Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Correction to: Systematic Enhancement of Microbial Decontamination Efficiency in Bone Graft Processing by Means of High Hydrostatic Pressure Using Escherichia coli as a Model Organism","authors":"","doi":"10.1002/jbm.b.35505","DOIUrl":"10.1002/jbm.b.35505","url":null,"abstract":"<p>H. Loeffler, J. Waletzko-Hellwig, R. Fischer, et al., “Systematic Enhancement of Microbial Decontamination Efficiency in Bone Graft Processing by Means of High Hydrostatic Pressure Using Escherichia coli as a Model Organism,” Journal of Biomedical Materials Research Part B 112 (2024): e35383. https://doi.org/10.1002/jbm.b.35383.</p><p>A recalibration of the high hydrostatic pressure device after the publication of this paper revealed incorrect pressure levels stated in this article. As this is a systematic error, it is necessary to correct the pressure levels by the factor of 0.7. Accordingly, 250 MPa now corresponds to 175 MPa, 350 MPa was corrected to 245 MPa and 600 MPa is now 420 MPa, suggesting that the efficiency of the treatment was higher than initially assumed.</p><p>A detailed listing of the affected text passages can be found below. Figures as well as Figure captions were corrected and attached, accordingly.</p><p>We apologize for this error.\u0000 </p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of Empty Lacunae in Tissue Sections of Osteonecrosis of the Femoral Head Using YOLOv8 Artificial Intelligence Model","authors":"Issei Shinohara,&nbsp;Atsuyuki Inui,&nbsp;Masatoshi Murayama,&nbsp;Yosuke Susuki,&nbsp;Qi Gao,&nbsp;Simon Kwoon-Ho Chow,&nbsp;Yutaka Mifune,&nbsp;Tomoyuki Matsumoto,&nbsp;Ryosuke Kuroda,&nbsp;Stuart B. Goodman","doi":"10.1002/jbm.b.35512","DOIUrl":"https://doi.org/10.1002/jbm.b.35512","url":null,"abstract":"<div>\u0000 \u0000 <p>Histomorphometry is an important technique in the evaluation of non-traumatic osteonecrosis of the femoral head (ONFH). Quantification of empty lacunae and pyknotic cells on histological images is the most reliable measure of ONFH pathology, yet it is time and manpower consuming. This study focused on the application of artificial intelligence (AI) technology to tissue image evaluation. The aim of this study is to establish an automated cell counting platform using YOLOv8 as an object detection model on ONFH tissue images and to evaluate and validate its accuracy. From 30 ONFH model rabbits, 270 tissue images were prepared; based on evaluations by three researchers, ground truth labels were created to classify each cell in the image into two classes (osteocytes and empty lacunae) or three classes (osteocytes, pyknotic cells, and empty lacunae). Two and three classes were then annotated on each image. Transfer learning based on annotated data (80% for training and 20% for validation) was performed using YOLOv8n and YOLOv8x with different parameters. To evaluate the detection accuracy of the training model, the mean average precision (mAP (50)) and precision-recall curve were identified. In addition, the reliability of cell counting by YOLOv8 relative to manual cell counting was evaluated by linear regression analysis using five histological images unused in previous experiments. The mAP (50) for the detection of empty lacunae was 0.868 for the YOLOv8n and 0.883 for the YOLOv8x. The mAP (50) for the three classes was 0.735 for the YOLOv8n model and 0.750 for the YOLOv8x model. The quantification of empty lacunae by automated cell counting obtained in the learning was highly correlated with the manual counting data. The development of an AI-applied automated cell counting platform will significantly reduce the time and effort of manual cell counting in histological analysis.