Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Decellularised Amniotic Membrane for the Neurogenic Expression of Human Mesenchymal Stem Cells","authors":"Jingwen Wu,&nbsp;Yantong Wang,&nbsp;Tong Zhang,&nbsp;Fenglin Yu,&nbsp;Yunci Wang,&nbsp;Xiaoyong Ran,&nbsp;Qi Hao,&nbsp;Yangyang Cao,&nbsp;Yanchuan Guo","doi":"10.1002/jbm.b.35588","DOIUrl":"https://doi.org/10.1002/jbm.b.35588","url":null,"abstract":"<div>\u0000 \u0000 <p>To observe the induction of neurogenic differentiation in human mesenchymal stem cells (hMSCs) by decellularized amniotic membrane (DAM), thereby promoting neural regeneration for peripheral neuropathy. Subcutaneous implantation and immunofluorescence staining were conducted to observe the condition of neural cells. Cell adhesion and viability were evaluated through adhesion assays and live/dead cell staining on the DAM. Spatial transcriptomics sequencing was performed to analyze the expression of genes related to adhesion and neural differentiation. Subsequently, stem cells were seeded onto the DAM, and immunofluorescence staining was used to observe neural cell markers and cell migration capabilities. Finally, a network pharmacological analysis, based on the spatial transcriptome results, was performed to identify neurological-related disorders that may be treated by DAM. The cell adhesion assays showed an increased number of adherent cells with normal morphology. Spatial transcriptomics analysis indicated that the DAM significantly upregulated genes associated with cell adhesion and neural differentiation. Immunofluorescence staining revealed that the DAM significantly induced the expression of neural marker proteins. Lastly, subcutaneous implantation demonstrated the aggregation of neural-related cells. DAM can promote stem cell adhesion, induce cell migration, and thereby enhance neural repair and regeneration in cases of peripheral neuropathy.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and Efficacy of S53P4 Bioactive Glass in Osteomyelitis Management: A Systematic Review and Meta-Analysis","authors":"Antoinette T. Nguyen,&nbsp;Rena A. Li,&nbsp;Robert D. Galiano","doi":"10.1002/jbm.b.35597","DOIUrl":"https://doi.org/10.1002/jbm.b.35597","url":null,"abstract":"<p>Osteomyelitis remains a difficult-to-treat bone infection due to its high recurrence risk, complex surgical demands, and rising rates of multidrug-resistant organisms. While conventional treatments rely heavily on antibiotic-loaded materials, bioactive glass S53P4 offers a dual-action alternative, combining bacteriostatic and bactericidal activity with bone regenerative potential. A systematic review and meta-analysis following PRISMA guidelines was conducted to evaluate the clinical efficacy and safety of S53P4 bioactive glass in osteomyelitis treatment. Twenty-eight studies published between 2000 and 2024, encompassing 1122 patients (mean age: 43.6 years), were included. Outcomes analyzed included infection eradication, recurrence, bone healing, functional recovery, and complications. The risk of bias was assessed using ROBINS-I for observational studies and the JBI checklist for case series. A meta-analysis of 10 studies reporting infection eradication at ≥ 12 months was performed using a random-effects model. The pooled infection eradication rate was 88.1% (95% CI: 85.4%–90.4%) with no significant heterogeneity (<i>I</i>² = 0%). Studies reported consistent efficacy across chronic, diabetic foot, mastoid, and jaw osteomyelitis. S53P4 was effective against polymicrobial and multidrug-resistant infections, including <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>. Healing outcomes were favorable, with high rates of bone integration and return to function. Complications were uncommon and primarily related to soft tissue coverage. Most patients received systemic antibiotics; no studies required local antibiotic-loaded materials alongside S53P4. Bioactive glass S53P4 is a safe and effective adjunct in osteomyelitis management, demonstrating high long-term infection control, robust bone regeneration, and a low complication profile. Its nonantibiotic antimicrobial mechanism makes it particularly suitable in settings of antimicrobial resistance. Future studies should assess its long-term durability and applications in high-risk infections.