Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Melt-Blown Polypropylene Membrane Modification for Enhanced Hydrophilicity","authors":"M. Lam,&nbsp;M. Baudoin,&nbsp;B. Mougin,&nbsp;C. Falentin-Daudré","doi":"10.1002/jbm.b.35509","DOIUrl":"10.1002/jbm.b.35509","url":null,"abstract":"<div>\u0000 \u0000 <p>Melt-blown, an environmentally friendly technique, is widely used to create high-quality non-woven fabrics by extruding molten polymer resins into interlaced fibers. In the realm of biomedical textiles, its unique microstructure makes it ideal for filtration and wound dressings. Our study focuses on modifying the surfaces of polypropylene melt-blown membranes. An effective, one-step, suitable, and reliable method to graft a bioactive polymer, sodium polystyrene sulfonate—PolyNaSS, onto the membranes has been developed. The process involves UV irradiation to initiate direct and progressive growth of NaSS over the surface through radical polymerization. To assess the efficiency of the grafting, techniques like colorimetry, water contact angle measurements, Fourier-transformed infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) were used. Outcomes related to the grafting were demonstrated by a change in wettability and quantitatively calculated sulfonate groups. Subsequently, grafted PolyNaSS promoted cell adhesion, as evidenced by improved cell morphology. On grafted membranes, fibroblasts exhibited a stretched shape, while non-grafted ones showed inactive round shapes. These findings underscore the chemical and biological reactivity of polypropylene materials, opening exciting possibilities for various applications of melt-blown materials.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc Oxide Nanoparticles Incorporated in Poly-Hydroxyethyl Methacrylate/Acrylamide Membrane Trigger the Key Events of Full-Thickness Wound Healing in a Rabbit Model","authors":"Sankhadip Saha,&nbsp;Suman Roy,&nbsp;Sonali Jana,&nbsp;Tuhin Sarkar,&nbsp;Swapno Chanda,&nbsp;Pradyot Datta,&nbsp;Bijayashree Chakraborty,&nbsp;Samiran Mondal,&nbsp;Samar Halder,&nbsp;Biswanath Kundu,&nbsp;Samit Kumar Nandi","doi":"10.1002/jbm.b.35510","DOIUrl":"10.1002/jbm.b.35510","url":null,"abstract":"<div>\u0000 \u0000 <p>Zinc oxide nanoparticles are known to possess anti-inflammatory, antibacterial, and antiseptic properties and find wide application in the preparation of topical ointments. Wound dressings in the form of hydrogels can replenish the wound microenvironment to aid the healing process in a multidimensional way. We have fabricated a composite hydrogel using 1–3 wt. % ZnO nano-particles, synthesized by chelation reaction, and poly-2-hydroxyethyl methacrylate (pHEMA)/acrylamide, synthesized, and co-polymerized in 8 kGy gamma irradiation. Developed powders and composite membranes have been thoroughly analyzed for XRD, FTIR, SEM–EDX mapping, DTA/TGA, particle size, shape, morphology, porosity, water uptake, and contact angle. Thermally stable phase-pure ZnO spherical nanoparticles with an average crystallite size of 40 ± 2 nm have been used for fabricating well-dispersed composite with contact angle varying 78^o–88^o. These membranes, when used in vivo, rendered a suitable environment conducive to tissue regeneration and ECM component deposition sequentially. Endowed with antibacterial properties, these hydrogels also demonstrated excelling swelling capacity which proved beneficial in maintaining a moist wound environment aiding in the healing process. An earlier wound closure was achieved with 2%–3% ZnO-pHEMA/acrylamide hydrogels which demonstrate the potential of ZnO nanoparticles in signaling and instructing the wound bed milieu towards the efficient repair.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Electroconductive PPy-PCL Scaffolds for Enhanced Tissue Engineering Performance","authors":"Ana M. Muñoz-González,&nbsp;Dianney Clavijo-Grimaldo,&nbsp;Sara Leal-Marin,&nbsp;Birgit Glasmacher","doi":"10.1002/jbm.b.35511","DOIUrl":"10.1002/jbm.b.35511","url":null,"abstract":"<div>\u0000 \u0000 <p>The integration of electrically conductive materials is a promising approach in tissue regeneration research. The study presented focuses on the creation of electroconductive scaffolds made from polypyrrole-polycaprolactone (PPy-PCL) using optimal processing parameters. Utilizing Box–Behnken response surface methodology for in situ chemical polymerization of PPy, the scaffolds exhibited a maximum conductivity of 2.542 mS/cm. Morphological examination via scanning electron microscopy (SEM) indicated uniform dispersion of PPy particles within PCL fibers. Fourier transform infrared spectroscopy (FTIR) and energy dispersive x-ray (EDX) analysis validated the composition of the scaffolds, while mechanical testing revealed that the optimized scaffolds exhibit superior tensile strength and Young's