Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Chitosan–Gelatin/Hydroxyapatite Scaffolds With Gelatin Carbon Dots for Application in Bioimages and Photobiostimulation Increase Differentiation of hFOB 1.19 Cells","authors":"Janicy Arantes Carvalho,&nbsp;Cristiano Ceron Jayme,&nbsp;Antonio Claudio Tedesco","doi":"10.1002/jbm.b.35675","DOIUrl":"10.1002/jbm.b.35675","url":null,"abstract":"<div>\u0000 \u0000 <p>Tissue engineering is a new alternative for the recovery from bone injuries. Nanomaterials are often combined with scaffolds to improve structure, bioactivity, stability, adhesion, and compatibility. Carbon dots (CDs), which are fluorescent carbon nanomaterials with diameters of less than 10 nm, are powerful allies. In this study, we aimed to develop chitosan-gelatin/hydroxyapatite scaffolds and CDs for use in bone tissue engineering. First, we developed two types of CDs based on gelatin and heat-treated them at 200°C for 3 h (CDA) or 4 h (CDB). Both systems were characterized, and CDA exhibited better quantum yield and cytotoxic behavior. Therefore, we selected CDA for the scaffolds. Scaffolds without (CG/HA) and with CDA (CG/HA/CDA) displayed suitable porosities and degradation rates. Based on in vitro tests, we observed that the CD-containing scaffolds presented an excellent cell adhesion rate (94–100%), an indirect cytotoxicity viability of approximately 75%, and a direct cytotoxicity viability of at least 100% at all analyzed times (<i>p</i> &lt; 0.05). Furthermore, alkaline phosphatase (ALP) expression suggested the formation of more mature osteoblasts in CG/HA/CDA. Its association with CDA promotes bioactivity, stability, cell adhesion, and compatibility. We also highlighted the ability of CG/HA/CDA to emit fluorescence for monitoring cell growth during tissue regeneration. These results demonstrated that CDA is highly biocompatible and supports cell growth, which can induce bone tissue regeneration and help treat bone diseases.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145212794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vivo Efficacy of an Antibiotic Wound Gel in a Sheep Model of Bone Trauma and Biofilm-Related Infection.","authors":"Annika L Gilmore, Helena Vu, Korinna M Hylen, Jacob Adams, Richard T Epperson, Brooke Kawaguchi, Caroline Garrett, Nicholas N Ashton, Eric Cozzone, Charles A Florek, David A Armbruster, David L Rothberg, Dustin L Williams","doi":"10.1002/jbm.b.35669","DOIUrl":"https://doi.org/10.1002/jbm.b.35669","url":null,"abstract":"<p><p>Traumatic extremity injuries suffer a high probability of infection and often amputation due to contamination and delays in treatment. Military service members are predisposed to injury while engaged in conflict, yet current military adherence to antibiotic administration protocols following traumatic injury is lacking. Moreover, systemic antibiotic prophylaxis might not effectively eradicate biofilm throughout the wound site. Previously, an antibiotic wound gel was created to address current limitations of prophylactic antibiotic treatment in austere environments, particularly the battlefield, by offering a simple solution to control the release of tobramycin over a one-week period. We hypothesized that tobramycin eluted from the gel would effectively manage biofilm-related infection when tested in a large animal model of traumatic long-bone injury. Sheep were either treated with tobramycin-loaded gel or gel alone, and the reduction in bioburden was determined by quantifying tissue and inoculation substrates after a one-week period. Results indicated the wound gel was effective at managing biofilm in this model, with no detectable growth observed in tissues collected from treated animals. Further, the antibiotic-loaded wound gel significantly reduced the severity of the inflammatory response in the surrounding tissue. Biofilm presence was confirmed in scanning electron and light microscopy images of tissues treated with gel alone. Additionally, reactive bone growth, a characteristic of biofilm infection, was consistently observed in all untreated animals but appeared effectively managed in those treated with the antibiotic wound gel. Localized delivery of a broad-spectrum antibiotic from a controlled-release gel can improve adherence to antibiotic administration guidelines and has a greater potential to stabilize biofilm-contaminated wound sites quickly after injury while also mitigating a severe inflammatory response.