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35597","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Assessment of Internal and Marginal Fit of Provisional Crowns Fabricated Using 3D-Printing Technology","authors":"Amanda Endres Willers,&nbsp;Marcelo Giannini,&nbsp;Ronaldo Hirata,&nbsp;Edmara T. P. Bergamo,&nbsp;Beatriz de Cássia Romano,&nbsp;Carolina Bosso André,&nbsp;Pablo J. Atria,&nbsp;Lukasz Witek","doi":"10.1002/jbm.b.35595","DOIUrl":"https://doi.org/10.1002/jbm.b.35595","url":null,"abstract":"<div>\u0000 \u0000 <p>To evaluate the effect of silanized filler particles and blue light-sensitive photoinitiator system on the internal and marginal fit of 3D printed resin crowns as well as the volume of provisional cement space. This study evaluated three commercially available 3D-printing resins (Smart Print Temp/SP, Resilab 3D Temp/RL, and Cosmos Temp/CT). The experimental groups consisted of the addition of 30% by weight (30 wt%) of silanized filler particles and a blue light-sensitive Ternary Photoinitiator System (TPS). Samples were printed for each group (<i>n</i> = 10) and evaluated for internal and marginal fit and volume of cement space using a micro-computed tomography (μCT). The obtained data were analyzed by Generalized Linear Models (<i>α</i> = 0.05). The incorporation of TPS and filler particles to the 3D printing resins altered the internal fit and the marginal fit, increasing the cement space at the occlusal face and decreasing the cement space of the axial walls for all tested materials. The volume was significantly affected too, especially for RL and CT. Internal misfit was significantly higher with the addition of TPS, and marginal misfit with the addition of filler particles. In general, the incorporation of TPS and 30 wt% of filler particles promoted an increase in the volume of cement space, as well as an increase in occlusal space and marginal space and a decrease in the axial walls' spaces.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a New Curcumin-Loaded Dental Varnish for Antimicrobial Photodynamic Therapy Application","authors":"Patricia Comeau,&nbsp;Maria Luisa Leite,&nbsp;Adriana Manso","doi":"10.1002/jbm.b.35596","DOIUrl":"https://doi.org/10.1002/jbm.b.35596","url":null,"abstract":"<p>The main objective of this study was to develop a natural curcumin-loaded varnish that responds to blue light in an antimicrobial photodynamic therapy-based approach with photoelimination of targeted cells. Fluoride-free curcumin-loaded varnish (CUR-V) consisting of gum rosin (80 wt%) and ethanol (20 wt%) was loaded with 0, 0.25, and 10.0 wt% curcumin and then analyzed with FTIR. Subsequent investigation included applying ~19 mW/cm² blue light for 15, 30, or 60 min (in order of increasing energy dose) to the varnishes and measuring the change in varnish color and the production of reactive oxygen species (singlet oxygen and superoxide anion), as well as performing CFU count and XTT assays of 24-h mono-species biofilms of <i>Streptococcus mutans</i> and <i>Candida albicans</i>. Lastly, a 72-h dual-species biofilm of both species was assessed with a CFU count assay. As the light energy applied to the 0.25% CUR-V and 0.00% CUR-V samples increased, there was a reduced and quantifiable difference in color between these two varnish groups (<i>p</i> &lt; 0.001). In addition, singlet oxygen production was most dependent on curcumin loading in the varnish, while superoxide anion production appeared more dependent on blue light energy (<i>p</i> &lt; 0.001 for both). In subsequent investigations of mono- and dual-species biofilms, the ability to induce an agonistic antimicrobial response as a function of curcumin loading in the natural varnish and blue light energy was demonstrated. Inherently, as curcumin loading and blue light energy increased, the photoelimination of both species investigated increased. As expected, the impact of aPDT on the 72-h biofilm was less than that observed for the 24-h biofilm; however, the more mature dual-species biofilm still observed a photoelimination effect with greater curcumin concentration. This study provides early evidence toward the development of a new natural curcumin-loaded fluoride-free varnish that, when combined with blue light irradiation, shows significant potential to effectively provide a targeted antimicrobial response against cariogenic species. To date, there is no similar approach that can serve as a natural, fluoride-free over-the-counter alternative to assist in caries prevention and treatment.