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Titanium Implant Modified With Zinc-Doped Carbon Dot Layer as an Innovative Coating for the Development of Local Drug Delivery System for Ciprofloxacin","authors":"Jakub Reczkowski,&nbsp;Łukasz Ławniczak,&nbsp;Maria Ratajczak,&nbsp;Adam Voelkel,&nbsp;Mariusz Sandomierski","doi":"10.1002/jbm.b.35517","DOIUrl":"10.1002/jbm.b.35517","url":null,"abstract":"<div>\u0000 \u0000 <p>This study presents a new innovative drug delivery system for ciprofloxacin, which is based on the formation of a zinc-doped carbon dots layer on the surface of a titanium alloy (TiAl4V6). In the study, the effectiveness of the synthesis method of a zinc-doped carbon dots layer was determined. The distribution of the layer of carbon dots on the surface of the titanium alloy was investigated using the FT-IR mapping technique, which confirmed the efficiency of the synthesis. The effective synthesis of carbon dots and the coordination of zinc ions on their surface opens the possibility of sorption of ciprofloxacin, which results in a high application potential of the obtained biomaterial. The introduction of zinc cations on the surface of the carbon dots layer resulted in high sorption results of the active substance (40 μg of drug per 1 cm² of implant). The release profile of ciprofloxacin from the modified surface of the titanium alloy indicates that this active substance can be released for up to 4 h. The biomaterial obtained in this work is also hydrophilic (about 40°), which was shown by the contact angle tests. This is an important feature and indicates a high application potential of the performed modification. The resulting layer has antibacterial properties. Growth inhibition for microorganisms such as <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Bacillus cereus</i>, and <i>Candida albicans</i> ranged from 74% to 96%. The creation of such a layer on the titanium alloy may reduce the risk of infection during the implantation procedure.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation and Fatigue Behavior of 3D-Printed Bioresorbable Tracheal Splints","authors":"Jenna M. Wahbeh,&nbsp;John Lama,&nbsp;Sang-Hyun Park,&nbsp;Edward Ebramzadeh,&nbsp;Scott J. Hollister,&nbsp;Sophia N. Sangiorgio","doi":"10.1002/jbm.b.35501","DOIUrl":"10.1002/jbm.b.35501","url":null,"abstract":"<p>Severe infantile tracheobronchomalacia (TBM) is often treated with invasive surgery and fixed-size implants to support the trachea during respiration. A novel 3D-printed extra-luminal splint has been developed as a flexible and bioresorbable alternative. Therefore, the goal of the present study was to use an in vitro breathing simulator model to comprehensively evaluate the structural stiffness and failure modes of two sizes of a novel bioresorbable 3D-printed splint design under a range of physiological degradation conditions. Two thicknesses, 2 mm and 3 mm, of a novel 3D-printed bioresorbable splint were evaluated under two different degradation conditions, phosphate-buffered saline (PBS) and sodium hydroxide (NaOH). The splints were subjected to simulated breathing loading, involving a cyclic opening and closing of the splint by 2 mm, for a targeted duration of 7.5 to 30 million cycles. A separate new set of splints were statically soaked in their respective degradation condition for a comparative analysis of the effects of cyclic loading by the degradation medium. After successfully simulated breathing or static soaking, non-destructive tensile and compressive strengths were evaluated, and overall stiffness was calculated from destructive tensile testing. The present study indicates that the splints were more significantly degraded under simulated breathing conditions than under soaking. Cyclic simulated breathing specimens failed far earlier than the intended duration of loading. Over time, both 2 mm and 3 mm splints became increasingly more flexible when subjected to the static degradation conditions. Interestingly, there was little difference in the compressive and tensile strengths of the 2 mm and 3 mm thickness splints. The bioresorbable nature of PCL offers a valuable advantage as it eliminates the need for splint removal surgery and increases device flexibility over time with degradation. This increased flexibility is crucial because it allows for uninhibited growth and development of the infant's trachea over the intended use period of 2 years. The results of this study confirm that the splints were able to withstand tensile forces to prevent tracheal collapse. This study further supports the successful use of 3D-printed splints in the treatment of infantile TBM.