modulus compared to scaffolds comprised only of PCL. The hydrophilicity of the scaffolds was improved considerably, transitioning from initially hydrophobic to fully hydrophilic for the optimum scaffold, making it suitable for tissue engineering applications. Cell viability assays, including MTT with L929 fibroblasts and Alamar Blue with bone marrow mesenchymal stem cells (bmMSCs), reflected no cytotoxicity. They showed an increase in metabolic activity, suggesting the capability of the scaffolds to support cellular functions. In conclusion, the in situ synthesis of PPy in the PCL matrix by optimizing the fabrication parameters resulted in conductive scaffolds with promising structural and functional properties for tissue engineering.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative Anticoagulant Strategy to Improve the Test Sensitivity of ASTM F2888-19 Standard for Platelet and Leukocyte Count Assay","authors":"Mehulkumar Patel,&nbsp;Carlos Serna III,&nbsp;Anna Parrish,&nbsp;Arjun Gupta,&nbsp;Megan Jamiolkowski,&nbsp;Qijin Lu","doi":"10.1002/jbm.b.35514","DOIUrl":"10.1002/jbm.b.35514","url":null,"abstract":"<div>\u0000 \u0000 <p>The ASTM F2888-19 standard for platelet and leukocyte count assay is the only standardized test method for assessing platelet and leukocyte interactions with blood-contacting device materials. This study aimed to address two limitations of the ASTM F2888-19 standard: low test sensitivity for leukocyte count and high test sample surface area to blood ratio (12 cm²/mL). Human blood from healthy adult donors was drawn into polypropylene tubes with either 3.2% sodium (Na) citrate or anticoagulant citrate dextrose solution A (ACDA). Immediately before starting the test, the blood was recalcified and heparinized to a concentration of 1, 1.5, or 2 U/mL and incubated with the test materials of varying thrombogenic potential at an exposure ratio of 6 or 12 cm²/mL for 1 h at 37°C ± 2°C in a shaking water bath. Complete blood count was measured using a hematology analyzer. The results show that both, Na-citrated blood (6 or 12 cm²/mL exposure ratio) and ACDA blood (6 cm²/mL ratio), were able to differentiate thrombogenic materials from commonly used biomaterials based on platelet count changes. The magnitudes of difference between the thrombogenic materials and biomaterials depends on heparin concentration. The test sensitivity was highest when ACDA blood, heparinized to 1 U/mL heparin, was used. Moreover, the use of ACDA blood, unlike Na-citrated blood, also allowed the assay to distinguish between the thrombogenic materials from commonly used biomaterials based on leukocyte count changes. In conclusion, the use of ACDA blood significantly increased test sensitivity of the ASTM F2888-19 test method in differentiating materials with varying thrombogenicity based on both platelet and leukocyte counts, while reducing blood exposure ratio to 6 cm²/mL. These findings will be used to revise the ASTM F2888 standard in the future.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atomic Interaction S. aureus/Machined and Additive Manufacturing Ti-6Al-4V and Ti-35Nb-7Zr-5Ta Disks for Dental Implants","authors":"Juliana Dias Corpa Tardelli,&nbsp;Lucas Barcelos Otani,&nbsp;Rodolfo Lisboa Batalha,&nbsp;Fernanda Alves,&nbsp;Marcelo A. Pereira-da-Siva,&nbsp;Vanderlei Salvador Bagnato,&nbsp;Piter Gargarella,&nbsp;Claudemiro Bolfarini,&nbsp;Andréa Cândido dos Reis","doi":"10.1002/jbm.b.35508","DOIUrl":"10.1002/jbm.b.35508","url":null,"abstract":"<div>\u0000 \u0000 <p>The adhesion strength of a bacterial strain on a substrate influences colonization and biofilm development, so the biomolecular analysis of this interaction is a step that allows insights into the development of antifouling surfaces. As peri-implantitis is the main cause of failure of implant-supported oral rehabilitations and the dental literature presents gaps in the atomic bacteria/surface interaction, this study aimed to correlate the qualitative variation of roughness, wettability, chemical composition, and electrical potential of Ti-6Al-4V and Ti-35Nb-7Zr-5Ta (TNZT) disks obtained by machining (M) and additive manufacturing (AM) on the colonization and adhesion strength of <i>S. aureus</i> quantified by atomic force microscopy (AFM). The samples were evaluated for roughness, electrical potential, and <i>S. aureus</i> colonization and adhesion strength by specific methods in the AFM with subsequent analysis in the NanoScope software analysis, wettability by sessile drop method, and chemical composition by energy dispersive x-ray spectroscopy (EDX). Qualitative data were correlated with bacterial adhesion strength. The greater adhesion strength of <i>S. aureus</i> was observed in descending order for TNZT AM, TNZT M, Ti-6Al-4V AM, and Ti-6Al-4V M. This experimental in vitro study allowed us to conclude that for the evaluated groups, the strength adhesion of <i>S. aureus</i> showed a linear relationship with roughness, and nonlinear for wettability, electrical potential, and <i>S. aureus</i> colonization on the surfaces evaluated. As for the two variation factors, type of alloy and manufacturing method, those that promoted the lowest bacterial adhesion strength were Ti-6Al-4V and M, possibly attributed to the synergistic modification of the evaluated surface properties. Thus, this study suggests <i>S. aureus</i> preferences for rough, hydrophilic surfaces with a greater electrical potential difference.