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":"e35669"},"PeriodicalIF":3.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flexibly Reinforced Polycaprolactone Bioelectrodes for Piezoresistive Sensing via Direct Ink Writing.","authors":"Yongpeng Wu, Jiabin Liu, Ronghan He, Xianwei Wang, Pan Xu, Nan Lin, Swee Hin Teoh, Chao Ma, Zuyong Wang","doi":"10.1002/jbm.b.35670","DOIUrl":"https://doi.org/10.1002/jbm.b.35670","url":null,"abstract":"<p><p>Poly(ε-caprolactone) (PCL) is a biodegradable polyester known for its low melting point and high flexibility, making it ideal for various medical device applications. In this study, we introduce a conductive nanocarbon black-blended PCL as a reinforced ink for fabricating flexible and degradable piezoresistive bioelectrodes. This approach enables the creation of a piezoresistive bioelectrode optimized for precise biomechanical sensing. The bioelectrode exhibits exceptional mechanoelectrical stability under high tension (> 350%), repeated drawing (> 40%, 100 cycles), long-term bending (> 7000 cycles), extrusion (> 10,000 cycles), and torsion (> 90°). When assembled into flexible piezoresistive sensors, the sensor achieves a high sensitivity (2.66 kPa^-1 in the range of 0-3 kPa), along with excellent repeatability and durability (10,000 cycles at 5 N). The sensor has promising applications in human health monitoring, including finger, wrist, and elbow activity tracking, as well as knee joint space sensing for guiding precise surgical operations.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":"e35670"},"PeriodicalIF":3.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature Influence on the Aging of Titanium Surfaces Treated With Cold Atmospheric Plasma: Impact on Physicochemical Properties and Biological Responses.","authors":"Gabriel Moura Martins, Vladimir Galdino Sabino, Jussier de Oliveira Vitoriano, Janine Karla França da Silva Braz, Carlos Augusto Galvao Barboza, Rodrigo Sávio Pessoa, Carlos Eduardo Bezerra de Moura, Clodomiro Alves Júnior","doi":"10.1002/jbm.b.35667","DOIUrl":"https://doi.org/10.1002/jbm.b.35667","url":null,"abstract":"<p><p>Titanium surfaces treated with cold atmospheric plasma exhibit enhanced wettability, cell adhesion, and surface energy; however, these beneficial effects tend to diminish over time due to aging-related changes caused by surface recontamination and the gradual loss of reactive species. This study investigates the influence of surface temperature during cold atmospheric plasma (CAP) treatment on the aging behavior of titanium, with a focus on time-dependent changes in physicochemical properties and biological responses. Titanium samples were treated with CAP at controlled surface temperatures of 40°C, 100°C, and 200°C. Treatment at 100°C and 200°C increased surface roughness, with more rounded peaks observed at 200°C. While wettability initially improved after treatment, it gradually declined over time, with the 200°C-treated samples exhibiting the smallest reduction. Biological assays revealed enhanced cell adhesion on surfaces treated at 100°C and 200°C, with scanning electron microscopy (SEM) showing filopodia formation and cell spreading. The Live/Dead assay confirmed improved cell viability on these surfaces. The AlamarBlue assay indicated that surfaces treated at 40°C and 100°C initially supported the highest cell proliferation, while the 200°C-treated samples maintained the most stable proliferation levels over a 15-day aging period. These findings underscore the impact of surface aging on biomedical device performance, highlighting its influence on the biological response. CAP treatment at 200°C provides durable surface modifications that preserve Ti biocompatibility over time, emphasizing the potential of advanced surface treatments to enhance the longevity and functionality of Ti-based biomedical implants.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":"e35667"},"PeriodicalIF":3.