Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Correction to \"Corrosion-Driven Degradation of Mg WE43 Plates for Mandibular Fracture Fixation: An In-Silico Study\".","authors":"","doi":"10.1002/jbm.b.70152","DOIUrl":"https://doi.org/10.1002/jbm.b.70152","url":null,"abstract":"","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 9","pages":"e70152"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alignment of Micrometer-Scale Electrospun Fibers Does Not Enhance Performance of Primary Human Renal Proximal Tubular Epithelial Cells.","authors":"IJsbrand M Vermue, Christian G M van Dijk, Ihsan Chrifi, Ymke Appels, Jurjen van Meijeren, Bastiaan Terhaard, Nahid Kasaiyan, Marianne C Verhaar, Caroline Cheng","doi":"10.1002/jbm.b.70151","DOIUrl":"https://doi.org/10.1002/jbm.b.70151","url":null,"abstract":"<p><p>Renal failure remains a leading cause of morbidity and mortality, as current renal replacement therapies restore filtration but fail to replicate the kidney's transport, metabolic, and endocrine functions. Bioengineered systems using primary renal epithelial cells show promise in recapitulating these processes, and integration into synthetic tubular platforms may further enhance biological mimicry, advancing drug discovery, and next-generation bioartificial kidneys. The native proximal tubule basement membrane contains aligned ridges (~200 nm), suggesting that scaffold fiber alignment could improve epithelial cell function. We therefore hypothesized that aligned electrospun fibers would promote proximal tubule epithelial cell organization and enhance functional performance compared to random fibers. Polycaprolactone scaffolds with random or aligned fibers were fabricated in both flat and tubular configurations using solution electrospinning (SES). Human renal proximal tubule epithelial cells (HRPTEpiC) were cultured for up to 11 days and assessed for morphology, barrier function, polarization, and transporter expression. Aligned fibers induced cytoskeletal organization but did not affect tight junction formation, polarization, or monolayer formation. Barrier function was significantly reduced on aligned scaffolds at Day 11, attributed to larger pore sizes (15.3 vs. 7.5 μm²). Transporter expression showed transient differences, with ATP1A1 and AQP1 reduced on aligned scaffolds at Day 7 but equalized by Day 11. Functional transport assays revealed significant ABC transporter inhibition only on random scaffolds (p = 0.048 vs. p = 0.058 for aligned). Overall, these findings show that at the tested diameters (1.2-1.6 μm), fiber alignment influenced cell morphology but offered limited functional benefit compared to random fibers.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 9","pages":"e70151"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication, In Vitro and In Vivo Evaluation of Bioactive Glass-Doped Biodegradable Bilayer Polymeric Dental Nanofibrous Membranes for Guided Tissue Regeneration Applications.","authors":"Ceren Keçeciler-Emir, Nurettin Diker, Yeşim Müge Şahin, Sevil Yücel","doi":"10.1002/jbm.b.70149","DOIUrl":"https://doi.org/10.1002/jbm.b.70149","url":null,"abstract":"<p><p>Barrier membranes are used in guided bone regeneration (GBR) to prevent the migration of fibroblastic cells into the alveolar bone defects and to ensure the regeneration of bone tissue. The regeneration capabilities of alveolar bone tissue and connective tissue are different, so the niche required by the tissues must also meet this need. In this study, a bilayer synthetic polymeric barrier membrane containing 45S5 bioactive glass (BG) was prepared by the electrospinning method to provide regeneration of both tissues. A fibrous layer was fabricated using polyvinyl alcohol (PVA) containing 3% BG for the soft tissue interface, and a second fibrous layer was fabricated on top of this layer using poly(lactide-co-glycolide) (PLGA) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) blend polymers containing 10% BG for the hard tissue interface. It has been demonstrated that bilayer nanofiber membranes show suitable mechanical, thermal, and physicochemical properties for using GBR applications, as well as high bioactivity and suitable bioresorbable properties. In vitro and in vivo animal study biological assays have demonstrated that nanofibrous multilayer dental membranes are highly biocompatible with fibroblast and bone cells, accelerate cell proliferation, and increase cell adhesion to the membranes. It has been demonstrated that the designed bioactive bilayer nanofiber membranes can meet the requirements for use as a support material in GBR applications in dentistry.