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tibial Baseplate Microstructure Governs High Cycle Fatigue Fracture In Vivo","authors":"Michael A. Kurtz,&nbsp;Jeremy L. Gilbert,&nbsp;Hannah Spece,&nbsp;Gregg R. Klein,&nbsp;Harold E. Cates,&nbsp;Steven M. Kurtz","doi":"10.1002/jbm.b.35507","DOIUrl":"10.1002/jbm.b.35507","url":null,"abstract":"<p>Previous studies report rare occurrences of tibial baseplate fractures following primary total knee arthroplasty (TKA). However, at a microstructural scale, it remains unclear how fatigue models developed in vitro apply to fractures in vivo. In this study, we asked: (1) do any clinical factors differentiate fracture patients from a broader revision sample; and (2) in vivo, how does microstructure influence fatigue crack propagation? We identified three fractured tibial baseplates from an institutional review board exempt implant retrieval program. Then, for comparison, we collated clinical data from the same database for <i>n</i> = 2120 revision TKA patients with tibial trays. To identify mechanisms, we characterized fracture features using scanning electron and digital optical microscopy. Additionally, we performed cross sectional analysis using focused ion beam milling. The fracture cohort consisted of moderately to very active patients with increased implantation time (15.6 years) compared to the larger revision patient sample (5.1 years, <i>p</i> = 0.009). We did not find a significant difference in patient weight between the two groups (<i>p</i> = 0.98). Macroscopic fracture features aligned well with both previous retrieval analysis and in vitro baseplate fatigue tests. On a micron scale, we identified striations on each baseplate, demonstrating fatigue as the fracture mechanism. In vivo fatigue fracture processes depended on both the alloy (Ti-6Al-4V vs. CoCrMo) and the microstructure of the alloy formed during manufacturing. For Ti-6Al-4V, the presence of equiaxed or acicular microstructure influenced the fatigue crack propagation, the latter arising from large prior β grains and a Widmanstatten microstructure, degrading fatigue strength. CoCrMo alloy fatigue cracks propagated linearly, crystallographically influenced by planar slip. However, we did not document any features of overload or fast fracture, suggesting a high cycle, low stress fatigue regime. Ultimately, the crack profiles we present here may provide insight into fatigue fractures of modern tibial baseplates.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wear, Material Transfer, and Electrocautery Damage Are Ubiquitous on CoCrMo Femoral Knee Retrievals","authors":"Peter W. Kurtz,&nbsp;Michael A. Kurtz,&nbsp;Shabnam Aslani,&nbsp;Lilliana M. Taylor,&nbsp;Charley M. Goodwin,&nbsp;Daniel W. MacDonald,&nbsp;Nicolas S. Piuzzi,&nbsp;William M. Mihalko,&nbsp;Steven M. Kurtz,&nbsp;Jeremy L. Gilbert","doi":"10.1002/jbm.b.35504","DOIUrl":"https://doi.org/10.1002/jbm.b.35504","url":null,"abstract":"<p>Despite high total knee arthroplasty (TKA) survivorship after 10 years (92%–99%), a gap persists where patient satisfaction lags clinical success. Additionally, while cobalt chrome molybdenum (CoCrMo) use decreases in primary total hip arthroplasty, the alloy continues to be widely used in TKA femoral components. In vivo, CoCrMo degradation may be associated with adverse local tissue reactions (ALTR) and compared with the hip, the damage mechanisms that may release metal in the knee and the potential biological effects remain poorly understood. In this study, we characterized the damage on 50 retrieved CoCrMo femoral knee implants paired with 19 titanium alloy and 31 CoCrMo tibial baseplates. We asked (1) what damage modes can release CoCrMo debris in vivo from femoral components and (2) how frequently does the damage occur? First, we developed a semiquantitative scoring system for abrasive wear. Then, we characterized damage modes on CoCrMo femoral implants using digital optical microscopy (DOM), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). We found that wear, electrocautery damage, and Ti-6Al-4V material transfer were ubiquitous. Of the 50 CoCrMo femoral implants we investigated, we documented wear on 100% (<i>n</i> = 50/50), electrocautery damage on 98% (<i>n</i> = 49/50), and Ti-6Al-4V material transfer to the posterior condyles on 95% (<i>n</i> = 18/19). Our results suggest that these damage modes may be more prevalent than previously thought and may act as metal release mechanisms in vivo.