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35596","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium Phosphate Apatite Filament Co-Wrapped With Perforated Electrospun Sheet of Phosphorylated Chitosan—A Bioinspired Approach Toward Bone Graft Substitute","authors":"Prabhash Dadhich,&nbsp;Pallabi Pal,&nbsp;Nantu Dogra,&nbsp;Pavan K. Srivas,&nbsp;Bodhisatwa Das,&nbsp;Samir Das,&nbsp;Pallab Datta,&nbsp;Baisakhee Saha,&nbsp;Bo Su,&nbsp;Santanu Dhara","doi":"10.1002/jbm.b.35589","DOIUrl":"https://doi.org/10.1002/jbm.b.35589","url":null,"abstract":"<div>\u0000 \u0000 <p>Bioinspired bone graft substitutes hold incredible opportunities in tissue engineering, potentiating the healing aspect. Here we have fabricated stacks of glutaraldehyde–genipin crosslinked, microporous nanofibrous <i>N</i>-methyl phosphonic chitosan sheets (NMPC) with impregnated eggshell-derived CaP fibers to mimic osteonal architecture. This composite 3D rolled eggshell-derived calcium phosphate (ESCAP) scaffold (RCS), with density and modulus variation from the center to the periphery, has superior mechanical strength. The zwitterionic nature of NMPC, following the surface modulus of the CaP fibers, upgraded the biological performance. The low modulus of the flexible micro-perforated nanofibrous sheet increases along the ceramic phase, which prompts migration and distribution of proliferated MSCs from the outer polymeric surface to the inner ceramic region through micro-perforations. This movement stimulates endochondral ossification, observed by a gradual increment of collagen II expression alongside a decrement of collagen I expression. In vivo assessment of rabbit tibia bone defects revealed prominent healing in the presence of a scaffold by Day 60, accompanied by scaffold resorption. The cellular activity during healing revealed osteoblasts, osteocytes, blood vessels, and chondroblast cells at the boundary of the scaffolds, indicating neotissue and hypertrophic cartilage formation. Thus, the RCS bone grafts promote faster bone healing by osteogenesis and bone remodeling.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carrageenan-Based Hydrogels for Advanced Wound Healing and Controlled Drug Delivery in Tissue Engineering","authors":"Great Iruoghene Edo,&nbsp;Winifred Ndudi,&nbsp;Raghda S. Makia,&nbsp;Irene Ebosereme Ainyanbhor,&nbsp;Emad Yousif,&nbsp;Tayser Sumer Gaaz,&nbsp;Endurance Fegor Isoje,&nbsp;Rapheal Ajiri Opiti,&nbsp;Patrick Othuke Akpoghelie,&nbsp;Ufuoma Augustina Igbuku,&nbsp;Dina S. Ahmed,&nbsp;Arthur Efeoghene Athan Essaghah,&nbsp;Huzaifa Umar","doi":"10.1002/jbm.b.35594","DOIUrl":"https://doi.org/10.1002/jbm.b.35594","url":null,"abstract":"<div>\u0000 \u0000 <p>Carrageenan (CGN) is a high molecular weight polysaccharide that is extracted from red seaweeds. It is made up of D-galactose residues connected by β-1,4 and α-1,3 galactose-galactose bonds. As a result of its ability to thicken, emulsify, and stabilize food, it is frequently used as a food additive in processed food. Its consumption has surged in recent years due to the Western diet's (WD) spread. Carrageenan has the ability to change the thickness of the mucus barrier, the composition of the gut microbiota, and the innate immune pathway that causes inflammation. Also, its inherent qualities, which include biodegradability, biocompatibility, resemblance to native glycosaminoglycans, antioxidants, anticancer, immunomodulatory, and anticoagulant activities, Carrageenan-based hydrogels have been the subject of numerous investigations lately for biomedical applications. The brittle hydrogel and uncontrollably exchanged ions, however, are two drawbacks to the application of this polysaccharide, but these can be avoided by making straightforward chemical changes to polymer networks, which create chemically bonded hydrogels with important mechanical characteristics and regulated degradation rates. Furthermore, the addition of diverse kinds of nanoparticles, as well as polymer networks, to carrageenan hydrogels results in hybrid platforms with noteworthy mechanical, chemical, and biological characteristics, which qualify them as appropriate biomaterials for tissue engineering (TE), drug delivery (DD), and also wound healing applications. Our goal in this article is to provide an overview of the most current developments in hybrid carrageenan-based platforms and several chemical modification techniques for TE and DD applications.