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35501","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An In Vivo Assessment of Different Mesenchymal Stromal Cell Tissue Types and Their Differentiation State on a Shape Memory Polymer Scaffold for Bone Regeneration","authors":"Teja Guda,&nbsp;Jessica M. Stukel Shah,&nbsp;Bridney D. Lundquist,&nbsp;Joseph M. Macaitis,&nbsp;Mística Lozano Pérez,&nbsp;Michaela R. Pfau-Cloud,&nbsp;Felipe O. Beltran,&nbsp;Connie W. Schmitt,&nbsp;Emily M. Corbin,&nbsp;Melissa A. Grunlan,&nbsp;Wen Lien,&nbsp;Heuy-Ching Wang,&nbsp;Alexander J. Burdette","doi":"10.1002/jbm.b.35516","DOIUrl":"10.1002/jbm.b.35516","url":null,"abstract":"<div>\u0000 \u0000 <p>A combined biomaterial and cell-based solution to heal critical size bone defects in the craniomaxillofacial area is a promising alternative therapeutic option to improve upon autografting, the current gold standard. A shape memory polymer (SMP) scaffold, composed of biodegradable poly(ε-caprolactone) and coated with bioactive polydopamine, was evaluated with mesenchymal stromal cells (MSCs) derived from adipose (ADSC), bone marrow (BMSC), or umbilical cord (UCSC) tissue in their undifferentiated state or pre-differentiated toward osteoblasts for bone healing in a rat calvarial defect model. Pre-differentiating ADSCs and UCSCs resulted in higher new bone volume fraction (15.69% ± 1.64%) compared to empty (i.e., untreated) defects and scaffold-only (i.e., unseeded) groups (4.41% ± 1.11%). Notably, only differentiated UCSCs exhibited a significant increase in new bone volume, surpassing both undifferentiated UCSCs and unseeded scaffolds. Further, differentiated ADSCs and UCSCs had significantly higher trabecular numbers than their undifferentiated counterparts, unseeded scaffolds, and untreated defects. Although the mineral density regenerated within the unseeded scaffold surpassed that achieved with cell seeding, the connectivity of this bone was diminished, as the regenerated tissue confined itself to the spherical morphology of the scaffold pores. The SMP scaffold alone, with undifferentiated BMSCs, with undifferentiated and differentiated ADSCs, and differentiated UCSCs (29.72 ± 1.49 N) demonstrated significant osseointegration compared to empty defects (14.34 ± 2.21 N) after 12 weeks of healing when assessed by mechanical push-out testing. Based on these results and tissue availability to obtain the cells, pre-differentiated ADSCs and UCSCs emerge as particularly promising candidates when paired with the SMP scaffold for repairing critical size bone defects in the craniofacial skeleton.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shape Memory Polymer Scaffolds—Utility for In Vitro Osteogenesis of Canine Multipotent Stromal Cells","authors":"Shelby B. Gasson,&nbsp;Lauren K. Dobson,&nbsp;Michaela R. Pfau-Cloud,&nbsp;Felipe O. Beltran,&nbsp;Carl A. Gregory,&nbsp;Melissa A. Grunlan,&nbsp;W. Brian Saunders","doi":"10.1002/jbm.b.35503","DOIUrl":"10.1002/jbm.b.35503","url":null,"abstract":"<div>\u0000 \u0000 <p>A biodegradable, shape memory polymer (SMP) scaffold based on poly(ε-caprolactone) (PCL) represents an attractive alternative therapy for the repair of critically sized bone defects given its ability to press-fit within irregular defects. Clinical translation of SMP scaffolds requires successful movement beyond proof-of-concept rodent studies through a relevant large-animal model and into the clinical setting. In addition to representing a clinical veterinary population, the canine species is a strong translational model for humans due to similarities in orthopedic disorders, biomechanics, and bone healing. The present study was performed to assess in vitro cytocompatibility and osteogenic differentiation of canine multipotent stromal cells (cMSCs) cultured on SMP scaffolds in preparation for future canine in vivo studies. Two different SMP scaffold compositions were utilized: a “PCL-only” scaffold prepared from PCL-diacrylate (PCL-DA) and a semi-interpenetrating network (semi-IPN) formed from PCL-DA and poly(L-lactic acid) (PCL:PLLA). The PCL:PLLA scaffolds degrade faster and are more mechanically rigid versus the PCL scaffolds. Canine bone marrow–derived MSCs (cMSCs) were evaluated in terms of attachment, proliferation, and osteogenic differentiation. cMSCs exhibited excellent cytocompatibility, attachment, and proliferation on both SMP scaffold compositions. PCL scaffolds were more conducive to both early- and late-stage in vitro osteogenesis of cMSCs versus PCL:PLLA scaffolds. However, cMSCs deposited mineralized extracellular matrix over 21 days when cultured on both SMP scaffold compositions. These results demonstrate that the SMP scaffolds are suitable for in vitro cMSC attachment, proliferation, and osteogenic differentiation, representing a significant step toward canine in vivo studies and potential translation to human patients.