Allisson Benatti Justino, Serena Mares Malta, Vinicius Prado Bittar, Maria Sol Peña Carrillo, Fernanda Naves Araújo do Prado Mascarenhas, Rafael Bernardes Ferreira, Alexandre Souza Marquez, Renata Graciele Zanon, Carlos Ueira-Vieira, Foued Salmen Espindola
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引用次数: 0
Abstract
Embryonic development is highly sensitive to oxidative stress, which can disrupt homeostasis. A strategy for mitigating oxidative stress induced by gold nanoparticles (AuNPs) involves the development of nanoparticles functionalized with phytoantioxidants through green chemistry methods, which also enhances the bioavailability of these antioxidants. In this study, environmentally friendly AuNPs were synthesized using curcumin (AuNPs-C), characterized by a spherical shape, uniform size, and a diameter of 7.2 ± 1.2 nm. The effects of AuNPs-C on oxidative stress in Drosophila melanogaster (Canton-S strain) during embryonic development were investigated, focusing on antioxidant defenses, oxidative damage, and morphological changes in the gastrointestinal tract. Exposure of Drosophila eggs to 50–200 μg/mL of AuNPs-C had no effect on hatching rates or pupal/adult development, with eclosion rates remaining above 50%. AuNPs-C did not elevate reactive oxygen species levels or induce lipid and protein oxidation in larvae exposed to 200 μg/mL. Oxidized protein products and malondialdehyde (MDA) levels remained comparable to those of the control group (70 ± 3 μM chloramine-T eq and 0.8 ± 0.1 nM MDA eq, respectively). Although AuNPs-C did not affect catalase activity or glutathione content, it reduced superoxide dismutase activity by 67% ± 6%. Additionally, AuNPs-C did not cause any damage to the gastrointestinal tract or alter the gut permeability of third-instar larvae. This study offers a deeper understanding of how AuNPs-C influence oxidative stress and antioxidant defense mechanisms in animal development and provides a basis for assessing the safety of phytoantioxidant-functionalized nanoparticles in vivo.
期刊介绍:
Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats:
• original research reports
• short research and development reports
• scientific reviews
• current concepts articles
• special reports
• editorials
Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.