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143949955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Degradation and Cytocompatibility Study of Biodegradable Porous Zinc Scaffolds Fabricated by Fused Deposition Modeling Based Rapid Tooling Method","authors":"Abhishek Kansal,&nbsp;Sangita Mahapatra,&nbsp;Akshay Dvivedi,&nbsp;Pradeep Kumar,&nbsp;Mayank Goswami","doi":"10.1002/jbm.b.35593","DOIUrl":"https://doi.org/10.1002/jbm.b.35593","url":null,"abstract":"<div>\u0000 \u0000 <p>An integrative study is conducted on the static biodegradation behavior and cytocompatibility of organized porous network structured (OPNS) zinc scaffolds fabricated via the Fused Deposition Modeling based Rapid Tooling (FDM-RT) method. The degradation study investigates the corrosion mechanism over immersion time in simulated body fluid (SBF), whereas the cytocompatibility study incorporates MTT assay and direct cell counting tests. The results indicate that the scaffold morphology, including scaffold struts, interconnectivity, or porosity, along with the immersion period, significantly influence the degradation behavior. The static corrosion rates are determined to be 0.27 ± 0.006, 0.79 ± 0.01, and 1.35 ± 0.05 mm y⁻¹ for bulk and porous zinc samples after 28 days. The higher corrosion rate of porous zinc alloys is mainly due to their higher surface area, which enhances exposure to the solution. Over time, protective layers form on both porous and bulk samples, leading to a gradual decrease in corrosion rate. The XRD results reveal that the corrosion protective layer is primarily composed of ZnO, Ca₃(PO₄)₂, Zn (OH)₂, and Zn₃(PO₄)₂·4H₂O. Moreover, MTT assay and direct cell counting of mouse fibroblast 3T3 cells on bulk and porous zinc samples suggest that the prepared zinc samples have potential for tissue engineering applications.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143939408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Collagen and Polylactic Acid Multifilament Yarns for Developing Textile Scaffolds for Tendon Tissue Engineering Applications","authors":"Yihan Huang,&nbsp;Robert Tonndorf,&nbsp;Jessica M. Gluck,&nbsp;Edwin R. Cadet,&nbsp;Martin W. King,&nbsp;Jacqueline H. Cole","doi":"10.1002/jbm.b.35583","DOIUrl":"https://doi.org/10.1002/jbm.b.35583","url":null,"abstract":"<p>This study is the first to compare the mechanical properties and biocompatibility of multifilament yarns made from a natural biopolymer, collagen, and from a synthetic polymer, polylactic acid (PLA), with a view toward evaluating the suitability of these two yarns for fabricating tendon tissue engineering scaffolds. Genipin, the natural plant-based anti-inflammatory and antioxidant crosslinking agent, was used to stabilize the collagen structure. A range of different genipin concentrations, crosslinking temperatures, and crosslinking times were studied, and based on higher degrees of crosslinking, reduced swelling ratio, and greater tensile strength, the recommended conditions are crosslinking the wet-spun collagen yarns with 1.0% genipin at 37°C for 72 h. During a 6-week enzymatic degradation study, the PLA yarns were more resistant to degradation and retained greater tensile strength than the genipin-crosslinked collagen yarns under both dry (4.0 ± 0.2 N vs. 2.2 ± 0.4 N) and hydrated (3.7 ± 0.3 N vs. 0.4 ± 0.1 N) conditions. After coating the PLA yarns with collagen, their biocompatibility improved, and they were able to support tendon cell growth and proliferation. In conclusion, the selection of multifilament PLA yarns coated with collagen is recommended as a promising scaffold material for tendon tissue engineering applications.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35583","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Versatile Biomaterial Additive: A Game-Changing Multifunctional Synthetic Peptide With Pro-Regenerative, Anti-Inflammatory, and Antibacterial Properties","authors":"Mirosława Panasiuk,&nbsp;Milena Chraniuk,&nbsp;Piotr Bollin,&nbsp;Justyna Sawicka,&nbsp;Anna Sylla,&nbsp;Lilit Hovhannisyan,&nbsp;Monika Biernat,&nbsp;Sylwia Rodziewicz-Motowidło,&nbsp;Beata Gromadzka","doi":"10.1002/jbm.b.35591","DOIUrl":"https://doi.org/10.1002/jbm.b.35591","url":null,"abstract":"<div>\u0000 \u0000 <p>The prevalence of bone disorders and deformities is increasing due to trauma, malignant neoplasms, infections, and degenerative and inflammatory diseases. Bone repair and replacement have evolved as a consequence of advancements in orthopedic technology and biomaterials with enhanced properties. The rapid growth of the bone tissue engineering field is being significantly influenced by biomaterials, such as polymer scaffolds with appropriate surface modifications. New additives are constantly being developed in response to the increasing demand for enhancing the bioactivity of biocomposites used for bone regeneration. We present the design and synthesis of a synthetic bioactive peptide UG46, which is multifunctional and consists of the fragment