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melt-Blown Polypropylene Membrane Modification for Enhanced Hydrophilicity","authors":"M. Lam,&nbsp;M. Baudoin,&nbsp;B. Mougin,&nbsp;C. Falentin-Daudré","doi":"10.1002/jbm.b.35509","DOIUrl":"10.1002/jbm.b.35509","url":null,"abstract":"<div>\u0000 \u0000 <p>Melt-blown, an environmentally friendly technique, is widely used to create high-quality non-woven fabrics by extruding molten polymer resins into interlaced fibers. In the realm of biomedical textiles, its unique microstructure makes it ideal for filtration and wound dressings. Our study focuses on modifying the surfaces of polypropylene melt-blown membranes. An effective, one-step, suitable, and reliable method to graft a bioactive polymer, sodium polystyrene sulfonate—PolyNaSS, onto the membranes has been developed. The process involves UV irradiation to initiate direct and progressive growth of NaSS over the surface through radical polymerization. To assess the efficiency of the grafting, techniques like colorimetry, water contact angle measurements, Fourier-transformed infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) were used. Outcomes related to the grafting were demonstrated by a change in wettability and quantitatively calculated sulfonate groups. Subsequently, grafted PolyNaSS promoted cell adhesion, as evidenced by improved cell morphology. On grafted membranes, fibroblasts exhibited a stretched shape, while non-grafted ones showed inactive round shapes. These findings underscore the chemical and biological reactivity of polypropylene materials, opening exciting possibilities for various applications of melt-blown materials.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc Oxide Nanoparticles Incorporated in Poly-Hydroxyethyl Methacrylate/Acrylamide Membrane Trigger the Key Events of Full-Thickness Wound Healing in a Rabbit Model","authors":"Sankhadip Saha,&nbsp;Suman Roy,&nbsp;Sonali Jana,&nbsp;Tuhin Sarkar,&nbsp;Swapno Chanda,&nbsp;Pradyot Datta,&nbsp;Bijayashree Chakraborty,&nbsp;Samiran Mondal,&nbsp;Samar Halder,&nbsp;Biswanath Kundu,&nbsp;Samit Kumar Nandi","doi":"10.1002/jbm.b.35510","DOIUrl":"10.1002/jbm.b.35510","url":null,"abstract":"<div>\u0000 \u0000 <p>Zinc oxide nanoparticles are known to possess anti-inflammatory, antibacterial, and antiseptic properties and find wide application in the preparation of topical ointments. Wound dressings in the form of hydrogels can replenish the wound microenvironment to aid the healing process in a multidimensional way. We have fabricated a composite hydrogel using 1–3 wt. % ZnO nano-particles, synthesized by chelation reaction, and poly-2-hydroxyethyl methacrylate (pHEMA)/acrylamide, synthesized, and co-polymerized in 8 kGy gamma irradiation. Developed powders and composite membranes have been thoroughly analyzed for XRD, FTIR, SEM–EDX mapping, DTA/TGA, particle size, shape, morphology, porosity, water uptake, and contact angle. Thermally stable phase-pure ZnO spherical nanoparticles with an average crystallite size of 40 ± 2 nm have been used for fabricating well-dispersed composite with contact angle varying 78^o–88^o. These membranes, when used in vivo, rendered a suitable environment conducive to tissue regeneration and ECM component deposition sequentially. Endowed with antibacterial properties, these hydrogels also demonstrated excelling swelling capacity which proved beneficial in maintaining a moist wound environment aiding in the healing process. An earlier wound closure was achieved with 2%–3% ZnO-pHEMA/acrylamide hydrogels which demonstrate the potential of ZnO nanoparticles in signaling and instructing the wound bed milieu towards the efficient repair.