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 9","pages":"e70149"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of Mesoporous Manganese Nanoparticles Incorporated Aβ-Peptide Inhibits Aβ Aggregation and Alleviates Neuroinflammation Against Alzheimer's Disease","authors":"Haipeng Lu,&nbsp;Chuanchen Hu,&nbsp;Yaming Fu","doi":"10.1002/jbm.b.70131","DOIUrl":"https://doi.org/10.1002/jbm.b.70131","url":null,"abstract":"<div>\u0000 \u0000 <p>Extracellular amyloid plaques from Aβ accumulation and intracellular neurofibrillary tangles (NFTs) from hyperphosphorylated Tau (p-Tau), both leading to neuronal dysfunction, synapse loss, and cognitive decline. Nonetheless, achieving an effective therapeutic outcome is challenging due to the limited drug bioavailability through the blood–brain barrier (BBB) and the complex microenvironment within the brain. This study proposes MM-TA for the synergistic treatment of AD, utilizing mesoporous manganese (MM) as a nanocarrier to deliver a LK7 (Aβ-inhibiting peptide) and a DAA (amino acid-peptide) that inhibits Tau-related fibrils formation. A biomimetic nanocarrier (MM-LD@4CM termed as MLDC) encapsulated with 4T1 cell membranes (4CM) was developed, inspired by 4T1 cells' ability to facilitate BBB penetration. Following traversal of the BBB, MLDC concurrently prevented Tau phosphorylation and suppressed Aβ aggregation. Furthermore, by leveraging MM's catalase-mimetic properties, MLDC mitigated oxidative stress and altered the microenvironment associated with AD progression. In contrast to the singular therapeutic agent, MLDC ameliorated nerve damage and enhanced cognitive function in AD mice by reducing Aβ oligomers, phosphorylated Tau, and inflammation, thereby providing a synergistic therapeutic approach with significant potential for effective AD treatment.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 9","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metalorganic Titanium Treatment to Change Enamel Optical Properties","authors":"Yara Oweis,&nbsp;Amir Elhadad,&nbsp;Sophia Smith,&nbsp;Qiman Gao,&nbsp;Emily Buck,&nbsp;Mohamed-Amine Mezour,&nbsp;Wenge Jiang,&nbsp;Javier Vargas Balbuena,&nbsp;Sailer S. Dos Santos,&nbsp;Marta Cerruti,&nbsp;Faleh Tamimi","doi":"10.1002/jbm.b.70146","DOIUrl":"https://doi.org/10.1002/jbm.b.70146","url":null,"abstract":"<p>Observational studies have shown that human teeth with higher concentrations of titanium traces have better optical properties. The aim of this study was to investigate how titanium ion infiltration into tooth enamel affects its optical properties by studying the diffusion and reactivity of titanium compounds in tooth enamel and how this affected enamel optical properties. Tooth enamel specimens were incubated in hydrophobic solutions of titanium isopropoxide. The effect of the treatments on surface composition, topography, enamel hardness, shade, and crystallinity were assessed. Exposing enamel to hydrophobic solvents dissolved part of the acquired enamel pellicle proteins and increased the reactivity of the enamel surface. This allowed the penetration of organic titanium ions deep into dental enamel, which increased the tooth whiteness. The findings of this study will set the basis towards new dental therapies based on titanium modification of enamel tooth structure.