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofabricated Hydrogel Composite of Tragacanth Gum and Chitosan Loaded With Copper Oxide Nanoparticles for Enhanced Cutaneous Wound Regeneration.","authors":"Shahriar Hosseini, Maryam Doostan, Amir Hossein Izadi Nazar, Roghayyeh Vakili-Ghartavol, Hassan Maleki","doi":"10.1002/jbm.b.35668","DOIUrl":"https://doi.org/10.1002/jbm.b.35668","url":null,"abstract":"<p><p>Chronic wounds, often complicated by microbial growth and insufficient regeneration, pose a significant challenge. To address these issues, we developed a hydrogel dressing made from natural polymers, tragacanth gum (TG) and chitosan (CS), incorporated synthesized copper oxide nanoparticles (CuO NPs) to promote wound healing and inhibit microorganisms at the wound site. We synthesized CuO NPs using a reduction method with pomegranate peel extract and analyzed their characteristics. The TG/CS hydrogel was then prepared and loaded with the synthesized NPs, followed by relevant physicochemical analysis. The hydrogel's degradation rate and antibacterial activity were determined, and its effects on cell migration and viability in skin fibroblasts were evaluated using suitable methods. The synthesized CuO NPs showed nanometer dimensions (about 30-50 nm) with a consistent spherical morphology, and compositional analysis confirmed the presence of their constituent elements. The TG/CS hydrogel incorporating CuO NPs displayed a smooth and uniform appearance with a porous structure featuring interconnected micrometer-sized pores. Infrared spectroscopy confirmed the functional groups of the hydrogel components and the presence of the NPs. Moreover, this hydrogel demonstrated high liquid absorption, porosity, and stable degradation over several days. It significantly inhibited the growth of both Gram-positive and Gram-negative bacteria. The hydrogel containing 10 wt% CuO NPs stimulated fibroblast cell growth and, most importantly, accelerated wound healing by inducing cell migration and filling the scratch gap within 48 h. Overall, the natural TG/CS hydrogel containing CuO NPs has a high potential to expedite wound healing as a multifunctional wound dressing.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":"e35668"},"PeriodicalIF":3.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscopic Acid-Induced Degradation and Elemental Release From Thermoformed and 3D-Printed Orthodontic Aligners in a Simulated Gastric Environment","authors":"Piero Antonio Zecca,&nbsp;Eleonora Ivonne Scurati,&nbsp;Francesca Zara,&nbsp;Mario Raspanti,&nbsp;Niccolò Baranzini,&nbsp;Gilberto Binda,&nbsp;Marco Serafin,&nbsp;Alberto Caprioglio,&nbsp;Marina Borgese","doi":"10.1002/jbm.b.35674","DOIUrl":"10.1002/jbm.b.35674","url":null,"abstract":"<p>Clear aligners have revolutionized orthodontic treatment, yet concerns are rising about microplastics (MPs) and nanoplastics (NPs) released from these devices through mechanical wear and chemical degradation. Once ingested, these particles may undergo structural and chemical transformations in the gastrointestinal tract, particularly under acidic gastric conditions. Despite growing environmental and toxicological awareness, the degradation patterns of aligner materials remain largely unexplored. This study evaluated the acid-induced degradation and elemental release of thermoformed (TFA) and direct-printed (DPA) aligners in a simulated gastric environment. TFA (Invisalign SmartTrack) and DPA (Graphy TC-85DAC) samples were exposed to hydrochloric acid (pH 2). Surface acid-induced degradation was monitored using atomic force microscopy (AFM) over 60 min, while elemental release was quantified using inductively coupled plasma mass spectrometry (ICP-MS) following acid digestion on 0.5 M HCl leachates after 7 days. TFA rapidly disintegrated into an amorphous gel, preventing AFM imaging at pH 2. DPA maintained integrity and showed progressive roughening: RMS roughness rose from 10.06 to 10.97 nm (+ 9%; <i>p</i> &lt; 0.001), mean roughness from 7.85 to 8.49 nm (+ 8%; <i>p</i> = 0.002), and maximum height from 68.31 to 76.51 nm (+ 12%; <i>p</i> = 0.038). ICP-MS of digested matrices revealed distinct elemental fingerprints: TFA was dominated by Sn (33.42 mg/kg), K (21.35 mg/kg), and Na (13.34 mg/kg); DPA by Ca (36.63 mg/kg), Na (11.87 mg/kg), and Fe (3.2 mg/kg). In 7-day 0.5 M HCl leachates, TFA released Sb 0.13 and Sn 0.09 mg/kg, whereas DPA showed Sb 0.03 and Sn 0.11 mg/kg; DPA leachates were richer in Ca (7.57 mg/kg) and Fe (1.57 mg/kg). DPA exhibited quantifiably slower acid erosion than TFA and distinct elemental release profiles at longer extraction, supporting greater acid-phase stability of DPA and providing elemental markers to trace aligner-derived particles. The results pertain to Invisalign SmartTrack and Graphy TC-85DAC and should not be generalized to all thermoformed or 3D-printed aligners. These findings emphasize the need for biostable, environmentally safer materials in orthodontics, especially considering the ingestion and systemic distribution of MPs.