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Electroconductive PPy-PCL Scaffolds for Enhanced Tissue Engineering Performance","authors":"Ana M. Muñoz-González,&nbsp;Dianney Clavijo-Grimaldo,&nbsp;Sara Leal-Marin,&nbsp;Birgit Glasmacher","doi":"10.1002/jbm.b.35511","DOIUrl":"10.1002/jbm.b.35511","url":null,"abstract":"<div>\u0000 \u0000 <p>The integration of electrically conductive materials is a promising approach in tissue regeneration research. The study presented focuses on the creation of electroconductive scaffolds made from polypyrrole-polycaprolactone (PPy-PCL) using optimal processing parameters. Utilizing Box–Behnken response surface methodology for in situ chemical polymerization of PPy, the scaffolds exhibited a maximum conductivity of 2.542 mS/cm. Morphological examination via scanning electron microscopy (SEM) indicated uniform dispersion of PPy particles within PCL fibers. Fourier transform infrared spectroscopy (FTIR) and energy dispersive x-ray (EDX) analysis validated the composition of the scaffolds, while mechanical testing revealed that the optimized scaffolds exhibit superior tensile strength and Young's modulus compared to scaffolds comprised only of PCL. The hydrophilicity of the scaffolds was improved considerably, transitioning from initially hydrophobic to fully hydrophilic for the optimum scaffold, making it suitable for tissue engineering applications. Cell viability assays, including MTT with L929 fibroblasts and Alamar Blue with bone marrow mesenchymal stem cells (bmMSCs), reflected no cytotoxicity. They showed an increase in metabolic activity, suggesting the capability of the scaffolds to support cellular functions. In conclusion, the in situ synthesis of PPy in the PCL matrix by optimizing the fabrication parameters resulted in conductive scaffolds with promising structural and functional properties for tissue engineering.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative Anticoagulant Strategy to Improve the Test Sensitivity of ASTM F2888-19 Standard for Platelet and Leukocyte Count Assay","authors":"Mehulkumar Patel,&nbsp;Carlos Serna III,&nbsp;Anna Parrish,&nbsp;Arjun Gupta,&nbsp;Megan Jamiolkowski,&nbsp;Qijin Lu","doi":"10.1002/jbm.b.35514","DOIUrl":"10.1002/jbm.b.35514","url":null,"abstract":"<div>\u0000 \u0000 <p>The ASTM F2888-19 standard for platelet and leukocyte count assay is the only standardized test method for assessing platelet and leukocyte interactions with blood-contacting device materials. This study aimed to address two limitations of the ASTM F2888-19 standard: low test sensitivity for leukocyte count and high test sample surface area to blood ratio (12 cm²/mL). Human blood from healthy adult donors was drawn into polypropylene tubes with either 3.2% sodium (Na) citrate or anticoagulant citrate dextrose solution A (ACDA). Immediately before starting the test, the blood was recalcified and heparinized to a concentration of 1, 1.5, or 2 U/mL and incubated with the test materials of varying thrombogenic potential at an exposure ratio of 6 or 12 cm²/mL for 1 h at 37°C ± 2°C in a shaking water bath. Complete blood count was measured using a hematology analyzer. The results show that both, Na-citrated blood (6 or 12 cm²/mL exposure ratio) and ACDA blood (6 cm²/mL ratio), were able to differentiate thrombogenic materials from commonly used biomaterials based on platelet count changes. The magnitudes of difference between the thrombogenic materials and biomaterials depends on heparin concentration. The test sensitivity was highest when ACDA blood, heparinized to 1 U/mL heparin, was used. Moreover, the use of ACDA blood, unlike Na-citrated blood, also allowed the assay to distinguish between the thrombogenic materials from commonly used biomaterials based on leukocyte count changes. In conclusion, the use of ACDA blood significantly increased test sensitivity of the ASTM F2888-19 test method in differentiating materials with varying thrombogenicity based on both platelet and leukocyte counts, while reducing blood exposure ratio to 6 cm²/mL. These findings will be used to revise the ASTM F2888 standard in the future.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}