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 9","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.70146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonlinear Hyperelastic Characterization of the PolyJet Digital Anatomy Material Library and Selection Methodology for Tissue-Mimicking Anatomical Models","authors":"Adam S. Verga,&nbsp;Dhruv Ranjan,&nbsp;Ailey G. Fogel-Bublick,&nbsp;Siddarth Pejavara,&nbsp;Scott J. Hollister","doi":"10.1002/jbm.b.70145","DOIUrl":"https://doi.org/10.1002/jbm.b.70145","url":null,"abstract":"<p>Additive manufacturing of anatomical prototypes increasingly relies on multi-material polymer systems designed to approximate the mechanical behavior of biological tissues for medical training and device testing, providing reproducible patient- and pathology-specific alternatives to cadavers and animal models. This study provides the first systematic experimental nonlinear constitutive characterization of nearly the entire Stratasys Digital Anatomy material library and a reference dataset for predictive modeling of PolyJet-printed prototypes in finite element simulations. The nonlinear elastic responses of 99 Stratasys Digital Anatomy PolyJet materials were characterized in tension, and a framework was developed to identify materials that best match target tissue behavior. Four hyperelastic constitutive models (neo-Hookean, Mooney-Rivlin, Ogden, and Holmes-Mow) and three data consolidation approaches were compared to determine an optimal method for reporting representative model coefficients across multiple specimens. In addition to Mooney-Rivlin coefficients, a model-independent comparison of tangent modulus at 2% strain is provided to enable stiffness-based material selection. Four exemplar tissues were matched to PolyJet materials to demonstrate the framework. Material labels were not always reliable indicators of optimal matches. The Digital Anatomy materials did not reproduce the full stiffness range of tissues, and their near-linear elasticity limited their ability to capture nonlinear tissue behavior across strain levels.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanostructured and Functionalized Calcium Hydroxyapatite Enhances Collagen Production by Human Dermal Fibroblasts Compared With Microstructured Counterparts In Vitro","authors":"Elizabeth Cristina Iseke Bispo,&nbsp;Stefhani Martins de Barcelos,&nbsp;Jonad Logan A. Contarato,&nbsp;Atailson Oliveira da Silva,&nbsp;Rafael Vieira Rocho,&nbsp;Ana Clara Nogueira Brathwaite,&nbsp;Venâncio Alves Amaral,&nbsp;Paula Martins de Oliveira,&nbsp;Maria Victória Souto-Silva,&nbsp;Graziella Anselmo Joanitti,&nbsp;Marcilio Cunha-Filho,&nbsp;Guilherme Martins Gelfuso,&nbsp;Juliana Lott de Carvalho,&nbsp;Felipe Saldanha-Araújo,&nbsp;Taís Gratieri","doi":"10.1002/jbm.b.70144","DOIUrl":"https://doi.org/10.1002/jbm.b.70144","url":null,"abstract":"<p>Calcium hydroxyapatite (CaHA) is a well-established biocompatible material widely used to stimulate collagen formation in soft tissues; however, its clinical use is largely restricted to invasive deep dermal injection due to safety concerns associated with microstructured formulations, such as superficial nodule formation and vascular occlusion. Here, we report the development of a nanostructured, lactose-functionalized CaHA produced by nanospray drying, enabling safer and more versatile delivery strategies. Physicochemical properties were systematically characterized, and biological performance was evaluated in human dermal fibroblasts and co-culture with peripheral blood mononuclear cells (PBMCs). The resulting nanostructured CaHA (nanoCaHA) exhibited a submicron particle size (181.06 ± 23.83 nm), narrow polydispersity (PDI 0.23 ± 0.17), and a positive surface charge (+11.75 ± 3.63 mV), attributed to surface protonation during processing. NanoCaHA showed no cytotoxic, genotoxic, or irritant effects in vitro and induced a shift in fibroblast cell-cycle distribution consistent with enhanced proliferative activity. While both nano- and microCaHA increased total collagen production, nanoCaHA significantly upregulated COL1A1 and COL3A1 mRNA expression, particularly under PBMC co-culture conditions, outperforming a commercially available CaHA formulation. Moreover, nanoCaHA sustained