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35674","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Different Adhesive Systems and Resin Cements on the Push-Out Bond Strength of Fiber Reinforced Posts to Root Dentin","authors":"Maher S. Hajjaj","doi":"10.1002/jbm.b.35662","DOIUrl":"10.1002/jbm.b.35662","url":null,"abstract":"<p>Good bond strength between fiber-reinforced posts (FRPs) and root dentin is essential for successful rehabilitation of endodontically treated teeth. 45 human premolar teeth were divided into three main groups (<i>n</i> = 15) based on bonding agent use: no bonding (control), light-cured (LC), and dual-cured (DC). Each group was further split by cement type: self-adhesive resin cement, bioactive resin cement, and core build-up material, totaling nine subgroups. The teeth were sectioned perpendicularly to the root surface to obtain two middle-root slices. After thermocycling, the push-out bond strength (PBS) test was performed and data were statistically analyzed with the ANOVA test followed by the post hoc Tukey test. Failure modes were examined under a stereomicroscope and statistically evaluated using the <i>χ</i>² test. There was a significant difference in the PBS between test groups (<i>p</i> &lt; 0.0001*). In control groups, core build-up material (Control/LZ = 7.6 ± 3.4 MPa) had significantly lower PBS than the rest of the groups, except Control/AB = 9.9 ± 3.3 MPa. The application of bonding agents significantly increased bond strength for bioactive cement (LC/AB = 14.8 ± 4.8 MPa and DC/AB = 17.7 ± 4.5 MPa) and core build-up material (LC/LZ = 20.4 ± 6.4 MPa and DC/LZ = 16.4 ± 3.8 MPa). Notably, self-adhesive resin cement achieved statistically similar PBS even without the application of bonding agent (Control/RX = 13.6 ± 3.1 MPa, LC/RX = 17.4 ± 5.5 MPa, and DC/RX = 17.0 ± 5.8 MPa). Self-adhesive resin cement can bond effectively to root dentin without additional bonding agents. However, bioactive and core build-up cements need bonding agents for optimal performance, highlighting the need to tailor bonding strategies to the specific cement used. Bonding FRPs to intra-radicular dentin was always a challenge. A strong bond to root dentin is an important factor to ensure the success and longevity of post and core restorations. This study provides great evidence for the significant influence of adhesive systems and resin cements on the bond strength of FRPs to root dentin. Using this study, clinicians will perform an informed choice of restorative materials for each clinical situation and select the best adhesive/cement combo to achieve good bond strength.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation of a Hard Photocatalytic Antibacterial TiO2 Layer on Ti Surface via Anodization in Hot Nitrate/Ethylene Glycol Electrolyte","authors":"Naofumi Ohtsu,&nbsp;Ryota Kawakami,&nbsp;Mitsuhiro Hirano","doi":"10.1002/jbm.b.35658","DOIUrl":"10.1002/jbm.b.35658","url":null,"abstract":"<div>\u0000 \u0000 <p>Preventing bacterial infections on Ti-based medical products is crucial, driving the need for durable antibacterial surfaces with enhanced mechanical strength and photocatalytic activity. This study introduces an anodization process for fabricating a hard barrier-type TiO₂ layer on a Ti substrate with visible-light-responsive photocatalytic activity. The core technology involves using an electrolyte comprising nitrate and ethylene glycol maintained at a high temperature to improve the layer hardness and photocatalytic performance. The layer characteristics, including thickness, crystallinity, and density, sensitively varied with increasing electrolyte temperature. For instance, raising the temperature to 100°C increased the layer thickness and density. By contrast, the thickness decreased beyond 100°C, leading to the deterioration of photocatalytic performance. Using ethylene glycol containing 100 mM nitrate maintained around 100°C was appropriate for maximizing layer hardness and photocatalytic performance. The resulting monolithic TiO₂ layer exhibited a hardness of ~450 HV, approximately twice that of the Ti substrate. Moreover, it effectively reduced the number of living <i>Escherichia coli</i> to ~4/100 under ultraviolet (UV) light and ~4/10 under visible light after 4 h of illumination. These results provide a guideline for obtaining a semi-permanent antibacterial medium through anodization.