcel Kunrath,&nbsp;Christelle Darnaud,&nbsp;Hanna Aludden,&nbsp;Lotta Tollstoy,&nbsp;Anders Palmquist,&nbsp;Christer Dahlin","doi":"10.1002/jbm.b.35653","DOIUrl":"https://doi.org/10.1002/jbm.b.35653","url":null,"abstract":"<p>To compare the osseointegration properties of two different types of nitrogen-containing bisphosphonate (N-BP)-coated dental implants in an experimental in vivo sheep model. In total, eight sheep were divided into two groups receiving implants at two time points. Each animal received four types of implants, AddBIO STL implants coated with Zoledronate (Zol) and AddBIO STL implants coated with Ibandronate and Pamidronate (IbaPam) as test groups and uncoated AddBIO STL implants (UC) and Straumann original SLA implants (SSLA) as controls. Implants were placed in the metatarsus bilaterally, and healing times were either 10 or 28 days. Implant stability quotient (ISQ) was measured at baseline and at euthanasia. Removal torque (RT) was assessed post-mortem, followed by histomorphometric analysis to evaluate bone-to-implant contact (BIC) and bone area (BA). The differences between implants were evaluated using paired t-tests. The significance level was set at <i>p</i> value &lt; 0.05 After 10 days, Zol implants presented significantly higher RT (Ncm mean ± SD) values 26.0 (± 18.0) than IbaPam implants 8.3 (± 14.9) (<i>p</i> = 0.011). SSLA implants demonstrated significantly higher RT values 34.6 (± 22.2) than Zol and IbaPam-coated implants (<i>p</i> = 0.048) and Zol-coated implants showed significantly higher RT values than UC implants 12.0 (± 7.6) than UC implants (<i>p</i> = 0.015). No significant differences in RT were detected at 28 days. No differences were observed among the groups regarding ISQ, BIC, or BA at either time point. Zoledronate-coated implants exhibited enhanced early mechanical stability compared to ibandronate/pamidronate-coated implants. However, this advantage was eradicated after 28 days, suggesting that the early anabolic effect of zoledronate may be time-limited.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35653","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Characterization of a Sardine Scale-Chitosan Biomaterial: In Vivo Evaluation for Bone Regeneration in a Rat Osteoporosis Model","authors":"Nada Hamrouni,&nbsp;Hassane Oudadesse,&nbsp;Bertrand Lefeuvre,&nbsp;Elodie Bouvret,&nbsp;Sterenn Le Penven","doi":"10.1002/jbm.b.35620","DOIUrl":"https://doi.org/10.1002/jbm.b.35620","url":null,"abstract":"<p>The development of functional materials for osteoporosis is essential for effective bone remodeling. In this context, the extraction of biocompatible implantable biomaterials from bio-waste emerges as a valuable strategy, addressing both environmental challenges and promoting human health. The objective of this work was to evaluate the physicochemical properties of the added-value by-product biomaterial (SS-90), extracted from sardine scales (<i>Sardina Pilchardus</i>) and combined with chitosan (SS-90-CH). Besides, the efficacy of both biomaterials for bone regeneration was evaluated through in vitro and in vivo tests. The physicochemical characteristics of the biomaterials were demonstrated by ICP-OES, TGA, XRD, FTIR, and SEM-EDS analyses. Their characteristic features were compared with pure commercial hydroxyapatite (HA_syn) and associated with chitosan (HA_syn-CH). ICP-OES analysis evidenced the presence of Ca, P, Mg, Na, Sr, and Zn in SS-90 with a molar ratio (Ca/P) of 1.84 near to that of synthetic hydroxyapatite (1.67). The FTIR spectrum confirmed the presence of carbonate and phosphate functional groups in SS-90, which is similar to healthy rat bone (HRB). In vitro, SS-90 and SS-90-CH biomaterials demonstrated no cytotoxicity, maintaining cell viability between 80% and 100% for SaOS-2, L929, and LIG cells after 72 h of incubation. Furthermore, these biomaterials were implanted into bone defects in femoral condyles of osteoporotic rats to evaluate their effectiveness in bone fracture repair under osteoporotic conditions. Physicochemical, biochemical, and histological studies conducted at different time intervals after implantation indicated that the biomaterials could effectively promote bone regeneration. In conclusion, the present study highlights that SS-90 and SS-90-CH biomaterials are promising solutions for repairing bone defects or fractures under osteoporotic conditions, combining the valorization of marine bio-waste with biomedical applications.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35620","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composite Scaffolds of Decellularized Placenta/PRP Enhance the Differentiation of Adipose Tissue-Derived Mesenchymal Stem Cells Into Insulin-Producing Cells In Vitro","authors":"Azam Bozorgi,&nbsp;Maryam Bozorgi,&nbsp;Leila Rezakhani,&nbsp;Mozafar Khazaei","doi":"10.1002/jbm.b.35639","DOIUrl":"https://doi.org/10.1002/jbm.b.35639","url":null,"abstract":"<div>\u0000 \u0000 <p>In the current in vitro experiment, we fabricated and characterized placenta/platelet-rich plasma (PL/Pt) composite scaffolds and evaluated their effect on differentiating adipose stem cells (ASCs) into insulin-producing cells (IPCs) in vitro. The human placenta (PL) was decellularized (dPL), characterized, and digested in pepsin. PRP was extracted using a two-step centrifugation process and then freeze-dried. PL/Pt composite scaffolds were fabricated from PL with various PRP concentrations (1, 2.5, and 5 mg·mL) and characterized. ASCs were isolated from adipose tissue and characterized using flow cytometry and multilineage differentiation assays. ASCs were seeded onto PL/Pt scaffolds and differentiated into IPCs. The decellularized placenta retained its collagen content and had minimal cellular and DNA content. ASCs expressed CD73, CD90, and CD105 but not CD34 and CD45 and differentiated into osteoblasts and adipocytes. PL/Pt scaffolds showed appropriate morphological and chemical properties with improved porosity, swelling, and degradation rates. Seeding cells on the PL scaffolds increased PDX1, GLUT2, and insulin expression significantly compared to cells cultured in plates. Cells seeded on PL/Pt2.5 and PL/Pt5 scaffolds showed a remarkable increase in PDX1, GLUT2, and insulin expression. Additionally, differentiated cells cultivated on PL/Pt scaffolds exhibited enhanced insulin and C-peptide secretion in response to variations in glucose levels. PL/Pt composite scaffolds provide a biomimetic microenvironment supporting ASCs' survival while enhancing their differentiation into IPCs. This approach could be a promising strategy for replacing the damaged <i>β</i> cell population in diabetic patients.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}