collagen production for up to 5 days after treatment withdrawal. Scanning electron microscopy revealed persistent particulate structures closely associated with fibroblasts after washout, while energy-dispersive X-ray spectroscopy confirmed that these deposits were enriched in calcium and phosphorus, consistent with CaHA composition. Furthermore, skin permeation assays using porcine skin in Saarbrücken diffusion cells showed that the nanoCaHA formulation significantly increased calcium delivery into the skin (34.53 ± 6.64 μg/cm²) compared to endogenous control levels (23.43 ± 3.96 μg/cm²). Collectively, these findings demonstrate that nanoscale engineering of CaHA modulates cell–material interactions, supporting the development of safer superficial dermal and novel topical/transdermal delivery systems for regenerative aesthetics.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.70144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilayered Silica-Core/Chitosan-Coated Nanostructures Co-Delivering Tamoxifen and Quercetin Mitigate LPS-Associated Neurodegenerative and Alzheimer's Disease-Like Alterations","authors":"Bassma H. Elwakil,&nbsp;Aya M. Helal,&nbsp;Mostafa El-Khatib,&nbsp;Amira Abdelnaser,&nbsp;Amira Abd-Elfattah Darwish,&nbsp;Nourhan S. Shehata,&nbsp;Sara H. Akl,&nbsp;Esraa Abdelhamid Moneer,&nbsp;Basant A. Bakr,&nbsp;Abeer El Wakil","doi":"10.1002/jbm.b.70150","DOIUrl":"https://doi.org/10.1002/jbm.b.70150","url":null,"abstract":"<div>\u0000 \u0000 <p>Cholinergic dysfunction, oxidative stress, neuroinflammation, and proteinopathies are among the major hallmarks of Alzheimer's disease (AD). This study aimed to develop and evaluate the therapeutic potential of multilayered silica-based/chitosan-coated nanoparticles (Si@CsNPs) co-loaded with quercetin (QUR) and tamoxifen (TAM) in an LPS-induced neuroinflammatory murine model exhibiting AD-like neuropathological alterations associated with bacterial endotoxin exposure. Molecular docking and target prediction analyses suggested that TAM may exert partial cholinesterase inhibitory activity and potentially interact with muscarinic receptor- and epidermal growth factor receptor (EGFR)-associated pathways, which are implicated in neuroinflammation and oxidative stress regulation. The resulting silica-based/chitosan-coated nanoparticles (Si@CsNPs) co-loaded with QUR and TAM (QUR/TAM-Si@CsNPs) were spherical in shape, exhibited a core-shell architecture, and demonstrated high loading efficiencies for QUR (93.1% ± 2.56) and TAM (84.27 ± 3.66), with a mean particle diameter of approximately 135 nm as determined by TEM. Behavioral assessment using Morris Water Maze revealed that lipopolysaccharide (LPS) administration impaired spatial learning and memory in rats; these effects were significantly ameliorated by QUR/TAM-Si@CsNPs treatment, to a greater extent than Si@CsNPs loaded individually with QUR or TAM. Biochemical, apoptotic, and histopathological analyses further demonstrated that treatment with QUR/TAM-Si@CsNPs normalized acetylcholinesterase activity, Tau protein level, β-amyloid accumulation, pro-inflammatory mediators, and apoptotic indices while preserving hippocampal architecture. Collectively, these findings demonstrate that QUR/TAM-Si@CsNPs exert combined anti-inflammatory and neuroprotective effects, highlighting their promise as a multifunctional nanotherapeutic strategy against LPS-associated neurodegenerative and AD-like neuropathological alterations.