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Characterization of Amoxicillin-Functionalized Ag/AgCl Nanoparticles: A Promising Multifunctional Platform for Next-Generation Nanomedicine","authors":"Salah Eddine Laouini,&nbsp;Abderrhmane Bouafia,&nbsp;Manel Azzi,&nbsp;Ibtissam Laib,&nbsp;Mamoun Fellah,&nbsp;Mahmood M. S. Abdullah,&nbsp;Hamad A. Al-Lohedan,&nbsp;Johar Amin Ahmed Abdullah","doi":"10.1002/jbm.b.35661","DOIUrl":"10.1002/jbm.b.35661","url":null,"abstract":"<div>\u0000 \u0000 <p>This study synthesized and characterized amoxicillin-functionalized Ag/AgCl nanoparticles (Amoxicillin@Ag/AgCl NPs) for biomedical applications. The nanoparticles were prepared via a coprecipitation method and functionalized with amoxicillin to enhance therapeutic potential. Characterization techniques (X-ray diffraction [XRD], Fourier-transform infrared (FTIR), scanning electron microscopy [SEM], and UV–Vis) confirmed successful functionalization and improved physicochemical properties. The crystallite size increased from 17.29 ± 3.44 to 20.47 ± 4.17 nm, while the bandgap widened from 2.33 to 2.40 eV, indicating enhanced electronic interactions. Antioxidant activity was significantly improved, with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging reaching 97.87% and β-carotene bleaching inhibition at 95.47% (175 μg/mL). The antibiofilm efficacy was notable, with inhibition rates of 90.24% for <i>E. coli</i> (250 μg/mL), 81.47% for <i>S. typhimurium</i> (175 μg/mL), and 87.68% for <i>B. subtilis</i> (250 μg/mL). In enzymatic inhibition studies, Amoxicillin@Ag/AgCl NPs showed neuroprotective potential, inhibiting acetylcholinesterase (AChE) (93.74% at 160 μg/mL) and butyrylcholinesterase (BChE) (97.41% at 80 μg/mL), highlighting their potential in Alzheimer's treatment. Additionally, they exhibited anti-inflammatory effects, inhibiting lipoxygenase (LOX) by 90.47% (120 μg/mL). To the best of our knowledge, this is the first report on the synthesis of Amoxicillin@Ag/AgCl NPs that simultaneously demonstrate strong antioxidant, antibiofilm, neuroprotective, and anti-inflammatory properties, underscoring their novelty as next-generation nanomedicines.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the 3D Bioprinting Landscape in the Delivery of Active Pharmaceutical Compounds for Therapeutic and Regenerative Medicine Applications","authors":"Khonzisizwe Somandi,&nbsp;Yahya E. Choonara","doi":"10.1002/jbm.b.35654","DOIUrl":"https://doi.org/10.1002/jbm.b.35654","url":null,"abstract":"<p>Three-dimensional (3D) bioprinting is transforming the delivery of active pharmaceutical compounds and regenerative medicine by enabling patient-specific solutions that enhance treatment efficacy and safety. This review explores recent advancements in 3D bioprinting for targeted therapy, focusing on its ability to fabricate complex delivery systems of drugs, cells, and various biomolecules with controlled and sustained release profiles. By leveraging bioinks with tunable properties, 3D bioprinting allows for localized drug administration, reducing systemic side effects while improving bioavailability. Additionally, in situ 3D bioprinting facilitates the direct deposition of therapeutic agents at the site of injury or disease, enhancing precision medicine approaches and supporting tissue regeneration. The integration of biocompatible bioinks and nanomedicines minimizes toxicity, enhances drug retention, reduces adverse effects, and enables personalized treatments, significantly improving therapeutic outcomes and, in some cases, improving pharmacokinetics. Despite these advancements, challenges remain in obtaining ideal biomaterial properties, post-printing modifications, printability, and biodegradability, which are critical for clinical translation. Addressing these barriers will be key to expanding the application of 3D bioprinting in precision medicine. This review provides insights into the recent pre-clinical progress, current clinical milestones, limitations, and future directions of 3D bioprinted delivery systems of active pharmaceutical compounds, highlighting their potential to revolutionize patient-centered therapies.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35654","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}