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35504","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to Physically-Cross-Linked Poly(Vinyl Alcohol) Cell Culture Plate Coatings Facilitate Preservation of Cell–Cell Interactions, Spheroid Formation, and Stemness","authors":"","doi":"10.1002/jbm.b.35506","DOIUrl":"https://doi.org/10.1002/jbm.b.35506","url":null,"abstract":"<p>K. Molyneaux, M. D. Wnek, S. E. L. Craig, et al., “Physically-Cross-Linked Poly(Vinyl Alcohol) Cell Culture Plate Coatings Facilitate Preservation of Cell–Cell Interactions, Spheroid Formation, and Stemness,” Journal of Biomedical Materials Research. Part B, Applied Biomaterials 109, no. 11 (2021):1744–1753.</p><p>FIGURE 4 Immunostaining of spheroids from long-term cultures indicate presence of various cell lineages and tumor biomarkers. Day 18 LN229 spheres derived from culture in 60 mm dishes were sectioned and processed for immunohistochemistry for neuronal, stem cell, glial, vascular, mesenchymal, and tumor biomarkers. LN229 spheres express markers of neuronal stem cells (FoxG1 and Zeb 1) and vascular (CD31) and mesenchymal (vimentin) populations but not glial cells (note absence of GFAP staining). There is also some expression of a neural marker (βIII tubulin). In addition, staining with a tumor biomarker to PTPμ, SBK4, was also present. A phase contrast image of an 18-day aggregate is also shown.</p><p>We apologize for this error.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35506","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the Construction and Application of Bone-on-a-Chip Based on Microfluidic Technologies","authors":"Chang Zhong,&nbsp;Zihui Tang,&nbsp;Xin Yu,&nbsp;Lu Wang,&nbsp;Chenyuan Ren,&nbsp;Liying Qin,&nbsp;Ping Zhou","doi":"10.1002/jbm.b.35502","DOIUrl":"10.1002/jbm.b.35502","url":null,"abstract":"<div>\u0000 \u0000 <p>Bone-on-a-chip (BOC) models that based on microfluidic technology have widely applied to understand bone physiology and the pathogenesis of related diseases. In this review, we provide an overview of bone biology and related diseases, explain the advantages and applications of microfluidic technology in the construction of BOC models, and summarize their progress in physiology, pathology, and drug development. Finally, we discussed the problems to be solved and the future directions of microfluidic technology and BOC platforms, so as to provide a reference for researchers to design better BOC models.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additively Manufactured 3D Clamp-Culture System for the Investigation of Material-Cell Interactions in Multi-Material Hybrid Scaffolds for Musculoskeletal Tissue Defects","authors":"Simon Enbergs,&nbsp;Lennard K. Shopperly,&nbsp;Andreas Engels,&nbsp;Dominik Laue,&nbsp;Wolfgang Ertel,&nbsp;Michael Sittinger,&nbsp;Carsten Rendenbach,&nbsp;Tilo Dehne,&nbsp;Michal Jagielski,&nbsp;Jacob Spinnen","doi":"10.1002/jbm.b.35494","DOIUrl":"10.1002/jbm.b.35494","url":null,"abstract":"<p>The emergence of hybrid scaffolds, blending biomaterials with diverse properties, offers promise in musculoskeletal tissue engineering. However, a need for in vitro platforms investigating biological behavior and the interplay of different load-bearing and colonizable synthetic bone substitute materials remains. Herein, we present a novel, in-house producible, and scalable clamp culture system designed for facile in vitro analysis of interactions between biomaterials, hydrogels, and cells. The system, constructed here from an exemplary 3D-printable polymer and photopolymerizable hydrogel using a widely available benchtop 3D printer, ensures mechanical stability and protection for the embedded hydrogel via its double-clamp structure, facilitating various analytical methods while preserving culture integrity. Hybrid clamp cultures were additively manufactured from polylactic acid, filled with a bone precursor cell-laden methacrylate gelatin hydrogel, cultured for 14 days, and analyzed for cell viability, mineralization, and osseous differentiation. Results indicate no adverse effects on osteogenic differentiation or mineralization compared to conventional droplet cultures, with enhanced cell viability and simplified handling and downstream analysis. This system demonstrates the potential for robust experimentation in tissue engineering and is adaptable to various plate formats, and thus highly suitable for the investigation of biomaterial-cell interactions and the development of implants for musculoskeletal tissue defects.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}