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shear-Thinning Composite Hydrogel Incorporating Polycaprolactone (PCL) Short Nanofibers for Cell Therapy Applications","authors":"Akram Jassim Jawad,&nbsp;Christian Heiss,&nbsp;Vlad Stolojan,&nbsp;Paola Campagnolo","doi":"10.1002/jbm.b.70126","DOIUrl":"https://doi.org/10.1002/jbm.b.70126","url":null,"abstract":"<p>Nanofibers-reinforced hydrogels offer improved mechanical properties as compared to classic hydrogels and can potentially better support cell viability and cell therapy outcomes. Here, we optimized polycaprolactone (PCL) short nanofibers production and combined them with a soft hydrogel based on <i>kappa-</i>carrageenan and gelatin type B (kCGb) to perform umbilical cord pericyte (UCP) delivery and promote re-endothelialization of injured blood vessels. Electrospinning of PCL identified 12.5% w/v concentration, 0.5 mL/h flow rate, 21 kV voltage, and 6.8 m/s drum collector speed as optimal parameters for aligned beadless nanofibers. Coaxial PCL/gelatin type A (Ga) fibers were successfully produced with diameters of 180–190 nm (core) and 225–245 nm (shell). The Ga acts as a temporary shell, which was removed during postfabrication processing by washing the nanofibers with deionized water (DIW) at 37°C for 30 min. Mechanical cryostat cutting generated short nanofibers (~75 μm ± 28 μm) with higher yields from coaxial mats (2 × 10⁶ fibers/mL ± 1.6 × 10⁵ fibers/mL) compared to single PCL mats (5 × 10⁴ fibers/mL ± 1.3 × 10⁴ fibers/mL). kCGb hydrogel showed a significant improvement in mechanical properties when reinforced with short PCL nanofibers. The recovery percentage, measured using rheology, increased from 12.1% ± 0.3% to 90.9% ± 0.4% when a small amount (0.05 wt%) of short PCL nanofibers was added. The injection force using clinically relevant needles of 0.5kC2Gb0.05PCL was about ~37 N, and there was no significant reduction in UCPs viability after injection up to 1 week of culture. Injection delivery of kCGbPCL mixed with UCPs (1 × 10⁶ cells/mL) in an ex vivo model of porcine artery injury led to a marked improvement in endothelial coverage from 47.2% ± 19.1% in the injured artery to 76.5% ± 13.2% after cell therapy, with a restoration index of about 89.8% after 1 week of culture. These results represent a further step toward developing physically cross-linked and mechanically stable injectable scaffolds for different applications, such as drug delivery, 3D printing, in vitro modeling, and tissue engineering.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.70126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Modification of PEEK by Zn/Mg Ion Implantation for Improved Osteogenic and Antibacterial Performance","authors":"Shanshan Chu,&nbsp;Wenwen Yuan,&nbsp;Ling Tian,&nbsp;Danfeng Feng,&nbsp;Xu Fang","doi":"10.1002/jbm.b.70148","DOIUrl":"10.1002/jbm.b.70148","url":null,"abstract":"<div>\u0000 \u0000 <p>Because its elastic modulus is closer to that of cortical bone, polyetheretherketone (PEEK) has been considered a potential alternative to titanium for dental implants. However, the biological inertness and lack of intrinsic antibacterial activity of pristine PEEK limit its osseointegration and increase the risk of peri-implant infection. In this study, Zn-, Mg-, and Zn/Mg-implanted PEEK were prepared using a metal vapor vacuum arc ion source, with untreated PEEK as the control. Surface chemical composition, ion release, roughness, and wettability were characterized, and the osteogenic responses of MG-63 cells and antibacterial activity against <i>Staphylococcus aureus</i> and <i>Fusobacterium nucleatum</i> were evaluated. Ion implantation introduced Zn and/or Mg into the near-surface region of PEEK and was accompanied by increased surface roughness and improved hydrophilicity. Mg-containing surfaces, particularly the Zn/Mg group, promoted cell spreading, proliferation, alkaline phosphatase activity, and the expression of osteogenesis-related genes, including <i>ALP</i>, <i>IBSP</i>, and <i>BGLAP.</i> Antibacterial activity was more evident on Zn-containing surfaces, whereas Mg alone showed only limited antibacterial effects. Although Zn alone showed stronger inhibition of <i>Fusobacterium nucleatum</i>, Zn/Mg co-implantation achieved a more favorable overall balance between osteogenic and antibacterial performance. Zn/Mg co-implantation improved osteogenesis-related responses while adding antibacterial activity to PEEK surfaces, suggesting its potential as a coating-free modification strategy for PEEK-based implant materials.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148766319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}