of human Cystatin C (CystC) and anoplin. In addition, the peptide is assessed as an additive that is employed to enhance the repair of bone by enriching porous chitosan (CH) scaffolds. Our results indicated that the UG46 peptide possesses pro-regenerative properties, while it did not exhibit any cytotoxic effects on human osteoblasts or human fibroblasts. Incubation of cells with CH and CH-UG46 extracts significantly increased cell proliferation, cell proliferation over 200% of control cells. Even though the proliferation assay revealed a significant inhibition of cell proliferation in cells seeded directly on the composites, the beneficial effect of the UG46 peptide was still noticeable. Additionally, the UG46 peptide exhibited dose-dependent anti-inflammatory properties in both its free form and as a biocomposite additive, qualifying it as a promising candidate for a bone biomaterial component. The working concentrations of UG46 peptide have been established at 40–80 μg/mL. The molecular structure analysis of the CH-UG46 biocomposites revealed that the majority of the pores were sufficiently large to enable osteoblast cells to infiltrate the scaffold, while concurrent microporosity (&lt; 20 μm) enabled cell infiltration, vascularization, and cell-matrix interactions. Additionally, the peptide alone exhibits limited antibacterial properties; however, the peptide released from the CH-UG46 biocomposite at high levels has been demonstrated to be capable of inhibiting the growth of the selected bacterial strains that are most frequently found infecting healing wounds by over 83% for all strains tested stains with A. baumani bacterial count reduction at 99.86%. Our findings indicate that the bioactive peptide we have proposed is a promising enhancement for porous scaffolds. It has the potential to facilitate the creation of specialized, custom-designed biomaterials with multifunctional properties, including healing, defense, and hygiene.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-Pot Synthesis of Antibacterial and Antioxidant Self-Healing Bioadhesives Using Ugi Four-Component Reactions","authors":"Ronak Afshari,&nbsp;Arpita Roy,&nbsp;Saumya Jain,&nbsp;Kaimana Lum,&nbsp;Joyce Huang,&nbsp;Sam Denton,&nbsp;Nasim Annabi","doi":"10.1002/jbm.b.35584","DOIUrl":"https://doi.org/10.1002/jbm.b.35584","url":null,"abstract":"<div>\u0000 \u0000 <p>Bioadhesive materials are extensively utilized as alternatives to surgical sutures and wound dressings. Despite significant advancements in their synthesis, current bioadhesives suffer from inadequate mechanical stability, suboptimal wet tissue adhesion, and a lack of inherent antibacterial and antioxidant properties, while requiring multistep synthesis processes, complicating their production for biomedical applications. To address these limitations, we developed a new bioadhesive, named UgiGel, synthesized through a one-pot Ugi four-component reaction (Ugi-4CR). Our strategy utilized gelatin as the backbone, 4-formylphenylboronic acid (4-FPBA) as an aldehyde source for improved adhesion and antibacterial activity, gallic acid (GA) as a carboxylic acid source for improved antioxidant activity and wound healing, and cyclohexyl isocyanide (CyIso) to induce pseudopeptide structures. The internal crosslinking between GA and 4-FPBA via dynamic boronate ester bond formation, triggered by slight pH changes (7.4–7.8) and temperature elevation (25°C–40°C), resulted in the formation of viscoelastic and self-healing hydrogels with water as the only byproduct without the need for initiator/light activation. UgiGel showed higher adhesion to porcine skin tissue (139.8 ± 8.7 kPa) as compared to commercially available bioadhesives, Evicel (26.3 ± 2.6 kPa) and Coseal (19.3 ± 9.9 kPa). It also demonstrated effective antibacterial properties against both Gram-negative and Gram-positive bacteria, as well as antioxidant activity. Additionally, the in vitro studies using NIH-3T3 cells confirmed the biocompatibility of the UgiGel over 7 days of culture. Moreover, in vivo biocompatibility and biodegradation of UgiGel were confirmed via subcutaneous implantation in rats for up to 28 days. Our results demonstrated that UgiGel outperformed commercially available bioadhesives in terms of adhesion, self-healing, and antibacterial activity, without compromising biocompatibility or physical properties, representing a promising multifunctional bioadhesive for wound sealing and repair.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143897117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}